Labnet Z400K User Manual

INSTRUCTION MANUALINSTRUCTION MANUAL

INSTRUCTION MANUAL

INSTRUCTION MANUALINSTRUCTION MANUAL

FORFOR

FOR

FORFOR

REFIGERAREFIGERA

REFIGERA

REFIGERAREFIGERA

BENCH BENCH

BENCH

BENCH BENCH

TEDTED

TED

TEDTED

TT

OP CENTRIFUGEOP CENTRIFUGE

T

OP CENTRIFUGE

TT

OP CENTRIFUGEOP CENTRIFUGE

Z 400 KZ 400 K

Z 400 K

Z 400 KZ 400 K

Labnet International, Inc.Labnet International, Inc.

Labnet International, Inc.

Labnet International, Inc.Labnet International, Inc.
31 Mayfield Avenue31 Mayfield Avenue

31 Mayfield Avenue

31 Mayfield Avenue31 Mayfield Avenue
Edison, NJ 08837Edison, NJ 08837

Edison, NJ 08837

Edison, NJ 08837Edison, NJ 08837

Phone: [phone]Phone: [phone]

Phone: [phone]

Phone: [phone]Phone: [phone]
Fax: [phone]Fax: [phone]

Fax: [phone]

Fax: [phone]Fax: [phone]

Z 400 K / March 1997

1 E

INDEXINDEX

INDEX

INDEXINDEX

1.1.

1.

1.1.

........................................................................................................................................................................................................................................................................

....................................................................................................................................

........................................................................................................................................................................................................................................................................

General InformationGeneral Information

General Information

General InformationGeneral Information

1.1 Precautions and hazards .............................................................................. 3 E

1.2 Description .................................................................................................... 4 E

1.3 Safety standards ........................................................................................... 4 E

1.4 Technical data............................................................................................... 5 E

1.5 Accessories supplied with each centrifuge unit............................................ 6 E

1.6 Warranty........................................................................................................ 6 E

2.2.

InstallationInstallation

2.

Installation

2.2.

InstallationInstallation

2.1 Unpacking the centrifuge .............................................................................. 6 E

2.2 Transport ....................................................................................................... 6 E

2.2 Required space ............................................................................................. 6 E

2.3 Installation ..................................................................................................... 7 E

3.3.

How to install and load a rotorHow to install and load a rotor

3.

How to install and load a rotor

3.3.

How to install and load a rotorHow to install and load a rotor

3.1 Mounting and securing a swing out rotor...................................................... 7 E

3.2 Mounting and securing an angle rotor .......................................................... 8 E

3.3 Mounting and securing a hematocrit rotor .................................................... 8 E

3.4 Overloading of rotors..................................................................................... 9 E

3.5 Removing of the rotor.................................................................................... 9 E

4.4.

OperationOperation

4.

Operation

4.4.

OperationOperation

4.1 Power switch ................................................................................................. 10 E

4.2 Lid release..................................................................................................... 10 E

4.3 Lid lock .......................................................................................................... 10 E

4.4 Preselection of speed / RCF-value ............................................................... 11 E

4.5 Preselection of operating time ...................................................................... 12 E

4.6 Preselection of temperature and pre-cooling ................................................ 13 E

4.7 Preselection of brake intensity and acceleration .......................................... 14 E

4.8 Keyboard - Starting the centrifuge - "quick"-key........................................... 15 E

4.9 Key "stop"...................................................................................................... 15 E

5.5.

Temperature FeatureTemperature Feature

5.

Temperature Feature

5.5.

Temperature FeatureTemperature Feature

5.1 Temperature regulation................................................................................. 16 E

6.6.

Safety facilitiesSafety facilities

6.

Safety facilities

6.6.

Safety facilitiesSafety facilities

6.1 Imbalance...................................................................................................... 16 E

6.2 Overtemperature ........................................................................................... 16 E

7.7.

Service and MaintenanceService and Maintenance

7.

Service and Maintenance

7.7.

Service and MaintenanceService and Maintenance

7.1 Service and inspection of the centrifuge ....................................................... 17 E

7.2 Cleaning the centrifuge ................................................................................. 17 E

7.3 Cleaning the centrifuge after breakage of glass tubes/bottles ..................... 18 E

7.4 Disinfection.................................................................................................... 18 E

8.8.

BreakdownBreakdown

8.

Breakdown

8.8.

BreakdownBreakdown

8.1 Emergency lid release ................................................................................... 18 E

8.2 Check list / Trouble shooting......................................................................... 19 E

PagePage

Page

PagePage

2 E

Z 400 K / March 1997

1.1.

General InformationGeneral Information

1.

General Information

1.1.

General InformationGeneral Information

1.1 Precautions and hazards

Before putting the centrifuge into operation, please read this instructionBefore putting the centrifuge into operation, please read this instruction

Before putting the centrifuge into operation, please read this instruction

Before putting the centrifuge into operation, please read this instructionBefore putting the centrifuge into operation, please read this instruction
manual carefully.manual carefully.

manual carefully.

manual carefully.manual carefully.

