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COD3 Plus
Colorimeter
Operator’s
Manual
1925-MN
Version 1.0
11.07.14
www.GlobalTestSupply.com
V2
Page 2
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CONTENTS
GENERAL INFORMATION
Packaging & Delivery ...................................................................................... 5
General Precautions ...................................................................................... 5
Safety Precautions ......................................................................................... 5
Limits of Liability ............................................................................................. 6
Warranty ......................................................................................................... 6
Register Your Meter ......................................................................................... 6
Specications ................................................................................................ 7
Statistical and Technical Denitions ............................................................... 8
Contents and Accessories ............................................................................. 9
EPA Compliance .......................................................................................... 10
CE Compliance ............................................................................................. 10
IP 67 Certication .......................................................................................... 10
CHEMICAL TESTING
Water Sampling for Chemical Analysis ........................................................... 11
Filtration ........................................................................................................... 12
An Introduction to Colorimetric Analysis ..................................................... 13
Reagent Blank ............................................................................................. 14
Colorimeter Tubes and Chamber ................................................................ 14
Meter Care .................................................................................................... 14
Selecting an Appropriate Wavelength ........................................................ 14
Calibration .................................................................................................... 15
Calibration Curves ....................................................................................... 15
Standard Additions ...................................................................................... 18
Sample Dilution & Volumetric Measurements ............................................ 19
Interferences ................................................................................................ 20
Stray Light Interference ............................................................................... 20
OPERATION OF THE COD3 PLUS COLORIMETER
Overview ...................................................................................................... 21
Components ................................................................................................ 22
GENERAL OPERATING PROCEDURES
The Keypad ................................................................................................. 23
Sample Holders ............................................................................................ 23
The Display & the Menus ............................................................................ 24
Looping Menus ............................................................................................. 26
TESTING
Testing Menu ............................................................................................... 27
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Test Sequences ........................................................................................... 28
General Testing Procedures ........................................................................ 29
Testing With LaMotte Pre-Programmed Tests ............................................. 29
Calibrating LaMotte Pre-Progammed Tests ................................................. 32
Measuring in the Absorbance Mode ............................................................ 36
EDITING MENU
Editing a Sequence ..................................................................................... 39
Adding a Test ............................................................................................... 41
Deleting a Test ............................................................................................... 43
Edit User Tests ............................................................................................. 45
Naming the Test ........................................................................................... 47
Selecting the Vial and Wavelength ............................................................. 50
Entering a Two Point Calibration ................................................................. 51
Entering a Multiple Point Calibration ............................................................. 55
Selecting the Numerical Format of the Result ............................................. 58
Selecting Units of Concentration ................................................................. 59
Setting the Clock .......................................................................................... 60
Logging Data ................................................................................................ 61
Factory Setup ............................................................................................... 62
Setting the Power Save Function ................................................................. 62
Setting the Backlight Time ............................................................................ 63
Selecting a Language ................................................................................... 64
COMPUTER CONNECTION
PC Link .......................................................................................................... 66
Output .......................................................................................................... 66
Computer Connection .................................................................................. 66
SMARTLink 3 ................................................................................................. 66
BATTERY
Battery/AC Operation .................................................................................... 66
Battery Replacement ..................................................................................... 67
MAINTENANCE
Cleaning ...................................................................................................... 68
Repairs .......................................................................................................... 68
Meter Disposal .............................................................................................. 68
TROUBLESHOOTING
Error Messages ............................................................................................ 69
Troubleshooting Guide ................................................................................. 70
COD3 Plus COLORIMETER TEST PROCEDURES
APPENDIX
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GENERAL INFORMATION
PACKAGING & DELIVERY
Experienced packaging personnel at LaMotte Company assure adequate
protection against normal hazards encountered in transportation of shipments.
After the product leaves the manufacturer, all responsibility for its safe delivery
is assured by the transportation company. Damage claims must be led
immediately with the transportation company to receive compensation for
damaged goods.
Should it be necessary to return the instrument for repair or servicing, pack
instrument carefully in a suitable container with adequate packing material.
A return authorization number must be obtained from LaMotte Company by
calling 1-800-344-3100 or emailing tech@lamotte.com. Attach a letter with the
authorization number to the shipping carton which describes the kind of trouble
experienced. This valuable information will enable the service department to
make the required repairs more efciently.
GENERAL PRECAUTIONS
Before attempting to set up or operate this instrument it is important to read the
instruction manual. Failure to do so could result in personal injury or damage to
the equipment.
The COD3 Plus Colorimeter should not be stored or used in a wet or corrosive
environment. Care should be taken to prevent water or reagent chemicals from
wet colorimeter tubes from entering the colorimeter chamber.
NEVER PUT WET TUBES IN COLORIMETER.
SAFETY PRECAUTIONS
Read the labels on all LaMotte reagent containers prior to use. Some containers
include precautionary notices and rst aid information. Certain reagents are
considered hazardous substances and are designated with a * in the instruction
manual. Material Safety Data Sheets (MSDS) can be found at www.lamotte.
com. Read the MSDS before using these reagents. Additional emergency
information for all LaMotte reagents is available 24 hours a day from the Poison
Control Center listed in the front of the phone book or by contacting the 24
hour emergency line for ChemTel 1-800-255-3924 (USA, Canada, Puerto Rico);
locations outside the North American Continent 813-248-0585 (call collect). Be
prepared to supply the name and four-digit LaMotte code number found on the
container label or at the top of the MSDS or in the contents list of the procedure.
LaMotte reagents are registered with a computerized poison control information
system available to all local poison control centers.
Keep equipment and reagent chemicals out of the reach of young children.
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LIMITS OF LIABILITY
Under no circumstances shall LaMotte Company be liable for loss of life, property,
prots, or other damages incurred through the use or misuse of its products.
WARRANTY
LaMotte Company warrants this instrument to be free of defects in parts and
workmanship for 2 years from the date of shipment. If it should become
necessary to return the instrument for service during or beyond the warranty
period, contact our Technical Service Department at 1-800-344-3100 or
tech@lamotte.com for a return authorization number or visit www.lamotte.com
for troubleshooting help. The sender is responsible for shipping charges, freight,
insurance and proper packaging to prevent damage in transit. This warranty
does not apply to defects resulting from action of the user such as misuse,
improper wiring, operation outside of specication, improper maintenance or
repair, or unauthorized modication. LaMotte Company specically disclaims any
implied warranties or merchantability or tness for a specic purpose and will not
be liable for any direct, indirect, incidental or consequential damages. LaMotte
Company’s total liability is limited to repair or replacement of the product. The
warranty set forth above is inclusive and no other warranty, whether written or
oral, is expressed or implied.
REGISTER YOUR METER
To register your meter with the LaMotte Service Department, go to
www.lamotte.com and choose SUPPORT on the top navigation bar.
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SPECIFICATIONS
INSTRUMENT TYPE: Colorimeter
Readout 160 x 100 backlit LCD, 20 x 6 line graphical display
Wavelengths 428 nm, 635 nm
Wavelength Accuracy ±2% FS
Readable Resolution Determined by reagent system
Wavelength Bandwidth 10 nm typical
Photometric Range –2 to +2 AU
Photometric Precision ± 0.001 AU at 1.0 AU
Photometric Accuracy ±0.005 AU at 1.0 AU
Sample Chamber Accepts 25 mm diameter at-bottomed test tubes, 10
mm square cuvettes, 16 mm COD test tubes
Light Sources 2 LEDs
Detectors 2 silicon photodiodes
Modes Pre-programmed tests, absorbance, %T
Pre-Programmed Tests YES, with automatic wavelength selection
User Dened Tests Up to 25 user tests can be input
Languages English, Spanish, French, Portuguese, Italian,
Chinese, Japanese
USB Port Mini B
Power Requirements USB wall adapter, USB computer connection or
lithium ion rechargeable battery
Battery Charge Life: Approximately 380 tests with backlight
on to 1000 tests with backlight off. (Signal averaging
disabled).
Battery Life: Approximately 500 charges.
Electrical Rating Provided on nameplate label
Data Logger 500 test results stored for download to a PC
Waterproof IP67 with USB port plug in place
Dimensions (LxWxH) 3.5 x 7.5 x 2.5 inches, 8.84 x 19.05 x 6.35 cm
Weight 13 oz, 362 g (meter only)
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STATISTICAL & TECHNICAL DEFINITIONS RELATED TO PRODUCT
SPECIFICATIONS
Method Detection Limit (MDL): “The method detection limit (MDL) is dened
as the minimum concentration of a substance that can be measured and
reported with 99% condence that the analyte concentration is greater than zero
and is determined from analysis of a sample in a given matrix containing the
analyte.”1 Note that, “As Dr. William Horwitz once stated, ‘In almost all cases when
dealing with a limit of detection or limit of determination, the primary purpose of
determining that limit is to stay away from it.’”
2
Accuracy: Accuracy is the nearness of a measurement to the accepted or true
value.3 The accuracy can be expressed as a range, about the true value, in
which a measurement occurs (i.e. ±0.5 ppm). It can also be expressed as the
% recovery of a known amount of analyte in a determination of the analyte (i.e.
103.5 %).
Resolution: Resolution is the smallest discernible difference between any
two measurements that can be made.4 For meters this is usually how many
decimal places are displayed. (i.e. 0.01). Note that the resolution many change
with concentration or range. In some cases the resolution may be less than the
smallest interval, if it is possible to make a reading that falls between calibration
marks. A word of caution, that resolution has very little relationship to accuracy or
precision. The resolution will always be less than the accuracy or precision but it
is not a statistical measure of how well a method of analysis works. The resolution
can be very, very good and the accuracy and precision can be very bad! This is
not a useful measure of the performance of a test method.
Repeatability: Repeatability is the within-run precision.5 A run is a single data
set, from set up to clean up. Generally, one run occurs on one day. However, for
meter calibrations, a single calibration is considered a single run or data set, even
though it may take 2 or 3 days.
Reproducibility: Reproducibility is the between-run precision.
6
Detection Limit (DL): The detection limit (DL) for the 2020we/wi
is dened as the minimum value or concentration that can be determined by the
meter, which is greater than zero, independent of matrix, glassware, and other
sample handling sources of error. It is the detection limit for the optical system of
the meter.
1
CFR 40, part 136, appendix B
2
Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta,
American Laboratory, Sept 2003, P. 31.
3
Skoog, D.A., West, D. M., Fundamental of Analytical Chemistry, 2nd ed., Holt
Rinehart and Winston, Inc, 1969, p. 26.
4
Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta,
American Laboratory, Sept 2003, P. 34.
5
Jeffery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of
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Quantitative Chemical Analysis, 5th ed., Longman Scientic & Technical, 1989, p.
130.
6
Jeffery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of
Quantitative Chemical Analysis, 5th ed., Longman Scientic & Technical, 1989, p.
130
CONTENTS AND ACCESSORIES
CONTENTS
COD3 Plus Colorimeter
Test Tubes, with Caps
COD/UDV Adapter
USB Wall Adapter
USB Cable
COD3 Plus Colorimeter Quick Start Guide
COD3 Plus Colorimeter Manual
ACCESSORIES
Test Tubes, with Caps Code 0290-6
Replacement Chamber Code 3-0038
USB Cable Code 1720
USB Wall Adapter Code 1721
COD/UDV Adapter Code 1724
Car Charger Code 5-0132
SMARTLink3 Program (CD) Code 1901-CD
Small Field Carrying Case
(37.5 27.5 x 13.75 cm)
Large Field Carrying Case
(45 x 32.5 x 20 cm)
Code 1910-GCS150
Code 1910-GCS440
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EPA COMPLIANCE
The COD3 Plus Colorimeter is an EPA-Accepted instrument. EPA-Accepted
means that the instrument meets the requirements for instrumentation as found
in test procedures that are approved for the National Primary Drinking Water
Regulations (NPDWR) or National Pollutant Discharge Elimination System
(NPDES) compliance monitoring programs. EPA-Accepted instruments may be
used with approved test procedures without additional approval.
CE COMPLIANCE
The COD3 Plus Colorimeter has earned the European CE Mark of Compliance for
electromagnetic compatibility and safety. The Declaration of Conformity for the
COD3 Plus Colorimeter is available at www.lamotte.com.
IP67 CERTIFICATION
The COD3 Plus meets IP67 standards for protection against dust and immersion
only when the USB port plug is in place. Documentation is available at www.
lamotte.com.
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CHEMICAL TESTING
WATER SAMPLING FOR CHEMICAL ANALYSIS
Taking Representative Samples
The underlying factor to be considered for any type of water sampling is whether
or not the sample is truly representative of the source. To properly collect a
representative sample:
• Sample as frequently as possible.
• Collect a large sample or at least enough to conduct whatever tests are
necessary.
• Make a composite sample for the same sampling area.
• Handle the sample in such a way as to prevent deterioration or
contamination before the analysis is performed.
• Perform analysis for dissolved gases such as dissolved oxygen, carbon
dioxide, and hydrogen sulde immediately at the site of sampling. Samples
for testing these factors, as well as samples for pH, cannot be stored for later
examination.
• Make a list of conditions or observations which may affect the sample. Other
considerations for taking representative samples are dependent upon the
source of the sample. Taking samples from surface waters involves different
considerations than taking samples from impounded and sub-surface
waters.
Sampling of Open Water Systems
Surface waters, such as those found in streams and rivers, are usually well mixed.
The sample should be taken downstream from any tributary, industrial or sewage
pollution source. For comparison purposes samples may be taken upstream and
at the source of the pollution before mixing.
In ponds, lakes, and reservoirs with restricted ow, it is necessary to collect a
number of samples in a cross section of the body of water, and where possible
composite samples should be made to ensure representative samples.
To collect samples from surface waters, select a suitable plastic container with
a tight tting screw cap. Rinse the container several times with the sample to be
tested, then immerse the container below the surface until it is lled to overowing
and replace the cap. If the sample is not to be tested immediately, pour a small
part of the sample out and reseal. This will allow for any expansion. Any condition
which might affect the sample should be listed.
Sub-surface sampling is required to obtain a vertical prole of streams, lakes,
ponds, and reservoirs at specic depths. This type of sampling requires more
sophisticated sampling equipment.
