LaMotte AMMONIA NITROGEN TEST KIT NANR User Manual

AM MO NIA NI TRO GEN TEST KIT

WARNING! This set contains chemicals

that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision

LOW RANGE METHOD

MODEL NANR • CODE 7418-02

QUANTITY CONTENTS CODE

30 mL Ammonia Nitrogen Reagent #1 4797WT-G

2 x 30 mL *Ammonia Nitrogen Reagent #2 *4798PS-G

1 Pipet, 1.0 mL, plastic 0354

3 Test Tubes, 10 mL, glass, w/caps 0843

1 Distilled Water Ampoule, 5 mL 2748

1 Axial Reader 2071

1 Ammonia Nitrogen Comparator

0.02-1.0 ppm

*WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by email, phone or fax.

To order individual reagents or test kit components, use the specified code number.

NOTE: To compensate for color produced by the reagents, a blank should be run on deionized or distilled water. Any reading obtained should be subtracted from the test result.

NOTE: Check treatment chemical label for contents and warning. Certain aquarium treatment chemicals interfere with this Nesslerization Method. Some ketones, aldehydes, alcohols, and amines cause interferences.

Read Axial Reader Instruction Manual before proceeding.

7593

USE OF THE OCTET COM PARA TOR

Mott

The Octet Comparator contains eight permanent color standards. A test sample is inserted into the openings in the top of the comparator. The sample can then be compared to four color standards at once, and the value read off the comparator. For optimum color comparison, the comparator should be positioned between the operator and a light source, so that the light enters through the special light-diffusing screen in the back of the comparator. Avoid viewing the comparator against direct sunlight or an irregularly lighted background.

PART 1 - PRO CE DURE

1. Rinse a test tube (0843) with sample water. Fill to 10 mL line with sample water.

2. Add 8 drops of Ammonia Nitrogen Reagent #1 (4797). Cap and mix. Wait 1 minute.

NOTE: When testing salt (sea) water, increase the amount of Ammonia Nitrogen Reagent #1 to 20 drops.

3. Use the pipet (0354) to add 1 mL of *Ammonia Nitrogen Reagent #2 (4798). Cap and mix. Wait five minutes. Remove cap.

NOTE: When testing salt water, the reading must be made in one minute to avoid interference from a precipitate which may form.

4. Insert the sample tube into the Axial Reader (2070). Match sample color to a color standard. Record as ppm Total Ammonia Nitrogen.

To express results as Unionized Ammonia (NH3):

ppm Ammonia Nitrogen (NH3–N) x 1.2 = Ammonia (NH4)

To express resluts as Ionized Ammonium (NH

ppm Ammonia Nitrogen (NH3–N) x 1.3 = Ammonium (NH

)

5. If the color of the test sample is darker than the highest color

standard, repeat the test on a sample diluted 1 to 1 with ammonia-free or distilled water. Multiply the reading by a factor of 2.

PART 2 - CON VER SIONS

Ammonia in water occurs in two forms: toxic unionized ammonia (NH3), and the relatively non-toxic ionized form, ammonium ion (NH method measures both forms as ammonia-nitrogen (NH total ammonia-nitrogen concentration in water. The actual proportion of each compound depends on temperature, salinity, and pH. A greater

). This test

–N) to give the

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