Labomed Lx POL User Manual

To ensure proper use of this instrument as well as to avoid injury while operating instrument, understanding this manual completely before use is highly recommended.

User Manual

Lx POL

Research Microscopy

Lx POL

CONTENTS

2 SAFETY INFORMATION 2-5 a. General Instructions b. Symbol Used c. Maintenance and Care d. Health Risks e. Electric Data

1 INT RODUC TION 1

6 STANDARD COMPONENTS 9

5 UNPACKING YOUR MICROSCOPE 8

4 Lx POL TRINOCULAR 7

3 Lx POL BINOCULAR 6

7 OPTIONAL ACCESSORIES & INSTALLATION 10-11

8 DETAILED INTRODUCTION OF ASSEMBLIES 12-14 a. Flip Top Abbe Condenser b. Round Stage c. Centerable revolving nose piece d. Objectives e. Eye pieces f. Bertrand Lens

9 INITIAL SETUP 15-16 a. Observation Head b. Eye Pieces c. Mounting the Day Light (Blue) Filter d. Connecting Power Cords

CONTENTS

10. CENTRATION 17-19 a. Preparation for centration b. Kohler illumination centration c. Flip Top Abbe Condenser centration d. Objective centration e. Extinction adjustment f. Conoscopical adjustment

11. SUMMARY OF POLARISED OBSERVATION PROCEDURE 20

12. SUMMARY OF POLARISED OBSERVATION PROCEDURE 21-24 a. Placing the specimen on stage b. Adjusting the focus c. Adjusting the Interpupillary Distance d. Adjusting the Diopter e. Adjusting the Condenser position and Aperture Iris Diaphragm f. Switching the objectives g. Using the 100x Immersion Objective

13. TROUBLESHOOTING GUIDE 25-26

14. SPECIFICATIONS 27

INTRODUCTION

The Lx POL is a research polarizing microscope reflecting a modern design as well as the latest in optical and mechanical advancements. Designed for professionals, this microscope offers many features and functions for a diverse set of applications. It is a truly professional polarizing Microscope that meets, and indeed exceeds the quality of some of the competing Microscopes. Here are a few points highlighting the benefits of the Lx POL:

- Extra clarity and contrast is provided through a 360 degree rotatable viewing body inclined at 30 degree with IPD adjustments.

- The pressure die cast stand consists of ball bearing, frictionless sideways focusing to avoid any loss in motion.

- The sturdy new stylish design provides a high degree of comfort as well as stability.

- The high powered objectives are spring loaded to prevent accidental damage to specimen slides.

- The highly precision Parfocalized & Parcentered reverse angle quadruple nosepiece has the provision of centering for all objectives .

- The round ball bearing Rotatable Stage has smooth 360 degree travel and has 1 degree graduations on the scale which provides accurate location of the specimen.

- High power illumination is delivered through our well crafted Universal Power Supply and operates on 100V- 240V AC constant input.

- Halogen bulb (6V-20W) has an average life span of up to 2,000 hours.

- The Lx POL is equipped with a Flip Top Abbe Condenser N.A. 1.25 and a swing in and swing out provision for the Top Lens to attain for brighter illumination levels. An iris diaphragm is also provided for better resolution and contrast control.

- High quality Polarizer & Analyzer filters for perfect extinction level.

- Polarizer: This is provided below the condenser and is 360 degree graduated and lockable in any desired position.

- Analyzer : The advances Analyzer module is located below the viewing tube and provides specific cross polarization through the full 90 degree quadrant.

- A center adjustable focusable Bertrand lens is a standard feature in the module which provides conoscopic observation.

- A set of high quality compensators for advanced polarized observation include Gypsum full wave length Quartz Wedge and a Mica 1/4 wave plate.

1. A microscope is a precision instrument with delicate glass components, please handle with care

2. Do not use the microscope where it is subjected to direct sunlight, high temperature, humidity, dust and vibrations.

3. The microscope is ventilated by natural convection. Be sure to leave enough space (10 cm or more) around body when installing the unit.

4. Arm handle is provided for carrying the microscope.

To prevent damage, do not hold the microscope by the stage or observation tube. Be sure to remove the specimen

from the stage clip while transporting unit to avoid damage to the specimen slide.

Symbol Explanation

This surface has a tendency to heat up and should not be touched unless system has completely cooled down.

Before use, carefully read the instruction manual. Improper use could result in injury to the user and/or damage to the equipment.

Main switch is ON.

Warning against risk of electric shock.

Main switch is OFF.

The following symbols are found on the microscope. For optimal use, it is recommended that users understand these symbols and always use the equipment as prescribed.

SAFETY INFORMATION

Lx POL

if the microscope is used in a manner not specified by this manual, the safety of the user may not be warranted. In addition, the equipment may also suffer damage. Always use the equipments as outlined in this instruction manual.

