*WARNING: Reagents marked with an * are considered to be potential health
hazards.
reagent label, in the contents
that follows or precedes the four digit code number. For example if code is
4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
To view or print a Safety Data Sheet (SDS) for these reagents go to
Search the four digit reagent code number listed on the
list or in the test procedures. Omit any letter
3240-LI-MN-CL
3240-LI-QG-CL
To order individual reagents or test kit components, use the specied code
Drinking water supplies and distribution systems, swimming pool and
spas, sewage and chlorinated waste waters, process waters and sanitizing
solutions.
RANGE
0 to 4.0 ppm Chlorine (may be extended by dilution)
0 to 7.0 ppm Bromine (may be extended by dilution)
METHOD
In the absence of Iodide, Free Available Chlorine reacts instantly with the
buered diethyl-p-phenylenediamine indicator (DPD) to produce a pink-red
color in proportion to the amount of chlorine present. Subsequent addition of
potassium iodide produces a rapid color response from the combined forms
of chlorine (chloramines). In buered samples, bromine reacts with disthyl-
p-phenylene diamine (DPD) to produce a pink-red color in proportion to the
concentration of bromine present.
HANDLING & PRESERVATION
Chlorine and bromine in aqueous solutions, particularly weak solutions, are
not stable. Exposure to sunlight or agitation will accelerate the reduction of
chlorine and bromine. For chlorine samples, ll sample containers to the top
and cap tightly. Analyze samples as soon as possible after collection. Samples
to be analyzed for bromine cannot be preserved or stored.
INTERFERENCES
The only interfering substance likely to be encountered is oxidized
manganese. The extent of this interference can be determined by treating a
sample with sodium arsenite to destroy the chlorine or bromine present, so
that the amount of interference can be estimated. Chlorine, bromine, and
iodine will give a positive result and should be considered an interference
unless the test is being performed specically for that parameter.
CALIBRATION
The colorimeter is precalibrated. In order to comply with NPDWR or NPDES
reporting regulations, the calibration should be checked periodically by using
a set of reference standards including a 0 mg/L blank and 0.3, 1.0, and 3.5
mg/L chlorine. To prepare these standards, a LaMotte 1000 mg/L standard
chlorine equivalent solution (Code 3858) is available. Consult with your local
regulatory agency to determine standardization frequency.
6. Insert tube into chamber, close
lid and select Scan Blank.
Chlorine
7. Remove tube from colorimeter.
Add 5 drops of DPD 1A Free
Chlorine Reagent (P-6740) and
add 5 drops of *DPD 1B Free
Chlorine Reagent (P-6741), Cap
and invert to mix. The solution
will be pink if chlorine is present.
Read within 30 seconds.
8. Immediately insert tube into
chamber. Close lid. Select Scan Sample. Record result as Free
Chlorine.
0.99 ppm
Scan BLank
Scan Sample
12:00:00 001/500
PROCEDURE – COMBINED CHLORINE
9. Remove tube from colorimeter.
Add 5 drops of *DPD 3 Total
Chlorine Reagent (P-6743). Cap
and invert to mix. An increase
in color represents Combined
Chlorine.
DPD
DPD
024 Chlorine L DPD
1A
DPD
3
1B
NOTE: For wastewater samples, Standard Methods for the Examination of Water and
Wastewater recommends waiting 2 minutes for full color development when testing
10. Insert tube into chamber, close
lid and select Scan Sample.
Record result as Total Chlorine
(Monochloramine, Dichloramine,
and Nitrogen Trichloride)
present in the water sample.
11. Subtract free chlorine reading from total chlorine reading to obtain
concentration of combined chlorine.
Chlorine
12. Press to turn o the colorimeter or press
EXIT
to exit to a
previous menu or make another menu selection.
Levels of chlorine about 4 mg/L will cause a bleaching eect on the DPD
indicator, and may give a false indication that no chlorine is present. If if is
possible that the chlorine concentration is greater than 4 mg/L, perform test
on a diluted sample and multiply the result by appropriate dilution factor.