The centrifuge must not be operated by unqualified persons not familar with the
correct use and intended purpose of the machine. Please use only the original
spare parts.
For personal and enviromental safety, special attention has to be paid to the
following precautions:

The Hermle Centrifuges are neither explosion proof nor inert gas shielded and
should therefore never be operated in explosion-hazardais locations.
Never stay in the safety zone of 30 cm around the centrifuge or deposite
dangerous goods inside this zone during centrifugation.
The centrifugation of the flammable or explosive or radioactive samples is not
allowed.
Further more do not spin samples reacting chemically with each other with high
energy when explosed to air.
Never spin toxical or phatological material without adequate safety precautions i.e.
centrifuging of buckets/tubes without or with defective hermetic sealing is not
allowed.
The enduser is engaged to performe appropriate disinfection procedures in case
dangerous goods have contaminated the centrifuge or its accessories.
The general universal laboratory precautions should be observed in case infection
materials are centrifuged. If necessary, please contact your local security officer!
It is prohibited to run the centrifuge with rotors not suited for this centrifuge model.
Under no circumstances, the lid at the centrifuge should be opened while the rotor
is still turning respectively running with a speed of more than 2 meter per second.

The following rules must be strictly adhered to:The following rules must be strictly adhered to:

The following rules must be strictly adhered to:

The following rules must be strictly adhered to:The following rules must be strictly adhered to:

Do not operate the centrifuge in case it is not installed correctly.
Never operate the centrifuge in a dismounted state (e.g. without covering sheet
metal)
Do not run the centrifuge when electrical or mechanical assembly groups have
been tampered with by unauthorized persons.
Never use accessories such as rotors and buckets which are not exclusively
approved by

Hermle Labortechnik GmbHHermle Labortechnik GmbH

Hermle Labortechnik GmbH, except commercially available

Hermle Labortechnik GmbHHermle Labortechnik GmbH

centrifuge tubes of glas or plastic.
Do not spin corrosive samples which may cause damages in material and impair
the mechanic resistance.
never operate the centrifuge with rotors or buckets which show any signs of
corrosion or mechanical damage

The manufacturer is only responsible for the security and reliability of theThe manufacturer is only responsible for the security and reliability of the

The manufacturer is only responsible for the security and reliability of the

The manufacturer is only responsible for the security and reliability of theThe manufacturer is only responsible for the security and reliability of the
centrifuge if:centrifuge if:

centrifuge if:

centrifuge if:centrifuge if:

The unit is operated according to the instruction manual.
Modifications, repairs and new adjustments are performed by HERMLE authorized
persons and the electrical installation of the location where the centrifuge is
operated corresponds to the IEC-regulations.

Z 400 K / March 1997

3 E

1.2 Description

The Z 400 K is a refigerated universal centrifuge which covers many fields of
applications by offering a wide range of accessories.
The centrifuges are equipped with the Hermle Standart-Control panel.
Speed, RCF - value, running time and temperature are set with easy to use control
knobs.
The precise parameters selected are shown on the large digital LED display.
The refigeration system is CFC free.
By offering a wide range of accessories, the centrifuge covers many fields of
applications.

1.3 Safety standards

The centrifuge corresponds to the general requirements set by German law for
medical apparatus, "MedGV" group 3.
The following standards have been considered for the production of our
centrifuges:

- Accident prevention rules for centrifuges, UVV-VBG 7z.

- Accident prevention rules for electrical equipment & installations, UVV-VBG 4.

- Accident prevention rules for cooling systeme, UVV-VBG 20

- International Standart IEC 1010-1 and IEC 1001-2-D

- European Standart PR EN 61010-1 and PR EN 61010-2-2

- Electrical interference suppression according to interference degree B as
per VDE 0871.

4 E

Z 400 K / March 1997

1.41.4

Technical dataTechnical data

1.4

Technical data

1.41.4

Technical dataTechnical data

______________________________________________________________________

Manufacturer Hermle Labortechnik GmbH

______________________________________________________________________

Type Z 400 K

______________________________________________________________________

Dimensions:
Width 74 cm
Depth 34 cm
Height 58 cm

______________________________________________________________________

Weight 79 kg

______________________________________________________________________

Noise level 60 dB(A)

______________________________________________________________________

Max. speed 13.500 rpm
Max. Volume 1 000 ml
Max. RCF 17310 x g
Admiss. density 1,2 kg/dm

3

Admiss. kinetic energy 3 740 Nm

______________________________________________________________________

Electrical connection 230 V/50 Hz 1Ph 120 V/60 Hz 1Ph
Current 5,6 A 8,0 A
Connected load 1288 Watt 960 Watt

______________________________________________________________________

Interference suppression VDE 0871, interference degree B

______________________________________________________________________

Service dept. at Hermle ## 49 (0) 74 26 / 96 22 55

______________________________________________________________________

Address of service: _________________________________

_________________________________
_________________________________

______________________________________________________________________

Address of agent: _________________________________

_________________________________
_________________________________

Z 400 K / March 1997

5 E

1.5 Accessories supplied with each centrifuge unit
2 Replacement fuses, 1 Instruction manual, 1 Tool for removing the rotor

1.6 Warranty
The centrifuge has been subjected to thorough testing and quality control.
In the unlikely event of any manufacturing faults occuring, the centrifuge and rotors

are covered by warranty for a period of one year from date of delivery.
This warranty becomes invalid in case of wrong operation, use of non-appropriate

spare parts or accessories and non-authorized modification of rotor or centrifuge.