I) General Cleaning

Your Microscope has been engineered for a long and safe operational life with the least amount of maintenance required. In general, routine maintenance is limited to keeping the microscope working parts lubricated and optics clean.

 Clean all glass components by wiping gently with cleaning cloth provided. To remove fingerprints or oil

smudges, wipe with cleaning cloth slightly moistened with a mixture of petroleum (85%) and isopropanol (15%)

Since solvents such as petroleum and isopropanol are highly flammable, they must be handled carefully. Be sure to keep these chemicals away from open flames or potential sources of electrical sparks - for example, electrical equipment that is being switched “ON” or “OFF”. Also remember to always use these chemicals only in a well-ventilated room.

 Do not attempt to use organic solvents to clean the microscope components other than the glass

components. To clean non-glass components, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

General Instructions

Safety Symbols

Maintenance and Care

3. Do not disassemble any part of the microscope as this could result in malfunction or mitigated performance.

4. When not using the microscope, ensure that the frame is fully cooled before storing the unit in a dry locker or covering with a dust cover (provided).

5. To clean the Flip Top Condenser, fully loosen the securing thumb screw (1) and remove the condenser then, wipe the front lens of the condenser with optical cleaning solution (mixture suggested above) and lens tissue.

The Flip Top Abbe can be re-attached in its seat, by tightening securing thumb screw, and raising condenser

bracket to desired position. (As shown in picture)

6. Be sure to observe you local rules / regulations for product disposal.

7. Always cover the Microscope with the provided dust cover when not in use

II) Optical Cleaning

1. The objectives have been adjusted for a tight fit to prevent any damage during transportation. To remove an objective, rotate it counterclockwise while gripping it with a rubber sheet, etc. to avoid any slippage.

2. To clean the lens surfaces, remove dust using a soft brush or compressed air (cans available at your local electronic store). For removing finger marks or grease, soft cleaning cloth or lens tissue lightly moistened wit cleaning solution (85% petroleum ether and 15% isopropanol) should be used. For cleaning the objective optics, use Meathanol. Observe sufficient caution in handing Methanol. Place the Objectives and / or eyepieces on a dust free surface (e.g. aluminium foil). All other optical components to be cleaned should be as accessible as possible.

3. Blow all loose dust particles away with compressed air of mini dust blower.

4. Remove all water-soluble dirt with distilled water. If this is unsuccessful repeat using a solution of diluted hand soap liquid. Remove any remaining residue with a dry cotton slab.

5. To remove oil, use a solution of diluted hand-soap liquid initially. If this does not produce a satisfactory result, repeat the cleaning using a solvent (Optical Cleaning Solution 85% petroleum ether and 15% isopropanol).

6. Grease must always be removed using a solvent.

7. Cleaning is achieved by using a spiral motion from the center to the rim. Never wipe using zig-zag movements as this will only spread the dirt. With larger optical surfaces (e.g. tube lenses) the spiral motion starts initially at the rim before moving to the middle and is then followed by a center to rim cleaning motion. Normally several spiral wipes are recommended.

We recommend pure, volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above.

Wipe using a spiral movement Donot use a zig-zag motion

III) Cleaning of Painted Surfaces

Avoid the use of any organic solvent (e.g. thinner, xylene, ether, alcohol etc.) for cleaning of painted surfaces of the instrument. Painted surfaces can be cleaned with a very lightly moistened micro fiber cloth. Loose dust and other dirt particles can be removed using a soft bristle brush used exclusively for this purpose.

This microscope has an ergonomic design that ensures minimum exertion of the user. However, some of the

risks that the user should keep in mind are:

i) Risk of Infection:

After the microscope has been used for observation of a specimen containing bacteria, clean all parts coming in

contact with the specimen to prevent infection.

1. Be sure to remove the specimen before moving this product.

2. In case the specimen is damaged by erroneous operation, it is important to clean all surfaces that may come in contact with the specimen.

ii) Electrical Hazards:

To avoid potential electrical hazards when replacing halogen bulb turn the microscope’s main switch to the OFF

position and disconnect power cord from wall outlet in advance. Whenever you replace your microscope bulb, allow lamp socket and bulb to cool before touching.

I) General Instructions:

1. Install microscope on a sturdy, level table or bench and aovid any restriction of air vents in the base of the unit. Do not place microscope on a flexible surface as this could result in blocking the air vents and cause overheating.

2. Always use the power cord provided by LABOMED. If the proper power cord is not used, product safety performance cannot be warranted.

3. When installing the Microscope, route the power cord away from the microscope frame, Should the power cord come in contact with the Microscope base, the power cord could melt due to overexposure heat.

4. Always ensure that the grounding terminal of the Microscope and that of the wall outlet are properly connected. If the unit is not grounded. LABOMED can not warrant electrical safety.

