Labconco Labconco-3849920-rev-e-technical-manual Logic+ and Cell Logic+ A2 | B2 Biosafety Cabinets Axiom C1 Biosafety Cabinets PuriCare Procedure Stations Technical Manual

Logic+® and Cell Logic+® A2 | B2 Biosafety Cabinets

Axiom® C1 Biosafety Cabinets

PuriCare® Procedure Stations

Register this product

LABCONCO CORPORATION

8811 Prospect Avenue
Kansas City, MO 64132
[phone] I [phone]

labconco.com

Please read user’s manual before

operating equipment

Original Instructions

Technical Manual

2

Logic+® Type A2 Biosafety Cabinets

30231xxx1 30241xxx1 30251xxx1 30261xxx1
30238xxx1 30248xxx1 30258xxx1 30268xxx1

30242xxx1 30252xxx1 30262xxx1

PuriCare® Procedure Stations

31241xxx1 31261xxx1 31242xxx1 31252xxx1 31262xxx1

Logic+® Type B2 Biosafety Cabinets

30348xxx1 30368xxx1

Cell Logic+® Type A2 Biosafety Cabinets

32239xxx1 33239xxx1 34239xxx1
32249xxx1 33249xxx1 34249xxx1
32259xxx1 33259xxx1 34259xxx1
32269xxx1 33269xxx1 34269xxx1

Cell Logic+® Type B2 Biosafety Cabinets

32348xxx1 33348xxx1 34348xxx1
32368xxx1 33368xxx1 34368xxx1

Axiom® Type C1 Biosafety Cabinets

30441xxx1 30448xxx1 30461xxx1 30468xxx1

3

Copyright © 2021 Labconco Corporation. The information contained in this manual and the accompanying products are copyrighted and all rights reserved by
Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change.

Warranty

Labconco Corporation provides a warranty to the original buyer for the repair or replacement of parts and reasonable labor as a result of normal and proper use of
the equipment with compatible chemicals. Broken glassware and maintenance items, such as filters, gaskets, light bulbs, finishes and lubrication are not
warranted. Excluded from warranty are products with improper installation, erratic electrical or utility supply, unauthorized repair, use with incompatible chemicals,
or non-factory modifications to the original product.

Purifier® Logic®+ and AxiomTM Biological Safety Cabinets and PuriCare® Procedure Stations carry a five-year warranty from date of installation or six years from
date of shipment from Labconco, whichever is sooner. Warranty is non-transferable and only applies to the owner (organization) of record.

Buyer is exclusively responsible for the set-up, installation, verification, decontamination or calibration of equipment. This limited warranty covers parts and labor,
but not transportation and insurance charges. If the failure is determined to be covered under this warranty, the dealer or Labconco Corporation will authorize
repair or replacement of all defective parts to restore the unit to operation. Repairs may be completed by 3rd party service agents approved by Labconco
Corporation. Labconco Corporation reserves the rights to limit this warranty based on a service agent’s travel, working hours, the site’s entry restrictions and
unobstructed access to serviceable components of the product.

Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This warranty is exclusive and in lieu of
all other warranties whether oral, or implied.

Returned or Damaged Goods

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be accepted. If your shipment was damaged in transit, you must
file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damages.

The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Limitation of Liability

The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of
this equipment are required to become familiar with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water, land,
or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.

For additional questions or support:
Labconco Customer Care [phone]
Labconco Technical Support [phone]

Hours 7:30 a.m.-5:30 p.m. CST

Part #3849920 Rev. E

ECO M149

4

Table of Contents

1: INTRODUCTION 11

Contacting Labconco 11

About This Manual 12

Theory of Operation 12

Type A2 12
Type B2 13
Type C1 – A mode 13
Type C1 – B mode 14

2: SAFETY PRECAUTIONS 15

Typographical Conventions 15

General Safety Precautions 17

Safety Precautions for this Product 19

3: CATALOG NUMBER CONFIGURATOR 21
4: SERIAL TAG 22

Serial Tag Location 22

Serial Tag Information 23

Primary Serial Tag 23
Secondary Serial Tag 23

5: FACTORY TEST REPORT 24

Test Report Location 24

Test Report Information 25

6: ADDITIONAL PRODUCT INFORMATION 26

Data Plate Location 26

5

Data Plate Information 27

7: EXHAUST SYSTEM CONNECTIONS 28

Exhaust Connection Best Practices 28

Air-Tight Damper 30

Exhaust System Requirements 31

Type A2 Models 31
Cell Logic+ Type A2 Models 32
Type B2 Models 32
Type C1 Models 33

8: CONFIGURATION 34

Current Configuration 34

Change Configuration 36

9: CALIBRATION 40

Certifier Password 40

Adjusting Blower Speed 41

Adjusting the Internal Air Damper 43

Setting the Filter Life Gauge 45

Type A2 (8-inch through 10-inch sash) and Type B2 45
Type A2 (12-inch sash) and Type C1 46

Airflow Sensor Calibration 47

Type A2 and Type C1 47
Type B2 48
Zero Point Calibration 50

Vacuum Start Switch Calibration (Type C1 only) 51

10: CERTIFICATION 53

Certifier Password 53

Type A2 HEPA Filter Leak Test Preparation 53

Theoretical Upstream Concentration 54
Upstream Concentration Sampling 54

6

Type A2 Supply HEPA Filter Leak Test 56

Acceptance Criteria 57

Type A2 Exhaust HEPA Filter Leak Test 58

Acceptance Criteria 59

Type B2 Supply HEPA Filter Leak Test Preparation 60

Theoretical Upstream Concentration 60
Upstream Concentration Sampling 60

Type B2 Supply HEPA Filter Leak Test 62

Acceptance Criteria 63

Type B2 Exhaust HEPA Filter Leak Test Preparation 64

Theoretical Upstream Concentration 64

Type B2 Exhaust HEPA Filter Leak Test 65

Acceptance Criteria 65

Type C1 Supply HEPA Filter Leak Test Preparation 66

Theoretical Upstream Concentration 66
Upstream Concentration Sampling 66

Type C1 Supply HEPA Filter Leak Test 68

Acceptance Criteria 69

Type C1 Exhaust HEPA Filter Leak Test Preparation 70

Theoretical Upstream Concentration 70

Type C1 Exhaust HEPA Filter Leak Test 72

Acceptance Criteria 73

Downflow Velocity 74

Acceptance Criteria 75

Inflow Velocity 76

Primary Method 76
Acceptance Criteria 78
Secondary Method 79
Acceptance Criteria 83

