LaMotte AQ-5 User Manual

SALT WATER AQUACULTURE

TEST KIT

MODEL AQ-5 · CODE 3636-03

NOTE: It is important to read the instruction manual
before attempting to perform the tests with the short
form instructions provided below.

*WARNING: Reagents marked with a * are
considered to be potential health hazards. To view or
print a Material Safety Data Sheet (MSDS) for these
reagents see MSDS CD or www.lamotte.com. To obtain
a printed copy, contact LaMotte by e-mail, phone or
fax.

To order individual reagents or test kit components, use
the specified code number.

AM MO NIA NI TRO GEN

1. Fill test tube (0106) to 5 mL line with sample water.

2. Add 10 drops *Salicylate Ammonia #1 (3978LWT).

Cap and mix.

3. Add 7 drops of *Salicylate Ammonia #2 (3979WT).
Cap and mix. Wait 1 minute.

4. Add 7 drops of Salicylate Ammonia #3 (3982WT). Cap
and mix. Wait 20 minutes.

5. Insert test tube into Octa-Slide Viewer (1100) with
Ammonia Nitrogen Octa-Slide Bar (3438) inserted.

6. Record as ppm Ammonia Nitrogen (NH3-N).

NI TRITE NI TRO GEN

1. Fill test tube (0106) to 2.5 mL line with sample water.

2. Dilute to 5 mL line with *Mixed Acid Reagent

(V-6278).

3. Use 0.1g spoon (0699) to add 0.1g of *Color
Developing Reagent (V-6281). Cap and mix for 1
minute. Wait 5 minutes.

4. Insert test tube into Octa-Slide Viewer (1100) with
Nitrite Nitrogen Octa-Slide Bar (3437) inserted.

5. Record as ppm Nitrite Nitrogen (NO2-N).

NI TRATE NI TRO GEN

1. Fill test tube (0106) to 2.5 mL line with sample water.

2. Dilute to 5 mL line with *Mixed Acid Reagent

(V-6278). Cap and mix. Wait 2 minutes.

3. Use 0.1g spoon (0699) to add one level measure of
*Nitrate Reducing Reagent (V-6279). Cap and invert
tube 50-60 times in one minute. Wait 10 minutes.

4. Invert sample one time and insert test tube into
Octa-Slide Viewer (1100) with Nitrate Nitrogen
Octa-Slide Bar (3413) inserted.

5. Record as ppm Nitrate Nitrogen (NO3 -N).

AL KA LIN ITY

1. Fill titration tube (0608) to 5 mL line with sample
water.

2. Add 4 drops of *BCG-MR Indicator (2311-EG). Cap
and mix. Sample will turn blue-green.

3. Fill Direct Reading Titrator (0382) with *Alkalinity
Titration Reagent B (4493DR).

4. Titrate sample until blue-green color changes to pink.

5. Record as ppm Alkalinity (CaCO3).

CARBON DI OX IDE

1. Fill titration tube (0608) to 20 mL line with sample
water.

2. Add 2 drops *Phenolphthalein Indicator, 1% (2246). If
sample turns red, no free carbon dioxide is present. If
colorless, proceed to Step 3.

3. Fill Direct Reading Titrator (0380) with *Carbon
Dioxide Reagent B (4253DR).

4. Titrate sample until faint pink color persists for 30
seconds.

5. Record as ppm Carbon Dioxide (CO2).

LaMOTTE COM PANY

Helping Peo ple Solve An a lyt i cal Challenges

SM

800-344-3100 · 410-778-3100 (Out side U.S.A.) · Fax [phone] · www.lamotte.com

PO Box 329 · Chestertown · Mary land · 21620 · USA

3636-03-SF · 02/09