The manufacturer reserves the right for any technical modifications of theThe manufacturer reserves the right for any technical modifications of the

The manufacturer reserves the right for any technical modifications of the

The manufacturer reserves the right for any technical modifications of theThe manufacturer reserves the right for any technical modifications of the
product in respect to technical improvement.product in respect to technical improvement.

product in respect to technical improvement.

product in respect to technical improvement.product in respect to technical improvement.

2.2.

InstallationInstallation

2.

Installation

2.2.

InstallationInstallation

2.1 Unpacking the centrifuge
The centrifuge is supplied in a carton on a pallet. Remove the tightening

straps . Open the carton, take off the packing and remove the carton.
The instruction manual and the accessories mentioned under 1.5 should be kept

with the centrifuge.

2.2 Transport
Avoid impacts during transportation and do not drop the unit to perserve it from

being damaged.

2.3 Required space
The centrifuge should be installed on a rigid, even surface. The Z 400 K should

only be operated on a stable laboratory table/cabinet etc..
Balance the centrifuge with a spirit level.
To guarantee the necessary heat dissipation, the unit has to be placed in that way,
that there is a space of minimum 15 cm on each side of the unit.
Never place the centrifuge in positions subject to excessive heat, e.g., strong
sunlight, as the performance of the unit is based upon an ambient temperature
of +23°C.

6 E

Attention:Attention:

Attention:

Attention:Attention:
The new safety rules require a safety operation cycle of 30 cm around theThe new safety rules require a safety operation cycle of 30 cm around the

The new safety rules require a safety operation cycle of 30 cm around the

The new safety rules require a safety operation cycle of 30 cm around theThe new safety rules require a safety operation cycle of 30 cm around the
centrifuge. Mark this area to indicate that no person and no dangerouscentrifuge. Mark this area to indicate that no person and no dangerous

centrifuge. Mark this area to indicate that no person and no dangerous

centrifuge. Mark this area to indicate that no person and no dangerouscentrifuge. Mark this area to indicate that no person and no dangerous
material (e.g. comprising inflammable or infectious liquids) are presentmaterial (e.g. comprising inflammable or infectious liquids) are present

material (e.g. comprising inflammable or infectious liquids) are present

!!

!

!!

material (e.g. comprising inflammable or infectious liquids) are presentmaterial (e.g. comprising inflammable or infectious liquids) are present
during centrifugation.during centrifugation.

during centrifugation.

during centrifugation.during centrifugation.

Z 400 K / March 1997

2.4 Installation

- Check that the power supply corresponds to that on the manufacturer's rating
label which is mounted on the rear panel, then connect the power cord to the
centrifuge and the socket.

- The line voltage circuit breaher is maximum a 16 Amp. type K slow release for
commonly used instruments.

- That an ermergency switch is installed outside the room to disconnect the power
supply in case of a troubled run

- The power switch is at the rear side of the unit. Plug in the power cord.

- The digital indications on the display are lighting up.

- Press key "lid". You can open the centrifuge lid now (see chapter 4.2).

3.3.

How to install and load a rotorHow to install and load a rotor

3.

How to install and load a rotor

3.3.

How to install and load a rotorHow to install and load a rotor

3.1 Mounting and securing a swing out rotor

Clean the motor shaft, as well as the rotor mounting hole with a piece of cloth and
place the rotor on the motor shaft ensuring that the pins align correctly with the
rotor slots (see sketch 1).

IncorrectIncorrect

Incorrect

IncorrectIncorrect

CorrectCorrect

Correct sketch 1

CorrectCorrect

Hold the rotor with one hand and secure the rotor to the shaft by turning the
rotor nut counter-clockwise.

When loading the buckets and tube racks you should proceed according to
sketch 2. It is very important to load the rotor with the complete set of buckets/tube
racks.

silicone grease.silicone grease.

silicone grease.

silicone grease.silicone grease.

The bucket insert bolts of the rotor should be regularly greased withThe bucket insert bolts of the rotor should be regularly greased with

The bucket insert bolts of the rotor should be regularly greased with

The bucket insert bolts of the rotor should be regularly greased withThe bucket insert bolts of the rotor should be regularly greased with

Fill the tubes equally by eye-measuring and insert them into the tube-holes or tube
racks. The difference in weight between the buckets should not exceed 10 grams.

IncorrectIncorrect

Incorrect

IncorrectIncorrect

Z 400 K / March 1997

CorrectCorrect

Correct sketch 2

CorrectCorrect

7 E

It is also allowed to operate for example a 4-place swing out rotor with 2 loaded and
2 unloaded buckets, but it is important that the loaded buckets are then opposite
each other.