For dissolved oxygen studies, or for tests requiring small sample sizes, a Water
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Sampler (LaMotte Code 1060) will serve as a subsurface or in-depth sampler.
This weighted device is lowered to the sampling depth and allowed to rest at
this depth for a few minutes. The water percolates into the sample chamber
displacing the air which bubbles to the surface. When the bubbles cease to rise,
the device has ushed itself approximately ve times and it may be raised to
the surface for examination. The inner chamber of the sampling device is lifted
out and portions of the water sample are carefully dispensed for subsequent
chemical analysis.
A Snap-Plunger Water Sampler (LaMotte Code 1077) is another “in-depth”
sampling device which is designed to collect large samples which can be used
for a multitude of tests. Basically, this collection apparatus is a hollow cylinder
with a spring loaded plunger attached to each end. The device is cocked
above the surface of the water and lowered to the desired depth. A weighted
messenger is sent down the calibrated line to trip the closing mechanism and the
plungers seal the sample from mixing with intermediate layers as it is brought to
the surface. A special drain outlet is provided to draw off samples for chemical
analysis.
Sampling of Closed System
To obtain representative samples from conned water systems, such as
pipe lines, tanks, vats, lters, water softeners, evaporators and condensers,
different considerations are required because of chemical changes which occur
between the inlet and outlet water. One must have a basic understanding of the
type of chemical changes which occur for the type of equipment used. Also,
consideration should be given to the rate of passage and retaining time for the
process water.
Temperature changes play an important part in deciding exactly what test should
be performed. Process water should be allowed to come to room temperature,
20–25°C, before conducting any tests.
When drawing off samples from an outlet pipe such as a tap, allow sample to
run for several minutes, rinsing the container several times before taking the nal
sample. Avoid splashing and introduction of any contaminating material.
FILTRATION
When testing natural waters that contain signicant turbidity due to suspended
solids and algae, ltration is an option. Reagent systems, whether EPA, Standard
Methods, LaMotte or any others, will generally only determine dissolved
constituents. Both EPA and Standard Methods suggest ltration through a 0.45
micron lter membrane, to remove turbidity, for the determination of dissolved
constituents.** To test for total constituents, organically bound and suspended or
colloidal materials, a rigorous high temperature acid digestion is necessary.
**LaMotte offers a ltering apparatus: syringe assembly (Code 1050) and
membrane lters, 0.45 micron, (Code 1103).
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AN INTRODUCTION TO COLORIMETRIC ANALYSIS
Most test substances in water are colorless and undetectable to the human eye.
To test for their presence we must nd a way to “see” them. The COD3 Plus
Colorimeter can be used to measure any test substance that is itself colored or
can be reacted to produce a color. In fact a simple denition of colorimetry is
“the measurement of color” and a colorimetric method is “any technique used
to evaluate an unknown color in reference to known colors”. In a colorimetric
chemical test the intensity of the color from the reaction must be proportional to
the concentration of the substance being tested. Some reactions have limitations
or variances inherent to them that may give misleading results. Many such
interferences are discussed with each particular test instruction. In the most basic
colorimetric method the reacted test sample is visually compared to a known
color standard. However, accurate and reproducible results are limited by the
eyesight of the analyst, inconsistencies in the light sources, and the fading of
color standards.
To avoid these sources of error, a colorimeter can be used to photoelectrically
measure the amount of colored light absorbed by a colored sample in reference
to a colorless sample (blank).
White light is made up of many different colors or wavelengths of light. A colored
sample typically absorbs only one color or one band of wavelengths from the
white light. Only a small difference would be measured between white light before
it passes through a colored sample versus after it passes through a colored
sample. The reason for this is that the one color absorbed by the sample is only
a small portion of the total amount of light passing through the sample. However,
if we could select only that one color or band of wavelengths of light to which the
test sample is most sensitive, we would see a large difference between the light
before it passes through the sample and after it passes through the sample.
The COD3 Plus Colorimeter passes one of four colored light beams through
one of four optical lters which transmits only one particular color or band of
wavelengths of light to the photodectector where it is measured. The difference
in the amount of colored light transmitted by a colored sample is a measurement
of the amount of colored light absorbed by the sample. In most colorimetric tests
the amount of colored light absorbed is directly proportional to the concentration
of the test factor producing the color and the path length through the sample.
However, for some tests the amount of colored light absorbed is inversely
proportional to the concentration.
The choice of the correct wavelength for testing is important. It is interesting to
note that the wavelength that gives the most sensitivity (lower detection limit)
for a test factor is the complementary color of the test sample. For example the
Nitrate-Nitrogen test produces a pink color proportional to the nitrate-nitrogen
concentration in the sample (the greater the nitrate-nitrogen concentration, the
darker the pink color). A wavelength in the green region should be selected to
analyze this sample since a pinkish-red solution absorbs mostly green light.
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REAGENT BLANK
Some tests will provide greater accuracy if a reagent blank is determined to
compensate for any color or turbidity resulting from the reagents themselves. A
reagent blank is performed by running the test procedure on demineralized or
deionized water. Use sample water to SCAN BLANK. Insert the reacted reagent
blank in the colorimeter chamber and select SCAN SAMPLE. Note result of
reagent blank. Perform the tests on the sample water as described. Subtract
results of reagent blank from all subsequent test results. NOTE: Some tests
require a reagent blank to be used to SCAN BLANK.
COLORIMETER TUBES AND CHAMBER
Colorimeter tubes and colorimeter chambers which have been scratched through
excessive use should be discarded and replaced with new ones. Dirty tubes
should be cleaned on both the inside and outside. Fingerprints on the exterior
of the tubes can cause excessive light scattering and result in errors. Handle the
tubes carefully, making sure the bottom half of the tube is not handled.
LaMotte Company makes every effort to provide high quality colorimeter tubes.
However, wall thicknesses and diameter of tubes may still vary slightly. This may
lead to slight variations in results (e.g. if a tube is turned while in the sample
chamber, the reading will likely change slightly). To eliminate this error put the
tubes into the sample chamber with the same orientation every time.
The tubes that are included with the colorimeter have an index mark to facilitate
this. If possible, use the same tube to SCAN BLANK and SCAN SAMPLE.
METER CARE
The optical system of the COD3 Plus must be kept clean and dry for optimal
performance. Dry the colorimeter tubes before placing them in the chamber to
avoid introducing moisture. For best results store the instrument in a area that is
dry and free from aggressive chemical vapors.
SELECTING AN APPROPRIATE WAVELENGTH
The most appropriate wavelength to use when creating a calibration curve
is usually the one which gives the greatest change from the lowest reacted
standard concentration to the highest reacted standard concentration. However,
the absorbance of the highest reacted standard concentration should never
be greater than 2.0 absorbance units. Scan the lowest and highest reacted
standards at different wavelengths using the absorbance mode to nd the
wavelength which gives the greatest change in absorbance without exceeding 2.0
absorbance units. Use this wavelength to create a calibration curve.
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Below is a list of suggested wavelengths for the color of the reacted samples. Use
these as a starting point.
Sample Color Wavelength Range
Yellow 428
Pink 525
Red 568
Green and Blue 635
NOTE: Available wavelengths in the COD3 Plus are 428 nm and 635 nm.
CALIBRATION
As with all pre-calibrated meters, it is highly recommended, even if not required
by regulations, that the user periodically verify the performance of the meter
by running standards with a predetermined concentration. Results outside of
specication are an indication that the meter needs to be adjusted. This can be
done following the user calibration described on page 32. If the user calibration
fails to properly adjust the meter then the meter should be returned to LaMotte
Company for recalibration. (See page 68).
CALIBRATION CURVES
The COD3 Plus Colorimeter contains tests for the LaMotte reagent systems. The
rst step in using a non-LaMotte reagent system with your COD3 Plus Colorimeter
is to create a calibration curve for the reagent system. To create a calibration
curve, prepare standard solutions of the test factor and use the reagent system to
test the standard solutions with the COD3 Plus Colorimeter. Select a wavelength
for the test as described above.
Plot the results (in ABS or %Transmittance) versus concentration to create
a calibration curve. The calibration curve may then be used to identify the
concentration of an unknown sample by testing the unknown, reading
Absorbance or %T, and nding the corresponding concentration from the curve.
The linear range of the reagent system can be determined and this information
can be used to input a User Test into the COD3 Plus Colorimeter (see Edit User
Tests, page 39).
PROCEDURE
Prepare 5 or 6 standard solutions of the factor being tested. The concentration of
these standards should be evenly distributed throughout the range of the reagent
system, and should include a 0 ppm standard (distilled water). For instance,
the solutions could measure 0, 10%, 30%, 50%, 70%, and 90% of the system’s
maximum range.
1. Turn on the COD3 Plus Colorimeter. Select the appropriate wavelength from
the absorbance mode. Be sure to select the appropriate wavelength for the
color produced by the reagent system.
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2. Use the unreacted 0 ppm standard to standardize the colorimeter by using it
to scan blank.
3. Following the individual reagent system instructions, react each standard
solution beginning with 0 ppm. Continue with standards in increasing
concentration. Record the reading and the standard solution concentration
on a chart. Readings can be recorded as percent transmittance (%T) or
absorbance (A).
4. Plot results on graph paper or computer using any available plotting program.
If results are as %T versus concentration, semilog graph paper must be
used. Plot the standard solution concentrations on the horizontal, linear axis,
and the %T on the vertical, logarithmic axis. If results are as absorbance
versus standard solution concentration, simple linear graph paper can be
used. Plot the standard solution concentration on the horizontal axis, and the
absorbance on the vertical axis.
5. After plotting the results, draw a line, or curve, of best t through the
plotted points. The best t may not connect the points. There should be
approximately an equal number of points above the curve as below the curve.
Some reagent systems will produce a straight line, while others produce a
curve. Many computer spreadsheet programs can produce the curve of best
t by regression analysis of the standard solution data.
NOTE: Only reagent systems which produce a straight line can be used for a
User Test.
A sample of each type of graph appears below:
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PREPARING DILUTE STANDARD SOLUTIONS
Standard solutions should be prepared to create a calibration curve. Standard
solutions can be prepared by diluting a known concentrated standard by
specied amounts. A chart or computer spreadsheet can be created to determine
the proper dilutions. Use volumetric asks and volumetric pipets for all dilutions.
1. In Column A – Record the maximum concentration of test as determined by
the range and path length.
2. In Column B – Record the percent of the maximum concentration the
standard solution will be.
3. In Column C – Calculate the nal concentration of the diluted standard
solutions by multiplying the maximum concentration (In Column A) by the %
of maximum concentration divided by 100. (C = A x
4. In Column D – Record the nal volume of the diluted sample (i.e. volume of
volumetric ask).
5. In Column E – Record the concentration of the original standard.
6. In Column F – Calculate the milliliters of original standard required (F = (C x
D
/E)).
A sample chart appears below:
B
100
/
).
A B C =
Maximum
concentration
of test
% of Maximum
concentration
100
A x B/
concentration
of Diluted
Standard
Final
D E F =
Volume of
Standard
Concentration
of Original
Standard
C x D/E
mL of
Original
Standard
Required
10.0 ppm 90 9.0 ppm 100 mL 1000 ppm 0.90 mL
10.0 ppm 70 7.0 ppm 100 mL 1000 ppm 0.70 mL
10.0 ppm 50 5.0 ppm 100 mL 1000 ppm 0.50 mL
10.0 ppm 30 3.0 ppm 100 mL 1000 ppm 0.30 mL
10.0 ppm 10 1.0 ppm 100 mL 1000 ppm 0.10 mL
10.0 ppm 0 0 ppm 100 mL 1000 ppm 0 mL
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STANDARD ADDITIONS
A common method to check the accuracy and precision of a test is by standard
additions. In this method a sample is tested to determine the concentration of
the test substance. A second sample is then “spiked” by the addition of a known
quantity of the test substance. The second sample is then tested. The determined
concentration of the spiked sample should equal the concentration of the first plus
the amount added with the spike. The procedure can be repeated with larger and
larger “spikes.” If the determined concentrations do not equal the concentration of
the sample plus that added with the “spike”, then an interference may exist.
For example, a 10.0 mL water sample was determined to contain 0.3 ppm iron.
To a second 10.0 mL sample, 0.1 mL of 50 ppm iron standard was added. The
concentration of iron due to the “spike” was (0.10 mL x 50 ppm)/10.0 mL = 0.50
ppm. The concentration of iron determined in the spiked sample should be 0.3 +
0.5 = 0.8 ppm iron. (Note: any error due to the increased volume from the “spike” is
negligible).
LaMotte offers a line of calibration standards which can be used to generate
calibration curves and perform standard additions.
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SAMPLE DILUTION TECHNIQUES & VOLUMETRIC
MEASUREMENTS
If a test result using the COD3 Plus Colorimeter gives an over range message
then the the sample must be diluted. The test should be repeated on the diluted
sample to obtain a reading which is in the concentration range for the test. (Note:
This is not true for colorimetric determination of pH.)
Example:
Measure 5 mL of the water sample into a graduated cylinder. Add
demineralized water until the cylinder is lled to the 10 mL line. The
sample has been diluted by one-half, and the dilution factor is therefore
2. Perform the test procedure, then multiply the resulting concentration
by 2 to obtain the test result.
The following table gives quick reference guidelines on dilutions of various
proportions. All dilutions are based on a 10 mL volume, so several dilutions will
require small volumes of the water sample. Graduated pipets should be used for
all dilutions.
Deionized Water to
Size of Sample
10 mL 0 mL 1
5 mL 5 mL 2
2.5 mL 7.5 mL 4
1 mL 9 mL 10
0.5 mL 9.5 mL 20
Bring Volume to 10 mL Multiplication Factor
If the above glassware is not available, dilutions can be made with the colorimeter
tube. Fill the tube to the 10 mL line with the sample then transfer it to another
container. Add 10 mL volumes of demineralized water to the container and
mix. Transfer back 10 mL of the diluted sample to the tube and follow the
test procedure. Continue diluting and testing until a reading, which is in the
concentration range for the test, is obtained. Be sure to multiply the concentration
found by the dilution factor (the number of total 10 mL volumes used).
