LaMotte 3635-05 User manual

Salt Water
Aquaculture

Test Kit Instruction Manual

Code 3635-05

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This booklet provides step-by-step detailed instructions for the Code 3635-05 test kit.
It is important to review these instructions thoroughly
tests by the short-form instructions contained in the case lid.

To order individual reagents or test kit components, use the specifi ed code number.

before attempting to perform the

Safety / Testing Hints / Reagent Care .......................................... 4

Analytical Technique ..................................................................... 5

General Safety Information ........................................................... 6

Test Methods ................................................................................. 7

Test Procedures

Introduction ......................................................................................... 9

Alkalinity ............................................................................................. 10

Ammonia Nitrogen ............................................................................. 12

Carbon Dioxide .................................................................................. 15

Dissolved Oxygen .............................................................................. 16

Nitrate Nitrogen .................................................................................. 21

Nitrite Nitrogen ................................................................................... 22

pH ....................................................................................................... 23

Salinity ................................................................................................ 24

Kit Diagram ................................................................................... 26

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SAFETY/ TESTING HINTS / REAGENT CARE

*WARNING: Reagents marked with an * are considered to be potential health hazards.

Tightly close all
reagent
immediately after
use. Be sure not
to interchange caps
and pipets from
different containers.

Wipe up any reagent chemical spills,
liquid or powder, as soon as they
occur. Refer to label and SDS for
proper reagent disposal.

containers

Use care when dispensing or handling
all reagents. Some reagents also may
cause permanent stains if spilled.

Avoid prolonged
exposure of
equipment and reagents
to direct sunlight.
Protect reagents and
components from
extreme heat and cold.

4

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ANALYTICAL TECHNIQUE

1. Clean glassware is a must for accurate results.
Thoroughly rinse test tubes before and after
each test. Caps and stoppers should also be
cleaned after each use.

2. Use test tube caps or stoppers, not your fi ngers,
to cover test tubes and fl asks during shaking or
mixing.

3. When adding sample to calibrated test tube, be
sure vial is fi lled to the appropriate mark. The
bottom of the liquid (meniscus) should be level
with the desired mark. (Fig. 1)

4. When dispensing reagents from bottles fi lled
with dropper plug and cap, be sure to hold
bottle vertically and gently squeeze to dispense
the appropriate number of uniform drops.
(Fig. 2)

5. For those reagents to be added with the screwcap
pipet assemblies
on bottle and replace with the screwcap pipet.
NOTE: Place the polyseal caps back on the
reagent bottles for longer periods of storage. Be
sure that both pipet assemblies and polyseal caps
are thoroughly cleaned before placing on bottles
to avoid contamination.

6. When dispensing reagents from pipets, hold
pipet vertically to assure uniform drop size. This
is extremely important when performing drop
count titrations. (Fig. 3)

7. To fi ll pipets, squeeze rubber bulb and immerse
into reagent. Release bulb to fi ll. (Fig. 4)

8. To accurately dispense powdered reagents with
spoon, tap spoon on edge of reagent container
to remove excess reagent. (Fig. 5)

9. When performing tests that include Octa-Slide 2
Comparators, the comparator should be
positioned between the operator and non-direct
sunlight. This allows the light to enter through
the

light-diffusing screen at the back of the

comparator for optimum

enclosed, remove polyseal cap

color comparison.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

5

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GENERAL SAFETY PRECAUTIONS

1

12

Store the test kit in a
cool dry area.

3

34

Read the labels on
all reagent bottles.
Note warnings and
fi rst aid information.
Reagents marked with
a * are considered
possible health hazards.

5

Avoid contact between reagent
chemicals and skin, eyes, nose,
and mouth. Wear safety glasses when

2

4

6

Read all instructions and note
precautions before performing
the test procedure.
Read all Safety Data
Sheets (SDS). SDS

Safety

Data

are available at

Sheet

65

performing test procedures.

Keep all
equipment
and reagent
chemicals
out of the
reach of
young children.

7.

