
15 mL *Hardness Reagent #5 *4483-E
50 Hardness Reagent #6 Tablets 4484A-H
60mL Hardness Reagent #7 4487DR-H
1 Test Tube, 5-10-12.9-15-20-25 mL, glass, w/cap 0608
1 Direct Reading Titrator, 0-200 Range 0382
1 Pipet, 0.5 mL, plastic 0353
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the contents
list or in the test procedures. Omit any letter that follows or precedes the four digit code
number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy,
contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
NOTE: Read the Direct Reading Titrator Instruction Manual before proceeding.
The Titrator is calibrated in terms of total hardness expressed as parts per million
(ppm) calcium carbonate (CaCO3). Each minor division on the Titrator scale equals
4 ppm CaCO3.
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1. Fill the test tube (0608) to 12.9 mL line with sample water.
2.
3. Add one Hardness Reagent #6 Tablet (4484A). Cap and swirl until tablet
disintegrates. Solution will turn red if hardness is present. If solution is blue there
is no measurable amount of hardness.
4. Fill the Direct Reading Titrator (0382) with Hardness Reagent #7 (4487DR). Insert
Titrator in the center hole of the test tube cap.
5. While gently swirling the titration tube, slowly press the plunger to add Hardness
Reagent #7 (4487DR) until the red color changes to blue. Read the test result
directly from the scale where the large ring on the Titrator meets the Titrator barrel.
Record as ppm Total Hardness as CaCO3.
EXAMPLE: Plunger tip is 3 minor divisions below line 80.
Test result is 80 plus (3 divisions x 4) equals 92 ppm.
6. If the plunger tip reaches the bottom line on the Titrator scale (200 ppm) before the
the test result, be sure to include the value of the original amount of reagent
dispensed (200 ppm).
7. To convert ppm Hardness to grains per gallon (gpg), multiply by 0.058. Record as
gpg Hardness as CaCO3.
gpg CaCO3 = ppm CaCO3 x 0.058
When sea and estuarine waters containing very high levels of mineral salts are tested,
the sample must be diluted before titration. This test kit contains a calibrated pipet for
performing the dilution described below.
1. Use the 0.5 mL pipet (0353) to transfer 0.5 mL of the salt water sample to the test
tube (0608).
2. Dilute to the 12.9 mL line with distilled water (a 1 to 25.8 dilution).
3. Follow Steps 2 through 5 above. Multiply the Titrator reading by 25.8. Record as
ppm Total Hardness as CaCO3.
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