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1200 COLORIMETER
OZONE
MODEL 1200-OZ · CODE 3678-01
QUAN TITY CON TENTS CODE
15 mL Chlorine Inhibitor 3990-E
250 mL *Ozone Buffer *3991-K
30 mL Indigo Blue Stock Solution 3989-G
1 Bottle, HR Reagent, amber glass 0680-J
1 Sampling Apparatus 0681
1 Pipet, transfer, 1.0 mL 2-2170
1 Pipet, 5 mL, glass volumetric 0329
1 Pipet Pump, 10 mL 2-2216
1 Graduated Cylinder, 50 mL, glass 0418
1 Colorimeter Tubes, w/caps 0290-6
1 1200 Colorimeter for Ozone 26735
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
To order individual reagents or test kit components, use the specified code numbers.
IN TRO DUC TION
Ozone is sometimes used in place of, or in conjunction with, chlorine or other
halogens for disinfection of pool, spa or drinking waters. Recently, large aquatic
facilities have begun using ozone as a disinfectant in many artificial habitats.
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OZONE TEST PRO CE DURE - IN DIGO METHOD
Use the 1.0 mL
transfer pipet (2-
2170) and pipette
pump (2-2216) to
add 1.0 mL of HR
Reagent to each of
2 clean colorimeter
tubes (0967).
If chlorine is
present, add 3
drops Chlorine
Inhibitor (3990)
to each tube.
Cap tubes.
8.
5.
3. 4.
Use the 50 mL graduated
cylinder (0418) to carefully
add 45 mL of *Ozone Buffer
(3991) to amber glass bottle
marked HR Reagent (0680).
Use the 5 mL
volumetric pipet
(0329) and pipette
pump (2-2216) to add
5 mL of Indigo Blue
Stock Solution (3989)
to the amber glass
bottle. Cap and mix.
1. 2.
7.
Take one of the
prepared colorimeter
tubes and sampling
apparatus (0681) to
sampling site.
6.
Lower the end of the tubing
of sampling apparatus to
desired depth. Slowly
withdraw and depress
plunger several times to
purge syringe and tubing.
Slowly withdraw plunger to
fill purged syringe.
Remove plastic tubing
from syringe. Remove
Fill the second prepared
tube (0967) to the 10
mL line with ozone free
water. This is the
Reagent Blank.
HR Reagent
cap from the prepared tube.
Place tip of syringe against
inside of the prepared tube.
Slowly depress plunger and
fill to the 10 mL line and
cap. This is the Sample
Tube. NOTE: Do not shake
or invert the sample.
Read the 1200 Colorimeter Manual before proceeding.
Carefully wipe tubes dry before inserting into the
colorimeter chamber.
PROCEDURE
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Push the READ
button to turn the
meter on. Press the
ZERO button
and hold it for 2
seconds until bla is
displayed. Release
the button to take a
zero reading (0
ppm).
13. Note:
11.
9. 10.
12.
Wipe both tubes clean with a
lint-free cloth.
Insert the
Reagent Blank
into the
chamber, being
sure to align the
index line with
the arrow on
the meter.
Close the lid.
This tube is the
blank or zero.
Insert the
Sample Tube
into the
chamber, being
sure to align the
index line with
the arrow on
the meter.
Close the lid.
The HR Reagent must be made
fresh each week. If reagent is
refrigerated, it may be kept up to
3 weeks.
I200 COLORIMETER
• • • • • • • • • • • • • • • • • •
READ
bla
ZERO
Push the
READ
button. The
concentration
in ppm will be
displayed within
2 seconds.
ZERO
I200
COLORIIMETER
• • • • • • • • • • • • • • • • • •
READ
0.1 5
L
Mott
L
Mott
er2
NOTE: Zeroing the meter with sample water or an empty chamber will result in an
message when reading reacted samples. Meter must be zeroed with a reagent
blank.
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OZONE TEST METHOD SPEC I FI CA TIONS
APPLICATION
Drinking, pool, and aquatic waters.
RANGE
0.0 - 0.5 ppm Ozone
METHOD
Ozone rapidly and stoichiometrically decolorizes Indigo Trisulfonate under acidic
conditions.
HANDLING & PRESERVATION
Ozone is extremely unstable in aqueous solutions. Test must be performed
immediately and the sample must not be agitated.
INTERFERENCES
Manganese at any level will interfere.
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