3.2 Mounting and securing an angle rotor

Clean the motor shaft, as well as the rotor mounting hole with a piece of cloth and
place the rotor on the motor shaft ensuring that the pins align correctly with the rotor
slots (see page 7 E / sktech 1).
Hold the rotor with one hand and secure the rotor to the shaft by turning the rotor
nut counter-clockwise.

ATTENTION!ATTENTION!

ATTENTION!

ATTENTION!ATTENTION!

Before operation, secure the rotor lid to the rotor by pressing the snap connector on
the rotor nut. Load the rotor according to sketch 3.
Fill the tubes equally by eye-measuring and insert them into the tube-holes of the
rotor. The difference in weight between the tubes should not exceed 2 - 3 grams.

It is also allowed to operate a 6-place rotor with 2 or 4 loaded tubes only, but it
is important that the 2 or 4 occupied bore-holes are opposite each other (see
figure 1a and 1b). When using tubes without lid in angle rotors please note that the
tubes should only be filled partly i.e. 60-75% of the maximum tube volume.

IncorrectIncorrect

Incorrect

IncorrectIncorrect

3.3 Mounting and securing a hematocrit rotor

Clean the motor shaft and the rotor mounting hole with a piece of cloth and
place the rotor on the motor shaft ensuring that the pins align correctly with
the rotor slots.

Hold the rotor with one hand
and secure the rotor to the
shaft by turning the rotor nut
counter-clockwise.

8 E

CorrectCorrect

Correct sketch 3

CorrectCorrect

Z 400 K / March 1997

When loading the rotor, make sure that the sealing is in goodWhen loading the rotor, make sure that the sealing is in good

When loading the rotor, make sure that the sealing is in good

When loading the rotor, make sure that the sealing is in goodWhen loading the rotor, make sure that the sealing is in good
condition. The rubber should not be brittle, dirty or damaged by thecondition. The rubber should not be brittle, dirty or damaged by the

condition. The rubber should not be brittle, dirty or damaged by the

condition. The rubber should not be brittle, dirty or damaged by thecondition. The rubber should not be brittle, dirty or damaged by the
capillaries. If necessary replace the sealing (order number ofcapillaries. If necessary replace the sealing (order number of

capillaries. If necessary replace the sealing (order number of

capillaries. If necessary replace the sealing (order number ofcapillaries. If necessary replace the sealing (order number of
the sealing : 23-5147).the sealing : 23-5147).

the sealing : 23-5147).

the sealing : 23-5147).the sealing : 23-5147).

To remove or to close the rotor lid press both locking bolts together.

3.4 Overloading of rotor
The max. load permitted for a rotor, which is determined by the manufacturer, as

well as the max. speed allowed with the rotor (see indications on the rotor itself)
must not be exceeded.

The liquids with which the rotors are loaded should have an average homogeneous
density of 1,2 g per ml or less, when the rotor is running at maximum speed.

To spin liquids of a higher density, the speed should be reduced according to the
following formula:

1,21,2

1,2

Reduced speedReduced speed

Reduced speed

Reduced speedReduced speed

n n

n

n n

redred

red

redred

= =

=

= =

higher density valuehigher density value

higher density value

higher density valuehigher density value

1,21,2

x max. speed (n x max. speed (n

x max. speed (n

x max. speed (n x max. speed (n

maxmax

max

maxmax

))

)

))

ExampleExample

Example

ExampleExample

In case of any questions please contact the manufacturer!In case of any questions please contact the manufacturer!

In case of any questions please contact the manufacturer!

In case of any questions please contact the manufacturer!In case of any questions please contact the manufacturer!

::

:

::

3.5 Removing of the rotor
Take off the rotor lid and hold the rotor with one hand. Turn the rotor nut clockwise,

until the rotor is loose and take the rotor vertically off the shaft.

ATTENTION:ATTENTION:

ATTENTION:

ATTENTION:ATTENTION:
Never operate the centrifuge with rotors or buckets which show any signs ofNever operate the centrifuge with rotors or buckets which show any signs of

Never operate the centrifuge with rotors or buckets which show any signs of

Never operate the centrifuge with rotors or buckets which show any signs ofNever operate the centrifuge with rotors or buckets which show any signs of
corrosion or mechanical damage.corrosion or mechanical damage.

corrosion or mechanical damage.

!!

!

!!

corrosion or mechanical damage.corrosion or mechanical damage.
Never operate with strongly corrosive materials which could damage rotorNever operate with strongly corrosive materials which could damage rotor

Never operate with strongly corrosive materials which could damage rotor

Never operate with strongly corrosive materials which could damage rotorNever operate with strongly corrosive materials which could damage rotor
and buckets.and buckets.

and buckets.

and buckets.and buckets.

n n

n

n n

redred

red

redred

= =

=

= =

1,21,2

1,2

1,21,2
1,71,7

1,7

1,71,7

x 4000 = 3360 rpmx 4000 = 3360 rpm

x 4000 = 3360 rpm

x 4000 = 3360 rpmx 4000 = 3360 rpm

Z 400 K / March 1997

9 E

4.4.

