LaMotte AMMONIA NITROGEN TEST KIT SAN User Manual

AM MO NIA NI TRO GEN TEST KIT

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SALICYLATE METHOD

MODEL SAN · CODE 7451

QUANTITY CONTENTS CODE

60 mL *Citrate Buffer *7453-H
10 g *Salicylate Reagent *7454-D
5 g *Isocyanurate Reagent Powder *7455-C
1 Pipet, 1 mL, plastic 0354
1 Pipet, 0.5 mL, plastic 0353
1 Spoon, 0.15 g, plastic 0727
1 Spoon, 0.10 g, plastic 0699
2 Test Tubes, 5 mL, glass 0230
1 Ammonia Nitrogen Comparator, 0.0-1.0 ppm 7452

*WARNING: Reagents marked with an * are considered hazardous substances.
To view or print a Material Safety Data Sheet (MSDS) for these reagents see
MSDS CD or our web site. To obtain a printed copy, contact us by e-mail,
phone, or fax.

To order individual reagents or test kit components, use the specified code
number.

USE OF THE OCTET COM PARA TOR

The Octet Comparator contains eight permanent
color standards. A test sample is inserted into the
openings in the top of the comparator. The
sample can then be compared to four color
standards at once, and the value read off the
comparator. For optimum color comparison, the
comparator should be positioned between the
operator and a light source, so that the light
enters through the special light-diffusing screen
in the back of the comparator. Avoid viewing the
comparator against direct sunlight or an
irregularly lighted background.

PRO CE DURE

1. Fill test tube (0230) to 5 mL line with sample water.

2. Use the plastic pipet (0354) to add 1 mL of *Citrate Buffer (7453).

3. Use the 0.15 g spoon (0727) to add one level measure of *Salicylate

Reagent (7454). Cap and mix. Wait one minute. Solution will turn
yellow.

4. Use the 0.1 g spoon (0699) to add one level measure of *Isocyanurate
Reagent Powder (7455). Cap and shake vigorously until all solid has
dissolved. Wait at least 12 minutes until green color develops.

5. Place test tube into the Ammonia Nitrogen Comparator (7452).
Match sample color with a color standard. Record as ppm
Ammonia-Nitrogen. If the sample color is between two standards,
the midpoint between the standards is taken as the result.

6. If sample is darker than the 1.0 ppm color standard, the sample must
be diluted and retested. Use the 0.5 mL pipet (0353) to add 0.5 mL of
sample water to test tube. Dilute to 5 mL line with distilled or
deionized water. Follow Steps 2-5. Multiply reading by 10. Record as
ppm Ammonia Nitrogen.

7. To express results as Unionized Ammonia (NH3):

Unionized Ammonia (NH3) =

ppm Ammonia Nitrogen (NH3-N) x 1.2

To express results as Ionized Ammonia (NH

Ionized Ammonia (NH

ppm Ammonia Nitrogen (NH3-N) x 1.3

Ammonia in water occurs in two forms: toxic unionized ammonia (NH3)
and the relatively non-toxic form, ammonium ion (NH

+

):

4

+

) =

4

+

). This test

4

method measures both forms as ammonia-nitrogen (NH3-N) to give the
total ammonia-nitrogen concentration in water. The actual proportion of
each compound depends on temperature, salinity, and pH. A greater
concentration of unionized ammonia is present when the pH value and
salinity increase.

1. Consult the table below to find the percentage that corresponds to
the temperature, pH and salinity of the sample.

2. To express the test result as ppm Unionized Ammonia Nitrogen
(NH3-N), multiply the total ammonia-nitrogen test result by the
percentage from the table.

3. To express the test result as ppm Ionized Ammonia Nitrogen

+

(NH

-N), subtract the unionized ammonia nitrogen from the total

4

ammonia-nitrogen.