5. Never allow metallic objects to penetrate the air vents of the Microscope frame as this could result in user injury and damage to the Microscope.

6. After operation of Microscope, be sure to disconnect power cord from connector socket of the Microscope or from the wall power outlet.

Health Risks

Electrical Data

1. Before attaching the lamp bulb, remove the parts that may drop such as the filter and specimen from the Microscope frame, and place the Microscope on its back so that the bottom plate is exposed.

2. Pull the lock know (1) on the bottom to open lamp housing door

(fig. 1)

3. Hold the halogen bulb (2) without taking it out of the polyethylene bag so as not to taint the bulb with fingerprints and push the bulb into the pin holes on the socket (3). After attaching, remove the polyethylene bag.

4. With the lock knob pulled out, close the lamp housing door, then push the lock knob back to lock the cover. Always use the designated bulb. Using a bulb other than those specified by LABOMED may lead to a fire hazard. Fingerprints or stains on the lamp bulb reduce its life. If contamination occurs, wipe bulb surface with a cloth slightly moistened with alchohol.

Applicable Bulb: 6V20W Halogen Bulb P/N CX-013

Fig. 1

Caution: For Fuse replacement Set the main switch to “O” (OFF), disconnect the power cord from the wall outlet.

Before replacing the fuse, remove the parts that may drop such as the filter and specimen from the microscope frame. Turn around the microscope to its back so that the AC inlet is visible.

1. Use a flat head screw driver to open the fuse holder (1).

2. The fuse tray will come out with (2) live fuse and (3) spare fuse. Do

not pull out the fuse tray with force as it is locked and will not be out completely.

3. Replace the primary fuse (2) with the spare fuse.

4. Engage the fuse tray back in.

Always use the designated Fuse. Using a fuse other than those specified by LABOMED may lead to a fire hazard.

Fig. 2

II) Bulb Replacement

III) Installing or Replacing the Fuse

Lx POL Binocular

Lx POL

Binocular viewing tube 30° inclined

Eyepieces

Intermediate Analyzer Kit

Bertrand Lens centering screw

Centerable Revolving Nosepiece

Polarizing Objectives

Specimen Holder

Vernier Scale

Flip Top Abbe Condenser

Polarizer

Koehler mount

Coarse and fine focus knob

Polarizer locking screw

Intensity regulator

Bertrand lens focusing knob

Bertrand lens on/off

Allen keys for objective centering

Parking for additional wave plates (2)

Analyzer on/off

Stage locking screw

Circular stage with vernier

Port of wave plate

Day light blue filter

Condenser Centering Screw

Lx POL

Lx POL Trinocular

Eyepieces

Centerable Revolving Nosepiece

Polarizing Objectives

Specimen Holder

Flip Top Abbe Condenser

Koehler mount

Intensity regulator

Coarse and fine focus knob

Stage locking screw

Circular stage with vernier

Port of wave plate

Analyzer on/off

Parking for additional wave plates (2)

Allen keys for objective centering

Trinocular viewing tube, 30° inclined

Bertrand lens focusing knob

Bertrand lens on/off

Intermediate Analyzer Kit

Bertrand Lens centering screw

Vernier Scale

Polarizer

Day light blue filter

Polarizer locking screw

Lx POL

Condenser Centering Screw

Lx POL

UNPACKING YOUR MICROSCOPE

Microscope Arm

Observation head

Eyepieces

Power Cord

 After removing your microscope from its packaging, make sure that all of the following contents are present.

“Please note that the contents of your microscope may vary as the optional configuration, contrasting method or

viewing body opted for may not be of the standard configuration highlighted here”

STANDARD COMPONENTS

Lx POL

Daylight (blue) filter

Power Cord

Allen Wrench 3mm

Polarizing attachment

Paired Eyepieces

6V 20W Halogen bulb

Binocular viewing tube 30° inclined

Eyepieces

Intermediate Analyzer Kit

Bertrand Lens centering screw

Centerable Revolving Nosepiece

Polarizing Objectives

Specimen Holder

Vernier Scale

Flip Top Abbe Condenser

Polarizer

Koehler mount

Coarse and fine focus knob

Polarizer locking screw

Intensity regulator

Bertrand lens focusing knob

Bertrand lens on/off

Allen keys for objective centering

Parking for additional wave plates (2)

Analyzer on/off

Stage locking screw

Circular stage with vernier

Port of wave plate

Day light blue filter

Condenser Centering Screw

Lx POL

System Diagram of Optional Accessories

OPTIONAL ACCESSORIES

Binocular head

Flip Top Abbe Condenser

Blue filter

RP 2.5x RP 10x

RP 20x RP 40x (SL) RP 100x (SL, Oil)

Trinocular head WF 10x WF 16x WF 20x

Halogen Bulb

iVu 5100

iVu 7000

RP 4x

Polarizing kit

Analyzer Module

Installation and Operation of Optional Accessories

1. Mount the Video adapter 1/2” (part # 3143300-912) Fig. 4 on Trinocular observation head (Fig. 5).

2. Mount iVu Camera Module System (Fig. 3) on video adapter.

iVu Camera Module System

10X eyepieces are provided. To replace:

1. Pull out the 10x eyepieces out from the observation heads ocular tube.

2. Insert desired eyepieces in empty ocular tube.

Optional Eyepieces

Fig. 3

Lx POL

Fig. 6

Fig. 5

Fig. 4

Flip Top Abbe Condenser

Lx POL Flip Top Abbe condenser is has three very important basic requirements i.e.

