Page 1
2
OPERATOR’S
MANUAL
2000-02-MN
www.GlobalTestSupply.com
05.02.16
Page 2
CONTENTS
GENERAL INFORMATION
Packaging & Delivery ............................................................................................................... 4
General Precautions ................................................................................................................ 4
Safety Precautions .................................................................................................................. 4
Limits of Liability ...................................................................................................................... 5
Warranty ...................................................................................................................................... 5
Specifi cations ........................................................................................................................... 6
Statistical and Technical Defi nitions
Related to Product Specifi cations ........................................................................................ 6
Contents and Accessories ...................................................................................................... 8
EPA Compliance ........................................................................................................................ 8
CHEMICAL TESTING
Water Sampling for Chemical Analysis ............................................................................... 9
Filtration ................................................................................................................................... 10
An Introduction to Colorimetric Analysis & Spectroscopy ........................................... 11
Reagent Blank ......................................................................................................................... 12
Spectrophotometer Tubes ................................................................................................... 12
Selecting an Appropriate Wavelength .............................................................................. 12
Calibration Curves .................................................................................................................. 13
Preparing Dilute Standard Solutions ................................................................................. 15
Standard Additions ................................................................................................................ 15
Sample Dilution Techniques & Volumetric Measurements ......................................... 16
Interferences ........................................................................................................................... 17
Stray Light Interference ....................................................................................................... 17
OPERATION OF THE SMART Spectro 2 SPECTROPHOTOMETER
Overview .................................................................................................................................... 18
Power Source ........................................................................................................................... 18
Components ............................................................................................................................ 19
Quick Start ............................................................................................................................... 20
GENERAL OPERATING PROCEDURES
The Keypad ............................................................................................................................... 23
Sample Holders ....................................................................................................................... 23
The Display & the Menus ...................................................................................................... 24
SYSTEM SETUP
Dark Current ..............................................................................................................................25
Calibrate WL ..............................................................................................................................26
BT Printer....................................................................................................................................28
Edit Clock ....................................................................................................................................33
Store Method.............................................................................................................................35
Beeper ON/OFF .........................................................................................................................38
Correction WL ...........................................................................................................................40
Language ...................................................................................................................................41
Reset to Default Settings .......................................................................................................43
www.GlobalTestSupply.com
Page 3
INITIALIZATION 45
PROGRAMMED TESTS
Introduction ............................................................................................................................. 46
Sequences of Tests ................................................................................................................ 47
General Testing Procedures ................................................................................................ 48
SETUP & EDIT SEQUENCES & USER TESTS
Edit a Sequence ...................................................................................................................... 51
Adding or Deleting Tests ....................................................................................................... 52
Edit User Tests ......................................................................................................................... 58
Naming the Test ..................................................................................................... 60
Selecting the Wavelength .................................................................................... 62
Entering a New Calibration .................................................................................. 64
Selecting the Numerical Format of the Result ............................................... 68
MEASURING IN THE %T/ABS MODE 70
STORE, DELETE AND PRINT DATA
Store Data ..................................................................................................................................73
Delete Data ................................................................................................................................74
Print Data ...................................................................................................................................76
PC LINK
Output ....................................................................................................................................... 76
Send 25Group Data .................................................................................................................76
Send All Data .............................................................................................................................76
Updata Programmed Tests ...................................................................................................76
BATTERY OPERATION
Charging the Battery Pack .................................................................................................... 76
Running the SMART Spectro 2 Using the Battery .......................................................... 77
MAINTENANCE
Cleaning .................................................................................................................................... 77
Lamp Replacement and Realignment .............................................................................. 77
Halogen Lamp Replacement.................................................................................................78
Clock Battery ........................................................................................................................... 52
Replacing the RAM Battery ....................................................................................81
Assembling the Housing Top and Bottom ..........................................................81
Meter Disposal ......................................................................................................................... 82
TROUBLESHOOTING GUIDE
Error Messages ....................................................................................................................... 82
Helpful Hints ............................................................................................................................ 83
SMART Spectro 2 TEST PROCEDURES
APPENDIX
www.GlobalTestSupply.com
Page 4
GENERAL INFORMATION
PACKAGING & DELIVERY
Experienced packaging personnel at LaMotte Company assure adequate protection
against normal hazards encountered in transportation of shipments. After the
product leaves the manufacturer, all responsibility for its safe delivery is assured
by the transportation company. Damage claims must be fi led immediately with the
transportation company to receive compensation for damaged goods.
Should it be necessary to return the instrument for repair or servicing, pack instrument
carefully in suitable container with adequate packing material. A return authorization
number must be obtained from LaMotte Company by calling 1-800-344-3100. Attach a
letter with the authorization number to the shipping carton which describes the kind of
trouble experienced. This valuable information will enable the service department to make
the required repairs more effi ciently.
GENERAL PRECAUTIONS
Before attempting to set up or operate this instrument it is important to read the
instruction manual. Failure to do so could result in personal injury or damage to the
equipment.
The SMART Spectro 2 should not be stored or used in a wet or corrosive environment.
Care should be taken to prevent water or reagent chemicals from wet spectrophotometer
tubes from entering the SMART Spectro 2 chamber.
NEVER PUT WET TUBES IN SPECTROPHOTOMETER.
SAFETY PRECAUTIONS
Read the labels on all LaMotte reagent containers prior to use. Some containers include
precautionary notices and fi rst aid information. Certain reagents are considered
hazardous substances and are designated with a * in the instruction manual. Safety Data
Sheets (SDS) available at www.lamotte.com. Read the accompanying SDS before using
these reagents. Additional emergency information for all LaMotte reagents is available 24
hours a day from the National Poison Control Center, 1-800-222-1222 or by contacting
the 24 hour emergency line for ChemTel 1-800-255-3924 (USA, Canada, Puerto Rico);
locations outside the North American continent 813-248-0585. Be prepared to supply
the name and four-digit LaMotte code number found on the container label or at the top of
the SDS or in the contents list for the test procedure. LaMotte reagents are registered with
a computerized poison control information system available to all local poison control
centers.
Keep equipment and reagent chemicals out of the reach of young children.
4 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 5
LIMITS OF LIABILITY
Under no circumstances shall LaMotte Company be liable for loss of life, property, profi ts,
or other damages incurred through the use or misuse of their products.
WARRANTY
LaMotte Company warrants this instrument to be free of defects in parts and
workmanship for 2 years from the date of shipment. If it should become necessary to
return the instrument for service during or beyond the warranty period, contact our
Technical Service Department at 1-800-344-3100 for a return authorization number or
visit www.lamotte.com for troubleshooting help. The sender is responsible for shipping
charges, freight, insurance and proper packaging to prevent damage in transit. This
warranty does not apply to defects resulting from action of the user such as misuse,
improper wiring, operation outside of specifi cation, improper maintenance or repair,
or unauthorized modifi cation. LaMotte Company specifi cally disclaims any implied
warranties or merchantability or fi tness for a specifi c purpose and will not be liable for any
direct, indirect, incidental or consequential damages. LaMotte Company’s total liability is
limited to repair or replacement of the product. The warranty set forth above is inclusive
and no other warranty, whether written or oral, is expressed or implied.
To register your meter with the LaMotte Service Department go to www.lamotte.com and
choose SUPPORT on the top navigation bar.
SMART Spectro 2 Operator’s Manual 04.16 5
www.GlobalTestSupply.com
Page 6
SPECIFICATIONS
INSTRUMENT TYPE: Single beam spectrophotometer
Wavelength Range 350-1000 nm
Optical System Single Beam, Grating 1200 lines/mm
Wavelength Accuracy and
Repeatability
Photometric Accuracy ± 0.5%T
Bandwidth 4 nm
Transmittance Range 0-125%T
Absorbance Range -0.3-2.500A
Stray Light
Display LCD
Light Source Halogen 12V / 10W
Power Supply Power Adapter 12 VDC
Power Consumption 50W
Dimension (L x W x H) 360 x 280 x 180 mm
Net Weight 13.2 lbs, 6 kg
Communication Port USB
± 2 nm
0.4%T@360 nm
STATISTICAL AND TECHNICAL DEFINITIONS RELATED TO PRODUCT
SPECIFICATIONS
Method Detection Limit (MDL): “The method detection limit (MDL) is defi ned as the
minimum concentration of a substance that can be measured and reported with 99%
confi dence that the analyte concentration is greater than zero and is determined from
analysis of a sample in a given matrix containing the analyte.”1 Note that, “As Dr. William
Horwitz once stated, ‘In almost all cases when dealing with a limit of detection or limit of
determination, the primary purpose of determining that limit is to stay away from it.’”2
1. CFR 40, part 136, appendix B
2. Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and
L Vanatta, American Laboratory, Sept 2003, P. 31.
Precision: Precision is the numerical agreement between two or more measurements.3
The precision can be reported as a range for a measurement (diff erence between the
min and max). It can also be reported as the standard deviation or the relative standard
deviation. It is a measure of how close together the measurements are, not how close they
are to the correct or true value. The precision can be very good and the accuracy very bad.
This is a useful measure of the performance of a test method.
6 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 7
3. Skoog, D.A., West, D. M., Fundamental of Analytical Chemistry, 2nd ed., Holt Rinehart and
Winston, Inc, 1969, p. 26.
Accuracy: Accuracy is the nearness of a measurement to the accepted or true value.4
The accuracy can be expressed as a range, about the true value, in which a measurement
occurs (i.e. ±0.5 ppm). It can also be expressed as the % recovery of a know amount of
analyte in a determination of the analyte (i.e. 103.5 %). This is a useful measure and what
most customers are interested in when they want to know about the performance of a test
method.
4. Skoog D.A., West D. M., Fundamental of Analytical Chemistry, 2nd ed., Holt Rinehart and
Winston, Inc, 1969, p. 26.
Resolution: Resolution is the smallest discernible diff erence between any two
measurements that can be made.5 For meters this is usually how many decimal places
are displayed. (i.e. 0.01). For titrations and various comparators it is the smallest interval
the device is calibrated or marked to (i.e. 1 drop = 10 ppm, 0.2 ppm for a DRT, or ±half
a unit diff erence for an octaslide or color chart). Note that the resolution many change
with concentration or range. In some cases the resolution may be less than the smallest
interval, if it is possible to make a reading that falls between calibration marks. This
is often done with various comparators. One caveat is, that resolution has very little
relationship to accuracy or precision. The resolution will always be less than the accuracy
or precision but it is not a statistical measure of how well a method of analysis works. The
resolution can be very very good and the accuracy and precision can be very, very bad! This
is not a useful measure of the performance of a test method.
5. Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and
L Vanatta, American Laboratory, Sept 2003, P. 34.
Sensitivity: Sensitivity is the resolution based on how this term is used in LaMotte
catalogs. This term is not listed in any of the references. Sometimes it is used for
detection limit. It is a confusing term and should be avoided.
Repeatability: Repeatability is the within-run precision.6 A run is a single data set, from
set up to clean up. Generally, one run occurs on one day. However, for meter calibrations,
a single calibration is considered a single run or data set, even though it may take 2 or 3
days.
6. Jeff ery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of Quantitative
Chemical Analysis, 5th ed., Longman Scientifi c & Technical, 1989, p. 130.
Reproducibility: Reproducibility is the between-run precision.7
7. Jeff ery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of Quantitative
Chemical Analysis, 5th ed., Longman Scientifi c & Technical, 1989, p. 130.
SMART Spectro 2 Operator’s Manual 04.16 7
www.GlobalTestSupply.com
Page 8
CONTENTS AND ACCESSORIES
Contents
SMART Spectro 2 Spectrophotometer Power Cable
Test Tubes, with Caps AC Adapter
Sample Cell Holder, Universal SMART Spectro 2 Quick Start Guide
Sample Cell Holder, 10 mm Square SMART Spectro 2 Manual
NOTE: The Battery Pack and Battery Charger are not included and must be purchased
separately.
Accessories
Battery Pack (rechargeable) Code 2000-BP
Battery Charger Code 2000-BC
Carrying Case Code 2000-CS
Replacement Test Tubes with caps (6) Code 0290-6
Cuvettes Code 29653-10
Bluetooth Mobile Printer Code 5-0066
SMARTLink Software with Cable (compact disk) For more information
contact LaMotte
EPA COMPLIANCE
The SMART Spectro 2 is an EPA-Accepted instrument. EPA-Accepted means that the
instrument meets the requirements for instrumentation as found in test procedures that
are approved for the National Primary Drinking Water Regulations (NPDWR) or National
Pollutant Discharge Elimination System (NPDES) compliance monitoring programs.
EPA-Accepted instruments may be used with approved test procedures without additional
approval.
8 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 9
CHEMICAL TESTING
WATER SAMPLING FOR CHEMICAL ANALYSIS
Taking Representative Samples
The underlying factor to be considered for any type of water sampling is whether or not the
sample is truly representative of the source. To properly collect a representative sample:
• Sample as frequently as possible.
• Collect a large sample or at least enough to conduct whatever tests are necessary.
• Make a composite sample for the same sampling area.
• Handle the sample in such a way as to prevent deterioration or contamination
before the analysis is performed.
• Perform analysis for dissolved gases such as dissolved oxygen, carbon dioxide, and
hydrogen sulfi de immediately at the site of sampling. These factors, as well as
samples for pH testing, cannot be stored for later examination.
• Make a list of conditions or observations which may aff ect the sample. Other
considerations for taking representative samples are dependent upon the
source of the sample. Taking samples from surface waters involves diff erent
considerations than taking samples from impounded and sub-surface waters.