Work Area Air Cleanliness Test (optional) 84

Acceptance Criteria 84

Lighting Test (optional) 85

Acceptance Criteria 85

Noise Test (optional) 86

Acceptance Criteria 86

Vibration Test (optional) 86

Acceptance Criteria 86

7

UV Light Intensity Test (optional) 87

Acceptance Criteria 87

Ground Fault Circuit Interrupter (GFCI) Test 88

Acceptance Criteria 88

11: QUICKCHARTS 89

QuickChart – Type A2 3-ft & 4-ft 90

QuickChart – Type A2 5-ft & 6-ft 91

Type A2 Footnotes 92

QuickChart – Type B2 93

Type B2 Footnotes 94

QuickChart – Type C1 95

Type C1 Footnotes 96

12: ADVANCED SERVICE PROCEDURES 97

Tools 97

Required 97
Optional 97

Removal of External Dress Panels 98

Dress Panel 98
Side Panels 99

Front Sash 100

Sash Level Adjustment 100
Sash Sensor Adjustment or Replacement 101

HEPA Filter Replacement 104

Type A2 (8, 9, 10-inch sash) 104
Type A2 (12-inch sash) 107
Type B2 110
Type C1 114

Blower/Motor Replacement 117

Type A2 (8, 9, 10-inch sash) & B2 117
Type A2 (12-inch sash) & C1 Supply Blower/Motor 121
Type A2 (12-inch sash) & C1 Exhaust Blower/Motor 124

Changing the LED Lamps 129

LED Lamp Socket Cap Replacement 131

Changing the Optional UV Lamp 132

8

UV Lamp Socket Replacement 133

Type B2 Prefilter Replacement 134

Air-Tight Damper Adjustment 135

Preparing the Biosafety Cabinet for Gaseous Sterilization 136

Service Fixture Installation & Service Connection 137

13: ELECTRICAL COMPONENTS 140

Electronics Module 140

Electronic Module Components 141
Resetting a Circuit Breaker 142
Power Supply Board 143

Display Board 144

Speaker 145

Blower Motor 146

Wiring Diagrams 148

100-115V 148
208-240V 149

14: TROUBLESHOOTING 150

Blower and Lights not working 150

Blower only will not start 151

Lights only will not illuminate 152

UV Light will not illuminate 153

Airflow Alert activating 154

Filter Life Gauge not at 100% when new 155

Contamination in the work area 156

15: DIAGNOSTICS 157

Sash Sensors 157

Keypad 158

9

Airflow Sensor (optional) 159

Canopy (optional) 160

16: BMS DATA OUTPUT CONNECTION 161

Connection Type 161

Data Output Format 162

Data Output Rate 162

Data Output String Definition 163

Data Output Software Requirements 164

17: VENTUSTM CANOPY CONNECTION KIT 165

Kit Catalog Numbers 166

Installation Procedure 166

Exhaust Damper Installation Procedure 167

Canopy Switch Wiring Connection 168

Calibrate for Operation 169

18: AIRFLOW SENSOR KIT 171

Installation – Type A2 without Ventus Canopy 171

Installation – Type A2 with Ventus Canopy 173

Connection to Electronics Module 175

Configuration (new install only) 176

Calibration 178

19: UV LAMP KIT 181

Kit Catalog Numbers 181

Installation 181

Configuration 183

10

20: IV BAR KIT 185

Kit Catalog Numbers 185

Installation 185

21: PREFILTER KIT 187

Kit Catalog Numbers (Type A2 and B2 only) 187

Installation 187

Attach Prefilter(s) to Towel Catch – G4 Paper 188
Attach Prefilter(s) to Towel Catch – Aluminum Washable 189

22: EN ALARM/RELAY CONTACT KIT 190

Installation 190

Example 1 – Blower Relay Contact (Single Phase AC) 194
Example 2 – Blower Relay Contact (Three Phase AC) 195

23: PARTS LIST 196

Type A2 Consumables 196

Type B2 Consumables 197

Type C1 Consumables 198

Advanced Service Components 199

11

1: Introduction

This text is designed for biosafety cabinet certifiers and servicers. Labconco has
compiled this information to use in the certification or servicing of our Logic+, Cell
Logic+ and Axiom biosafety cabinets, and Puricare procedure stations.

As always, we at Labconco want to assist you in a better understanding of our products
and their operation; if you have any questions, or need additional information, please
contact us.

Thank you for all your support in the past, and in the future.

Contacting Labconco

LABCONCO Corporation
8811 Prospect Avenue
Kansas City, MO 64132
USA

Our hours of operation are from 7:30 am - 5:30 pm CST, Monday through Friday,
except national holidays

We can be reached at the following numbers:
1 [phone] [phone]

Should you require technical assistance, service parts, or have general questions
regarding the product, please direct them to our Product Service Department, so that
your call can be properly routed and answered.

12

About This Manual

This manual contains technical information for all types and models of Labconco
biosafety cabinets and procedure stations. Type A2 biosafety cabinets with a 12 inch
sash operating height, and C1 biosafety cabinets utilize two internal blowers, and Type
A2 and B2 biosafety cabinets with sash operating heights of 8, 9, or 10 inch utilize one
internal blower. Where information is applicable only to Type A2 12 inch or Type C1
models, a color-coded bar is provided to alert model-specific information. Below are
examples of this color-coded bar.

Theory of Operation

This section offers a brief overview of the operation of Type A2, B2, and C1 biosafety
cabinets.