1. Stain free optical system.

2. The feature to swing in & out the top lens of the Abbe Condenser in the light path in order to achieve almost parallel illumination wave fronts for low magnification and birefringence observation. Also equipped with iris diaphragm (as shown in fig. 7 respectively).

3. Rotate knurled ring to open and close IRIS Diaphragm for respective Objective magnification in order to match numerical aperture.

Round Stage

Lx POL round stage has both the key feature of rotatability & centerability (Fig. 9). Lx POL round stage is equipped with the key features of rotatability and centerability (Fig 9). It provides a 360 degree circular rotation which allows the user to study the orientation by coinciding the objective centration with the microscope's optical axis. This makes the center of rotation coincide with the center of field of view. Rotatibility allows the user to observe the specimen in the diagnol position ( the brightest position of anisotropy).

It is equipped with a vernier scale to measure the accuracy of 0.1 degree and locking provision to stop the stage at desired location. Lx POL round mechanical stage is based on hard steel ball bearing which provides even and smooth jerk free motion across 360 degree.

DETAILED INTRODUCTION OF ASSEMBLIES

Lx POL

Fig. 7

(Top lens out from Light Path)

Top Lens

Iris shifter

(Top lens in Light Path)

(Polarizer out of Light Path)

This high quality primary polarizer (1) is 360 degree rotatable with fiducial marking (2) for quick identification of Polarized / Cross Polarized positions.

It is ideally located below condenser and with a lockable mechanism (3) to stop polarizer filter at desired position for comfortable observation experience.

Lx POL Polarizer is also equipped with a mechanism of swing in / out the polarizer from the light path

Polarizer

Objectives

Lx POL objectives are free from stress & both type of 'strain' i.e. glass characteristics during various stages of the assembly like cementing or mounted in close proximity with tightly fitted frames. Lx POL objectives are marked P on objectives barrel only after passing the strict testing. Lx POL comes with standard 4x, 10x, 40x objectives that solve the purpose of viewing specimen in conoscopic & orthoscopes modules. These objectives are anti-refraction coated. Some other objectives like

2.5x, 20x & 100x are also available.

Fig. 11

LABOMED

USA

RP POL PLAN ACHRO

40X / 0.65 / 0.17

9124040

LABOMED

USA

RP POL PLAN ACHRO 10X / 0.25 / 0.17

9124010

Centerable revolving nose piece

Lx POL comes with a centerable revolving nose piece to compensate optical axis of each objective as they vary from one assembly to another. Lx POL Nose Piece is fully equipped with centering mechanism for each objective to make sure that each object is centered to the stage and microscopic optical axis so that the specimen remains in the center when the stage is rotated. Movement of the turret is based on hard steel ball bearing which provides smooth and jerk free motion around 360 degree. This nose piece is highly precision parfocalized and par centered.

Fig. # 10

1. Objective Centering Allen Screws.

2. Nose Piece Cover

3. Port for Lamda Plate

Centerable revolving turret is also equipped with a Nose Piece cover which provides a click stop lockable port for Gypsum Lambda wave plates fig. 10-a. This feature provides comfort while using different Lambda Wave Plates. It is ideally located in between specimen & the Analyzer. It allows to introduce compensator & retardation plates between the cross polarizer which can enhance optical path differences in the specimen details.

Lx POL

Fig. 10

Fig. 10-a

Quartz Wedge

1-4 Order

Bertrand Lens

Lx POL comes with a Bertrand Lens that projects an interference pattern formed at the objective rear focal plane into focus at the microscope image plane. It is capable of examining the objective rear focal plane, to ensure exact adjustment of the illuminating aperture diaphragm and to view interference images. These images form in the objective rear focal plane when an optically anisotropic specimen is viewed between cross polarizer using a high numerical aperture objective / condenser combination. It is ideally located between analyzer and eye pieces for an easy in and out movement from the light path. Lx POL Bertrand lens is also centerable with respect to the optical axis of the microscope and has focusable mechanism for a comfortable viewing experience.