Sampling of Open Water Systems
Surface waters, such as those found in streams and rivers, are usually well mixed. The
sample should be taken downstream from any tributary, industrial or sewage pollution
source. For comparison purposes samples may be taken upstream and at the source of
the pollution.
In ponds, lakes, and reservoirs with restricted fl ow, it is necessary to collect a number of
samples in a cross section of the body of water, and where possible composite samples
should be made to ensure representative samples.
To collect samples from surface waters, select a suitable plastic container with a tight
fi tting screw cap. Rinse the container several times with the sample to be tested, then
immerse the container below the surface until it is fi lled to overfl owing and replace the
cap. If the sample is not to be tested immediately, pour a small part of the sample out
and reseal. This will allow for any expansion. Any condition which might aff ect the sample
should be listed.
Sub-surface sampling is required to obtain a vertical profi le of streams, lakes, ponds, and
reservoirs at specifi c depths. This type of sampling requires more sophisticated sampling
equipment.
For dissolved oxygen studies, or for tests requiring small sample sizes, a Water Sampler
(LaMotte Code 1060) will serve as a sub-surface or in-depth sampler. This weighted
device is lowered to the sampling depth and allowed to rest at this depth for a few minutes.
The water percolates into the sample chamber displacing the air which bubbles to the
surface. When the bubbles cease to rise, the device has fl ushed itself approximately
SMART Spectro 2 Operator’s Manual 04.16 9
www.GlobalTestSupply.com
Page 10
fi ve times and it may be raised to the surface for examination. The inner chamber of the
sampling device is lifted out and portions of the water sample are carefully dispensed for
subsequent chemical analysis.
A Snap-Plunger Water Sampler (LaMotte Code 1077) is another “in-depth” sampling
device which is designed to collect large samples which can be used for a multitude of
tests. Basically, this collection apparatus is a hollow cylinder with a spring loaded plunger
attached to each end. The device is cocked above the surface of the water and lowered
to the desired depth. A weighted messenger is sent down the calibrated line to trip the
closing mechanism and the plungers seal the sample from mixing with intermediate
layers as it is brought to the surface. A special drain outlet is provided to draw off samples
for chemical analysis.
Sampling of Closed System
To obtain representative samples from confi ned water systems, such as pipe lines, tanks,
vats, fi lters, water softeners, evaporators and condensers, diff erent considerations are
required because of chemical changes which occur between the inlet and outlet water.
One must have a basic understanding of the type of chemical changes which occur for the
type of equipment used. Also, consideration should be given to the rate of passage and
retaining time for the process water.
Temperature changes play an important part in deciding exactly what test should be
performed. Process water should be allowed to come to room temperature, 20–25°C,
before conducting any tests.
When drawing off samples from an outlet pipe such as a tap, allow sample to run for
several minutes, rinsing the container several times before taking the fi nal sample. Avoid
splashing and introduction of any contaminating material.
FILTRATION
When testing natural waters that contain signifi cant turbidity due to suspended solids
and algae, fi ltration is an option. Reagent systems, whether EPA, Standard Methods,
LaMotte or any others, will generally only determine dissolved constituents. Both EPA and
Standard Methods suggest fi ltration through a 0.45 micron fi lter membrane, to remove
turbidity, for the determination of dissolved constituents.** To test for total constituents,
organically bound and suspended or colloidal materials, a rigorous high temperature acid
digestion is necessary.
**LaMotte off ers a fi ltering apparatus: syringe assembly (Code 1050) and membrane
fi lters, 0.45 micron, (Code 1103).
10 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 11
AN INTRODUCTION TO COLORIMETRIC ANALYSIS &
SPECTROSCOPY
Most test substances in water are colorless and undetectable to the human eye. To test
for their presence we must fi nd a way to “see” them. The LaMotte SMART Spectro 2 can
be used to measure any test substance that is itself colored or can be reacted to produce
a color. In fact a simple defi nition of colorimetry is “the measurement of color” and a
colorimetric method is “any technique used to evaluate an unknown color in reference to
known colors”. In a colorimetric chemical test the intensity of the color from the reaction
must be proportional to the concentration of the substance being tested. Some reactions
have limitations or variances inherent to them that may give misleading results. Many
such interferences are discussed with each particular test instruction. In the most basic
colorimetric method the reacted test sample is visually compared to a known color
standard. However, accurate and reproducible results are limited by the eyesight of the
analyst, inconsistencies in the light sources, and the fading of color standards.
To avoid these sources of error, a colorimeter or spectrophotometer can be used to
photoelectrically measure the amount of colored light absorbed by a colored sample in
reference to a colorless sample (blank).
White light is made up of many diff erent colors or wavelengths of light. A colored sample
typically absorbs only one color or one band of wavelengths from the white light. Only
a small diff erence would be measured between white light before it passes through a
colored sample versus after it passes through a colored sample. The reason for this is
that the one color absorbed by the sample is only a small portion of the total amount of
light passing through the sample. However, if we could select only that one color or band
of wavelengths of light to which the test sample is most sensitive, we would see a large
diff erence between the light before it passes through the sample and after it passes
through the sample.
The SMART Spectro 2 uses a quartz halogen lamp as the source of white light. The white
light passes through an entrance slit and is focused on a ruled grating consisting of
1200 lines/mm. The grating causes the light to be dispersed into various component
wavelengths. The monochromator design allows the user to select which specifi c
wavelength of interest will be passed through the exit slit and through the sample. The use
of mirrors and additional fi lters prevents light of undesired wavelengths (overtones, stray
light) from making it to the sample. A photodetector measures the amount of light which
passes through the sample.
The diff erence in the amount of monochromatic light transmitted through a colorless
sample (blank) and the amount of monochromatic light transmitted through a test
sample is a measurement of the amount of monochromatic light absorbed by the sample.
In most colorimetric tests the amount of monochromatic light absorbed is directly
proportional to the concentration of the test factor producing the color and the path
length through the sample. However, for a few tests the relationship is reversed and the
amount of monochromatic light absorbed is inversely proportional to the concentration of
the test factor.
The choice of the correct wavelength for testing is important. It is interesting to note that
the wavelength that gives the most sensitivity (lower detection limit) for a test factor
is the complementary color of the test sample. For example the Nitrate-Nitrogen test
SMART Spectro 2 Operator’s Manual 04.16 11
www.GlobalTestSupply.com
Page 12
produces a pink color proportional to the nitrate concentration in the sample (the greater
the nitrate concentration, the darker the pink color). A wavelength in the green region
should be selected to analyze this sample since a pinkish-red solution absorbs mostly
green light.
REAGENT BLANK
Some tests will provide greater accuracy if a reagent blank is determined to compensate
for any color or turbidity resulting from the reagents themselves. A reagent blank is
performed by running the test procedure on 10 mL of demineralized or deionized water.
Use sample water to SCAN BLANK. Insert the reacted reagent blank in the colorimeter
chamber and select SCAN SAMPLE. Note result of reagent blank. Perform the tests on the
sample water as described. Subtract results of reagent blank from all subsequent test
results.
NOTE: Some tests require a reagent blank to be used to SCAN BLANK.
SPECTROPHOTOMETER TUBES
Spectrophotometer tubes which have been scratched through excessive use should be
discarded and replaced with new ones. Dirty tubes should be cleaned on both the inside
and outside. Fingerprints on the exterior of the tubes can cause excessive light scattering
and result in errors. Handle the tubes carefully, making sure the bottom half of the tube is
not handled.
LaMotte Company makes every eff ort to provide high quality spectrophotometer tubes.
However, wall thicknesses and diameter of tubes may still vary slightly. This may lead to
slight variations in results (e.g. if a tube is turned while in the sample chamber, the reading
will likely change slightly). To eliminate this error put the tubes into the sample chamber
with the same orientation every time.
The tubes that are included with the spectrophotometer have an index mark to facilitate
this. If possible, use the same tube to SCAN BLANK and SCAN SAMPLE.
SELECTING AN APPROPRIATE WAVELENGTH
The most appropriate wavelength to use when creating a calibration curve is usually the
one which gives the greatest change from the lowest reacted standard concentration
to the highest reacted standard concentration. However, the absorbance of the highest
reacted standard concentration should never be greater than 2.0 absorbance units.
Scan the lowest and highest reacted standards at diff erent wavelengths using the %T/
ABS mode to fi nd the wavelength which gives the greatest change in absorbance without
exceeding 2.0 absorbance units. Use this wavelength to create a calibration curve.
A list of suggested wavelength ranges for the color of the reacted samples follows. Use
these as a starting point.
12 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 13
Sample Color Wavelength Range
Yellow 350-450
Yellow-Orange 450-490
Orange 490-510
Pink 510-570
Red 570-600
Green and Blue 600-750
CALIBRATION CURVES
The SMART Spectro 2 contains precalibrated tests for the LaMotte reagent systems. The
fi rst step in using a non-LaMotte reagent system with the SMART Spectro 2 is to create a
calibration curve for the reagent system. To create a calibration curve, prepare standard
solutions of the test factor and use the reagent system to test the standard solutions with
the SMART Spectro 2.
Plot the results (in ABS or %Transmittance) versus concentration to create a calibration
curve. The calibration curve may then be used to identify the concentration of an unknown
sample by testing the unknown, reading Absorbance or %T, and fi nding the corresponding
concentration from the curve. The linear range of the reagent system can be determined
and this information can be used to input a User Test into the SMART Spectro 2 (see EDIT
USER TESTS, page 58).
PROCEDURE
1. Prepare 5 or 6 standard solutions of the factor being tested. The concentration of
these standards should be evenly distributed throughout the range of the reagent
system, and should include a 0 ppm standard (distilled water). For instance, the
solutions could measure 0, 10%, 30%, 50%, 70%, and 90% of the system’s maximum
range.
2. Turn on the SMART Spectro 2. Select the appropriate %T/ABS wavelength from the
%T/ABS mode. Be sure to select the appropriate wavelength for the color produced by
the reagent system.
3. Use the unreacted 0 ppm standard to standardize the spectrophotometer by using it
to scan blank.
4. Following the individual reagent system instructions, react each standard solution
including 0 ppm. Record the reading and the standard solution concentration on a
chart. Readings can be recorded as percent transmittance (%T) or absorbance (A).
5. Plot results on graph paper or computer using any available plotting program. If
results are as %T versus concentration, semilog graph paper must be used. Plot
the standard solution concentrations on the horizontal, linear axis, and the %T on
the vertical, logarithmic axis. If results are as absorbance versus standard solution
SMART Spectro 2 Operator’s Manual 04.16 13
www.GlobalTestSupply.com
Page 14
concentration, simple linear graph paper can be used. Plot the standard solution
concentration on the horizontal axis, and the absorbance on the vertical axis.
6. After plotting the results, draw a line, or curve, of best fi t through the plotted points.
The best fi t may not connect the points. There should be approximately an equal
number of points above the curve as below the curve. Some reagent systems will
produce a straight line, while others produce a curve. Many computer spreadsheet
programs can produce the curve of best fi t by regression analysis of the standard
solution data.
A sample of each type of graph appears below:
14 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 15
PREPARING DILUTE STANDARD SOLUTIONS
Standard solutions should be prepared to create a calibration curve. Standard solutions
can be prepared by diluting a known concentrated standard by specifi ed amounts. A
chart or computer spreadsheet can be created to determine the proper dilutions. Use
volumetric fl asks and volumetric pipets for all dilutions.
1. In Column A – Record the maximum concentration of test as determined by the range
and path length.
2. In Column B – Record the percent of the maximum concentration the standard
solution will be.
3. In Column C – Calculate the fi nal concentration of the diluted standard solutions
by multiplying the maximum concentration (In Column A) by the % of maximum
concentration divided by 100. (C = A x ).
4. In Column D – Record the fi nal volume of the diluted sample (i.e. volume of volumetric
fl ask).
5. In Column E – Record the concentration of the original standard.
6. In Column F – Calculate the milliliters of original standard required (C x D/E = F).
A sample chart appears below:
A B C=A x B/100 D E F=C x D/E
Maximum
concentration
of test
10.0 ppm 90 9.0 ppm 100 mL 1000 ppm 0.90 mL
10.0 ppm 70 7.0 ppm 100 mL 1000 ppm 0.70 mL
10.0 ppm 50 5.0 ppm 100 mL 1000 ppm 0.50 mL
10.0 ppm 30 3.0 ppm 100 mL 1000 ppm 0.30 mL
10.0 ppm 10 1.0 ppm 100 mL 1000 ppm 0.10 mL
10.0 ppm 0 0 ppm 100 mL 1000 ppm 0 mL
% of Maximum
concentration
Final concentration of
Diluted Standard
Volume of
Standard
Concentration of
Original Standard
mL of
Original Standard
Required
STANDARD ADDITIONS
A common method to check the accuracy and precision of a test is by standard
additions. In this method a sample is tested to determine the concentration of the test
substance. A second sample is then “spiked” by the addition of a known quantity of the
test substance. The second sample is then tested. The determined concentration of the
spiked sample should equal the concentration of the fi rst plus the amount added with the
spike. The procedure can be repeated with larger and larger “spikes.” If the determined
concentrations do not equal the concentration of the sample plus that added with the
“spike”, then an interference may exist.
SMART Spectro 2 Operator’s Manual 04.16 15
www.GlobalTestSupply.com
Page 16
For example, a 10.0 mL water sample was determined to contain 0.3 ppm iron. To a
second 10.0 mL sample, 0.1 mL of 50 ppm iron standard was added. The concentration of
iron due to the “spike” was (0.10 mL x 50 ppm)/10.0 mL = 0.50 ppm. The concentration of
iron determined in the spiked sample should be 0.3 + 0.5 = 0.8 ppm iron.
