Lamotte AMMONIA-NITROGEN KIT User Manual

AMMONIA-NITROGEN KIT

SALICYLATE METHOD, OCTA-SLIDE 2, 0-2 ppm

CODE 3304-02

QUANTITY CONTENTS CODE

60 mL *Salicylate Ammonia #1 *3978LWT-H

30 mL *Salicylate Ammonia #2 *3979WT-G

30 mL Salicylate Ammonia #3 3982WT-G

2 Test Tubes, plastic, w/caps 0106

1 Ammonia-Nitrogen Octa-Slide 2 Bar, 0-2 ppm,

Fresh Water

1 Ammonia-Nitrogen Octa-Slide 2 Bar, 0-2 ppm,

Salt Water

1 Octa-Slide 2 Viewer 1101

*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the contents
list or in the test procedures. Omit any letter that follows or precedes the four digit code
number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy,
contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents
is available from Chem-Tel:
(US, 1-800-255-3924)
(International, call collect, 813-248-0585)

To order individual reagents or test kit components,

Warning! This set contains chemicals
that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision.

use the specied code number.

3441-01-FW

3441-01-SW

USE OF THE OCTA-SLIDE 2 VIEWER

The Octa-Slide 2 Viewer should be held so non-direct
light enters through the back of the Viewer.
Octa-Slide 2
sample into
the

reaction to the color standards.

Slide the

Bar into the Viewer. Insert the reacted

the top of the Viewer. Match the color of

PROCEDURE

1

4

Cap and mix.

7

Insert
Ammonia
Nitrogen Octa­Slide 2 Bar
(3441-01-FW
or 3441-01­SW) into the
Octa-Slide 2
Viewer (1101).

Wait 1 minute.

01:00

2

Fill a test tube
(0106) to the
5 mL line with
sample water.

5

Add 7 drops
of *Salicylate
Ammonia #2
(3979WT).

8

Add 7 drops
of Salicylate
Ammonia #3
(3982WT).

3

Add 10 drops
of *Salicylate
Ammonia #1
(3978LWT).

6

Cap and mix.

9

Cap and mix.

10

Wait 20 minutes.

20:00

11

Insert test tube
into Octa-Slide 2
Viewer (1101).

12

Match sample
color to a
color standard.
Record as ppm
Ammonia
Nitrogen
(NH3-N).

CONVERSIONS

Ammonia in water occurs in two forms: toxic unionized ammonia (NH3), and the
relatively non-toxic ionized form, ammonium ion (NH
both forms as ammonia-nitrogen (NH

+

–N) to give the total ammonia-nitrogen

3

concentration in water. The actual proportion of each compound depends on temperature,
salinity, and pH. A greater concentration of unionized ammonia is present when the
pH value and salinity increase.

1. Consult the table (next page) to nd the percentage that corresponds to the
temperature, pH and salinity of the sample.

2. To express the test result as ppm Unionized Ammonia Nitrogen (NH
the total ammonia-nitrogen test result by the percentage from the table.

3. To express the test result as ppm Ammonia Nitrogen (NH
unionized ammonia-nitrogen determined in Step 2 from the total ammonia-nitrogen.

Percentage of Free Ammonia as (NH3) in Freshwater1 (FW)

and Seawater2 (SW) at varying pH and Temperature

10°C 15°C 20°C 25°C

pH FW SW FW SW FW SW FW SW

7.0 0.19 0.27 0.40 0.55

7.1 0.23 0.34 0.50 0.70

7.2 0.29 0.43 0.63 0.88

7.3 0.37 0.54 0.79 1.10

7.4 0.47 0.68 0.99 1.38

7.5 0.59 0.459 0.85 0.665 1.24 0.963 1.73 1.39

7.6 0.74 0.577 1.07 0.836 1.56 1.21 2.17 1.75

7.7 0.92 0.726 1.35 1.05 1.96 1.52 2.72 2.19

7.8 1.16 0.912 1.69 1.32 2.45 1.90 3.39 2.74

7.9 1.46 1.15 2.12 1.66 3.06 2.39 4.24 3.43

8.0 1.83 1.44 2.65 2.07 3.83 2.98 5.28 4.28

8.1 2.29 1.80 3.32 2.60 4.77 3.73 6.55 5.32

8.2 2.86 2.26 4.14 3.25 5.94 4.65 8.11 6.61

8.3 3.58 2.83 5.16 4.06 7.36 5.78 10.00 8.18

8.4 4.46 3.54 6.41 5.05 9.09 7.17 12.27 10.10

8.5 5.55 4.41 7.98 6.28 11.18 8.87 14.97 12.40

1

Freshwater data from Trussel (1972).

2

Seawater values from Bower and Bidwell (1978).

Salinity of Seawater values = 34% at an ionic strength of 0.701 m.

+

). This test method measures

4

–N), multiply

3

+

–N), subtract the

3

FOR EXAMPLE:

A fresh water sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as total
Ammonia-Nitrogen.

1. The percentage from the table is 11.18% (or 0.1118).

2. 1 ppm total Ammonia-Nitrogen x 0.1118 = 0.1118 ppm Unionized Ammonia­Nitrogen

3. Total Ammonia-Nitrogen 1.0000 ppm

Unionized Ammonia-Nitrogen - 0.1118 ppm

Ionized Ammonia-Nitrogen = 0.8882 ppm

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63304-02 4/19