LaMotte DO 6 User Manual

DO 6

Instruction Manual

Economy Hand-held Dissolved Oxygen Meter

Code 5-0107

PREFACE

WARNING! This set contains chemicals

that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision

This manual serves to explain the use of the Dissolved Oxygen hand-held meter DO 6.

This manual functions in two ways: first as a step by step guide to help you operate the meter; second, it serves as a handy reference guide. This manual is written to cover as many anticipated applications of the DO 6 hand-held meter as possible. The information presented in this manual is subject to change without notice.

TABLE OF CONTENTS

1 Introduction.............................

2 Display & Keypad Functions ....................5

2.1 Display ................................ 5

2.2 Keypad ................................

3 Preparation ............................. 7

3.1 Inserting & Removing Rubber Boot ................. 7

3.2 Inserting the Batteries ........................ 8

3.3 Battery Replacement ......................... 8

3.4 Dissolved Oxygen Probe Information................. 9

3.5 Connecting the Probe to Meter ................... 10

3.6 Switching the Meter On....................... 11

3.7 Measurement Mode Selction:-Percentage Saturation (%),

Concentration (mg/L) (ppm) and Temperature (T) ........12

4 Calibration ............................ 13

4.1 Important Information on Meter Calibration ........... 13

4.2 Preparing the Meter for Calibration ................. 14

4.3 Temperature Calibration ...................... 15

4.4 Dissolved Oxygen Calibration in % Saturation ...........16

4.4.1 To calibrate 100% Saturation ................. 16

4.4.2 To calibrate 0% Saturation .................. 17

4.5 Dissolved Oxygen Calibration in mg/L or ppm Concentration

Mode................................ 18

4.5.1 To calibrate in mg/L (ppm) Concentration mode: ......18

5 Measurement ........................... 19

5.1 With Automatic Temperature Compensation (ATC) .......19

5.2 Manual Temperature Compensation ................ 20

5.3 Taking Measurements ........................ 21

5.3.1 To take measurements: .................... 21

5.4 Taking Pressure/Salinity Compensated DO Measurements .....21

5.4.1 Pressure Setting Adjustment ................. 22

5.4.2 Salinity Setting Adjustment.................. 23

6 Hold Function ...........................25

7 Advanced Setup Funtions ..................... 25

7.1 Advanced Setup Overview ..................... 25

7.1.1 To enter the % Saturation or Temperature Set Up menu: . . 25

7.1.2 To enter the mg/L (ppm) Concentration Set Up menu: . . . 26

7.2 (COF.1) Configuration Menu .................... 28

7.2.1 Selection of Automatic Temperature Compensation .....28

7.2.2 Selection of mg/L or ppm mode ................ 29

7.3 (CAL.2) Calibration Data...................... 30

7.3.1 Viewing the % Saturation calibration data ..........30

7.3.2 Viewing the mg/L (ppm) Concentration calibration data . . 30

7.4 (ELE.3) Electrode Properties .................... 30

7.4.1 Viewing the electrode Slope Factor .............. 31

7.4.2 Viewing the % Saturation Offset (only available in % Saturation

or Temperature Set up menu): ................... 31

7.4.3 Viewing the 100% Saturation mV value ........... 31

7.4.4 Viewing the 0% Saturation mV value ............ 31

7.5 (A

TO.4) Automatic Off ....................... 33

7.6 (

RST.5) Reset to Factory Default .................. 33

7.6.1 Calibration Reset ....................... 34

7.6.2 User Reset ........................... 35

7.7 (OFS.5) % Saturation Offset Adjustment.............. 36

7.8 (DP

R.7) Dissolved Oxygen Parameters ............... 37

7.8.1 Pressure Setting Adjustment ................. 37

7.8.2 Salinity Setting Adjustment ................. 39

8 Probe Care and Maintenance ................... 39

8.1 Probe Care .............................. 40

8.2 Pre-Membraned Caps Replacement .................40

8.3 To Replace the Probe Pre-Membraned Cap............. 40

8.4 Electrolyte Solution ......................... 41

9 Trouble-Shooting Guide ..................... 42

10 Error Messages ...........................43

11 Factory Default Settings ......................44