6

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In the event of an accident or suspected poisoning, immediately call
the Poison Center phone number in the front of your local telephone
directory or call a physician. Additional information for all LaMotte
reagents is available in the United States, Canada, Puerto Rico, and
the US Virgin Islands from Chem-Tel by calling 1-800-255-3924.
For other areas, call 813-248-0585 collect to contact Chem-Tel’s
International access number. Each reagent can be identifi ed by
the four digit number listed on the upper left corner of the
reagent label, in the contents list and in the test procedures.

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TEST METHODS

This test kit uses two basic analytical procedures common to fi eld test kits. A brief
explanation of each follows:

COLORIMETRIC: OCTA-SLIDE 2 VIEWER

In a visual colorimetric test, a sample is treated with reagent(s) to produce a color
reaction, generally in proportion to the amount of test factor present. The sample color
is then compared against color standards representing known concentrations of the
factor being tested over a specifi c range.

1

123

Insert Octa­Slide 2
into the Octa­Slide 2
(1101).

Bar
Viewer

2

Insert test
tube containing
reacted sample
into the Octa­Slide 2
(1101).

Viewer

3

Hold the
Octa-Slide 2
Viewer so that
non-direct light
enters through
the back of the
viewer.

4

4

Match sample
color to color
standard.
Record results.

Note:

NOTE: If sample color is between two standards, the
midpoint is taken as the result.

If the sample is darker than the highest standard, a
dilution may be performed on a fresh sample, and the
test repeated to bring the concentration within range.

DILUTIONS

The calibrated test tubes (0106) included in this kit may be used to perform dilutions
for the Ammonia Nitrogen and Nitrite Nitrogen tests. Distilled or deionized water is
needed to perform dilutions.
The following table provides a quick reference guide for dilutions of various
proportions. Once the dilution is prepared, use this diluted sample to perform the test,
and multiply the result by the dilution factor to obtain the actual concentration.

Sample Size Distilled Water

to Bring to 10 mL

5.0 mL 5.0 mL 2

2.5 mL 7.5 mL 4

Dilution Factor

7

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TITRIMETRIC: DIRECT READING TITRATOR

In a titrimetric method, titrating solution (or titrant) is added to a treated sample
until a color change occurs. The volume of titrant required to reach this endpoint is
proportional to the concentration of the factor being tested. Direct Reading Titrators
provide results directly in the appropriate concentration for the test—no counting of
drops, no calculations.

The Titrator consists of a plastic barrel, a plastic plunger, and a plastic adapter tip. The
adapter tip reduces the size of the drops that are dispensed, increasing the precision of
the test results. DO NOT the plunger or adapter tip from the Titrator.

123

Fill the test
tube to the
appropriate
line with
sample water.

4

To fi ll Titrator, slowly
withdraw the plunger
until the bottom of the
plunger is
opposite
the zero
mark on
the scale.

6

6

Insert the Titrator
into the center hole
of the test tube
cap. While gently
swirling tube, add
titrating solution
one drop at a time
until the desired
color changes occur.

Add reagents
as specifi ed
in the instructions
for the individual
test method. Cap
with the special test
tube cap. Mix by
swirling gently.

NOTE: If small air
bubbles appear in the
barrel, expel them by
partially fi lling the barrel
and pumping the titration
solution back into the
reagent container. Repeat
until bubble disappears.

7

7

Read the test
result directly
from the scale
where the large
ring on the Titrator
meets the Titrator barrel.

Depress the
Titrator plunger
to expel air. Insert
Titrator into the
plastic fi tting of
the titrating
solution bottle
and invert.

5

5

Turn the bottle
right-side-up
and remove the
Titrator.

8

8

When testing is
complete, discard
titrating solution
in Titrator.
Rinse Titrator
and titration tube
thoroughly.

8

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TEST PROCEDURES

INTRODUCTION

Proper control of water quality is an essential part of successful aquaculture operation.
Immediate test results provided by on-site water analysis equipment can confi rm a healthy
environment, or give early warning signals for required treatment.

1. Develop a routine testing schedule.

2. Keep records! Historical data is extremely important if treatments are required. Note
environmental conditions, fi sh activity, feeding habits, etc.

