
TO TAL, CAL CIUM & MAG NE SIUM
HARD NESS TEST KIT
CODE 4824-LT-01
QUANTITY CONTENTS CODE
15 mL *Sodium Hydroxide Reagent
w/Metal Inhibitors
50 Calcium Hardness Indicator Tablets T-5250-H
15 mL *Hardness Reagent # 5 *4483-E
100 Hardness Reagent # 6 Tablets 4484-J
60 mL Hardness Reagent # 7 4487WT-H
1 Test Tube, w/cap 4488
1 Pipet, 0.5 mL, plastic 0353
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these
reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by
e-mail, phone or fax.
To order individual reagents or test kit components, use the specified code
number.
*4259-E

TEST PRO CE DURES
TOTAL HARDNESS
1. Fill the test tube (4488) to the desired line with sample water.
To receive results in ppm, fill tube to upper line.
To receive results in gpg, fill tube to middle line.
If the hardness level is above 200 ppm, fill tube to lower line.
NOTE: 1 gpg = 17.1 ppm
2. Add five drops of *Hardness Reagent #5 (4483). Swirl to mix.
3. Add one Hardness Reagent #6 Tablet (4484). Swirl to dissolve tablet.
Solution will turn red if hardness is present. If solution is blue, there is
no measurable amount of hardness.
4. While gently swirling tube, add Hardness Reagent #7 (4487WT), one
drop at a time until color changes from red to clear blue. Count the
number of drops added. Hold dropper bottle vertically.
5. To determine total hardness test result, multiply the number of drops
added in Step 4 by:
lower line 20 ppm CaCO
middle line 1 gpg CaCO
upper line 10 ppm CaCO
3
3
3
CALCIUM HARDNESS TEST
1. Fill test tube (4488) to the desired line (see Step 1, above) with
sample water.
2. Add 6 drops of *Sodium Hydroxide Reagent w/Metal Inhibitors
(4259). Swirl to mix.
3. Add one Calcium Hardness Indicator Tablet (T-5250). Swirl to
dissolve tablet. Solution will turn red if hardness is present. If solution
is blue, there is no measurable amount of hardness.
4. While gently swirling tube, add Hardness Reagent #7 (4487WT), one
drop at a time until color changes from red to clear blue. Count the
number of drops added. Hold dropper bottle vertically.
5. To determine calcium hardness test result, multiply the number of
drops added in Step 3 by:
lower line 20 ppm CaCO
middle line 1 gpg CaCO
upper line 10 ppm CaCO
3
3
3

ANAL Y SIS OF HARD NESS IN SALT WA TER
When sea and estuarine waters containing very high levels of mineral salts
are to be tested, the sample must be diluted to a practical concentration
before titration. This test is supplied with a calibrated pipet for performing
the simple, convenient dilutions described below.
TOTAL HARDNESS DILUTION (1 TO 17.2)
1. Use the 0.5 mL pipet (0353) to transfer 0.5 mL of the salt water to be
tested to the test tube (4488).
2. Fill tube to 10 ppm line with distilled water.
3. Follow Steps 2 through 5 under the Total Hardness test procedure.
Multiply the number of drops by 172 to obtain the test result
expressed as Total Hardness in ppm CaCO3.
CALCIUM HARDNESS DILUTION (1 TO 8.6)
1. Use the 0.5 mL pipet (0353) to transfer 1.0 mL (two measures) of the
salt water to be tested to the test tube (4488).
2. Fill tube to 10 ppm line with distilled water.
3. Follow Steps 2 through 4 under Calcium Hardness test procedure.
4. Multiply the number of drops by 86 to obtain the test result expressed
as Calcium Hardness in ppm CaCO3.
NOTE: Calcium Hardness test result may be converted to ppm
Calcium Chloride (CaCl2) by means of the following formula:
ppm CaCl2 = ppm CaCO3 x 1.11
MAGNESIUM HARDNESS OF SALT WATER
Magnesium Hardness = Total Hardness - Calcium Hardness
Magnesium Hardness test result may be converted to ppm Magnesium
Chloride (MgCl2) by means of the following formula:
ppm MgCl2 = CaCO3 x 0.95

LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Chal lenges
PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
SM
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