(Note: any error due to the increased volume from the “spike” is negligible).
LaMotte off ers a line of calibration standards which can be used to generate calibration
curves and perform standard additions.
SAMPLE DILUTION TECHNIQUES & VOLUMETRIC MEASUREMENTS
If a test result using the SMART Spectro 2 gives an OUT OF RANGE message then the
sample concentration could be over range or under range. If it is over range, the sample
must be diluted. Then the test should be repeated on the diluted sample to obtain
a reading which is in the concentration range for the test. (Note: This is not true for
colorimetric determination of pH.)
Example:
Measure 5 mL of the water sample into a graduated cylinder. Add demineralized water
until the cylinder is fi lled to the 10 mL line. The sample has been diluted by one-half, and
the dilution factor is therefore 2. Perform the test procedure, then multiply the resulting
concentration by 2 to obtain the test result.
The following table gives quick reference guidelines on dilutions of various proportions. All
dilutions are based on a 10 mL volume, so several dilutions will require small volumes of
the water sample. Graduated pipets should be used for all dilutions.
Size of Sample Deionized Water to Bring
Volume to 10 mL
10 mL 0 mL 1
5 mL 5 mL 2
2.5 mL 7.5 mL 4
1 mL 9 mL 10
0.5 mL 0.5 mL 20
If the above glassware is not available, dilutions can be made with the spectrophotometer
tube. Fill the tube to the 10 mL line with the sample then transfer it to another container.
Add 10 mL volumes of demineralized water to the container and mix. Transfer back 10
mL of the diluted sample to the tube and follow the test procedure. Continue diluting and
testing until a reading, which is in the concentration range for the test, is obtained. Be
sure to multiply the concentration found by the dilution factor (the number of total 10 mL
volumes used).
Example:
10 mL of sample is diluted with three 10 mL volumes of demineralized water; the dilution
factor is four.
16 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Multiplication Factor
Page 17
INTERFERENCES
LaMotte reagent systems are designed to minimize most common interferences. Each
individual test instruction discusses interferences unique to that test. Be aware of
possible interferences in the water being tested.
The reagent systems also contain buff ers to adjust the water sample to the ideal pH
for the reaction. It is possible that the buff er capacity of the water sample may exceed
the buff er capacity of the reagent system and the ideal pH will not be obtained. If this is
suspected, measure the pH of a reacted distilled water reagent blank using a pH meter.
This is the ideal pH for the test. Measure the pH of a reacted water sample using the pH
meter. If the pH is signifi cantly diff erent from the ideal value, the pH of the sample should
be adjusted before testing.
Chlorine interferences can be removed with the use of glycine. Very high levels of
chloramines may interfere if the test result is not read immediately. Oxidized manganese
interferes but can be removed with arsenite. Bromine and iodine interferes but can be
removed with a thioacetamide blank correction.
Interferences due to high concentration of the substance being tested, can be overcome
by sample dilution (see page 16).
STRAY LIGHT INTERFERENCE
Normal indoor lighting causes no interference with the SMART Spectro 2. Always be sure
the sample chamber lid is closed when scanning blanks or samples.
SMART Spectro 2 Operator’s Manual 04.16 17
www.GlobalTestSupply.com
Page 18
OPERATION OF THE SMART Spectro 2
OVERVIEW
The SMART Spectro 2 is a portable, microprocessor controlled, direct reading, single beam
spectrophotometer. It has a 9 line liquid crystal display for alphabetical and numerical
messages. The operation is controlled with the keypad through menu driven software in
response to selections shown on the display.
The test library consists of over 80 LaMotte tests and 25 “User Tests”. The
spectrophotometer is also capable of running %T/Absorbance tests over the entire
wavelength range of 350 - 1000 nm. The LaMotte tests are precalibrated for LaMotte
reagent systems. The spectrophotometer displays the results of these tests directly in
units of concentration. The 25 “User Tests” may be used to enter additional calibrations.
All of these tests may be arranged in any of 3 sequences. These sequences can be
modifi ed a limitless number of times to meet changing testing needs.
The optics feature a halogen bulb as a light source with a minimum life expectancy of
1000 hours. The incident white light is dispersed into its component wavelengths by a
1200 lines/mm ruled grating. The microprocessor controls the positioning of the grating,
automatically positioning the grating to the correct wavelength for the test that has been
selected. The monochromatic light is passed through the sample cell and is detected by a
silicon photodiode.
The SMART Spectro 2 is powered by an AC adapter that automatically recognizes the input
voltage (110/220V) and converts it to the 12V needed to run the instrument. An optional
battery pack and battery charger are available for use where portability is important.
A USB port on the back of the spectrophotometer, and optional software, allows the
spectrophotometer to be interfaced with a Winows-based personal computer for real
time data acquisition and data storage. The data storage capacity is 1000 test results for
%T/ABS tests and up to 1000 test results for each quantitatve test. Due to its portability,
alternate power sources, and rugged construction, the SMART Spectro 2 is ideal for lab
and fi eld use.
POWER SOURCE
To use the SMART Spectro 2 with an AC power supply:
1. Plug the Power Supply into the AC Adapter socket on the back of the SMART Spectro 2.
2. Connect the Power Cable to the Power Supply and an electrical outlet.
To use the Battery Pack, see page 76.
18 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 19
COMPONENTS
Figure 1 shows a diagram of the SMART Spectro 2 and the components.
Lamp
Replacement Access:
For lamp replacement
and realignment
Chamber
25mm
Round Cell
in Universal
Adapter
Scroll
Between
Choices
Power Input Socket:
For 12V DC Power input
Make
Selections
Display
Between
Choices
USB Port
Scroll
10mm
Square Cell
in Adapter
Turn Off
Meter
Exit
Menus
Turn On
Meter
Figure 1
SMART Spectro 2 Operator’s Manual 04.16 19
www.GlobalTestSupply.com
Page 20
QUICK START
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
2. Press or to scroll to
PROGRAMMED TESTS.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
3. Press *ENTER to select PROGRAMED
TESTS.
20 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
PROGRAMMED TESTS 546 NM
SEQUENCE 1
SEQUENCE 2
SEQUENCE 3
ALL TESTS
EDIT
Page 21
4. Press or to scroll to
ALL TESTS.
PROGRAMMED TESTS 546 NM
SEQUENCE 1
SEQUENCE 2
SEQUENCE 3
ALL TESTS
EDIT
5. Press *ENTER to select ALL TESTS. ALL TESTS 546 NM
1 ALKALINITY-UDV
2 ALUMINUM
3 AMMONIA-N L F
4 AMMONIA-N L S
5 AMMONIA-N H
6 ARSENIC
7 BIGUANIDE
8 BORON
6. Press or to scroll to the desired
test.
ALL TESTS 546 NM
1 ALKALINITY-UDV
2 ALUMINUM
3 AMMONIA-N L F
4 AMMONIA-N L S
5 AMMONIA-N H
6 ARSENIC
7 BIGUANIDE
8 BORON
SMART Spectro 2 Operator’s Manual 04.16 21
www.GlobalTestSupply.com
Page 22
7. Press *ENTER to select the test. 2 ALUMINUM 535 NM
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
8. Insert the blank. Close lid. Press *ENTER
to scan the blank. BLANKING will
momentarily appear on the top line of the
display. The blank has been stored.
9. Insert the reacted sample. Close lid. Press
*ENTER to scan the sample. The result will
be displayed.
2 ALUMINUM 535 NM
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
2 ALUMINUM 535 NM
T = 13.5%T
A = 0.8707A
C = 0.26 ppm
STORE PRESS [ENTER]
After obtaining test results, scroll with or , and make another selection with
*ENTER. Press *ENTER to store th result to the Test Result List. If Store Method is
automatic test results will be stored automatically. To print the test results, scroll to Test
Result List. Press *ENTER to print.
After obtaining test results, scroll with or , and make another selection with
*ENTER. Press EXIT to escape to previous menu.
22 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 23
GENERAL OPERATING PROCEDURES
The operation of the SMART Spectro 2 is controlled by a microprocessor. The
microprocessor is programmed with menu driven software. A menu is a list of choices.
This allows a selection of various tasks for the spectrophotometer to perform, such as,
scan blank, scan sample, and edit test sequences. The keypad is used to make menu
selections which are viewed in the display. There are four selections accessible from the
MAIN MENU - PROGRAMMED TESTS, %T/ABS, PC LINK and SYSTEM SETUP.
THE KEYPAD
The keypad has 6 buttons which are used to perform specifi c tasks.
ON This button is used to turn the spectrophotometer on and begin
initialization.
This button will cause the display to scroll up in a list of menu choices. It will
move to the beginning of a list viewed in the display. It will auto scroll when
held down.
This button will cause the display to scroll down through a list of menu
choices. It will move to the end of a list viewed in the display. It will auto scroll
when held down.
*
ENTER
EXIT This button is an EXIT or ESCAPE button. When pressed, the display will EXIT
OFF This button turns the spectrophotometer off .
This button is used to select the menu choice indicated by the white bar on a
menu viewed in the display.
from the current menu and go to the previous menu. Pressing EXIT during
the initialization will skip the 15 minute warm up.
SAMPLE HOLDERS
The SMART Spectro 2 is supplied with two removable sample cell holders. Each holder
is secured in the chamber with a single screw. The square sample holder should be
positioned so the arrow on the top is pointing toward the left. The square sample holder
will hold 10 mm square cuvettes. The universal sample holder should be positioned with
the V-channel toward the right side of the chamber. The universal sample holder will hold
round tubes of varying diameters. When using the universal adapter, the tube should be
placed between the white roller on the spring-loaded arm and the v-channel on the righthand side of the adapter. Press the tube down on the white roller to retract the arm.
SMART Spectro 2 Operator’s Manual 04.16 23
www.GlobalTestSupply.com
Page 24
THE DISPLAY & THE MENUS
The display allows menu selections to be viewed and chosen. These choices instruct the
spectrophotometer to perform specifi c tasks. The menus are viewed in the display using a
general format which is followed from one menu to the next. Each menu is a list of choices
or selections.
There are nine lines in the display. The top line in each menu is a title or pertinent
instruction. The top line does not change unless a new menu is selected.
DISPLAY
TESTING MENU Title or Instruction
FIRST CHOICE
SECOND CHOICE Menu Choice Window
THIRD CHOICE
AND ANOTHER
AND ANOTHER
AND ANOTHER
AND ANOTHER
AND ANOTHER
AND SO ON
END OF LIST
Think of the menu choices as a vertical list in the display which moves up or down each
time an arrow button is pressed. This list or menu is viewed through a window, the menu
choice window, in the display. Pushing the arrow buttons brings another portion of the
menu into menu choice window. This is referred to as scrolling through the menu.
24 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 25
TESTING MENU TESTING MENU TESTING MENU
FIRST CHOICE SECOND CHOICE ANOTHER
SECOND CHOICE ANOTHER AND ANOTHER
ANOTHER AND ANOTHER AND ANOTHER
AND ANOTHER AND ANOTHER AND ANOTHER
AND ANOTHER AND ANOTHER AND ANOTHER
AND ANOTHER AND ANOTHER AND ANOTHER
AND ANOTHER AND ANOTHER AND ANOTHER
AND ANOTHER AND ANOTHER AND ANOTHER
AND SO ON AND SO ON AND SO ON
END OF LIST END OF LIST END OF LIST
A white bar, will start on the top line in the menu choice window. As the menu is scrolled
through, diff erent choices will be highlighted with the white bar. The white bar on the
display corresponds to the *ENTER button. Pushing the *ENTER button selects the menu
choice which is highlighted with the white bar in the menu choice window.
As described previously, the EXIT button allows an exit or escape from the current menu
and a return to the previous menu. This allows a rapid exit from an inner menu to the
MAIN MENU by repeatedly pushing the EXIT button. Pushing OFF at any time will turn the
spectrophotometer off .
SYSTEM SETUP
The System Setup Menu, found in the Main Menu, allows the user to customize the
features of the spectrophotometer.
DARK CURRENT
Dark Current is a maintenance feature and should only be used by service personnel.
It is used to refresh the system background. If the working conditions change, or the
instrument has been in use for an extended length of time, refreshing the dark current will
improve the stability.
SMART Spectro 2 Operator’s Manual 04.16 25
www.GlobalTestSupply.com
Page 26
CALIBRATE WL
The Calibrate Wavelength (CALIBRATE WL) mode is used to establish or re-establish
the accuracy of the wavelength selection process. Normally, the Calibrate Wavelength
procedure should be run after the SMART Spectro 2 is turned ON and allowed to warm
up for 15 minutes or if operating conditions (temperature, humidity, etc.) change
signifi cantly.
For fi eld use, when operating with the battery, calibrate wavelength prior to going into
the fi eld using AC power. This will increase battery life in the fi eld. Alternatively calibrate
wavelength in the fi eld immediately before testing. Turn Spectro 2 on immediately before
scanning blank. Calibrate wavelength just before scanning blank.
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
2. Press or to scroll to SYSTEM
SETUP.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
26 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 27
3. Press *ENTER to select SYSTEM SETUP. SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
4. Press or to scroll to CALIBRATE
WL.
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
5. Press *ENTER to select CALIBRATE WL. CALIBRATE WL
CALIBRATE WL???
