LaMotte AUXILIARY PHOSPHORUS TEST KIT APT User Manual

AUX IL IARY PHOS PHO RUS TEST KIT

L

Mott

WARNING! This set contains chemicals

that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision

MODEL APT • CODE 7884

QUANTITY CONTENTS CODE

60 mL *Sulfuric Acid, 36% *7885-H
15 mL *Phenolphthalein Indicator, 1% *2246-E
120 mL *Sodium Hydroxide, 15% *7886-J
30 g *Ammonium Persulfate Reagent *6566-G
120 mL Deionized Water 5115PT-J
10 g Boiling Stones 7888-D
2 Pipets, 1 mL, plastic 0354
1 Spoon, 0.5 g, plastic 0698
1 Funnel 0459
2 Erlenmeyer Flasks, 125 mL, glass 0431
1 Filter paper, 100 sheets 0463
1 Graduated Cylinder, 50 mL, glass 0418

*WARNING: Reagents marked with a * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these
reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by
email, phone or fax.

To order individual reagents or test kit components, use the specified code
number.

All phosphorus determinations measure orthophosphate only. To
determine other forms of phosphorus in a sample, they must be separated
and converted to orthophosphate. The Auxiliary Phosphorus test
equipment is required to effect these separations and must be used in
conjunction with one of the standard phosphorus test kits such as Dual
Range Phosphate Kit (1-100 ppm PO
Phosphate Kit (0.05-1.0 ppm PO

-3

, Code 3320-01), or Low Range

4

-3

, Code 7416-01).

4

IN TRO DUC TION

The determination of orthophosphate (A), is made by testing a filtered
water sample. The determination of hydrolyzable phosphorus
(polyphosphate) and orthophosphate is made by testing a filtered water
sample which has been treated with a sulfuric acid digestion. This
converts hydrolyzable phosphorus (polyphosphate) to orthophosphates.
This determination (B) minus the orthophosphate determination (A) will
give the hydrolyzable or polyphosphate concentration.

A = Orthophosphate

B = Hydrolyzable Phosphorus + Orthophosphate

Hydrolyzable Phosphorus = B - A

The determination of total phosphorus (C) is made by testing a filtered
water sample which has been treated with a sulfuric acid/ammonium
persulfate digestion. This converts organic phosphorus and hydrolyzable
phosphorus to orthophosphates.

C = Total Phosphorus

Organic Phosphorus = C - B

The determination of particulate phosphorus and total phosphorus is
made by determining total phosphorus on an unfiltered water sample.

All of the comparator readings are given as PO

-3

. To convert this to

4

phosphorus, multiply by the factor 0.326.

PRO CE DURE

A. ORTHOPHOSPHATE

Filter the sample and test it for orthophosphate according to the
procedure given for the particular phosphate test kit being used. This is
reading A.

Orthophosphate = Reading A

B. HYDROLYZABLE PHOSPHORUS

(POLYPHOSPHATES) & ORTHOPHOSPHATES

1. Fill 50 mL graduated cylinder (0418) to 50 mL line with filtered
water. Pour into a 125 mL Erlenmeyer flask.

2. Use a 1 mL pipet (0354) to add 1 mL of *Sulfuric Acid, 36% (7885)
to flask. Swirl to mix.

3. Add a few boiling stones (7888). Place flask on hot plate and boil
gently for 30 minutes. Add Deionized Water (5115PT) to the sample
during the boiling step to maintain a volume between 10 and 50 mL.
Or microwave on high power for 2 minutes. Permit the volume to
decrease to approximately 10 mL (about c inch of water) by the end