LaMotte 2020wi User Manual

2020
we/wi
1970-EPA 1970-ISO
CONTENTS
GENERAL INFORMATION
 Packaging and Delivery 5  General Precautions 5  Safety Precautions 5  Limits of Liability 6  Specifi cations 6  Statistical & Technical Defi nitions 7  Contents and Accessories 9  EPA Compliance 10  ISO Compliance 10  CE Compliance 10  IP67 Compliance 10  Warranty 10  Register Your Meter 10
COMPUTER CONNECTION 11 BATTERY/AC OPERATION 11 TURBIDITY
 What is Turbidity? 13  How is Turbidity Measured? 13  Turbidity Units 15  Taking Turbidity Water Samples 16  Sample Dilution Techniques 17
OPTIONS & SETUP
 Factory Default Settings 17  Averaging 18  Turbidity Options 20
Selecting Turbidity Units 20 Selecting a Turbidity Calibration Curve 22
 Setting Clock 24  Setting Power Save 26  Setting Backlight Time 28  Factory Reset 30  Selecting Language 32
DATA LOGGING 34 CALIBRATION & ANALYSIS
Calibration 36
Turbidity Standards 36 Turbidity Calibration Procedure 37
 Analysis without Blanking Procedure 41  Analysis with Blanking Procedure 43
 Dilution Procedure 46  Preparation of Turbidity-Free Water 47  Testing Tips 48
TROUBLESHOOTING GUIDE
 Troubleshooting 50  Stray Light 50
GENERAL OPERATING INFORMATION
 Overview 51  The Keypad 52  The Display and Menus 52  Negative Results 54  Tubes and Chambers 54
MAINTENANCE
 Cleaning 55  Repairs 55  Meter Disposal 56
Refer to the Quick Start Guide for simplifi ed Calibration and Analysis procedures.
Refer to the Testing Guide for detailed Calibration and Analysis procedures for improving the accuracy of low range turbidity measurements.
4
GENERAL INFORMATION
 PACKAGING AND DELIVERY
Experienced packaging personnel at LaMotte Company assure adequate protection against normal hazards encountered in transportation of shipments.
After the product leaves LaMotte Company, all responsibility for safe delivery is assured by the transportation company. Damage claims must be fi led immediately with the transportation company to receive compensation for damaged goods.
 GENERAL PRECAUTIONS
READ THE INSTRUCTION MANUAL BEFORE ATTEMPTING TO SET UP OR OPERATE THE METER. Failure to do so could result in
personal injury or damage to the meter. The meter should not be used or stored in a wet or corrosive environment. Care should be taken to prevent water from wet tubes from entering the meter chamber.
NEVER PUT WET TUBES IN THE METER.
 SAFETY PRECAUTIONS
Read the label on all reagent containers. Some labels include precautionary notices and fi rst aid information. Certain reagents are considered potential health hazards and are designated with a * in the instruction manual. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or FAX. Additional information for all LaMotte reagents is available in the United States, Canada, Puerto Rico, and the US Virgin Islands from Chem-Tel by calling 1-800-255-3924. For other areas, call 813-248-0585 collect to contact Chem-Tel’s International access number. Each reagent can be identifi ed by the four-digit number listed on the upper left corner of the reagent label, in the contents list and in the test procedures.
5
 LIMITS OF LIABILITY
Under no circumstances shall LaMotte Company be liable for loss of life, property, profi ts, or other damages incurred through the use or misuse of their products.