PLEASE SELECT NO
SMART Spectro 2 Operator’s Manual 04.16 27
www.GlobalTestSupply.com
Page 28
6. Press or to highlight NO or YES.
CALIBRATE WL
CALIBRATE WL???
PLEASE SELECT NO
7. Press *ENTER to begin the wavelength
calibration.
During the calibration, the Spectro will display progress updates on the top line of the
display. The microprocessor will move the grating in search of the position that gives a
very specifi c maximum light intensity. The microprocessor will then move the grating a
precise predetermined amount from this position. This precise movement will cause the
grating to be positioned at 546 nm every time. Once calibrated the wavelength displayed
during testing is accurate to ±2 nm. When the wavelength calibration is complete the
display will go back to the System Setup Menu.
CALIBRATE WL
CALIBRATE WL???
PLEASE SELECT YES
BT PRINTER
Bluetooth wireless technology allows communication between the spectrophotometer
and the Mobile Bluetooth Printer (Code 5-0066 only). The Bluetooth feature is always on.
28 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 29
1. Turn on Mobile Bluetooth Printer (Code
5-0066).
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
2. Press ON to turn on the SMART Spectro 2.
The LaMotte logo screen will appear for
about 2 seconds. After initialization the
MAIN MENU will appear.
3. Press or to scroll to SYSTEM
SETUP.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
SMART Spectro 2 Operator’s Manual 04.16 29
www.GlobalTestSupply.com
Page 30
4. Press *ENTER to select SYSTEM SETUP. SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
5. Press or to scroll to BT PRINTER.
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
6. Press *ENTER to select BT PRINTER. BT PRINTER
CONNECT
DISCONNECT
SCAN FOR DEVICES
30 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 31
7. FIRST TIME PAIRING OF PRINTER AND SPECTRO
To pair the Mobile Spin Printer to the SMART Spectro 2 for the fi rst time, scroll to
SCAN FOR DEVICES. If the printer and SMART Spectro 2 have already been paired,
go to page 32.
Press *ENTER to select SCAN FOR
DEVICES.
Press *ENTER to select scan for devices.
The spectro will search and create a list of
available Bluetooth devices.
BT PRINTER
CONNECT
DISCONNECT
SCAN FOR DEVICES
SCAN DEVICES
1 000D163140A0, 0M2100-0
2 9CD21EE77F08, USER-PC
3 00066667B737, WLSAPPLE
Scroll to the device ending in
OM2100-0. This is the PIN code of a Mobile
Bluetooth Printer (Example,
1. 000D163140A0, OM2100-0).
SCAN DEVICES
1 000D163140A0, 0M2100-0
2 9CD21EE77F08, USER-PC
3 00066667B737, WLSAPPLE
Press *ENTER select the Mobile Bluetooth
Printer.
If the connection to the Mobile Bluetooth Printer is successful, CONNECTED!!! and a printer
icon
will appear on the top line of the display. The printer icon will remain as long as
the printer is connected.
SMART Spectro 2 Operator’s Manual 04.16 31
www.GlobalTestSupply.com
Page 32
7. PRINTER AND SPECTRO ALREADY PAIRED
If the printer and spectro have already been paired, scroll to CONNECT.
Press *ENTER to select CONNECT. BT PRINTER
CONNECT
DISCONNECT
SCAN FOR DEVICES
If the connection to the Mobile Bluetooth Printer is successful, CONNECTED!!! and a printer
icon
will appear on the top line of the display. The printer icon will remain as long as
the printer is connected.
8. Press EXIT to return to SYSTEM SETUP.
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
32 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 33
EDIT CLOCK
The clock information is used to time stamp the data points in the data logger.
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
2. Press or to scroll to
SYSTEM SETUP.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
3. Press *ENTER to select SYSTEM SETUP. SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
SMART Spectro 2 Operator’s Manual 04.16 33
www.GlobalTestSupply.com
Page 34
4. Press or to scroll to
EDIT CLOCK.
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
5. Press *ENTER to select EDIT CLOCK. The
display will show seconds-minuteshours-day-month-year.
Note: Hours are in a 24 hour format.
6. Press or to scroll to adjust the
seconds to the correct time.
EDIT CLOCK
00:13:10 14-04-2016
SET SECOND: 44
EDIT CLOCK
00:13:10 14-04-2016
SET SECOND: 46
34 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 35
7. Press *ENTER to save the second setting. EDIT CLOCK
46:13:10 14-04-2016
SET SECOND: 15
8. Follow the procedure to set the minutes,
hour, month, day, and year. Press EXIT to
return to the MAIN MENU.
STORE METHOD
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
SMART Spectro 2 Operator’s Manual 04.16 35
www.GlobalTestSupply.com
Page 36
2. Press or to scroll to
SYSTEM SETUP.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
3. Press *ENTER to select SYSTEM SETUP. SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
4. Press or to scroll to
STORE METHOD.
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
36 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 37
5. Press *ENTER to select STORE METHOD. STORE METHOD
AUTOMATIC
MANUAL
END OF LIST
6. Press or to select the desired
store method. In the AUTO method, all
results will automatically be stored in the
data logger. This is the default setting.
In the MANUAL method, the user will
be prompted after each test to decide
whether to store the result.
Choose the MANUAL method to avoid
saving unecessary data and using up
space in the memory.
7. Press *ENTER to select the store method
and return to the SYSTEM SETUP MENU.
STORE METHOD
AUTOMATIC
MANUAL
END OF LIST
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
SMART Spectro 2 Operator’s Manual 04.16 37
www.GlobalTestSupply.com
Page 38
BEEPER ON/OFF
If the beeper function is turned ON, the beeper will sound at each button push. The default
beeper setting is ON.
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
2. Press or to scroll to
SYSTEM SETUP.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
3. Press *ENTER to select SYSTEM SETUP. SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
38 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 39
4. Press or to scroll to
BEEPER ON/OFF.
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
5. Press *ENTER to select BEEPER ON/OFF. BEEPER ON/OFF
ON
OFF
6. Press or to highligh ON or OFF.
BEEPER ON/OFF
ON
OFF
SMART Spectro 2 Operator’s Manual 04.16 39
www.GlobalTestSupply.com
Page 40
7. Press *ENTER to select ON or OFF. BEEPER ON/OFF
ON
OFF
8. Press EXIT to return to the SYSTEM SETUP. SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
CORRECTION WL
The Correction WL feature should only be used by service personnel. It is used to calibrate
the system and the wavelength.
40 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 41
LANGUAGE
There are two choices for language: English and Chinese. The default language is English.
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
2. Press or to scroll to
SYSTEM SETUP.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
3. Press *ENTER to select SYSTEM SETUP. SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
SMART Spectro 2 Operator’s Manual 04.16 41
www.GlobalTestSupply.com
Page 42
4. Press or to scroll to
LANGUAGE.
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
5. Press *ENTER to select LANGUAGE. LANGUAGE
ENGLISH
CHINESE
6. Press or to scroll to highlight
ENGLISH or CHINESE.
LANGUAGE
ENGLISH
CHINESE
42 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 43
7. Press *ENTER to save the selection and
return to the SYSTEM SETUP.
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
RESET TO DEFAULT
The SMART Spectro 2 can be reset to the default settings.
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
2. Press or to scroll to
SYSTEM SETUP.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
SMART Spectro 2 Operator’s Manual 04.16 43
www.GlobalTestSupply.com
Page 44
3. Press *ENTER to select SYSTEM SETUP. SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
4. Press or to scroll to
END OF LIST.
5. Press and hold *ENTER for 8 seconds to
reset the system to Default. Progress bars
will show the reset progress of the system
data (SD) and the customer data (UD)
SYSTEM SETUP
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
END OF LIST
MAIN MENU
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
44 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 45
6. When the default reset is complete the
display will return to the SYSTEM SETUP
Menu.
SYSTEM SETUP
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
END OF LIST
INITIALIZATION
When ON is pressed to turn the spectrophometer on the meter will automatically go
through initialization and begin a 15 minute warm up. To skip the 15 minute warm up,
press EXIT. After initialization, the main menu will be displayed.
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
SMART Spectro 2 Operator’s Manual 04.16 45
www.GlobalTestSupply.com
Page 46
PROGRAMMED TESTS
INTRODUCTION
The PROGRAMMED TESTS mode is used to run all LaMotte pre-programmed tests and
USER TESTS. This is also where USER TESTS and SEQUENCES are set-up and edited.
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
2. Press or to scroll to
PROGRAMMED TESTS.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
3. Press *ENTER to select PROGRAMED
TESTS. In the PROGRAMMED TESTS menu
there are three alterable sequences and
one ALL TESTS fi xed sequence as well as
the EDIT function.
46 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
PROGRAMMED TESTS
SEQUENCE 1
SEQUENCE 2
SEQUENCE 3
ALL TESTS
EDIT
Page 47
SEQUENCES OF TESTS
SEQUENCE 1, SEQUENCE 2, and SEQUENCE 3 are alterable sequences. They may be edited
using the EDIT function mode. Any of the LaMotte pre-programmed tests or User Tests
may be placed in these sequences in whatever testing order that is preferred. Some
examples of typical sequences are given below.
SEQUENCE 1 SEQUENCE 2 SEQUENCE 3
60 Molybdenum-LR 1 Alkalinity-UDV 3 Ammonia-N L F
79 Phosphate H 35 Cyanide 32 Copper DDC
9 Bromine-LR 41 Fluoride 64 Nitrate-N LR
76 pH TB 53 Iron Phen 67 Nitrite-N LR
15 Chlorine 55 Manganese L 74 pH CPR
86 Silica HI 64 Nitrate-N LR 78 Phosphate L
45 Hydrazine 26 COD SR 0-1500 85 Silica Lo
32 Copper DDC 77 Phenols END OF LIST
51 Iron Bipyr 78 Phosphate L
END OF LIST 90 Sulfi de-LR
END OF LIST
NOTE: Sequences always end with END OF LIST to indicate that there are no more tests in
the sequence.
These alterable sequences allow a series of tests to be setup that are run frequently. The
order of the individual tests in the sequence is determined by the user. After running a
test, press EXIT to escape back to the Sequence menu. Move the
test listed and press *ENTER. Continue this pattern until the entire sequence has been
completed.
ALL TESTS is a fi xed sequence containing the LaMotte pre-programmed tests and User
Tests.
Modifi cation of the alterable sequence is accomplished through the EDIT function. This
function is explained in detail in the section titled EDIT.
It should be noted that if a %T/ABS test is to be included in a sequence, the
%T/ABS test must fi rst be setup as a User Test (but no actual calibration needs to be
performed, only select a name and wavelength).
Pressing the EXIT button while in a sequence menu will escape back to the PROGRAMMED
TESTS menu.
Pressing and holding the OFF button at any time will turn the SMART Spectro 2 off .
SMART Spectro 2 Operator’s Manual 04.16 47
www.GlobalTestSupply.com
down to the next
Page 48
GENERAL TESTING PROCEDURES
The following are some step by step examples of how to run tests from the PROGRAMMED
TESTS menu. These test procedures are designed to be used with LaMotte SMART Spectro
2 reagent systems.
TESTING WITH THE PROGRAMMED TESTS
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
2. Press or to scroll to
PROGRAMMED TESTS.
3. Press *ENTER to select PROGRAMED
TESTS.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
PROGRAMMED TESTS
SEQUENCE 1
SEQUENCE 2
SEQUENCE 3
ALL TESTS
EDIT
48 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 49
4. Press or to scroll to
ALL TESTS.
PROGRAMMED TESTS
SEQUENCE 1
SEQUENCE 2
SEQUENCE 3
ALL TESTS
EDIT
5. Press *ENTER to select ALL TESTS. ALL TESTS
1 ALKALINITY-UDV
2 ALUMINUM
3 AMMONIA-N L F
4 AMMONIA-N L S
5 AMMONIA-N H
6 ARSENIC
7 BIGUANIDE
8 BORON
6. Press or to scroll to the desired
test.
ALL TESTS
1 ALKALINITY-UDV
2 ALUMINUM
3 AMMONIA-N L F
4 AMMONIA-N L S
5 AMMONIA-N H
6 ARSENIC
7 BIGUANIDE
8 BORON
SMART Spectro 2 Operator’s Manual 04.16 49
www.GlobalTestSupply.com
Page 50
7. Press *ENTER to select the test. 2 ALUMINUM 535 NM
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
8. Insert the blank. Close lid. Press *ENTER
to scan the blank. BLANKING will
momentarily appear on the top line of the
display. The blank has been stored.
9. Insert the reacted sample. Close lid. Press
*ENTER to scan the sample. The result will
be displayed.
If Store Method is automatic, STORE
PRESS [ENTER] will not be displayed.
2 ALUMINUM 535 NM
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
2 ALUMINUM 535 NM
T = 13.5%T
A = 0.8707A
C = 0.26 ppm
STORE PRESS [ENTER]
50 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 51
10. Press *ENTER to store the result to
the Test Resultl List. If Store method is
automatic, the test result will be stored
automatically. To repeat the test, press
EXIT to escape the test screen, then press
*ENTER to scan the sample again. The
last blank scanned is used to zero the
spectrophotometer for repeat scans. A
diff erent blank can be used by pressing
the button to scroll back to SCAN BLANK
and then scanning another blank.
