LABEC V-18 User Manual
INSTRUCTION MANUAL
Laboratory Equipment Pty Ltd
email: sales@labec.com.au
Ph: 02 9560 2 811 • Fax: 02 9560 61 3 1
www.labec.com.au
2014
INSTRUCTION MANUAL FOR
SPECTRO- UV/V-16/18
UV/VIS SPECTROPHOTOMETERS
PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION
Coooontents
Safety ….…………………………………………………………………………. 1111
General ………………………………………………………………………. 1
Electrical …………………………………………………………………….. 1
Warning ………………………………………………………………………… 1
Working Principle ………………………………………………………………2
Unpacking Instructions …………………………………………………… 2
Specifications ……………………………………………..……………… 2
Installation …………………………………………………………………… 3
Operation ………………………………………………………..……………… 4
Prepare the Spectrophotometer ………………………………………… 4
Description of keys ………… …………………………………… 5
Turn on spectrophotometer … …………………………………………… 5
Basic operation…………………………………………………………… 6
Analyse Sample …………………………………………………………… 8
Basic Mode …………………………………………………………….. 8
Quantitative ….………………………………………………………..… 9
kinetics …….………………………………………………………………… 16
Utility ……………………………………………………………………… 21
Maintenance……………………………………………………………………. 27
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Safety:
The safety statements in this manual comply with the requirements of the HEALTH AND
SAFETY AT WORK ACT, 1974.
Read the following before installing and using the instrument and its accessories. The
63(&752UV16/18 should be operated by appropriate laboratory technicians.
General:
The apparatus described in this manual is designed to be used by properly trained
personnel in a suitable equipped laboratory. For the correct and safe use of this
apparatus it is essential that laboratory personnel follow generally accepted safe
procedures in addition to the safety precautions called for in this manual.
The covers on this instrument may be removed for servicing. However, the inside of the
power supply unit is a hazardous area and its cover should not be removed under any
circumstances. There are no serviceable components inside this power supply unit.
For 63(&752 UV
ome of the chemicals used in spectrophotometer are corrosive and/or inflammable and
S
samples may be radioactive, toxic, or potentially infective. Care should be taken to
follow the normal laboratory procedures for handling chemicals and samples.
16/18, avoid touching the high voltage power supply at all times.
Electrical:
Before switching on the apparatus, make sure it is set to the voltage of the local power
supply (see Fig.1-1).
Fig. 1-1
The power cord shall be inserted in a socket provided with a protective earth contact.
The protective action must not be negated by the use of an extension cord without a
protective conductor.
Voltage Switch
Warning:
Any interruption of the protective conductor inside or outside the apparatus or
disconn
ection of the protective earth terminal is likely to make the apparatus dangerous.
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0
Intentional interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall be made
inoperative and be secured against any unintended operation.
NEVER touch or handle the power supply on63(&752UV-16
The protection is likely to be impaired if, for example, the apparatus
Shows visible damage
Fails to perform the intended measurements
Has been subjected to prolonged storage under unfavorable conditions
Has been subjected to severe transport stresses
/18 due to the high voltage.
Working Principle:
The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps
to supply the light; 2) A Mono-chromator to isolate the wavelength of interest
and eliminate the unwanted second order radiation; 3) A sample compartment
to accommodate the sample solution; 4) A detector to receive the transmitted
light and convert it to an electrical signal; and 5) A digital display to indicate
absorbance or transmittance. The block diagram (Fig 1-2) below illustrates
the relationship between these parts.
Block diagram for the Spectrophotometer
100%T
Light Mono- Sample Detector Display
Source chromator Compartment
Fig1-2
In your spectrophotometer, light from the lamp is focused on the entrance slit of the
monochromator where the collimating mirror directs the beam onto the grating. The
grating disperses the light beam to produce the spectrum, a portion of which is focused on
the exit slit of the monochromator by a collimating mirror. From here the beam is passed
to a sample compartment through one of the filters, which helps to eliminate unwanted
second order radiation from the diffraction grating. Upon leaving the sample
compartment, the beam is passed to the silicon photodiode detector and causes the
detector to produce an electrical signal that is displayed on the digital display.
Unpacking Instructions:
Carefully unpack the contents and check the materials against the following packing list to
ensure
that you have received everything in good condition.
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Packing List
Description Quantity
Spectrophotometer............................................................... 1
Mains Lead........................................................................... 1
Cuvettes................................................................................ Set of 4, glass
.......................................................................... Set of 2, quartz
Manual.................................................................................. 1
Note: the qurtz cuvette will not be included in the visible instruments.
Specifications:
Model
Wavelength Range 320-1100nm 190-1100nm
Spectral Bandwidth 4nm 2nm 4nm 2nm
Optical System Single Beam, Grating 1200lines/mm
Wavelength Accuracy ±0.5nm
Wavelength Repeatability 0.3nm
Wavelength Resolution ±0.1nm
Photometric Accuracy ±0.3%T
Photometric Repeatability ±0.2%T
Photometric Range -0.3-3A, 0-200%T
Stray Light 0.05%T@360nm 0.05%T@220nm,340nm
Stability ±0.002A/h @500nm
Display Graphic LCD (128X64 dots)
Keyboard 22 Membrane keypad
Photometric Mode T, A, E
Detector Si Photodiode
Sample Compartment
Light Source Tungsten lamp Tungsten & Deuterium
Output USB Port Parallel Port (printer)
Power Requirement AC 220V/50Hz or 110V/60Hz
Dimensions (W x D x H) 470 x 370 x 180mm
Weight 12kg 14kg
63(&752V16 63(&752V18
Standard 10mm path length cuvette
Accommodates 100mm path length cuvette with optional holder
63(&752UV16 SPECTRO-UV18
Installation:
1. After carefully unpacking the contents, check the materials with the packing list
(page 2) to ensure that you have received everything in good condition.