3. Observe fi sh to note any particular behavior or feeding rates, as this may be a sign of
stress.

4. Stable characteristics, such as alkalinity and hardness, do not have to be tested as
frequently as ones that fl uctuate, such as ammonia nitrogen, nitrite nitrogen, pH,
dissolved oxygen and temperature. Keep in mind that these factors fl uctuate throughout
the day and in some cases are interdependent.

5. Be alert to sudden changes in one factor, as it may be a clue to perform further analysis.

9

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Alkalinity

DESCRIPTION CODE

*BCG/MR Indicator *2311-PG-E
*Alkalinity Titration Reagent B *4493DR-H
Test Tube, 5-10-12.9-15-20-25 mL, glass, w/cap 0608
Direct Reading Titrator, 0-200 Range 0382

*WARNING: Reagents marked with an * are considered to be potential health hazards.
For futher information see page 4.

The Direct Reading Titrator is calibrated in terms of total alkalinity expressed as parts
per million (ppm) Calcium Carbonate (CaCO3). Each minor division on the Titrator
scale equals 4 ppm CaCO3.

10

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ALKALINITY TEST PROCEDURE

123

12 3

Fill the test
tube (0608)
to the 5 mL
line with the
sample water.

4

4

Fill Direct Reading
Titrator (0382)
with *Alkalinity
Titration Reagent B
(4493DR).

7

7

Read the test result
directly from the
scale where the large
ring on the Titrator
meets the Titrator barrel.
Record Total Alkalinity as ppm
Calcium Carbonate (CaCO3).

Add 4 drops
of *BCG-MR
Indicator
(2311-PG).

5

5

Insert the Titrator
into the center
hole of the test
tube cap.

8

NOTE: If the plunger tip reaches the
bottom line on the scale (200 ppm) before
the endpoint color change occurs, refi ll the
Titrator and continue the titration.

When recording the test result, be sure to
include the value of the original amount of
reagent dispensed (200 ppm).

Cap and
mix.
Solution
will turn
blue-green.

6

6

While gently
swirling the tube,
slowly press the
plunger to titrate
until blue-green
color changes
to pink.

11

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Ammonia Nitrogen

DESCRIPTION CODE

*Salicylate Ammonia #1 *3978L WT -G
*Salicylate Ammonia #2 *3979WT -G
Salicylate Ammonia #3 3982WT-G
Test Tube, 2.5-5-10 mL, plastic, w/cap 0106
Octa-Slide 2 Viewer 1101
Ammonia Nitrogen Octa-Slide 2 Bar, 0-2.0 ppm, Salt Water 3441-01-SW

*WARNING: Reagents marked with an * are considered to be potential health hazards.
For futher information see page 4.

12

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AMMONIA NITROGEN TEST PROCEDURE

12 3

4

Cap and mix.

7

Insert Ammonia
Nitrogen Octa­ Slide 2 Bar
(3441-01-SW)
into the
Octa-Slide 2

Viewer (1101).

Wait 1
minute.

1

MINUTE

5

8

Fill a test tube
(0106) to the
5 mL line
with the water
sample.

Add 7 drops
of *Salicylate
Ammonia #2
(3979WT).

Add 7 drops
of Salicylate
Ammonia #3
(3982WT).

Add 10 drops
of *Salicylate
Ammonia #1
(3978WT).

6

Cap and mix.

9

Cap and mix.

10

20

MINUTES

Wait 20

11

minutes.

Insert test
tube into
Octa-Slide 2
Viewer.

Calculations:

To express results as Ammonia (NH3):

Ammonia (NH

To express results as Ammonium (NH

Ammonium (NH

) = ppm Ammonia Nitrogen (NH3-N) x 1.2

3

+

) = ppm Ammonia Nitrogen (NH3-N) x 1.3

4

12

Match sample
color to a
color standard.
Record as ppm
Ammonia
Nitrogen.

