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1200-UDV COLORIMETER
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CHLORINE
MODEL 1200-UDV-CL · CODE 3670-UDV
QUANTITY CONTENTS CODE
5 Free Chlorine, pack of 5, Unit Dose 4311-C
5 Total Chlorine, pack of 5, Unit Dose 4312-C
2 Unit Dose Vials, empty 29653
1 Water Sample Bottle 28021
1 Cap 28560
1 Syringe, plastic, 3 mL 1184
1 1200-UDV Colorimeter for Chlorine DPD 26742
*WARNING: Reagents marked with a * are considered hazardous substances. Material
Safety Data Sheets (MSDS) are supplied for these reagents. For your safety, read label
and accompanying MSDS before using.
To order individual reagents or test kit components, use the specified code number.
INTRODUCTION
Chlorine is added to water to kill bacteria and other disease-producing organisms,
control algae, and remove undesirable odors and colors. Chlorine added to water quickly
forms hypochlorous acid, HClO, also known as Free Available Chlorine, the active
ingredient responsible for chlorine’s sanitizing capabilities. Free Available Chlorine
combines with impurities in the water to form chloramines and other organic nitrogen
compounds. In combined form, its sanitizing capability diminishes, and higher levels of
chlorine are necessary to achieve effective sanitation. Therefore, it is essential to
chlorinate to the point of establishing a Free Available Chlorine Residual, and then to
maintain that residual at a recommended level.
NOTES: If either chlorine reading displays , repeat procedure on diluted sample,
and multiply the result by the appropriate dilution factor. See 1200 Colorimeter
Instruction Manual for procedure.
Levels of chlorine above 10 mg/L will cause a bleaching effect on the DPD indicator, and
may give a false indication that no chlorine is present. If it is possible that the chlorine
concentration is greater than 10 mg/L (e.g. after shock treatment), perform test on a
diluted sample and multiply the result by appropriate dilution factor.
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CHLORINE TEST PROCEDURE - DPD METHOD
Fill the Water
Sample Bottle
(28021) with
sample water. This
will be used to
dispense sample
water for the tests.
Push the READ
button to turn the
meter on. Press the
ZERO button
and hold it for 2
seconds until bla is
displayed. Release
the button to take a
zero reading (0
ppm). Remove the
vial.
Insert the
vial into the
chamber.
Close the lid.
Push the
READ button.
Record results as
ppm Free
Available
Chlorine.
Rinse and fill
an empty vial
(28560) with
sample water.
Wipe dry.
7. 8.
5.
3.
1. 2.
4.
6.
Use the syringe
(1184) to add 3 mL
of sample water to a
Free Chlorine Unit
Dose Vial (4311).
Invert 3 times to mix.
Wipe vial dry. Make
reading within 30
seconds.
Insert the vial
into the
chamber. Close
the lid. This
vial is the
blank or zero.
I200 COLORIMETER
• • • • • • • • • • • • • • • • • •
READ
bla
ZERO
ZERO
I200
COLORIIMETER
• • • • • • • • • • • • • • • • • •
READ
2.55
3
L
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L
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Read the 1200 Colorimeter Manual before proceeding. Carefully
wipe tubes dry before inserting into the colorimeter chamber.
FREE AVAILABLE CHLORINE
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TOTAL RESIDUAL CHLORINE
Fill the Water Sample
Bottle (28021) with
sample water. This will
be used to dispense
sample water for the
tests.
Push the READ
button to turn the
meter on. Press the
ZERO button
and hold it for 2
seconds until bla is
displayed. Release
the button to take a
zero reading
(0 ppm). Remove
the vial.
Insert the vial
into the
chamber.
Close the lid.
Push the
READ button.
Record results as
ppm Total
Residual Chlorine.
Rinse and fill
an empty vial
(28560) with
3 mL sample
water. Wipe
dry.
7. 8.
5.
3.
1. 2.
4.
6.
Use the syringe
(1184) to add 3 mL
of sample water to a
Total Chlorine Unit
Dose Vial (4312).
Invert 3 times to mix.
Wipe vial dry. Wait 2
minutes for full color
development*.
Insert the vial
into the
chamber.
Close the lid.
This vial is the
blank or zero.
I200 COLORIMETER
• • • • • • • • • • • • • • • • • •
READ
bla
ZERO
ZERO
I200
COLORIIMETER
• • • • • • • • • • • • • • • • • •
READ
2.55
3
*For wastewater samples, Standard Methods for the
Examination of Water and Wastewater recommends
waiting 2 minutes for full color development.
L
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L
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COMBINED CHLORINE
Subtract the Free Available Chlorine reading from the Total Residual Chlorine reading
to determine ppm Combined Chlorine (Monochloramine, Dichloramine, and Nitrogen
Trichloride) present in the water sample.
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DPD CHLORINE TEST METHOD SPECIFICATIONS
APPLICATION
Drinking water supplies and distribution systems, swimming pool and spas, sewage and
chlorinated waste waters, process waters and sanitizing solutions.
RANGE
0 to 4.0 mg/L Chlorine (may be extended by dilution)
METHOD
In the absence of Iodide, Free Available Chlorine reacts instantly with the buffered
diethyl-p-phenylenediamine indicator (DPD) to produce a red color in proportion to the
amount of chlorine present. The addition of potassium iodide produces a rapid color
response from the combined forms of chlorine (chloramines).
HANDLING & PRESERVATION
Chlorine in aqueous solutions, particularly weak solutions, is not stable. Exposure to
sunlight or agitation will accelerate the reduction of chlorine. Fill sample containers to
the top and cap tightly. Analyze samples as soon as possible after collection.
INTERFERENCES
The only interfering substance likely to be encountered is oxidized manganese. The
extent of this interference can be determined by treating a sample with sodium arsenite
to destroy the chlorine present, so that the amount of interference can be measured.
CALIBRATION
The single test colorimeter is precalibrated. In order to comply with NPDWR or NPDES
reporting regulations, the calibration should be checked periodically by using a set of
reference standards including a 0 mg/L blank and 0.3, 1.0, and 3.5 mg/L chlorine. To
prepare these standards, a LaMotte 1000 mg/L standard chlorine equivalent solution
(Code 3858) is available. Consult with your local regulatory agency to determine
standardization frequency.
EPA APPROVED COMPLIANCE MONITORING OF WASTEWATER
Order Total Residual Chlorine, LaMotte Method 7916 (Code 7916-EPA). Follow all
sections except substitute the “Total Residual Chlorine ” section of the 3670-UDV
instructions for “11.0 Procedure.” This will provide a method for the Model
1200-UDV-CL colorimeter which follows an EPA approved method for compliance
monitoring of wastewater.
LaMOTTE COMPANY
Helping People Solve Analytical Challenges
PO Box 329 • Chestertown • Maryland • 21620 • USA
800-344-3100 • 410-778-3100 • Fax 410-778-6394
Visit us on the web at www.lamotte.com
SM
3670-UDV · 11/01
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