LaMotte 1200-NA User Manual
1200 COLORIMETER
NITRATE NITROGEN
MODEL 1200-NA · CODE 3677-01
QUANTITY CONTENTS CODE
2 x 120 mL *Mixed Acid Reagent *V-6278-J
10 g *Nitrate Reducing Reagent *V-6279-D
1 Spoon, 0.1 g, plastic 0699
1 Graduated Cylinder 0416
1 Colorimeter Tubes, w/caps 0290-6
1 Water Sample Collecting Bottle 0688
1
*WARNING: Reagents marked with a * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD
or www.lamotte.com. To obtain a printed copy, contact LaMotte by email, phone or fax.
To order individual reagents or test components, use the specified code number.
NITRATE - NITROGEN INTRODUCTION
Nitrogen is essential for plant growth, but the presence of excessive amounts in water
supplies presents a major pollution problem. Nitrogen compounds may enter water as
nitrates or be converted to nitrates from agricultural fertilizers, sewage, industrial and
packing house wastes, drainage from livestock feeding areas, farm manures and legumes.
Nitrates in large amounts can cause “blue babies” (methemoglobinemia) in infants less
than six months of age. Nitrate concentration is an important factor to be considered in
livestock products, where, in addition to causing methemoglobinemia, it is responsible
for many other problems. Nitrates in conjunction with phosphate stimulate the growth
of algae with all of the related difficulties associated with excessive algae growth.
U.S. Public Health Service Drinking Water Standards state that 10 ppm nitrate nitrogen
should not be exceeded. To the sanitary and industrial engineer, concentrations of less
than 1 ppm are acceptable.
1200 Colorimeter for Nitrate Nitrogen
26734
1
NITRATE-NITROGEN TEST PROCEDURE -
Insert the tube
into the
chamber, being
sure to align the
index line with
the arrow on
the meter.
Close the lid.
This tube is the
blank or zero.
8.
5.
3. 4.
Fill the Water
Sample Collecting
Bottle (0688) with
sample water. This
will be used to
dispense sample
water for the tests.
Rinse and fill a
colorimeter tube
(0290) to the 10
mL line with
sample water.
Cap and wipe
dry.
1. 2.
I200 COLORIMETER
• • • • • • • • • • • • • • • • • •
READ
bla
ZERO
7.
Remove tube from
colorimeter and
pour off 5 mL into
graduated cylinder
or similar. Discard
the remaining
sample.
6.
Pour the 5 mL
sample from a
graduated
cylinder or
similar into the
colorimeter
tube.
Push the READ
button to turn the
meter on. Press the
ZERO button
and hold it for 2
seconds until bla is
displayed. Release
the button to take a
zero reading
(0.00 ppm).
Use the graduated
cylinder or similar to
measure 5 mL of
*Mixed Acid
Reagent (V-6278)
and add to tube.
5 mL
5
m
L
2
M
I
N
U
T
E
S
5 mL
Cap and mix. Wait approximately 2
minutes before proceeding to Step 9.
L
Mott
CADMIUM REDUCTION METHOD
Read the 1200 Colorimeter Manual before proceeding. Carefully
wipe tubes dry before inserting into the colorimeter chamber.
NITRATE - NITROGEN
2
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