Type A2

All Logic+ Type A2 biosafety
cabinets meet the airflow
requirements of ANSI/NSF Standard

49. Approximately 55-60% of the air
in the cabinet is filtered and
recirculated, while 40-45% is filtered
and exhausted. All Type A2 Logic+
biosafety cabinets feature
intrinsically safe designs, jacketing
contaminated positive pressure
areas with negative pressure zones.

Figure 1-1

Type A2 12

-inch

and C1

51

CURRENT / AVG = 1103 / 1104

NEW FILTER / MAX = 994 / 1450

REV. D

EXHAUST

Type

C1

RECIRCULATE (A MODE)

EXHAUST (B MODE)

13

Type B2

Type C1 – A mode

Figure 1-2

All Logic+ Type B2 biosafety
cabinets meet the airflow
requirements of ANSI/NSF Standard

49. All of the air in the cabinet is
filtered and none is recirculated. All
Type B2 Logic+ biosafety cabinets
feature intrinsically safe designs,
with all contaminated areas under
negative pressure.

Figure 1-3

All Logic+ Type C1 biosafety
cabinets meet the airflow
requirements of ANSI/NSF Standard

49. Approximately 55-60% of the air
in the cabinet is filtered and
recirculated, while 40-45% is filtered
and exhausted back to the lab. All
Type C1 Logic+ biosafety cabinets
feature intrinsically safe designs,
jacketing contaminated positive
pressure areas with negative
pressure zones.

14

Type C1 – B mode

Figure 1-4

All Logic+ Type C1 biosafety
cabinets meet the airflow
requirements of ANSI/NSF Standard

49. The Chem-ZoneTM (center work
surface) air is filtered and exhausted
via a remote building exhaust
system, while the inflow and outer
work surface air is filtered and
recirculated. All Type C1 Logic+
biosafety cabinets feature
intrinsically safe designs, jacketing
contaminated positive pressure
areas with negative pressure zones.

15

2: Safety Precautions

Before unpacking, installing, operating, maintaining, or servicing this equipment, read
the following safety warnings and precautions.

Avant le déballage, l’installation, le fonctionnement, l’entretien ou la maintenance de cet

équipement, lire les avertissements de sécurité et les précautions d’emploi.

CAUTION – See Manual. When this symbol is on the equipment, it indicates a

caution that is detailed in this manual.

MISE EN GARDE – Voir le manuel. Lorsque ce symbole est apposé sur

l’équipement, il renvoie à une mise en garde détaillée dans ce manuel.

Typographical Conventions

DANGER – An imminently hazardous situation which, if not avoided, will result in

death or serious injury.

DANGER – Situation dangereuse imminente qui, si elle n’est pas évitée, peut

entraîner la mort ou des blessures graves.

CAUTION – Indicates a potentially hazardous situation which, if not avoided,

may result in minor or moderate injury or damage to property.

MISE EN GARDE – Signale une situation potentiellement dangereuse qui, si elle

n’est pas évitée, peut provoquer des blessures mineures à modérées ou des

dommages matériels.

NOTE – Advice or suggestions to help the process.
REMARQUE – Conseils ou suggestions pour le déroulement du processus.

16

BURN RISK (HIGH TEMPERATURE) – Air or components that will be very hot.

Take care not to touch these defined areas. Failure to avoid these areas may
result in moderate to severe injury.

RISQUE DE BRÛLURE (TEMPÉRATURE ÉLEVÉE) – Air ambiant ou

composant devenant très chaud. Veiller à ne pas toucher ces zones délimitées.

L’absence de précaution pour éviter ces zones peut entraîner des blessures

modérées, voire graves.

EXTREME COLD (LOW TEMPERATURE) – Air or components that will be very

COLD. Take care not to touch these defined areas. Failure to avoid these areas
may result in moderate to severe injury.

FROID INTENSE (TEMPÉRATURE BASSE) – Air ambiant ou composant

devenant très froid. Veiller à ne pas toucher ces zones délimitées. L’absence de
précaution pour éviter ces zones peut entraîner des blessures modérées voire
graves.

PINCH POINT – Areas or components that can pinch or cut. Take care not to

touch these defined areas.

POINT DE PINCEMENT – Zones ou composants présentant un risque de

pincement ou de coupure. Veiller à ne pas toucher ces zones délimitées.

MOVING PARTS – Areas or components that contain moving parts. Take care

not to touch these defined areas.

PIÈCES MOBILES – Zones ou composants contenant des pièces mobiles.

Veiller à ne pas toucher ces zones délimitées.

RISK OF ELECTRICAL SHOCK – The specified procedure or area poses a risk

of electrical shock. ALWAYS disconnect main power cord or electrical supply
before proceeding.

RISQUE DE CHOC ÉLECTRIQUE – La procédure ou la zone spécifiée présente

un risque de choc électrique. TOUJOURS débrancher le cordon d’alimentation

secteur ou l’alimentation électrique avant toute intervention.

FLAMMABLE / NO SOLVENTS – Do not place flammable liquids or solvents in

this product.

INFLAMMABLE / PAS DE SOLVANTS – Ne placez aucun liquid inflammable

dans cette produit.

17

LIFTING HAZARD – Do not lift or move this equipment without assistance.
DANGER DE LEVAGE – Ne pas soulever ou déplacer cet équipement sans

assistance.

MAGNETIC FIELD IN USE – Magnets or magnetic field present.
CHAMP MAGNETIQUE UTILISE – Présence d'aimants ou de champ

magnétique.

DO NOT TOUCH – Components or areas indicated are sensitive and will suffer

damage if touched. Take care not to touch these defined components or areas.
Failure to avoid these areas will result in damage to the product.

NE PAS TOUCHER – Les composants ou les zones indiquées sont sensibles et

subiront des dégâts s’ils sont touchés. Veiller à ne pas toucher ces composants

ou zones délimité(e)s. L’absence de précaution pour éviter ces zones

endommagera le produit.