Example:
10 mL of sample is diluted with three 10 mL volumes of demineralized
water; the dilution factor is four.
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INTERFERENCES
LaMotte reagent systems are designed to minimize most common interferences.
Each individual test instruction discusses interferences unique to that test. Be
aware of possible interferences in the water being tested.
The reagent systems also contain buffers to adjust the water sample to the ideal
pH for the reaction. It is possible that the buffer capacity of the water sample may
exceed the buffer capacity of the reagent system and the ideal pH will not be
obtained. If this is suspected, measure the pH of a reacted distilled water reagent
blank using a pH meter. This is the ideal pH for the test. Measure the pH of a
reacted water sample using the pH meter. If the pH is signicantly different from
the ideal value, the pH of the sample should be adjusted before testing.
Interferences due to high concentration of the substance being tested, can be
overcome by sample dilution (see page 19)
STRAY LIGHT INTERFERENCE
When scanning samples in 16 mm tubes, such as COD, the sample chamber lid
can not be closed. The COD adapter minimizes stray light. To further reduce stray
light interference, do not scan sample in direct sunlight.
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OPERATION OF THE
COD3 PLUS COLORIMETER
OVERVIEW
The COD3 Plus is a portable, microprocessor controlled, direct reading
colorimeter. It has a graphical liquid crystal display and 6 button keypad. These
allow the user to select options from the menu driven software, to directly read
test results or to review stored results of previous tests in the data logger. The
menus can be displayed in seven different languages.
The test library consists of 29 LaMotte tests and 25 “User Tests”. The LaMotte
tests are precalibrated for LaMotte reagent systems.The colorimeter displays the
result of these tests directly in units of concentration. The 25 “User Tests” may be
used to enter additional calibrations. All of these tests may be arranged in any of
3 sequences. These sequences can be modied a limitless number of times to
meet changing testing needs.
The optics feature 2 different colored LEDs. Each LED has a corresponding
silicon photoiode with an integrated interference lter. The interference lters
select a narrow band of light from the corresponding LED for the colorimetric
measurements. The microporcessor automatically selects the correct LED/
photodiode combination for the test.
A USB wall adapter, USB computer connection or lithium battery powers the
COD3 Plus.
A USB port on the back of the meter allows an interface of the meter with a
Windows-based computer for real-time data acquisition and data storage using
a PC. The COD3 Plus may be interfaced with any Windows-based computer by
using the LaMotte SMARTLink3 Program.
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COMPONENTS
Figure 1 shows a diagram of the COD3 Plus Colorimeter and its components.
COD3 Plus
COLORIMETER
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GENERAL OPERATING PROCEDURES
The operation of the COD3 Plus Colorimeter is controlled by a microprocessor.
The microprocessor is programmed with menu driven software. A menu is a list
of choices. This allows a selection of various tasks for the colorimeter to perform,
such as, scan blank, scan sample, and edit test sequences. The keypad is
used to make menu selections which are viewed in the display. There are three
selections accessible from the Main Menu: Testing Menu, Editing Menu and Run
PC Link.
THE KEYPAD
The keypad has 6 buttons which are used to perform specic tasks.
This button will scroll up through a list of menu selections.
ENTER
The button is used to select choices in a menu viewed in
the display.
This button controls the backlight on the display.
This button will scroll down through a list of menu
selections.
EXIT
This button exits to the previous menu.
This button turns the meter on or off.
ENTER
EX IT
SAMPLE HOLDERS
The sample chamber is designed for 25 mm round
tubes. An adapter to hold 16 mm COD tubes and 1
cm square UDV cuvettes is included.
Position the COD/UDV Adapter (Code 1724) so
that the notches in the adapter t around the posts
on the chamber. Turn the adapter counterclockwise
until the arrows are at the top and bottom of the
chamber and the adapter is locked into place. Turn
the adapter clockwise to unlock the adapter and
remove it from the chamber.
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THE DISPLAY & THE MENUS
The display allows menu selections to be viewed and selected. These selections
instruct the COD3 Plus to perform specic tasks. The menus are viewed in the
display using two general formats that are followed from one menu to the next.
Each menu is a list of choices or selections.
The display has a header line at the top and a footer line at the bottom. The
header displays the title of the current menu. The footer line displays the time
and the date, the data logger status and the battery status. The menu selection
window is in the middle of the display between the header and the footer.
The menu selection window displays information in two general formats. In the
rst format only menu selections are displayed. Up to 4 lines of menu selections
may be displayed. If more selections are available they can be viewed by
pressing the arrow buttons to scroll the other menu selections into
the menu selection window. Think of the menu selections as a vertical list in the
display that moves up or down each time an arrow button is pressed.
Some menus in the COD3 Plus are looping menus. The top and bottom menu
choices are connected in a loop. Scrolling down past the bottom of the menu will
lead to the top of the menu. Scrolling up past the top of the menu will lead to the
bottom of the menu.
Header Menu Title
Main Window Selection First Choice
Second Choice
Third Choice
Another
Footer
12:00:00 001/500
And Another
And So On
A light bar will indicate the menu choice. As the menu is scrolled through, the
ENTER
light bar will highlight different menu choices. Pressing the
button will
select the menu choice that is indicated by the light bar.
In the second format the menu choice window takes advantage of the graphical
capabilities of the display. Large format graphic information, such as test results
or error messages or the LaMotte logo is displayed. The top two lines of the
display are used to display information in a large, easy to read format. The menus
work in the same way as previously described but two lines of the menu are
visible at the bottom of the display.
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Header Menu Title
Message or Result Window
Result or
Message
Another
Main Window Selection And Another
Footer
As described previously, the
current menu and a return to the previous menu. This allows a rapid exit from an
inner menu to the main menu by repeatedly pushing the
at any time will turn the COD3 Plus off.
The display may show the following messages:
Battery Status
More choices are available and can be viewed by
scrolling up and/or down through the display.
EXIT
12:00:00 001/500
And So On
Last Choice
button allows an exit or escape from the
EXIT
button. Pushing
Header Identies the current menu and information on units
and reagent systems if applicable.
Footer In the data logging mode the number of the data point
is displayed and the total number of data points in the
memory will be shown. The footer also shows current
time and battery status
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LOOPING MENUS
Long menus, such as All Tests, incorporate a looping feature which allows the
user to quickly reach the last choice in the menu from the rst choice. In a looping
menu the last choices in the menu are above the rst choice and scrolling upward
moves through the menu in reverse order. Scrolling downward moves through the
menu from rst choice to last but the menu starts over following the last choice.
So all menu choices can be reached by scrolling in either direction. The diagrams
below demonstrate a looping menu.
AND SO ON AND SO ON AND SO ON
: : : : : : : : :
: : : : : : : : :
THIRD TO LAST LAST CHOICE LAST CHOICE
SECOND TO LAST AND SO ON AND SO ON
LAST CHOICE : : : : : :
TESTING MENU TESTING MENU TESTING MENU
FIRST CHOICE : : : : : :
SECOND CHOICE THIRD TO LAST THIRD TO LAST
THIRD CHOICE SECOND TO LAST SECOND TO LAST
ANOTHER LAST CHOICE LAST CHOICE
12:00:00 001/500 12:00:00 001/500 12:00:00 001/500
AND ANOTHER FIRST CHOICE FIRST CHOICE
AND SO ON SECOND CHOICE SECOND CHOICE
: : : THIRD CHOICE THIRD CHOICE
: : : ANOTHER ANOTHER
LAST CHOICE AND ANOTHER AND ANOTHER
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TESTING
TESTING MENU
The Testing Menu is used to run all LaMotte pre-programmed tests, User Tests
and Absorbance tests at one of two wavelengths. Testing from any of three
sequences can also be done.
1. Press and briey hold
to turn the meter on. The
LaMotte logo screen will
appear for about 3 seconds
and the Main Menu will
appear.
2. Press
ENTER
to select Testing
Menu.
3. Press or to scroll
to desired option. All Tests
contains all of the available
pre-programmed tests. The
three sequences have user
selected tests. Absorbance
has %T/ABS tests.
Main Menu
Testing Menu
Editing Menu
Run PC Link
12:00:00 001/500
Testing Menu
All Tests Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
Testing Menu
All Tests Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
4. Press
option.
ENTER
to select the
001 Alkalinity UDV
All Tests
002 Aluminum
003 Ammonia-N LRF
004 Ammonia-N LRS
12:00:00 001/500
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TEST SEQUENCES
Sequence 1, Sequence 2, And Sequence 3 are alterable sequences. They may
be edited using the Editing Menu. Any of the LaMotte pre-programmed tests or
User Tests may be placed in these sequences in whatever testing order that is
preferred. Some examples of typical sequences are given below.
NOTE: Test in the examples may not be included in the COD3 Plus.
Sequence 1 Sequence 2 Sequence 3
015 Chlorine F UDV 002 Aluminum 003 Ammonia-N LRF
079 Phosphate HR 035 Cyanuric Acid 032 Cu UDV
009 Benzotriazole 053 Iron Phenanthro 064 Nitrate-N LR
076 pH UDV 055 Manganese LR 067 Nitrite-N LR
12:00:00 001/500
061 Molybdenum HR 064 Nitrate-N LR 074 pH PR
086 Silica HR 067 Nitrite-N LR 078 Phosphate LR
045 Hydrazine 077 Phenol 085 Silica LR
032 Cu UDV 078 Phosphate LR
051 Iron Bipyridyl 090 Sulde LR
These alterable sequences allow a series of tests to be setup that are run
frequently. The order of the individual tests in the sequence is determined by the
user. After running a test, press
Continue this pattern until the entire sequence has been completed.
12:00:00 001/500 12:00:00 001/500
ENTER
to select the next test in the sequence.
All Tests is a xed sequence containing the LaMotte pre-programmed tests, User
Tests, and Absorbance tests.
Modication of the alterable sequences is accomplished through the Editing
Menu. This menu is explained in greater detail in Editing Menu (p. 39).
Pressing
EXIT
while in a sequence menu will escape back to the Testing Menu.
Pressing the at any time will turn the colorimeter off.
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GENERAL TESTING PROCEDURES
The following are some step by step examples of how to run tests from the
Testing Menu. These test procedures are designed to be used with LaMotte
SMART Reagent Systems.
LaMotte Company continuously updates the list of pre-programmed tests as the
calibrations become available. Pre-programmed calibrations can be added to
the COD3 Plus Colorimeter in the eld. A Windows-based computer running a
Windows Operating System is required.
Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the
USA) or email at tech@lamotte.com for a current list of available calibrations and
downloading instructions.
TESTING WITH LaMOTTE PRE-PROGRAMMED TESTS
NOTE: Test in the examples may not be included in the COD3 Plus.
1. Press and briey hold
to turn the meter on. The
LaMotte logo screen will
appear for about 3 seconds
and the Main Menu will
appear.
2. Press
ENTER
to select Testing
Menu.
3. Press
ENTER
to select All Tests
Menu.
Main Menu
Testing Menu
Editing Menu
Run PC Link
12:00:00 001/500
Testing Menu
All Test Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
All Tests
001 Alkalinity UDV
002 Aluminum
003 Ammonia-N LRF
004 Ammonia-N LRS
12:00:00 001/500
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4. Press or to scroll
to the desired test.
All Tests
001 Alkalinity UDV
002 Aluminum
003 Ammonia-N LRF
004 Ammonia-N LRS
12:00:00 001/500
5. Press
ENTER
to select the test.
6. Insert the blank into the
chamber. Close the lid.Press
ENTER
to scan the blank. The
screen wil display Blank
Done for about 1 second and
then return to the Test Menu.
7. Insert the reacted sample
into the chamber. Close
the lid. Press
ENTER
to scan
the sample. The screen will
display READING for about 1
second. The result will appear
on the screen.
002 Aluminum
Scan Bank
Scan Sample
12:00:00 001/500
002 Aluminum
Scan Blank
Scan Sample
12:00:00 001/500
002 Aluminum
1.00 ppm
Scan Blank
Scan Sample
12:00:00 001/500
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8. To repeat the test, press
to scan the sample again. The
last blank scaned is used by
the colorimeter for repeated
scans. A different blank can
be used by pressing or
to scroll to Scan Blank
and then scanning another
blank. Scroll with or
and make another
selection with
or Absorbance of the last test
can be viewed by choosing
%T/Abs. Press
escape to previous menus.
NOTE: The menus loop in
this screen so either or
will lead to the menu
selection needed.
ENTER
EXIT
. The %T
to
ENTER
002 Aluminum
1.00 ppm
Scan Bank
Scan Sample
12:00:00 001/500
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CALIBRATING LaMOTTE PRE-PROGRAMMED TESTS
The LaMotte Pre-Programmed Tests have been pre-calibrated. Recalibration of
the pre-programmed tests by the user is not possible. However, a procedure
to standardize the calibration can be performed to obtain the most accurate
readings or to meet regulatory requirements.
The LaMotte Pre-Programmed tests are standardized with one standard solution.
To standardize over the full range of the test, the concentration of the standard
should be chosen from the high end of the range. Alternatively, if samples do not
cover the full range of the test, a standard should be chosen that is close to the
concentration of the samples.
The standardization procedure should be followed as often as required by
regulations and laws for compliance monitoring.
In the example below, the Aluminum calibration will be standardized.
Prepare a standard solution to be tested. In this example, 0.30 ppm aluminum.
NOTE: Aluminum testis used as an example but is not available in the
COD3 Plus.
1. Press and briey hold
to turn the meter on. The
LaMotte logo screen will
appear for about 3 seconds
and the Main Menu will
appear.
2. Press
ENTER
to select Testing
Menu.
Main Menu
Testing Menu
Editing Menu
Run PC LINK
12:00:00 001/500
Testing Menu
All Test Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
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3. Press
Menu.
ENTER
to select All Tests
All Tests
001 Alkalinity UDV
002 Aluminum
003 Ammonia-N LRF
004 Ammonia-N LRS
12:00:00 001/500
4. Press or to scroll
to the desired test factor.
5. Press
ENTER
to select the test.
6. Follow the test procedure
in the manual to test the
prepared standard. Insert the
blank into the chamber. Close
the lid. Press
ENTER
to scan
the blank. The screen will
display Blank Done for about
1 second and then return to
the Test Menu.