+

):

4

13

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Ammonia in water occurs in two forms: toxic unionized ammonia (NH3) and the
relatively non-toxic form, ammonium ion (NH

+

). This test method measures both

4

forms as ammonia-nitrogen (NH3-N) to give the total ammonia-nitrogen concentration
in water. The actual proportion of each compound depends on temperature, salinity,
and pH. A greater concentration of unionized ammonia is present when the pH value
and salinity increase.

1. Consult the table below to fi nd the percentage that corresponds to the temperature,
pH and salinity of the sample.

2. To express the test result as ppm Unionized Ammonia Nitrogen (NH

-N), multiply

3

the total ammonia-nitrogen test result by the percentage from the table.

3. To express the test result as ppm Ionized Ammonia Nitrogen (NH

+

-N), subtract the

4

unionized ammonia nitrogen from the total ammonia-nitrogen.

10°C 15°C 20°C 25°C

pH FW1 SW2 FW SW FW SW FW SW

7.0 0.19 0.27 0.40 0.55

7.1 0.23 0.34 0.50 0.70

7.2 0.29 0.43 0.63 0.88

7.3 0.37 0.54 0.79 1.10

7.4 0.47 0.68 0.99 1.38

7.5 0.59 0.459 0.85 0.665 1.24 0.963 1.73 1.39

7.6 0.74 0.577 1.07 0.836 1.56 1.21 2.17 1.75

7.7 0.92 0.726 1.35 1.05 1.96 1.52 2.72 2.19

7.8 1.16 0.912 1.69 1.32 2.45 1.90 3.39 2.74

7.9 1.46 1.15 2.12 1.66 3.06 2.39 4.24 3.43

8.0 1.83 1.44 2.65 2.07 3.83 2.98 5.28 4.28

8.1 2.29 1.80 3.32 2.60 4.77 3.73 6.55 5.32

8.2 2.86 2.26 4.14 3.25 5.94 4.65 8.11 6.61

8.3 3.58 2.83 5.16 4.06 7.36 5.78 10.00 8.18

8.4 4.46 3.54 6.41 5.05 9.09 7.17 12.27 10.10

8.5 5.55 4.41 7.98 6.28 11.18 8.87 14.97 12.40

1

Freshwater data from Trussel (1972).

2

Seawater values from Bower and Bidwell (1978). Salinity for Seawater values = 34 ppt at an ionic strength

of 0.701 m.

FOR EXAMPLE:
A fresh water sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as Total
Ammonia-Nitrogen.

1. The percentage from the table is 11.18% (or 0.1118).

2. 1 ppm total Ammonia-Nitrogen x 0.1118 = 0.1118 ppm Unionized Ammonia­Nitrogen

3. Total Ammonia-Nitrogen 1.0000 ppm

Unionized Ammonia-Nitrogen – 0.1118 ppm
Ionized Ammonia-Nitrogen = 0.8882 ppm

14

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Carbon Dioxide

DESCRIPTION CODE

*Phenolphthalein Indicator, 1% *2246-E
*Carbon Dioxide Reagent B *4253DR-H

Direct Reading Titrator, 0-50 Range 0380
Test Tube, 5-10-12.9-15-20-25 mL, glass, w/cap 0608

*WARNING: Reagents marked with an * are considered to be potential health hazards.
For futher information see page 4.

The Direct Reading Titrator is calibrated in terms of carbon dioxide expressed as parts
per million (ppm) free CO2. Each minor division on the Titrator scale equals 1.0 ppm CO2.

12

1

NOTE:
results, test
a freshly obtained
sample, and avoid
splashing or prolonged
contact with air.

3

3

Fill Direct
Reading Titrator
(0380) with
Carbon Dioxide
Reagent B
(4253DR).

For best

Note:

Fill the test tube
(0608) to the
20 mL line
with the
sample water.

4

4

Insert the Titrator
into the center
hole of the test
tube cap.

water

sample

2

Add 2 drops of
Phenolphthalein
Indicator, 1%
(2246). If sample
turns red, no free
carbon dioxide is
present. If sample
is colorless,
proceed to Step 3.

5

5

While gently
swirling the
tube, slowly
press the
plunger to
titrate until a
faint pink color
develops and
persists for 30
seconds.

