LaMotte AB-HCM User Manual

MAG NE SIUM & MAN GA NESE

WARNING! This set contains chemicals

that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision

SOIL TESTS

MODULE C

MODEL AM-32 · CODE 5938-01

QUANTITY CONTENTS CODE

30 mL *Magnesium Test Solution #1 *5140-G
30 mL *Manganese-Magnesium Solution #2 *5145WT-G
10 g Manganese Buffer Reagent 6310-D
15 g *Manganese Periodate Reagent *6311-E
1 Filter Paper, 50/pk 0465-H
2 Spot Plates, double, plastic 0159
2 Test Tubes, 1-8 mL, plastic, w/caps 0755
2 Plastic Rods, stirring 0519
1 Spoon, 0.5 g 0698
1 Test Tube, plastic, filtrate 0749
1 Funnel, plastic 0459
1 Color Chart, Magnesium in Soil 1306
1 Color Chart, Manganese in Soil 1307-01
1 Pipet, plain, plastic 0364
1 Pipet, plain, glass w/cap 0371
2 Spoons, 0.05 g 0696

*WARNING: Reagents marked with a * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these
reagents see MSDS CD or www.lamotte.com. To obtain a printed copy,
contact LaMotte by email, phone or fax.

To order individual reagents or test kit components, use the specified code
number.

EX TRAC TION PRO CE DURE

1. Fill a test tube (0755) to line 7 with *Universal Extracting Solution
(5173).

2. Use the 0.5 g spoon (0698) to add four level measures of soil to the
test tube. Cap and shake for one minute.

NOTE: When adding samples with high concentrations of
carbonates to *Universal Extracting Solution (5173), swirl tube to
mix for 30 seconds before capping to allow gases to escape.

3. Fold a piece of filter paper (0465) in half, then in half again. Press
corners together to form a cone. Place in funnel (0459). Pour
extract solution into funnel, collecting extract. This clear extract is
used for the test.

MAG NE SIUM TEST

1. Use a transfer pipet (0364) to add ten drops of soil extract to a
large depression on a spot plate (0159).

2. Add one drop of *Magnesium Test Solution (5140). Stir with a
stirring rod (0519). Solution will turn pale yellow.

3. Add one drop of *Manganese-Magnesium Solution #2 (5145WT).
Stir with a stirring rod. Compare to color standards on Magnesium
in Soil Color Chart (1306). Continue adding *Manganese­Magnesium Test Solution #2 one drop at a time until sample color
matches a color standard. Record as ppm Magnesium.

NOTE: It usually requires 2 drops of *Manganese-Magnesium Test
Solution #2 to cause color change.

IN TER PRE TA TION OF MAG NE SIUM TEST

Soils giving a very low test should be treated with dolomitic lime or
fertilizers, such as double-manure salts, which contain considerable
magnesia. Soils giving high, or very high, magnesium tests, with low
calcium tests, should receive applications of gypsum or high calcic lime,
in order to prevent calcium deficiency, due to over-balance of
magnesium.

MAN GA NESE TEST

1. Use a transfer pipet (0364) to add 10 drops of soil extract to the
large depression on a spot plate.

2. Use the 0.05 g spoon (0696) to add one measure of Manganese
Buffer Reagent (6310). Mix with a clean stirring rod (0519) until
the powder dissolves.

3. Use the other 0.05 g spoon (0696) to add one measure of
*Manganese Periodate Reagent (6311). Mix with a clean stirring
rod for 20 seconds.

NOTE: The *Manganese Periodate Reagent will not dissolve
completely.

4. Match the color in the spot plate to a color standard on the
Manganese in Soil Color Chart (1307-01). Record as ppm
Manganese.

NOTE: Immediately clean the spot plate to prevent staining.

IN TER PRE TA TION OF MAN GA NESE TEST

Manganese occurs in small amounts in all soils, chiefly in insoluble
combinations. In some calcareous soils and acid soils, which have been
heavily limed, practically no manganese is present in active forms, and
some crops are unable to obtain even the small amount necessary to
meet their requirements. Poor growth and a yellow, chlorotic condition
results.

On the other hand, strongly acid soils may contain injurious
concentrations of active manganese compound. Under some conditions
liming is a corrective measure.

Manganese is changed by oxidation to less active forms, or may be
leached from the soil. Hence, tests are of most significance when made
just prior to planting, or during crop growth. A negative test at such
time indicates the desirability of applying manganese. Twenty-five
pounds of commercial manganese sulfate per acre is usually adequate to
correct any possible deficiency. It is doubtful if manganese is needed if
any positive test whatever is developed. Medium, or low, tests are of
little significance, except as indicating no manganese deficiency. High,
or very high tests are undesirable and indicate a need for lime.

LaMOTTE COM PANY

Helping Peo ple Solve An a lyt i cal Challenges

PO Box 329 • Chestertown • Mary land • 21620 • USA

800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax [phone]

Visit us on the web at www.lamotte.com

®

65938-C · 10.11