*WARNING: Reagents marked with an * are considered to be potential health
hazards.
www.lamotte.com.
reagent label, in the contents
that follows or precedes the four digit code number. For example if code is
4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
To order individual reagents or test kit components, use the specified code
number.
To view or print a Safety Data Sheet (SDS) for these reagents go to
Search the four digit reagent code number listed on the
list or in the test procedures. Omit any letter
Accessories
DESCRIPTIONCODEDESCRIPTIONCODE
Test Tubes, with Caps0290-6USB Wall Adapter1721
Replacement Chamber 3-0038Car Charger5-0132
USB Cable1720-01
WARNING: Only use the USB Cable (1720-01) that is supplied with the kit.
Make no substitutions.
Ozone is sometimes used in place of, or in conjunction with, chlorine or other
halogens for disinfection of pool, spa or drinking waters. Recently, large aquatic
facilities have begun using ozone as a disinfectant in many artificial habitats.
APPLICATION
Drinking, pool, and aquatic waters.
RANGE
0.00 - 0.40 ppm Ozone
METHOD
Ozone rapidly and stoichiometrically decolorizes Indigo Trisulfonate under
acidic conditions.
HANDLING & PRESERVATION
Ozone is extremely unstable in aqueous solutions. Test must be performed
immediately and the sample must not be agitated.
1. Use the 50 mL graduated
cylinder (0418) to carefully add
45 mL of Ozone Buffer (3991) to
amber glass bottle marked HR
Reagent (3988-MT-G).
2. Use the 5 mL volumetric pipet
(0329) and pipette pump
(30527) to add 5 mL of Indigo
Blue Stock Solution (3989) to
the amber glass bottle. Cap and
mix.
Analysis
3. Use the 1.0 mL transfer pipet
(2-2170) and pipette
(30527) to add 1.0 mL of HR
Reagent (3988) to each of 2
clean colorimeter tubes (0290).
4. If chlorine is present, add 3
drops Chlorine Inhibitor (3990)
to each tube. Cap tubes.
5. Take one of the prepared
colorimeter tubes and sampling
apparatus (0681) to sampling
site.
6. Lower the end of the tubing of
sampling apparatus to desired
depth. Slowly withdraw and
depress plunger several times to
purge syringe and tubing. Slowly
Analysis
withdraw plunger to fill purged
syringe.
7. Remove plastic tubing from
syringe. Remove cap from the
prepared tube. Place tip of
syringe against inside of the
prepared tube. Slowly depress
plunger and fill to the 10 mL
line and cap. This is the Sample
Tube. NOTE: Do not shake or
invert the sample.
8. Fill the second prepared tube
(0967) to the 10 mL
ozone free water. This is the
Reagent Blank.
11. Scroll to and select 071 Ozone
IB LR from menu.
12. Insert the Reagent Blank tube
into chamber, close lid and
select Scan Blank.
071 Ozone IB LR
Analysis
Scan Blank
Scan Sample
12:00:00 001/500
13. Insert reacted sample tube into
chamber. Close lid. Select Scan Sample. Record result as Ozone.
NOTE: The quality of the Indigo Blue Stock Solution (3989) supplied will
prepare five batches of HR Reagent.
The HR Reagent must be made fresh each week. If reagent is refrigerated, it
may be kept up to 3 weeks.
NOTE: Zeroing the meter with sample water or an empty chamber will result in
an error when reading reacted samples. Meter must be zeroed with a reagent
blank.
NOTE: The meter will remember the last scanned blank reading. It is not
necessary to scan a blank each time the test is performed. To use the
previous blank reading, instead of scanning a new one, scroll to Scan Sample
and proceed. For the most accurate results, the meter should be blanked
before each test and the same tube should be used for the blank and the
reacted sample.
The default setting for the data logger is enabled. The meter will log the last
500 data points. The counter in the center bottom of the display will show how
many data points have been logged. The display will show 500+ when the data
logger has exceeded 500 points and the data points are being overwritten.
Calibration
1. From the Editing Menu, press
or to scroll to
Logging.
2. Press
ENTER
to select Logging.
3. Press or to scroll to
desired function.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
Logging
Display Test Log
Logging Enabled
Logging Disabled
Erase Log
12:00:00 001/500
Logging
Display Test Log
Logging Enabled
Logging Disabled
Erase Log
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about 1
second and return to the EditingMenu.
The Factory Setup menu is used in manufacturing of the colorimeter. This
menu is not for use by the operator in the field.
