LaMotte 4533 User Manual

TURF LAB SOIL TEST KIT

MODEL TL-2 · CODE 5414

QUANTITY CONTENTS CODE

250 mL *Acid Extracting Solution *6361-K
2 x 60 mL Charcoal Suspension 5638-H
30 mL *Sodium Hydroxide, 15% *7886WT-G
500 mL Tricon Flocculating Solution 5941-L
30 mL *Wide Range Indicator *2218-G
250 mL *Mixed Acid Reagent *V-6278-K
10g *Nitrate Reducing Reagent *V-6279-D
60 mL *VM Phosphate *4410-H
15 mL *Reducing Reagent *6405-E
60 mL *Special NF Phosphorus Extracting Solution *6362-H
120 mL *Potassium TPB Solution *3825-J
15 mL *Iron Reagent #1 *4450-E

4.5g *Iron Reagent #2 Powder *4451-S
30 mL *Calcium-Magnesium Buffer *5126-G
15 mL *CM Indicator Reagent *6522-E
2 x 60 mL Standard EDTA Reagent 5254-H
30 mL Calcium Magnesium Inhibitor Reagent 3922-G
15 mL *TEA Reagent *3921-E
15 mL *Inhibitor Solution *9258-E
15 mL *Sodium Hydroxide Reagent w/Metal Inhibitors *4259-E
100 Calcium Hardness Indicator Tablets T-5250-J
60 mL Texture Flocculating Reagent 5643PS-H
60 mL *Texture Dispersing Reagent *5644PS-H
4 Test Tubes, 5-10-15 mL, plastic, w/cap 0701
8 Test Tubes, plastic, w/caps 0106
1 Test Tube, 5-10-15 mL, glass, w/cap 0778

1 Direct Reading Titrator 0384
3 Soil Texture Tube 0760
1 Double Tube, Potassium 0796
1 Test Tube Brush 0514
1 Pipet, transfer, plastic 0364
2 Pipets, 0.5 mL, plastic 0353
1 Pipet, plain, plastic 0352
5 Pipets, 1.0 mL, plastic 0354
2 Pipets, 1.0 mL, plastic w/cap 0372
1 Spoon, 0.1g, plastic 0699
1 Spoon, 1.0g, plastic 0697
1 Spoon, 0.5g, plastic 0698
1 Spoon, 0.05g, plastic 0696
1 Demineralizer Bottle 1155
2 Amber Bottles 0990
1 Funnel, plastic 0459
1 Filter Paper, 9 cm, 100/pk 0465
1 Test Paper, pH, 4.5-7.5 2953
1 Beaker, 50 mL, plastic 0944
1 Soil Texture Stand 1053
1 Graduated Cylinder, 25 mL 0417
1 Graduated Cylinder, glass, 100 mL 0419
1 Soil Measure, 15g 1165
1 pH Wide Range Octa-Slide Bar, 4.5-8.0 3424
1 Nitrate Nitrogen Octa-Slide Bar, 2.5-100 ppm 3422
1 Iron Octa-Slide Bar, 2.5-50 ppm 3479
1 Phosphorus Octa-Slide Bar, 15-150 ppm 3423
1 Octa-Slide Viewer 1100
1 Greenskeeper Soil Sampler 1159
1 Dial Thermometer 1116
100 Soil Sample Bags 0615

1 Dispenser Cap 0693

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2 Soil Test Report Form, 50/pad 1626
1 A Study of Soil Science 1530
1 LaMotte Soil Handbook 1504

*WARNING: Reagents marked with an * are considered to be potential
health hazards. To view or print a Material Safety Data Sheet (MSDS) for
these reagents go to www.lamotte.com. To obtain a printed copy, contact
LaMotte by e-mail, phone or fax.

To order a complete set of refill reagents, order as R-5414. To order individual
reagents or test components, use the specified code number.

USE OF THE OCTA-SLIDE VIEWER

The Octa-Slide Viewer should be held so non-direct light
enters through the back of the viewer. With sample tube
inserted at top, slide the Octa-Slide bar through the
viewer and match with color standard.

USE OF THE GREENSKEEPER SOIL SAM PLER

Pass the solid brass rod through the holes in the hollow brass tube to form a
handle. Brush away grass or leaves from the area to be sampled. Holding the
handle, push the hollow tube firmly, with a twisting motion, into the soil to the
desired depth. Pull the tube straight up to remove it from the soil. Remove the
solid rod from the sampler. Insert the solid rod into the tube and firmly push
out of the soil sample.

TEM PER A TURE

1. Remove the dial thermometer from the protective plastic sheath.

2. Firmly push the thermometer into the soil to a depth of 2 to 3 inches.

3. Read the temperature from the scale when it stablizes.

3

EX TRAC TION

The following method of extraction is used for obtaining the soil filtrate for the
tests for nitrogen, phosphorus, potassium, calcium, magnesium and iron. A
separate extraction is required for pH. Consult the LaMotte Soil Handbook for
information on sampling and preparation of the soil for testing.

Read the Demineralizer Bottle Instructions before proceeding.
NOTE: The Single Test Procedure will give an adequate amount of soil extract

to do one test. The extraction procedure using 15 grams of soil should be used
when testing for more than one factor.

