LaMotte AQ-1 User Manual

AQ-1 • CODE 3559-01

Aquaculture

TEST KIT

LaMOTTE COMPANY

Helping People Solve Analytical Challenges

PO Box 329 • Chestertown • Maryland • 21620 • USA

800-344-3100 • 410-778-3100 (Outside USA) • Fax [phone]

Visit us on the web at www.lamotte.com

SM

03/06

QUANTITY CONTENTS CODE

30 mL Ammonia Nitrogen Reagent #1 4797-G
30 mL *Ammonia Nitrogen Reagent #2 *4798-G
120 mL *Mixed Acid Reagent *V-6278-J
10 g *Color Developing Reagent *V-6281-D
30 mL *Wide Range Indicator *2218-G
15 mL *Salinity Indicator Reagent A *7460-E
60 mL *Salinity Titration Reagent B *7461-H
30 mL *Manganous Sulfate Solution *4167-G
30 mL *Alkaline Potassium Iodide Azide *7166-G
50 g *Sulfamic Acid Powder *6286-H
60 mL Sodium Thiosulfate, 0.025N 4169-H
30 mL Starch Indicator Solution 4170WT-G
4 Square Tubes, plastic, w/cap 0106
1 Test Tube, glass, 5-10-15 mL, w/cap 0778
1 Test Tube, 5-10-12.9-15-20-25 mL,

0608

w/cap
1 Direct Reading Titrator, 0-10 Range 0377
1 Direct Reading Titrator, 0-20 Range 0378
1 Direct Reading Titrator, 0-1 Range 0376
1 Pipet, 0.5 mL, plastic 0353
1 Spoon, 0.1g, plastic 0699
1 Spoon, 1.0g, plastic 0697
1 Nitrite Nitrogen ColoRuler, 0.5-2.0 ppm 3551
1 Ammonia Nitrogen Color Chart,

3560-CC

0.5-2.0 ppm
1 pH Color Chart, 5.0-8.0 pH 3603-CC
1 Water Sampling Bottle, 60 mL, glass 0688-DO
1 Demineralizer Bottle 1151
1 Thermometer 1066

*WARNING: Reagents marked with a * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte
by email, phone or fax.

To order individual reagent refills or test kit components, use the specified code
numbers.

SA LIN ITY TEST

1. Fill test tube (0778) to 10 mL line with deionized water.

2. Fill the Direct Reading Titrator, 0-1 Range (0376) with sample water.

Wipe any excess sample water from the tip.

3. Dispense 0.5 mL of the sample water into the test tube by slowly
pressing the plunger until the tip of the plunger is opposite the 0.5
mark. Discard the remaining water in the Titrator.

4. Add 3 drops of *Salinity Indicator Reagent A (7460) to the test tube.
Cap and gently swirl to mix the contents.

5. Fill the Direct Reading Titrator, 0-20 Range (0378) with *Salinity
Titration Reagent B (7461). Insert into the center hole of the test tube
cap.

6. While gently swirling the test tube, slowly press the plunger to titrate
until the color of the solution changes from yellow to pink-brown.
Read the test result directly from the scale where the large ring on the
Titrator meets the Titrator barrel. Record as ppt salinity. Each minor
division equals 0.4.

EXAMPLE: Plunger tip is 3 minor divisions below line 10. The test
result is 10 plus (3 divisions x 0.4) equals 11.2 ppt.

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PROCEDURE

1. Fill the test tube (0608) to the 20 mL line with the “fixed” sample.
Cap.

NOTE: If the color of the “fixed” sample is already a very faint yellow,
skip Step 2, Perform Step 3, and begin the titration at Step 4.

2. Fill the Direct Reading Titrator, 0-10 Range (0377) with Sodium
Thiosulfate Solution (4169). Insert into the center hole of the test
tube cap.

3. While gently swirling the tube, slowly press the plunger to titrate until
the yellow-brown color is reduced to a very faint yellow.

4. Remove the Titrator and cap from the test tube. Be careful not to
disturb the Titrator plunger, as the titration begun in Step 3 will be
continued in Step 5. Add 8 drops of Starch Indicator Solution
(4170WT). Solution will turn blue.

5. Replace the cap and continue titrating until the blue color just
disappears. Read the test result directly from the scale where the large
ring on the Titrator meets the Titrator barrel. Record as ppm Dissolved
Oxygen. Each minor division equals 0.2 ppm.

6. If the plunger tip reached the bottom line on the titrator scale (10
ppm) before the endpoint color change occurs, refill the Titrator and
continue the titration. When recording the test result, be sure to
include the value of the original amount of reagent dispensed (10
ppm).

A Demineralizer Bottle supplies the deionized water used in this kit. Read
the Demineralizer Instruction Manual before proceeding.