TOOL REQUIRED – Tool required to access specified area.
OUTIL NÉCESSAIRE – Outil nécessaire pour accéder à la zone spécifiée.

General Safety Precautions

Follow all the safety precautions described in this section.

Before removing any panels which require a tool for removal, ALWAYS
disconnect the main power cord or electrical supply. Failure to remove all
electrical power before proceeding will result in moderate to serious injury, death,
or damage to property.

Avant le retrait d’un panneau nécessitant l’utilisation d’un outil, TOUJOURS

débrancher le cordon d’alimentation secteur ou l’alimentation électrique. Le non-

respect de la consigne consistant à couper complètement l’alimentation

électrique avant toute intervention peut entraîner des blessures graves, la mort
ou des dommages matériels.

18

Never contact moving parts with your person. Failure to avoid moving parts will
result in moderate to serious injury, death, or damage to property.
Ne jamais toucher les parties mobiles. Le non-respect de la consigne consistant
à éviter les pièces mobiles peut entraîner des blessures graves, la mort ou des
dommages matériels.

Never misuse this product. Never disable, override, or otherwise bypass safety
guards, panels, switches, sensors or alarms. Doing so will result in moderate to
serious injury, death, or damage to this product or property.
Ne jamais utiliser ce produit à mauvais escient. Ne jamais désactiver, annuler ou
contourner les capots, panneaux, interrupteurs, capteurs ou alarmes de sécurité.
Ceci entraînerait des blessures graves, la mort ou des dommages matériels à ce
produit ou à d’autres biens.

If the unit is not operated as specified in this manual it may impair the protection
provided by the unit.
Si l'unité n'est pas utilisée comme spécifié dans ce manuel il peut diminuer la
protection fournie par l'unité.

Do not position the unit so that it is difficult to operate the main disconnect
device.
Ne placez pas l'appareil de sorte qu'il est difficile de faire fonctionner le dispositif
principal de déconnexion.

Do not lift or move this equipment without assistance.
Ne pas soulever ou déplacer cet équipement sans assistance.

19

Safety Precautions for this Product

Electrical outlets in the cabinet are restricted to 5 amps (100-115v) or 3 amps
(230v) maximum current.
Prises électriques dans l'armoire sont limitées à 5 (100-115v) o 3 (230v) courant
maximum ampères.

Do not use any detachable power cord that is not adequately rated for the unit.

Ne pas utliser un fil électrique amovible qui n’est pas du tension nominale de

l’appareil.

The biosafety cabinet should be certified by a certification technician before its
initial use. The cabinet should be recertified whenever it is relocated, serviced or
at least annually thereafter. Filter integrity and airflow performance should be
verified before using the cabinet.

Some internal components of the biosafety cabinet may become contaminated
during operation of the unit. Only experienced personnel competent in
decontamination procedures should decontaminate the cabinet before servicing
these components. If you have any questions regarding certification agencies, or

need assistance in locating one, contact Labconco’s Product Service Department

at 800-821-5525 or 816-333-8811.

DO NOT load more than 150 lbs. (68 kg) in the work area. Exceeding this limit
may damage the work surface and its supports. Excessive weight in the cabinet
may increase the risk of it overturning, or failure of hydraulic lift stands, resulting
in the cabinet and stand overturning. If your application requires loading more
than 150 lbs. (68 kg), contact Labconco’s Product Service Department at 800­821-5525 or 816-333-8811 for assistance.

Avoid the use of flammable gases or solvents in the biosafety cabinet. Care must
be taken to ensure against the concentration of flammable or explosive gases or
vapors. An open flame should NOT be used in the biosafety cabinet. Open

flames will disrupt airflow patterns, burn the HEPA filter and/or damage the filter’s

adhesive. Gases under high pressure should not be used in the biosafety
cabinet, as they may disrupt its airflow patterns.

HEPA filters only remove particulate matter. Operations generating volatile toxic
chemicals or radionuclides must be evaluated carefully.

20

The media of HEPA filters is fragile and should not be touched. Avoid puncturing
either HEPA filter during installation or normal operation. If you suspect that a
HEPA filter has been damaged, DO NOT use the cabinet; contact a local
certification agency or Labconco at 800-821-5525 or 816-333-8811 for re­certification information.

The HEPA filters in the biosafety cabinet will gradually accumulate airborne
particulate matter from the room and from work performed in the cabinet. The
rate of accumulation will depend upon the cleanliness of the room air, operating
time and the nature of work being done in the cabinet. The Filter Gauge
accurately displays the amount of filter life remaining.

Proper operation of the cabinet depends largely upon its location and the
operator’s work habits.

Avoid direct exposure of plastic or coated materials to ultraviolet (UV) radiation.
Never bypass the UV safety interlock that only allows the UV light to work when
the sash is closed. When surface disinfecting the biosafety cabinet:

o Avoid splashing the disinfecting solution on skin or clothing.
o Ensure adequate ventilation.
o Carefully follow the disinfectant’s safety instructions.
o Always dispose of disinfecting solutions in accordance with local and national

laws.

o DO NOT allow disinfectants with high concentrations of free chlorine to

contact the stainless steel components of the biosafety cabinet for a long
period of time. Free chlorine will corrode stainless steel after extended
contact.

Biosafety cabinets should be decontaminated for any of the following reasons:

o Before maintenance work requiring entry into contaminated areas.
o Before HEPA filter changes.
o Before performing certification tests requiring entry into contaminated areas.
o Before relocating the cabinet.
o Before changing research programs.
o After the gross spill of biohazardous material or toxic chemicals.

21

3: Catalog Number Configurator

Logic+ and Axiom biosafety cabinets and Puricare procedure stations utilize catalog
numbers (also referred to as model numbers) where each digit of the catalog number
provides key information about the model. Use the configurator below to identify
specifics about your product.