All Tests
001 Alkalinity UDV
002 Aluminum
003 Ammonia-N LRF
004 Ammonia-N LRS
12:00:00 001/500
002 Aluminum
Scan Blank
Scan Sample
12:00:00 001/500
002 Aluminum
Scan Blank
Scan Sample
12:00:00 001/500
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7. Insert the reacted standard
solution into the chamber.
Close the lid. Press
scan the sample. The screen
will display Reading for about
1 second. The result will
appear on the screen.
ENTER
to
002 Aluminum
0.28 ppm
Scan Blank
Scan Sample
12:00:00 001/500
8. The displayed result can now
be standardized. Press
or to scroll to calibrate.
9. Press
Calibrate. A reverse font
(light background with dark
characters) will appear to
indicate that the reading can
be adjusted.
10. Press or to scroll
to the concentration of the
prepared standard, 0.30 in
this example.
NOTE: A maximum
adjustment of 10% is possible.
If an adjustment of over 10%
is attempted, Overrange will
be displayed.
ENTER
to select
002 Aluminum
0.28 ppm
%T/Abs
Calibrate
12:00:00 001/500
002 Aluminum
0.28 ppm
%T/Abs
Calibrate
12:00:00 001/500
002 Aluminum
0.30 ppm
%T/Abs
Calibrate
12:00:00 001/500
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11. Press
Calibrate. Two menu choices
will be offered, set calibration
and factory setting.
ENTER
to select
002 Aluminum
0.30 ppm
Set Calibration
Factory Setting
12:00:00 001/500
12. Press
Calibration and save the
calibration. Or press
to scroll to Factory Setting.
Press
Setting to revert to the factory
calibration. The screen will
display Storing... for about
1 second and the test menu
will appear. The calibration
has now been standardized
and the meter can be used for
testing. The standardization
can be removed by repeating
the calibration and selecting
Factory Setting.
ENTER
to select Set
ENTER
to select Factory
002 Aluminum
Scan Blank
Scan Sample
12:00:00 001/500
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MEASURING IN THE ABSORBANCE MODE
1. Press and briey hold
to turn the meter on. The
LaMotte logo screen will
appear for about 3 seconds
and the Main Menu will
appear.
2. Press
ENTER
to select Testing
Menu.
3. Press or to scroll
to Absorbance.
Main Menu
Testing Menu
Editing Menu
Run PC Link
12:00:00 001/500
Testing Menu
All Test Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
Testing Menu
Sequence 1
Sequence 2
Sequence 3
Absorbance
12:00:00 001/500
4. Press
Absorbance.
ENTER
to select
101 Absorbance 428
Absorbance
102 Absorbance 525
103 Absorbance 568
104 Absorbance 635
12:00:00 001/500
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5. Press or to scroll
to desired wavelength.
Absorbance
101 Absorbance 428
102 Absorbance 525
103 Absorbance 568
104 Absorbance 635
12:00:00 001/500
6. Press
ENTER
to select the
wavelength.
7. Insert the blank. Close the lid.
Press
ENTER
to scan the blank.
The screen wil display Blank
Done for about 1 second
and return to the Absorbance
menu.
8. Insert the reacted sample.
Press
ENTER
to scan the
sample. The screen will
display Reading for about 1
second. The result will appear
on the screen.
102 Absorbance 525
Scan Blank
Scan Sample
12:00:00 001/500
102 Absorbance 525
Scan Blank
Scan Sample
12:00:00 001/500
102 Absorbance 525
0.425
Scan Blank
Scan Sample
12:00:00 001/500
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9. To repeat the test, press
to scan the sample again. The
last blank scanned is used by
the colorimeter for repeated
scans. A different blank can
be used by pressing or
to scroll to Scan Blank
and then scanning another
blank. Scroll with or
and make another
selection with
Absorbance of the last test can
be viewed by choosing
%T/Abs. Press
escape to previous menus.
NOTE: The menus loop in
this screen so either or
will lead to the menu
selection needed.
NOTE: The calibrate function
does not work in the
Absorbance mode.
ENTER
EXIT
.The %T or
to
ENTER
102 Absorbance 525
0.425
Scan Blank
Scan Sample
12:00:00 001/500
Next Test
Previous Test
%T/Abs
Calibrate
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EDITING MENU
The Editing Menu allows the user to edit sequences, edit user tests, set the clock,
edit the logging function, access factory setting, set the power saving function,
set the backlight time, and select a language.
The default factory settings are:
Date Format MM-DD-YYYY
Logging Enabled
Power Save 5 minutes
Backlight 10 seconds
Language English
EDITING A SEQUENCE
The Edit Sequence menu allows three alterable test sequences (Sequence 1,
Sequence 2, Sequence 3) to be edited.
1. Press and briey hold
to turn the meter on. The
LaMotte logo screen will
appear for about 3 seconds
and the Main Menu will
appear.
2. Press or to scroll
to the Editing Menu.
3. Press
ENTER
to select Editing
Menu.
Main Menu
Testing Menu
Editing Menu
Run PC Link
12:00:00 001/500
Main Menu
Testing Menu
Editing Menu
Run PC Link
12:00:00 001/500
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
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4. Press
Sequences.
ENTER
to select Edit
Edit Sequences
Edit Sequence 1
Edit Sequence 2
Edit Sequence 3
12:00:00 001/500
5. Press or to scroll
to the desired sequence.
6. Press
ENTER
to select the
sequence to be edited.
Edit Sequences
Edit Sequence 1
Edit Sequence 2
Edit Sequence 3
12:00:00 001/500
EDIT SEQUENCE 2
015 Chlorine F UDV
079 Phosphate HR
009 Benzotriazole
076 pH UDV
12:00:00 001/500
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ADDING OR DELETING A TEST
There are three ways to alter a sequence: Insert Before, Insert After, and Delete.
Insert Before adds a new test to the sequence before the selected test. Insert
After adds a new test to the sequence after the selected test. Delete is used to
remove an existing test from a sequence.
NOTE: Test in the examples may not be included in the COD3 Plus.
ADDING A TEST
Below is a step-by-step example of how to add a test to SEQUENCE 2 starting
from the EDIT SEQUENCE 2 menu.
1. To add a test before or after
an existing test, press
or to scroll to the
existing test.
2. Press
ENTER
to select the
existing test.
3. Press or to scroll
to Insert Before or Insert
After.
EDIT SEQUENCE 2
015 Chlorine F UDV
079 Phosphate HR
009 Benzotriazole
076 pH UDV
12:00:00 001/500
Add or Delete
Insert Before
Insert After
Delete
12:00:00 001/500
Add or Delete
Insert Before
Insert After
Delete
12:00:00 001/500
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4. Press
ENTER
to select the
option, Insert Before, in this
example. The All Test Menu
will appear.
All Tests
001 Alkalinity
002 Aluminum
003 Ammonia-N LRF
004 Ammonia-N LRS
12:00:00 001/500
5. Press or to scroll
to the test that will be added
to the sequence. In this
example, Aluminum.
6. Press
ENTER
to select the test.
The sequence will appear in
the Edit Sequence menu and
the new test will be added to
the sequence. All changes
in the sequence will be
automatically saved.
7. Press
EXIT
to exit the Edit
Sequence menu and return
to the Editing Menu.
All Tests
001 Alkalinity UDV
002 Aluminum
003 Ammonia-N LRF
004 Ammonia-N LRS
12:00:00 001/500
EDIT SEQUENCE 2
015 Chlorine F UDV
079 Phosphate HR
002 Aluminum
009 Benzotriazole
12:00:00 001/500
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
8. Press
Sequences to continue
editing the sequences or
press
Main Menu.
42 COD3 Plus Colorimeter 11.07
ENTER
to select Edit
EXIT
to return to the
Testing Menu
Editing Menu
Run PC Link
12:00:00 001/500
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Page 43
DELETING A TEST
Below is a step-by-step example of how to delete a test in SEQUENCE 2 starting
from the EDIT SEQUENCE 2 menu.
1. To delete a test, press
or to scroll to the test in
the sequence.
2. Press
ENTER
to select the test.
3. Press or to scroll
to Delete.
EDIT SEQUENCE 2
015 Chlorine F UDV
079 Phosphate HR
002 Aluminum
009 Benzotriazole
12:00:00 001/500
Add or Delete
Insert Before
Insert After
Delete
12:00:00 001/500
Add or Delete
Insert Before
Insert After
Delete
12:00:00 001/500
4. Press
ENTER
to select Delete.
The sequence will appear
in the EDIT SEQUENCE
menu and the selected test
will have been deleted. All
015 Chlorine F UDV
079 Phosphate HR
002 Aluminum
EDIT SEQUENCE 2
changes to the sequence
will automatically have been
saved.
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Page 44
5. Press
EXIT
to exit the Edit
Sequence menu and return
to the Editing Menu.
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
6. Press
ENTER
to select Edit
Sequences to continue
editing the sequences or
press
EXIT
to return to the
Main Menu.
Main Menu
Testing Menu
Editing Menu
Run PC Link
12:00:00 001/500
44 COD3 Plus Colorimeter 11.07
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EDIT USER TESTS
If a test other than the LaMotte programmed tests is performed regularly, a
calibration for it may be entered in one of the 25 User Tests. These tests are
originally named “User Test 1 - 25”. It will be possible to rename the test, select a
wavelength, enter a new calibration, select the number of decimal places used to
display the results, and select the units. A User Test may be added for a reagent
system for which no precalibrated test exists. A calibration of a LaMotte reagent
system may also be entered. The calibration of a User Test can be changed at
any time.
The User Tests have the ability to handle 2 data points. The colorimeter will
determine the absorbance of the standards and calculate a response that
will be stored to determine the concentration of future samples of unknown
concentration. These standards should cover all the concentrations for the range
of the test being performed and be scanned beginning with the low concentration
and nishing with the high concentration (for more information about this, see
CALIBRATION CURVES, page 12). Prepare these standards prior to entering a
new calibration.
NOTE: A calibration procedure must be performed before using any of the User
Tests.
The User Tests can be placed in any of the alterable sequences using Edit
Sequences.
1. Press and briey hold
to turn the meter on. The
LaMotte logo screen will
appear for about 3 seconds
and the Main Menu will
Testing Menu
Editing Menu
Run PC Link
Main Menu
appear.
12:00:00 001/500
2. Press or to scroll
to the Editing Menu.
Testing Menu
Main Menu
Editing Menu
Run PC Link
12:00:00 001/500
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3. Press
ENTER
to select Editing
Menu. Press to scroll to
Edit User Test.
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
4. Press
ENTER
to select Edit
User Test.
5. Press or to scroll
to the desired user test.
6. Press
ENTER
to select the User
Test.
Edit User Test
105 USER TEST 01
106 USER TEST 02
107 USER TEST 03
108 USER TEST 04
12:00:00 001/500
Edit User Test
108 USER TEST 04
109 USER TEST 05
110 USER TEST 06
111 USER TEST 07
12:00:00 001/500
111 USER TEST 07
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
46 COD3 Plus Colorimeter 11.07
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NAMING THE TEST
A User Test can be up to 16 characters long. The menu choices for each
character are 26 upper case letters A to Z, 26 lower case letters a to z, ten
numerals 0 to 9, a space, a dash (-) and a decimal point (.). The existing name is
displayed on the bottom line of the display. The character which is to be edited
will blink and that character is also displayed in the center of the display. The
character can be changed by using or to scroll to other characters.
ENTER
Use
EXIT
to select a character. The edited name is saved at any time by pressing
or by pressing
ENTER
after selecting the sixteenth character.
1. From the User Test menu,
press to scroll to Name
the Test.
2. Press
ENTER
to select Name
the Test. A reverse font
(dark background with a
light character) will appear
to indicate the character that
will be adjusted. The same
character will also appear in
the center of the display.
3. Press or to scroll
to the desired character. In
this example, the test name
will be H2O.
111 USER TEST 07
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
Name the Test
R S T U V W X
111 U SER TEST 07
12:00:00 001/500
Name the Test
E F G H I J K
111 U SER TEST 07
12:00:00 001/500
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4. Press
ENTER
to save the
character and move to the
next character.
Name the Test
P Q R S T U V
111 H S ER TEST 07
12:00:00 001/500
5. Press or to scroll
to the desired character.
6. Press
ENTER
to save the
character and move to the
next character.
7. Press or to scroll
to the desired character.
Name the Test
. 0 1 2 3 4 5
111 H S ER TEST 07
12:00:00 001/500
Name the Test
L M N E P Q R
111 H2 E R TEST 07
12:00:00 001/500
Name the Test
L M N O P Q R
111 H2 O R TEST 07
12:00:00 001/500
48 COD3 Plus Colorimeter 11.07
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8. Press
ENTER
to save the
character. Repeat the
procedure until the test name
is complete. To remove
a character, change the
character to a space (located
after the letter z). Press
to save the name. The sreen
will display Storing... and the
test name for about 1 second
and the meter will return to
the Edit Test menu.
EXIT
111 H2O
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
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SELECTING THE VIAL AND WAVELENGTH
The COD3 Plus Colorimeter accepts three different vials (the 25 mm 0290
tube, UDVs and COD tubes) at 2 different wavelengths (428 and 635 nm). The
colorimeter uses different settings for each of the twelve combinations of vial and
wavelength. These twelve settings are called channels. Choose the channel with
the correct wavelength and vial for the test.
1. From the User Test menu,
press or to scroll
to Select Vial/WL.
2. Press
ENTER
to select Select
Vial/WL.
3. Press or to select
the channel with the desired
wavelingth and vial size
combination.
111 H2O
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
Select Channel
Ch1 428nm 25mm
Ch2 525nm 25mm
Ch3 635nm 25mm
Ch4 568nm 25mm
12:00:00 001/500
Select Channel
Ch1 428nm 25mm
Ch2 525nm 25mm
Ch3 635nm 25mm
Ch4 568nm 25mm
12:00:00 001/500
4. Press
ENTER
to select the
channel. The screen will
display Storing... for about
1 second and the meter will
return to the Edit Test menu.
