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1200 COLORIMETER
EPA
COMPLIANTCOMPLIANT
FLUORIDE
SPADNS METHOD
MODEL 1200-FL · CODE 3674-01
QUANTITY CONTENTS CODE
2 x 100 mL *Acid-Zirconyl-SPADNS Reagent *3875-J
2 x 60 mL *Sodium Arsenite Solution *4128-H
1 Pipet, 0.5 mL, plastic 0353
1 Pipet, 1.0 mL, plastic 0354
1 Colorimeter Tubes, with caps, set of 6 0290-6
1 Water Sample Collecting Bottle 0688
1 1200 Colorimeter for Fluoride 26733
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specified code number.
NOTE: This procedure uses EPA approved SPADNS Reagent System for fluoride found
in method 4500-F-D, 18th Edfition of Standard Methods, page 1-27.
FLUORIDE INTRODUCTION
Fluoride may occur naturally in some ground waters or it may be added to public
drinking water supplies to maintain a 1.0 mg/L concentration to prevent dental cavities.
At higher concentrations, fluoride may produce an objectionable discoloration of tooth
enamel called fluorosis, though levels up to 8 mg/L have not been found to be
physiologically harmful.
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FLUORIDE TEST PROCEDURE - SPADNS METHOD
Insert the tube
into the
chamber, being
sure to align
the index line
with the arrow
on the meter.
Close the lid.
This tube is
the sample
blank.
5.
Use the 0.5 mL
pipet (0353) to
add 0.5 mL of
*Sodium Arsenite
Solution (4128).
Cap and mix.
3.
This test requires a
reagent blank.
Rinse a clean
colorimeter tube
(0290) with clear,
colorless, fluoride
free water. Fill to
the 10 mL line
with clear,
colorless, fluoride
free water.
2.
Fill the Water
Sample Collecting
Bottle (0688) with
sample water. This
will be used to
dispense sample
water for the tests.
1.
Use the 1.0 mL
pipet (0354) to
add 2 measures of
*Acid-Zirconyl
SPADNS
Reagent (3875).
Cap and mix
thoroughly. (This
is the reagent
blank.
4.
6.
Push the
READ button
to turn the meter
on. Press the
ZERO button
and hold it for 2
seconds until bla
is displayed.
Release the button
to take a blank
reading (0.0 ppm).
I200 COLORIMETER
• • • • • • • • • • • • • • • • • •
READ
ZERO
bla
L
Mott
Read the 1200 Colorimeter Manual before proceeding. Carefully
wipe tubes dry before inserting into the colorimeter chamber.
FLUORIDE
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FLUORIDE
9.
10 .
Align the index
line with the arrow
on the meter,
insert tube into
chamber. Close
the lid. Push the
READ button.
Record results as
ppm Fluoride.
CAL
I200COLORIMETER
• • • • • • • • • • • • • • • • • •
READ
18 . 0
Use the 1.0 mL
pipet (0354) to add
2 measures of
*Acid-Zirconyl
SPADNS Reagent
(3875). Cap and
mix thoroughly.
8.7.
Rinse a clean
colorimeter tube
(0290) with
sample water. Fill
to the 10 mL line
with sample
water.
Use the 0.5 mL
pipet (0353) to
add 0.5 mL of
*Sodium Arsenite
Solution (4128).
Cap and mix.
L
Mott
er2
NOTE: Zeroing the meter with sample water or an empty chamber will result in an
message. Meter must be zeroed with a reagent blank.
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FLUORIDE SPADNS TEST METHOD SPECIFICATIONS
APPLICATION
Drinking and surface waters; domestic and industrial waters.
RANGE
0.0 to 2.0 ppm Fluoride
METHOD
Colorimetric test based upon the reaction between fluoride and zirconium dye lake. The
fluoride reacts with the dye lake, dissociating a portion of it into a colorless complex ion
and dye. As the fluoride concentration increases, the color produced becomes
progressively lighter.
HANDLING & PRESERVATION
Samples may be stored and refrigerated in plastic containers.
INTERFERENCES
The following substances produce a positive interference at the concentration given:
Chloride (Cl-) 7000 mg/L
Phosphate (PO
-3
) 16 mg/L
4
Hexametaphosphate (NaPO3)61 mg/L
The following substances produce a negative interference at the concentration given:
Alkalinity (CaCO3) 5000 mg/L
Aluminum (Al+3) 0.1 mg/L
Iron (Fe+3) 10 mg/L
Sulfate (SO
-2
) 200 mg/L
4
Color and turbidity must be removed or compensated for in the procedure. Temperature
should be maintained within 5 degrees Celcius of room temperature.
10.10
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