 SPECIFICATIONS - 2020we/wi
Instrument Type: Nephelometer Standard: EPA 180.1, 2020we; ISO 7027, 2020wi Units of Measure: NTU (Nephelometric Turbidity Units) (2020we only)
FNU (Formazin Nephelometric Units) (2020wi only) ASBC (American Society of Brewing Chemists) EBC (European Brewery Convention)
Range: 0-4000 NTU, 0-4000 FNU,
0-10,500 ASBC, 0-150 EBC Range Selection: Automatic Resolution:
(display)
Accuracy: From 0-2.5 NTU/FNU the accuracy is ±0.05 NTU/
Detection Limit: 0.05 NTU/FNU Light Source: Tungsten lamp 2300°C ±50 °C, 2020we; IR LED
Detector Photodiode, centered at 90°, maximum peak 400-
Response Time: <2 seconds Signal
Averaging: Sample
Chamber: Sample: 10 mL in capped tube Display: Graphic Liquid Crystal Display Software: Auto Shut-off: 5, 10, 30 min, disabled
Languages: English, Spanish, French, Portuguese, Italian,
Temperature: Operation: 0–50 °C; Storage: -40–60 °C
0.01 NTU/FNU, 0–10.99 NTU/FNU Range
0.1 NTU/FNU, 11.0–109.9 NTU/FNU Range
1 NTU/FNU, 110–4000 NTU/FNU Range
FNU. From 2.5-100 NTU/FNU the accuracy is ±2%.
Above 100 NTU/FNU the accuracy is ±3%.
850 nm ±10 nm, spectral bandwidth 50 nm,
2020wi
600 nm, 2020we
Photodiode, centered at 90°, 2020wi
Yes
Accepts 25 mm fl at-bottomed test tubes
Calibration: Field adjustable, blank and 1 point
Data Logging: 500 points
Chinese, Japanese (Kana)
6
Operation
0–90 % RH, non-condensing Humidity Range:
Auto Shut-off: 5, 10, 30 min, disabled Waterproof: IP67 with USB port plug in place.
Power Source
: USB wall adapter, USB computer connection or
Lithium ion rechargeable battery 2200 mAH, 3.7V Battery: Charge Life: Approximately 380 tests with backlight
on to 1000 tests with backlight off. (Signal
averaging disabled).
Battery Life: Approximately 500 charges. Electrical
Provided on nameplate label Ratings:
Dimensions: (W x L x H) 8.84 x 19.05 x 6.35 cm; 3.5 x 7.5 x 2.2
inches Weight: 362 g, 13 oz (meter only) USB Interface mini B
CE Mark: The device complies to the product specifi cations for the Low
Voltage Directive.
 STATISTICAL & TECHNICAL DEFINITIONS RELATED
TO PRODUCT SPECIFICATIONS
Method Detection Limit (MDL): “The method detection limit (MDL) is defi ned as the minimum concentration of a substance that can be measured and reported with 99% confi dence that the analyte concentration is greater than zero and is determined from analysis of a sample in a given matrix containing the analyte.”
1
Note that, “As Dr. William Horwitz once stated, ‘In almost all cases when dealing with a limit of detection or limit of determination, the primary purpose of determining that limit is to stay away from it.’”
2
Accuracy: Accuracy is the nearness of a measurement to the accepted or true value.3 The accuracy can be expressed as a range, about the true value, in which a measurement occurs (i.e. ±0.5 ppm). It can also be expressed as the % recovery of a known amount of analyte in a determination of the analyte (i.e. 103.5 %).
Resolution: Resolution is the smallest discernible difference between any two measurements that can be made.
4
For meters this is usually how many decimal places are displayed. (i.e. 0.01). Note that the resolution many change with concentration or range. In some cases the resolution may be less than the smallest interval, if it is possible to make a reading that falls between calibration marks. A word of caution, that resolution has very little relationship to accuracy or precision. The resolution will always be less than the accuracy or precision but it is not a statistical measure of how well a method of analysis works. The resolution can be very, very good and the accuracy and precision can
7
be very bad! This is not a useful measure of the performance of a test method.
Repeatability: Repeatability is the within-run precision.5 A run is a single data set, from set up to clean up. Generally, one run occurs on one day. However, for meter calibrations, a single calibration is considered a single run or data set, even though it may take 2 or 3 days.
Reproducibility: Reproducibility is the between-run precision.
6
Detection Limit (DL): The detection limit (DL) for the 2020we/wi is defi ned as the minimum value or concentration that can be determined by the meter, which is greater than zero, independent of matrix, glassware, and other sample handling sources of error. It is the detection limit for the optical system of the meter.
1
CFR 40, part 136, appendix B
2
Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L
Vanatta, American Laboratory, Sept 2003, P. 31.