Name the Test
Select Vial/WL
STD Calibration
111 H2O
Enter Constants
12:00:00 001/500
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ENTERING A TWO POINT CALIBRATION
The COD3 Plus Colorimeter can scan two reacted standards and create a
calibration curve. To prepare a calibration curve with multiple data points see
Entering a Multiple Calibration Curve (pg. 55).
1. From the User Test menu,
press or to
scroll to STD (Standard)
Calibration.
2. Press
ENTER
to select STD
Calibration. The screen will
display Low Standard for
about 1 second and then
display the Low Standard
screen. A reverse font (dark
background with a light
character) will appear to
indicate the character that
will be adjusted. The same
character will also appear in
the center of the display.
3. Press or to scroll
to the rst character of the
low concentration. In this
example, 1.00 ppm.
111 H2O
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
Low Standard
9 . – 0 1 2 3
0 0.000000
12:00:00 001/500
Low Standard
. – 0 1 1 2 3
0 .000000
12:00:00 001/500
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4. Press
ENTER
to save the
character and move to the
next character.
Low Standard
7 8 9 . – 0 1
1 . 000000
12:00:00 001/500
5. Press or to scroll
to the desired character.
6. Press
ENTER
to save the
character and move to the
next character.
7. Press or to scroll
to the desired character.
Low Standard
7 8 9 . – 0 1
1 . 000000
12:00:00 001/500
Low Standard
9 . – 0 1 2 3
1. 0 00000
12:00:00 001/500
Low Standard
9 . – 0 1 2 3
1. 0 00000
12:00:00 001/500
52 COD3 Plus Colorimeter 11.07
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8. Press
ENTER
to save the
character. Repeat the
procedure until the low
concentration value is
complete. After the nal
character is complete the
meter will save the low
concentration value. The
screen will display High
Standard for about 1 minute
and the meter will display
the High Standard screen. A
reverse font (dark background
with a light character)
will appear to indicate
the character that will be
adjusted. The same character
will appear in the center of the
display.
High Standard
9 . – 0 1 2 3
0 .0000
12:00:00 001/500
9. Use or and
to select the characters for the
high concentration value. In
this example, 7.5 ppm.
10. After the nal character is
entered the meter will save
the high concentration value.
The screen will display
instructions for completing the
calibration procedure.
ENTER
High Standard
9 . – 0 1 2 3
7.50000 0
12:00:00 001/500
Insert Blank
<Enter> continue
12:00:00 001/500
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11. Insert the blank. Press
ENTER
The screen will display Blank
Done... for about 1 second
and the Insert Low Standard
screen will appear.
12. Insert the low standard. Press
ENTER
. The screen will display
Reading... for about 1 second
and the Insert High Standard
screen will be displayed.
.
Insert Low Standard
<Enter> continue
12:00:00 001/500
Insert High Standard
<Enter> continue
12:00:00 001/500
13. Insert the high standard.
Press
ENTER
. The screen will
display Reading... for about
1 second and the meter will
return to the Edit Test menu.
111 H2O
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
54 COD3 Plus Colorimeter 11.07
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ENTERING A MULTIPLE POINT CALIBRATION
The COD3 Plus can directly create a 2 point calibration curve. (See Entering a
Two Point Calibration on page 51.) To create a multiple point calibration curve,
constants obtained from a linear regression of multiple data points can be
entered into the COD3 Plus.
1. Scan reactions of multiple concentrations at the appropriate wavelength in the
absorbance mode on the COD3 Plus.
2. Plot the concentration (y axis) versus absorbance (x axis) in a program
capable of linear regression such as Excel.
3. Enter the constants obtained from the linear regression equation into the
COD3 Plus.
For Example:
y = 0.001x3 – 0.017x2 + 0.181x – 0.049
K0 = – 0.049
K1 = 0.181
K2 = – 0.017
K3 = 0.001
OR (Over Range) = 10
1. From the User Test menu,
press or to scroll
to Enter Constants.
Name the Test
Select Vial/WL
111 H2O
STD Calibration
Enter Constants
12:00:00 001/500
2. Press
Constants.
ENTER
to select Enter
K0=0.00000
K1=0.00000
K2=0.00000
K3=0.00000
OR=100.00000
12:00:00 001/500
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3. Press
ENTER
to begin entering
the values for the constants. A
reverse font (dark background
with a light character)
will appear to indicate
the character that will be
adjusted. The same character
will also appear in the center
of the display.
Enter K0
9 . – 0 1 2 3
0 .00000
12:00:00 001/500
4. Press or to scroll
to the rst character of K0. In
this example, – 0.049.
5. Press
ENTER
to save the
character and move to the
next character.
6. Press or to scroll
to the next character.
Enter K0
8 9 . – 1 2 3
0 .00000
12:00:00 001/500
Enter K0
7 8 9 . – 0 1
– . 00000
12:00:00 001/500
Enter K0
9 . – O 1 2 3
– . 00000
12:00:00 001/500
56 COD3 Plus Colorimeter 11.07
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7. Press
ENTER
to save the
character and move to the
next character. Press
or to scroll to the next
character.
Enter K0
7 8 9 . – 0 1
– 0 0 0000
12:00:00 001/500
8. Press
ENTER
to save the
character. Repeat the
procedure until the K0 value
is complete. After the nal
character is complete the
meter will save the K0 value
and the meter will display K1
screen.
9. Use , and
ENTER
to select the characters for
the remaining values: K1, K2,
K3, and over range. In this
example, 10 ppm.
10. After the nal character is
entered the meter will save
the constants. The screen will
display Storing... and return
to the Edit Test menu.
Enter K1
9 . – 0 1 2 3
0 .00000
12:00:00 001/500
Enter Overrange
9 . – 0 1 2 3
10.00000 0
12:00:00 001/500
111 H2O
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
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SELECTING THE NUMERICAL FORMAT OF THE RESULT
To input tests with very different ranges, the number of decimal places displayed
for a result can be selected. A test which ranges from 20 to 1000 ppm should
not be displayed with three decimal places. A test with a range from 0.010 to
0.500 needs three decimal places (the microprocessor will always calculate the
concentration to many more signicant gures than will be displayed). The choice
of 0, 1, 2, or 3 decimal places are available.
1. From the User Test menu,
press or to scroll
to Decimal Places.
2. Press
ENTER
to select Decimal
Places.
3. Press or to scroll
to the desired number of
decimal places.
111 H2O
Select Vial/WL
STD Calibration
Enter Constants
Decimal Places
12:00:00 001/500
Decimal Places?
None 0
One 0.0
Two 0.00
Three 0.000
12:00:00 001/500
Decimal Places?
None 0
One 0.0
Two 0.00
Three 0.000
12:00:00 001/500
4. Press
ENTER
to select the
decimal places. The screen
wil display Storing... for about
1 second and the meter will
return to the Edit Test menu.
Select Vial/WL
STD Calibration
Enter Constants
111 H2O
Decimal Places
12:00:00 001/500
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SELECTING THE UNITS OF CONCENTRATION
The COD3 Plus Colorimeter has seven options for units of concentration. They
are No Units, ppm, FAU, pH, ppb, ppt and mgL.
1. From the User Menu, press
to scroll to Select
Units.
2. Press
ENTER
to select Select
Units.
3. Press or to scroll
to the desired units.
111 H2O
STD Calibration
Enter Constants
Decimal Places
Select Units
12:00:00 001/500
Select Units
No Units
ppm
pH
FAU
12:00:00 001/500
Select Units
No Units
ppm
pH
FAU
12:00:00 001/500
4. Press
ENTER
to select the
units. The screen will display
Storing... for about 1 second
and the meter will return to
the Edit Test menu.
STD Calibration
Enter Constants
Decimal Places
111 H2O
Select Units
12:00:00 001/500
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SETTING THE CLOCK
Setting the clock allows the correct time and date stamp to be stored with each
reading in the data logger.
1. From the Editing Menu, press
or to scroll to Set
Clock.
2. Press
ENTER
to select
Set Clock. The date is
displayed as month-dayyear. The time is displayed
as hours:minutes:seconds
AM/PM. Press or
to scroll to the appropriate
character. Press
ENTER
to
select the character. The
curser will move to the next
character. Set all characters
in the same manner. The
character menu is a scrolling
menu.
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
Set Time
Date: MM-DD-YYYY
Time: HH-MM-SS AM/PM
12:00:00 001/500
3. Press
ENTER
to select the nal
character. The time and date
will be saved and the meter
will return to the Edit Test
menu.
Edit Sequences
Edit User Test
Set Clock
Editing Menu
Logging
12:00:00 001/500
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LOGGING DATA
The default setting for the data logger is enabled. The meter will log the last 500
data points. The counter in the center bottom of the display will show how many
data points have been logged. The display will show 500+ when the data logger
has exceeded 500 points and the data points are being overwritten.
1. From the Editing Menu, press
or to scroll to
Logging.
2. Press
ENTER
to select
Logging.
3. Press or to scroll
to desired function.
Editing Menu
Edit Sequence
Edit User Test
Set Clock
Logging
12:00:00 001/500
Logging
Display Test Log
Enable Logging
Disable Logging
Erase Log
12:00:00 001/500
Logging
Display Test Log
Enable Logging
Disable Logging
Erase Log
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about
1 second and return to the
Logging menu.
Display Test Log
Enable Logging
Disable Logging
Logging
Erase Log
12:00:00 001/500
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5. Press
Editing Menu.
EXIT
to return to the
Edit Sequence
Editing Menu
Edit User Test
Set Clock
Logging
12:00:00 001/500
FACTORY SETUP
The Factory Setup menu is used in manufacturing of the COD3 Plus Colorimeter.
This menu is not for use by the operator in the eld.
SETTING POWER SAVE
The power saving Auto Shutoff feature will turn the meter off when a button has
not been pushed for a set amount of time. The default setting is disabled. To
change the setting:
1. From the Editing Menu, press
or to scroll to Set
PWR Save.
2. Press
ENTER
to select Set
PWR Save.
3. Press or to scroll
to desired function.
Editing Menu
Set Clock
Logging
Factory Setup
Set PWR Save
12:00:00 001/500
Auto Shutoff
Disable
5 Minutes
15 Minutes
30 Minutes
12:00:00 001/500
Auto Shutoff
Disable
5 Minutes
15 Minutes
30 Minutes
12:00:00 001/500
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4. Press
ENTER
. The screen will
display Storing... for about
1 second and the meter will
return to the Editing Menu.
Editing Menu
Set Clock
Logging
Factory Setup
Set PWR Save
12:00:00 001/500
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SETTING THE BACKLIGHT TIME
The backlight illuminates the display for enhanced viewing. The default setting is
10 seconds. If Button Control is chosen the backlight button on the key pad will
act as an on/off switch and the backlight will remain on or off when the meter is
being used. When one of the other settings – 10, 20 or 30 seconds – is chosen,
the display will be illuminated for the specied amount of time after any button is
pressed.
NOTE: The backlight feature uses a signicant amount of power. The longer the
backlight is on, the more frequently the battery will have to be charged if the
USB/Wall Adapter is not being used.
1. From the Editing Menu, press
or to scroll to
Backlight Time.
2. Press
ENTER
to select Set
Backlight Time.
3. Press or to scroll
to desired option.
Editing Menu
Logging
Factory Setup
Set PWR Save
Set Backlight Time
12:00:00 001/500
Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about
1 second and the meter will
return to the Editing Menu.
Logging
Factory Setup
Set PWR Save
Editing Menu
Set Backlight Time
12:00:00 001/500
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SELECTING A LANGUAGE
There are seven languages available in the COD3 Plus: English, Spanish, French,
Portuguese, Italian, Chinese, and Japanese.
1. From the Editing Menu, press
or to scroll to
Select Language.
2. Press
ENTER
to select Select
Language.
3. Press or to scroll
to desired language.
Editing Menu
Factory Setup
Set PWR Save
Set Backlight Time
Select Language
12:00:00 001/500
Select Language
English
Spanish
French
Portugese
12:00:00 001/500
Select Language
English
Spanish
French
Portugese
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about
1 second and the meter will
return to the Editing Menu.
Factory Setup
Set PWR Save
Set Backlight Time
Editing Menu
Select Language
12:00:00 001/500
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NOTE: If meter unintentionally switches to another language, use the procedure
above to reset the meter to the desired language. For example, to reset the meter
to English:
1. Turn meter on.
2. Press one time. Press
3. Press seven times. Press
4. Press
ENTER
.
ENTER
.
ENTER
.
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COMPUTER CONNECTION
PC LINK
The COD3 Plus may be interfaced with any Windows-based computer by using
the LaMotte SMARTLink 3 Program and USB Cable. The program will store
test information and results in a database. To transfer data from the meter to a
computer, plug the smaller end of the USB cable (USB mini B connector) into
the meter and the larger end of the USB cable (USB Type A connector) into a
USB port on a computer. The COD3 Plus will send the following data: test name,
wavelength, concentration, transmittance, absorbance, sample, blank, time of
test, and date of test.
OUTPUT
USB
COMPUTER CONNECTION
USB Type A, USB mini B, Order Cable Code 1720.
SMARTLINK3
SmartLink3 records the above data and appends a test ID# which uniquely
identies the test in the database, the serial number of the meter, and a site ID#
which can be used to associate the test record with a site or customer via the
SmartLink3 program. It also stores a “test number” which is useful for the COD3
Plus.
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BATTERY
BATTERY/AC OPERATION
The COD3 Plus may be operated on battery power, using a USB wall adapter or
USB computer connection. If using the meter as a bench top unit, use the wall
adapter if possible to extend the battery life. The meter will remain on when the
USB adapter is used.
To charge the battery with the wall adapter, plug the smaller end of the USB cable
(USB mini B connector) into the meter and the larger end of the USB cable (USB
Type A connector) into the wall adapter. Plug the wall adapter into an AC outlet.
Reinsert the USB port plug after charging.
To charge the battery from a computer, plug the smaller end of the USB cable
(USB mini B connector) into the meter and the larger end of the USB cable (USB
Type A connector) into a USB port on a computer.
The battery icon will show no bars and ash when the unit rst turns on. Then the
indicator will indicate the battery status by showing 0, 1, 2, 3 or 4 bars.
