
60 mL Standard Turbidity Reagent 7520-H
2 Turbidity Columns 0835
1 Brush, Test Tube 0513
1 Pipet, 0.5 mL, plastic, w/cap 0369
1 Rod, plastic, stirring 1114
*WARNING: Reagents marked with an * are considered to be potential health hazards.
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digit reagent code number listed on the reagent label, in the contents
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WARNING! This set contains chemicals that
may be harmful if misused. Read cautions on
individual containers carefully. Not to be used
by children except under adult supervision.
This test is performed by comparing the turbidity of a measured amount of the sample
with an identical amount of turbidity-free water containing a measured amount of
standardized turbidity reagent. The readings are made by looking down through
the column of liquid at a black dot. If turbidity is present, it will interfere with the
passage of light through the column of liquid. Small amounts of turbidity will cause
a “blurring” of the black dot in the bottom of the tube. Large amounts of turbidity
looking down through the column. Any color that may be present in the sample should
be disregarded. This determination is concerned only with the haziness or cloudy
nature of the sample.
1. Fill one Turbidity Column (0835) to the 50 mL line with the sample water. If the
black dot on the bottom of the tube is not visible when looking down through the
2. Fill the second Turbidity Column (0835) with an amount of turbidity-free water
that is equal to the amount of sample being measured. Distilled water is preferred;
however, clear tap water may be used. This is the “clear water” tube.
3.
is equally clear in both tubes, the turbidity is zero. If the black dot in the sample
tube is less clear, proceed to Step 4.
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4. Shake the Standard Turbidity Reagent (7520) vigorously. Add 0.5 mL to the “clear
water” tube. Use the stirring rod (1114) to stir contents of both tubes to equally
distribute turbid particles. Check for amount of turbidity by looking down through
the solution at the black dot. If the turbidity of the sample water is greater than
that of the “clear water”, continue to add Standard Turbidity Reagent in 0.5 mL
increments to the “clear water” tube, mixing after each addition until the turbidity equals that of the sample. Record total amount of Standard Turbidity Reagent
added.
5. Each 0.5 mL addition to the 50 mL size sample is equal to 5 Jackson Turbidity
Units (JTUs). If a 25 mL sample size is used, each 0.5 mL addition of the
Standard Turbidity Reagent is equal to 10 Jackson Turbidity Units (JTUs). See the
table below. Rinse both tubes carefully after each determination.
1 0.5 5 JTU 10 JTU
2 1.0 10 JTU 20 JTU
3 1.5 15 JTU 30 JTU
4 2.0 20 JTU 40 JTU
5 2.5 25 JTU 50 JTU
6 3.0 30 JTU 60 JTU
7 3.5 35 JTU 70 JTU
8 4.0 40 JTU 80 JTU
9 4.5 45 JTU 90 JTU
10 5.0 50 JTU 100 JTU
15 7.5 75 JTU 150 JTU
20 10.0 100 JTU 200 JTU
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