3
Skoog, D.A., West, D. M., Fundamental of Analytical Chemistry, 2nd ed.,
Holt Rinehart and Winston, Inc, 1969, p. 26.
4
Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L
Vanatta, American Laboratory, Sept 2003, P. 34.
5
Jeffery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of
Quantitative Chemical Analysis, 5
th
ed., Longman Scientifi c & Technical,
1989, p. 130.
6
Jeffery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of Quantitative Chemical Analysis, 5th ed., Longman Scientifi c & Technical, 1989, p. 130
88
 CONTENTS & ACCESSORIES
2020we Kit EPA Version Code 1970-EPA
Contents Code Code
0 NTU Standard, 60 mL 1480 1480 1 NTU/FNU Standard, 60 mL 1450 1453 10 NTU/FNU Standard, 60 mL 1451 1454 Water Sample Bottle, 60 mL 0688 0688 Tubes, 6 — Cable, USB, 3 ft. 1720 1720 USB Wall Adapter 1721 1721
Accessories
Code Description
1452 100 NTU Standard, 60 mL (EPA) 1455 100 FNU Standard, 60 mL (ISO) 0290-6 Tubes, Code 0290, Set of 6 3-0038 Replacement Chamber 4185 Turbidity-Free Water Kit 2-2097 Filters, 0.1 micron, Pack of 50 1901-CD SMARTLink 3 Software 5-0132 Car Charger
2020wi Kit ISO Version Code 1970-ISO
99
 EPA COMPLIANCE
The 2020we meter meets or exceeds EPA design specifi cations for NPDWR and NPDES turbidity monitoring programs as specifi ed by the USEPA method 180.1.
 ISO Compliance
This 2020wi meter meets or exceeds ISO design criteria for quantitative methods of turbidity using optical turbidimeters as specifi ed by ISO
7027.
 CE COMPLIANCE
The 2020we and 2020wi meters have been independently tested and have earned the European CE Mark of compliance for electromagnetic compatibility and safety. To view certifi cates of compliance, go to the LaMotte website at www.lamotte.com.
NOTE: The device complies to the product specifi cations for the Low Voltage Directive.
 IP67 COMPLIANCE
The 2020we/wi meets IP67 standards for protection against dust and immersion only when the USB port plug is in place. Documentation is available at www.lamotte.com.
 WARRANTY
LaMotte Company warrants this instrument to be free of defects in parts and workmanship for 2 years from the date of shipment. If it should become necessary to return the instrument for service during or beyond the warranty period, contact our Technical Service Department at 1-800-344-3100 for a return authorization number or visit www.lamotte.com for troubleshooting help. The sender is responsible for shipping charges, freight, insurance and proper packaging to prevent damage in transit. This warranty does not apply to defects resulting from action of the user such as misuse, improper wiring, operation outside of specifi cation, improper maintenance or repair, or unauthorized modifi cation. LaMotte Company specifi cally disclaims any implied warranties or merchantability or fi tness for a specifi c purpose and will not be liable for any direct, indirect, incidental or consequential damages. LaMotte Company’s total liability is limited to repair or replacement of the product. The warranty set forth above is inclusive and no other warranty, whether written or oral, is expressed or implied.
 REGISTER YOUR METER
To register your meter with the LaMotte Service Department, go to www.lamotte.com and choose SUPPORT on the top navigation bar.
10
COMPUTER CONNECTION
 PC LINK
The 2020we/wi may be interfaced with any Windows-based computer by using the LaMotte SMARTLink 3 Program and USB Cable. The program will store test information and results in a database. To transfer data from the meter to a computer, plug the smaller end of the USB cable (USB mini B connector) into the meter and the larger end of the USB cable (USB Type A connector) into a USB port on a computer. The 2020we/ wi will send the following data: test name, wavelength, concentration, transmittance, absorbance, sample, blank, time of test, and date of test.
 OUTPUT
USB
 COMPUTER CONNECTION
USB Type A, USB mini B, Order Cable Code 1720.
BATTERY/AC OPERATION
The 2020we/wi may be operated on battery power or using USB wall adapter or USB computer connection. If using the meter as a bench top unit, use the wall adapter if possible to extend the battery life. The meter will remain on when the USB adapter is used.
