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HP-1
User Manual
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LaMotte HP-1 User Manual

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Page 1
HY DRO PONICS TEST KIT
WARNING! This set contains chemicals
that may be harmful if misused. Read
cautions on individual containers
carefully. Not to be used by children
except under adult supervision
MODEL HP-1 · CODE 3561
QUANTITY CONTENTS CODE
120 mL *Mixed Acid Reagent *V-6278-J 5 g *Nitrate Reducing Reagent *V-6279-C 60 mL *VM Phosphate Reagent *4410-H 30 mL *Wide Range Indicator *2218-G 30 mL *Sodium Hydroxide, 1.0N *4004WT-G 60 mL *Hydrochloric Acid, 1.0N *6130WT-H 15 mL *Sodium Hydroxide Reagent
*4259-E
Page 2
WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specified code number.
Read Demineralizer Bottle instruction manual before proceeding.
The Demineralizer Bottle (1152) will be the source of all deionized water used in these tests.
DI LU TION PRO CE DURE
If the test result in the nitrate, phosphate or potassium test is greater than the highest value it is necessary to perform a dilution. Use the 1.0 mL pipet (0354) and the 25 mL graduated cylinder (0417) as follows:
AMOUNT
OF SAMPLE
DILUTE
TO
MULTIPLY
RESULT BY
1.0 mL 5.0 mL 5
1.0 mL 10.0 mL 10
1.0 mL 20.0 mL 20
1.0 mL 25.0 mL 25
Use deionized water from the Demineralizer Bottle for all dilutions.
pH
A pH value of 7.0 indicates a neutral solution. Values above 7.0 indicate alkaline solutions, while values below pH 7.0 indicate acid solutions.
1. Fill a test tube (0106) to 5.0 mL line with sample solution.
2. Add 8 drops of *Wide Range Indicator (2218). Cap and mix.
3. Insert pH Wide Range Octa-Slide (3424) into Octa-Slide Viewer.
4. Insert test tube into the Octa-Slide Viewer (1100). Match sample
color to the pH Wide Range Octa-Slide Bar (3424). NOTE: If the pH of the sam ple is not be tween 6.2 and 6.8, it must be
ad justed to 6.5 be fore pro ceed ing the with the Ni trate, Phos pho rus, and Po tas sium tests. If the pH is be low 6.2 add *So dium Hy drox ide,
1.0N (4004), one drop at a time, pe ri od i cally check ing the pH un til it is brought into the range of 6.2 to 6.8. If the pH is above 6.8, add *Hy dro chlo ric Acid, 1.0N (6130) in the same man ner.
Page 3
NI TRATE NI TRO GEN
The range of the color standards in the Nitrate Nitrogen Color Bar is from
0.25 to 10 ppm Nitrate Nitrogen. If the nutrient solution is above this range, it should be diluted to bring the readings within the range. The dilution will depend upon the amount of nitrate in the solution. A 1 to 20 dilution is suggested.
NOTE: Place dispenser cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for refill reagents.
1. Fill a test tube (0106) to the 2.5 mL line with nutrient solution.
2. Add *Mixed Acid Reagent (V-6278) to the test tube to fill to the 5.0
mL line. Cap and mix. Wait two minutes.
3. Use the 0.1 g spoon (0699) to add one level measure (avoid any
excess) of *Nitrate Reducing Reagent (V-6279). Cap and invert the tube 50-60 times in one minute. Wait 10 minutes.
4. Insert Nitrate Octa-Slide Bar (3413) into Octa-Slide Viewer (1100).
5. Insert the tube into the Octa-Slide Viewer (1100). Match sample
color to a color standard. If the original sample was diluted, multiply the reading by 20 to obtain the final result in ppm Nitrate Nitrogen.
PHOS PHO RUS
The range of the color standards in the phosphorus comparator is from 3 to 30 ppm Phosphorus. Most nutrient solutions will have to be diluted to bring the reading within this range. A 1 to 2 dilution is suggested and involves diluting 5 mL of solution to 10 mL with deionized water.
1. Fill a test tube (0106) to the 5.0 mL line with nutrient sample.
2. Use the 1.0 mL pipet (0354) to add 1.0 mL of *VM Phosphate
Reagent (4410). Cap and mix. Wait 5 minutes.
3. Insert Phosphorous Octa-Slide Bar (3246) into Octa-Slide Viewer
(1100).
4. Insert test tube into the Octa-Slide Viewer (1100). Match sample
color to a color standard. If the original sample was diluted, multiply the reading by 2 to obtain the final result in ppm Phosphorus.
Page 4
PO TAS SIUM
A turbidime tric method is used for the determination of potassium in the nutrient solution. It is suggested that the nutrient solution be diluted to a 1 to 5 ratio with deionized water to bring the sample concentration within the range of the test. A 1 to 5 dilution involves diluting 2 mL of the sample to 10 mL with deionized water.
1. Remove the square inner tube and collar from the double tube
(0780).
2. Fill the round tube to the first line (8 mL) with nutrient sample.
3. Fill to the second line (16 mL total) with deionized water.
4. Add 2 drops of *Sodium Hydroxide Reagent (4259). Cap and mix.
5. Use the 0.5 g spoon (0696) to add one level measure of
Tetraphenylboron Powder (6364). Cap and shake vigorously until all the powder has dissolved. A white precipitate will form immediately. Wait 5 minutes.
6. Shake the tube again. Remove cap and slowly insert the square tube
with the collar. The square tube will slide up and down through the collar and fill with liquid.
7. Viewing from above, adjust the square tube into the turbid solution
until the black dot on its base is obscured. Hold the round tube at the top to avoid blocking light.
Record the height of the liquid in the square tube on the scale. Calibrations represent parts per million potassium. If the original sample has been diluted 1 to 5, the reading should be multiplied by 5 to obtain the potassium content of the original sample.
NOTE: Brush tubes thoroughly after each use.
LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Chal lenges
PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
SM
2.10
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