*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to
www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specified code number.
USE OF THE OCTET COM PARA TOR
The Octet Comparator contains eight permanent
color standards. A test sample is inserted into the
openings in the top of the comparator. The sample
can then be compared to four color standards at
once, and the value read off the comparator. For
optimum color comparison, the comparator should
be positioned between the operator and a light
source, so that the light enters through the special
light-diffusing screen in the back of the comparator.
Avoid viewing the comparator against direct
sunlight or an irregularly lighted background.
PRO CE DURE 1:
FREE AVAIL ABLE CHLO RINE, MONOCHLORAMINE,
DICHLORAMINE & TO TAL RE SID UAL CHLO RINE
FREE AVAILABLE CHLORINE
1. Rinse the test tube (0898) with sample water. Fill to the bottom line
with sample water.
2. Add one *Chlorine DPD #1R Tablet (6999A). Crush tablet with
tablet crusher (0175). Cap the test tube and mix until tablet
disintegrates.
3. Immediately insert the test tube into the Chlorine DPD Comparator,
0.1-1.0 ppm (6978) or the Chlorine DPD Comparator, 1.0-6.0 ppm
(6979). Match sample color to a color standard. Color matching
should be completed within one minute from the addition of the
*Chlorine DPD #1R Tablet. This is the Free Available Chlorine
concentration of the test sample. Record as Reading A.
4. Retain this test sample for the Monochloramine Determination.
MONOCHLORAMINE
5. To the test sample from Step 4 above, add one Chlorine DPD #2R
Tablet (6904A). Crush tablet with tablet crusher (0175). Cap the test
tube and mix until tablet disintegrates.
6. Insert the test tube into the Chlorine DPD Comparator, 0.1-1.0 ppm
(6978) or the Chlorine DPD Comparator, 1.0-6.0 ppm (6979).
Match sample color to a color standard. Record as Reading B. Any
increase in color over Reading A is due to Monochloramine.
Reading B - Reading A = Monochloramine (ppm)
7. Retain this test sample for the Dichloramine determination.
DICHLORAMINE & TOTAL RESIDUAL CHLORINE
8. To the test sample from Step 7 above, add one *Chlorine DPD #3R
Tablet (6905A). Crush tablet with tablet crusher (0175). Cap the test
tube and mix until tablet disintegrates.
9. Insert the test tube into the Chlorine DPD Comparator, 0.1-1.0 ppm
(6978) or the Chlorine DPD Comparator, 1.0-6.0 ppm (6979).
Match sample color to a color standard. Record as Reading C. The
increase in color over Reading B is due to Dichloramine.
Reading C - Reading B = Dichloramine (ppm)
10. Reading C also represents the Total Residual Chlorine content.
PRO CE DURE 2:
FREE AVAIL ABLE CHLO RINE, COM BINED CHLO RINE
& TO TAL RE SID UAL CHLO RINE
FREE AVAILABLE CHLORINE
1. Follow Steps 1 through 4 under Procedure 1. This is Reading A.
Retain the test sample for the Combined Chlorine determination.
COMBINED CHLORINE & TOTAL RESIDUAL CHLORINE
2. To the test sample from Step 1 above, add one *Chlorine DPD #3R
Tablet (6905A). Crush tablet with tablet crusher (0175). Cap and
mix until tablet disintegrates.
3. Insert the test tube into the Chlorine DPD Comparator, 0.1-1.0 ppm
(6978) or the Chlorine DPD Comparator, 1.0-6.0 ppm (6979).
Match sample color to a color standard. Record as Reading C. Any
increase in color over Reading A is due to Combined Chlorine
(Monochloramine plus Dichloramine).
Reading C - Reading A = Combined Chlorine (ppm)
4. Reading C also represents the Total Residual Chlorine content.
PRO CE DURE 3:
TO TAL RE SID UAL CHLO RINE
The *Chlorine DPD #4R Tablet provides a one-step determination for total
residual chlorine and is used where it is not necessary to distinguish the
separate chlorine fractions.
1. Rinse the test tube (0898) with sample water. Fill to the bottom line
with sample water.
2. Add one *Chlorine DPD #4R Tablet (6899A). Crush tablet with
tablet crusher (0175). Cap and mix until tablet disintegrates.
3. Insert the test tube into the Chlorine DPD Comparator, 0.1-1.0 ppm
(6978) or the Chlorine DPD Comparator, 1.0-6.0 ppm (6979).
Match sample color to a color standard. Record as ppm Total Residual
Chlorine.
PRO CE DURE 4:
BRO MINE & IO DINE
Bromine and iodine may be determined by following the free available
chlorine test procedure and multiplying the comparator reading by the factors
given below.
1. Follow Steps 1 through 5 under Procedure 1.
2. To determine parts per million Bromine, multiply the comparator
reading from Step 1 by 2.2.
ppm Free Available Chlorine x 2.2 = ppm Bromine
3. To determine parts per million Iodine, multiply the comparator
reading from Step 1 by 3.5.
ppm Free Available Chlorine x 3.5 = ppm Iodine
PRO CE DURE 5:
pH
1. Rinse the test tube (0898) with sample water. Fill to the top line with
sample water.
2. Add one Phenol Red Tablet (6915). Crush tablet with tablet crusher
(0175). Cap and mix until tablet disintegrates.
3. Immediately insert tube into Phenol Red Comparator (2185). Match
sample color to a color standard. Record as pH.
LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Chal lenges
PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
®
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