OperationOperation

4.

Operation

4.4.

OperationOperation

4.1 Power switch

4.2 Lid release

Connect the cord plug to the appropriate wall socket.
After connecting the digital displays will light up.
The control panel is equipped with a stand by function.

When the green control lamp (1) of the key "lid" (2) lights, the rotor stands still and
the centrifuge lid is ready to open.
Press key "lid" (see figure 5) to open the lid. The green control lamp will extinguish
as soon as the lid will be opened or the unit started.

1 Control lamp (rotor is stationary):
Shows that the lid is closed correctly.

2 Key "lid".
To open the centrifuge lid

4.3 Lid lock

Close the centrifuge lid, after the rotor has been fixed correctly as described.
The centrifuge can only be started when the lid is closed correctly.
The green control lamp of the key "lid" will light as soon as the lid is closed
correctly.
When the rotor starts accelerating the control lamp of the key "lid" extinguishes
and the lid cannot be opened.

Figure 5

10 E

Z 400 K / March 1997

4.4 Preselection of speed / RCF

You can preset the speed between 250 rpm amd 13500 rpm and RCF-value
between 500 and 18000 x g.

3 1 2 6 4 5 figure 6

When the centrifuge lid is open, you can preset the required speed or rcf with the
knob(2).
If the centrifuge lid is closed and during the run, the speed can be changed as
follows:
Press key "preset" (1), hold it and at the same time change speed with the knob
(2).
The preselected speed will be indicated in the speed display (3).
With the key "speed/rcf" (6) you can change indication from speed to rcf-value.
The two green control lamps (4 or 5) show which mode is active.
The centrifuge will accelerate only to the max. allowed speed of the inserted rotor.
The allowed max. speed for all rotors please see table under.

Z 400 KZ 400 K

Z 400 K

Z 400 KZ 400 K

Rotor max. Speed Rotor max. Speed

220.27 V02 4 000 rpm* 220.92 V01 13 000 rpm

220.59 V06 13 500 rpm 220.93 V01 3 500 rpm

220.50 V05 3 500 rpm 220.81 V05 3 500 rpm

220.86 V01 3 500 rpm 220.96 V02 6 000 rpm

220.88 V01 13 500 rpm 220.97 V02 6 000 rpm

220.89 V06 12 000 rpm 221.02 V01 3 500 rpm

221.03 V01 3 500 rpm

*with rectangular bucket 610.015 only 3500 rpm

Z 400 K / March 1997

11 E

4.5 Preselection of operating time
You can adjust the desired operating time between 1 and 60 minutes or hold.

With the centrifuge lid open you can preset the operating time with the knob (3).
During the run and with the centrifuge lid closed you have to press additionally key
"preset" (1), hold it and change operating time by turning the knob (3).
The preselected running time will be indicated on the time display (5).

At the end of a run the preset operating time will be kept for further runs.

continuous run
press start

2 3 1 4

For continuous runs turn the knob clockwise to the limit stop. The continuous run will
be indicated on the digital indication with two minus signs "--".
You can check the operating time during a continuous run as the follows:

- Press the botton (4) start.

After this the operating time will be automatically in the follow sequence
indicated:
For example 4 hours and 13 minutes operating time:

min/sec. min/sec. min/sec.

symbol "h" for hours number of hours symbol "m" for

min/sec.

number of minutes

minutes

min/sec.min/sec.

running time
indication end ­continuous run

Attention: to check the operating time you have to wait one minute after start
You can stop a continuous run with key "stop".

12 E

Z 400 K / March 1997

4.6 Preselection of temperature and pre-cooling

Pre-cooling:Pre-cooling:

Pre-cooling:

Pre-cooling:Pre-cooling:

To avoid considerable temperature deviations at the beginning of an operation at
low temperatures, pre-cool the centrifuge together with the rotor before insertion
of the samples.

For pre-cooling please proceed as under:

1. Insert a rotor into the centrifuge and secure it correctly to the motor shaft.

2. Set the desired working temperature.

3. To shorten the pre-cooling process, set a speed which is about 20% of the
maximum speed indicated for the rotor.

4. Start the centrifuge.

5. Within 10-15 minutes the centrifuge should reach the selected temperature.

Preselection of temperature:Preselection of temperature:

Preselection of temperature:

Preselection of temperature:Preselection of temperature:

321

With the centrifuge lid open, the temperature can be set with the knob (1).
With the centrifuge lid closed or during a run you have to press additionally key
"preset" (3) to change or set the temperature.
The temperature can be preselected from -10°C up to +40°C.
With open lid the temperture display shows the preset temperature and with a
closed lid or during a run the display shows the actual rotor temperature.

Z 400 K / March 1997

13 E

4.7 Preselection of brake intensity and acceleration

With key "fast/slow" (1) you have the choice between two steps of brake and
acceleration intensity.

1 2 3

If the green control lamp "fast" (2) is lighting, the unit accelerates and decelerates
fast.
If the green control lamp "slow" (3) is lighting, the unit accelerates and decelerates
very softly.

