Lamotte AUXILIARY PHOSPHORUS KIT User Manual

AUXILIARY PHOSPHORUS KIT

CODE 7884

QUANTITY CONTENTS CODE

60 mL *Sulfuric Acid, 36% *7885-H

15 mL *Phenolphthalein Indicator, 1% *2246-E

120 mL *Sodium Hydroxide, 15% *7886-J

30 g *Ammonium Persulfate Reagent *6566-G

120 mL Deionized Water 5115PT-J

10 g Boiling Stones 7888-D

2 Pipets, 1 mL, plastic 0354

1 Spoon, 0.5 g, plastic 0698

1 Funnel 0459

2 Erlenmeyer Flasks, 125 mL, glass 0431

1 Filter paper, 100 sheets 0463

1 Graduated Cylinder, 50 mL, glass 0418

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585)

To order individual reagents or test kit components, use the speciﬁ ed code number.

All phosphorus determinations measure orthophosphate only. To determine other forms of phosphorus in a sample, they must be separated and converted to orthophosphate. The Auxiliary Phosphorus test equipment is required to eﬀ ect these separations and must be used in conjunction with one of the standard phosphorus test kits such as Dual Range Phosphate Kit (1-100 ppm PO (0.05-1.0 ppm PO

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, Code 7416-02).

WARNING! This set contains chemicals

that may be harmful if misued. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision.

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, Code 4408-01), or Low Range Phosphate Kit

INTRODUCTION

The determination of orthophosphate (A), is made by testing a ﬁ ltered water sample. The determination of hydrolyzable phosphorus (polyphosphate) and orthophosphate is made by testing a ﬁ ltered water sample which has been treated with a sulfuric acid digestion. This converts hydrolyzable phosphorus (polyphosphate) to orthophosphates. This determination (B) minus the orthophosphate determination (A) will give the hydrolyzable or polyphosphate concentration.

A = Orthophosphate

B = Hydrolyzable Phosphorus + Orthophosphate

Hydrolyzable Phosphorus = B - A

The determination of total phosphorus (C) is made by testing a ﬁ ltered water sample which has been treated with a sulfuric acid/ammonium persulfate digestion. This converts organic phosphorus and hydrolyzable phosphorus to orthophosphates.

C = Total Phosphorus

Organic Phosphorus = C - B

The determination of particulate phosphorus and total phosphorus is made by determining total phosphorus on an unﬁ ltered water sample.

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All of the comparator readings are given as PO multiply by the factor 0.326.

. To convert this to phosphorus,

PROCEDURE

A. ORTHOPHOSPHATE

Filter the sample and test it for orthophosphate according to the procedure given for the particular phosphate test kit being used. This is reading A.

Orthophosphate = Reading A

B. HYDROLYZABLE PHOSPHORUS (POLYPHOSPHATES) & ORTHOPHOSPHATES

1. Fill a 50 mL graduated cylinder (0418) to 50 mL line with ﬁ ltered water. Pour into a 125 mL Erlenmeyer ﬂ ask.

2. Use a 1 mL pipet (0354) to add 1 mL of *Sulfuric Acid, 36% (7885) to ﬂ ask. Swirl to mix.

3. Add a few boiling stones (7888). Place ﬂ ask on hot plate and boil gently for 30 minutes. Add Deionized Water (5115PT) to the sample during the boiling step to maintain a volume between 10 and 50 mL. Or microwave on high power for 2 minutes. Permit the volume to decrease to approximately 10 mL (about ¼ inch of water) by the end of the boiling step. Do not allow the sample to go to dryness or to dense white sulfur trioxide fumes. Remove from heat and cool.

4. Add one drop of *Phenolphthalein Indicator, 1% (2246) to the cooled sample.

5. While swirling ﬂ ask, use a 1 mL pipet (0354) to add *Sodium Hydroxide, 15% (7886) dropwise until the solution turns faint pink. A volume of slightly less than 3 mL is required.

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