To print the result, scroll to the Test Result
List. Press *ENTER. Press
move the * next to the desired data. Press
*ENTER.
or to
SETUP & EDIT SEQUENCES & USER TESTS
The EDIT menu, found in the PROGRAMMED TESTS Menu, allows any of the three alterable
test sequences (SEQUENCE 1, SEQUENCE 2, and SEQUENCE 3) and any of the 25 User
Tests in the ALL TESTS fi xed sequence to be edited. This feature allows a sequence or test
which is used frequently to be set-up for easy access. The order of the sequence can be
arranged to suit the needs of the user. Any combination, and any order of tests from ALL
TESTS (including User Tests), may be placed into these sequences.
EDIT A SEQUENCE
1. Go to the PROGRAMMED TESTS menu.
Press or to scroll to EDIT.
SMART Spectro 2 Operator’s Manual 04.16 51
www.GlobalTestSupply.com
PROGRAMMED TESTS 535NM
SEQUENCE 1
SEQUENCE 2
SEQUENCE 3
ALL TESTS
EDIT
Page 52
2. Press *ENTER to select EDIT. EDIT 535NM
EDIT SEQUENCE 1
EDIT SEQUENCE 2
EDIT SEQUENCE 3
EDIT USER TESTS
EDIT
ADDING OR DELETING TESTS
There are two ways to alter a sequence - INSERT and DELETE.
INSERT is used to add a new test to a sequence and to place the new test before an
existing test in a sequence.
DELETE is used to remove an existing test from a sequence.
Below is a step by step example of how to ADD a test to SEQUENCE 3 starting from the
EDIT menu.
1. Press to scroll to the sequence to be
edited.
52 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
EDIT 535NM
EDIT SEQUENCE 1
EDIT SEQUENCE 2
EDIT SEQUENCE 3
EDIT USER TESTS
Page 53
2. Press *ENTER to select the sequence to
be edited.
EDIT SEQUENCE 3 535NM
3. Press *ENTER to view the list of editing
options.
4. Press *ENTER to select INSERT to insert a
test into the sequence.
EDIT SEQUENCE 3 535NM
INSERT
DELETE
INSERT
1 ALKALINITY-UDV
2 ALUMINUM
3 AMMONIA-N L F
4 AMMONIA-N L S
5 AMMONIA-N H
6 ARSENIC
7 BIGUANIDE
8 BORON
SMART Spectro 2 Operator’s Manual 04.16 53
www.GlobalTestSupply.com
Page 54
5. Press or to scroll to the desired
test.
INSERT 535NM
1 ALKALINITY-UDV
2 ALUMINUM
3 AMMONIA-N L F
4 AMMONIA-N L S
5 AMMONIA-N H
6 ARSENIC
7 BIGUANIDE
8 BORON
6. Press *ENTER to select the test. EDIT SEQUENCE 3 535NM
2 ALUMINUM
7. Press *ENTER to save the fi rst test in
the sequence and return to editing the
sequence.
EDIT SEQUENCE 3 535NM
INSERT
DELETE
2 ALUMINUM
54 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 55
8. Press *ENTER to select another test to
add to the sequence. The second test will
be added above the fi rst in the sequence.
INSERT 535NM
1 ALKALINITY-UDV
2 ALUMINUM
3 AMMONIA-N L F
4 AMMONIA-N L S
5 AMMONIA-N H
6 ARSENIC
7 BIGUANIDE
8 BORON
9. Press or to scroll to the desired
test.
10. Press *ENTER to add the second test
to the list above the fi rst test in the
sequence. SEQUENCE 3 has now been
modifi ed and will remain until it is deleted
again. To run a test in SEQUENCE 3 go to
the PROGRAMMED TESTS menu. Press
EXIT to exit the EDIT SEQUENCE 3 menu
and return to the EDIT menu. Press EXIT
again to exit the EDIT menu. The meter
will save any changes and go to the
PROGRAMED TESTS menu.
INSERT 535NM
72 Ozone HR
73 Palladium
74 pH CPR
75 pH PR
76 pH TB
77 Phenol
78 Phosphate L
79 Phosphate H
EDIT SEQUENCE 535NM
78 Phosphate L
2 Aluminum
SMART Spectro 2 Operator’s Manual 04.16 55
www.GlobalTestSupply.com
Page 56
Below is an example of how to DELETE a test from SEQUENCE 3, which was just created,
starting from the EDIT menu.
1. Press to scroll to the sequence to be
edited.
2. Press *ENTER to view the list of editing
options.
EDIT 535NM
EDIT SEQUENCE 1
EDIT SEQUENCE 2
EDIT SEQUENCE 3
EDIT USER TESTS
EDIT SEQUENCE 3 535NM
78 Phosphate L
2 Aluminum
3. Press or to scroll to the test to be
deleted.
EDIT SEQUENCE 3 535NM
78 Phosphate L
2 Aluminum
56 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 57
4. Press *ENTER to select the test to be
deleted.
EDIT SEQUENCE 3 535NM
INSERT
DELETE
2 ALUMINUM
5. Press or to scroll to DELETE.
6. Press *ENTER to select DELETE. Sequence
3 has now been modifi ed and will remain
until it is edited again. To run a test in
SEQUENCE 3 go to the PROGRAMMED
TESTS menu. Press EXIT to exit the EDIT
SEQUENCE 3 menu to the EDIT menu.
Press EXIT to exit the EDIT menu. The
meter will save any changes and go the
PROGRAMMED TESTS menu.
EDIT SEQUENCE 3 535NM
INSERT
DELETE
2 ALUMINUM
EDIT SEQUENCE 535NM
78 Phosphate L
SMART Spectro 2 Operator’s Manual 04.16 57
www.GlobalTestSupply.com
Page 58
EDIT USER TESTS
If a test other than the LaMotte programmed tests is performed regularly, a calibration for
it may be entered in one of the 25 User Tests. These tests are originally named “User Test
1 - 25”. It will be possible to rename the test, select a wavelength, enter a new calibration
and select the number of decimal places used to display the results. A User Test may
be added for a reagent system for which no precalibrated test exists. A calibration of
a LaMotte reagent system may also be entered. The calibration of a User Test can be
changed at any time.
The User Tests have the ability to handle between 1 and 8 data points. The curve fi tting
options of linear least squares or linear least squares through zero are available. This
requires that the test have a linear calibration, if accurate results are expected. The
spectrophotometer will determine the Absorbance of the standards and calculate a
response that will be stored to determine the concentration of future samples of unknown
concentration. These standards should cover all the concentrations for the range of
the test being performed and be scanned beginning with the lowest concentration
and fi nishing with the highest concentration (for more information about this, see
CALIBRATION CURVES, page 13). Prepare these solutions prior to entering a new
calibration.
NOTE: A calibration procedure must be performed before using any of the User Tests. If a
User Test is selected before a calibration has been entered the message “not yet available
press exit please” will appear.
The User Tests can be placed in any of the alterable sequences using the EDIT mode.
1. Go to PROGRAMMED TESTS menu. Press
to scroll to EDIT.
58 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
PROGRAMMED TESTS 535NM
SEQUENCE 1
SEQUENCE 2
SEQUENCE 3
ALL TESTS
EDIT
Page 59
2. Press *ENTER to select EDIT. EDIT 535NM
EDIT SEQUENCE 1
EDIT SEQUENCE 2
EDIT SEQUENCE 3
EDIT USER TESTS
3. Press to scroll to EDIT USER TESTS.
EDIT 535NM
EDIT SEQUENCE 1
EDIT SEQUENCE 2
EDIT SEQUENCE 3
EDIT USER TESTS
4. Press *ENTER to select EDIT USER TESTS. EDIT USER TEST 535NM
101 USER TEST 1
102 USER TEST 2
103 USER TEST 3
104 USER TEST 4
105 USER TEST 5
106 USER TEST 6
107 USER TEST 7
108 USER TEST 8
SMART Spectro 2 Operator’s Manual 04.16 59
www.GlobalTestSupply.com
Page 60
5. Press or to scroll to the user test to
be edited.
EDIT USER TEST 535NM
101 USER TEST 1
102 USER TEST 2
103 USER TEST 3
104 USER TEST 4
105 USER TEST 5
106 USER TEST 6
107 USER TEST 7
108 USER TEST 8
6. Press *ENTER to select the user test to be
edited.
NOTE: The menu allows a test to be
renamed, a wavelength to be selected,
reacted standards to be scanned and the
numerical format of the displayed test
result to be selected. After editing any
102 USER TEST 2 535NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
one of these menu choices the display
will return to this menu. Any menu choice
can be edited at any time by selecting
it. The normal procedure would be to
start with NAME THE TEST, then SELECT
WAVELENGTH, then NEW CALIBRATION,
and then FORMAT RESULT.
NAMING THE TEST
A NAME can be up to 14 characters long. The menu choices for each character are 26
letters A to Z, ten numerals 0 to 9, a space, a dash, a decimal point, and a ! as a terminator.
Selecting the terminator indicates the end of the name and stores the name. The
terminator, !, is the fi rst menu choice since it is the one character that will always have to
be selected. It is before the letter A. DO NOT CHOOSE THE ! UNTIL THE NAMING OF THE TEST
HAS BEEN COMPLETED. The terminator should be selected following the end of the name.
It must be the last character selected.
60 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 61
1. The user test to be edited has been
selected.
102 USER TEST 2 535NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
2. Press *ENTER to select NAME THE TEST. 102 USER TEST 2 535NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
NAME: 102
! A B C D E F G H I J K L M N O P Q R S
T U V W X Y Z 0 1 2 3 4 5 6 7 8 9 _ .
3. Press or to scroll to highlight
the fi rst character in the name, in this
example the test willl be named H2O.
102 USER TEST 2 535NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
NAME: 102
! A B C D E F G HI J K L M N O P Q R S
T U V W X Y Z 0 1 2 3 4 5 6 7 8 9 _ .
SMART Spectro 2 Operator’s Manual 04.16 61
www.GlobalTestSupply.com
Page 62
4. Press *ENTER to select the highlighted
character. The character will appear next
to the test number.
102 USER TEST 2 535NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
NAME: 102 H
! A B C D E F G HI J K L M N O P Q R S
T U V W X Y Z 0 1 2 3 4 5 6 7 8 9 _ .
5. Select the remaining characters in the
name one at a time, from left to right
by using the or to scroll to the
character and pressing *ENTER to select
the character. Continue in this fashion
until entire name is entered. Press EXIT
to delete/erase the entire entry and start
102 H2O 535NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
over. Select ! at the end of the name to
save the name. The display will return to
the USER TEST menu. Note that the 102
User Test is now named H2O.
SELECTING THE WAVELENGTH
1. Scroll to SELECT WL. 102 H2O 535NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
62 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 63
2. Press *ENTER to select SELECT WL. 102 H2O 535NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
PLEASE SELECT WL = 535
3. Press or to scroll to desired
wavelength.
4. Press *ENTER to save the wavelength. The
display will return to the USER TEST menu.
102 H2O 535NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
PLEASE SELECT WL = 465
102 H2O 465NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
SMART Spectro 2 Operator’s Manual 04.16 63
www.GlobalTestSupply.com
Page 64
ENTERING A NEW CALIBRATION
1. Press to scroll to NEW CALIBRATION.
2. Press *ENTER to select NEW
CALIBRATION.
102 H2O 465NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
102 H2O 465NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
INPUT DATA NUMBERS =
3. Press or to select the number of
data points that will be used to create the
calibration curve. The maximum number
of data points is 8.
64 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
102 H2O 465NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
INPUT DATA NUMBERS =
4
Page 65
4. Press *ENTER to save the selection. NEW CALIBRATION 465NM
ENTER STD. 1
ENTER STD. 2
ENTER STD. 3
ENTER STD. 4
CALCULATE
5. Press *ENTER to select the fi rst data
point.
6. Place a blank in the sample chamber.
Close lid. Press *ENTER to SCAN BLANK.
The display will indicate that the blank is
being scanned. The display will indicate
BLANKED. A highlighted box will appear on
the character selection line.
ENTER STD. 1 465NM
SCAN BLANK
STD. 1 =
! 0 1 2 3 4 5 6 7 8 9 .
ENTER STD. 1 465NM
BLANKED
STD. 1 =
! 0 1 2 3 4 5 6 7 8 9 .
SMART Spectro 2 Operator’s Manual 04.16 65
www.GlobalTestSupply.com
Page 66
7. Place the reacted standard of the lowest
concentration in the chamber. Press
or to highlight each character in the
concentration value. Press *ENTER after
each selection to confi rm the selection.
ENTER STD. 1 465NM
BLANKED
STD. 1 =
2 3 4 5 6 7 8 9 .
! 0
1
8. After the concentration has been entered,
scroll to !. Press *ENTER to save the value
and scan the standard. The absorbance
will be displayed.
9. Press EXIT to return to the New Calibration
menu.
ENTER STD. 1 465NM
BLANKED
STD. 1 = 1.0
A = 0.4116
NEW CALIBRATION 465NM
ENTER STD. 1
ENTER STD. 2
ENTER STD. 3
ENTER STD. 4
CALCULATE
66 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 67
10. Press *ENTER to move to the second
standard. Use the same procedure to
scan the blank and then scan the second
standard to create a second data point.
Repeat the procedure until the data from
all of the standards has been entered into
the calibration.
NEW CALIBRATION 465NM
ENTER STD. 1
ENTER STD. 2
ENTER STD. 3
ENTER STD. 4
CALCULATE
NOTE: To escape from NEW CALIBRATION before completing a standard selection, select
blank and press *ENTER. After blanking, scroll to ! and press *ENTER. Then press EXIT.
11. Press *ENTER to select CALCULATE. SELECT DEGREES 465NM
1. DEGREE THRU.0
1. DEGREE
2. DEGREE
3. DEGREE
12. SCROLL or to select the desired
curve fi t.