CAUTION: DO NOT leave reacted samples in test tubes. Discard sample and
thoroughly rinse tubes. If allowed to remain, DPD wil stain tubes, signigicantly
impairing the operation of the colorimeter. If necessary, acid wash, and
vigorously clean glassware with test tube brush and detergent.
NOTE: For the most accurate results, samples over 4 ppm chlorine should be
diluted with chlorine demand free water and re-tested.
NOTE: The meter will remember the last scanned blank reading. It is not
necessary to scan a blank each time the test is performed. To use the
previous blank reading, instead of scanning a new one, scroll to Scan Sample
and proceed. For the most accurate results, the meter should be blanked
before each test and the same tube should be used for the blank and the
reacted sample.
6. Insert tube into chamber, close
lid and select Scan Blank.
Bromine
7. Remove tube from colorimeter.
Add 5 drops of DPD 1A Free
Chlorine Reagent (P-6740) and
add 5 drops of *DPD 1B Free
Chlorine Reagent (P-6741), Cap
and invert to mix. The solution
will be pink if bromine is present.
Read within 30 seconds.
8. Immediately insert tube into
chamber. Close lid. Select
Scan Sample. Record result as
Bromine.
NOTE: For the most accurate results, samples over 7 ppm bromine should be
diluted with chlorine demand free water and re-tested.
NOTE: The meter will remember the last scanned blank reading. It is not
necessary to scan a blank each time the test is performed. To use the
previous blank reading, instead of scanning a new one, scroll to Scan Sample
and proceed. For the most accurate results, the meter should be blanked
before each test and the same tube should be used for the blank and the
reacted sample.
The meter should be calibrated with free chlorine standards. The calibration
should be done with a distilled or deionized water blank and one chlorine
standard of known concentration. The concentration of the calibration
Calibration
standard should be similar to the expected concentration of the sample that
will be tested.
To perform a bromine calibration, prepare a bromine with a concentration
that is equivenlent to a chlorine dioxide standard that is within the range of
the chlorine dioxide test. (1 ppm chlorine = 1.9 ppm bromine). Follow the
procedure below but select the bromine test.
Chlorine Secondary Standards (Code 4140-03) are available to verify the
performance of the meter.
CHLORINE CALIBRATION PROCEDURE
1. Press and briey hold to
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
will appear.
4. Rinse a clean tube (0290)
three times with the chlorine
standard. Fill to the 10 mL line
with the chlorine standard. Cap
the tube. Dry the tube with a
lint-free cloth.
5. Insert tube into chamber, close
lid and select Scan Blank.
Calibration
6. Remove tube from colorimeter.
Add 5 drops of DPD 1A Free
Chlorine Reagent (P-6740) and
add 5 drops of *DPD 1B Free
Chlorine Reagent (P-6741), Cap
and invert to mix. The solution
will be pink if chlorine is present.
Read within 30 seconds.
7. Immediately insert tube into
chamber. Close lid. Select Scan Sample.
9. Press
A reverse font (light background
with dark characters) will appear
to indicate that the reading can
be adjusted.
Calibration
ENTER
to select Calibrate.
024 Chlorine L DPD
0.99 ppm
∧, ∨=Edit, ENTER=Save
∧ +ENTER=Default
12:00:00 001/500
10. Press or to
adjust the value shown to the
concentration of the prepared
standard, 1.00 in this example.
NOTE: A maximum adjustment
of 25% is possible.
11. Press
ENTER
to save the value.
To leave the Calibration
procedure without saving the
adjustment, press
Press
time to return to the default
value.
The calibration has now been
standardized and can be used
for testing. Scroll to Scan Blank
and begin testing.
The default setting for the data logger is enabled. The meter will log the last
500 data points. The counter in the center bottom of the display will show how
many data points have been logged. The display will show 500+ when the data
logger has exceeded 500 points and the data points are being overwritten.
1. From the Editing Menu, press
or to scroll to
Logging.
2. Press
ENTER
to select Logging.