30248xxx1

Product Group

3=Laminar Flow Products

Product Family

0= Logic+
1= PuriCare
2-4= Cell Logic+

Cabinet Type

2= Type A2
3= Type B2
4= Type C1

Cabinet Width

3= 3-foot Wide

4= 4-foot Wide

5= 5-foot Wide

6= 6-foot Wide

Sash Height

1= 10” sash opening
2= 12” sash opening
8= 8” sash opening

9= 9” sash opening

22

4: Serial Tag

If you need to contact Labconco regarding this product, it is required you provide the
serial number. The serial number provides all product details, including the revision
level under which the product was constructed. It is the single, most important piece of
information when assistance is requested.

Serial Tag Location

See Figure 4-1 for the location of the primary and secondary serial tags.

Dress Panel

Primary
Serial Tag

Secondary
Serial Tag

Data Plate

Dress Panel

Screw

Dress Panel
Screw

Factory
Test
Report

Wiring
Diagram

Behind
Dress
Panel

Figure 4-1

23

Serial Tag Information

The following information is located on the Serial Tags:

Primary Serial Tag

 Serial Number
 Catalog (Model) Number
 Revision
 Electrical Requirements

o Voltage
o Frequency
o Amperage
o Phase

Secondary Serial Tag

 Serial Number
 Catalog (Model) Number

Figure 4-2

Figure 4-3

24

5: Factory Test Report

The factory test report is a valuable reference for the product’s airflows and blower

setting(s), as well as information about the HEPA filters, and accessories that may be
factory installed.

Test Report Location

The test report is located on the blower/plenum cover, which is behind the front dress
panel. See Figure 5-1. The Dress Panel Screws must be removed and the Dress Panel
lifted off to reach the test report.

Dress Panel

Primary
Serial Tag

Secondary
Serial Tag

Data Plate

Dress Panel

Screw

Dress Panel
Screw

Factory
Test
Report

Wiring
Diagram

Behind
Dress
Panel

Figure 5-1

25

Test Report Information

See Figure 5-2 for a typical test report. Review this carefully to identify where on the
report specific information is located. Important information is highlighted below.

Note: The test report shown is an A2-12inch model with two internal blowers. A2-12inch and C1 model
reports list two blower speeds (supply and exhaust blower). All other models report one blower speed.

Figure 5-2

Catalog
Number

Serial

Number

Blower

Speed(s)

Inflow

(Avg.

Velocity)

Downflow

(Avg. Velocity)

26

6: Additional Product Information

All NSF-Listed biosafety cabinets are required by NSF49 regulations to display a Data
Plate on the front of the product in a readily visible location.

Data Plate Location

See Figure 6-1 for the Data Plate location.

Dress Panel

Primary
Serial Tag

Secondary
Serial Tag

Data Plate

Dress Panel

Screw

Dress Panel
Screw

Factory
Test
Report

Wiring
Diagram

Behind
Dress
Panel

Figure 6-1

27

Data Plate Information

The Data Plate provides the following important information for certifier use during on­site certifications:

 Nominal Airflows (Inflow and Downflow)
 Downflow Grid Test Point Locations
 Introduction Location for Test Aerosol
 Secondary Inflow Methodology
 NSF49 standard revision under which the biosafety cabinet is listed

Figure 6-2 shows a Type A2, 4-feet width Data Plate as an example to identify where
the required information is located on the Data Plate. All Labconco Data Plates are in
the same format.

Figure 6-2

28

7: Exhaust System Connections

Not all models of biosafety cabinets are connected to a remote or building exhaust
system. Type A2 models may recirculate their exhaust back to the laboratory, or
remove their exhaust air from the lab via a VentusTM Canopy (also referred to as a
Thimble Connection) connected to a remote (building) exhaust system. Type B2
models must always be connected to an exhaust system. Type C1 models may operate
in A-mode where the exhaust is recirculated to the laboratory, or B-mode when
connected to a remote exhaust system.

This section provides recommended best practices when connecting a biosafety cabinet
to a remote exhaust system to avoid operational problems with the cabinet. In addition,
the specific exhaust volumes and vacuum pressures required to properly operate each
cabinet model are listed by cabinet width and sash operating height.

If a biosafety cabinet is being altered from recirculating operation to ducted (exhausted)
operation in the field, it must be recertified by a qualified certifier before being used.

Exhaust Connection Best Practices

Note: Only connect the biosafety cabinet to a suitable exhaust system that is dedicated
to the biosafety cabinet, or dedicated to exhausting laboratory ventilation equipment.

Do NOT connect the biosafety cabinet to the building’s general HVAC system for room

exhaust.

Figure 7-1

Examine the location to ensure that it

accommodates the cabinet’s exhaust duct. The
area directly above the cabinet’s exhaust port

should be clear of structural elements, water and
utility lines, or other fixed obstructions. There
should be enough clearance to accommodate a
10-inch diameter duct. See Figure 7-1.

29

Avoid cabinet locations that require an elbow

directly above the cabinet’s exhaust connection

or an excessive number of elbows in the exhaust
system. There should be a straight length 10
duct diameters long between the cabinet
connection and any elbow, and between
subsequent elbows. See Figure 7-2.

Figure 7-3

The Inlet Relief Valve located on the top of the
cabinet is designed to draw a maximum of 100
CFM (170 m3/hr).

Attempting to draw additional room air through
the valve (room air exhaust), can result in
unstable cabinet operation. See Figure 7-3.

Figure 7-4

If additional room exhaust needs to be drawn
through the exhaust system, install an additional
duct and balancing damper downstream of the

cabinet’s damper. This will allow for proper

balancing of the system. See Figure 7-4.

Figure 7-2

This information applies to Canopy-Exhausted Type A2 and C1 (in B-Mode) only

30

Air-Tight Damper

On canopy-exhausted Type A2 and C1 (operating in B-mode) models, Labconco highly
recommends installing an air-tight damper above each biosafety cabinet when
connected to a building exhaust system. The air-tight damper allows for fine adjustment
of the exhaust air volume provided, in order to correctly set the necessary exhaust
airflow for each biosafety cabinet. It also allows the biosafety cabinet to be sealed off
from the building exhaust system, should it become necessary to do so.