SELECT DEGREES 465NM
1. DEGREE THRU 0
1. DEGREE
2. DEGREE
3. DEGREE
Note: 1. DEGREE THRU.0 calculates the best straight line fi t through the data points and
intersects with the origin at 0 ppm, 0 absorbance. This is a classical Beers Law calibration.
If a one point calibration has been performed, the 1. DEGREE THRU.0 curve fi t must be
SMART Spectro 2 Operator’s Manual 04.16 67
www.GlobalTestSupply.com
Page 68
chosen. 1. DEGREE calculates the best straight line fi t but without forcing the line through
the origin. The minimum number of standards needed for a calibration is one for 1.
DEGREE THRU.0 and two for 1. DEGREE.
NOTE: 2. DEGREE and 3. DEGREE calculations are not available yet.
13. Press *ENTER to select the desired
curve fi t type. The display will show the
constraints for the best fi t line.
1. DEGREE 465NM
K0 = 0.6548
K1 = 5.6365
K2 = 0.0000
K3 = 0.0000
PRESS [EXIT] TO SKP...
14. Press EXIT to return to the SELECT
DEGREES menu. Press EXIT again to
return to NEW CALIBRATION. Press EXIT
again to return to the USER TEST menu.
102 H2O 465NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
SELECTING THE NUMERICAL FORMAT OF THE RESULT
The number of decimal places displayed for a result can be selected. A test which ranges
from 20 to 1000 ppm should not be displayed with three decimal places. A test with a
range from 0.010 to 0.500 needs three decimal places. The microprocessor will always
calculate the concentration to many more signifi cant fi gures than will be displayed. Menu
choices of 0, 1, 2, or 3 decimal places will be given for the display.
68 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 69
1. Press to scroll to FORMAT RESULT.
102 H2O 465NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
2. Press *ENTER to select FORMAT RESULT. DECIMAL PLACES 465NM
0 PLACES
1 PLACES
2 PLACES
3 PLACES
3. Press or to select the number of
decimal places to be displayed.
DECIMAL PLACES 465NM
0 PLACES
1 PLACES
2 PLACES
3 PLACES
SMART Spectro 2 Operator’s Manual 04.16 69
www.GlobalTestSupply.com
Page 70
4. Press *ENTER to select the number
format.
102 H2O 465NM
NAME THE TEST
SELECT WL
NEW CALIBRATION
FORMAT RESULT
5. Press EXIT to return to EDIT USER TEST
menu. Press EXIT again to escape to
EDIT menu and again to return to the
PROGRAMMED TEST menu.
Note: Test 102 was USER TEST 2 and now
is H2O. It is still a USER TEST because its
calibration can be changed but it has a
diff erent name.
EDIT USER TESTS 465NM
MEASURING IN THE %T/ABS MODE
1. Press ON. The LaMotte logo screen
will appear for about 2 seconds. After
initialization the MAIN MENU will appear.
MAIN MENU 12:00:00
101 USER TEST 1
102 H2O
103 USER TEST 3
104 USER TEST 4
105 USER TEST 5
106 USER TEST 6
107 USER TEST 7
108 USER TEST 8
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
70 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 71
2. Press or to scroll to
%T/ABS.
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
3. Press *ENTER to select %T/ABS. %T/ABS 465NM
SELECT WL
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
END OF LIST
4. Press *ENTER to select SELECT WL. %T/ABS 465NM
SELECT WL
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
END OF LIST
PLEASE SELECT WL = 465
SMART Spectro 2 Operator’s Manual 04.16 71
www.GlobalTestSupply.com
Page 72
5. Press or to scroll to desired
wavelength.
%T/ABS 465NM
SELECT WL
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
END OF LIST
PLEASE SELECT WL = 466
6. Press *ENTER to select the wavelength.
The meter is ready to scan.
7. Insert the blank into the chamber. Close
lid. Press *ENTER to scan the blank. Note:
For most %T/ABS tests, a clear, colorless
blank should be used.
%T/ABS 466NM
SELECT WL
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
END OF LIST
%T/ABS 466NM
SELECT WL
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
END OF LIST
72 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 73
8. Insert the reacted sample into the
chamber. Press *ENTER to scan the
sample. The result will be displayed.
%T/ABS 466NM
T = 79.5%T
A = 0.0996A
STORE PRESS [ENTER]
9. Press *ENTER to store the result. Press
EXIT to skip storing the result.
NOTE: If AUTOMATIC is chosen for the
STORE METHOD the test result will be
stored automatically.
At this point, it is possible to scan another
sample, scan another blank, or select
another wavelength. If no more samples
are to be scanned, press EXIT to return to
the MAIN MENU or press OFF to turn the
spectrophotometer off .
10. To view the test results, scroll to TEST
RESULT LIST.
%T/ABS 466NM
SELECT WL
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
END OF LIST
%T/ABS 466NM
SELECT WL
SCAN BLANK
SCAN SAMPLE
TEST RESULT LIST
END OF LIST
SMART Spectro 2 Operator’s Manual 04.16 73
www.GlobalTestSupply.com
Page 74
11. Press *ENTER to select TEST RESULT LIST. %T/ABS 466NM
DATE-TIME WL ABS %T
*1309:22 546 0.0000 100.0
1309:02 546 0.2763 52.9
1308:55 466 0.0996 79.5
3013:58 570 1.2363 5.8
3013:37 520 1.7479 1.8
3013:37 430 1.4883 3.2
STORE, DELETE AND PRINT DATA
STORE DATA
Data can be stored manually or automatically after taking a reading in the PROGRAMMED
TESTS mode and the %T/ABS mode. (See STORE METHOD, page 35). The information that
is saved for each reading is Date/Time, Absorbance, %T, and ppm for Programmed Tests
and Date/Time, wavelength, absorbance, and %T for %T/Abs readings. The latest test
result will be saved to the top of the list. Use up arrow or down arrow to scroll through the
data.
The data storage capacity is 1000 test results for %T/ABS tests and up to 1000 test
results for each quantitative test.
Stored data can be viewed by choosing the TEST RESULT LIST option from an individual
test factor menu or the %T/ABS menu.
Stored data can be exported or printed with the Mobile Bluetooth Printer (Code 3-0066)
by selecting PC Link from the MAIN MENU. To establish a connection with the printer go to
SYSTEM SETUP and select BT PRINTER.
74 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 75
DELETE DATA
To delete all saved data:
1. Scroll to SYSTEM SETUP on the MAIN
MENU
2. Press *ENTER to select SYSTEM SETUP. SYSTEM SETUP
MAIN MENU 12:00:00
PROGRAMMED TESTS
%T/ABS
PC LINK
SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
3. Scroll to STORE METHOD. SYSTEM SETUP
DARK CURRENT
CALIBRATE WL
BT PRINTER
EDIT CLOCK
STORE METHOD
BEEPER ON/OFF
CORRECTION WL
LANGUAGE
SMART Spectro 2 Operator’s Manual 04.16 75
www.GlobalTestSupply.com
Page 76
4. Press *ENTER to select STORE METHOD. STORE METHOD
AUTOMATIC
MANUAL
END OF LIST
5. Scroll to END OF LIST. STORE METHOD
AUTOMATIC
MANUAL
END OF LIST
6. Press and hold *ENTER for 8 seconds to
clear the memory.
WARNING: This will delete all of the saved data.
76 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 77
PRINT DATA
Data can be printed directly for a PROGRAMMED TESTS scan, %T/ABS scan, or from the
Test Result List for Programmed Tests or Test Result List for %T/Abs.
If the Store Method is Automatic and the printer is disconnected, the data point will
automatically be stored and can be printed from the Test Result List once the printer has
been connected. Use the up arrow and down arrow to scroll through the data. The data
points are printed one at a time. A group of data points can not be selected.
If the Store Method is Manual and the printer is disconnected, press *ENTER to store
the data point. Data can be printed from the Test Result List once the printer has been
connected. Use the up arrow and down arrow to scroll through the data.
If the Store Method is Automatic and the printer is connected, press *ENTER to print.
If the Store Method is Manual and the printer is connected, press *ENTER to store the data
point. Then press *ENTER to print.
PC LINK
The SMART Spectro 2 may be interfaced with any Windows-based computer by using a
LaMotte SMARTLink Program and USB Cable. The program will store test information and
results in a database. To transfer data from the meter to a computer, plug the smaller
end of the USB cable (USB mini B connector) into the meter and the larger end of the
USB cable (USB Type A connector) into a USB port on a computer. The SMART Spectro
2 will send the following data: test name, wavelength, concentration, transmittance,
absorbance, sample, blank, time of test, and date of test. For more information contact
LaMotte.
SEND 25 GROUP DATA
Press ENTER to send 25 test results to a Bluetooth master device (like a Bluetooth-ready
computer). The Bluetooth master device must have an app to pull the data.
SEND ALL DATA
Press ENTER to send all the test results to a Bluetooth master device (like a Bluetoothready computer). The Bluetooth master device must have an app to pull the data.
UPDATA PROGRAMMED TESTS
This a service feature that is used by a service professional to update programmed tests.
SMART Spectro 2 Operator’s Manual 04.16 77
www.GlobalTestSupply.com
Page 78
BATTERY OPERATION
The SMART Spectro 2 can be run using battery power. The battery pack consists of a
rechargeable Ni-metal hydride battery pack. The Battery Pack (Code 2000-BP) and
Battery Charger (Code 2000-BC) are not included in the standard meter package and
must be purchased as accessories. The power cable and AC adapter also used to charge
the battery pack are included with the SMART Spectro 2.
CHARGING THE BATTERY PACK
1. Connect the Power Cable to the AC Adapter.
2. Connect the AC Adapter to the Battery Charger (2000-BC).
3. Connect the Battery Charger to the Battery Pack (2000-BP).
4. Plug the Power Cord into an outlet. The red light will illuminate. When the battery is
fully charged the green light will illuminate. A full charge will require about 5 hours.
RUNNING THE SMART SPECTRO 2 FROM THE BATTERY PACK
1. Connect the Battery Pack to the SMART Spectro 2.
CAUTION: Do not attempt connect the AC Adapter directly to the Battery Pack. The
connectors will not fi t. Do not force them. The AC Adapter must be connected to the
Battery Charger which is then connected to the Battery Pack.
MAINTENANCE
CLEANING
Clean with a damp, lint-free cloth.
DO NOT ALLOW WATER TO ENTER THE SPECTROPHOTOMETER CHAMBER OR ANY OTHER
PARTS OF THE METER.
LAMP REPLACEMENT AND REALIGNMENT
The quartz halogen bulb in the SMART Spectro 2 has an approximate life of 1000 hours. If
a test is performed and there is no response and the unit is receiving adequate power, the
light bulb may need to be re-secured or replaced. With the meter power ON check the rear
lamp access panel to see if any light acan be observed through the vents. If the lamp is not
ON it is either loose or burnt out.
78 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 79
Lamp bracket assembly mounted
on the wall with screws (2)
Lamp Bracket Assembly Installed
Lamp bracket guiding pin
(on the mounting wall)
Lamp bracket
mounting screw (2)
Lamp bracket installation
guiding hole
Back view of the
lamp bracket
Lamp Bracket Assembly (shown with lamp)
Lamp-locking
screw (2)
SMART Spectro 2 Operator’s Manual 04.16 79
www.GlobalTestSupply.com
Page 80
HALOGEN LAMP REPLACEMENT
2)
1. Open the lamp access cover on the back of the instrument.
2. Loosen the lamp bracket mounting screws (2). Caregully remove the lamp bracket off
the mounting guide pin.
Note: Do not pull too hard to avoid breaking the wire connection to the lamp socket.
Lamp bracket
mounting screw (2)
3. Loosen the lamp-locking screws (2)
to remove the old lamp from the
lamp socket.
Lamp-locking
screw (2)
4. Insert the new lamp (12V/10W) into the lamp socket and scure the lamp with the
lamp-locking screws.
80 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 81
WARNING: DO NOT overtighten the lamp-locking screws! It may cause damage to or
even break the lamp.
5. Align the lamp bracket installation guiding hole to the guiding pin on the mounting
wall. Us the screws to lock the bracket in place. Do not tighten the screws yet.
Lamp bracket installation
guiding hole
Lamp bracket
mounting screw (2)
Larger vertical
(right) slot to
allow lamp
bracket to
move up and
down for lamp
beam alignment
6. Check the lamp alignment before securely locking the lamp bracket.
If the light beam is not focused on the entrance slit. Adjust the lamp bracket to bring
the beam into focus on the slit. Tighten the bracket locking screws.
The light beam from the lamp
must be focused on the
entrance slip.
SMART Spectro 2 Operator’s Manual 04.16 81
www.GlobalTestSupply.com
Page 82
CLOCK BATTERY (RAM BATTERY)
The SMART Spectro 2 date/clock function is powered by a CR 1220 battery. This battery
should be replaced about every 8 years. If the date or clock function stops or if you receive
the message “Warning RAM Battery Low” you should replace the RAM battery as follows,
or contact the LaMotte Technical Service Department by phone at 1-800-344-3100, fax
410-778-6394 or email tech@lamotte.com
Directions for opening the SMART Spectro 2 meter housing
1. Before proceeding, unplug the Spectro AC power supply.
2. Turn the meter over and use a long neck (4” shaft) Phillips head screw driver to
unscrew all 4 screws located in the recessed cavities. Note: do not remove the four
screws that secure the rubber “feet” to the bottom of the Spectro.