3. Press or to scroll to
desired function.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
Logging
Display Test Log
Logging Enabled
Logging Disabled
Erase Log
12:00:00 001/500
Logging
Display Test Log
Logging Enabled
Logging Disabled
Erase Log
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about 1
second and return to the EditingMenu.
The backlight illuminates the display for enhanced viewing. The default setting
is 10 seconds. If Button Control is chosen the backlight button on the key pad
will act as an on/o switch and the backlight will remain on or o when the
meter is being used. When one of the other settings – 10, 20 or 30 seconds –
is chosen, the display will be illuminated for the specied amount of time after
any button is pressed.
NOTE: The backlight feature uses a signicant amount of power. The longer
the backlight is on, the more frequently the battery will have to be charged if
the USB/Wall Adapter is not being used.
1. From the Editing Menu, press
or to scroll to
Backlight Time.
2. Press
ENTER
to select Set
Backlight Time.
3. Press or to scroll to
desired option.
Editing Menu
Logging
Factory Setup
Set Power Save
Set Backlight Time
12:00:00 001/500
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about 1
second and the meter will return
to the Editing Menu.
There are seven languages available: English, Spanish, French,
Portuguese, Italian, Chinese, and Japanese.
1. From the Editing Menu, press
or to scroll to
Select Language.
2. Press
ENTER
to select Select
Language.
3. Press or to scroll to
desired language.
Editing Menu
Factory Setup
Set Power Save
Set Backlight Time
Select Language
12:00:00 001/500
Select Language
English
Spanish
French
Portugese
12:00:00 001/500
Select Language
English
Spanish
French
Portugese
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about 1
second and the meter will return
to the Editing Menu.
Set Power Save
Set Backlight Time
Editing Menu
Bluetooth Menu
Select Language
12:00:00 001/500
NOTE: If meter unintentionally switches to another language, use the
procedure above to reset the meter to the desired language. For example, to
reset the meter to English:
Long menus, such as All Tests, incorporate a looping feature which allows
the user to quickly reach the last choice in the menu from the rst choice.
In a looping menu the last choices in the menu are above the rst choice
and scrolling upward moves through the menu in reverse order. Scrolling
downward moves through the menu from rst choice to last but the menu
starts over following the last choice. So all menu choices can be reached by
scrolling in either direction. The diagrams below demonstrate a looping menu.
The meter may be interfaced with any Windows-based 64 bit computer
by using the LaMotte WaterLink Connect program and a USB cable. The
program will store test information and results in a local database, and allow
for exporting this data to a comma separated value (CSV) le. The meter
will send the following data: time/date stamp, name of test, sample value,
sample units, meter name, and location. To download WaterLink go t
BATTERY
BATTERY/AC OPERATION
The colorimeter may be operated on battery power using a USB wall adapter
or USB computer connection. If using the meter as a bench top unit, use the
wall adapter if possible to extend the battery life. The meter will remain on
when the USB adapter is used.
To charge the battery with the wall adapter, plug the smaller end of the USB
cable (USB mini B connector) into the meter and the larger end of the USB
cable (USB type A connector) into the wall adapter. Plug the wall adapter into
an AC outlet. Reinsert the rubber USB port plug after charging. To charge the
battery from a computer, plug the smaller end of the USB cable (USB mini B
connector) into the meter and the larger end of the USB cable (USB Type A
connector) into a USB port on a computer. The battery icon will show no bars
and ash when the unit rst turns on. Then the indicator will indicate the
battery status by showing 0, 1, 2, 3, or 4 bars.
It will take 6 hours to fully charge a low battery. The battery icon will ash
when the battery is charging. The battery icon will show four bars and stop
ashing when it is fully charged. The charging circuit will automatically switch
to a oat charge when the battery is fully charged. The charger may remain
connected. Some computers will NOT supply power to their USB ports during
standby operation. The wall adapter will charge the unit continuously.
