LaMotte DS-1-DC User Manual

DE TER GENT TEST KIT

WARNING! This set contains chemicals

that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision

MODEL DS-1-DC • CODE 4507-01

QUANTITY CONTENTS CODE

2 x 30 mL *DS Indicator Reagent *4508-G 15 mL DS Reference Solution 4513-E 50 g pH Adjustment Powder 4509-H 1 Test Tube, Test Sample w/cap 0282 1 Test Tube, Reference Sample w/cap 0283 1 Test Tube, 1-8 mL, plastic, w/cap 0755 1 Pipet, glass 0347 1 Spoon, 0.25 g, plastic 0695

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

To order individual reagents or test kit components, use the specified code number.

PRO CE DURE

STEP I - Determine if Detergent is Present

1. Use the calibrated test tube (0755) to measure 5 mL of the sample

solution. Add to the screw cap tube marked Test Sample (0282).

2. Use the 0.25 g spoon (0695) to add one measure of pH Adjustment

Powder (4509). Shake until dissolved.

3. Fill the pipet (0347) with *DS Indicator Reagent (4508) by squeezing

the rubber bulb, then inserting pipet into reagent. Add this amount of *DS Indicator Reagent to the Test Sample tube. Cap and shake for one minute.

4. Al low the tube to stand un til the two lay ers of the so lu tion sep a rate. The

wa ter layer will set tle to the bot tom and the re agent layer will rise to the top. Use chart be low to de ter mine if de ter gent is pres ent.

Bottom Layer Top Layer Quick Reading

Colorless Colored No Detergent in sample Some Color Some Color Some Detergent in sample Colored Colorless High Detergent in sample

NOTE: If the amount of detergent in the sample is to be determined, save this Test Sample and proceed to Step 2.

STEP 2 - Determine the Amount of Detergent Present

1. Use the calibrated test tube (0755) to measure 5 mL of detergent-free water.

Add to the screw cap tube marked Reference Sample (0283). (On field trips it may be necessary to carry a small supply of detergent free water.)

2. Use the 0.25 g spoon (0698) to add one measure of pH Adjustment Powder

(4509). Shake until dissolved.

3. Fill the pipet (0347) with *DS Indicator Reagent (4508) by squeezing the

rubber bulb, then inserting pipet into reagent. Add this amount of *DS Indicator Reagent to the Reference Sample tube.

4. Add one drop of DS Reference Solution (4513). Cap and shake for one

minute.

5. Allow the tube to stand until the two layers of solution separate. The color

produced in the bottom (water) layer is equivalent to 1 ppm of detergent.

6. Compare the color in the bottom layer of the Test Sample Tube from Part I

to the color of the bottom of the Reference Sample Tube.

If Test Sample Color Is: Test Sample Contains:

Lighter than Reference Same as Reference Darker than Reference

Less than 1.0 ppm Detergent

1.0 ppm Detergent More than 1.0 ppm Detergent

7. Add one drop of DS Reference Solution (4513) to the Reference Sample

Tube. Shake to mix. Compare the color as before. The color in the Reference Sample is now equal to 2.0 ppm. Continue this procedure, counting the number of drops of DS Reference Solution (4513) added, until the color of the bottom layer in each tube is the same. Each drop of the DS Reference Solution (4513) added to the Reference Sample Tube is equal to 1 ppm detergent in the sample.

NOTE: If at any time the top layer of the Test Sample or Reference Sample becomes colorless, add more DS Indicator Reagent (4508). The amount of this reagent added is not important as long as there is some color in the top layer.

LaMOTTE COM PANY

Helping Peo ple Solve An a lyt i cal Challenges

PO Box 329 • Chestertown • Mary land • 21620 • USA

800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394

Visit us on the web at www.lamotte.com

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