It will take 5 hours to fully charge a low battery. The battery icon will ash when
the battery is charging. The battery icon will show four bars and stop ashing
when it is fully charged. The charging circuit will automatically switch to a oat
charge when the battery is fully charged. The charger may remain connected.
Some computers will NOT supply power to their USB ports during standby
operation. The wall adapter will charge the unit continuously.
The battery icon will show no bars and continuously ash if the battery is getting
low but the unit will still operate normally. A “Low Battery” message on the
status bar of the display will replace the time when the battery voltage is too
low for proper operation and accuracy may be degraded. A “Shutdown Low
Batt” message on the display will appear for a few seconds before the power is
switched off when the battery is too low to operate the unit.
To extend the battery life:
• Shut down the unit with the power switch when not taking measurements
or use the power save option to have the unit automatically turn off after 5
minutes.
• Store the unit in a cool dry place.
• Fully charge the battery before storing the unit for extended periods of time.
• Limit backlight use. The unit consumes 3X normal power with the backlight
on. Set the backlight time option to 10 seconds, or select “Button Control”
and keep the backlight off.
Battery replacement: The lithium ion battery used in this unit should last for
many years with normal use. When it no longer powers the unit long enough to
meet testing requirements it will need to be replaced. Lithium ion batteries that
are properly charged and stored do not usually lose all capacity; they just have
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Page 69
less capacity after hundreds of charge cycles. This unit uses a custom battery
assembly that is only available from LaMotte Company. Battery replacement
must be performed at a LaMotte authorized repair facility. The water resistant
housing of this meter should not be opened by the user. Contact LaMotte
Company by phone (1-800-344-3100) or email (tech@lamotte.com) for a return
authorization number.
MAINTENANCE
CLEANING
Clean the exterior housing with a damp, lint-free cloth. Do not allow water to enter
the light chamber or any other parts of the meter. To clean the light chamber
and optics area, point a can of compressed air into the light chamber and blow
the pressurized air into the light chamber. Use a cotton swab dampened with
Windex® window cleaner to gently swab the interior of the chamber. Do not use
alcohol; it will leave a thin residue over the optics when dry.
REPAIRS
Should it be necessary to return the meter for repair or servicing, pack the
meter carefully in a suitable container with adequate packing material. A return
authorization number must be obtained from LaMotte Company by calling 800344-3100 (US only) or 410-778-3100, faxing 410-778-6394, or emailing tech@
lamotte.com. Often a problem can be resolved over the phone or by email. If
a return of the meter is necessary, attach a letter with the return authorization
number, meter serial number, a brief description of problem and contact
information including phone and FAX numbers to the shipping carton. This
information will enable the service department to make the required repairs more
efciently.
METER DISPOSAL
Waste Electrical and Electronic Equipment (WEEE)
Natural resources were used in the production of this equipment. This equipment
may contain materials that are hazardous to health and the environment. To avoid
harm to the environment and natural resources, the use of appropriate take-back
systems is recommended. The crossed out wheeled bin symbol on the meter
encourages the use of these systems when disposing of this equipment.
Take-back systems will allow the materials to be reused or recycled in a way that
will not harm the environment. For more information on approved collection,
reuse, and recycling systems contact local or regional waste administration or
recycling services.
COD3 Plus Colorimeter 11.14 69
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Page 70
TROUBLESHOOTING
ERROR MESSAGES
OVER RANGE
If the message OVERRANGE is displayed when scanning a sample, the sample
may be over range or under range. If the sample is over range the sample should
be diluted and tested again (see Sample Dilution Techniques and Volumetric
Measurements, page 19).
If overrange is displayed, press
ENTER
to continue testing on
diluted samples.
Note: After pressing
ENTER
, the
overrange cncentration will be
displayed. This concentration is
an approximation only.
Overrange
<Enter> continue
Scan Blank
Scan Sample
12:00:00 001/500
002 Aluminum
CALIBRATION
As with all pre-calibrated meters, it is highly recommended, even if not required
by regulations, that the user periodically verify the performance of the meter
by running standards with a predetermined concentration. Results outside of
specication are an indication that the meter needs to be adjusted. This can be
done following the user calibration described on page 28. If the user calibration
fails to properly adjust the meter then the meter should be returned to LaMotte
Company for recalibration. (See page 65).
STRAY LIGHT
The COD3 Plus Colorimeter should have no problems with stray light. Make sure
that the sample compartment lid is always fully closed, except when testing COD
with the adapter.
70 COD3 Plus Colorimeter 11.07
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Page 71
TROUBLESHOOTING GUIDE
PROBLEM REASON SOLUTION
Flashing
Low battery. Readings
are reliable.
“Low Battery” Battery voltage is very
low. Readings are not
reliable.
“Shut Down Low
Batt” Shut Down
Battery is too low to
operate the unit.
“Overrange” Sample is outside of
acceptable range.
Charge battery or use
USB wall/computer
adapter.
Charge battery or use
USB wall/computer
adapter.
Charge battery or use
USB wall/computer
adapter.
Dilute sample and test
again.
Unusually large
negative or
positive readings
when performing
calibration
Incorrect standards
used to calibrate
meter.
Use fresh 0.0 standard
in clean tube. Reset
meter to factory default
settings. Recalibrate
meter.
COD3 Plus Colorimeter 11.14 71
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Page 72
72 COD3 Plus Colorimeter 11.07
www.GlobalTestSupply.com
Page 73
1925-TEST
Version 1.0
3.13.11
COD3 Plus Colorimeter 11.14 73
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Page 74
COD3 Plus COLORIMETER
REAGENT SYSTEMS
COD3 Plus REAGENT SYSTEMS LIST
LaMotte Company continuously updates the list of pre-programmed tests as the
calibrations become available. Pre-programmed calibrations can be added to
the COD3 Plus Colorimeter in the eld. A Windows-based computer running a
Windows Operating System and an 8 pin mini-DIN/9 pin F D-submin serial cable
(order Code 1771) are required.
Range
Test Factor (Test #)
COD-Low Range (001) 0–150 7.5 Digestion (1) 25
COD-Standard Range (002) 0-1500 40 Digestion (1) 25
COD-High Range (003) 0–15000 400 Digestion (1) 25
Ammonia Nitrogen-
Low Range, Fresh Water (005)
Ammonia Nitrogen-
Low Range, Salt Water (004)
Ammonia Nitrogen-
High Range (006)
Boron (007) 0.00-0.80 0.05 Azomethine-H (2) 50
Cobalt (008) 0.00–2.00 0.04 PAN (3) 50
Color (009) 0–1000 20 Platinum Cobalt (0) –
Copper-Cuprizone (010) 0.00–2.50 0.03 Cuprizone (2) 50
Copper-DDC (011) 0.00–7.00 0.10 Diethyldithiocarbamate (1) 50
Cyanuric Acid (012) 5–200 10 Melamine (1) 50
Dissolved Oxygen (013) 0.0–10.0 0.6 Winkler Colorimetric (3) 100
Fluoride (014) 0.00–2.00 0.10 SPADNS (2) 50
Hydrazine (015) 0.00–1.00 0.01 P-dimethyl-
Molybdenum-High Range (016) 0.0–50.0 0.6 Thioglycolate (3) 50
Nickel (017) 0.00–8.00 0.15 Dimethylglyoxime (6) 50
Ozone-Low Range (018) 0.00–0.40 0.02 Indigo Trisulfonate (3) 100
Ozone-High Range (019) 0.00–3.00 0.05 Indigo Trisulfonate (3) 20
Phosphate-Low Range (020) 0.00–3.00 0.05 Ascorbic Acid Reduction (2) 25
Phosphate-High Range (021) 0.0–70.0 0.5 Vanodomolybd-
(ppm) MDL
0.00–1.00 0.05 Salicylate (3) 25
0.00–1.00 0.10 Salicylate (3) 25
0.00–4.00 0.05 Nesslerization (2) 50
Test Method (# of
Reagents)
aminobenzaldehyde (2)
phosphoric Acid (1)
# of
Tests
50
25
74 COD3 Plus Colorimeter 11.07
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Page 75
Potassium (022) 0.0-10.0 0.8 Tetraphenylboron (2) 100
Silica-Low Range (023) 0.0–4.0 0.05 Heteropoly Blue (4) 50
Silica-High Range (024) 0–75 0.5 Silicomolybdate (3) 50
Sulfate-High Range (025) 0–100 3 Barium Chloride (1) 50
Sulde-Low Range (026) 0.00–1.50 0.06 Methylene Blue (3) 50
Tannin (027) 0.0–10.0 0.1 Tungsto-molybdophosphoric
Acid (2)
Turbidity (028) 0.0–30.0
FTU
Zinc-Low Range (029) 0.00–3.00 0.05 Zincon (6) 50
3 Absorption (0) –
50
www.GlobalTestSupply.com
Page 76
Test Procedures
www.GlobalTestSupply.com
Page 77
COD – LOW RANGE
MERCURY FREE DIGESTION METHOD • CODE 0072-SC
MERCURY DIGESTION METHOD • CODE 0075-SC
QUANTITY CONTENTS CODE
25 *COD Low Range Mercury Free Tubes *0072-SC
or 25 *COD Low Range Mercury Tubes *0075-SC
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
COD Low Range Mercury Free Tubes are not USEPA approved.
COD Low Range Mercury Tubes are USEPA approved.
Equipment needed but not supplied:
1 COD Adapter 5-0087
1 COD Reactor, 12 tube, 110V 5-0102
or 1 COD Reactor, 12 tube, 230V 5-0102-EX2
1 Measuring Pipet, 1.0 mL 2-2110
1 Pipet Bulb 2-2164
Test Procedures
Chemical Oxygen Demand (COD) is a measure of the amount of organic matter
in water which is susceptible to oxidation by chemical oxidants. COD can be
empirically related to the Biological Oxygen Demand (BOD) and organic carbon
content of a specic source of water. This correlation must be determined
experimentally for each source of water.
COD3 Plus Colorimeter 3.11 COD, Low Range
www.GlobalTestSupply.com
Page 78
APPLICATION: Domestic and industrial wastes.
RANGE: 0–150 mg/L COD
MDL: 7.5 mg/L
METHOD: Dichromate in the presence of silver salts, at high
temperature in a closed system, oxidizes most organic
compounds to 95-100% of the theoretical amount. This
process is called digestion. As dichromate oxidizes
the organic compounds, the amount of yellow color
is reduced. The remaining yellow color is measured
colorimetrically at the 420 nm and is directly proportional
to the COD of the sample.
SAMPLE
HANDLING &
PRESERVATION:
Collect samples in glass and test as soon as possible. If
samples must be stored, preservation is accomplished
by the addition of concentrated H2SO4 to adjust the
pH below 2. Samples with suspended solids should be
homogenized in a blender (100 mL for 30 seconds) and
then stirred gently with a magnetic stirrer.
INTERFERENCES: Volatile organic compounds are not oxidized to the
extent that they are in the vapor above the digestion
solution. Therefore, they do not contribute to the
COD reading. Chloride concentrations above 10% of
COD interfere with the mercury free tubes. Chloride
above 2000 ppm will interfere with the mercury tubes.
Nitrite gives a positive interference of 1.1 ppm O
per ppm NO2–N which is insignicant unless nitrite
concentrations are very high. Other reduced inorganic
compounds are stoichiometrically oxidized, causing
a positive interference. Corrections can be made for
these compounds based upon their stoichiometry and
Test Procedures
concentrations.
2
When scanning samples in 16 mm tubes, such as COD,
the sample chamber lid can not be closed. Use the COD
adapter to minimize stray light interference. To further
reduce stray light interference, do not scan sample in
direct sunlight.
COD, Low Range COD3 Plus Colorimeter 3.11
www.GlobalTestSupply.com
Page 79
PROCEDURE
Use COD/UDV adapter.
1. Homogenize sample if necessary.
2. Preheat COD heater block to 150±2°C.
3. Remove cap from COD tube. Hold tube at a 45° angle. Use a volumetric
pipet, to carefully add 2.0 mL sample water allowing the sample to run down
the side of the tube.
4. Cap and mix thoroughly.
5. Rinse the outside of the tube with distilled water. Wipe dry with a paper
towel.
6. Repeat steps 3 through 5 using 2.0 mL distilled water. This is the reagent
blank.
7. Place tubes in preheated COD block heater and maintain temperature at
150±2°C for two hours.
8. At the end of the heating period turn the heater off. Wait 20 minutes for the
tubes to cool to 120°C or less.
9. Remove tubes from block heater. Invert several times to mix.
10. Allow to cool to room temperature.
11. Press and hold until colorimeter turns on.
12. Press
ENTER
to select TESTING MENU.
13. Select ALL TESTS (or another sequence containing 001 COD LR) from
PROGRAMMED TESTS menu.
14. Scroll to and select 001 COD LR from menu.
15. Wipe the blank tube with a damp towel to remove ngerprints and smudges.
Wipe with a dry towel.
16. Insert reagent blank tube into chamber. Select SCAN BLANK.
17. Remove tube from colorimeter.
18. Insert digested water sample tube into chamber. Select SCAN SAMPLE.
Record result. For the most accurate results, take three readings on each
sample and average the results.
19. Press to turn colorimeter off or press
EXIT
to exit to a previous menu
or make another menu selection.
NOTES: Reagents are light sensitive. Unused reagents should be stored in the
shipping container, and in the refrigerator if possible, until needed.
A reagent blank should be run with each set of samples and with each lot of
reagents.
The reacted blank will be stable if stored in the dark.
COD3 Plus Colorimeter 3.11 COD, Low Range
www.GlobalTestSupply.com
Test Procedures
Page 80
To eliminate error caused by contamination, wash all glassware with 20% sulfuric
acid.
For greater accuracy, a minimum of three repetitions should be performed and
the results averaged.
Some samples may be digested completely in less than two hours. The
concentration may be measured at 15 minute intervals while the vials are still
hot until the reading remains unchanged. The vials should be cooled to room
temperature before the nal measurement is taken.
