Labcyte Echo 650, Echo 655, Echo 655T, Echo 650T Quick Start Manual

LABCYTE INC.

SITE PREP GUIDE | Echo® 650 Series Liquid Handler

Echo® 650 Series Liquid Handler
(Models: 650, 655, 650T, 655T)

SITE PREP GUIDE

Server Version 3.1 | FEBRUARY 2019

This guide provides site preparation information for the Echo® 650 Series Liquid Handler. This includes models 650, 655, 650T
and 655T. The topics below describe the site preparation requirements and instrument specifications for the Echo Liquid
Handlers.

Pre-Installation Requirements

l Site Requirements
l User Supplied Equipment Requirements
l Communication Requirement s
l Accommodating Your Echo Liquid Handler
l Noise Ratings
l Power Ratings

What You Will Be Receiving

l Shipping Dimensions
l Echo Liquid Handler Components
l Chiller
l Junair Compressor (Optional and sold separately by Labcyte)
l Vacuum Pump (Optional and sold separately by Labcyte)
l Client PC (Sold separately by Labcyte)

Pre-Installation Requirements Verification Form

© 2019 LABCYTE INC. All rights reserved.

QSG-2019-1

FEB 2019

Operating environment

Temperature: 21°C ± 5°C (70°F± 9°F)

Relative humidity: 10-80%, non-condensing for the Echo Liquid Handler and 35-80% for the
SMC Chiller

ACPower (2 options)

Option 1: 100-120 V, 50/60 Hz, > 10A

Option 2: 200-240 V, 50/60 Hz, > 5A

A minimum of two electrical sockets within 183 cm (6 feet) of the Echo Liquid Handler
supporting a minimum of 800 W are required.

The Echo Liquid Handler and SMC chiller maximum power consumption is 600W and 500W
respectively.

Up to four additional electrical sockets are required for the client PC, monitor, vacuum pump
(optional) and air compressor (optional).

Compressed air source

Clean, filtered, oil-free, dry air at 552 kPa (80 PSI) minimum, 1034 kPa (150 PSI) maximum

Maximum consumption by Echo Liquid Ha ndler does not exceed 1 cfm at 150 PSI

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Pre-Installation Requirements

The pre-installation requirement s are described in the topics below:

l Site Requirements
l User Supplied Equipment Requirements
l Communication Requirement s
l Accommodating Your Echo Liquid Handler
l Noise Ratings
l Power Ratings

Site Requirements

The following site requirements must be met before the Echo Liquid Handler can be installed:

Note: The Echo Liquid Handler power configuration is factory set and requires a Field Service visit in order to be changed
on site.

Note: The Junair OF302-4B oil-less compressor can be purchased from Labcyte (Part #001-12164). For more information,
see the Junair Compressor (Optional and sold separately by Labcyte) topic.

2

Vacuum source for source
plate dryer (2 options)

Option 1: House supply. Minimum 200 Torr (266 mbar, 22 in Hg Vac) minimum pumping speed

8.0 m3/hr

Option 2: Standalone vacuum with surge tank (10 L). 100 Torr (133 mbar, 26 in Hg Vac),
minimum pumping speed 2.0 m3/hr

Instrument mounting surface
(minimal requirements)

The Echo Liquid Handler can emit vibration while in operation and is susceptible to vibration
from other devices. For this reason, the Echo system should be placed on a surface that is
isolated from surrounding equipment or designed to suppress the transmission of vibration
from devices. The surface must be sturdy, level, and water-resistant with cross-bracing or
welded joints.

The surface must be ca pable of supporting a minimum of 142 kg (313 lbs) weight with
sufficient surface area to accommodate the Echo Liquid Handler in the recommended
operating envelope (see Echo Liquid Handler Dimensions). If the mounting surface is a table
top, the table legs should not rest on ca sters. If the table has casters, they should be locked.

If the chiller will be installed on the bench surface, it must accommodate an additional 14 kg
(31 lbs).

Destination plate samples

At installation, ensure common destination plates to be used with the instrument are
available for tuning the destination plate gripper.