Fig. # 13 & 14

1. Bertrand Lens Focusing Knob

2. Bertrand Lens Operating Lever

3. Analyzer Operating Lever

4. Gypsum wave Plate

5. Allen wrench for Objective centering.

6. Allen screw for Bertrand Lens Centering.

Fig. 13 : Bertand Lens lever (2) in out “O” position i.e. away from light path. Analyzer Lever (3) in out “O” position i.e. away from light path.

Fig. 14 : Bertand Lens lever (2) in “BL” position i.e. in light path. Analyzer Lever (3) in “A” position i.e. in light path.

Fig. 13

Fig. 14

1 2

Eye Pieces

Lx POL comes with a pair of Eye Pieces. One of the eyepieces has a crosshair reticule to mark the center of the field of view and the second for normal viewing. Orientation of the Eye Piece with respect to the Polarizer and Analyzer is ensured by the eye tube lower that slides into the observation Bino Body tube. These eye pieces also have a focusing mechanism for diopter correction & foldable eye guards to prevent ambient light from entering into your line of vision.

Fig. 12

INITIAL SETUP

Observation Head

Eyepieces

Install the observation head using the following procedure:

1. Using the 3mm allen wrench (provided), loosen the Head Locking Screw (1) and remove the dust cover cap provided in the dovetail cavity.

2. Mount the Analyzer attachment by engaging the dovetail provided on the attachment into the dovetail cavity on the microscope arm and tighten the Head Locking Screw (1) See figure 15.

3. Remove the Dust Cover Cap from Observation Head Dovetail and mount the Observation Head by engaging the dovetail provided at the bottom of the head into the dovetail cavity on the analyzer attachment.

4. Tighten the Head Locking Screw (2) after positioning the Observation Head as desired. See figure15.

Fig.15

Fig.16

Insert the eyepieces into the ocular tube of Observation Head using the following procedure:

1. Remove the protective caps from the observation tube

(See figure16).

2. Insert eyepieces into the ocular sleeve for use (See figure17).

Lx POL

Fig.17

Fix Stage Clips on round rotatable stage for holding the pol specimen.

Attach the power cord and plug it into a grounded electrical outlet.

1. Flip the main switch to ”I” (ON) as shown in Figure 20.

2. Rotating the light intensity adjustment knob in the direction of the

arrow increases brightness and rotating knob in the opposite direction decreases brightness. The intensity bar next to the knob indicates the direction of intensity level.

Note: Never use an adapter between the power cord and the power source; it will render the microscopes grounding feature ineffective.t

Stage Clips

Connecting Power Cord

Fig. 19

Fig. 20

On/Off SwitchElectrical outlet

Fig. 18

This filter modifies the color of observation light into a natural (daylight) color.

Place Blue filter on Koehler Mount.

Mounting the Daylight (Blue) Filter

Preparation for Centration

Step: 1

Disengage the Analyzer and Bertrand Lens by switching the operating lever to “O” position as shown in Fig. 13. Fully open the aperture diaphragm of the condenser by rotating its ring to the extreme left.

Lower the microscope stage. Place a POL specimen on the stage. Swing in the 10x objective into working position. Raise the Microscope stage using the coarse adjustment knob until you reach its positive stop. Use the fine adjustment knob to bring the POL specimen section into focus by lowering down the sage.

Koehler Illumination Centration

Step: 2

Swing in the 10x objective into working position. Flip in top lens of Flip Top condenser in light path. Close the Koehler field diaphragm so that its closed iris leaves image is present within the field of view. Use the condenser focusing knob to bring the image into sharp focus. Operate the condenser centering screws simultaneously to Center the image of the field diaphragm Ref. Fig. 22 (a), (b), (c). After centration open the field diaphragm until the Image (iris leaves) disappears just beyond the field of view.

Flip Top Abbe Condenser Centration

Step: 3

Look through 10x Objective & Eye Pieces cross hair scale. Close the condenser diaphragm until approximately 20-25% of the iris leaves fill the field of view. Adjust the centering of the diaphragm by maintaining condenser centering screws simultaneously with reference to Eye Piece cross hair scale Ref. Fig. 23 (a).

Note: When changing the objectives, adjust the condenser field diaphragm with respect to each objective.

CENTRATION

Fig. 23

Lx POL

Fig. 22

Fig. 23 (a)

(a) (b) (c)

Lx POL

22 22 2222

Objective Centration

Swing in 10x objective in the light path. Adjust the condenser aperture diaphragm. Retrieve the two centering keys (Ref Fig 13 Part 5) and insert them in the centering holes (Ref Fig 10 Part 1) above the objective you want to center. Focus the POL sample.