To charge the battery with the wall adapter, plug the smaller end of the USB cable (USB mini B connector) into the meter and the larger end of the USB cable (USB Type A connector) into the wall adapter. Plug the wall adapter into an AC outlet. Reinsert the USB port plug after charging.
To charge the battery from a computer, plug the smaller end of the USB cable (USB mini B connector) into the meter and the larger end of the USB cable (USB Type A connector) into a USB port on a computer. Reinsert the USB port plug after charging.
The battery icon will show no bars and fl ash when the unit fi rst turns on. Then the indicator will indicate the battery status by showing 0, 1, 2, 3 or 4 bars.
It will take 5 hours to fully charge a low battery. The battery icon will ash when the battery is charging. The battery icon will show four bars and stop fl ashing when it is fully charged. The charging circuit will automatically switch to a fl oat charge when the battery is fully charged. The charger may remain connected. Some computers will NOT supply power to their USB ports during standby operation. The wall adapter will charge the unit continuously.
The battery icon will show no bars and continuously fl ash if the battery is getting low but the unit will still operate normally. A “Low Battery” message on the status bar of the display will replace the time when the battery voltage is too low for proper operation and accuracy may be degraded. A “Shutdown Low Batt” message on the display will appear for a few seconds before the power is switched off when the battery is too low to operate the unit.
11
To extend the battery life:
• Shut down the unit with the power switch when not taking measurements or use the power save option to have the unit automatically turn off after 5 minutes.
• Store the unit in a cool dry place.
• Fully charge the battery before storing the unit for extended periods of time.
• Limit backlight use. The unit consumes 3X normal power with the backlight on. Set the backlight time option to 10 seconds, or select “Button Control” and keep the backlight off.
Battery replacement: The lithium-ion battery used in this unit should last for many years with normal use. When it no longer powers the unit long enough to meet testing requirements it will need to be replaced. Lithium-ion batteries that are properly charged and stored do not usually lose all capacity; they just have less capacity after hundreds of charge cycles. This unit uses a custom battery assembly that is only available from LaMotte Company. Battery replacement must be performed at a LaMotte authorized repair facility. The water resistant housing of this meter should not be opened by the user. Contact LaMotte Company by phone (1-800-344-3100) or email (tech@lamotte.com) for a return authorization number.
12
TURBIDITY
 WHAT IS TURBIDITY?
Turbidity is an aggregate property of the solution, which is water in most cases. Turbidity is not specifi c to the types of particles in the water. The particles could be suspended or colloidal matter, and they can be inorganic, organic or biological. At high concentrations, turbidity is perceived as cloudiness, haze or an absence of clarity in the water. Turbidity is an optical property that results when light passing through a liquid sample is scattered. The scattering of light results in a change in the direction of the light passing through the liquid. This is most often caused when the light strikes particles in solution and is scattered backward, sideways and forward. If the turbidity is low, much of the light will continue in the original direction. Light scattered by the particles allows the particle to be ”seen” or detected in solution just as sunlight allows dust particles in the air to be seen.
In the past 10 years, turbidity has become more than just a measure of water clarity. Because of the emergence of pathogens such as Cryptosporidium and Giardia, turbidity now holds the key to assuring proper water fi ltration. In 1998, the EPA published the IESWTR (interim enhanced surface water treatment rule) mandating turbidities in combined fi lter effl uent to read at or below 0.3 NTU. By doing so, the EPA hoped to achieve a 2 log (99%) removal of Cryptosporidium. There is presently consideration to lower this to 0.1 NTU. The trend has been to check the calibration of on-line turbidimeters with hand-held fi eld units. The optical design and low detection limit of the 2020we/wi allows very accurate readings for such calibrations.
The meter also allows the user to choose the units of measure for expressing turbidity. While nephelometric turbidity unit (NTU) has been the standard for years, FNU (formazin nephelometric unit) and FAU (formazin attenuation unit) are now being used in ISO 7027 units. American Society of Brewing Chemists (ASBC) units and European Brewery Convention (EBC) units allow the brewing industry to check process waters.