Storing the meter above ambient room temperature will decrease the battery
charge more quickly than storage at room temperature. If the meter does
not turn on, it means that the battery is at a very low charge. Charging the
battery with the wall adapter in this state may take up to 10 hours. At low
temperatures, approaching 0 °C, the battery will charge more slowly. It will not
charge at all below 0 °C. The battery icon will show no bars and continuously
ash if the battery is getting low but the unit will still operate normally. A
“Low Battery” message on the status bar of the display will replace the time
when the battery voltage is too low for proper operation and accuracy may
be degraded. A “Shutdown Low Batt” message on the display will appear for
a few seconds before the power is switched o when the battery is too low to
operate the unit. When the battery icon simultaneously ashes bars 1 and 2
followed by bars 3 and 4, it is an indication that the battery is damaged and
technical support should be contacted.
To extend the battery life:
• Shut down the unit with the power switch when not taking
measurements or use the power save option to have the unit
automatically turn o after 5 minutes.
• Store the unit at a moderate temperature.
• Fully charge the battery before storing the unit for extended periods
of time.
• Fully charge the battery at least once per year. Failure to do so may
result in a permanently drained battery.
• Limit backlight use. The unit consumes 3X normal power with the
backlight on. Set the backlight time option to 10 seconds, or select
“Button Control” and keep the backlight o.
BATTERY REPLACEMENT
The lithium ion battery used in this unit should last for many years with
normal use. When it no longer powers the unit long enough to meet testing
requirements it will need to be replaced. Lithium ion batteries that are
properly charged and stored do not usually lose all capacity; they just have
less capacity after hundreds of charge cycles. This unit uses a custom battery
assembly that is only available from LaMotte Company. Battery replacement
must be performed at a LaMotte authorized repair facility. The water resistant
housing of this meter should not be opened by the user
MAINTENANCE
CLEANING
Clean the exterior housing with a damp, lint-free cloth. Do not allow water
to enter the light chamber or any other parts of the meter. To clean the
light chamber and optics area, point a can of compressed air into the light
chamber and blow the pressurized air into the light chamber. Use a cotton
swab dampened with Windex® window cleaner to gently swab the interior of
the chamber. Do not use alcohol; it will leave a thin residue over the optics
when dry.
Waste Electrical and Electronic Equipment (WEEE)
Natural resources were used in the production of this equipment. This
equipment may contain materials that are hazardous to health and the
environment. To avoid harm to the environment and natural resources, the
use of appropriate take-back systems is recommended. The crossed out
wheeled bin symbol on the meter encourages the use of these systems when
disposing of this equipment.
Take-back systems will allow the materials to be reused or recycled in a
way that will not harm the environment. For more information on approved
collection, reuse, and recycling systems contact local or regional waste
administration or recycling services.
The meter is a portable, microprocessor controlled, direct reading colorimeter.
It has a graphical liquid crystal display and 6 button keypad. These allow the
user to select options from the menu driven software, to directly read test
results or to review stored results of previous tests in the data logger. The
menus can be displayed in seven dierent languages.
The LaMotte tests are precalibrated for LaMotte reagent systems.
The colorimeter displays the result of these tests directly in units of
concentration.
The optics feature a colored LED. The LED has a corresponding silicon
photoiode with an integrated interference lter. The interference lter selects
a narrow band of light from the corresponding LED for the colorimetric
measurements.
A USB wall adapter, USB computer connection or lithium battery powers the
colorimeter.
GENERAL OPERATING INFORMATION
The operation of the colorimeter is controlled by the menu driven software
and user interface. A menu is a list of choices. This allows a selection of
various tasks for the colorieter to perform, such as scan blank and scan
sample. The keypad is used to make menu selections that are viewed on the
display.
THE KEYPAD
The keypad has 6 buttons which are used to perform specic tasks.
ENTER
EXIT
This button will scroll up through a list of menu selections.
The button is used to select choices in a menu viewed on the
display.
This button controls the backlight on the display.
This button will scroll down through a list of menu selections.
The display allows menu selections to be viewed and selected. These
selections instruct the colorimeter to perform specic tasks. The menus are
viewed in the display using two general formats that are followed from one
menu to the next. Each menu is a list of choices or selections.
