LaMotte 3561-01 User Manual

HYDROPONICS KIT

CODE 3561-01

QUANTITY CONTENTS CODE

120 mL *Mixed Acid Reagent *V-6278-J
5 g *Nitrate Reducing Reagent *V-6279-C
60 mL *VM Phosphate Reagent *4410-H
30 mL *Wide Range Indicator *2218-G
30 mL *Sodium Hydroxide, 1.0N *4004WT-G
60 mL *Hydrochloric Acid, 1.0N *6130WT-H
15 mL *Sodium Hydroxide Reagent w/Metal Inhibitors *4259-E
5 g *Tetraphenylboron Powder *6364-C
6 Test Tubes, plastic, w/caps 0106
1 Double Tube, potassium, w/cap 0780
1 Spoon, 0.1 g, plastic 0699
1 Spoon, 0.05 g, plastic 0696
1 Pipet, 1.0 mL 0354
1 T est Tube Brush 0514
1 Demineralizer Bottle 1152
1 Octa-Slide 2 Viewer 1101
1 pH Wide Range Octa-Slide 2 Bar, 4.5-8.0 3424-01
1 Phosphorus Octa-Slide 2 Bar, 3.0-30 ppm 5305-01
1 Nitrate Octa-Slide 2 Bar, 0.25-10.0 ppm 3109-01
1 Plant Nutrition Studies 1596
1 Graduated Cylinder, 25 mL 0417
1 Dispenser Cap 0692

*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents go to
www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

To order individual reagents or test kit components, use the specifi ed code number.
Read Demineralizer Bottle instruction manual before proceeding.
The Demineralizer Bottle (1152) will be the source of all deionized water used in

these tests.

USE OF THE OCTA-SLIDE 2 VIEWER

The Octa-Slide 2 Viewer should be held so
non-direct light enters through the back of
the Viewer. Insert the reacted sample into
the top of the Viewer. Slide the Octa-Slide 2
Bar into the Viewer and match the color of
the reaction to the color standards.

WARNING! This set contains chemicals

that may be harmful if misued. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision.

DILUTION PROCEDURE

If the test result in the nitrate, phosphate or potassium test is greater than the highest
value it is necessary to perform a dilution. Use the 1.0 mL pipet (0354) and the 25 mL
graduated cylinder (0417) as follows:

AMOUNT

OF SAMPLE

DILUTE TO MULTIPLY

RESULT BY

1.0 mL 5.0 mL 5

1.0 mL 10.0 mL 10

1.0 mL 20.0 mL 20

1.0 mL 25.0 mL 25

Use deionized water from the Demineralizer Bottle for all dilutions.

pH

A pH value of 7.0 indicates a neutral solution. Values above 7.0 indicate alkaline
solutions, while values below pH 7.0 indicate acid solutions.

1. Fill a test tube (0106) to 5.0 mL line with sample solution.

2. Add 4 drops of *Wide Range Indicator (2218). Cap and mix.

3. Insert pH Wide Range Octa-Slide 2 Bar (3424-01) into Octa-Slide 2 Viewer (1101).

4. Insert test tube into the Octa-Slide 2 Viewer. Match sample color to a color
standard.

NOTE: If the pH of the sample is not between 6.2 and 6.8, it must be adjusted
to 6.5 before proceeding the with the Nitrate, Phosphorus, and Potassium tests.
If the pH is below 6.2 add *Sodium Hydroxide, 1.0N (4004), one drop at a time,
periodically checking the pH until it is brought into the range of 6.2 to 6.8. If the
pH is above 6.8, add *Hydrochloric Acid, 1.0N (6130) in the same manner.

NITRATE NITROGEN

The range of the color standards in the Nitrate Nitrogen Color Bar is from 0.25 to 10
ppm Nitrate Nitrogen. If the nutrient solution is above this range, it should be diluted
to bring the readings within the range. The dilution will depend upon the amount of
nitrate in the solution. A 1 to 20 dilution is suggested.

NOTE: Place dispenser cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap
for refi ll reagents.

1. Fill a test tube (0106) to the 2.5 mL line with nutrient solution.

2. Add *Mixed Acid Reagent (V-6278) to the test tube to fi ll to the 5.0 mL line. Cap
and mix. Wait two minutes.

3. Use the 0.1 g spoon (0699) to add one level measure (avoid any excess) of *Nitrate
Reducing Reagent (V-6279). Cap and invert the tube 50-60 times in one minute.
Wait 10 minutes.

4. Insert Nitrate Octa-Slide 2 Bar (3109-01) into Octa-Slide 2 Viewer (1101).

5. Insert the tube into the Octa-Slide 2 Viewer . Match sample color to a color standard.
If the original sample was diluted, multiply the reading by 20 to obtain the fi nal
result in ppm Nitrate Nitrogen.

PHOSPHORUS

The range of the color standards in the phosphorus comparator is from 3 to 30 ppm
Phosphorus. Most nutrient solutions will have to be diluted to bring the reading within
this range. A 1 to 2 dilution is suggested and involves diluting 5 mL of solution to 10
mL with deionized water.

1. Fill a test tube (0106) to the 5.0 mL line with nutrient sample.

2. Use the 1.0 mL pipet (0354) to add 1.0 mL of *VM Phosphate Reagent (4410).
Cap and mix. Wait 5 minutes.

3. Insert Phosphorous Octa-Slide 2 Bar (5305-01) into Octa-Slide 2 Viewer (1101).

4. Insert test tube into the Octa-Slide 2 Viewer. Match sample color to a color
standard. If the original sample was diluted, multiply the reading by 2 to obtain
the fi nal result in ppm Phosphorus.

POTASSIUM

A turbidimetric method is used for the determination of potassium in the nutrient solution.
It is suggested that the nutrient solution be diluted to a 1 to 5 ratio with deionized
water to bring the sample concentration within the range of the test. A 1 to 5 dilution
involves diluting 2 mL of the sample to 10 mL with deionized water.

1. Remove the square inner tube and collar from the double tube (0780).

2. Fill the round tube to the fi rst line (8 mL) with nutrient sample.

3. Fill to the second line (16 mL total) with deionized water.

4. Add 2 drops of *Sodium Hydroxide Reagent (4259). Cap and mix.

5. Use the 0.5 g spoon (0696) to add one level measure of Tetraphenylboron Powder
(6364). Cap and shake vigorously until all the powder has dissolved. A white
precipitate will form immediately. Wait 5 minutes.

6. Shake the tube again. Remove cap and slowly insert the square tube with the collar.
The square tube will slide up and down through the collar and fi ll with liquid.

7. Viewing from above, adjust the square tube into the turbid solution until the black
dot on its base is obscured. Hold the round tube at the top to avoid blocking light.

Record the height of the liquid in the square tube on the scale. Calibrations
represent parts per million potassium. If the original sample has been diluted 1
to 5, the reading should be multiplied by 5 to obtain the potassium content of the
original sample.

NOTE: Brush tubes thoroughly after each use.

LaMOTTE COMPANY

Helping People Solve Analytical Challenges

PO Box 329 • Chestertown • Maryland • 21620 • USA

800-344-3100 • 410-778-3100 (Outside USA) • Fax [phone]

Visit us on the web at www.lamotte.com

63561-01 2/14

®