Note: Type B2 models must always be connected to a remote exhaust system. Type B2
models have an air-tight damper built into the biosafety cabinet’s exhaust housing.

AIR-TIGHT DAMPER

DUCT COLLAR

BUILDING EXHAUST DUCT
(NOT INCLUDED)

AIRFLOW

ADJUSTMENT

HANDLE

BIOSAFETY CABINET
DUCT STUB

Figure 7-5

Visual appearance of biosafety cabinet and exhaust connection may vary by model.

31

Exhaust System Requirements

The exhaust system must be capable of moving the following volumes of exhaust air at
the negative pressures listed. The Airflow Volumes are the values recorded via direct
measurement using a flow hood at the front opening of the cabinet. The Concurrent
Balance Values are measured in the exhaust duct via traverse methodology, and will
always be higher due to differences in volume measurement methodologies.

For Type A2 models with Ventus Canopy Connections, see Table 7-1.
For Cell Logic+ Type A2 models with Ventus Canopy Connections, see Table 7-2
For Type B2 models, see Table 7-3.
For Type C1 models operating in B-mode, see Table 7-4.

Type A2 Models

1: Unlike Type B biosafety cabinets, the recommended vacuum will remain constant
throughout the life of the exhaust HEPA filter. Duct vacuums below 0.05 inches H2O
(12 Pa) or above 0.5 inches H2O (125 Pa) may result in erratic operation and throw an
alarm condition from the product.

2: WC = Inches of Water Column, typically expressed in units of inches H2O.

Logic+ Type A2 Model

Airflow Volume

Concurrent

Balance Value

Recommended

Duct Vacuum1

ft3/min

m3/hr

ft3/min

m3/hr

WC2

Pa

3-foot, 8" Sash

263

447

289

491

0.15

38

3-foot, 10" Sash

316

537

348

591

0.15

38

4-foot, 8" Sash

333

566

366

622

0.15

38

4-foot, 10" Sash

404

687

444

754

0.15

38

4-foot, 12" Sash

474

805

521

885

0.15

38

5-foot, 8" Sash

403

685

443

753

0.15

38

5-foot, 10" Sash

491

834

540

917

0.15

38

5-foot, 12" Sash

580

985

638

1084

0.15

38

6-foot, 8" Sash

473

804

520

883

0.15

38

6-foot, 10" Sash

579

984

637

1082

0.15

38

6-foot, 12" Sash

686

1166

755

1283

0.15

38

Table 7-1

32

Cell Logic+ Type A2 Models

1: Unlike Type B biosafety cabinets, the recommended vacuum will remain constant
throughout the life of the exhaust HEPA filter. Duct vacuums below 0.05 inches H2O
(12 Pa) or above 0.5 inches H2O (125 Pa) may result in erratic operation and throw an
alarm condition from the product.

2: WC = Inches of Water Column, typically expressed in units of inches H2O.

Type B2 Models

1: WC = Inches of Water Column, typically expressed in units of inches H2O.

Logic+ Type B2 Model

Airflow Volume

Concurrent

Balance Value

Recommended

Duct Vacuum

ft3/min

m3/hr

ft3/min

m3/hr

WC1

Pa

4-foot, 8" Sash

723

1228

852

1448

1.8

450

6-foot, 8" Sash

1083

1840

1265

2149

2.2

550

Table 7-3

Logic+ Type A2 Model

Airflow Volume

Concurrent

Balance Value

Recommended

Duct Vacuum1

ft3/min

m3/hr

ft3/min

m3/hr

WC2

Pa

3-foot, 9" Sash

290

492

319

541

0.15

38

4-foot, 9" Sash

369

627

405

688

0.15

38

5-foot, 9" Sash

447

760

492

835

0.15

38

6-foot, 9" Sash

526

894

579

983

0.15

38

Table 7-2

33

Type C1 Models

1: Unlike Type B biosafety cabinets, the recommended vacuum will remain constant
throughout the life of the exhaust HEPA filter. Duct vacuums below 0.05 inches H2O
(12 Pa) or above 0.5 inches H2O (125 Pa) may result in erratic operation and throw an
alarm condition from the product.

2: WC = Inches of Water Column, typically expressed in units of inches H2O.

Axiom Type C1 Model

Airflow Volume

Concurrent

Balance Value

Recommended

Duct Vacuum1

ft3/min

m3/hr

ft3/min

m3/hr

WC2

Pa

4-foot, 8" Sash

323

549

387

658

0.30

75

4-foot, 10" Sash

400

680

480

816

0.30

75

6-foot, 8" Sash

463

787

556

945

0.30

75

6-foot, 10" Sash

570

968

684

1162

0.30

75

Table 7-4

34

8: Configuration

This section provides instructions to access and understand the current configuration of
the biosafety cabinet, and make changes to the configuration.

Keypad button presses are shown as [BLUE WITH BRACKETS]. Menu screen
selections are shown as green italics.

Current Configuration

It is important to understand the current configuration of the biosafety cabinet for many
reasons, some of which include:

 Verify model and type
 Verify accessories installed
 Troubleshooting

The Info Screen provides information on the current configuration of the biosafety
cabinets, and details the current blower speed(s). The Info Screen is not password
protected. To access this screen, follow these steps:

1. From the Main Menu, press [MENU], using [UP] or [DOWN] select the Tools
icon. Press [OK/MUTE].

[MENU]

Tools

35

2. The Info icon should be highlighted, if not select Info, press [OK/MUTE].

*Option(s) not installed are shown with a strikethrough. To activate options (after field
installation), see Change Configuration later in Section 8. To change the blower
speed(s), see Section 9: Calibration and Certification.