The display has a header line at the top and a footer line at the bottom. The
header displays the title of the current menu. The footer line displays the
time and the date, the data logger status and the battery status. The menu
selection window is in the middle of the display between the header and the
footer.
The menu selection window displays information in two general formats. In
the rst format only menu selections are displayed. Up to 4 lines of menu
selections may be displayed. If more selections are available they can be
viewed by pressing the arrow buttons to scroll the other menu
selections into the menu selection window. Think of the menu selections as
a vertical list in the display that moves up or down each time an arrow button
is pressed. Some menus in the colorimeter are looping menus.
The top and bottom menu choices are connected in a loop. Scrolling down
past the bottom of the menu will lead to the top of the menu. Scrolling up
past the top of the menu will lead to the bottom of the menu.
HeaderMenu Title
Main Window Selection First Choice
Second Choice
Third Choice
Another
Footer
12:00:00 001/500 P
And Another
And So On
A light bar will indicate the menu choice. As the menu is scrolled through, the
light bar will highlight dierent menu choices. Pressing the
ENTER
button will
select the menu choice that is indicated by the light bar.
In the second format the menu choice window takes advantage of the
graphical capabilities of the display. Large format graphic information, such
as test results or error messages or the LaMotte logo is displayed. The top
two lines of the display are used to display information in a large, easy to read
format. The menus work in the same way as previously described but two lines
of the menu are visible at the bottom of the display.
HeaderMenu Title
Message or Result Window
Result or
Message
Another
Main Window Selection And Another
Footer
As described previously, the
current menu and a return to the previous menu. This allows a rapid exit from
an inner menu to the main menu by repeatedly pushing the
Pushing at any time will turn the colorimeter o.
The display may show the following messages:
Battery Status
More choices are available and can be viewed by scrolling
up and/or down through the display.
EXIT
12:00:00 001/500
And So On
Last Choice
button allows an exit or escape from the
EXIT
button.
HeaderIdenties the current menu and information on units and
reagent systems if applicable.
FooterIn the data logging mode the number of the data point
is displayed and the total number of data points in the
memory will be shown. The footer also shows current time
and battery status
The colorimeter uses one type of tube (Code 0290) for all test factors.
The handling of the tubes is of utmost importance. Tubes must be clean and
free from lint, ngerprints, dried spills and signicant scratches, especially
the central zone between the bottom and the sample line.
Scratches, ngerprints and water droplets on the tube can cause stray light
interference leading to inaccurate results. Tubes that have been scratched in
the light zone through excessive use should be discarded and replaced with
new ones.
Tubes should always be washed on the inside and outside with mild detergent
prior to use to remove dirt or ngerprints. The tubes should be allowed to air-
dry in an inverted position to prevent dust from entering the tubes. Dry tubes
should be stored with the caps on to prevent contamination.
After a tube has been lled and capped, it should be held by the cap and the
outside surface should be wiped with a clean, lint-free absorbent cloth until it
is dry and smudge-free. Handling the tube only by the cap will avoid problems
from ngerprints. Always set the clean tube aside on a clean surface that will
not contaminate the tube. It is imperative that the tubes and light chamber be
clean and dry. The outside of the tubes should be dried with a clean, lint-free
cloth or disposable wipe before they are placed in the meter chamber.
Tubes should be emptied and cleaned as soon as possible after reading a
sample to prevent deposition of particulates on the inside of the tubes.
Variability in the geometry of the glassware and technique is the predominate
cause of variability in results. Slight variations in wall thickness and the
diameter of the tubes may lead to slight variations in the test results. To
eliminate this error the tubes should be placed in the chamber with the same
orientation each time.
Chambers which have been scratched through excessive use should be
discarded and replaced with a new one.
SAMPLE DILUTION TECHNIQUES
If a test result is out of the range of the meter, it must be diluted. The test
should then be repeated on the diluted sample. The following table gives quick
reference guidelines for dilutions of various proportions.