Coupling Fluid

Use only ASTM D-1193 Type 1 water in the fluidics system

Coupling Fluid Temperature: 22.1°C ± 0.9°C (71.8°F± 1.6°F)

Hardware

Compressed air fitting compatible with 0.170 in ID / 0.250 in OD tubing between the compressed
air supply a nd the Echo Liquid Handler tubing (Labcyte provides the compressed air line and
fitting with the Echo Liquid Handler)

Vacuum fitting that connects 3/8 in ID, 5/8 in OD tubing to either house vacuum or a vacuum
pump. The supplied vacuum line is HercoBraid, 3/8 in ID, 5/8 in OD (RyanHerco P/N 0512-072)

Client PC

Minimum Requirements: Microsoft® Windows® 7 using 32- or 64-bit OS, or Microsoft® Windows®
10 64-bit; Intel® Core™ 2 Duo processor or later; 1GB of RAM or greater; 1 GB of a vailable disk
storage; display resolution of 1280 x 1024 (1920x1020 preferred); 2 x Gigabit ethernet ports

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Note: A standalone Vacuubrand MZ 2S-NT vacuum system can be purchased from Labcyte (Part #001-8104). For more
information, see the Vacuum Pump (Optional and sold separately by Labcyte) topic.

Note: The 10 L surge tank comes standard with the Echo Liquid Handler to ensure consistent vacuum.

User Supplied Equipment Requirements

The user must provide the following items:

Note: Client PC can be purchased directly from Labcyte (Part #ECHO-Client Computer). For information on the Client PC
available from Labcyte, see the Client PC (Sold separately by Labcyte) topic.

3

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Note: If you are integrating the Echo Liquid Handler into an automated system and need to install the Client + Server
software onto a workstation, it must cont ain an Intel Core™ i5 processor or better.

Note: If you are setting up an Echo 650T or 655T Liquid Handler that will be using tubes, a capper/decapper is required
before Labcyte Service and Support can install or service the instrument.

4

Server connection Echo Liquid Handling Platform (Server Version 3.1.x)

Network connection 10/100/1000 BaseT

Network protocol TCP/IP

User account

Labcyte (The client PC must have an account, with administrator privileges, that is accessible by
Labcyte Service a nd Support to service the Echo Liquid Handler system.)

The Echo Liquid Handler
Dimensions

53.8 cm width x 74.6 cm depth x 91.5 cm height (21.2 x 29.3 x 36.0 inches)

Echo Liquid Handler working
envelope (minimal installation
requirements)

Top: It is recommended to have 25.4 cm (10 in) above the instrument to remove the top cover
for servicing.

Front: It is recommended to have 40.6 cm (16 in) to a llow sufficient clearance to load and
unload microplates by hand or by robot.

Side: It is recommended to have 121.9 cm (48 in) minimum on left and right sides to allow for
serviceability. The Echo Liquid Handler should have sufficient space to be rotated, otherwise

it will need to be removable for service.

Rear: It is recommended to have 30.5 cm (12 in) minimum behind the instrument to allow for
cables, hoses, tubing, and maintenance access. The coupling fluid bottle and optional caddy
fit within the rear clearance but can be relocated if necessary.

SMCChiller Dimensions

30 cm plus fittings width x 34 cm Depth x 18 cm Height (11.8 in x 13.4 in x 36.4 in). It is
recommended that the chiller be installed within 121.9 cm (48 inches) of the Echo Liquid
Handler.

Echo Client PC Additional space for the client PC, monitor, keyboard a nd mouse.

Other

Space must be alloca ted for:

l Any robotics in the system
l Workspace for microplates

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Communication Requirements

The user environment must support the following communication requirements:

Accommodating Your Echo Liquid Handler

The user must set aside the space to accommodate Echo Liquid Handler installation.

Note: The instrument can be installed with a minimum of 2.5 cm (1 in) at the top if there is sufficient space to lift the top
and slide it all the way forward. Talk to your Labcyte service representative for additional details.

