To ensure proper use of this instrument as well as to avoid injury while operating
instrument, understanding this manual completely before use is highly recommended.
User Manual
Lx 400
Research Microscopy
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CONTENTS
9 ASSEMBLY 13-14
12 TROUBLESHOOTING GUIDE 21-22
13 SPECIFICATIONS 32
11 DETAILED OBSERVATION PROCEDURE 16-20
10 SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE 15
8 INITIAL SETUP 12
7 OPTIONAL ACCESSORIES 9-11
6 STANDARD COMPONENTS 8
5 UNPACKING YOUR MICROSCOPE 7
4 Lx 400 TRINOCULAR 6
3 Lx 400 BINOCULAR 5
2 SAFETY INFORMATION 2-4
1 INT RODUC TION 1
The Lx 400 is a research microscope reflecting a modern design as well as the latest in optical and mechanical
advancements. Designed for professionals as well as clinicians, this microscope offers many features and functions for a
diverse set of applications. Here are a few points highlighting the benefits of the Lx400:
- Extra clarity and contrast is provided through a 360° rotatable Binocular body inclined at 30° with IPD adjustments.
- The pressure die cast stand consists of ball bearing, frictionless sideways focusing to avoid any loss in motion.
- The sturdy new stylish design provides a high degree of comfort as well as stability.
- The high powered objectives are spring loaded to prevent accidental damage to specimen slides.
- The reverse angle quadruple nosepiece has a comfortable ribbed grip for easy rotation that also safeguards the turret
system against any damage. All positions are par-centered and par-focalised ensuring the highest level of accuracy.
- The ball bearing mechanical stage allows smooth travel over a 78 x 54mm area with spring loaded stage clamps for retaining
the specimen at the exact position desired. A 0.1mm vernier scale provides accurate location of specimen area.
- High power illumination is delivered through our well crafted Universal Power Supply and operates with any 100V- 240V AC
input. The LED configuration is operational with an in-built rechargeable battery P/N 9135000-902, which when plugged
in, draws power from a Universal Power Supply operating at 100V-240V Ac input. This ensures continuous operation even
under fluctuating voltages.
- Our Halogen bulb (6V-20W) has an average life span of up to 2,000 hours. Our LED has an average life span of up to
100,000 hours.
- The Lx 400 comes equipped with a removable N.A. 1.25 Abbe condenser with aspheric lens for brighter illumination levels,
and an iris diaphragm for better resolution and contrast control.
INTRODUCTION
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1. After the microscope has been used for observation of a specimen
containing bacteria, clean all parts coming in contact with the specimen
to prevent infection.
¡ Be sure to remove the specimen before moving this product.
¡ In case the specimen is damaged by erroneous operation, it is important
to clean all surfaces that may have come in contact with the specimen.
2 To avoid potential electrical hazards when replacing either halogen bulb
or LED, turn the microscopes main switch to the OFF position and
disconnect power cord from wall outlet in advance. Whenever you
replace your microscope bulb, allow lamp socket and bulb to cool before
touching (Fig. 1)
Applicable bulb/LED replacement: 6V20W Halogen bulb P/N CX-013; 6V30W Halogen bulb P/N EL-455
or LED P/N 9135000-901
3. Install microscope on a sturdy, level table or bench and avoid any restriction of air vents in the base of the unit.
Do not place microscope on a flexible surface, as this could result in blocking the air vents and cause overheating.
4. Always use the power cord provided by LABOMED. If the proper power cord is not used, product safety
performance cannot be warranted.
5. When installing the microscope, route the power cord away from the microscope frame. Should the power cord
come in contact with the microscopes base, the power cord could melt due to overexposure to heat.
6. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If
the unit is not grounded, LABOMED can not warrant electrical safety.
7. Never allow metallic objects to penetrate the air vents of the microscope frame as this could result in user injury and
damage to the microscope.
8. After operation of microscope, be sure to disconnect power cord from connector socket of the microscope or from
the wall power outlet.
Fig. 1
Safety Symbols
Symbol Explanation
This surface has a tendency to heat up and should not be touched unless
system has completely cooled down.
Before use, carefully read the instruction manual. Improper use could result in
injury to the user and/or damage to the equipment.
Main switch is ON.
Warning against risk of electric shock.
Main switch is OFF.
The following symbols are found on the microscope. For optimal use, it is recommended that users understand
these symbols and always use the equipment as prescribed.
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SAFETY INFORMATION
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Lx 400
1. A microscope is a precision instrument with delicate glass components.
Please handle with care.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature, humidity, dust and vibrations. (For the operating
conditions, see chapter 14, "SPECIFICATIONS")
3. The microscope is ventilated by natural convection. Be sure to leave
enough space (10 cm or more) around body when installing the unit.