Figure 8

preset
speed

fast

slow

Acceleration and deceleration time of all rotors

rotor deceleration time acceleration time

in sec. in sec.
fast slow fast slow

220.27 V02 35 6 9 43 50

220.59 V06 25 4 5 16 35

220.81 V05 38 8 8 40 71

220.86 V01 62 1 25 66 8 6

220.88 V01 33 5 7 23 37

220.89 V01 23 3 7 18 32
220,92 V01 30 5 7 21 37

220.93 V01 62 1 35 58 8 8

220.96 V02 34 9 0 28 63

220.97 V02 33 8 9 28 62

221.02 V01 34 6 0 34 60

221.03 V01 33 6 3 28 56

14 E

Z 400 K / March 1997

4.8 Keyboard - Starting the centrifuge - "quick"-key

1 Key "lid": to open the centrifuge lid. If the green LED
2 of the key "lid" is lighting the lid is closed correctly.

3 Key "quick": for short time runs

4 Key "start": to start the centrifuge.

5 Key "stop": to stop the centrifuge before the preset

operating time has expired or to stop the centrifuge
at continuous run.

Figure 6

Starting the centrifugeStarting the centrifuge

Starting the centrifuge

Starting the centrifugeStarting the centrifuge

The rotor has to be fixed correctly and completely loaded (see point 3).
Close the centrifuge lid. As soon as the green LED of the key "lid" is lighting the
centrifuge can be started. Therefore press key "start".

"quick"-key - Short time runs
For short centrifuge runs you can start the run with the key "quick". Press the
key "quick". The centrifuge starts and keeps running as long as you press the
"quick"-key. The operating time will be indicated in seconds on the digital
indication "time".

4.9 Key "stop"
Press the key "stop" if you want to interrupt a centrifuge run. The centrifuge

decelerates according to the adjusted brake intensity.

Z 400 K / March 1997

15 E

5.5.

Temperature FeaturesTemperature Features

5.

Temperature Features

5.5.

Temperature FeaturesTemperature Features

5.1 Temperature regulation
The temperature regulation with sensor, refrigeration system and processor control

system is spread in a way that the adjusted temperature gets reached within the
shortest possible time. This time depends on room temperature, temperature
preselection, type of rotor and speed. During this regulation time deviations on the
temperature indicator above or below the adjusted temperature may occure.
Nevertheless rotor as well as samples get cooled continuously at the fixed value.

If you are working with temperature sensitive samples a pre cooling of rotor,
eventually buckets and racks to corresponding temperature is necessary (see pre­cooling 4.6.)

temperature

Temperature
display

Temperature samples

6.6.

Safety facilitiesSafety facilities

6.

Safety facilities

6.6.

Safety facilitiesSafety facilities

6.1 Imbalance

In case of unequal loading of opposite buckets/tube racks or tube-holes, the
operation will be interrupted during the acceleration phase. The rotor will be
decelerated to standstill. Additionally the error message "ERROR" appears on the
preset display "speed".

If the actual display "speed" shows error no. 1, the difference in weight of the
samples is too big. Fill the tubes and load the rotor as described under point 3.

If the actual display "speed" shows error no. 2, there can be several reasons for:

- The imbalance switch is not adjusted correctly.

- The imbalance switch is defective.

6.2 Overtemperature

The overtemperature control system protects your samples as well as the centrifuge.
If the temperature in the rotor chamber of the centrifuge exceeds +50°C the preset

!!

!

!!

display "speed" will indicate "ERROR". At the same time error no. 10 will be
indicated on the actual display "speed". When the rotor is stationary, open the lid of
the centrifuge and let the temperature in the rotor chamber cool down to +30°C.

time

16 E

Then re-start the centrifuge.

Should the same failure occur again, please call our service.Should the same failure occur again, please call our service.

Should the same failure occur again, please call our service.

Should the same failure occur again, please call our service.Should the same failure occur again, please call our service.

Z 400 K / March 1997

7.7.

Service and MaintenanceService and Maintenance

7.

Service and Maintenance

7.7.

Service and MaintenanceService and Maintenance

7.1 Service and inspection of the centrifuge

Centrifuge service and inspection should be done regularly and only by authorized
and qualified personnel.
Use only original spare parts!

7.2 Maintenance and cleaning
Maintenance

The maintenance of the centrifuge confines esentially to keeping clean the rotor
chamber, the rotor and the accessories as well as to the regular lubrication at the
rotor inserts bolts of the swing out rotor.
Vaseline, available in nearly each store, is the most suited lubricat. However,
please ensure that the vaseline has to be free of resin and acid. The use of
lubricants containing molykote and graphite is not allowed.
Please pay special attention to anodized aluminium parts. Breakage of rotors can
be caused even by slight damages.
In case the rotor, buckets or tube racks get in touch with corrosive liquids, the
respective spots and parts have to be cleaned carefully.
Corrosive liquids are for example:

- alkaline soup-solution

- alkaline amino

- strong acids

- solutions containg heavy metals

- waterfree and chlorinated solvents

- salt solutions e.g. sea-water
Cleaning:
The purpose of a thorough cleaning is, beside hygienical reasons, the avoidance of

corrosion by soiling. In order to avoid the damage of anodized parts such as rotors,
reduction plates ect., only neutral cleaning agents with a ph-value 6-8 should be
used.
Never use an alkaline cleaning agent (ph > 8). After cleaning please ensure that
all parts are dried thoroughly by hand or in a warm-air-cabinet
(max. temperature +50°C).

