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AMMONIA NITROGEN TEST KIT
OCTA-SLIDE METHOD
CODE 3351-02
QUANTITY CONTENTS CODE
30 mL Ammonia Nitrogen Reagent #1 4797WT-G
30 mL *Ammonia Nitrogen Reagent #2 *4798WT-G
2 Test Tubes, 2.5-5.0-10.0 mL, plastic, w/caps 0106
1 Ammonia Nitrogen Octa-Slide 2 Bar,0.2-3.0 ppm 3438-01
1 Octa-Slide 2 Viewer 1101
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents go to
www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specifi ed code number.
USE OF THE OCTA-SLIDE 2 VIEWER
The Octa-Slide 2 Viewer should be held so
non-direct light enters through the back of
the Viewer. Slide the Octa-Slide 2 Bar into
the Viewer. Insert the reacted sample into
the top of the Viewer. Match the color of the
reaction to the color standards.
WARNING! This set contains chemicals
that may be harmful if misused. Read
cautions on individual containers
carefully. Not to be used by children
except under adult supervision
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PROCEDURE
1. Insert the Ammonia Nitrogen Octa-Slide (3438-01) into the Octa-Slide Viewer
(1101).
2. Fill test tube (0106) to the 5 mL line with sample water.
3. Add 4 drops of Ammonia Nitrogen Reagent #1 (4797WT). Cap and mix. Wait 1
minute.
NOTE: When testing salt (sea) water, increase the amount of Ammonia Nitrogen
Reagent #1 to 8 drops.
4. Add 12 drops of *Ammonia Nitrogen Reagent #2 (4798WT). Cap and mix. Wait
fi ve minutes.
NOTE: When testing salt water, the reading should be taken after 1 minute to
prevent precipitation.
5. Insert test tube into the top of the viewer. Match sample color to a color standard.
Record as ppm Ammonia Nitrogen (NH
CONVERSIONS
-N).
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To express results as Ammonia (NH3):
ppm Ammonia (NH3) =
ppm Ammonia Nitrogen (NH3-N) x 1.2
To express results as Ammonium (NH
ppm Ammonium (NH
+
):
4
+
) =
4
ppm Ammonia Nitrogen (NH3–N) x 1.3
Ammonia in water occurs in two forms: toxic unionized ammonia (NH3) and
the relatively non-toxic ionized form, ammonium ion (NH
+
). This test method
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measures both forms as ammonia nitrogen (NH3–N) to give the total ammonianitrogen concentration in water. The actual proportion of each compound depends on
temperature, salinity, and pH. A greater concentration of unionized ammonia is present
when the pH value and salinity increase.
1. Consult the table to fi nd the percentage that corresponds to the temperature, pH,
and salinity of the sample.
2. To express the test result as ppm Unionized Ammonia Nitrogen (NH3–N),
multiply the total ammonia nitrogen test result by the percentage from the table.
3. To express the test result as ppm Ionized Ammonia Nitrogen (NH
+
–N), subtract
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the unionized ammonia-nitrogen determined in step 2 from the total ammonia
nitrogen.
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pH FW
1
10°C
SW
15°C 20°C
2
FW SW FW SW FW SW
25°C
7.0 0.19 — 0.27 — 0.40 — 0.55 —
7.1 0.23 — 0.34 — 0.50 — 0.70 —
7.2 0.29 — 0.43 — 0.63 — 0.88 —
7.3 0.37 — 0.54 — 0.79 — 1.10 —
7.4 0.47 — 0.68 — 0.99 — 1.38 —
7.5 0.59 0.459 0.85 0.665 1.24 0.963 1.73 1.39
7.6 0.74 0.577 1.07 0.836 1.56 1.21 2.17 1.75
7.7 0.92 0.726 1.35 1.05 1.96 1.52 2.72 2.19
7.8 1.16 0.912 1.69 1.32 2.45 1.90 3.39 2.74
7.9 1.46 1.15 2.12 1.66 3.06 2.39 4.24 3.43
8.0 1.83 1.44 2.65 2.07 3.83 2.98 5.28 4.28
8.1 2.29 1.80 3.32 2.60 4.77 3.73 6.55 5.32
8.2 2.86 2.26 4.14 3.25 5.94 4.65 8.11 6.61
8.3 3.58 2.83 5.16 4.06 7.36 5.78 10.00 8.18
8.4 4.46 3.54 6.41 5.05 9.09 7.17 12.27 10.10
8.5 5.55 4.41 7.98 6.28 11.18 8.87 14.97 12.40
1
Freshwater data from Trussel (1972).
2
Seawater values from Bower & Bidwell (1978). Salinity for Seawater values = 34%
at an ionic strength of 0.701 m.
FOR EXAMPLE:
A fresh water sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as total
Ammonia-Nitrogen.
1. The percentage from the table is 11.18% (or 0.1118).
2. 1 ppm total Ammonia Nitrogen x 0.1118 = 0.1118 ppm Unionized
Ammonia-Nitrogen
3. Total Ammonia-Nitrogen 1.0000 ppm
Unionized Ammonia-Nitrogen - 0.1118 ppm
Ionized Ammonia-Nitrogen = 0.8882 ppm
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