Type A2 12

-inch

and C1

Info

AFS

UV LIGHT

CANOPY

C1 - A MODE

REV. D

10 INCH

[OK/MUTE]

58

CURRENT / AVG = 1217 / 1219

NEW FILTER / MAX = 1074 / 1800

REV. D

SUPPLY

51

CURRENT / AVG = 1103 / 1104

NEW FILTER / MAX = 994 / 1800

REV. D

EXHAUST

[UP] [DOWN]

[UP] [DOWN]

Type

Sash

Height

Option(s)
Installed *

Software

Version

Blower

PWM

Only shown on two

blower models

(A2 12-inch and C1)

Instantaneous Blower RPM /

Time dampened average Blower RPM

Blower RPM with new HEPA filters /

Maximum Blower RPM

This screen only shown

on two blower models

(A2 12-inch and C1)

36

Change Configuration

Changes to the biosafety cabinet configuration can be made in the field. This typically
occurs after an accessory is field installed, or the user requests a change to the sash
height (between 8 and 10 inches) on a Type C1 only.

Never change the following configuration parameters:

 Cabinet Type – if the cabinet type is changed, it can result in incorrect operation

of the biosafety cabinet, and the biosafety cabinet may not protect the product,
personnel, or environment.

 Sash Height – On Type A2 and B2 cabinets, never change the sash height.

The following instructions detail all screens in the Configuration menu, which is
password protected. The password is [LIGHT] [UV LIGHT] [TIMER] [TIMER]

[OK/MUTE].

1. From the Main Menu, press [MENU], using [UP] or [DOWN] select the Tools
icon. Press [OK/MUTE].

2. Use [UP] or [DOWN] to select the Password icon, press [OK/MUTE].

[MENU]

Tools

Password

[OK/MUTE]

WARNING !

RESTRICTED AREA

FOR TECHNICIAN

USE ONLY

37

3. From the Warning Screen, press [OK/MUTE] to acknowledge the warning that
the following screens are restricted.

4. On the Password Screen, enter the password: [LIGHT] [UV LIGHT] [TIMER]

[TIMER] [OK/MUTE].

5. Configuration should be highlighted, if not, select it, press [OK/MUTE]. The first
screen will display the biosafety cabinet type. Do NOT change this selection,
press [OK/MUTE].

Type

C1

CONFIGURATION

CALIBRATION

PASSWORD

* * * * *

Password

Entered

TYPE A2

TYPE B2

TYPE C1

[OK/MUTE]

RECIRCULATE (A MODE)

EXHAUST (B MODE)

AIRFLOW SENSOR

AIRFLOW SENSOR

[OK/MUTE]

This screen only shown

on Type C1 models

If changing between A-

Mode and B-Mode on a

Type C1, do so here

Type B2 models must have an

Airflow Sensor, so this screen is

not shown on a Type B2

38

6. On a Type A2 or C1 only, if field installing, or removing the Airflow Sensor,
change the selection accordingly, press [OK/MUTE].

7. On all biosafety cabinet types, if field installing, or removing the UV Light, change
the selection accordingly (screen shown above), press [OK/MUTE].

8. On Type A2 models only, if field installing, or removing the Canopy connection,
change the selection accordingly, press [OK/MUTE].

Note: When a Type C is selected previously, the Canopy Screen will not appear, but
the software will default the selection correctly based on whether A-mode (No Canopy)
or B-mode (Canopy) was previously selected.

9. The Sash Height selection will display next, and should NOT be changed, unless
the model is a C1, and the user has decided to switch between 8-inch and 10­inch sash operation. Press [OK/MUTE].

If the biosafety cabinet is a Type A2 or B2, this is the final screen selection. If a C1,
proceed to step #10.

AIRFLOW SENSOR

AIRFLOW SENSOR

[OK/MUTE]

UV LIGHT

UV LIGHT

CANOPY

CANOPY

SASH HEIGHT

8 INCH

[OK/MUTE]

39

10. If a Type C1 model, and B-Mode operation was selected previously, the Active

Protection Screen will appear. This allows the selection of the time for which the

Type C1 will continue to operate (while sounding an alarm) when a remote
(building) exhaust failure occurs. This allows the user time to secure critical work
and exit before the cabinet shuts off. Set in 10 second increments up to 5
minutes (300 seconds). Use [UP] or [DOWN] to select the Active Protection
time. Press [OK/MUTE].

C1

B MODE

ACTIVE PROTECTION

10 SECONDS

40

9: Calibration

This section provides instructions to access and understand the procedures to calibrate
the biosafety cabinet. Use this section in conjunction with Section 10: Certification when
performing initial or annual certification.

Never enter the password-protected area, or change settings of the biosafety cabinet if
you are not a trained and qualified certifier or technician. Changing parameters in the
password-protected area may impair the product’s performance and result in loss of
protection and/or harm or death to personnel in the laboratory.

Certifier Password

The certifier password is: [LIGHT] [UV LIGHT] [TIMER] [TIMER] [OK/MUTE].
Use this password for all normal calibration and certification activities.
If installing or replacing an Airflow Sensor, the sensor will need a zero point calibration.

In order to access the zero-point calibration screen, a different password is required.
This password is: [LIGHT] [UV LIGHT] [TIMER] [OUTLET] [OK/MUTE].

41

Adjusting Blower Speed

During initial or annual certification of the biosafety cabinet, the blower speed may need
to be adjusted. Blower speed must only be adjusted by a trained, qualified certifier. To
adjust the blower speed, following these instructions.

1. From the Main Menu, press [MENU], using [UP] or [DOWN] select the Tools
icon. Press [OK/MUTE].

2. Use [UP] or [DOWN] to select the Password icon, press [OK/MUTE].

3. From the Warning Screen, press [OK/MUTE] to acknowledge the warning that
the following screens are restricted.

[MENU]

Tools

Password

[OK/MUTE]

WARNING !

RESTRICTED AREA

FOR TECHNICIAN

USE ONLY

42

4. On the Password Screen, enter the password: is [LIGHT] [UV LIGHT] [TIMER]

[TIMER] [OK/MUTE]. If also installing a new airflow sensor, see Certifier

Password earlier in this section to enter the correct password to allow for zero-

point calibration of the airflow sensor.