3. Holding the top and bottom together, carefully turn the meter over again. The screws
will fall out of the recessed cavity.
4. Carefully lift the top cover off . It is not necessary to disconnect any cables or wires.
CAUTION: Be careful. The electronic circuits are static sensitive. DO NOT touch the
electronic components. You do not need to touch the electronic components to replace
the RAM battery.
If you will be touching the electronic components for any other reason, you must wear a
grounding strap. If such a strap is not available then take every precaution possible to
ground yourself before and during any contact with the electronic components by holding
onto a grounded metal pipe or other grounded piece of metal. If this is not possible then at
least touch a grounded piece of metal just prior to handling any components or touching
the boards. Improper grounding can allow static buildup to short the components.
LaMotte Company is not responsible for any such damages.
Replacing the RAM Battery
1. The battery is a 3V coin battery on the top circuit board, about the size of a dime. It is
a model CR1220 or BR1225. Replace the battery with the same model type.
2. Unplug the battery by popping it out of its holder. A small insulated, fl at head screw
driver may be used to help remove the old battery.
3. Plug in the new battery with positive side facing up.
Assembly of the houseing top and bottom
1. Reassemble the spectro housing. When putting the top and bottom back together
be careful about the alignment of the metal panel on the back of the Spectro (lamp
access panel). There are slots for this panel in both the top and bottom part of the
housing.
2. Secure the top to the bottom using all 4 screws.
82 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 83
METER DISPOSAL
Waste Electrical and Electronic Equipment (WEEE)
Natural resources were used in the production of this equipment. This equipment may
contain materials that are hazardous to health and the environment. To avoid harm to
the environment and natural resources, the use of appropriate take-back systems is
recommended. The crossed out wheeled bin symbol on the meter encourages you to use
these systems when disposing of this equipment.
Take-back systems will allow the materials to be reused or recycled in a way that will not
harm the environment. For more information on approved collection, reuse, and recycling
systems contact your local or regional waste administration or recycling service.
TROUBLESHOOTING GUIDE
ERROR MESSAGES
OUT OF RANGE
If the message OUT OF RANGE is displayed when scanning a sample, the sample may be
over range or under range. If the sample is over range the sample should be diluted and
tested again (see Sample Dilution Techniques & Volumetric Measurements, page16).
BATTERY (optional)
If the symbol BAT appears in the upper left corner of the display when using the battery
pack, the battery needs to be charged. The SMART Spectro 2 will turn off if the battery
power becomes too low.
ERROR 1 TROUBLE WITH FILTER
There is a problem with fi lter positioning. This could be due to a dead motor, bad
connection to the motor, or a bad positioning detector. Contact LaMotte Technical Service
for help.
ERROR 2 TROUBLE WITH SWITCH
There is a problem with the grating positioning. This could be due to a dead stepping
motor, bad connection to stepping motor, or a bad micro switch. Contact LaMotte
Technical Service for help.
ERROR 3 TROUBLE WITH LIGHT
There is a problem locating the zero order light during wavelength calibration. Contact
LaMotte Technical Service for help.
WARNING SIGNAL TOO WEAK
Not enough light is getting to the detector. Check light path for blockage. Check lamp
position. Try recalibrating wavelength.
SMART Spectro 2 Operator’s Manual 04.16 83
www.GlobalTestSupply.com
Page 84
WARNING SIGNAL TOO STRONG
This can be an indication that the meter was accidently shut-off during wavelength
calibration. Shut meter off and restart.
WARNING RAM BATTERY LOW
Clock battery needs to be replaced soon. If it fails all user tests and Data log Data will be
lost. Unit can be run without this battery. Just use line power and leave unit on.
HELPFUL HINTS
POWER
The power supply has an internal switch that handles both 110V and 220V input.
STRAY LIGHT
The SMART Spectro 2 should have no problems with stray light. Make sure that the sample
compartment lid is always fully closed.
ACCIDENTAL LOSS OF POWER
If for any reason the meter experiences a loss of power during wavelength calibration,
the next time the meter is powered up a wavelength calibration will automatically be
performed.
DEFAULT SETTINGS
To reset the SMART Spectro 2 to the default settings, see RESET TO DEFAULT (page 43).
84 SMART Spectro 2 Operator’s Manual 04.16
www.GlobalTestSupply.com
Page 85
2
TEST
PROCEDURES
www.GlobalTestSupply.com
2000-02-MN
05.02.16
Page 86
SPECTROPHOTOMETER REAGENT SYSTEMS LIST
Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the USA)
or email at tech@lamotte.com for a current list of available calibrations and downloading
instructions.
Test Factor (Test #) Range (ppm) MDL Test Method (# of Reagents)
Alkalinity-UDV (2) 0–200 15 Unit Dose Vials (1)† 100
Aluminum (1) 0.00–0.30 0.01 Eriochrome Cyanine R (4) 50
Ammonia NitrogenLow Range, Fresh Water (3)
Ammonia NitrogenLow Range, Salt Water (4)
Ammonia NitrogenHigh Range (5)
Biguanide (7) 0–70 5 Colorimetric (1) 50
Boron (8) 0.00-0.80 0.05 Azomethine-H (2) 25
Bromine-Low Range (010) 0.00–9.00 0.04 DPD (3) 100
Bromine-UDV (11) 0.0–22.0 0.3 DPD (1)† 100
Cadmium (12) 0.00–1.00 0.02 PAN (4) 50
Carbohydrazide (013)
See Oxygen Scavengers
Calcium & Magnesium (Total),
Hardness-UDV
Chloride-TesTab (21) 0.0–30.0 0.5 Argentometric (1) 50
Chlorine-Tablet DPD (014) 0.00–4.00 0.02 DPD (3) 100
Chlorine-Free-UDV (16) 0.00–10.00 0.10 DPD (1)† 100
Chlorine-Total-UDV (18) 0.00–10.00 0.10 DPD (1)† 100
Chlorine-Liquid DPD (17) 0.00–4.00 0.30 DPD (3) 144
Chlorine Dioxide (019) 0.00–7.00 0.04 DPD (2) 100
Chromium, Hexavalent (22) 0.00–1.00 0.01 Diphenylcarbohydrazide (1) 50
Chromium, Hex, Tri, Total (22) 0.00–1.00 0.01 Diphenylcarbohydrazide (5) 50
Cobalt (24) 0.00–2.00 0.02 PAN (3) 50
COD-Low Range (25) 0–150 5 Digestion (1)* 25
COD-Standard Range (26) 0-1500 50 Digestion (1)* 25
COD-High Range (27) 0–15,000 500 Digestion (1)* 25
Color (28) 0–1,000 15 Platinum Cobalt (0) –
Copper-BCA-Low Range (29) 0.00–3.50 0.05 Bicinchoninic Acid (1) 50
Copper-Cuprizone (31) 0.00–2.00 0.01 Cuprizone (2) 50
0.00–1.00 0.02 Salicylate (3) 25
0.00–1.00 0.10 Salicylate (3) 25
0.00–4.00 0.05 Nesslerization (2) 50
0.000–0.900 0.005 Iron Reduction (3) 100
10-500 10 Unit Dose Vial (1)† 100
# of
Tests
†Requires UDV Accessory Package 1961 or 1962 (sold separately)
*Requires COD Heater Block 5-0086 (sold separately)
www.GlobalTestSupply.com
Page 87
Copper-DDC (32) 0.00–6.00 0.05 Diethyldithiocarbamate (1) 50
Copper-UDV (33) 0.00–4.00 0.20 Bicinchoninic Acid (1)† 100
Cyanide (35) 0.00-0.50 0.50 Pyridine-Barbituric Acid (5) 50
Cyanuric Acid (36) 0–200 16 Melamine (1) 40
Cyanuric Acid-UDV (37) 0–150 5 Melamine (1)† 100
DEHA (037)
0.000–0.700 0.005 Iron Reduction (3) 100
See Oxygen Scavengers
Dissolved Oxygen (39) 0.0–12.0 0.25 Winkler Colorimetric (3) 100
Erythorbic Acid (049)
0.00–3.00 0.02 Iron Reduction (3) 100
See Oxygen Scavengers
Fluoride (41) 0.0–2.0 0.1 SPADNS (2) 50
Hardness (Total) UDV (043) 10–500 10 Unit dose Vial (1)† 100
Hydrazine (45) 0.000–0.750 0.010 P-dimethyl-
50
aminobenzaldehyde (2)
Hydrogen Peroxide-
0.00–1.50 0.02 DPD (2) 100
Low Range (46)
Hydrogen Peroxide-
0–60 1 DPD (2) 100
High Range (47)
Hydrogen Peroxide-Shock (48) 0–225 4 DPD (2) 100
Hydroquinone (049)
0.00–1.80 0.01 Iron Reduction (3) 100
See Oxygen Scavengers
Iodine (050) 0.00–14.00 0.08 DPD (2) 100
Iron-Bipyridyl (51) 0.00–6.00 0.06 Bipyridyl (2)† 50
Iron-Phenanthroline (53) 0.00–4.50 0.04 1,10 Phenanthroline (2) 50
Iron-UDV (52) 0.00–10.00 0.07 Bipyridyl (1)† 100
Lead (54) 0.0–5.0 0.1 PAR (5) 50
Manganese-Low Range (55) 0.00–0.70 0.02 PAN (3) 50
Manganese-High Range (56) 0.0–15.0 0.3 Periodate (2) 50
Mercury (57) 0.00–1.50 0.02 TMK (3) 50
Methylethylketoxime (058)
0.00–3.00 0.02 Iron Reduction (3) 100
See Oxygen Scavengers
Molybdenum-High Range (61) 0.0–15.0 0.2 Thioglycolate (3) 50
Nickel (63) 0.00–8.00 0.06 Dimethylglyoxime (6) 50
Nitrate-TesTab (66) 0-60 3 Zinc Reucion (1) 50
Nitrate Nitrogen-
0.00–3.00 0.05 Cadmium Reduction (2) 20
Low Range (64)
Nitrite Nitrogen-
0.00–0.80 0.02 Diazotization (2) 20
Low Range (67)
†Requires UDV Accessory Package 1961 or 1962 (sold separately)
*Requires COD Heater Block 5-0086 (sold separately)
www.GlobalTestSupply.com
Page 88
Nitrogen, Total (62) 0-25 mg/L 2 mg/L Chromotropic Acid/Digestion
25
(6)*
Oxygen Scanvengers various various Oxygen Reduction (3) 50
Ozone-Low Range (071) 0.00–0.40 0.02 Indigo Trisulfonate (3) 100
Ozone-High Range (072) 0.00–1.50 0.05 Indigo Trisulfonate (3) 20
pH-Chlorophenol Red (073) 5.0-7.0 – Chlorophenol Red (3) 100
pH-Phenol Red (074) 6.6–8.4 – Phenol Red (3) 100
pH-Thymol Blue (075) 8.0–9.5 – Thymol Blue (3) 100
Phenol (77) 0.00-6.00 0.05 Aminoantipyrine (3) 50
Phosphate-Low Range (78) 0.00–3.00 0.04 Ascorbic Acid Reduction (2) 50
Phosphate-High Range (79) 0.0–70.0 1.0 Vanodomolybd-
50
phosphoric Acid (1)
Phosphorus, Total, Low Range
(82)
Phosphorus,
Total, High-Range (83)
0.00–3.00
mg/L
0.0–70.0
mg/L
0.07 Ascorbic Acid/Digestion* 25
5.0 mg/L Molybdovanadate/Digestion
25
(5)*
Potassium (81) 0.0-10.0 0.5 Tetraphenolboron (2) 100
Silica-Low Range (85) 0.00–2.50 0.03 Heteropoly Blue (4) 100
Silica-High Range (86) 0–50 1 Silicomolybdate (3) 50
Sulfate-High Range (89) 5–100 5 Barium Chloride (1) 100
Sulfi de-Low Range (90) 0.00–1.00 0.02 Methylene Blue (3) 50
Surfactants (94) 0.0-8.0 0.5 Bromphenol Blue (3) 50
Tannin (96) 0.0–10.0 0.2 Tungsto-molybdophosphoric
50
Acid (3)
Turbidity (98) 2-400 FTU 2 FTU Absorbtimetric (0) –
Zinc-Low Range (99) 0.00–3.00 0.03 Zincon (6) 50
†Requires UDV Accessory Package 1961 or 1962 (sold separately)
*Requires COD Heater Block 5-0086 (sold separately)
www.GlobalTestSupply.com
Page 89
ALKALINITY, UDV
UNIT DOSE VIAL METHOD • CODE 4318-J
QUANTITY CONTENTS CODE
1 Alkalinity Unit Dose Vials, 20 pouches 4318-J
Equipment needed but not supplied:
STANDARD ACCESSORY PACKAGE • CODE 1961
1 Package of 3 Vials (empty) 0156
1 Syringe, 6 mL, plastic 1184
1 Foil Storage Bag 9467
Or:
ADVANCED ACCESSORY PACKAGE • CODE 1962
1 Pipettor, 3 mL, w/tip 30529-BRAND
1 Cuvette Rack 31695
1 Package of 3 Vials (empty) 0156
1 Foil Storage Bag 9467
*WARNING: Reagents marked with an * are considered to be potential health hazards. To
view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the contents
list or in the test procedures. Omit any letter that follows or precedes the four digit code
number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy,
contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
Alkalinity is a measure of the acid-neutralizing capacity of water that enables it to resist
abrupt changes in pH. It is the sum of all titratable bases. Alkalinity is signifi cant in
maintaining proper pH levels in natural water; water used for irrigation, swimming pools,
industrial processes and wastewater treatment processes.