12 Specifications ...........................46

13 Accessories ............................ 48

14 Additional Information......................48

14.1 Dissolved Oxygen ......................... 48

14.1.1 General Information ..................... 48

14.1.2 Measurement Units ..................... 49

14.1.3 What is Being Measured ................... 49

14.1.4 Air Calibration........................ 50

14.1.5 Applications ......................... 51

15 Limited Warranty .........................51

16 Return of Items ..........................52

1 INTRODUCTION

CAL

Low Battery annunciator

Calibration Mode Annunciator

14-segment Liquid Crystal Display

Percentage (Percentage Saturation Mode)

parts per million

milligram per litre (Concentration Mode)

ATC annunciator

Temperature Mode

mg/L

ppm %

8.8.8.8

Thank you for purchasing the DO 6 Dissolved Oxygen palm-top meter. This economy microprocessor-based hand-held meter has a large custom LCD (Liquid Crystal Display) for clear and easy reading.

The DO 6 offers the measurement of dissolved oxygen in the percentage saturation mode and in the concentration mode. Temperature measurement is also available in degrees Celsius. The meter ensures accurate measurement of the Dissolved Oxygen values through its temperature, barometric pressure and salinity compensation features.

Your meter includes a dissolved oxygen probe, refill solution, a rubber boot, 4 alkaline “AAA” batteries, and instruction manual.

Please read this manual thoroughly before operating your meter.

To order other accessories and standard solutions, please refer to the Section on Accessories for more information.

2 DISPLAY & KEYPAD FUNCTIONS

2.1 Display

The meter has a large custom LCD that consists of 14-segment 4 digit display and operation annunciators for Percentage Saturation measurement mode (%), Concentration measurement mode (mg/L or ppm) and Temperature measurement mode in degrees Celsius (T). Other annunciators include “A” (when the ATC function is activated), “CAL” (when meter is in calibration mode) and battery diagram for low battery condition. See Figure 1.

Figure 1, Active LCD display for DO6

2.2 Keypad

OFF

CAL

HOLD

ENTER

MODE

The DO 6 meter has 6 keys on its splash-proof keypad; ON/OFF, HOLD/ENTER CAL, MODE,

p and q keys. Some buttons have several

functions depending on its mode of operation.

ON/OFF – Powers on and shuts off the meter. The meter will start in the measurement mode it was in when last switched off.

CAL – Activates the calibration mode for Percentage Saturation, Concentration and Temperature calibrations.

Deactivates calibration or setup without confirming a value in calibration mode.

HOLD -Activates/Deactivates freezing of the measured reading while in measurement mode.

ENTER - Confirms the calibration values in Calibration mode and the selections in the SETUP menu.

pq – Sets the calibration values during the Concentration and Temperature calibration.

Scrolls through each SETUP and its sub group menu. Set offset adjustments and configuration settings.

MODE - Selects the measurement option between DO Percentage Saturation measurement; DO Concentration measurement and Temperature measurement.

When pressed together with ON/OFF key during power on, SETUP mode is selected. This menu allows meter customization with preferences such as activating ATC selection, DO concentration measurement unit selection, viewing of the last calibration data, viewing of the electrode properties, selecting the auto power off, resetting calibration data or meter settings back to factory default, setting the offset adjustments, setting barometric pressure in Hg or PA for barometric pressure compensation and setting of the salinity value for salinity compensation.

3 PREPARATION

3.1 Inserting & Removing Rubber Boot

1. To remove meter from rubber boot, push out from the bottom edges of

meter until it is completely out of boot. Ensure that the cables of Dissolved Oxygen electrode or temperature probe are not connected

2.. To insert meter into rubber boot, slide in from the top of meter before

pushing the bottom edges of meter down to set it into position. Lift up the stand at the back of meter for bench top applications if necessary.

Figure 2: Inserting or removing the rubber boot

3.2 Inserting the Batteries

3.0

The battery compartment is found at the back of instrument as shown in Figure 3. To open the battery compartment first remove protective rubber boot/stand then:

1. Push in the direction of arrow and lift up the cover.

2. Note the polarity of battery before inserting into position.

3. After replacement, place cover back and press down until it locks tight.

Figure 3: Inserting the batteries

3.3 Battery Replacement

A low battery annunciator in the LCD alerts you when battery power is running low. See Figure 4. Replace with the same type as recommended by the manufacturer.

Figure 4: Low Battery Condition

Caution: Power off the meter when changing battery.

3.4 Dissolved Oxygen Probe Information

16 mm

12 mm

Detachable Pre-membraned Cap (Probe Sensing Area)

Figure 5: 12 mm Galvanic DO probe

The DO 6 hand-held meter is supplied with a Dissolved Oxygen Probe that works on galvanic principle, that is, it does not require any polarising voltage from your meter. Rather it will generate a millivolt signal proportional to the amount of oxygen in the solution.

Your DO Probe has a twin cable, one with a BNC connector for the DO measurement input, and the other with a phono jack plug for the temperature measurement input. Its sensing area consists of a cathode, anode, and an inner electrolyte which is separated from your sample solution by an oxygen permeable membrane pre-membraned to the probe’s detachable cap. Reconditioning of your probe is made easy with the introduction of this specially designed detachable pre-membraned cap.

This light weight probe has a built-in temperature sensor for Automatic Temperature Compensation (ATC) with 12mm diameter epoxy body housing and 16 mm diameter Noryl detachable cap. Its compact sensing area reduces air entrapment which makes it easy to obtain fast, accurate and stable readings. Simply stir the probe in the solution being measured. Shaking will aid to remove bubbles if needed before taking a reading. Also, always ensure to remove water drops from the membrane when calibrating in air.

Proper use of probe is essential to ensure that the optimum measurement is taken in a short time. Always immerse the probe beyond the pre-membraned cap. The minimum water flow rate is 2 inch/second across membrane. The temperature range for use is within 0 to 50 °C.

Always ensure that the probe’s membrane is protected against any scratches or dents. The whole pre-membraned cap has to be replaced if the membranedamaged. It is also important to keep the membrane clean so as to produce an optimum and accurate measurement.

See Section 8 - "Probe Care and Maintenance" for more information.

3.5 Connecting the Probe to Meter

BNC Connector for DO probe

Phono jack for Temperatureprobe

1. Insert the BNC connector from the electrode to the BNC connector socket

on the meter accordingly and rotate connector clockwise until it locks. See Figure 6 below.

2. To remove, simply rotate the connector in counter-clockwise direction

until it unlocks, and slide the connector off the socket.

3. Plug the phono jack of temperature sensor into the phono socket of the

meter as shown in Figure 6 below.

Caution: Do not pull or force the probe cord or the probe wires might disconnect.

Note: Keep connectors clean. Do not touch connector with soiled hands.

Figure 6: DO 6 probe to meter connections

3.6 Switching the Meter On

CAL 13 CAL 24

TAv g

1.00

10.00

D06

mg/L NTU

mg/L

ppm

%pH

8.8.8.8

OFF

When switching the meter on, it will go through a series of display, eventually showing the revision number of the meter before going to the measurement mode. The mode of measurement which the meter displays will be similar to the last measurement mode to which the meter is switched off.

Press ON/OFF key to power up your meter.

1. All LCD segments will light up

for 1.5 seconds.

2. The display will then

momentarily show the meter’s identification “DO 6” with the annunciators for percentage saturation mode and concentration mode.

3. The display continues to switch

to display meter’s revision number for 1.5 seconds before finally showing the measurement mode.

4. Either percentage saturation

measurement mode, concentration measurement mode or temperature measurement mode will be displayed following the previous measurement mode to which the meter is switched off.

Figure7: Power Up sequence

3.7 Measurement Mode Selection:- Percentage Saturation (%),

10.00

8.24

25.0

MODE

mg/L

Automatic Temperature Compensation

Percentage Saturation

Concentration Mode

Temperature Mode

Concentration (mg/L)(ppm) and Temperature(T)

By simply pressing the MODE key, you can select the measurement mode of percentage saturation measurement, concentration measurement or temperature measurement.

The customized annunciator shall indicate the selection of the measurement mode.

Figure 8: Measurement mode selection using the mode key

4 CALIBRATION

4.1 Important Information on Meter Calibration

Your meter has three measurement modes namely the DO % saturation measurement mode, the DO mg/L (ppm) concentration mode and the Temperature measurement mode.

Since the mg/L (ppm) concentration value of dissolved oxygen varies with temperature, barometric pressure and salinity, the meter with its electrode has to be calibrated with consideration to these factors to ensure a proper measurement. Therefore, it is very important to set the proper temperature, barometric pressure and salinity values in the meter prior to any calibration or measurement process. Since the dissolved oxygen measurements in % saturation will linearly affect the mg/L (ppm) concentration measurement, it is important that the meter has to be first calibrated in the % Saturation mode and temperature mode before commencing the mg/L (ppm) concentration mode calibration.

In % saturation calibration mode, the dissolved oxygen calibration values will vary according to the barometric pressure correction set in the meter. Therefore it is necessary to set the barometric pressure first before proceeding to % Saturation calibration. The following table is an example which explains the acceptance calibration values when % Saturation mode calibration is attempted under different absolute value and under two different barometric pressures.

% Saturation factory default value

less than 10% 0% 0%

10.1% to 49.9% Err.1 (error 1) Err.1 (error 1)

50% to 200% 100% 92.1%

Acceptance Calibration Value (760mmHg)

In the mg/L (ppm) concentration calibration mode, barometric pressure, salinity and temperature of the calibration sample needs to be set in the meter prior to calibration. Since the pressure has already been set in the % saturation calibration which is done earlier and assuming that the calibration is done on the same barometric pressure and if you are using the temperature sensor in automatic temperature compensation mode, you only need to input the known salinity of the calibration sample to the meter. The dissolved oxygen mg/L (ppm) concentration value can then be calibrated to the known sample concentration calibration value. Note that the concentration calibration

window is +/- 40% of the factory default value. However the minimum point of calibration is 2 mg/L.

When you recalibrate your meter in % saturation mode, old calibrations in % saturation will be replaced while the % saturation offset adjustment will be erased. However, recalibration in the mg/L (ppm) concentration mode will only replace the old calibration in the concentration mode and do not affect the calibration in the percentage saturation mode.

To completely recalibrate your meter, or when you use a replacement electrode, it is best to clear all calibration data. To erase all the old calibration data completely, see Section 7.6 (rSt.5) Reset to Factory Default.

4.2 Preparing the Meter for Calibration

Before starting calibration, make sure you are in the correct measurement mode and in the correct calibration sequence. The temperature and the % Saturation calibration must be done first before attempting to do the mg/L (ppm) Concentration calibration.

In % Saturation, the meter is able to perform either a one point calibration or a 2 point calibration. For one point calibration, it is recommended that you perform a 100% Saturation calibration in saturated air. If you opt for 2 point calibration, you can calibrate for 100% Saturation in saturated air and 0% Saturation using a zero oxygen solution. The meter will take several minutes to reach 0% Saturation value after submersion into the zero solution.

Rinse the probe well in the de-ionized (DI) water or rinse solution and wipe the probe carefully taking care of the membrane.

Calibrate the meter in all the modes to ensure the highest accuracy throughout the DO measurement range. In % Saturation calibration, should there be a calibration failure for 0% Saturation slope calibration; the meter may have exceeded the limit of 10% of the factory calibrated absolute value. The sensor may have to be re- conditioned. Also, note that the DO 6 meter will not perform 0% or 100% Saturation calibration for absolute value ranging from

10.1% to 49.9%. The meter also will not perform the mg/L (ppm) Concentration calibration for an absolute value of less than 2.00 mg/L (ppm) or calibration which is out of its window tolerance of +/- 40% from the factory default value. An error message of “Err.1” will be indicated and will return the meter to the measurement mode.

All new calibration values will automatically override the existing data. It is recommended to calibrate the meter periodically and or if it is suspected to be inaccurate.

Always rinse the probe with either DI water or rinse solution before and after

CAL

HOLD

ENTER

24.3

25.0

“CO” will display for

1.5 seconds

24.3

CAL

25.0

CAL

Factory default Temperature Measurement

Figure 9: Temperature Calibration Sequence

each calibration/sample measurement. When calibrating in air, make sure that any water droplets from the probe’s membrane are removed.

For details please refer to Section 8 - PROBE CARE AND MAINTENANCE. Note: The DO 6 factory calibrated default value is in respect to 760mm Hg or

101.3 kPa barometric pressure (sea level). To set the barometric pressure to different value, see Section 5.4.1 Pressure Setting Adjustment.

4.3 Temperature Calibration

DO mg/L (ppm) Concentration measurement is dependent on the temperature of the sample to be measured. Most users will choose to have the DO 6 automatically compensate for temperature using the temperature sensor that is built into the DO probe. However, it is possible to manually enter a known temperature to the meter (manual temperature compensation). The built-in temperature sensor of the DO 6 probe is factory calibrated. However, the temperature can be re-calibrated if needed and is recommended when a replacement probe is used.

Before calibration, ensure that the meter displays annunciator “A” to indicate that it is in automatic temperature compensation mode. Please see Set Up menu on page 36 for its activation procedure.

1. Switch the meter on. Press

MODE to select temperature mode. The display should show the annunciator “T” for temperature mode and“A” for automatic temperature compensation mode.

2. Dip the probe to a solution of known temperature (i.e. a temperature

CAL

HOLD

ENTER

83.0

CAL

100.0

“CO” will display for

1.5 seconds

A value with respect to the factory default calibration will be displayed

100.0

85.2

CAL

Calibration point will be displayed for 1.5 seconds

Figure 10: 100% Saturation Calibration

bath).Allow some time for the reading to stabilize.

3. Press the CAL key. The CAL indicator will appear and blinks above the

display. The temperature value displayed is with respect to the factory default calibration.

4. Press the p andq keys to adjust the meter reading to the correct

temperature value (i.e. the temperature of the temperature bath)

5. Press the HOLD/ENTER key to confirm temperature calibration and

return to measurement mode.

NOTE: To exit from Temperature Calibration mode without confirming the calibration, press CAL instead of HOLD/ENTER. Temperature calibration is restricted to ± 5°C from the factory default temperature measurement displayed during calibration.

4.4 Dissolved Oxygen Calibration in % Saturation

You can calibrate this meter quickly and easily in air. The exact calibration value depends on barometric pressure. The meter is set to a factory default of 760 mm Hg, which results in a calibration value of 100% Saturation in air.

NOTE: If the barometric pressure setting has been changed from 760 mm Hg, the calibration value in air will automatically adjust to a value other than 100%. The adjusted value will be correct for the new barometric pressure setting.

See Section 5.4.1 Pressure Setting Adjustment on page 26 to change the pressure setting.

4.4.1 To calibrate 100% Saturation:

1. Rinse the probe well with DI water or

rinse solution. Do not touch the membrane.

2. Press the MODE key to select the %

Saturation mode.

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