Read the Direct Reading Titrator Instruction Manual before proceeding.

AM MO NIA NI TRO GEN TEST

1. Fill the square tube (0106) to the 5 mL line with sample water.

2. Add 8 drops of Ammonia Nitrogen Reagent #1 (4797).

3. Add 8 drops of *Ammonia Nitrogen Reagent #2 (4798). Cap and mix.

4. Allow 5 minutes for the color to develop. Invert once to mix and

remove the cap.

5. Hold the tube against the white area of the Ammonia Nitrogen Color
Chart (3560-CC). Match the sample color to a color standard. Record
as ppm Ammonia Nitrogen.

NOTE: If the resulting color is darker than the highest standard, a
dilution is required. Follow the dilution procedure below.

DILUTION PROCEDURE

1. Use the 0.5 mL pipet (0353) to add 0.5 mL of a new water sample to a
second square tube (0106).

2. Dilute to 5 mL line with deionized water.

3. Follow the instructions above, starting with Step 2. Multiply result by

10. Record as ppm Ammonia Nitrogen.

4. If the color is still darker than the highest standard use the 0.5 mL
pipet (0353) to add 0.5 mL of the sample to a square tube (0106) and
dilute to 10 mL.

5. Use 5 mL of this diluted sample and follow instructions above, starting
with Step 2.

6. Multiply result by 20. Record as ppm Ammonia Nitrogen.

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NITRITE NI TRO GEN TEST

NOTE: Place dispenser cap (0692) on *Mixed Acid Reagent (V-6278).
Save this cap for refill reagents.

1. Fill a square tube (0106) to the 2.5 mL line with sample water.

2. Dilute to 5 mL line with *Mixed Acid Reagent (V-6278).

3. Use the 0.1g spoon (0699) to add 0.1g of *Color Developing Reagent

(V-6281). Cap and mix for one minute.

4. Face natural light. Holding the Nitrite
Nitrogen ColoRuler (3351) in an upright
position, place the bottom of the tube on
the ridge above the standards. Match
sample color to a color standard. Record as
ppm Nitrite Nitrogen.

NOTE: If the resulting color is darker than
the highest standard, a dilution is required.
Follow the dilution procedure below.

DILUTION PROCEDURE

1. Use the 0.5 mL pipet (0353) to add 0.5 mL of sample water to a
second square tube (0106).

2. Dilute to 5 mL line with deionized water.

3. Use 2.5 mL of the diluted sample and follow the instructions above,

starting with Step 2. Multiply result by 10. Record as ppm nitrate
nitrogen.

4. If the color is still darker than the highest standard use the 0.5 mL
pipet (0353) to add 0.5 mL of the sample to a tube and dilute to 10
mL.

5. Use 2.5 mL of this diluted sample and follow instructions starting with
Step 2.

6. Multiply result by 20. Record as ppm Nitrite Nitrogen.

pH

1. Fill a square tube (0106) to the 5 mL line with sample water.

2. Add 8 drops of *Wide Range Indicator (2218). Cap and mix.

3. Hold the tube against the white area of the pH Color Chart

(3603-CC). Match sample color to a color standard. Record the results
as pH.

DIS SOLVED OX Y GEN TEST

COLLECTING THE WATER SAMPLE

1. To avoid contamination, rinse the Water Sampling Bottle (0688-DO)
thoroughly with sample water.

2. Tightly cap the bottle. Submerge to the desired depth, and remove the
cap to allow the bottle to fill.

3. Tap the sides of the submerged bottle to dislodge any air bubbles
clinging to the inside of the bottle. Replace the cap while the bottle is
still submerged.

4. Retrieve the bottle. Examine it carefully to make sure that no air
bubbles are trapped inside. Once a satisfactory sample has been
collected, proceed immediately with Steps 5 and 6 to “fix” the sample.

“FIXING” THE WATER SAMPLE

NOTE: Be careful not to introduce air into the sample
while adding the reagents in Steps 5 and 6. Simply drop
the reagents into the test sample, cap carefully, and mix
gently.

5. Add 8 drops of *Manganous Sulfate Solution
(4167) and 8 drops of *Alkaline Potassium Iodide
Azide Solution (7166). Cap and mix by inverting
gently several times.

A precipitate will form. Allow the precipitate to
settle below the shoulder of the bottle before
proceeding.

6. Use the 1.0g spoon (0697) to add one level
measure of *Sulfamic Acid Powder (6286). Cap and gently shake to
mix, until both the reagent and the precipitate have dissolved. A clear
yellow to brown-orange color will develop, depending on the oxygen
content of the sample.

NOTE: Following the completion of Step 6, contact between the
water sample and the atmosphere will not affect the test result. Once
the sample has been “fixed” in this manner, it is not necessary to
perform the actual test procedure immediately.

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