I) Bring some prominent point of the specimen to the center of the Eye

Piece crossline (Fig 24 (a))

ii) Loosen the stage locking thumb screw which will allow the Round

Stage to rotate 360 degree horizontally. Rotate the round stage 360 degree until the prominent point of the specimen is furthest away from the center of the Eye Piece crossline, it may even be outside the field of view. (Fig 24 (b))

iii) Adjust the image with the centering screws over the objective until the

prominent point of the specimen is midway between the center of the Eye Piece crossline. (Fig 24 (c))

iv) Adjust the specimen so that the prominent point is at the center of the

Eye Piece crossline. (Fig 24 (d)) Check that it stays at the center of the Eye Piece crossline when the stage is rotated 360 degree. Repeat the centering process if necessary.

Note: Each objective must be centered separately.

(a)

Position 1 Position 1 Position 1

Position 2Position 2

(b) c) (d)

24 24 24 24

Lx POL

Extinction Adjustment

1. Remove all compensator; specimen & test plates out of light path.

2. Swing in 10x Objective in the light path.

3. Swing out Top lens of Flip Top Abbe Condenser from light Path (Ref. Fig. 08)

4. Bring preset vibration direction Analyzer plate by moving Analyzer lever to ‘A’ position (Ref. Fig 14 Part-3)

5. Bring polarizer to light Path (Ref. Fig 25) loosen polarizer lock screw for free 360 degree rotation of polarizer.

6. Rotate polarizer until you achieve complete extinction. Lock Polarizer by tightening Polarizer locking screw.

Conoscopical Observation

1. Bring an objective of your choice between 20x to 100x in the light path. Bring the Pol specimen into focus.

2. Keep the abbe condenser in its lowest position.

3. Swing in Top lens of Flip Top Abbe Condenser in the light path.

4. Open the aperture diaphragm

5. Swing in lever of Bertrand lens to ‘BL’ Ref. Fig 14 Part (2) and focus image by rotating Bertrand Lens focusing knob Ref. Fig 14 Part (1)

Note: If the conoscopic image is dark, then move the condenser upward to find the suitable position where the image is brightest.

Conscopic image may not be at the centre of Eye piece cross line intersection. However it will have no measurable effect due to un i v e r s a l infinity optical design.

Conoscopic Image

Cross Lines

Polarizer away from Light Path

Fig. 25

Fig. 25-a

Fig. 26

Flip the main switch to “ON”

Disengage Analyzer and Bertrand Lens lever to “O” position.

Adjust Koehler aperture iris diaphragm

Bring the specimen in focus & adjust Flip Top Condenser in & out position. Adjust objective cent ration and stage cent ration with respect to Eye Piece cross hair scale.

Adjust the observation tube and Adjust the interpupillary distance. Adjust the dioptric setting.

Engage Polarizer & Analyzer filters in the light path and use Bertrand Lens and Wave plates

Adjust the light intensity

Observe Specimen.

Main switch

Fuse Holder

SUMMARY OF POLARIZED LIGHT OBSERVATION PROCEDURE

eyepieces

Engage the 10x Objective in the light path

Adjust Flip Top Abbe Condenser aperture iris diaphragm.

I 0

Lx POL

Placing specimen on the stage

Fig. 27

DETAILED OBSERVATION PROCEDURE

1. Rotate the coarse adjustment knob (2) in anticlockwise direction to

fully lower the stage.

2. Press the spring loaded clip lever (3) by pushing lever handle (1),

place the specimen by sliding the specimen glass plate(s) on the stage

3. After positioning your specimen slides,(1 max) release the lever

handle.

4. Rotate the stage 360° to achieve best viewing position. The degree

of position can be noted.

“Vernier Scales”

These scales allow for easy identification of the specimen’s position (coordinates), making it easy to return to a particular region of interest after scanning the slide. (Figure 29.)

Cover glass

This is the glass plate placed on the specimen. For optimum optical performance, the cover glass thickness, which is the distance from its surface to the specimen surface, should be 0.17 mm.

Slide glass

This glass plate should ideally have a length of 76 mm, width of 26 mm ±1 mm and thickness between 0.9 and 1.4 mm.

Cover glass

Slide glass

Fig. 28

Fig. 29

Lx POL

Focusing Procedure (Figure 30)

1. Rotate the coarse adjustment knob (1) clockwise so that the

objective (3) is as close as possible to the specimen (We recommend starting with 10X).

2. While observing the specimen through the eyepieces, slowly rotate

the coarse adjustment knob (1) counterclockwise to lower the stage.

3. When coarse focusing of the specimen is obtained (an image is

observed), rotate the fine adjustment knob (2) for fine detail focusing.

Working Distance (WD)

The WD refers to the distance between each objective and the specimen, when acute focus of the specimen is obtained.

Adjusting the Focus

Fig. 30

The inter-pupillary distance adjustment consists of regulating the two eyepieces to align with both eyes’ pupils so that you can observe a single microscopic image through two eyepieces in stereo vision. This greatly helps to reduce fatigue and discomfort during observation.