It is recommended that all anodized aluminum parts are regularly treated with anti
corrosion oil, so that their durability will be increased and the corrosion risk
reduced.

By humidity and non hermetically closed samples, condensate (mist) may be
formed. The condensate (mist) has to be removed regulary with a cloth from the
rotor chamber.

Z 400 K / March 1997

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7.3 Cleaning of centrifuge after breakage of glass tubes/glass bottles
With high g-values, there is a possibility that tube breakage will occur. Should this

happen, the centrifuge, rotor, buckets, adapters and the rotor chamber must be
thoroughly cleaned and all broken particles removed immediately.

If this is not done, they could scratch the protective coating of the rotor.
If the rotor chamber has not been properly cleaned, this will produce a fine black

dust which can cause significant damage to the centrifuge chamber, rotor, buckets
and the samples.

7.4 Disinfection
If, for example through tube breakage, infectious material is spilled into the

centrifuge, the rotor, rotor chamber, buckets etc. should be disinfected !!!

Rotor and swing out buckets must not be autoclaved.Rotor and swing out buckets must not be autoclaved.

Rotor and swing out buckets must not be autoclaved.

Rotor and swing out buckets must not be autoclaved.Rotor and swing out buckets must not be autoclaved.

Rotor and rotor chamber should then be treated with a neutral disinfection agent,
for example on formalin basis. A disinfectant spray should be used to thoroughly
clean the rotor chamber, rotor, bucket, tube rack etc..

8.8.

BreakdownBreakdown

8.

Breakdown

8.8.

BreakdownBreakdown

8.1 Emergency lid release

In case of power failure or any malfunction, the lid can be opened manually to
protect your samples.
Please proceed as follows:

- Switch off the centrifuge and unplug the power cord.

- There is a plastic plug at the left side of the centrifuge housing. Behind that

plastic plug there is a red cord.

- Remove the plastic plug and pull the red cord.

The lid can then be opened (see figure 15).

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figure 15

Z 400 K / March 1997

8.2 Check list / Trouble shooting

The error message will be indicated by a certain number on the digital speed
display. At the same time "ERROR" appears on the preset display.

There is a distinction between two different kinds of errors. The digits on the
indication "speed" have the following meaning:

Error no. 1 - 49 (Forced stop)
If one of those errors occurs, the rotor will be braked from the preset speed to 0.
As soon as the rotor has stopped, the error message can be reset by opening and
closing the centrifuge lid.

Error no. 50 - 99 (Emergency stop)
If this occurs, the frequency converter will be switched off. This means that the
rotor will be stopped brakeless. To reset the error message you have to plug off
and plug in the power cord.

If the unit stops due to an error indication you should restart the unit to check if the
error occurs again.

The error numbers which are not listed in this chapter are not in use at the time of
publication and they are reserved for future use in widening the error recognition
program.

Error no.: 1Error no.: 1

Error no.: 1

Error no.: 1Error no.: 1

ImbalanceImbalance

Imbalance

ImbalanceImbalance

Reason: Incorrect loading of the rotor

Action: Balance your samples

Reason: Incorrect adjustment of the imbalance switch

Action: Imbalance switch has to be readjusted (call service)

Error no.: 2Error no.: 2

Error no.: 2

Error no.: 2Error no.: 2

Permanent imbalance signalPermanent imbalance signal

Permanent imbalance signal

Permanent imbalance signalPermanent imbalance signal

Reason: Position of the imbalance switch not correct

Action: Imbalance switch has to be readjusted (call service)

Reason: Imbalance switch is defective

Action: Imbalance switch has to be replaced (call service)

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Error no.:Error no.:

Error no.:

Error no.:Error no.:

1010

Overtemperature in the rotor chamber (more than + 50 ° C)Overtemperature in the rotor chamber (more than + 50 ° C)

10

Overtemperature in the rotor chamber (more than + 50 ° C)

1010

Overtemperature in the rotor chamber (more than + 50 ° C)Overtemperature in the rotor chamber (more than + 50 ° C)

Reason: Breakdown of the refrigeration system / Electronic failure

Action: Call service

Reason: Temperature sensor defective / Electronic failure

Action: Call service

Error no.: 11Error no.: 11

Error no.: 11

Error no.: 11Error no.: 11

Temperature sensorTemperature sensor

Temperature sensor

Temperature sensorTemperature sensor

Reason: Short circuit at the temperature sensor or at the sensor cable

Action: Call service

Reason: Chamber temperature is too low, below - 25°C

Action: Solenoid valve is not working. Call service

Error no.: 20Error no.: 20

Error no.: 20

Error no.: 20Error no.: 20

No rotor identificationNo rotor identification

No rotor identification

No rotor identificationNo rotor identification

Reason: No rotor inserted

Action: Insert rotor into the unit

Reason: Rotor identification sensor defective

Action: Call service

Reason: Inserted rotor has no indicator ring

Action: Use a correct rotor

Reason: Rotor is not fixed correctly to the motor shaft

Action: Insert rotor correctly. The pins have to align correctly with the

rotor slots (see chapter 3)