6

6

Read the test
result directly
from the
where the large
ring
Titrator meets the
Titrator barrel.
Record as Carbon
Dioxide.

scale

on the

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Result:

40 ppm

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NOTE: The Titrator is calibrated
in terms of carbon dioxide
expressed as
minor division on the Titrator scale
equals 1.0 ppm CO2.

ppm Free CO2. Each

15

Dissolved Oxygen

DESCRIPTION CODE

*Manganous Sulfate Solution *4167-G
*Alkaline Potassium Iodide Azide Reagent *7166-G
*Sulfuric Acid, 1:1 *6141WT -G

Sodium Thiosulfate, 0.025N 4169-H
Starch Indicator Solution 4170WT-G
Direct Reading Titrator, 0-10 Range 0377
Test Tube, 5-10-12.9-15-20-25 mL, glass, w/cap 0608
Pipet, plain, plastic, w/cap 0392
Water Sampling Bottle, 60 mL, glass 0688-DO

*WARNING: Reagents marked with an * are considered to be potential health hazards.
For futher information see page 4.

The Titrator is calibrated in terms of Dissolved Oxygen expressed as ppm Dissolved
Oxygen. Each minor division on the Titrator scale equals 0.2 ppm Dissolved Oxygen.

16

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DISSOLVED OXYGEN TEST PROCEDURE

Part 1 - Collecting the Water Sample

1

12

Rinse the Water
Sampling Bottle
(0688-DO) with
the sample water.

3

3

Remove the cap and allow
the bottle to fi ll.

56

5

2

Tightly cap the bottle, and
submerge it to the desired depth.

4

4

Tap the sides of the bottle to
dislodge any air bubbles.

6

Replace the cap while the
bottle is still submerged.

Retrieve the bottle
and make sure that
no air bubbles are
trapped inside.

17

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Part 2 - Adding the Reagents

NOTE: Be careful not to introduce air into the sample while adding
the reagents.

12

1

Remove the
cap from the
bottle.

3

3

5

Cap the bottle and mix by
inverting several times.
A precipitate will form.

56

Add 8 drops of
*Sulfuric Acid, 1:1
(6141WT).

2

Immediately add 8
drops of *Manganous
Sulfate Solution (4167)
and add 8 drops of
*Alkaline Potassium
Iodide Azide (7166).

4

4

Allow the precipitate
to settle below the
shoulder of the bottle.

6

Cap and gently invert the bottle
to mix the contents until the
precipitate has totally dissolved.
The solution
will be clear
yellow to
orange if the
sample contains
dissolved oxygen.

NOTE: At this point the sample has been “fi xed” and contact between the
sample and the atmosphere will not affect the test result. Samples may be
held at this point and titrated later.

18

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Part 3 - The Titration

12

12

0

Fill the titration tube
(0608) to the 20 mL line
with the fi xed sample.
Cap the tube.

0

0.1

0.1

0.2

0.2

0.3

0.3

0.4

0.4

0.5

0.5

0.6

0.6

0.7

0.7

0.8

0.8

0.9

0.9

1.0

1.0

Depress plunger of
the Titrator (0377).

3

3

Insert the Titrator into
the plug in the top of the
*Sodium Thiosulfate,

0.025N (4169) titrating
solution.

0

.

1

0

0

.

2

.

3

0

0

.

4

5

0

.

0

.

6

.

7

0

8

0

.

.

9

0

0

1

.

4

4

Invert the bottle and
slowly withdraw the
plunger until the large
ring on the plunger is
opposite the zero (0)
line on the scale.

NOTE: If small air bubbles appear in the titrator barrel, expel them
by partially fi lling the barrel and pumping the titration solution back
into the reagent container. Repeat until bubble disappears.

5

5

Turn the bottle
upright and remove
the Titrator.

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

NOTE: If the sample is
a very pale yellow, go to
Step 9.

1.0

0.9

0.8

0.7

0.6

0.5

0.4

0.3

0.2

0.1

0

continued . . .

19

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6

67

Insert the tip of the Titrator
into the opening of the
titration tube cap.