While looking through the eyepieces, move both eyepieces laterally until the left and right fields of view coincide completely. The position of index dot (•) indicates the inter-pupiliary distance value.

Note your interpupillary distance so that it can be quickly referred to in the future. This happens when multiple users work with the microscope.

Adjusting the Interpupillary Distance (IPD)

Fig. 31

Objectives Eye Piece Flip Top Abbe

10x/20 W.F. (4140010) Condenser Objective N.A. W.D. Cover Resolution Total Field of Depth of Obj. Flip Position Magnification (mm) Glass ( m) mag. view/mm focus( m) N.A. (In / Out)

2.5x (9124002) 0.08 20.0 0.17 5.0 25x 8.0 400 0.08 Out

4x (9124005) 0.10 30.0 0.17 3.36 40x 5.0 200 0.10 Out

10x (9124010) 0.25 4.04 0.17 1.34 100x 2.0 30 0.25 Out

20x (9124010) 0.45 1.10 0.17 0.75 200x 1.0 6 0.45 In

40x (9124010) 0.65 0.45 0.17 0.52 400x 0.50 3 0.65 In

100x (oil) (9124010) 1.25 0.14 0.17 0.27 1000x 0.20 0.70 1.25 In

Adjusting the Diopter

Using the Eye Guards

When Wearing Eyeglasses Use with the eye guards in the normal, folded-down position. This will prevent the eyeglasses from being scratched.

When Not Wearing Eyeglasses Extend the folded eye guards outwards (direction of the arrow) to prevent ambient light from entering into your line of vision.

Fig. 32

Fig. 33

Procedure for adjusting the diopter:

1. Rotate the right eyepiece to match the markings of your IPD (If your

IPD is 64, rotate the eyepiece to 64 mark).

2. While looking through the right eyepiece with your right eye, rotate

the coarse and fine adjustment knobs to bring the specimen into focus.

3. While looking through the left eyepiece with your left eye, rotate only

diopter adjustment ring on the eyepiece until specimen is at its best possible focus.

The condenser is most often used in the highest position. If the observed field of view is not bright enough, brightness may be improved by lowering the condenser slightly

1. Rotate the condenser height adjustment knob (2) to move the

condenser to the highest or desired position.

2. The aperture iris diaphragm ring (1) has an objective magnification

scale. Slide the diaphragm lever left right to achieve the desired illumination level.

3. Swing in the flip top condenser on 40x and 100x magnification. The

flip top condenser should be in swing out position on 4x and 10x objective position.

Adjusting the Condenser Position and Aperture Iris Diaphragm

Fig. 34

Lx POL

Rotate the revolving nosepiece (1) so that the objective to be used is in line above the specimen. Always use the knurled surface to rotate the objective nosepiece.

Switching the Objectives

Fig. 35

The designated immersion oil should be in contact with the cover lens of the 100X immersion objective. If not, the specimen will appear distorted and dull. It is recommended that LABOMED immersion oil is always used.

Immersion Process:

1. Bring the specimen in focus using first the 10x, then 40x objective.

2. Disengage the 40x cycling towards 100x, and place a drop of immersion

oil on the center point of the specimen.

3. Rotate the revolving nosepiece to engage the immersion objective and

rotate the fine adjustment knob to bring the specimen into focus

(Since air bubbles in the oil will affect the image quality, make sure that the oil Is free of bubbles. To remove bubbles, rotate the revolving nosepiece slightly to agitate the oil).

4. The condenser of this microscope manifests the full performance when

oil is placed between the slide glass and the front lens of condenser. If oil is not placed there, the observed image may appear dark.

5. After use, remove oil from the objective front lens by wiping with lens

tissue slightly moistened with an ether (70%) alcohol (30%) mixture.

Caution If immersion oil makes contact with your eyes, rinse eyes out thoroughly with fresh water. If immersion oil makes contact with skin, wash affected areas with soap and water. If prolonged discomfort is experienced, consult your physician immediately.

Using the 100X Immersion Objective

Lx POL

LABOMED USA

RP POL PLAN ACHRO

40X / 0.65 / 0.17

9124040

LABOMED

USA

POL PLAN

ACHRO

10X / 0.25 / 0.17

9124010

Fig. 36

TROUBLESHOOTING GUIDE

Observation

Cause

Remedy

1. Uneven brightness in observation The objective is not engaged in the Engage the objective into position field light path until the nose turret clicks

The condenser is too low Raise up to achieve more light

The objective, eyepiece, condenser Clean them thoroughly as previously and/or window lens are dirty prescribed in “Optical Cleaning”

2. Dust or stains are visible in The eyepiece, condenser, window lens Clean glass parts thoroughly with observation field and/or specimen glass is dirty lens tissue and cleaning solution prescribed in“Optical Cleaning”

3. Glare visible in field of View The condenser is too low Raise condenser light

The condenser iris diaphragm ring is Adjust the aperture according to the closed objective magnification

4. Observation image is hazy The objective is not engaged in the Engage the objective into position or unclear light path until it clicks

The objective, eyepiece, condenser Clean glass parts thoroughly with and/or specimen glass is dirty lens tissue and cleaning cloth

Immersion oil is not used with an Use immersion oil as suggested immersion objective.