MUL TI PLE TEST PRO CE DURE

1. Use the 1 mL pipet (0354) to add 5 mL (5 x 1 mL) of the *Acid

Extracting Solution (6361) to the 100 mL graduated cylinder (0419).
Dilute to 75 mL with deionized water.

2. Pour this solution into the 100 mL bottle (0990).

3. Measure 15 g of the soil sample and add to the solution in the bottle. (Use

the plastic soil measure (1165) which is filled level full with the soil
sample.) Add 2 mL of Charcoal Suspension (5638). Cap and shake to mix
for 5 minutes.

4. Use the funnel (0465) and filter paper (0465) to filter solution. Collect

the soil filtrate in a 100 mL bottle. The soil filtrate is used for all of the
tests, except pH.

SIN GLE TEST PRO CE DURE

1. Use the 1 mL pipet (0354) to add 1 mL of the *Acid Extracting Solution

(6361) to the test tube (0701). Dilute to 15 mL line with deionized water.

2. Use the 1.0 g spoon (0697) to add 3 measures of soil.

3. Add 0.5 mL of Charcoal Suspension (5638). Cap and shake for 5 minutes.

4. Use the funnel (0465) and filter paper (0465) to filter solution. Collect

the soil filtrate in a 100 mL bottle. The soil filtrate is used for all of the
tests, except pH.

pH

1. Fill a test tube (0106) to the 5 mL line with Tri c o n Flocculating Solution

(5941).

2. Use the 0.5 g spoon (0698) to add 3 measures of the soil sample. Stopper

the tube and gently mix by inverting the tube back and forth slowly for
one minute. Allow the soil particles to settle.

3. Use the pipet (0364) to transfer the clear solution above the soil to

another tube (0106). Fill second tube to the 25 mL line.

4. Add 6 drops of *Wide Range Indicator (2218). Cap and mix.

5. Insert test tube into the Octa-Slide Viewer (1100). Slide the pH Wide

Range Octa-Slide Bar (3424) into the Viewer. Match the sample color to a
color standard. Record as pH. If the sample color falls between two
standards, take the midpoint between the two standard values as the result.
NOTE: Liming Tables are found on page 23 of the LaMotte Soil Handbook.

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NI TRATE NI TRO GEN

NOTE: Place Dis penser cap (0693) on *Mixed Acid (V-6278). Save Dis penser
Cap for re agent re fills.

1. Fill test tube (0106) to the 5 mL line with the soil filtrate.

2. Dilute to 10 mL line with *Mixed Acid Reagent (V-6278).

3. Use the 0.1 g spoon (0699) to add 2 level measures of *Nitrate Reducing

Reagent (V-6279). Cap and mix by inverting 50 - 60 times in one minute.
Wait 10 minutes for maximum color development.
NOTE: At the end of the waiting period, an undissolved portion of the
*Nitrate Reducing Reagent may remain in the bottom of the tube without
affecting the results.

4. Mix the sample one time and insert the tube into the top hole of the

Octa-Slide Viewer (1100). Slide the Nitrate Nitrogen Octa-Slide Bar
(3422) into the Viewer. Match sample color to a color standard. Record as
lb/acre nitrate nitrogen. If the sample color falls between two standards,
take the midpoint between the two standard values as the result.
NOTE: Values above the highest standard may be determined by diluting
the filtrate and multiplying the results by a dilution factor.

PHOS PHO RUS

1. Use the 1 mL pipet (0354) to add 1 mL of the soil filtrate to the tube

(0106).

2. Dilute to the 5 mL line with deionized water.

3. Use the 0.5 mL pipet (0353) to add 0.5 mL of *VM Phosphate (4410).

Cap and mix by inverting 3 times. Wait 5 minutes.

4. Use the plain pipet (0352) to add 2 drops of *Reducing Reagent (6405).

Cap and mix. Solution will turn blue.

5. Insert test tube into the Octa-Slide Viewer (1100). Slide the Phosphorus

Octa-Slide Bar (3423) into the Viewer. Match sample color to a color
standard. Record as lb/acre phosphorus. If the sample color falls between
two standards, take the midpoint between the two standard values as the
result.
NOTE: Values above the highest standard may be determined by diluting
the filtrate and multiplying the results by a dilution factor.

PHOS PHO RUS IN AL KALINE SOILS

A special extraction procedure is used for determining the available phosphorus
content of Western U.S. soils where the pH value is above 7.0.

EX TRAC TION PRO CE DURE

1. Use the 1 mL pipet (0354) to add 1 mL of the *Special NF Extracting

Solution (6362) to the test tube (0701). Dilute to 15 mL line with
deionized water.

2. Use the 1 g spoon (0697) to add 3 measures of soil.

3. Add 0.5 mL of Charcoal Suspension (5638). Cap and mix for 5 minutes.

5.

4. Use the funnel (0459) and filter paper (0465) to filter solution. Collect

the soil filtrate in a vial (0989).

5. Perform the Phosphorus test according to the procedure above.

PO TAS SIUM

1. Use the 1 mL pipet (0354) to add 2 mL of soil filtrate to the round tube.

2. Use a second 1 mL pipet (0354) to add 2 mL of *Potassium TPB Solution

(3825). Wait 5 minutes.

3. Dilute to top line with deionized water. Cap and shake to mix.

4. Remove the cap and slowly insert the square tube with the collar. The

square tube will slide up and down through the collar and will fill with
liquid.

5. Viewing from above, adjust the square tube in the turbid solution until the

black dot on its base is obscured. Hold the round tube at the top to avoid
blocking the light.

6. Note the height of the liquid in the square tube. Record as lb/acre

potassium. To convert the results to potash (K2O), multiply the result by

1.2. Record as lb/acre potash.
NOTE: Brush tubes thoroughly after each use.

NEU TRAL IZA TION OF SOIL FIL TRATE

In the Iron, Calcium and Magnesium tests, the acidity of the soil filtrate must
be neutralized before testing. This is done by adding Sodium Hydroxide, 15%
(7886) to the soil filtrate, one drop at a time, until the pH test paper (2953)
indicates that the pH is between 6.0 and 7.0.

IRON

1. Fill test tube (0106) to the 5 mL line with soil filtrate.

2. Neutralize according to Neutralization of Soil Filtrate Instructions.

3. Add 5 drops of *Iron Reagent #1 (4450).

4. Use the 0.05 g spoon (0696) to add one measure of *Iron Reagent #2

Powder (V-4451). Cap and mix until the powder has dissolved. Wait 3
minutes for maximum color development.

5. Mix once and insert test tube into the Octa-Slide Viewer (1100). Slide the

Iron Octa-Slide Bar (3479) into the Viewer. Match sample color to a color
standard. Record as ppm iron. If the sample color falls between two
standards, take the midpoint between the two standard values as the result.

6

CAL CIUM AND MAG NE SIUM

The Schwarzenbach EDT A titration method, used to determine calcium and
magnesium, involves two titrations. The first titration gives the combined
calcium and magnesium content and the second gives the calcium content.
Magnesium is calculated from the difference between the titration values.

Read LaMotte’s Direct Reading Titrator Manual before proceeding.

DI LU TION OF SOIL EX TRACT

Using the 25 mL graduated cylinder (0417), measure 10 mL of the soil filtrate
and transfer it to a 50 mL beaker (0944). Add 10 mL of deionized water, mix
and neutralize according to the procedure previously described.

TI TRA TION A, CAL CIUM & MAG NE SIUM

1. Fill the test tube (0778) to the 5 mL line with diluted soil filtrate. Dilute

to the 10 mL line with deionized water.

2. Add 5 drops of Calcium Magnesium Inhibitor Reagent (3922). Wait 5

minutes. Swirl to mix.

3. Add 5 drops of *Calcium-Magnesium Buffer (5126). Swirl to mix.

4. Add 10 drops of *CM Indicator (6522). Swirl to mix. Solution will turn

red.

5. Fill the Direct Reading Titrator (0384) with Standard EDTA Reagent

(5254). Insert Titrator tip into the center hole of the test tube cap.

6. While gently swirling the tube, slowly press the plunger to titrate until red

color changes to blue.

7. Read the Titration scale at the tip of the plunger. Multiply by 5.16. Record

as Titration Value A.

TI TRA TION B, CAL CIUM

1. Fill test tube (0778) to the 5 mL line with diluted soil filtrate. Dilute to 10

mL line with deionized water.

2. Add 2 drops of *Inhibitor Solution (9258). Swirl to mix.

3. Add 2 drops of *TEA Reagent (3921). Swirl to mix.

4. Add 8 drops of *Sodium Hydroxide Reagent with Metal Inhibitors (4259).

Swirl to mix.

5. Add one Calcium Hardness Indicator Tablet (T-5250) to the test sample.

Cap and mix until tablet disintegrates. Solution will turn red.

6. Fill the Titrator with Standard EDTA Reagent (5254). Insert Titrator tip

into the center hole of the test tube cap.

7. While gently swirling the tube, slowly press the plunger to titrate until the

red color changes to a clear blue, and does not revert to red upon standing
1 - 2 minutes.

8. Read the Titrator scale at the tip of the plunger. Multiply by 5.16. Record

as Titration Value B.

7

FI NAL RE SULTS

Calcium Content = 0.4 x Titration Value B = ppm Ca

Magnesium Content = 0.24 (Value A - Value B) = ppm Mg

Multiply the results by 2 to obtain the content in pounds per acre

EXAMPLE:

Titration Value A is 640 ppm CaCO
Titration Value B is 520 ppm CaCO

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3

Calcium = 0.4 x 520 = 208 ppm Ca

208 x 2 = 416 lb/acre Ca

Magnesium = 0.24 (640 - 520)

0.24 x 120 = 29 ppm Mg
29 x 2 = 58 lb/acre Mg

LaMOTTE COM PANY

Helping Peo ple Solve An a lyt i cal Chal lenges

PO Box 329 · Chestertown · Mary land · 21620 · USA

800-344-3100 · 410-778-3100 (Out side USA) · Fax [phone]

Visit us on the web at www.lamotte.com

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