Page 1
TO TAL, CAL CIUM & MAG NE SIUM
L
Mott
WARNING! This set contains chemicals
that may be harmful if misused. Read
cautions on individual containers
carefully. Not to be used by children
except under adult supervision
HARD NESS TEST KIT
CODE 4824-DR-LT
QUANTITY CONTENTS CODE
15 mL *Sodium Hydroxide Reagent
w/Metal Inhibitor
50 Calcium Hardness Indicator Tablets T-5250-H
15 mL *Hardness Reagent #5 *4483-E
100 Hardness Reagent #6 Tablets 4484-J
60 mL Hardness Reagent #7 4487DR-H
1 Test Tube, 5-10-12.9-15-20-25 mL,
glass, w/cap
1 Direct Reading Titrator, 0-200 Range 0382
1 Pipet, 0.5 mL, plastic 0353
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these
reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by
e-mail, phone or fax.
To order individual reagents or test kit components, use the specified code
number.
NOTE: Read Direct Reading Titrator Manual before proceeding. The
Titrator is calibrated in terms of hardness expressed as parts per million (ppm)
Calcium Carbonate as CaCO3. Each minor division on the Titrator scale
equals 4 ppm CaCO3.
*4259-E
0608
Page 2
TOTAL HARDNESS TEST PROCEDURE
If the plunger reaches the bottom line on the scale
(200 ppm) before the endpoint color change occurs, refill
Read the test
result directly
from the scale
where the large
ring on the
Titrator meets the
Titrator barrel.
Record as ppm
Total Hardness as
(CaCO ).
3
7.
NOTE:
the Titrator and continue the titration. When recording the test result,
be sure to include the value of the original amount of reagent dispensed
(200 ppm).
Parts per million CaCO may be converted to grains per gallon (gpg)
3
CaCO :
3
gpg CaCO = ppm CaCO x 0.058
33
EXAMPLE:
Plunger tip is
2 minor divisions
below line 80.
Test result is:
80 + (2 divisions x 4)
= 88 ppm.
80
100
Result:
88 ppm
120
2.
5.
Fill
Direct Reading
Titrator (0382)
with Hardness
Reagent #7
(4487DR).
Insert the
Titrator into
the center
hole of the
test tube cap.
1.
3.
4.
tube, slowly press
the plunger to
titrate until the
red color changes
to clear blue.
Add 5 drops
of *Hardness
Reagent #5
(4483).
Swirl to mix.
Add one Hardness
Reagent #6 Tablet
(4484). Cap and swirl
Fill a test
tube (0608)
to the 12.9
mL line with
the sample
water.
until tablet disintegrates.
Solution will turn red if
hardness is present.
If solution is blue,
there is no
measurable
amount of
hardness.
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
While gently
swirling the
6.
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
Page 3
CALCIUM HARDNESS TEST PROCEDURE
If the plunger reaches the bottom line on the scale
(200 ppm) before the endpoint color change occurs,
NOTE:
refill the Titrator and continue the titration.
When recording the test result, be sure to include the value of the
original amount of reagent dispensed (200 ppm).
MAGNESIUM HARDNESS TEST PROCEDURE
Subtract Calcium Hardness from Total Hardness. Record as ppm
Magnesium Hardness as CaCO .
3
Magnesium Hardness (ppm CaCO ) =
3
_
Total Hardness Calcium Hardness
Read the test
result directly
from the scale
where the large
ring on the
Titrator meets the
Titrator barrel.
Record as ppm
Total Hardness as
(CaCO ).
3
7.
EXAMPLE:
Plunger tip is
2 minor divisions
below line 80.
Test result is:
80 + (2 divisions x 4)
= 88 ppm.
80
100
Result:
88 ppm
120
2.
5.
Fill
Direct Reading
Titrator (0382)
with Hardness
Reagent #7
(4487DR).
Insert the
Titrator into
the center
hole of the
test tube cap.
1.
3.
4.
tube, slowly press
the plunger to
titrate until the
red color changes
to clear blue.
Add 6 drops
of *Sodium
Hydroxide
w/Metal
Inhibitor
(4259). Cap
and swirl to
mix.
Add one
Calcium
Hardness Indicator
Tablet (T-5250).
Cap and swirl
until tablet
disintegrates.
Solution will turn
red.
Fill a test
tube (0608)
to the 12.9
mL line with
the sample
water.
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
While gently
swirling the
6.
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
Page 4
ANAL Y SIS OF HARD NESS IN SALT WA TER
When sea and estuarine waters containing very high levels of mineral salts are
to be tested, the sample must be diluted to a feasible concentration before
titration. This test is supplied with a calibrated pipet for performing the kit
dilutions described below.
TOTAL HARDNESS DILUTION (1 TO 25.8)
1. Use the 0.5 mL pipet (0353) to transfer 0.5 mL of the salt water to be
tested to the test tube (0608).
2. Dilute to the 12.9 mL line with distilled water.
3. Follow Steps 2 through 7 under the Total Hardness Test Procedure. Multiply
Titrator reading by 25.8. Record as ppm Total Hardness as CaCO
3.
CALCIUM HARDNESS DILUTION (1 TO 12.9)
1. Use the 0.5 mL pipet (0353) to transfer 1.0 mL (two measures) of the salt
water to be tested to the test tube (0608).
2. Dilute to the 12.9 mL line with distilled water.
3. Follow Steps 2 through 7 under Calcium Hardness test procedure. Multiply
Titrator reading by 12.9. Record as ppm Calcium Hardness as CaCO
3.
4. To convert Calcium Carbonate to Calcium Chloride, multiply by 1.11.
Record as ppm Calcium Chloride.
ppm CaCl2 = ppm CaCO3 x 1.11
5. To convert Calcium Carbonate to Calcium, multiply by 0.4. Record as
ppm Calcium.
ppm Ca = ppm CaCO3 x 0.4
MAGNESIUM HARDNESS OF SALT WATER
Subtract Calcium Hardness from Total Hardness. Record as ppm Magnesium
Hardness as CaCO3.
Magnesium Hardness (ppm CaCO
) = Total Hardness - Calcium
3
Hardness
To convert Magnesium Hardness as CaCO3 to Magnesium Chloride, multiply
by 0.95. Record as ppm Magnesium Chloride.
ppm MgCl2 = ppm CaCO3 x 0.95
To convert Magnesium Hardness to Magnesium, multiply by 0.24. Record as
ppm Magnesium.
ppm Mg = ppm CaCO3 x 0.24
LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Chal lenges
PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
®
8/11
Loading...