4. Arm handle is provided for carrying the microscope.
To prevent damage, do not hold the microscope by the stage or
observation tube.
Be sure to remove the specimen from the stage clip while
transporting unit to avoid damage to the specimen slide.
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Getting Started
1. Clean all glass components by wiping gently with cleaning cloth provided. To remove fingerprints or oil smudges,
wipe with cleaning cloth slightly moistened with a mixture of petroleum (85%) and isopropanol (15%).
Since solvents such as petroleum and isopropanol are highly flammable, they must be handled carefully.
Be sure to keep these chemicals away from open flames or potential sources of electrical sparks - for
example, electrical equipment that is being switched “ON” or “OFF”. Also remember to always use these
chemicals only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To
clean non-glass components, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope as this could result in malfunction or mitigated performance.
4. When not using the microscope, ensure that the frame is fully cooled
before storing the unit in a dry locker or covering with a dust cover
(provided).
5. To clean the condenser/phase turret, fully loosen the securing thumb
screw(1) and remove the condenser then, wipe the front lens of the
condenser with optical cleaning solution (mixture suggested above)
and lens tissue.
The condenser can be re-attached by replacing the condenser in its
seat, tightening securing thumb screw, and raising condenser bracket
to desired position.
6. Be sure to observe your local rules/regulation for product disposal.
Fig. 2
Fig. 3
Maintenance and Storage
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Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may not be warranted. In
addition, the equipment may also suffer damage. Always use the equipments as outlined in this instruction manual.
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Care & Maintenance
Your microscope has been engineered for a long and safe operational life with the least amount of maintenance
required. In general, routine maintenance is limited to keeping the microscopes working parts lubricated and optics
clean. Always cover the microscope with the provided dust cover when not in use.
Optical Cleaning:
1. The objectives have been adjusted for a tight fit to prevent any damage during transportation. To remove an
objective, rotate it counterclockwise while gripping it with a rubber sheet, etc. to avoid any slippage.
2. To clean the lens surfaces, remove dust using a soft brush or compressed air (cans available at your local
electronics store). For removing finger marks or grease, soft cleaning cloth or lens tissue lightly moistened with
cleaning solution (85% petroleum ether and 15% isopropanol) should be used. For cleaning the objective optics,
use Methanol. Observe sufficient caution in handling Methanol. Place the objectives and/or eyepieces on a dust-
free surface (e.g. aluminum foil). All other optical components to be cleaned should be as accessible as possible.
3.. Blow all loose dust particles away with compressed air or mini dust blower.
4. Remove all water-soluble dirt with distilled water. If this is unsuccessful repeat using a solution of diluted hand
soap liquid. Remove any remaining residue with a dry cotton swab.
5. To remove oil, use a solution of diluted hand-soap liquid initially. If this does not produce a satisfactory result,
repeat the cleaning using a solvent (Optical Cleaning Solution 85% petroleum ether and 15% isopropanol).
6. Grease must always be removed using a solvent.
7. Cleaning is achieved by using a spiral motion from the center to the rim. Never wipe using zig-zag movements as
this will only spread the dirt. With larger optical surfaces (e.g. tube lenses) the spiral motion starts initially at the rim
before moving to the middle and is then followed by a center to rim cleaning motion. Normally several spiral wipes
are recommended.
We recommend pure, volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above.
Wipe using a spiral movement. Do not use a zig-zag motion!
2. Cleaning of painted surfaces :
Avoid the use of any organic solvent ( e.g. thinner, xylene, ether, alcohol etc.) for cleaning of painted surfaces of
the instrument. Painted surfaces can be cleaned with a very lightly moistened micro fiber cloth. Loose dust and
other dirt particles can be removed using a soft bristle brush used exclusively for this purpose.
zig-zag motion (X) spiral motion ( )
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Lx 400
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Lx 400 Binocular
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Lx 400
X-Y movement control knob
Binocular viewing tube, 30° inclined
Observation tube
clamping screw
Coarse and
fine focus knob
Mechanical
stage
On/Off intensity regulator
Specimen holder
Revolving nosepiece
Objectives
Eyepieces
Abbe condenser
Filter holder
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Lx 400 Trinocular
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X-Y movement control knob
On/Off intensity regulator
Eyepieces
Revolving nosepiece
Objectives
Specimen holder
Coarse and
fine focus knob
Mechanical
stage
Observation tube
clamping screw
Trinocular viewing tube
Trinocular Port
Abbe condenser
Filter holder
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