SETTING POWER SAVE
The power saving Auto Shutoff feature will turn the meter off when a button
has not been pushed for a set amount of time. The default setting is disabled.
To change the setting:
1. From the Editing Menu, press
or to scroll to Set
Power Save.
2. Press
ENTER
to select Set Power
Save.
3. Press or to scroll to
desired function.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
Set Power Save
Disable
5 Minutes
15 Minutes
30 Minutes
12:00:00 001/500
Set Power Save
Disable
5 Minutes
15 Minutes
30 Minutes
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing.... for about 1
second and the meter will retun
to the Editing Menu.
The backlight illuminates the display for enhanced viewing. The default setting
is 10 seconds. If Button Control is chosen the backlight button on the key pad
will act as an on/off switch and the backlight will remain on or off when the
meter is being used. When one of the other settings – 10, 20 or 30 seconds –
is chosen, the display will be illuminated for the specified amount of time after
any button is pressed.
NOTE: The backlight feature uses a significant amount of power. The longer
the backlight is on, the more frequently the battery will have to be charged if
the USB/Wall Adapter is not being used.
1. From the Editing Menu, press
or to scroll to
Backlight Time.
2. Press
ENTER
to select Set
Backlight Time.
3. Press or to scroll to
desired option.
Editing Menu
Logging
Factory Setup
Set Power Save
Set Backlight Time
12:00:00 001/500
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about 1
second and the meter will return
to the Editing Menu.
There are seven languages available: English, Spanish, French,
Portuguese, Italian, Chinese, and Japanese.
1. From the Editing Menu, press
or to scroll to
Select Language.
2. Press
ENTER
to select Select
Language.
3. Press or to scroll to
desired language.
Editing Menu
Factory Setup
Set Power Save
Set Backlight Time
Select Language
12:00:00 001/500
Select Language
English
Spanish
French
Portugese
12:00:00 001/500
Select Language
English
Spanish
French
Portugese
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about 1
second and the meter will return
to the Editing Menu.
Set Power Save
Set Backlight Time
Editing Menu
Bluetooth Menu
Select Language
12:00:00 001/500
NOTE: If meter unintentionally switches to another language, use the
procedure above to reset the meter to the desired language. For example, to
reset the meter to English:
Long menus, such as the Tsting Menu, incorporate a looping feature which
allows the user to quickly reach the last choice in the menu from the first
choice. In a looping menu the last choices in the menu are above the first
choice and scrolling upward moves through the menu in reverse order.
Scrolling downward moves through the menu from first choice to last but
the menu starts over following the last choice. So all menu choices can be
reached by scrolling in either direction. The diagrams below demonstrate a
looping menu.
The meter may be interfaced with any Windows-based 64-bit computer
by using the LaMotte WaterLink Connect 2 program and a USB cable. The
program will store test information and results in a local database and allow
for exporting this data to a comma separated value (CSV) file. The meter
will send the following data: time/date stamp, name of test, sample value,
sample units, meter name, and location. To download WaterLink Connect 2
go to http:// softwarecenter.lamotte.com/. Select “WaterLink Connect 2 FREE
Download”.
BATTERY
BATTERY/AC OPERATION
The colorimeter may be operated on battery power using a USB wall adapter
or USB computer connection. If using the meter as a bench top unit, use the
wall adapter if possible to extend the battery life. The meter will remain on
when the USB adapter is used.
To charge the lithium ion battery with the wall adapter, plug the smaller end of
the USB cable (USB mini B connector) into the meter and the larger end of the
USB cable (USB type A connector) into the wall adapter. Plug the wall adapter
into an AC outlet. Reinsert the rubber USB port plug after charging. Failure
to insert the USB port plug when the meter is not connected to a computer
by USB or actively charging by USB could result in damage to internal
components.
To charge the battery from a computer, plug the smaller end of the USB cable
(USB mini B connector) into the meter and the larger end of the USB cable
(USB Type A connector) into a USB port on a computer. Reinsert the USB port
plug after charging. Failure to insert the USB port plug when the meter is not
connected to a computer by USB or actively charging by USB could result in
damage to internal components.
The battery icon will show no bars and flash when the unit first turns on. Then
the indicator will indicate the battery status by showing 0, 1, 2, 3, or 4 bars.
It will take 6 hours to fully charge a low battery. The battery icon will flash
when the battery is charging. The battery icon will show four bars and stop
flashing when it is fully charged. The charging circuit will automatically switch
to a float charge when the battery is fully charged. The charger may remain
connected. Some computers will NOT supply power to their USB ports during
standby operation. The wall adapter will charge the unit continuously. Storing
the meter above ambient room temperature will decrease the battery charge
more quickly than storage at room temperature. If the meter does not turn
on, it means that the battery is at a very low charge. Charging the battery with
the wall adapter in this state may take up to 10 hours. At low temperatures,
approaching 0 °C, the battery will charge more slowly. It will not charge at all
below 0 °C.
The battery icon will show no bars and continuously flash if the battery is
getting low but the unit will still operate normally. A “Low Battery” message
on the status bar of the display will replace the time when the battery voltage
is too low for proper operation and accuracy may be degraded. A “Shutdown
Low Batt” message on the display will appear for a few seconds before the
power is switched off when the battery is too low to operate the unit. When
the battery icon simultaneously flashes bars 1 and 2 followed by bars 3 and 4,
it is an indication that the battery is damaged and technical support should
be contacted.
To extend the life of the battery:
• Shut down the unit with the power switch when not taking
measurements or use the power save option to have the unit
automatically turn off after 5 minutes.
• Store the unit at a moderate temperature.
• Fully charge the battery before storing the unit for extended periods
of time.
• Fully charge the battery at least once per year. Failure to do so may
result in a permanently drained battery.
• Limit backlight use. The unit consumes three times the normal power
when the backlight is on. Set the backlight time option to 10 seconds
or select “Button Control” and keep the backlight off.
BATTERY REPLACEMENT
The lithium ion battery used in this unit should last for many years with
normal use. When it no longer powers the unit long enough to meet testing
requirements it will need to be replaced. Lithium ion batteries that are
properly charged and stored do not usually lose all capacity; they just have
less capacity after hundreds of charge cycles. This unit uses a custom battery
assembly that is only available from LaMotte Company. Battery replacement
must be performed at a LaMotte authorized repair facility. The water resistant
housing of this meter should not be opened by the user. Contact LaMotte
Company by phone (1-800-344-3100) or email (tech@lamotte.com) for a
return authorization number.
MAINTENANCE
CLEANING
Clean the exterior housing with a damp, lint-free cloth. Do not allow water
to enter the light chamber or any other parts of the meter. To clean the
light chamber and optics area, point a can of compressed air into the light
chamber and blow the pressurized air into the light chamber. Use a cotton
swab dampened with Windex® window cleaner to gently swab the interior of
the chamber. Do not use alcohol; it will leave a thin residue over the optics
when dry.
Should it be necessary to return the meter for repair or servicing, pack the
meter carefully in a suitable container with adequate packing material. A
return authorization number must be obtained from LaMotte Company by
calling 800-344-3100 (US only) or 410-778-3100, faxing 410-778-6394,
or emailing tech@lamotte.com. Often a problem can be resolved over the
phone or by email. If a return of the meter is necessary, attach a letter with
the return authorization number, meter serial number, a brief description of
problem and contact information including phone and FAX numbers to the
shipping carton. This information will enable the service department to make
the required repairs more efficiently.
Waste Electrical and Electronic Equipment (WEEE)
Natural resources were used in the production of this equipment. This
equipment may contain materials that are hazardous to health and the
environment. To avoid harm to the environment and natural resources, the
use of appropriate take-back systems is recommended. The crossed out
wheeled bin symbol on the meter encourages the use of these systems when
disposing of this equipment.
Take-back systems will allow the materials to be reused or recycled in a
way that will not harm the environment. For more information on approved
collection, reuse, and recycling systems contact local or regional waste
administration or recycling services.
GENERAL OPERATING INFORMATION
OVERVIEW
The meter is a portable, microprocessor controlled, direct reading colorimeter.
It has a graphical liquid crystal display and 6 button keypad. These allow the
user to select options from the menu driven software, to directly read test
results or to review stored results of previous tests in the data logger. The
menus can be displayed in seven different languages.
The LaMotte tests are precalibrated for LaMotte reagent systems.
The colorimeter displays the result of these tests directly in units of
concentration.
The optics feature a colored LED. The LED has a corresponding silicon
photoiode with an integrated interference filter. The interference filter selects
a narrow band of light from the corresponding LED for the colorimetric
measurements.
A USB wall adapter, USB computer connection or lithium battery powers the
colorimeter.
GENERAL OPERATING INFORMATION
The operation of the colorimeter is controlled by the menu driven software
and user interface. A menu is a list of choices. This allows a selection of
various tasks for the colorieter to perform, such as scan blank and scan
sample. The keypad is used to make menu selections that are viewed on the
display.
The keypad has 6 buttons which are used to perform specific tasks.
This button will scroll up through a list of menu selections.
ENTER
The button is used to select choices in a menu viewed on the
display.
This button controls the backlight on the display.
This button will scroll down through a list of menu selections.
EXIT
This button exits to the previous menu.
This button turns the meter on or off.
ENTER
EX I T
THE DISPLAY AND MENUS
The display allows menu selections to be viewed and selected. These
selections instruct the colorimeter to perform specific tasks. The menus are
viewed in the display using two general formats that are followed from one
menu to the next. Each menu is a list of choices or selections.
The display has a header line at the top and a footer line at the bottom. The
header displays the title of the current menu. The footer line displays the
time and the date, the data logger status and the battery status. The menu
selection window is in the middle of the display between the header and the
footer.
The menu selection window displays information in two general formats. In
the first format only menu selections are displayed. Up to 4 lines of menu
selections may be displayed. If more selections are available they can be
viewed by pressing the arrow buttons to scroll the other menu
selections into the menu selection window. Think of the menu selections as
a vertical list in the display that moves up or down each time an arrow button
is pressed. Some menus in the colorimeter are looping menus.
The top and bottom menu choices are connected in a loop. Scrolling down
past the bottom of the menu will lead to the top of the menu. Scrolling up
past the top of the menu will lead to the bottom of the menu.
A light bar will indicate the menu choice. As the menu is scrolled through, the
light bar will highlight different menu choices. Pressing the
select the menu choice that is indicated by the light bar.
In the second format the menu choice window takes advantage of the
graphical capabilities of the display. Large format graphic information, such
as test results or error messages or the LaMotte logo is displayed. The top
two lines of the display are used to display information in a large, easy to read
format. The menus work in the same way as previously described but two lines
of the menu are visible at the bottom of the display.
HeaderMenu Title
Message or Result Window
ENTER
button will
Result or
Message
Another
Main Window Selection And Another
Footer
As described previously, the
current menu and a return to the previous menu. This allows a rapid exit from
an inner menu to the main menu by repeatedly pushing the
Pushing at any time will turn the colorimeter off.
More choices are available and can be viewed by scrolling
up and/or down through the display.
HeaderIdentifies the current menu and information on units and
reagent systems if applicable.
FooterIn the data logging mode the number of the data point
is displayed and the total number of data points in the
memory will be shown. The footer also shows current time
and battery status
TUBES AND CHAMBERS
The colorimeter uses one type of tube (Code 0290) for all test factors.
The handling of the tubes is of utmost importance. Tubes must be clean and
free from lint, fingerprints, dried spills and significant scratches, especially
the central zone between the bottom and the sample line.
Scratches, fingerprints and water droplets on the tube can cause stray light
interference leading to inaccurate results. Tubes that have been scratched in
the light zone through excessive use should be discarded and replaced with
new ones.
Tubes should always be washed on the inside and outside with mild detergent
prior to use to remove dirt or fingerprints. The tubes should be allowed to airdry in an inverted position to prevent dust from entering the tubes. Dry tubes
should be stored with the caps on to prevent contamination.
After a tube has been filled and capped, it should be held by the cap and the
outside surface should be wiped with a clean, lint-free absorbent cloth until it
is dry and smudge-free. Handling the tube only by the cap will avoid problems
from fingerprints. Always set the clean tube aside on a clean surface that will
not contaminate the tube. It is imperative that the tubes and light chamber be
clean and dry. The outside of the tubes should be dried with a clean, lint-free
cloth or disposable wipe before they are placed in the meter chamber.
Tubes should be emptied and cleaned as soon as possible after reading a
sample to prevent deposition of particulates on the inside of the tubes.
Variability in the geometry of the glassware and technique is the predominate
cause of variability in results. Slight variations in wall thickness and the
diameter of the tubes may lead to slight variations in the test results. To
eliminate this error the tubes should be placed in the chamber with the same
orientation each time.
Chambers which have been scratched through excessive use should be
discarded and replaced with a new one.
If a test result is out of the range of the meter, it must be diluted. The test
should then be repeated on the diluted sample. The following table gives quick
reference guidelines for dilutions of various proportions.
Amount of SampleDeionized Water to Bring
Final Volume to 10 mL
10 mL0 mL1
5 mL5 mL2
2.5 mL7.5 mL4
1 mL9 mL10
0.5 mL9.5 mL20
All dilutions are based on a final volume of 10 mL, so several dilutions will
require small volumes of the water sample. Graduated pipets should be
used for all dilutions. If volumetric glassware is not available, dilutions can
be made with the colorimeter tube. Fill the tube to the 10 mL line with the
sample and then transfer it to another container. Add 10 mL volumes of
deionized water to the container and mix. Transfer 10 mL of the diluted
sample to the colorimeter tube and follow the test procedure. Repeat the
dilution and testing procedures until the result falls within the range of the
calibration. Multiply the test result by the dilution factor. For example, if 10 mL
of the sample water is diluted with three 10 mL volumes of deionized water,
the dilution factor is four. The test result of the diluted sample should be
multiplied by four.
Multiplication Factor
GENERAL INFORMATION
PACKAGING AND DELIVERY
Experienced packaging personnel at LaMotte Company assure adequate
protection against normal hazards encountered in transportation of
shipments.
After the product leaves LaMotte Company, all responsibility for safe delivery
is assured by the transportation company. Damage claims must be filed
immediately with the transportation company to receive compensation for
damaged goods.
GENERAL PRECAUTIONS
READ THE INSTRUCTION MANUAL BEFORE ATTEMPTING TO SET UP OR
OPERATE THE METER. Failure to do so could result in personal injury or
damage to the meter. The meter should not be used or stored in a wet or
corrosive environment. Care should be taken to prevent water from wet tubes
from entering the meter chamber.
Read the labels on all LaMotte reagent containers prior to use. Some
containers include precautionary notices and first aid information. Certain
reagents are considered hazardous substances and are designated with
a * in the instruction manual. Safety Data Sheets (SDS) can be found at
www.lamotte.com. Read the SDS before using these reagents. Additional
emergency information for all LaMotte reagents is available 24 hours a day
from the National Poison Control Center 1-800-222-1222 or by contacting
the 24 hour emergency line for ChemTel 1-800-255-3924 (USA, Canada,
Puerto Rico); locations outside the North American Continent 813-2480585 (call collect). Be prepared to supply the name and four-digit LaMotte
code number found on the container label or at the top of the SDS or in
the contents list of the procedure. LaMotte reagents are registered with a
computerized poison control information system available to all local poison
control centers.
Keep equipment and reagent chemicals out of the reach of young children.
Ensure that the protection provided by this equipment is not impaired. Do not
intall or use this equipment in a manner that is not indicated in this manual.
LIMITS OF LIABILITY
Under no circumstances shall LaMotte Company be liable for loss of life,
property, profits, or other damages incurred through the use or misuse of its
products.
The colorimeter has been independently tested and has earned the European
CE Mark of compliance for electromagnetic compatibility and safety. To view
certificates of compliance, go to the LaMotte website at www.lamotte.com.
IP67 Certification
The meets IP67 standards for protection against dust and immersion only
when the USB port plug is in place. Documentation is available at www.
lamotte.com.
WARRANTY
LaMotte Company warrants this instrument to be free of defects in parts
and workmanship for 2 years from the date of shipment. If it should become
necessary to return the instrument for service during or beyond the warranty
period, contact our Technical Service Department at 1-800-344-3100 for a
return authorization number or visit www.lamotte.com for troubleshooting
help. The sender is responsible for shipping charges, freight, insurance and
proper packaging to prevent damage in transit. This warranty does not
apply to defects resulting from action of the user such as misuse, improper
wiring, operation outside of specification, improper maintenance or repair,
or unauthorized modification. LaMotte Company specifically disclaims any
implied warranties or merchantability or fitness for a specific purpose and
will not be liable for any direct, indirect, incidental or consequential damages.
LaMotte Company’s total liability is limited to repair or replacement of the
product. The warranty set forth above is inclusive and no other warranty,
whether written or oral, is expressed or implied. Keep the proof of purchase
for warranty verification.
If the message OVERRANGE is displayed when scanning a sample, the
sample may be over range or under range. If the sample is over range, the
sample should be diluted and tested again (see Sample Dilution Techniques
and Volumetric Measurements, page 22).
If overrange is displayed, scroll to
Calibrate and press
ENTER
to view
071 Ozone IB LR
the result. The concentration is only
an approximation. Press
EXIT
continue testing.
Note: If
EXIT
is not pressed the
meter will calibrate on the over
range result.
and
Overrange
<Enter> continue
Scan Blank
Scan Sample
Note: Over range results will also be
displayed in the test log.
12:00:00 001/500
STRAY LIGHT
The colorimeter should have no problems with stray light. Make sure that the
sample compartment lid is always fully closed.