Error no.: 21Error no.: 21

Error no.: 21

Error no.: 21Error no.: 21

Start Bit is missingStart Bit is missing

Start Bit is missing

Start Bit is missingStart Bit is missing

Reason: A magnet of the indicator ring is missing

Action: Check indicator ring and call service

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Z 400 K / March 1997

Error no.: 22Error no.: 22

Error no.: 22

Error no.: 22Error no.: 22

Rotor is not mentioned in the rotor chartRotor is not mentioned in the rotor chart

Rotor is not mentioned in the rotor chart

Rotor is not mentioned in the rotor chartRotor is not mentioned in the rotor chart

Reason: Rotor is not authorized for this unit

Action: Insert only rotors authorized for this unit

Error no.: 25Error no.: 25

Error no.: 25

Error no.: 25Error no.: 25

Power failurePower failure

Power failure

Power failurePower failure

Reason: Power failure while rotor is in motion

Action: Open and reclose the lid, restart the centrifuge

Error no.: 30Error no.: 30

Error no.: 30

Error no.: 30Error no.: 30

Radius correctionRadius correction

Radius correction

Radius correctionRadius correction

Reason: Radius correction value too big

Action: Adjust the radius correction to the correct value

Error no.: 36Error no.: 36

Error no.: 36

Error no.: 36Error no.: 36

Relay for the frequency converter cannot be releasedRelay for the frequency converter cannot be released

Relay for the frequency converter cannot be released

Relay for the frequency converter cannot be releasedRelay for the frequency converter cannot be released

Reason: Defect on the power board

Action: Call service

Error no.:50,51Error no.:50,51

Error no.:50,51

Error no.:50,51Error no.:50,51

Memory failureMemory failure

Memory failure

Memory failureMemory failure

Reason: Internal or external memory failure

Action: Restart the unit, if the failure occurs again, call service

Error no.: 55Error no.: 55

Error no.: 55

Error no.: 55Error no.: 55

OverspeedOverspeed

Overspeed

OverspeedOverspeed

Reason: Overspeed sensor or engine speed sensor defective

Action: Call service

Error no.: 60Error no.: 60

Error no.: 60

Error no.: 60Error no.: 60

Engine speed sensor signal is missingEngine speed sensor signal is missing

Engine speed sensor signal is missing

Engine speed sensor signal is missingEngine speed sensor signal is missing

Reason: Engine speed sensor defective or parting of a cable at the sensor

Action: Call service

Z 400 K / March 1997

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Error no.: 70Error no.: 70

Error no.: 70

Error no.: 70Error no.: 70

Interface of the frequency converterInterface of the frequency converter

Interface of the frequency converter

Interface of the frequency converterInterface of the frequency converter

Reason: Communication of controller, power board, interface cable

and frequency converter is not working

Action: Call service

Error no.: 82-83Error no.: 82-83

Error no.: 82-83

Error no.: 82-83Error no.: 82-83

Cutoff power board - frequency converterCutoff power board - frequency converter

Cutoff power board - frequency converter

Cutoff power board - frequency converterCutoff power board - frequency converter

Reason: Overcurrent or undervoltage due to power supply fluctuations

Action: Restart the unit, take care that the power supply is stable

Error no.: 84Error no.: 84

Error no.: 84

Error no.: 84Error no.: 84

Overtemperature at the drivingOvertemperature at the driving

Overtemperature at the driving

Overtemperature at the drivingOvertemperature at the driving

Reason: Temperature at the converter or motor too high

Action: Switch off the centrifuge. Wait for about 15 min. and switch

the unit on again

Error no.: 85-87Error no.: 85-87

Error no.: 85-87

Error no.: 85-87Error no.: 85-87

FailuresFailures

Failures

FailuresFailures

Reason: Internal defect

Action: Call service

Error no.: 90Error no.: 90

Error no.: 90

Error no.: 90Error no.: 90

Emergency lid releaseEmergency lid release

Emergency lid release

Emergency lid releaseEmergency lid release

Reason: The centrifuge lid has been opened by the emergency lid release

during the run

Action: Close centrifuge lid. Danger of accident!

Reason: Control switch of the lid lock is defective

Action: Call service

Error no.: 94Error no.: 94

Error no.: 94

Error no.: 94Error no.: 94

Voltage loss during runVoltage loss during run

Voltage loss during run

Voltage loss during runVoltage loss during run

Reason: The power supply is below the tolerance for a short moment

Action: Wait till stillstand of the rotor. Open centrifuge lid after the yellow

LED "lid" is lighting. Switch off and on the main switch.

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Z 400 K / March 1997