Test Procedures
COD, Low Range COD3 Plus Colorimeter 3.11
www.GlobalTestSupply.com
Page 81
COD – STANDARD RANGE
MERCURY FREE DIGESTION METHOD • CODE 0073-SC
MERCURY DIGESTION METHOD • CODE 0076-SC
QUANTITY CONTENTS CODE
25 *COD Standard Range Mercury Free Tubes *0073-SC
or 25 *COD Standard Range Mercury Tubes *0076-SC
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
COD Standard Range Mercury Free Tubes are not USEPA approved.
COD Standard Range Mercury Tubes are USEPA approved.
Equipment needed but not supplied:
1 COD Adapter 5-0087
1 COD Reactor, 12 tube, 110V 5-0102
or 1 COD Reactor, 12 tube, 230V 5-0102-EX2
1 Measuring Pipet, 1.0 mL 2-2110
1 Pipet Bulb 2-2164
Test Procedures
Chemical Oxygen Demand (COD) is a measure of the amount of organic matter
in water which is susceptible to oxidation by chemical oxidants. COD can be
empirically related to the Biological Oxygen Demand (BOD) and organic carbon
content of a specic source of water. This correlation must be determined
experimentally for each source of water.
COD3 Plus Colorimeter 3.11 COD, Standard Range
www.GlobalTestSupply.com
Page 82
APPLICATION: Domestic and industrial wastes.
RANGE: 0–1500 mg/L COD
MDL: 40 mg/L
METHOD: Dichromate in the presence of silver salts, at high
temperature in a closed system, oxidizes most
organic compounds to 95-100% of the theoretical
amount. This process is called digestion. As
dichromate oxidizes the organic compounds, a
green complex is formed. The concentration of the
green complex is measured at 605 nm and is directly
proportional to the COD of the sample.
SAMPLE HANDLING &
PRESERVATION:
Collect samples in glass and test as soon as
possible. If samples must be stored, preservation is
accomplished by the addition of concentrated H
to adjust the pH below 2. Samples with suspended
solids should be homogenized in a blender (100
mL for 30 seconds) and then stirred gently with a
magnetic stirrer.
INTERFERENCES: Volatile organic compounds are not oxidized to the
extent that they are in the vapor above the digestion
solution. Therefore, they do not contribute to the
COD reading. Chloride concentrations above 10% of
COD interfere with the mercury free tubes. Chloride
above 2000 ppm will interfere with the mercury
tubes. Nitrite gives a positive interference of 1.1 ppm
per ppm NO2–N which is insignicant unless
O
2
nitrite concentrations are very high. Other reduced
inorganic compounds are stoichiometrically oxidized,
causing a positive interference. Corrections can
Test Procedures
be made for these compounds based upon their
stoichiometry and concentrations.
2SO4
When scanning samples in 16 mm tubes, such as
COD, the sample chamber lid can not be closed. Use
the COD adapter to minimize stray light interference.
To further reduce stray light interference, do not scan
sample in direct sunlight.
COD, Standard Range COD3 Plus Colorimeter 3.11
www.GlobalTestSupply.com
Page 83
PROCEDURE
Use COD/UDV adapter.
1. Homogenize sample if necessary.
2. Preheat COD heater block to 150±2°C.
3. Remove cap from COD tube. Hold tube at a 45° angle. Use a volumetric
pipet, to carefully add 2.0 mL sample water allowing the sample to run down
the side of the tube.
4. Cap and mix thoroughly.
5. Rinse the outside of the vial with distilled water. Wipe dry with a paper
towel.
6. Repeat steps 2 through 5 using 2.0 mL distilled water. This is the reagent
blank.
7. Place tubes in preheated COD block heater and maintain temperature at
150±2°C for two hours.
8. At the end of the heating period turn the heater off. Wait 20 minutes for the
tubes to cool to 120°C or less.
9. Remove tubes from block heater. Invert several times to mix.
10. Allow to cool to room temperature.
11. Press and hold until colorimeter turns on.
12. Press
ENTER
to select TESTING MENU.
13. Select ALL TESTS (or another sequence containing 002 COD SR) from
TESTING MENU menu.
14. Wipe the blank tube with a damp towel to remove ngerprints and smudges.
Wipe with a dry towel.
15. Scroll to and select 002 COD SR from menu.
16. Insert reagent blank tube into chamber. Select SCAN BLANK.
17. Remove tube from colorimeter.
18. Insert digested water sample tube into chamber. Select SCAN SAMPLE.
Record result. For the most accurate results, take three readings on each
sample and average the results.
19. Press to turn colorimeter off or press
EXIT
to exit to a previous menu
or make another menu selection.
NOTES: Reagents are light sensitive. Unused reagents should be stored in the
shipping container, and in the refrigerator if possible, until needed.
A reagent blank should be run with each set of samples and with each lot of
reagents.
The reacted blank will be stable if stored in the dark.
COD3 Plus Colorimeter 3.11 COD, Standard Range
www.GlobalTestSupply.com
Test Procedures
Page 84
To eliminate error caused by contamination, wash all glassware with 20% sulfuric
acid.
For greater accuracy, a minimum of three repetitions should be performed and
the results averaged.
Some samples may be digested completely in less than two hours. The
concentration may be measured at 15 minute intervals while the vials are still
hot until the reading remains unchanged. The vials should be cooled to room
temperature before the nal measurement is taken.
Test Procedures
COD, Standard Range COD3 Plus Colorimeter 3.11
www.GlobalTestSupply.com
Page 85
COD – HIGH RANGE
MERCURY FREE DIGESTION METHOD • CODE 0074-SC
MERCURY DIGESTION METHOD • CODE 0077-SC
QUANTITY CONTENTS CODE
25 *COD High Range Mercury Free Tubes *0074-SC
or 25 *COD High Range Mercury Tubes *0077-SC
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
COD High Range Mercury Free Tubes and COD High Range Mercury Tubes are
not USEPA approved.
Equipment needed but not supplied:
1 COD Adapter 5-0087
1 COD Reactor, 12 tube, 110V 5-0102
or 1 COD Reactor, 12 tube, 230V 5-0102-EX2
1 Measuring Pipet, 1.0 mL 2-2110
1 Pipet Bulb 2-2164
Chemical Oxygen Demand (COD) is a measure of the amount of organic matter
in water which is susceptible to oxidation by chemical oxidants. COD can be
empirically related to the Biological Oxygen Demand (BOD) and organic carbon
content of a specic source of water. This correlation must be determined
experimentally for each source of water.
Test Procedures
COD3 Plus Colorimeter 3.11 COD, High Range
www.GlobalTestSupply.com
Page 86
APPLICATION: Domestic and industrial wastes.
RANGE: 0–15000 mg/L COD
MDL: 400 mg/L
METHOD: Dichromate in the presence of silver salts, at high
temperature in a closed system, oxidizes most organic
compounds to 95-100% of the theoretical amount. This
process is called digestion. As dichromate oxidizes the
organic compounds, a green complex is formed. The
concentration of the green complex is measured at
605 nm and is directly proportional to the COD of the
sample.
SAMPLE
HANDLING &
RESERVATION:
Collect samples in glass and test as soon as possible. If
samples must be stored, preservation is accomplished
by the addition of concentrated H2SO4 to adjust the
pH below 2. Samples with suspended solids should be
homogenized in a blender (100 mL for 30 seconds) and
then stirred gently with a magnetic stirrer.
INTERFERENCES: Volatile organic compounds are not oxidized to the
extent that they are in the vapor above the digestion
solution. Therefore, they do not contribute to the
COD reading. Contains mercury sulfate to prevent
interference from chloride. Nitrite gives a positive
interference of 1.1 ppm O
per ppm NO2–N, which
2
is insignicant unless nitrite concentrations are
very high. Other reduced inorganic compounds
are stoichiometrically oxidized, causing a positive
interference. Corrections can be made for these
compounds based upon their stoichiometry and
concentrations.
Test Procedures
When scanning samples in 16 mm tubes, such as COD,
the sample chamber lid can not be closed. Use the COD
adapter to minimize stray light interference. To further
reduce stray light interference, do not scan sample in
direct sunlight.
COD, High Range COD3 Plus Colorimeter 3.11
www.GlobalTestSupply.com
Page 87
PROCEDURE
Use COD/UDV adapter.
1. Homogenize sample if necessary.
2. Preheat COD heater block to 150±2°C.
3. Remove cap from COD tube. Hold tube at a 45° angle. Use a graduated
pipet, to carefully add 0.2 mL sample water allowing the sample to run down
the side of the tube.
4. Cap and mix thoroughly.
5. Rinse the outside of the tube with distilled water. Wipe dry with a paper
towel.
6. Repeat steps 3 through 5 using 0.2 mL distilled water. This is the reagent
blank.
7. Place tubes in preheated COD block heater and maintain temperature at
150±2°C for two hours.
8. At the end of the heating period turn the heater off. Wait 20 minutes for the
tubes to cool to 120°C or less.
9. Remove tubes from block heater. Invert several times to mix.
10. Allow to cool to room temperature.
11. Press and hold until colorimeter turns on.
12. Press
ENTER
to select TESTING MENU.
13. Select ALL TESTS (or another sequence containing 003 COD HR) from
TESTING MENU menu.
14. Wipe the blank tube with a damp towel to remove ngerprints and smudges.
Wipe with a dry towel.
15. Scroll to and select 003 COD HR from menu.
16. Insert reagent blank tube into chamber. Select SCAN BLANK.
17. Remove tube from colorimeter.
18. Insert digested water sample tube into chamber. Select SCAN SAMPLE.
Record result. For the most accurate results, take three readings on each
sample and average the results.
19. Press to turn colorimeter off or press
EXIT
to exit to a previous menu
or make another menu selection.
NOTES: Reagents are light sensitive. Unused reagents should be stored in the
shipping container, and in the refrigerator if possible, until needed.
A reagent blank should be run with each set of samples and with each lot of
reagents.
The reacted blank will be stable if stored in the dark.
COD3 Plus Colorimeter 3.11 COD, High Range
www.GlobalTestSupply.com
Test Procedures
Page 88
To eliminate error caused by contamination, wash all glassware with 20% sulfuric
acid.
For greater accuracy, a minimum of three repetitions should be performed and
the results averaged.
Test Procedures
COD, High Range COD3 Plus Colorimeter 3.11
www.GlobalTestSupply.com
Page 89
AMMONIA NITROGEN - LOW RANGE
SALICYLATE METHOD • CODE 3659-01-SC
QUANTITY CONTENTS CODE
60 mL *Salicylate Ammonia #1 *3978-H
10 g *Salicylate #2 *7457-D
2 x 5 g *Salicylate #3 Reagent Powder *7458-C
1 Spoon, 0.1 g, plastic 0699
1 Spoon, 0.15 g, plastic 0727
1 Pipet, 1.0 mL, plastic 0354
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
Ammonia nitrogen is present in various concentrations in many surface and
ground water supplies. Any sudden change in the concentration of ammonia
nitrogen in a water supply is cause for suspicion. A product of microbiological
activity, ammonia nitrogen is sometimes accepted as chemical evidence of
pollution when encountered in natural waters.
Ammonia is rapidly oxidized in natural water systems by special bacterial groups
that produce nitrite and nitrate. This oxidation requires that dissolved oxygen be
available in the water. Ammonia is an additional source of nitrogen as a nutrient
which may contribute to the expanded growth of undesirable algae and other
forms of plant growth that overload the natural system and cause pollution.
Test Procedures
COD3 Plus Colorimeter 3.11 AMMONIA NITROGEN, Low Range
www.GlobalTestSupply.com
Page 90
APPLICATION: Low concentrations of ammonia in fresh, brackish and
salt water; fresh and salt water aquariums.
RANGE: 0.00 - 1.00 ppm Ammonia-Nitrogen
MDL: 0.05 ppm Fresh Water
0.10 ppm Salt Water
METHOD: Salicylate and ammonia react at high pH in the presence
of a chlorine donor and an iron catalyst to form a
blue indophenol dye, the concentration of which is
proportional to the ammonia concentration in the sample.
SAMPLE HANDLE
& PRESERVATION:
Ammonia solutions tend to be unstable and should be
analyzed immediately. Samples may be stored for 24
hours at 4°C or 28 days at –20°C.
INTERFERENCES: There are few interferences in most natural waters. High
concentrations of reducing agents, such as hydrazine,
react with the chlorine donor and can result in negative
interferences. Color and turbidity can also interfere.
Test Procedures
AMMONIA NITROGEN, Low Range COD3 Plus Colorimeter 3.11
www.GlobalTestSupply.com
Page 91
PROCEDURE - FRESH WATER
1. Press and hold until colorimeter turns on.
2. Press
3. Select ALL TESTS (or another sequence containing 004 Ammonia-N LRF)
4. Scroll to and select 004 Ammonia-N LRF from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with
6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.)
7. Remove tube from colorimeter. Use the 1.0 mL plastic pipet (0354) to add 2.0
8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent
9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two
10. At the end of the 12 minute waiting period, immediately mix and insert tube
11. Press to turn colorimeter off or press
CALCULATIONS:
ENTER
to select TESTING MENU.
from TESTING MENU.
sample.
mL of *Salicylate Ammonia #1 (3978). Cap and mix.
(7457). Cap and mix until dissolved. Wait 1 minute.
measures of *Salicylate #3 Reagent Powder (7458). Cap and shake
vigorously for at least 30 seconds and all solid has dissolved. Wait 12 minutes
for maximum color development.
into chamber, close lid and select SCAN SAMPLE. Record result.
EXIT
to exit to a previous menu
or make another menu selection.
Test Procedures
To express results as Unionized Ammonia (NH3):
ppm Unionized Ammonia (NH3) =
ppm Ammonia-Nitrogen (NH3–N) x 1.2
To express results as Ionized Ammonia (NH4):
ppm Ionized Ammonia (NH
4
+
) =
ppm Ammonia-Nitrogen (NH3–N) x 1.3
To determine the percentages of Unionized and Ionized Ammonia-Nitrogen,
consult the Appendix.
NOTE: It is strongly suggested that a reagent blank be determined to account
for any contribution to the test result by the reagent system. To determine the
reagent blank, follow the above test procedure to scan a distilled or deionized
water blank. Then follow the above procedure to perform the test on a distilled or
deionized water sample. This test result is the reagent blank. Subtract the reagent
blank from all subsequent test results of unknown samples. It is necessary to
determine the reagent blank only when a new lot number of reagents is obtained.
