LaMotte AQ-4 User Manual
SALT WA TER
AQUACULTURE TEST KIT
MODEL AQ-4 · CODE 3635-03
NOTE:It is important to read the instruction
manual before attempting to perform the tests
with the short form instructions provided
below.
*WARNING: Reagents marked with an * are
considered potential health hazards. To view or
print a Material Safety Data Sheet (MSDS) for
these reagents see MSDS CD or
www.lamotte.com. To obtain a printed copy,
contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit
components, use the specified code number.
pH
1. Fill test tube (0106) to 5 mL line with sample
water.
2. Add 8 drops *Wide Range pH Indicator (2218).
Cap and mix.
3. Insert test tube into Octa-Slide Viewe r (1100).
Insert pH Octa-Slide Bar (3483). Match color.
4. Record as pH.
AMMONIA NITROGEN
1. Fill test tube (0106) to 5 mL line with sample
water.
2. Add 10 drops *Salicylate Ammonia #1
(3978L WT). Cap and mix.
3. Add 7 drops of *Salicylate Ammonia #2
(3979WT). Cap and mix. Wait 1 minute.
4. Add 7 drops of Salicylate Ammonia #3
(3982WT). Cap and mix wait 20 minutes.
5. Insert test tube into Octa-Slide Viewe r (1100).
Insert Ammonia Nitrogen Octa-Slide Bar (3441)
match color.
6. Record as ppm Ammonia Nitrogen (NH
-N).
3
NITRITE NITROGEN
1. Fill test tube (0106) to 2.5 mL line with sample
water.
2. Dilute to 5 mL line with *Mixed Acid Reagent
(V -6278).
3. Use 0.1g spoon (0699) to add 0.1g of *Color
Developing Reagent (V-6281). Cap and mix for
1 minute. Wait 5 minutes.
4. Place test tube into Octa-Slide Viewer (1100)
Insert Nitrite Nitrogen Octa-Slide Bar (3437).
Match color.
5. Record as ppm Nitrite Nitrogen (NO
2
-N).
NITRATE NITROGEN
1. Fill test tube (0106) to 2.5 mL line with sample
water.
2. Dilute to 5 mL line with *Mixed Acid Reagent
(V -6278). Cap and mix. Wait 2 minutes.
3. Use 0.1g spoon (0699) to add one level measure
of *Nitrate Reducing Reagent (V-6279). Cap
and invert 50-60 times in one minute. Wait 10
minutes.
4. Invert sample one time and insert test tube into
Octa-Slide Viewer (1100). Insert Nitrate
Nitrogen Octa-Slide Bar (3413). Match color.
5. Record as ppm Nitrate Nitrogen (NO
3
-N).
ALKALINITY
1. Fill titration tube (0608) to 5 mL line with
sample water.
2. Add 4 drops of *BCG-MR Indicator (2311-EG).
Cap and mix. Sample will turn blue-green.
3. Fill Direct Reading T itrator (0382) with
*Alkalinity T itration Reagent B (4493DR).
4. T itrate sample until blue-green color changes to
pink.
5. Record as ppm Alkalinity (CaCO
).
3
LaMOTTE COMPANY
PO Box 329 • Chestertown • Maryland • 21620 • USA
800-344-3100 • 410-778-3100 (Outside U.S.A.) • Fax 410-778-6394 • www.lamotte.com
CARBON DIOXIDE
1. Fill titration tube (0608) to 20 mL line with
sample water.
2. Add 2 drops *Phenolphthalein Indicator, 1%
(2246). If sample turns red, no free carbon
dioxide is present. If colorless, proceed to Step 3.
3. Fill Direct Reading T itrator (0380) with *Carbon
Dioxide Reagent B (4253DR).
4. T itrate sample until faint pink color persists for 30
seconds.
5. Record as ppm Carbon Dioxide (CO
).
2
SALINITY
1. Fill titration tube (0608) to 10 mL line with
demineralized water (1151).
2. Fill the 1.0 mL Titrator (0376) to 0 mark with
sample water. Wipe excess water off the T itrator .
3. Dispense 0.5 mL of sample water into titration
tube by depressing plunger until tip is at 0.5
mark. Discard remaining water in T itrator .
4. Add 3 drops of *Salinity Indicator Reagent A
(7460) to titration tube. Cap and gently swirl to
mix. Sample will turn yellow.
5. Fill T itrator (0378) with *Salinity T itration
Reagent B (7461).
6. T itrate sample until color changes from yellow to
pink-brown.
7. Record as ppt Salinity.
DISSOLVED OXYGEN
SAMPLING
1. Rinse sampling bottle (0688-DO). Replace cap.
2. Submerge bottle, then remove cap.
3. T a p sides of bottle to release air bubbles.
4. While bottle is submerged replace cap and
retrieve from water.
5. If air bubbles are present repeat sampling
method.
PRESERVATION
1. Add 8 drops of *Manganous Sulfate Solution
(4167).
2. Add 8 drops of *Alkaline Potassium Iodide
Azide Reagent (7166). Caution. Cap and mix by
inverting several times. Allow precipitate to
settle.
3. Add 8 drops of *Sulfuric Acid, 1:1 (6141WT).
4. Cap and mix until precipitate dissolves. Sample
is now “fixed”.
TEST PROCEDURE
1. Fill titration tube (0608) to 20 mL line with
“fixed” sample. Cap.
2. Fill Direct Reading T itrator (0377) with
*Sodium Thiosulfate, 0.025N (4169). Titrate
sample, swirling between each addition until
color is a very faint yellow.
3. Remove T itrator and cap. Use the pipet (0392)
to add 8 drops of Starch Indicator Solution
(4170PS). Sample should turn blue. Replace cap
and T itrator .
4. T itrate sample until blue color just disappears.
5. Record as ppm Dissolved Oxygen.
63635-03-SF • 4.08
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