5

Noise Rating 64 dBA

Power Rating 600W without the chiller

Heat Rating 600W without the chiller

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Figure 1: Instrument Layout (Front View, Top View, Side View)

Noise Ratings

The noise ratings for the Echo Liquid Handler are below:

Power Ratings

The power ratings for the Echo Liquid Handler are below:

6

Instrument shipping crate weight 278 kg (577 lbs)

Instrument shipping crate
dimensions

114.3 cm width x 99.1 cm depth x 124.8 cm height (45 x 39 x 49.1 inches)

Accessory crates

Additional devices and/or consumables purchased together with the Echo Liquid
Handler may ship in the crate with the Echo Liquid Handler if there is sufficient room or
separately.

Vacuum line

HercoBraid, 9.5 mm (3/8 in) ID, 9.5 mm (5/8 in) OD (RyanHerco P/N 0512-072)

HFCD 17612 coupling body (Colder Products)

Vacuum Surge Tank
Assembly

(Optional) Usage dependent on configuration, but included with the Echo (P/N 001-5150)

Compressed air line

4.6 meters (15 ft) of polyurethane tubing; 6.35 mm (0.25 in) OD, 4.24 mm (0.170 in) ID (SMC
Pneumatics P/N TIUB07-BU)

LCD 13004 coupling body (Colder Products).

Coupling fluid bottle 1 L (P/N 001-5438)

Accessory kit Includes tubing a nd power cord for connecting the chiller (P/N 001-15272)

SMC chiller P/N 001-11149

Ethernet cable Crossover, 3 meters (10 ft)

Software Echo Client Utility installer provided on CD or USB drive

Voltage Frequency Power Fuse Part Number

100-120 V 50 Hz 8 A 250 VAC, 10 A, FAST 001-3966

200–240 V 50 Hz 5 A 250 VAC, 5 A, FAST 001-3967

Table 1: Electrical Configurations for the Echo Liquid Handler

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

What You Will Be Receiving

Shipping Dimensions

The Echo Liquid Handler System is shipped in a crate with t he following dimensions:

Echo Liquid Handler Components

The following components are included with the Echo Liquid Handler:

AC Power, Configuration, and Fuses

The following table describes the AC Power and Fuse requirements for the Echo Liquid Handler:

Note: The power cord may need to be replaced with one conforming to local AC power receptacles. To determine which
AC power configuration you have, see t he Power and Fuse Rating Label on the back panel of the Echo Liquid Handler.

7

Power Supply

The SMC chiller uses an auto switching power supply capable of running any single-phase
AC voltage in the range 100-240V, 50-60 Hz

A power cord suitable for the install location is provided

Maximum power is 500W

Temperature Control

The SMC chiller controls the temperature to +/- 0.02 °C by cooling and heating as
appropriate

The SMC chiller is air cooled

Ambient Operating Conditions

Temperature: 10-35 °C

Relative Humidity: 35-80%

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Chiller

The SMC chiller (P/N 001-11149) is included with your Echo system. The SMC chiller utilizes a stainless steel thermoelectric (Peltier)
heat exchanger that regulates fluid temperature. The SMC chiller ships configured for operation with Echo Liquid Handlers:

Figure 2: SMCChiller

8

Voltage V 100 100 120 200 200 230 230 3x400 3x440

Frequency Hz 50 60 60 50 60 50 60 50 60

Motor

HP 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60

kW 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44

Displacement

I/min 108 138 138 108 138 108 138 108 138

CFM 3.81 4.87 4.87 3.81 4.87 3.81 4.87 3.81 4.87

FAD @ 8 bar

l/min 38 44 44 38 44 38 44 38 44

CFM 1.34 1.55 1.55 1.34 1.55 1.34 1.55 1.34 1.55

Maximum
pressure

bar 8 8 8 8 8 8 8 8 8

psi 120 120 120 120 120 120 120 120 120

Maximum current A 7.5 8.7 6.6 4.1 4.6 3.4 4.1 1.4 1.4

Tank size

liters 4 4 4 4 4 4 4 4 4

gallon 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1

Weight

kg 22 22 22 22 22 22 22 22 22

lbs 49 49 49 49 49 49 49 49 49

Noise level

dB
(A)/lm

65 66 66 65 66 65 66 65 66

Thermal
protection

Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes

Duty cycle 100% 100% 100% 100% 100% 100% 100% 100% 100% 100%

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Junair Compressor (Optional and sold separately by Labcyte)