COD3 Plus Colorimeter 3.11 AMMONIA NITROGEN, Low Range
www.GlobalTestSupply.com
Page 92
PROCEDURE - SALT WATER
1. Press and hold until colorimeter turns on.
2. Press
3. Select ALL TESTS (or another sequence containing 005 Ammonia-N LRS)
4. Scroll to and select 005 Ammonia-N LRS from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with
6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.)
7. Remove tube from colorimeter. Use the 1.0 mL plastic pipet (0354) to add 2.0
8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent
9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two
10. At the end of the 20 minute waiting period, immediately mix and insert tube
11. Press to turn colorimeter off or press
CALCULATIONS:
ENTER
to select TESTING MENU.
from TESTING MENU.
sample.
mL of *Salicylate Ammonia #1 (3978). Cap and mix.
(7457). Cap and mix until dissolved. Wait 1 minute.
measures of *Salicylate #3 Reagent Powder (7458). Cap and shake
vigorously for at least 30 seconds and all solid has dissolved. Wait 20 minutes
for maximum color development.
into chamber, close lid and select SCAN SAMPLE. Record result.
EXIT
to exit to a previous menu
or make another menu selection.
To express results as Unionized Ammonia (NH3):
ppm Unionized Ammonia (NH3) =
Test Procedures
ppm Ammonia-Nitrogen (NH3–N) x 1.2
To express results as Ionized Ammonia (NH4):
ppm Ionized Ammonia (NH
+
4
) =
ppm Ammonia-Nitrogen (NH3–N) x 1.3
To determine the percentages of Unionized and Ionized Ammonia-Nitrogen,
consult the Appendix.
NOTE: It is strongly suggested that a reagent blank be determined to account
for any contribution to the test result by the reagent system. To determine the
reagent blank, follow the above test procedure to scan a distilled or deionized
water blank. Then follow the above procedure to perform the test on a distilled or
deionized water sample. This test result is the reagent blank. Subtract the reagent
blank from all subsequent test results of unknown samples. It is necessary to
determine the reagent blank only when a new lot number of reagents is obtained.
AMMONIA NITROGEN, Low Range COD3 Plus Colorimeter 3.11
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Page 93
AMMONIA NITROGEN - HIGH RANGE
NESSLERIZATION METHOD •CODE 3642-SC
QUANTITY CONTENTS CODE
30 mL Ammonia Nitrogen Reagent #1 V-4797-G
2 x 30 mL *Ammonia Nitrogen Reagent #2 *V-4798-G
1 Pipet, 1 mL, plastic 0354
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
Ammonia nitrogen is present in various concentrations in many surface and
ground water supplies. Any sudden change in the concentration of ammonia
nitrogen in a water supply is cause for suspicion. A product of microbiological
activity, ammonia nitrogen is sometimes accepted as chemical evidence of
pollution when encountered in natural waters.
Ammonia is rapidly oxidized in natural water systems by special bacterial groups
that produce nitrite and nitrate. This oxidation requires that dissolved oxygen be
available in the water. Ammonia is an additional source of nitrogen as a nutrient
which may contribute to the expanded growth of undesirable algae and other
forms of plant growth that overload the natural system and cause pollution.
APPLICATION: Drinking, surface, and saline waters; domestic and
industrial wastes.
RANGE: 0.00–4.00 ppm Ammonia Nitrogen
MDL: 0.05 ppm
METHOD: Ammonia forms a colored complex with Nessler’s
Reagent in proportion to the amount of ammonia
present in the sample. Rochelle salt is added to
prevent precipitation of calcium or magnesium in
undistilled samples.
SAMPLE HANDLING &
PRESERVATION:
INTERFERENCES: Sample turbidity and color may interfere. Turbidity
Ammonia solutions tend to be unstable and should
be analyzed immediately. Sample may be stored for
24 hours at 4°C or 28 days at –20°C.
may be removed by a ltration procedure. Color
interference may be eliminated by blanking the
instrument with a sample blank.
Test Procedures
COD3 Plus Colorimeter 3.11 AMMONIA NITROGEN, High Range
www.GlobalTestSupply.com
Page 94
PROCEDURE
1. Press and hold until colorimeter turns on.
2. Press
3. Scroll to and select ALL TESTS (or another sequence containing 006
4. Scroll to and select 006 Ammonia-N HR from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with
6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note)
7. Remove tube from colorimeter. Add 8 drops of Ammonia Nitrogen Reagent
8. Use the 1.0 mL pipet (0354) to add 1.0 mL of *Ammonia Nitrogen Reagent #2
9. At end of the 5 minute waiting period, immediately mix, insert tube into
10. Press to turn the colorimeter off or press the
CALCULATIONS:
To express results as Unionized Ammonia (NH3):
ENTER
to select TESTING MENU.
Ammonia-N HR) from TESTING MENU.
sample.
#1 (V-4797). Cap and mix. Wait 1 minute.
(V-4798). Cap and mix. Allow 5 minutes for maximum color development.
chamber, close lid and select SCAN SAMPLE. Record result.
EXIT
exit to a previous
menu or make another menu selection.
ppm Unionized Ammonia (NH3) =
ppm Ammonia-Nitrogen (NH3–N) x 1.2
To express results as Ionized Ammonia (NH4):
ppm Ionized Ammonia (NH
+
4
) =
ppm Ammonia-Nitrogen (NH3–N) x 1.3
Test Procedures
To determine the percentages of Unionized and Ionized Ammonia-Nitrogen,
consult the Appendix.
NOTE: It is strongly suggested that a reagent blank be determined to account
for any contribution to the test result by the reagent system. To determine the
reagent blank, follow the above test procedure to scan a distilled or deionized
water blank. Then follow the above procedure to perform the test on a distilled or
deionized water sample. This test result is the reagent blank. Subtract the reagent
blank from all subsequent test results of unknown samples. It is necessary to
determine the reagent blank only when a new lot number of reagents is obtained.
AMMONIA NITROGEN, High Range COD3 Plus Colorimeter 3.11
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Page 95
BORON
AZOMETHINE-H METHOD · CODE 4868-01
QUANTITY CONTENTS CODE
120 mL *Boron Buffer *4869-J
10 g *Boron Indicator Powder *4870-D
1 Pipet, plastic, 1.0 mL 0354
1 Spoon, 0.15 g 0727
1 Dark storage chamber, brown 0108
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
Small amounts of boron are necessary for plant growth but large amounts can
be toxic. In humans, boron aids in the uptake of calcium and the production
of strong bones. An excess of boron can affect the central nervous system
resulting in a syndrome known as borism. Some natural waters may contain small
amounts of boron. Large concentrations may be due to industrial efuent entering
waterways. Boron compounds are used in cleaning compounds, paper and
paints, fertilizers, glass and ceramics, re retardants and the production of alloys.
In the atomic energy eld, boron is a component of neutron shields and nuclear
reactors. Some swimming pools use boron buffering systems.
Test Procedures
APPLICATION: Surface and saline waters, hydroponic solutions,
industrial waste, swimming pools.
RANGE: 0.00–0.80 ppm Boron
MDL: 0.05
METHOD: Azomethine-H and borate form a yellow complex at pH
6 in proportion to the concentration of boron present.
SAMPLE HANDLING
& PRESERVATION:
INTERFERENCES: Interferences in drinking water are unlikely. Manganese,
COD3 Plus Colorimeter 3.11 BORON
Store samples in polyethylene bottles. Do not use
borate detergents or glassware.
zirconium, chromium, titanium, copper, vanadium,
aluminum, beryllium and iron may cause high results.
www.GlobalTestSupply.com
Page 96
PROCEDURE
1. This test requires a Reagent Blank. Rinse a tube (0290) with clear, colorless,
boron free water. Fill to 10 mL line with clear, colorless, boron free water.
2. Use the 1.0 mL pipet (0354) to add 2 mL of *Boron Buffer (4869). Cap and
mix.
3. Use the 0.15 g spoon (0727) to add one level measure of *Boron Indicator
Powder (4870). Press full spoon against side of jar to compress powder.
Scrape off excess powder on inside neck of bottle. Tap excess off spoon
handle.
4. Cap and shake vigorously for 30 seconds.
5. Insert the tube into meter chamber. Close lid.
6. Start a timer set for 30 minutes. Do not open the lid during the waiting time.
The reaction is photosensitive.
7. Rinse a clean tube (0290) with Sample Water. Fill to the 10 mL line with
sample water. Repeat steps 2–4.
8. Insert the tube into the Dark Storage Chamber (0108). Close top.
9. Start a second timer set for 30 minutes. Do not open the chamber during the
waiting time. The reaction is photosensitive.
10. When 2 minutes remain on the rst timer (Reagent Blank), press and hold ON
button until colorimeter turns on.
11. Press and hold until colorimeter turns on.
12. Press
13. Select ALL TESTS (or another sequence containing 007 Boron) from
14. Scroll to and select 007 Boron from menu.At the end of the Reagent Blank
Test Procedures
15. Insert the tube into meter chamber, close lid and select SCAN BLANK.
16. Remove the tube from colorimeter.
17. At the end of the Sample Water 30 minute waiting period, remove Sample
18. Insert tube into meter chamber, close lid and select SCAN SAMPLE. Record
19. Press to turn colorimeter off or press the
ENTER
to select TESTING MENU.
TESTING MENU.
30 minute waiting period, remove Reagent Blank tube from meter chamber.
Invert several times to mix.
Water tube from Dark Storage Chamber. Invert several times to mix.
result in ppm boron.
EXIT
to exit to a previous
menu or make another menu selection.
BORON COD3 Plus Colorimeter 3.11
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Page 97
COBALT
PAN METHOD · CODE 4851
QUANTITY CONTENTS CODE
60 mL *Cobalt Buffer *4852-H
60 mL *Cobalt Indicator Reagent *4853-H
30 mL *Stabilizer Solution *4854-G
2 Pipet, 1.0 mL, plastic 0354
1 Pipet, 0.5 mL, plastic 0353
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
Cobalt rarely occurs in natural water. It is used in the manufacture of alloys
to increase corrosion resistance and strength. It is found in wastewaters as a
corrosion by-product.
APPLICATION: Industrial wastewater.
RANGE: 0.00–2.00 ppm Cobalt
MDL: 0.04 ppm
METHOD: PAN (1-[2-Pyridylazo]-2-Naphthol) forms a greenish
complex with Cobalt (Co
SAMPLE HANDLING
& PRESERVATION:
Store samples in acid-washed plastic bottles. Adjust
pH to less than 2 with nitric acid. Adjust sample pH to 5
before testing.
INTERFERENCES: Iron (+2) and high concentrations of heavy metals.
+2
) at a pH of 5.
Test Procedures
COD3 Plus Colorimeter 3.11 COBALT
www.GlobalTestSupply.com
Page 98
PROCEDURE
1. Press and hold until colorimeter turns on.
2. Press
3. Select ALL TESTS (or another sequence containing 008 Cobalt) from
4. Scroll to and select 008 Cobalt from menu.
5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample.
6. Insert the tube into chamber, close lid and select SCAN BLANK.
7. Remove the tube from colorimeter.
8. Use the 1.0 mL pipet (0354) to add 1 mL of *Cobalt Buffer (4852). Cap and
9. Use the other 1.0 mL pipet (0354) to add 1 mL of *Cobalt Indicator Reagent
10. Wait 3 minutes.
11. Use the 0.5 mL pipet (0353) to add 0.5 mL *Stabilizer Solution (4854). Cap
12. Wait 5 minutes. DO NOT MIX.
13. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result
14. Press to turn the colorimeter off or press
ENTER
to select TESTING MENU.
TESTNG MENU.
mix.
(4853). Cap and mix.
and invert 15 times to thoroughly mix.
in ppm cobalt.
menu or make another menu selection.
EXIT
to exit to a previous
NOTE: For best possible results, a reagent blank should be determined to
account for any contribution to the test result by the reagent system. To determine
Test Procedures
the reagent blank, follow the above test procedure to scan a distilled or deionized
water blank. Then follow the above procedure to perform the test on a distilled or
deionized water sample. This test result is the reagent blank. Subtract the reagent
blank from all subsequent test results of unknown samples. It is necessary to
determine the reagent blank only when a new lot number of reagents is obtained.
COBALT COD3 Plus Colorimeter 3.11
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Page 99
COLOR
PLATINUM COBALT METHOD
NO REAGENTS REQUIRED
Color in water may be attributed to humus, peat, plankton, vegetation, and
natural metallic ions, such as iron and manganese, or industrial waste. Color is
removed to make water suitable for domestic and industrial use. Color may have
to be removed from industrial waste before it is discharged to a waterway.
APPLICATION: Potable water and water with color due to natural
materials.
RANGE: 0–1000 color units
MDL: 20 Cu
METHOD: Color is determined by a meter that has been
calibrated with colored standards of known platinum
cobalt concentration. True color, the color of water in
which the turbidity has been removed, is measured.
SAMPLE HANDLING &
PRESERVATION:
INTERFERENCES: Turbidity will interfere. Filter before testing.
Collect all samples in clean glassware. Determine
color as soon as possible to avoid biological or
chemical changes that could occur in the sample
during storage.
Test Procedures
COD3 Plus Colorimeter 3.11 COLOR
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Page 100
PROCEDURE
1. Press and hold until colorimeter turns on.
2. Press
3. Select ALL TESTS (or another sequence containing 009 Color) from
4. Scroll to and select 009 Color from menu.
5. Rinse a tube (0290) with color-free water (distilled or deionized water). Fill to
6. Insert the tube into chamber, close lid and select SCAN BLANK.
7. Remove tube from colorimeter. Empty tube.
8. Rinse tube with sample water. Fill to 10 mL line with water sample.
9. Insert tube with sample water, close lid and select SCAN SAMPLE. Record
10. Press to turn the colorimeter off or press
ENTER
to select TESTING MENU.
TESTING MENU.
10 mL line with color-free water.
result in color units.
menu or make another menu selection.
EXIT
to exit to a previous
Test Procedures
COLOR COD3 Plus Colorimeter 3.11
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