 HOW IS TURBIDITY MEASURED?
Turbidity is measured by detecting and quantifying the scattering of light in water (solution). Turbidity can be measured in many ways. There are visual methods and instrumental methods. Visual methods are more suitable for samples with high turbidity. Instrumental methods can be used on samples with both high and low levels of turbidity.
Two visual methods are the Secchi Disk method and the Jackson Candle method. The Secchi Disk method is often used in natural waters. A black and white Secchi Disk is lowered into the water until it can no longer be seen. It is then raised until it can be seen again. The average of these two distances is known as the “Secchi Depth”. The Jackson Candle method uses a long glass tube over a standard candle. Water is added or removed from the tube until the candle fl ame becomes indistinct. The depth of the water measured with a calibrated scale is
13
reported as Jackson Turbidity Units (JTU). The lowest turbidity that can be determined with this method is about 25 NTU. There are two common methods for instruments to measure turbidity. Instruments can measure the attenuation of a light beam passing through a sample and they can measure the scattered light from a light beam passing through a sample. In the attenuation method, the intensity of a light beam passing through a turbid sample is compared with the intensity passing through a turbidity-free sample at 180° from the light source. This method is good for highly turbid samples. The most common instrument for measuring scattered light in a water sample is a nephelometer. A nephelometer measures light scattered at 90° to the light beam. Light scattered at other angles may also be measured, but the 90° angle defi nes a nephelometric measurement. The light source for nephelometric measurements can be one of two types to meet EPA or ISO specifi cations. The EPA specifi es a tungsten lamp with a color temperature of 2,200–3,000 K. The units of measurement for the EPA method are nephelometric turbidity units (NTU). The ISO specifi es a light emitting diode (LED) with a wavelength of 860 ± 30 nm and a spectral bandwidth less than or equal to 60 nm. The units of measurement for the ISO method are formazin nephelometric units (FNU). The 2020we meets the EPA specifi cation and the 2020wi meets the ISO specifi cation. The nephelometric method is most useful for low turbidity.
The 2020we/wi is a nephelometer that is capable of measuring turbidity by both the attenuation method and the nephelometric method. It uses a detector placed at 180° to the light source for high turbidity samples. It uses a detector placed at 90° to the light source for the nephelometric method for low turbidity samples. The 2020we/wi has a signal averaging option to improve the stability of readings on low turbidity samples.
The 2020we/wi has two different turbidity calibrations, formazin and Japan Standard. The formazin calibration is the EPA and ISO approved method of calibrating nephelometers. This calibration can be used with user prepared formazin standards or commercially purchased formazin standards. LaMotte Company approved AMCO
TM
standards labeled for use with the 2020we/wi can also be used with the formazin calibration. Stablcal® standards below 50 NTU should not be used to calibrate the 2020we/wi.
The Japan Standard calibration is a calibration for a Japanese Water Works standard. It is based on Japanese formulated polystyrene turbidity standards. This calibration should only be used to meet Japanese Water Works requirements. The Japanese polystyrene standards can only be purchased in Japan. Formazin, AMCO and Stablcal
®
standards cannot be used with this calibration.
14
 TURBIDITY UNITS
Traditionally, turbidimeters designed for use in the United State were made to the specifi cations of EPA Method 180.1. This method defi ned the NTU, nephelometric turbidity unit, as a unit to measure turbidity in the range of 0 – 40 NTU using a nephelometer. According to the EPA a nephelometer was a turbidimeter that measured turbidity with a 90° detector. Also, if the turbidity was greater than 40 NTU, a dilution was necessary to bring the sample into the 0 – 40 NTU range. Today, many turbidimeters have additional detectors which increase the range of the turbidity measurement, eliminate interferences and generally improve the performance. Currently, many turbidimeters are capable of measuring above 40 NTU by using detectors other than a 90° detector. Even though they may use a 180° detector to measure the light that is attenuated by high turbidity samples they may continue to report the results as NTU.