The Echo Liquid Handler requires a compressed air source that provides clean, filtered, oil-free, dry air at 552 kPa (80 PSI) minimum
and 1034 kPa (150 PSI) maximum. The Junair OF302-4B oil-less compressor can be purchased from Labcyte (Part # varies
dependent on geography.)

The user must also provide a fitting between the compressed air supply and the Echo Liquid Handler. Labcyte provides the
compressed air line and fitting with the Echo Liquid Handler, but the customer must provide a source compatible with 170 in
ID/.250 in OD tubing.

The table below lists the specifications for the Junair OF302-4B compressor:

Note: Maximum consumption by Echo Liquid Handler for instrument pneumatics does not exceed 1 cfm at 150 PSI.

9

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Figure 3: Junair OF302-4B oil-less compressor

10

House supply

Minimum 200 Torr (266 mbar, 22 in Hg Vac) minimum pumping speed 8.0
m3/hr

Standalone vacuum with surge tank (10 L) 100 Torr (133 mbar, 26 in Hg Vac), minimum pumping speed 2.0 m3/hr

Permissible a mbient temperature
storage/operation

14 to 140/50 - 104 F°

-10 to +60/ +10 - +40 C°

Permissible relative atmospheric moisture during
operation (no condensation)

30 to 85%

No-load speed 50/60 hZ 1500/1800 rpm

Device fuse Slow blow fuse 6.3 A

Maximum pumping speed 50/60 Hz

1.3/1.4 cfm

2.2/2.4 m3/h

Ultimate vacuum (absolute)

5.2 Torr
7 mbar

Maximum permissible outlet pressure (absolute)

29 psi
2 bar

Maximum permissible different between inlet and
outlet

29 psi
2 bar

Rated motor power

0.24 hp

0.18 kW

Maximum permissible range of supply voltage
(+10%)

100-115 V ~50/60 Hz
120 V ~ 60 Hz
230 V ~50/60 Hz

Dual voltage motor

100-115 V ~50/60 Hz
120 V ~ 60 Hz
200- 230 V ~50/60 Hz

Maximum rated current at:
100-115 V ~50/60 Hz, 120 V ~ 60 Hz
200- 230 V ~50/60 Hz, 230 V ~50/60 Hz

3 A

1.8 A

Inlet

Hose nozzle for tubing I.D. 3/8 in
Hose nozzle DN 10 mm

Outlet Silencer / G1/4 in

Dimensions L X W X H (approximate)

9.6 X 9.4 X 7.8 in
243 X 239 X 198 mm

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Vacuum Pump (Optional and sold separately by Labcyte)

The Echo Liquid Handler requires a vacuum source. The vacuum dries source plates before they are unloaded. This requirement
can be met through a house vacuum or a vacuum pump.

The vacuum source should have the following specifications:

Customers must provide a fitting that connects the vacuum source and the Echo Liquid Handler vacuum line. The supplied
vacuum line is HercoBraid, 9.5 mm (3/8 in) ID, 9.5 mm (5/8 in) OD (RyanHerco P/N 0512-072).

A standalone Vacuubrand MZ 2S-NT vacuum system can be purchased from Labcyte (Part #001-8104). This vacuum is a dual
voltage/cycle compatible system.

