
IRON, ALU MI NUM, & SUL FATE
WARNING! This set contains chemicals
that may be harmful if misused. Read
cautions on individual containers
carefully. Not to be used by children
except under adult supervision
SOIL TESTS
MODULE D
MODEL AM-32 · CODE 5938-01
QUANTITY CONTENTS CODE
30 mL *Ferric Iron Test Solution *5116PS-G
10 g *Iron Reagent Powder *5275-D
30 mL *Aluminum Test Solution *5101-G
30 mL *Sulfate Test Solution *5171-G
1 Pipet, transfer, plastic 0364
1 Test Tube, plastic, filtrate 0749
1 Spoon. 0.5 g 0698
1 Funnel, plastic 0459
1 Filter Paper, 50/pk 0465-H
1 Test Tube, glass 0242
2 Test Tubes, 1-8 mL, plastic, w/caps 0755
1 Spot Plate, double. plastic 0159
2 Stirring Rods, plastic 0519
1 Spoon, 0.05g, plastic 0696
1 Color Chart, Active Aluminum in Soil 1301
1 Color Chart, Sulfate in Soil 1314
1 Color Chart, Ferric Iron in Soil 1348
2 Pipets, plain, plastic, w/caps 0392
2 Pipets, plain, plastic 0352

*WARNING: Reagents marked with an * are considered to be potential
health hazards. To view or print a Material Safety Data Sheet (MSDS) for
these reagents go to www.lamotte.com. To obtain a printed copy, contact
LaMotte by e-mail, phone or fax..
To order individual reagents or test kit components, use the specified code
number.
EX TRAC TION PRO CE DURE
1. Fill a test tube (0755) to line 7 with *Universal Extracting Solution
(5173).
2. Use the 0.5 g spoon (0698) to add four level measures of soil to the
test tube. Cap and shake for one minute.
NOTE: When adding samples with high concentrations of
carbonates to *Universal Extracting Solution (5173), swirl tube to
mix for 30 seconds before capping to allow gases to escape.
3. Fold a piece of filter paper (0465) in half, then in half again. Press
corners together to form a cone. Place in funnel (0459). Pour
extract solution into funnel, collecting extract. This clear extract is
used for the test.
IRON TEST
1. Use a transfer pipet (0364) to add four drops of clear filtrate to a
large depression on a spot plate (0159).
2. Use the 0.05g spoon (0696) to add one level measure of *Iron
Reagent Powder (5275). Stir with a stirring rod (0519).
3. Use pipet (0392) to add one drop of *Ferric Iron Test Solution
(5116). Stir with a stirring rod (0519).
4. Match sample color to a color standard on the Ferric Iron in Soil
Color Chart (1348). Record as pounds per acre Ferric Iron.
IN TER PRE TA TION OF FER RIC IRON TEST
Most soils will contain iron in sufficient quantities to satisfy the needs of
most plants. A deficiency of iron is sometimes noted in alkaline soils,
and is indicated by a chlorotic condition of the plants. High tests may
be obtained on acid soils.

ALU MI NUM TEST
1. Use the transfer pipet (0364) to add two drops of clear filtrate to a
large depression on the spot plate (0159).
2. Use a plastic pipet (0352) to add two drops of *Universal
Extracting Solution (5173).
3. Use a second plastic pipet (0352) to add one drop of *Aluminum
Test Solution (5101). Stir with a stirring rod (0519). Wait one
minute.
4. Match sample color to a color standard on the Active Aluminum
Color Chart (1301).
IN TER PRE TA TION OF ALU MI NUM TEST
Aluminum occurs in large amounts in all soils in the form of
undecomposed minerals, and in the inorganic colloidal materials. In
neutral, slightly alkaline or slightly acid soils, the element is in inert
combinations that have no effect on plant growth. At greater degrees of
acidity, aluminum becomes active, capable of combining as soluble salts,
and thus exerting a toxic effect upon the growth of many plants,
especially those that are benefited by liming, when grown on acid soils.
A high, or very high, test is a certain index of an undesirable acid soil,
upon which acid-sensitive crops are almost certain to fail. A medium
test is not so serious, especially with grasses, corn, oats, potatoes and
tobacco. A low, or negative, test is desirable, except for distinctly
acid-tolerant plants.
SUL FATE TEST
1. Use the transfer pipet (0364) to add five drops of clear filtrate to
turbidity vial (0242).
2. Use capped pipet (0392) to add one drop of *Sulfate Test Solution
(5171). Gently swirl to mix.
3. Lay Sulfate Color Chart (1314) under neutral light. Hold turbidity
vial one-half inch above black strip in middle of chart. Look down
through turbid sample. Match sample turbidity to a turbidity
standard. Record as ppm Sulfate.
IN TER PRE TA TION OF SUL FATE TEST
Plants obtain the sulfur which they require from the sulfates in the soils.
Sulfates are usually present in the soil, although a deficiency may exist
in some localities. Unless present in excessive quantities, sulfates are not
harmful to plants. High tests may indicate recent addition of sulfur or
sulfate compounds to soil.

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PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
®
65938-D · 10.11