Recently there has been an effort to use the units of turbidity measurements to indicate which type of detector and light source was used. For EPA compliant meters, measurements made with a 90° degree detector and an incandescent white light source are reported as NTU. When an attenuation measurement is made with a 180°detector, using the same meter, the results are reported as AU, attenuation units. ISO Method 7027, which specifi es a 860 nm light source, also uses two turbidity units. When the 90° degree detector is used, the results are reported as FNU, formazin nephelometric units. With an attenuation measurement made with a 180°detector, the results are reported as FAU, formazin attenuation units. It should be noted that all units are numerically equivalent if the meters are calibrated to formazin and that the units only designate which detector was used to make the measurement. For example, 1 NTU = 1 AU = 1FNU = 1FAU.
15
Acronyms Defi nitions Notes
NTU Nephelometric
Turbidity Units
FNU Formazin
Options/Set Up
Nephelometric Units
AU Attenuation
Units
FAU Formazin
Attenuation Units
ASBC American
Society of Brewing Chemists
EBC European
Brewery Convention
Regulatory Method
Incandescent white
EPA 180.1 light between 400 and 600 nm, 90° detection, 2020we
IR LED (usually) 860
ISO 7027 nm, bandwidth less than 60 nm, 90° detection, 2020wi
Incandescent white light between 400 and
Not
applicable 600 nm, 180° detection, 2020we
IR LED (usually) 860
ISO 7027 nm, bandwidth less than 60 nm, 180° detection, 2020wi
2020we/wi Not
applicable
2020we/wi Not
applicable
The 2020we and 2020wi each use two turbidity units. The 2020we reports the result as NTU when the 90° detector is used and AU when the 180° detector is used. The 2020wi reports the result as FNU when the 90° detector is used and FAU when the 180° detector is used.
 TAKING TURBIDITY WATER SAMPLES
Clean plastic or glass containers may be used for turbidity samples. Ideally, samples should be tested soon after collection and at the same temperature as when collected.
16
 SAMPLE DILUTION TECHNIQUES
If a test result is out of the range of the meter, it must be diluted. The test should then be repeated on the diluted sample. The following table gives quick reference guidelines for dilutions of various proportions.
Options/Set Up
Amount of Sample Deionized Water to Bring
Final Volume to 10 mL 10 mL 0 mL 1 5 mL 5 mL 2
2.5 mL 7.5 mL 4 1 mL 9 mL 10
0.5 mL 9.5 mL 20
All dilutions are based on a fi nal volume of 10 mL, so several dilutions will require small volumes of the water sample. Graduated pipets should be used for all dilutions. If volumetric glassware is not available, dilutions can be made with the colorimeter tube. Fill the tube to the 10 mL line with the sample and then transfer it to another container. Add 10 mL volumes of deionized water to the container and mix. Transfer 10 mL of the diluted sample to the colorimeter tube and follow the test procedure. Repeat the dilution and testing procedures until the result falls within the range of the calibration. Multiply the test result by the dilution factor. For example, if 10 mL of the sample water is diluted with three 10 mL volumes of deionized water, the dilution factor is four. The test result of the diluted sample should be multiplied by four.
Multiplication Factor
OPTIONS & SET UP
 FACTORY DEFAULT SETTINGS
Settings that have user options have been set at the factory to default settings.
The factory default settings are:
Averaging Disabled Turbidity Units NTU Turbidity Calibration Formazin Date Format MM-DD-YYYY Power Save 5 minutes Backlight 10 seconds Language English
17
 AVERAGING
The averaging option allows the user to average multiple readings. This option will improve the accuracy of samples with readings that may tend to drift with time. When the two, fi ve or ten measurement option has been selected the fi nal average is displayed. The default setting is disabled. To change the setting:
1. Press and briefl y hold
Options/Set Up
to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.
2. Press to scroll to
Options.
3. Press
ENTER
to select
Options.
Main Menu
Measure
Data Logging Options Run PC Link
12:00:00 001/500
Main Menu Measure Data Logging
Options
Run PC Link
12:00:00 001/500
Options Menu
Averaging
Turbidity Options Set Clock Set PWR Save
12:00:00 001/500
4. Press Averaging.
ENTER
to select
Averaging Menu
Disabled
2 Measurements 5 Measurements 10 Measurements
12:00:00 001/500
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