Test Procedures
The presence of buff ering materials in natural waters helps to neutralize acids as they
are added to, or created in, the water ecosystem. A Total Alkalinity of 100 to 200 ppm will
stabilize the pH level in a stream. In swimming pools, total alkalinity is commonly known
as a pH stabilizer because, when the alkalinity is at a proper level, a consistent pH level can
be maintained while treatment chemicals or fresh make-up water is added. In industrial
situations, alkalinity is an important factor in preventing fl uctuating pH levels that can
damage equipment and corrode pipes.
SMART Spectro 2 Test Procedures 05.02.16 ALKALINITY, UDV
www.GlobalTestSupply.com
Page 90
APPLICATION: Drinking and surface water and swimming pool water
RANGE: 0–200 ppm as CaCO3
MDL: 15 ppm
METHOD: The sample is added to a buff ered indicator reagent. The color
that develops, ranging from yellow to blue, will indicate the
amount of alkalinity in the sample.
SAMPLE HANDLING &
PRESERVATION:
Samples should be analyzed as soon as possible after
collection. Sample may be refrigerated for 24 hours.
INTERFERENCES: Quats and poly quats at high concentrations will interfere.
Test Procedures
ALKALINITY, UDV SMART Spectro 2 Test Procedures 05.02.16
www.GlobalTestSupply.com
Page 91
PROCEDURE
1. Use 10 mm square cell adapter
2. Press ON button to turn spectrophotometer on.
3. Scroll to and select PROGRAMMED TESTS.
4. Scroll to and select ALL TESTS (or another sequence containing
1 Alkalinity-UDV) from TESTING MENU.
5. Scroll to and select 1 Alkalinity-UDV from menu.
6. Rinse a clean vial (0156) with sample water.
7. Use the syringe (1184) to add 3 mL of sample to the vial.
8. Insert the vial into chamber, close lid and select SCAN BLANK.
9. Remove vial from Spectro.
10. Use the syringe (1184) to add 3 mL of sample to a Alk UDV vial (4318).
11. Wait 2 minutes.
12. Invert vial 3 times to mix.
NOTE: If powder residue remains in the bottom of the vial after inverting or air bubbles
form, invert once more and tap bottom of vial sharply once or twice to dislodge powder
and bubbles. Mix.
13. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result.
14. Press and hold OFF button to turn the spectrophotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
NOTES: For best possible results, a reagent blank should be determined to account for
any contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Then follow
the above procedure to perform the test on a distilled or deionized water sample. This test
result is the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only when a new lot
number of reagents is obtained.
Test Procedures
UDVs from opened pouches should be used promptly. Store unused vials from opened
pouches in the Foil Storage Bag (9467) to extend the shelf life of the reagent. Generally,
UDVs stored in the bag should be used within 10 days if the humidity is less than 50% and
within 5 days if humidity is greater than 50%. The Foil Storage Bag contains a dessicant
pack with indicator. When the indicator in the window turns from blue to pink, the bag
should be replaced.
SMART Spectro 2 Test Procedures 05.02.16 ALKALINITY, UDV
www.GlobalTestSupply.com
Page 92
Test Procedures
ALKALINITY, UDV SMART Spectro 2 Test Procedures 05.02.16
www.GlobalTestSupply.com
Page 93
ALUMINUM
ERIOCHROME CYANINE R METHOD • CODE 364I-01-SC
QUANTITY CONTENTS CODE
5 g * Aluminum Inhibitor Reagent *7865-C
2 x 120 mL * Aluminum Buff er Reagent *7866-J
120 mL Aluminum Indicator Reagent 7867-J
15 mL Aluminum Complexing Reagent 7868-E
1 Spoon, 0.05 g, plastic 0696
2 Pipets, 1.0 mL, plastic 0354
1 Test Tube, glass, 5 mL w/cap 0230
*WARNING: Reagents marked with an * are considered to be potential health hazards. To
view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the contents
list or in the test procedures. Omit any letter that follows or precedes the four digit code
number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy,
contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
Aluminum is the third most common element in the earth’s crust, which accounts for
its wide appearance in many water supplies. Aluminum exists in water as soluble salts,
colloidal compounds, and insoluble compounds. In wastewater that has been treated by
alum coagulation it will appear in one or more of the above forms. Properly treated drinking
water should have an aluminum concentration below 0.05 mg/L.
Test Procedures
APPLICATION: Drinking, surface, and saline waters; domestic and industrial
wastewater.
RANGE: 0.00–0.30 ppm Aluminum
MDL: 0.01 ppm
METHOD: Aluminum ions buff ered to a pH of 6.0 react with Eriochrome
Cyanine R dye to produce a pink to red complex in proportion
to the concentration.
SAMPLE HANDLING &
PRESERVATION:
INTERFERENCES: Fluoride and polyphosphate will interfere. Interference from
SMART Spectro 2 Test Procedures 05.02.16 ALUMINUM
Collect sample in acid washed glass or plastic bottle. Analyze
as soon as possible.
iron and manganese is eliminated by the addition of an
inhibitor.
www.GlobalTestSupply.com
Page 94
PROCEDURE
Use universal sample holder.
1. Press ON button to turn spectrophotometer on.
2. Scroll to and select PROGRAMMED TESTS.
3. Scroll to and select ALL TESTS (or another sequence containing
2 Aluminum).
4. Scroll to and select 2 Aluminum from menu.
5. Rinse a clean Spectro tube (0290) with sample water. Fill to the 10 mL line with
sample.
6. Insert tube into Spectro chamber and select SCAN BLANK.
7. Rinse a clean test tube (0230) with sample water. Fill to the 5 mL line with sample.
Test Procedures
8. Remove tube from Spectro. Empty sample from Spectro tube (0290).
9. Add 5 mL sample from test tube (0230) to empty Spectro tube (0290).
10. Use the 0.05 g spoon (0696) to add one measure of *Aluminum Inhibitor Reagent
(7865). Cap and mix to dissolve powder.
11. Use a 1.0 mL pipet (0354) to add 2 mL of *Aluminum Buff er Reagent (7866). Cap and
mix.
12. Use a second 1.0 mL pipet (0354) to add 1 mL of Aluminum Indicator Reagent (7867).
Cap and mix contents. Wait 5 minutes for maximum color development.
13. At end of 5 minute waiting period, mix, insert tube into chamber, close lid and select
SCAN SAMPLE. Record result.
14. Press and hold OFF button to turn the spectrophotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
NOTE: For the best possible results, a reagent blank should be determined to account for
any contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Add 5 drops of
Aluminum Complexing Reagent (7868). Then follow the above procedure to perform the
test on a distilled or deionized water sample. This test result is the reagent blank. Subtract
the reagent blank from all subsequent test results of unknown samples. It is necessary to
determine the reagent blank only when a new lot number of reagents is obtained.
ALUMINUM SMART Spectro 2 Test Procedures 05.02.16
www.GlobalTestSupply.com
Page 95
AMMONIA-NITROGEN, LOW RANGE
SALICYLATE METHOD • CODE 3659-01-SC
QUANTITY CONTENTS CODE
60 mL *Salicylate Ammonia #1 *3978-H
10 g *Salicylate #2 *7457-D
2 x 5 g *Salicylate #3 Reagent Powder *7458-C
1 Spoon, 0.1 g, plastic 0699
1 Spoon, 0.15 g, plastic 0727
1 Pipet, 1.0 mL, plastic 0354
*WARNING: Reagents marked with an * are considered to be potential health hazards. To
view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the contents
list or in the test procedures. Omit any letter that follows or precedes the four digit code
number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy,
contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
Ammonia nitrogen is present in various concentrations in many surface and ground water
supplies. Any sudden change in the concentration of ammonia nitrogen in a water supply is
cause for suspicion. A product of microbiological activity, ammonia nitrogen is sometimes
accepted as chemical evidence of pollution when encountered in natural waters.
Test Procedures
Ammonia is rapidly oxidized in natural water systems by special bacterial groups that
produce nitrite and nitrate. This oxidation requires that dissolved oxygen be available in the
water. Ammonia is an additional source of nitrogen as a nutrient which may contribute to
the expanded growth of undesirable algae and other forms of plant growth that overload
the natural system and cause pollution.
SMART Spectro 2 Test Procedures 05.02.16 AMMONIA-NITROGEN, Low Range
www.GlobalTestSupply.com
Page 96
APPLICATION: Low concentrations of ammonia in fresh, brackish and salt
water; fresh and salt water aquariums.
RANGE: 0.00–1.00 ppm Ammonia-Nitrogen
MDL: 0.02 ppm Fresh Waer
0.10 ppm Salt Water
METHOD: Salicylate and ammonia react at high pH in the presence of a
chlorine donor and an iron catalyst to form a blue indophenol
dye, the concentration of which is proportional to the ammonia
concentration in the sample.
SAMPLE HANDLE &
PRESERVATION:
Test Procedures
Ammonia solutions tend to be unstable and should be analyzed
immediately. Samples may be stored for 24 hours at 4°C or 28
days at –20°C.
INTERFERENCES: There are few interferences in most natural waters. High
concentrations of reducing agents, such as hydrazine, react
with the chlorine donor and can result in negative interferences.
Color and turbidity can also interfere.
AMMONIA-NITROGEN, Low Range SMART Spectro 2 Test Procedures 05.02.16
www.GlobalTestSupply.com
Page 97
PROCEDURE–FRESH WATER
Use universal sample holder.
1. Press ON button to turn spectrophotometer on.
2. Scroll to and select PROGRAMMED TESTS from menu.
3. Scroll to and select ALL TESTS (or another sequence containing 3 Ammonia-N L F)
from TESTING MENU.
4. Scroll to and select 3 Ammonia-N L F from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.
6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.)
7. Remove tube from Spectro. Use the 1.0 mL plastic pipet (0354) to add 2.0 mL of
*Salicylate Ammonia #1 (3978). Cap and mix.
8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent (7457).
Cap and mix until dissolved. Wait 1 minute.
9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two measures
of *Salicylate #3 Reagent Powder (7458). Cap and shake vigorously for at least
30 seconds and all solid has dissolved. Wait 12 minutes for maximum color
development.
10. At the end of 12 minute waiting period, mix, insert tube into chamber, close lid and
select SCAN SAMPLE. Record result.
11. Press and hold OFF button to turn spectrophotometer off or press EXIT button to exit
to a previous menu or make another menu selection.
Test Procedures
CALCULATIONS:
To express results as Unionized Ammonia (NH
):
3
ppm Unionized Ammonia (NH3) =
ppm Ammonia-Nitrogen (NH3–N) x 1.2
To express results as Ionized Ammonia (NH4):
ppm Ionized Ammonia (NH4+) =
ppm Ammonia-Nitrogen (NH3–N) x 1.3
NOTES: For the best possible results, a reagent blank should be determined to account for
any contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Then follow
the above procedure to perform the test on a distilled or deionized water sample. This test
result is the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only when a new lot
number of reagents is obtained.
SMART Spectro 2 Test Procedures 05.02.16 AMMONIA-NITROGEN, Low Range
www.GlobalTestSupply.com
Page 98
To determine the percentage of Ammonia-Nitrogen that is unionized and ionized, consult
the Appendix.
PROCEDURE–SALT WATER
Use universal sample holder.
1. Press ON button to turn spectrophotometer on.
2. Scroll to and select PROGRAMMED TESTS from menu.
3. Scroll to and select ALL TESTS (or another sequence containing
4 Ammonia-N L S) from TESTING MENU.
4. Scroll to and select 4 Ammonia-N L S from menu.
Test Procedures
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.
6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.)
7. Remove tube from Spectro. Use the 1.0 mL plastic pipet (0354) to add 2.0 mL of
*Salicylate Ammonia #1 (3978). Cap and mix.
8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent (7457).
Cap and mix until dissolved. Wait 1 minute.
9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two measures
of *Salicylate #3 Reagent Powder (7458). Cap and shake vigorously for at least
30 seconds and all solid has dissolved. Wait 20 minutes for maximum color
development.
10. At the end of 20 minute waiting period, mix, insert tube into chamber, close lid and
select SCAN SAMPLE. Record result.
11. Press and hold OFF button to turn spectrophotometer off or press EXIT button to exit
to a previous menu or make another menu selection.
AMMONIA-NITROGEN, Low Range SMART Spectro 2 Test Procedures 05.02.16
www.GlobalTestSupply.com
Page 99
CALCULATIONS:
To express results as Unionized Ammonia (NH3):
ppm Unionized Ammonia (NH3) =
ppm Ammonia-Nitrogen (NH3–N) x 1.2
To express results as Ionized Ammonia (NH4):
ppm Ionized Ammonia (NH
) =
4+
ppm Ammonia-Nitrogen (NH3–N) x 1.3
NOTES: For the best possible results, a reagent blank should be determined to account for
any contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Then follow
the above procedure to perform the test on a distilled or deionized water sample. This test
result is the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only when a new lot
number of reagents is obtained.
To determine the percentage of Ammonia-Nitrogen that is unionized and ionized, consult
the Appendix.
Test Procedures
SMART Spectro 2 Test Procedures 05.02.16 AMMONIA-NITROGEN, Low Range
www.GlobalTestSupply.com
Page 100
Test Procedures
AMMONIA-NITROGEN, Low Range SMART Spectro 2 Test Procedures 05.02.16
www.GlobalTestSupply.com
Loading...