AM MO NIA NI TRO GEN TEST KIT
L
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o
tt
WARNING! This set contains chemicals
that may be harmful if misused. Read
cautions on individual containers
carefully. Not to be used by children
except under adult supervision
LOW RANGE METHOD
MODEL NANR • CODE 7418-02
QUANTITY CONTENTS CODE
30 mL Ammonia Nitrogen Reagent #1 4797WT-G
2 x 30 mL *Ammonia Nitrogen Reagent #2 *4798PS-G
1 Pipet, 1.0 mL, plastic 0354
3 Test Tubes, 10 mL, glass, w/caps 0843
1 Distilled Water Ampoule, 5 mL 2748
1 Axial Reader 2071
1 Ammonia Nitrogen Comparator
0.02-1.0 ppm
*WARNING: Reagents marked with a * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see
MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by email,
phone or fax.
To order individual reagents or test kit components, use the specified code number.
NOTE: To compensate for color produced by the reagents, a blank should be
run on deionized or distilled water. Any reading obtained should be subtracted
from the test result.
NOTE: Check treatment chemical label for contents and warning. Certain
aquarium treatment chemicals interfere with this Nesslerization Method.
Some ketones, aldehydes, alcohols, and amines cause interferences.
Read Axial Reader Instruction Manual before proceeding.
7593
USE OF THE OCTET COM PARA TOR
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Mott
The Octet Comparator contains eight permanent
color standards. A test sample is inserted into the
openings in the top of the comparator. The sample
can then be compared to four color standards at once,
and the value read off the comparator. For optimum
color comparison, the comparator should be
positioned between the operator and a light source, so
that the light enters through the special light-diffusing
screen in the back of the comparator. Avoid viewing
the comparator against direct sunlight or an irregularly
lighted background.
PART 1 - PRO CE DURE
1. Rinse a test tube (0843) with sample water. Fill to 10 mL line with
sample water.
2. Add 8 drops of Ammonia Nitrogen Reagent #1 (4797). Cap and mix.
Wait 1 minute.
NOTE: When testing salt (sea) water, increase the amount of
Ammonia Nitrogen Reagent #1 to 20 drops.
3. Use the pipet (0354) to add 1 mL of *Ammonia Nitrogen Reagent #2
(4798). Cap and mix. Wait five minutes. Remove cap.
NOTE: When testing salt water, the reading must be made in one
minute to avoid interference from a precipitate which may form.
4. Insert the sample tube into the Axial Reader (2070). Match sample
color to a color standard. Record as ppm Total Ammonia Nitrogen.
To express results as Unionized Ammonia (NH3):
ppm Ammonia Nitrogen (NH3–N) x 1.2 = Ammonia (NH4)
To express resluts as Ionized Ammonium (NH
ppm Ammonia Nitrogen (NH3–N) x 1.3 = Ammonium (NH
+
):
4
+
)
4
5. If the color of the test sample is darker than the highest color
standard, repeat the test on a sample diluted 1 to 1 with
ammonia-free or distilled water. Multiply the reading by a factor of 2.
PART 2 - CON VER SIONS
Ammonia in water occurs in two forms: toxic unionized ammonia (NH3), and
the relatively non-toxic ionized form, ammonium ion (NH
method measures both forms as ammonia-nitrogen (NH
total ammonia-nitrogen concentration in water. The actual proportion of
each compound depends on temperature, salinity, and pH. A greater
+
). This test
4
+
–N) to give the
4
concentration of unionized ammonia is present when the pH value and
salinity increase.
1. Consult the table to find the percentage that corresponds to the
temperature, pH and salinity of the sample.
2. To express the test result as ppm Unionized Ammonia Nitrogen
(NH3–N), multiply the total ammonia-nitrogen test result by the
percentage from the table.
3. To express the test result as ppm Ammonia Nitrogen (NH
+
4
–N),
subtract the unionized ammonia-nitrogen determined in Step 2 from
the total ammonia-nitrogen.
Percentage of Free Ammonia as (NH3) in Freshwater1 (FW)
and Seawater2 (SW) at varying pH and Temperature
10°C 15°C 20°C 25°C
pH FW SW FW SW FW SW FW SW
7.0 0.19 0.27 0.40 0.55
7.1 0.23 0.34 0.50 0.70
7.2 0.29 0.43 0.63 0.88
7.3 0.37 0.54 0.79 1.10
7.4 0.47 0.68 0.99 1.38
7.5 0.59 0.459 0.85 0.665 1.24 0.963 1.73 1.39
7.6 0.74 0.577 1.07 0.836 1.56 1.21 2.17 1.75
7.7 0.92 0.726 1.35 1.05 1.96 1.52 2.72 2.19
7.8 1.16 0.912 1.69 1.32 2.45 1.90 3.39 2.74
7.9 1.46 1.15 2.12 1.66 3.06 2.39 4.24 3.43
8.0 1.83 1.44 2.65 2.07 3.83 2.98 5.28 4.28
8.1 2.29 1.80 3.32 2.60 4.77 3.73 6.55 5.32
8.2 2.86 2.26 4.14 3.25 5.94 4.65 8.11 6.61
8.3 3.58 2.83 5.16 4.06 7.36 5.78 10.00 8.18
8.4 4.46 3.54 6.41 5.05 9.09 7.17 12.27 10.10
8.5 5.55 4.41 7.98 6.28 11.18 8.87 14.97 12.40
1
Freshwater data from Trussel (1972).
2
Seawater values from Bower and Bidwell (1978). Salinity of Seawater values = 34% at an ionic
strength of 0.701 m.
FOR EXAMPLE:
A fresh water sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as
total Ammonia-Nitrogen.
1. The percentage from the table is 11.18% (or 0.1118).
2. 1 ppm total Ammonia-Nitrogen x 0.1118 = 0.1118 ppm Unionized
Ammonia-Nitrogen
3.
Total Ammonia-Nitrogen 1.0000 ppm
Unionized Ammonia-Nitrogen
0.1118 ppm
-
Ionized Ammonia-Nitrogen = 0.8882 ppm
LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Chal lenges
PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
SM
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