Bubbles are present in immersion oil Remove the bubbles by agitation

The specified immersion oil is not used Use the immersion oil supplied by LABOMED

5. Part of image is defocused The objective is not properly engaged Engage the objective into position in the light path until the nose turret clicks

The specimen is not set properly on Set the specimen correctly on the the stage stage and secure using the specimen holder

6. Coarse focus adjustment cannot The condenser is too low Raise the condenser lower the stage low enough

7. Fields of view through both The interpupillary distance is not Adjust IPD to the appropriate setting eyepieces is inconsistent adjusted properly

Dioptric compensation for the two eyes Adjust diopter settings is not set

The left and right eyepieces are of Ensure that both eyepieces are of different magnification are of same magnification. LABOMED does not recommend using third party eyepieces in conjunction with LABOMED eyepieces.

Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take corrective action as needed. If problem persists, please contact LABOMED or your local LABOMED dealer.

Observation

Cause

Remedy

8. Objective hits the specimen when The specimen slide is upside down Set the specimen correctly with the an objective is switched to a higher cover glass facing upwards magnification objective The cover glass is too thick Use a cover glass with thickness of

0.17mm

The stage is raised too high Lower the stage

The slide has slipped from the slide Re-position the slide in the slide holder holder

Slide is of excessive thickness Use slides with thickness between 0.9 and 1.4mm

9. Bulb does not turn On Bulb is not mounted Attach a bulb

Bulb is blown Replace the bulb

The power cord is unplugged / Not firmly Ensure power cord is securilly plugged secured into the box socket + wall outlet

Fuse is blown Check and replace with live fuse

10. Bulb blows easily The specified bulb is not used Replace with the specified bulb

11. Field remain dark even Bulb is on In the Exintetion Position Remove Analyzer from light path by switch Analyzer lever to out “O” position.

Swing out Polarizer from light path. Bertrand Lens in the Light Path (BL) Swing out Bertrand Lens to out “O” position i.e. away from light path.

12. Conoscopic image is not visible Bertrand lens is away from light path. Bring Betrand Lens in light path at “BL” position.

Condenser Top Lens not in the light Swing in Top Lens of condenser in the path. light path.

Using lower magnification objective. Go to specified objective magnification i.e. from 20x to 100x

13. Extinction failure Analyzer & Polarizer out of the light Bring Analyzer & Polarizer in the light path. path.

Analyzer & Polarizer are not in cross Adjust Polarizer by rotating to get positions. complete extinction.

Lx POL

1. Illumination Built-in illumination system Halogen

2. Focusing mechanism Stage height adjustment mechanism Fine adjustment scale: 3.0µm per graduation Fine adjustment stroke: 0.3mm per turn Total stroke: 12.7mm Co-axial coarse and fine focusing on ball drive

3. Revolving nosepiece Quadruple positions fixed (Reverse angle)

4. Observation tube Binocular Trinocular

Field number 20 (Standard) 20 (Standard)

Tube tilting angle 30° 30°

Interpupillary distance 48-75 48-75 adjustment range

5. Round Stage Size Dia 160mm

Rotatability 360 degree

Specimen holder Spring Loaded stage clips

6. Condenser Type Flip Top Abbe condenser (daylight filter detachable)

N. A. 1.25

Aperture iris diaphragm Built-in

7. Dimensions & Weight 405mm (L) x 210mm (W) x 425mm (H); 7 kg net

8. Electrical Halogen 6V-20W upto 2,000 hours

9. Operating environment Indoor use Altitude: Max. 2000 meters Ambient temperature: 5° to 40°C (41° to 104° F) Maximum relative humidity: 80% for temperature up to 31°C (88°F), decreasing linearly through 70% at 34°C (93°F),to 50% relative humidity at 40°C (104°F) Supply voltage fluctuations: Not to exceed ±10% of the normal voltage. Pollution degree: 2 (in accordance with IEC60664) Installation/Overvoltage category: II (in accordance with IEC60664)

SPECIFICATIONS

www.laboamerica.com

Our policy is one of continuous development. Labo America, Inc., reserves the right to change design and specifications without prior notice.

LABOMED and Lx POL are registered trademarks of Labo America, Inc. With a policy of continuous development, Labo America, Inc. reserves the right to change design and specifications without prior notice.

Labo America Inc.

920 Auburn Court Fremont CA 94538

ISO 9001 : 2008 File No. A9020

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Labomed Lx POL User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual