LaMotte ALGAE IN WATER KIT User Manual
ALGAE IN WATER KIT
FILTRATION/EXTRACTION METHOD
CODE 6662-01
QUANTITY CONTENTS CODE
2 x 60 mL *Methyl Alcohol *6319-H
1 Test Tube, 5-10-12.9-20-25 mL, glass, w/cap 0608
2 Test Tubes, 5 mL, glass 0230
1 Syringe, 60 mL 0943
1 Filter Holder, plastic 0598
1 Check Valve 27760-01
1 Filter paper, glass 1157
1 Tygon Tubing, w/adapter 1175
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents go to
www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specifi ed code number.
WARNING! This set contains chemicals
that may be harmful if misued. Read
cautions on individual containers
carefully. Not to be used by children
except under adult supervision.
INTRODUCTION
This test kit uses a unique fi ltration procedure for determining if there is an
unacceptable level of algae in water. The algae cells are fi ltered out of the water by
means of a syringe with fi lter attachment. A yellow-green color on the glass fi ber
fi lter indicates algae may be present, since minerals which may also cause coloration
of the water will not be trapped on the fi lter. The algae collected on the fi lter is then
subjected to an alcolhol extraction procedure. A yellow to green color in the extract
solution indicates an unacceptable level of algae in the water.
FILTRATION ASSEMBLY
1. Unscrew the cover of the fi lter holder (0598) and install a fi lter paper (1157) in the
holder. Position the paper carefully to avoid by-passing the fi lter. If a membrane
fi lter is used, install a support pad in back of the membrane disc. Replace the cover.
2. Insert the end of the check valve (27760-01), that does not have threads and does
have small tabs, into the Luer tip of the syringe (0943).
3. Insert the opposite end of the check valve with the threads into the larger opening
in the fi lter holder.
4. Depress the syringe plunger to the 0 position. Attach the plastic tubing (1175) to
the side arm of the check valve. Submerge the free end of the tubing in the sample
water.
PROCEDURE
1. After the syringe-fi lter device has been prepared according to above procedure,
complete one stroke of the syringe by pulling the plunger out and drawing water
into the syringe through the plastic tubing. Fill the barrel to the 50 mL mark.
Slowly depress the plunger to expel the water through the fi lter holder. Repeat at
least 4 times.
NOTE: When excessive pressure is exerted upon the fi lter disc by depressing
the plunger too rapidly, there is a tendency for the fi lter disc to rupture. If this
happens, replace fi lter disc and repeat sampling procedure.
2. Disconnect fi lter holder from the syringe. Unscrew the fi lter holder and carefully
remove the fi lter disc. The presence of a green-yellow color on the fi lter disc
indicates algae may be present.
3. Place the fi lter disc in the test tube (0608). Fill to 5 mL line with *Methyl
Alcohol (6319). Cap and shake vigorously for approximately 2 minutes. This will
extract the green chlorophyll from the algae cells and disintegrate the disc. The
disintegrated disc must be fi ltered out of the solution.
4. Place a new fi lter disc in the fi lter holder.
5. Remove the check valve from syringe. Attach the fi lter holder directly to syringe.
6. Remove the plunger from the syringe and pour the contents of the test tube into
the syringe barrel. Place the outlet of the fi lter holder into the test tube (0230),
replace the plunger in the syringe, and slowly depress until all of the solution has
been collected in the test tube.
7. View across the diameter of the test tube or down through the solution. A clear
yellow to green color indicates algae is present.
LaMOTTE COMPANY
Helping People Solve Analytical Challenges
PO Box 329 • Chestertown • Maryland • 21620 • USA
800-344-3100 • 410-778-3100 (Outside U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
66662-01 5/12
®
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