The table below lists the specifications for the Vacuubrand MZ 2S-NT vacuum system:

11

Weight (approximate)

24.2 lbs
11 kg

Operating system Microsoft® Windows 7 32-bit OS

CPU Intel® Core™ XE2 i5-4570S 2.9 GHz Processor

Memory 4GB RAM or greater

Hard drive storage 1 TB hard drive

Video Resolution 1280 x 1024 resolution

Ethernet 2 Gigabit 10/100/1000 (Two Ethernet Cards)

SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 0 01-16480

Figure 4: Vacuubrand MZ 2S-NT vacuum system

Client PC (Sold separately by Labcyte)

Client PC specifications (Dell OptiPlex XE2):

Included Accessories:

l Microsoft® Office 2016
l Monitor, 22 in, DVI-D/DP/VGA
l Dell USB Keyboard, Black
l Dell MS111 USB Optical Mouse, OptiPlex and Fixed Precision
l Labcyte Mousepad

Software specifications:

Optionally, the following Echo software applications are available for purchase:

l Echo Plate Reformat
l Echo Dose-Response
l Echo Cherry Pick
l Echo Array Maker
l Echo Plate Audit
l Echo Combination Screen

Note: Echo 650 Series Liquid Handlers require Echo software applications version 1.7 or higher.

12

Verified Requirement Description Yes No

Operating environment

Are the temperature and relative humidity operating environment
requirements met?

Sturdy, level, water-resistant
bench top

Is there a sturdy, level, water-resistant bench available for the Echo
6XX series instrument?

Operating space envelope

Does the space a vailable for the instrument meet the operating
space envelope requirement?

AC power

Is AC power meeting the AC power specifications and located within
6 feet of the installation location?

Compressed air source

Is there a source of compressed air meeting the compressed air
source specification?

Vacuum source Is there a vacuum source meeting the vacuum specification?

Client PC

Is there a client PC available for the installation meeting the client
PC specification?

Destination plates

Are commonly used destination plates available for tuning the
destination plate gripper at installation?

LABCYTE INC.

SITE PREP GUIDE | Echo® 650 Series Liquid Handler

Pre-Installation Requirements Verification Form

Please send this Pre-Installation Requirement Verification Form to the local Labcyte representative assigned to the installation.
Verification of the pre-installation requirements is required before the service representative will schedule the installation.

One installation visit is provided with the purchase of an Echo 650 Series Liquid Handler instrument. If the
pre-installation requirements are not met at the time of installation, it may delay the installation and require an additional visit.

If you have any questions regarding the lab environment setup or required equipment, please contact your local Labcyte
Representative or email [e-mail].

Echo® Liquid H andlers, Applications Software and Automation SystemsfromLabcyte Inc. are c overed by one or more of the following patents:

United States: 6,416,164; 6,548,308; 6,603,118;6,612,686;6,642,061; 6,666,541; 6,707,038; 6,710,335; 6,746,104; 6,802,593; 6,808,934; 6,809,315; 6,849,423; 6,855,925;6,869,551;

6,893,836; 6,893,115; 6,916,083; 6,932,097; 6,938,987; 6,938,995; 6,991,917; 7,070,260; 7,090,333; 7,185,969; 7,270,986; 7,354,141; 7,405,072; 7,405,395; 7,439,048; 7,454,958;

7,481,511; 7,717,544; 7,899,645; 7,900,505; 7,901,039;8,107,319, 8,389,295; 8,503,266; 8,770,69; 9,212,250; 9,586,215; 9,908,133; 10,112,212;10,118,186. EuropeanPatent EP: 1322430;

1324823;1337325; 1352112; 1366356; 1348116, 1409254, 1534526; 1585636; 1879697.Japan: 4189964; 4309131; 4434581; 4559218; 4624644;4990476; 4955901; 4990476; 5031875.

Additional patents are approvedand pendingin the UnitedStates and other countries.

All product names and brands are properties of their respective owners.

© 2019 Labcyte Inc. All rights reserved. Labcyte®, Echo®, MicroClime®, the Labcyte logo, andAccess™ are

registeredtrademarks ortrademarks ofLabcyte Inc., in the U.S. and/or other c ountries.

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

LABCYTE INC.

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San Jose, CA 95134, USA

Toll-free: + 1 877 742−6548

Fax:+ 1 408 747−2010

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QSG-2019-1

FEB 2019