LaMotte ALGAE IN WATER TEST KIT AWL User Manual

ALGAE IN WA TER TEST KIT

WARNING! This set contains chemicals

that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision

MODEL AWL • CODE 6662

QUANTITY CONTENTS CODE

2 x 60 mL *Methyl Alcohol *6319-H
1 Test Tube, 5-10-12.9-20-25 mL, glass, w/cap 0608
2 Test Tubes, 5 mL, glass 0230
1 Syringe, 60 mL 0943
1 Filter Holder, plastic 0598
1 Check Valve 27760-01
1 Filter paper, glass 1157
1 Tygon Tubing, w/adapter 1175

*WARNING: Reagents marked with a * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see
MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by email,
phone or fax.

To order individual reagents or test kit components, use the specified code number.

IN TRO DUC TION

This test kit uses a unique filtration procedure for determining if there is an
unacceptable level of algae in water. The algae cells are filtered out of the water by
means of a syringe with filter attachment. A yellow-green color on the glass fiber
filter indicates algae may be present, since minerals which may also cause
coloration of the water will not be trapped on the filter. The algae collected on
the filter is then subjected to an alcolhol extraction procedure. A
yellow to green color in the extract solution indicates an
unacceptable level of algae in the water.

FIL TRA TION AS SEM BLY

1. Unscrew the cover of the filter holder (0598) and install a

filter paper (1157) in the holder. Position the paper
carefully to avoid by-passing the filter. If a membrane filter
is used, install a support pad in back of the membrane disc.
Replace the cover.

2. Insert the end of the check valve (27760-01), that does not have threads and

does have small tabs, into the Luer tip of the syringe (0943).

3. Insert the opposite end of the check valve with the threads into the

larger opening in the filter holder.

4. Depress the syringe plunger to the 0 position. Attach the plastic tubing

(1175) to the side arm of the check valve. Submerge the free end of the
tubing in the sample water.

PRO CE DURE

1. After the syringe-filter device has been prepared according to above

procedure, complete one stroke of the syringe by pulling the plunger out
and drawing water into the syringe through the plastic tubing. Fill the
barrel to the 50 mL mark. Slowly depress the plunger to expel the water
through the filter holder. Repeat at least 4 times.
NOTE: When excessive pressure is exerted upon the filter disc by
depressing the plunger too rapidly, there is a tendency for the filter disc to
rupture. If this happens, replace filter disc and repeat sampling procedure.

2. Disconnect filter holder from the syringe. Unscrew the filter holder and

carefully remove the filter disc. The presence of a green-yellow color on the
filter disc indicates algae may be present.

3. Place the filter disc in the test tube (0608). Fill to 5 mL line with

*Methyl Alcohol (6319). Cap and shake vigorously for approximately 2
minutes. This will extract the green chlorophyll from the algae cells and
disintegrate the disc. The disintegrated disc must be filtered out of the
solution.

4. Place a new filter disc in the filter holder.

5. Remove the check valve from syringe. Attach the filter holder directly to

syringe.

6. Remove the plunger from the syringe and pour the contents of the test tube

into the syringe barrel. Place the outlet of the filter holder into the test tube
(0230), replace the plunger in the syringe, and slowly depress until all of the
solution has been collected in the test tube.

7. View across the diameter of the test tube or down through the solution.

A clear yellow to green color indicates algae is present.

LaMOTTE COM PANY

Helping Peo ple Solve An a lyt i cal Challenges

PO Box 329 • Chestertown • Mary land • 21620 • USA

800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax [phone]

Visit us on the web at www.lamotte.com

SM

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