7

Slowly depress the
plunger to dispense the
titrating solution until
the yellow-brown color
changes to a very pale
yellow. Gently swirl the
tube during the titration
to mix the contents.

8

89

Carefully remove the
Titrator and cap. Do
not disturb the Titrator
plunger.

10

Cap the titration tube.
Insert the tip of the
Titrator into the
opening of the
titration tube cap.

12

12

Read the test result directly from the scale
where the large ring on the Titrator meets
the Titrator barrel. Record as ppm Dissolved
Oxygen. Each minor division on the Titrator
scale equals 0.2 ppm.

9

Add 8 drops of Starch
Indicator Solution
(4170WT). The sample
should turn blue.

11

Continue titrating until the blue

1110

color disappears and the solution
becomes colorless.

NOTE: If the plunger ring
reaches the bottom line on
the scale (10 ppm) before the

endpoint color change occurs,

refi ll the Titrator and continue
the titration. Include the value
of the original
reagent dispensed

when recording the test result.

amount of

(10 ppm)

NOTE: When testing is complete, discard the
titrating solution in the Titrator. Rinse Titrator
and titration tube thoroughly. DO NOT remove
plunger or adapter tip.

20

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Nitrate Nitrogen

DESCRIPTION CODE

*Mixed Acid Reagent *V -6278-J
*Nitrate Reducing Reagent *V-6279-C
Spoon, 0.1 g, plastic 0699
Test Tube, 2.5-5-10 mL, plastic, w/cap 0106
Dispenser Cap 0692
Octa-Slide 2 Viewer 1101
Nitrate Nitrogen Octa-Slide 2 Bar, 0.25-10.0 ppm 3109 -01

*WARNING: Reagents marked with an * are considered to be potential health hazards.
For futher information see page 4.

NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap
for refi ll reagents.

123

4

7

Insert Nitrate­Nitrogen Octa­Slide 2 Bar
(3109-01) into
Octa-Slide 2
Viewer (1101).

Wait 2 minutes.

2

MINUTES

Wait 10 minutes.

10

MINUTES

5

8

Fill a test tube
(0106) to the

2.5 mL line
with the water
sample.

Use the 0.1g
spoon (0699)
to add 0.1g
of *Nitrate
Reducing
Reagent
(V-6279).

Cap and
invert one
time before
inserting test
tube into
Octa-Slide 2
Viewer (1101).

6

9

Attach dispenser
cap (0692) to
the *Mixed Acid
Reagent (V-6278).
Fill tube to the
5mL

line.

Cap and mix.

Cap and invert
50-60 times for
one minute.

Insert test tube
into Octa-Slide 2
Viewer (1101).

10

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Match sample color
ppm Nitrate Nitrogen (NO

Conversions: ppm Nitrate-N (NO

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to color standard. Record results as

-N).

3

-N)

x 4.4 = ppm Nitrate (NO3)

3

21

Nitrite Nitrogen

DESCRIPTION CODE

*Mixed Acid Reagent *V -6278-J
*Color Developing Reagent *V-6281-D
Spoon, 0.1 g, plastic 0699
Test Tube, plastic, w/cap 0106
Dispenser Cap 0692
Octa-Slide 2 Viewer 1101
Nitrite Nitrogen Octa-Slide 2 Bar, 0.05-0.8 ppm 3437-01

*WARNING: Reagents marked with an * are considered to be potential health hazards.
For futher information see page 4.

NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278-J). Save this
cap for refi ll reagents.

1

4

Insert Nitrite­Nitrogen
Octa-Slide 2
Bar (3437-01)
into

the
Octa-Slide 2
Viewer (1101).

Use the 0.1 g
spoon (0699)
to add 0.1 g
of

*Color
Developing
Reagent
(V-6281).

23

Fill a test tube
(0106) to the

5

2.5 mL line
with the water
sample.

Cap and mix
by inverting
for one minute.

6

Dilute to the
*Mixed Acid

Reagent
(V-6278).

5mL line with

Wait 5
minutes.

5

MINUTES

78

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Insert test tube
into Octa-Slide 2
Viewer (1101).

