LaMotte AG-104 User Manual

This booklet provides step-by-step instructions for each of the tests

provided in the AG-104 test kit. Please refer to the separate sheets for proper use of the Direct Reading Titrator and Axial Reader Comparator where noted.

Use of the pH Oc tet Com para tor

The Octet Comparator contains eight permanent color standards. A test sample is inserted into the openings in the top of the comparator. The sample can then be compared to four color standards at once, and the value read off the comparator. For optimum color comparison, the comparator should be positioned between the operator and a light source, so that the light enters through the special light-diffusing screen in the back of the comparator. Avoid viewing the comparator against direct sunlight or an irregularly lighted background.

Use of the Octa-Slide Viewer

The Octa-Slide Viewer should be held so non-direct light enters through the back of the comparator. With sample tube inserted at top, slide the Octa-Slide bar thorough the viewer and march with color standard.

TA BLE OF CON TENTS

Alkalinity................................................................................................ 3

Ammonia Nitrogen........................................................................... 4 - 5

Carbon Dioxide ...................................................................................... 6

Dissolved Oxygen............................................................................ 7 - 11

Nitrate Nitrogen................................................................................... 11

Nitrite ................................................................................................... 12

pH ......................................................................................................... 13

Salinity .......................................................................................... 14 - 15

ALKALINITY

QUAN TITY CON TENTS CODE

100 BCG-MR Indicator Tablets T-2311-J 60 mL *Alkalinity Titration Reagent B *4493DR-H 1 Test Tube, 5-10-15 mL, glass w/cap 0778 1 Direct Reading Titrator, 0-200 Range 0382

*WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by email, phone or fax.

NOTE: Read Direct Reading Titrator Manual before performing test.

Pro ce dure

1. Fill the test tube (0778) to 5 mL line with sample water.

2. Add one BCG-MR Indicator Tablet (T-2311). Cap and shake until dissolved. A blue-green color will develop.

3. Fill the Direct Reading Titrator (0382) with *Alkalinity Titration Reagent B (4493DR). Insert Titrator into the center hole of the test tube cap.

4. While gently swirling the tube, slowly depress plunger to titrate until the blue-green color changes to pink. Read the test result directly from the scale where the large ring on the Titrator meets the Titrator barrel. Record results as Total Alkalinity in ppm (CaCO3). Each minor division on Titrator scale = 4 ppm Total Alkalinity as CaCO3.

EXAMPLE: Plunger tip is 3 minor divisions below line 140. The test result is 140 plus (3 divisions x 4) equals 152 ppm.

5. If the plunger tip reaches the bottom line on the titrator scale (200 ppm) before the endpoint color change occurs, refill the titrator and continue titration. When recording the test result, be sure to include the value of the original amount of reagent dispensed (200 ppm).

When testing salt water aquariums, alkalinity may be called carbonate hardness. Multiply ppm Alkalinity by 0.056 to convert to German degrees of Hardness.

This test set provides Total Alkalinity readings only. For comprehensive analysis of Phenolphthalein (P), Total (T), Hydroxide, Carbonate, and Bicarbonate Alkalinity the Model WAT-MP-DR Test Set, Code 4533-DR is recommended.

AMMONIA NITROGEN

Salicylate Method

QUAN TITY CON TENTS CODE

60 mL *Salicylate Ammonia #1 *3978LWT-H 30 mL *Salicylate Ammonia #2 *3979WT-G 30 mL Salicylate Ammonia #3 3982WT-G 2 Test Tubes, plastic, w/cap 0106 1 Octa-Slide Viewer 1100 1 Ammonia-Nitrogen Octa-Slide Bar,

0-2 ppm

Pro ce dure

1. Fill a test tube (0106) to the 5 mL line with the water sample.

2. Add 10 drops of Salicylate Ammonia #1 (3978LWT). Cap and mix.

3. Add 7 drops of *Salicylate Ammonia #2 (3979WT). Cap and mix. Wait one minute.

4. Add 7 drops of Salicylate Ammonia #3 (3982). Cap and mix. Wait 20 minutes.

5. Insert Ammonia Nitrogen Octa-Slide Bar (3441) into the Octa-Slide Viewer (1100). Insert test tube into Octa-Slide Viewer. Match sample color to a color standard. Record as ppm Ammonia Nitrogen (NH

N).

To express results as Unionized Ammonia (NH3):

ppm Unionized Ammonia (NH3) =

ppm Ammonia Nitrogen (NH3–N) x 1.2

To express results as Ionized Ammonia (NH4):

ppm Ionized Ammonia (NH

) =

ppm Ammonia Nitrogen (NH3–N) x 1.3

Ammonia in water occurs in two forms: toxic unionized ammonia (NH3) and the relatively non-toxic ionized form, ammonium ion

(NH

). This test method measures both forms as ammonia nitrogen

(NH

–N) to give the total ammonia nitrogen concentration in water.

The actual proportion of each compound depends on temperature, salinity, and pH. A greater concentration of unionized ammonia is present when the pH value and salinity increase.

3441

Consult the table to find the percentage that corresponds to the temperature, pH, and salinity of the sample.

2. To express the test result as ppm Unionized Ammonia Nitrogen (NH3–N), multiply the total ammonia-nitrogen test result by the percentage from the table.

3. To express the test result as ppm Ionized Ammonia Nitrogen

(NH

–N), subtract the unionized ammonia-nitrogen determined in

Step 2 from the total ammonia-nitrogen.

10°C 15°C 20°C 25°C

pH FW1SW

FW SW FW SW FW SW

7.0 0.19 0.27 0.40 0.55

7.1 0.23 0.34 0.50 0.70

7.2 0.29 0.43 0.63 0.88

7.3 0.37 0.54 0.79 1.10

7.4 0.47 0.68 0.99 1.38

7.5 0.59 0.459 0.85 0.665 1.24 0.963 1.73 1.39

7.6 0.74 0.577 1.07 0.836 1.56 1.21 2.17 1.75

7.7 0.92 0.726 1.35 1.05 1.96 1.52 2.72 2.19

7.8 1.16 0.912 1.69 1.32 2.45 1.90 3.39 2.74

7.9 1.46 1.15 2.12 1.66 3.06 2.39 4.24 3.43

8.0 1.83 1.44 2.65 2.07 3.83 2.98 5.28 4.28

8.1 2.29 1.80 3.32 2.60 4.77 3.73 6.55 5.32

8.2 2.86 2.26 4.14 3.25 5.94 4.65 8.11 6.61

8.3 3.58 2.83 5.16 4.06 7.36 5.78 10.00 8.18

8.4 4.46 3.54 6.41 5.05 9.09 7.17 12.27 10.10

8.5 5.55 4.41 7.98 6.28 11.18 8.87 14.97 12.40

Freshwater data from Trussel (1972).

Seawater values from Bower and Bidwell (1978). Salinity for Seawater values = 34% at an

ionic strength of 0.701m.

EXAMPLE:

A fresh water sample sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as Total Ammonia Nitrogen.

1. The percentage from the table is 11.18% (or 0.1118).

2. 1.0 ppm Total Ammonia Nitrogen x 0.1118 = 0.1118 ppm Unionized Ammonia Nitrogen.

3. Total Ammonia-Nitrogen 1.0000 ppm Unionized Ammonia-Nitrogen - 0.1118 ppm Ioinized Ammonia-Nitrogen = 0.8882 ppm

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