2. Place the instrument (Fig.1-3) in a suitable
location away from direct sunlight. In order to have the best performance from
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your instrument, keep it as far as possible from any strong magnetic or electrical
fields or any electrical device that may generate high-frequency fields. Set the
unit up in an area that is free of dust, corrosive gases and strong vibrations.
Compartment
Control Panel
Holder Changer Knob
Fig.1-3
3. Remove any obstructions or materials that could hinder the flow of air under and
around the instrument.
4. Use the appropriate power cord and plug into a grounded outlet.
Fan
Parallel Port USB Port
Fig.1-4
5. Turn on your
SPECTRO-UV16/18 model spectrophotometer. Allow it to warm up for
20 minutes before taking any readings. We suggest you then do the Calibrate
System with the Search 656.1nm to set the wavelength to the deuterium lamp
emission line.
NOTE:
This symbol means Caution, Risk of Danger.
Power Cord Inlet
Fuse
Power Switch
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Operation:
Prepare the spectrophotometer
Fig 1-2 is the control panel. User can perform all operations by pressing the keys and all
the results and operation information are displayed on the LCD.
Fig 2
Description of keys
【START/STOP】 Start/stop testing
【RETURN】 Exit to previous screen or cancel the operation;
【ENTER】 Confirm the inputted data or selected item;
【CLEAR】 Clear all characters when you are inputting or clear test results display
on the screen;
【SET】 Parameter set
【GOTOλλλλ】 Set wavelength;
【ZERO】 Blank
【PRINT】 Print test results
【0】-【9】 Input number or menu select.
【.】 Input dot;
【-】 Input minus symbol;
【】,【】 Scroll items for selecting; Browse the items for selection;
Turn on spectrophotometer
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Turn on spectrophotometer by pressing the Power Switch (IO). The instrument starts to
initiate and the steps are as below:
1.The instrument will position filter first, then initialize lamp position and AD converter,
then D2 / W lamps warm up, then Wavelength reset and get dark current, After initializing
printer, checking battery and setting up system, the screen display the main menu. And
the self-testing process display as fig 3.
2 .If the data in memory has been lost, the instrument will directly calibrate system
without any choice for you.
Note: DO NOT OPEN SAMPLE COMPARTMENT LID DURING SELF-TESTING.
√Self-testing
●
●Filter √
●●
○
○Lamp √
○○
○
○AD √
○○
√Self-testing
○
○D2 Lamp √
○○
○
○W Lamp √
○○
○
○Reset WL √
○○
√Self-testing
○
○System √
○○
√Self-testing
○
○Dark Current √
○○
○
○Printer √
○○
○
○Battery √
○○
●
●Basic Mode
●●
○
○Quantitative
○○
○
○Kinetics 08:00
○○
○
○Utility 01/01
○○
Main Menu
Fig 3
Basic operation
Blank
Push the blank cuvette into the light path.
Press the key 【ZERO】for blanking
Note:1
Set Wavelength (Example: set wavelength in “Basic mode”)
、、、、
If the reference solution is too thick, the energy will be low.
、
.Blanking is automatic after a wavelength change.
2
DO NOT OPEN SAMPLE COMPARTMENT LID DURING BLANKING.
Press【GOTOλλλλ】(Fig 4).
Basic Mode：：：：
GOTO
λλλλ
Basic Mode：：：：
WL=546.0nm
0.000 Abs
546.0 nm
RETURN
Please Input New
WL=
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Fig 4
Use numeric keypad to input wavelength (e.g. 500nm).
Press 【ENTER】 to change the wavelength, and then blank; After blanking,
the screen displays as the left one of Fig 5.
Note:1. If you don’t want to change the wavelength, you can press 【RETURN】
2. the wavelength range is 190-1100, other figure is invalid.
Basic Mode：：：：
0.000 Abs
START
500.0nm 0.000Abs
No. WL Abs
1 500.0 0 .000
RETURN
500.0 nm
Fig. 5
Measurement (Example: set wavelength in “Basic mode”)
Push the blank cuvette into the light path. Then press 【ZERO】
】】】...
.
Press 【START/STOP】in the left one of Fig. 5 to go into testing
interface.(The right one of Fig 5)
Press 【START/STOP】again in the right one of Fig. 5, the testing
result displays on the screen, repeat this step, the results will
display on the screen one by one.
Print test report
(For example: Print the report in “Basic mode”, the right one of Fig. 5)
Press【PRINT】in the right one of Fig.5 to print the report (Fig. 6)
Basic Mode
NO. Abs. T%
1 0.000 100.0%
2 0.000 100.0%
Fig 6
Note: After printing, all the data displayed on the screen will disappear.
Before measurement
Make a blank reference solution by filling a clean cuvette (or test tube) half full with
distilled or de-ionized water or other specified solvent. Wipe the cuvette with tissue
to remove the fingerprints and droplets of liquid. Fit the blank cuvette into the 4-cell
linear changer and place the cuvette in the slot nearest you. For the UV16\18,
push the changer so that the cuvette is in the light path (Push the rod in). Close the
lid.
】】】...
.
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