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Match sample color
to color standard.
Record results as
ppm Nitrite
Nitrogen.

9

To convert to
multiply results by 3.3.
Record as ppm

Nitrite-N (NO3-N)
ppm Nitrite (NO

Nitrite,

Nitrite.

x 3.3 =

)

2

pH

DESCRIPTION CODE

*Wide Range Indicator *2218-G
Test Tube, plastic, w/cap 0106
Octa-Slide 2 Viewer 1101
Wide Range pH Octa-Slide 2 Bar, 5.0-10.0 3483-01

*WARNING: Reagents marked with an * are considered to be potential health hazards.
For futher information see page 4.

123

4

Insert Wide

Range pH

Octa-Slide 2

Bar (3483-01)

into the Octa-

Slide 2 Viewer

(1101).

Cap and mix.

5

Fill a test tube
(0106) to the
10 mL line
with the water
sample.

Insert test tube
into Octa-Slide 2
Viewer (1101).

6

Add 8 drops
of *Wide Range
pH Indicator
(2218).

Match sample
color to color
standard.
Record as pH.

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Salinity

DESCRIPTION CODE

*Salinity Indicator Reagent A *7460-E
*Salinity Titration Reagent B *7461-H

Demineralizer Bottle 1151
Test Tube, 5-10-12.9-15-20-25 mL, glass, w/cap 0608
Direct Reading Titrator, 0-20 Range 0378
Direct Reading Titrator, 0-1.0 Range 0376

*WARNING: Reagents marked with an * are considered to be potential health hazards.
For futher information see page 4.

Deminerlizer Bottle (Code 1151)

The Demineralizer Bottle will be the source of demineralized water for the salinity
test. It is partially fi lled with an ion exchange resin containing an indicator to show
when the exchange resin has been exhausted. The indicator will change from dark
green to yellow amber at which point the resin should be replaced.

Instructions For Use

1. Remove cap and fi ll bottle with water to be demineralized.

2. Recap, make sure spout is closed, and shake vigorously for 30 seconds.

3. Open spout, invert bottle, and gently squeeze to dipense the demineralized water.

Care and Storage

1. Do not heat water over 100°F.

2. Keep resin covered with water at all times.

3. Store bottle fi lled with enough water to cover resin completely.

The Titrator is calibrated in terms of Salinity expressed as ppt Salinity. Each minor
division on the Titrator scale equals 0.4 ppt Salinity.

24

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SALINITY TEST PROCEDURE

12

Fill the test
tube (0608)
to the 10 mL
line with
Demineralized
Water (1151).

4

Add 3 drops
of *Salinity
Indicator
Reagent A
(7460) to test
tube.

7

Insert the Titrator
into the center
hole of the test
tube cap.

Fill the 1.0 mL
Direct Reading
Titrator (0376) to
the 0 mark with
sample water.
Wipe off excess
water.

5

Cap and
gently swirl
to mix.
Sample will
turn yellow.

89

While gently
swirling the tube,
press the plunger
to add Salinity
Titration Reagent
B until yellow
color changes
to pink-brown.

3

Dispense 0.5 mL
of sample water
into test tube by
depressing plunger
until tip is at 0.5
mark. Discard
remaining sample
in Titrator.

6

Fill Direct
Reading
Titrator (0378)
with *Salinity
Titration Reagent
B (7461).

Read the
test result
directly from the scale
where the large ring
on the Titrator meets
the Titrator barrel.
Record as ppt salinity.

Note: Each Titration Division = 0.4 ppt Salinity

25

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3635-05

0377

0378

0380

4170

PS-G

6141

WT-G

4169-H

7461-H

4253DR-H

3437-01

V-6279-C

7460-E

2246-E

1066

7166-G

1151

V-6281-C

0382

4167-G

0376

4493DR-H

V-6278-J

0608

-DO

0688

0608

2311-PG-E

V-6278-J

3441-01-SW

1101

3109-01

3483-01

0106

0106 01060106

01060106

3982

WT-G

2218-G

3979

WT-G

0688

3978

LWT-H

26

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