Amount of SampleDeionized Water to Bring
Final Volume to 10 mL
10 mL0 mL1
5 mL5 mL2
2.5 mL7.5 mL4
1 mL9 mL10
0.5 mL9.5 mL20
All dilutions are based on a nal volume of 10 mL, so several dilutions will
require small volumes of the water sample. Graduated pipets should be
used for all dilutions. If volumetric glassware is not available, dilutions can
be made with the colorimeter tube. Fill the tube to the 10 mL line with the
sample and then transfer it to another container. Add 10 mL volumes of
deionized water to the container and mix. Transfer 10 mL of the diluted
sample to the colorimeter tube and follow the test procedure. Repeat the
dilution and testing procedures until the result falls within the range of the
calibration. Multiply the test result by the dilution factor. For example, if 10 mL
of the sample water is diluted with three 10 mL volumes of deionized water,
the dilution factor is four. The test result of the diluted sample should be
multiplied by four.
GENERAL INFORMATION
PACKAGING AND DELIVERY
Experienced packaging personnel at LaMotte Company assure adequate
protection against normal hazards encountered in transportation of
shipments.
After the product leaves LaMotte Company, all responsibility for safe delivery
is assured by the transportation company. Damage claims must be led
immediately with the transportation company to receive compensation for
damaged goods.
GENERAL PRECAUTIONS
READ THE INSTRUCTION MANUAL BEFORE ATTEMPTING TO SET UP OR
OPERATE THE METER. Failure to do so could result in personal injury or
damage to the meter. The meter should not be used or stored in a wet or
corrosive environment. Care should be taken to prevent water from wet tubes
from entering the meter chamber.
NEVER PUT WET TUBES IN THE METER.
SAFETY PRECAUTIONS
Read the labels on all LaMotte reagent containers prior to use. Some
containers include precautionary notices and rst aid information. Certain
reagents are considered hazardous substances and are designated with
a * in the instruction manual. Safety Data Sheets (SDS) can be found at
Keep equipment and reagent chemicals out of the reach of young children.
Ensure that the protection provided by this equipment is not impaired. Do not
intall or use this equipment in a manner that is not indicated in this manual.
The colorimeter has been independently tested and has earned the European
CE Mark of compliance for electromagnetic compatibility and safety. To view
certicates of compliance, go t
IP67 Certication
The meets IP67 standards for protection against dust and immersion only
when the USB port plug is in place. Documentation is available at www.
TROUBLESHOOTING
ERROR MESSAGES
Over Range
If the message OVERRANGE is displayed when scanning a sample, the
sample may be over range or under range. If the sample is over range the
sample should be diluted and tested again (see Sample Dilution Techniques
and Volumetric Measurements, page 27).
If overrange is displayed, press
ENTER
to continue testing on diluted
samples.
Note: After pressing
overrange cncentration will be
displayed. This concentration is an
approximation only.
As with all pre-calibrated meters, it is highly recommended, even if not
required by regulations, that the user periodically verify the performance of
the meter by running standards with a predetermined concentration. Results
outside of specication are an indication that the meter needs to be adjusted.
This can be done following the user calibration described on page 24. If the
user calibration fails to properly adjust the meter then the meter should be
returned to LaMotte Company for recalibration. (See page 30).
STRAY LIGHT
The colorimeter should have no problems with stray light. Make sure that the
sample compartment lid is always fully closed.
TROUBLESHOOTING GUIDE
PROBLEMREASONSOLUTION
Flashing
“Low Battery”Battery voltage is very
“Shut Down Low Batt”
Shut Down
“Overrange”Sample is outside of
Low battery. Readings
are reliable.
low. Readings are not
reliable.
Battery is too low to
operate the unit.
acceptable range.
Charge battery or use
USB wall/computer
adapter.
Charge battery or use
USB wall/computer
adapter.
Charge battery or use
USB wall/computer
adapter.
Dilute sample and
test again.
Unusually large
negative or positive
readings when
performing calibration
Incorrect standards
used to calibrate meter.
Use fresh 0.0
standard in clean
tube. Reset meter
to factory default
settings. Recalibrate
meter.