5. Use [UP] or [DOWN] to select Calibration. Press [OK/MUTE].

Note: The sash must be at operating height when pressing [OK/MUTE]. Access will
not be granted if the sash is closed.

Use [UP] or [DOWN] to adjust the blower speed. Each single press of [UP] or

[DOWN] increments the PWM signal sent to the blower by 0.5. It may take two

presses of the button to see the displayed PWM value change; however the
blower speed will adjust with each button press.

When the Instantaneous Blower rpm is within 15 rpm of the Average Blower rpm,
the blower speed has stabilized, and [OK/MUTE] can be pressed to move to the
next screen.

CONFIGURATION

CALIBRATION

PASSWORD

* * * * *

Password

Entered

58

CURRENT RPM = 1217

AVERAGE RPM = 1219

‘OK’ TO CONTINUE

SUPPLY

Blower

PWM

Only shown on two

blower models

(A2 12-inch and C1)

Instantaneous

Blower RPM

‘OK’ displayed

when blower

speed stabilizes

43

Adjusting the Internal Air Damper

On Type A2 8-inch through 10-inch sash heights and all Type B2 (single blower)
models, during the certification of the biosafety cabinet, it may be necessary to adjust
the split of air between downflow and exhaust. This is an important part of balancing
the biosafety cabinet and hitting the specified average downflow and inflow values
specified for each model and size cabinet.

Note: On Type A2 12-inch sash height and Type C1 (dual blower) models, there is no
internal air damper. One blower is dedicated to the supply (downflow) air, and one to
exhaust (inflow) air. On these models, the blower speed of each will be set
electronically. See the previous section Adjusting Blower Speed, and note the text

“Supply” displayed on Step #5. After setting the supply blower speed, the screens will

advance to the exhaust blower speed adjustment.
To adjust the internal damper, follow these instructions:

1. Remove the front Dress Panel (reference Figure 5-1 and 9-1) by removing the
two (2) Dress Panel Screws, pulling the bottom of the Dress Panel away from the
cabinet, and the lifting up on the Dress Panel.

CAUTION: The Dress Panel is large and heavy, especially on models 5-feet in width
and larger. Use two (2) persons to safely lift and remove the Dress Panel.

Figure 9-1

Dress Panel

Screws

44

2. Locate the exhaust damper adjustment bolt (reference Figure 9-2). The
adjustment bolt is in the center of a flexible, orange cup seal. Use a 7/16-inch nut
driver or socket and ratchet to turn the bolt. The bolt has 16 turns from fully open
to fully closed. The adjustment decal shows the number of turns from fully closed
that balanced the biosafety cabinet correctly at the factory. The adjustment bolt
and adjustment decal are shown in Figure 9-2.

Figure 9-2

Turning Counterclockwise:

 Decreases Inflow (Exhaust)
 Increases Downflow

Adjustment
Decal

Adjustment
Bolt

Turning Clockwise:

 Increases Inflow (Exhaust)
 Decreases Downflow

45

Setting the Filter Life Gauge

During initial or annual certification of the biosafety cabinet, and after the HEPA filters
are changed, the Filter Life Gauge may need to be reset. There are three options to
choose from when resetting the Filter Life Gauge. Below are the three options, and an
explanation on which to select.

1. New Filter – Select this option when the HEPA filters are new. This occurs
during initial certification of a new biosafety cabinet, or after new HEPA filters are
installed and the biosafety cabinet’s Inflow and Downflow have been verified by a
trained and qualified certifier.

2. Same Filter – Select this option when passing through this screen in the
Calibration submenu to reach the sensor calibrations, or when the blower
speed(s) have been adjusted slightly (but the HEPA filters have not been
replaced) during an annual certification.

3. Set Filter Life – This option is seldom selected. It may be used to set the Filter
Life Gauge to a specific percentage, for example, after the display circuit board is
replaced, and the settings from the previous display board need to be
programmed into the new display board.

To access the Filter Life Gauge screen(s), follow the steps in Adjusting the Blower
Speed previously shown in this section, until on the Blower Speed screen. Then follow
these instructions:

Type A2 (8-inch through 10-inch sash) and Type B2

1. On the Blower Speed screen, press [OK/MUTE].

2. Use [UP] or [DOWN] to select one of the three Filter Life options displayed,
press [OK/MUTE].

58

CURRENT RPM = 1217

AVERAGE RPM = 1219

‘OK’ TO CONTINUE

‘OK’ displayed when blower speed stabilizes

NEW FILTER

SAME FILTER

SET FILTER LIFE

[OK/MUTE]

46

Type A2 (12-inch sash) and Type C1

1. On the Supply Blower Speed screen, press [OK/MUTE].

2. Use [UP] or [DOWN] to select one of the three Filter Life options displayed,
press [OK/MUTE].

3. On the Exhaust Blower Speed screen, press [OK/MUTE].

4. Use [UP] or [DOWN] to select one of the three Filter Life options displayed,
press [OK/MUTE].

Note: On dual blower models, each filter has a separate Life Gauge for percentage of
filter remaining. The lower of the two Filter Life percentages is displayed on the Home
Screen.

58

CURRENT RPM = 1217

AVERAGE RPM = 1219

‘OK’ TO CONTINUE

‘OK’ displayed when blower speed stabilizes

NEW FILTER

SAME FILTER

SET FILTER LIFE

[OK/MUTE]

SUPPLY

SUPPLY

51

CURRENT RPM = 1193

AVERAGE RPM = 1075

‘OK’ TO CONTINUE

‘OK’ displayed when blower speed stabilizes

NEW FILTER

SAME FILTER

SET FILTER LIFE

[OK/MUTE]

EXHAUST

EXHAUST

47

Airflow Sensor Calibration

All Type B2 biosafety cabinets have an airflow sensor to sense the exhaust air pulled
from the cabinet by the remote (building) exhaust system. Type A2 and C1 biosafety
cabinets may have an airflow sensor as an optional accessory.

Unless installing or replacing the airflow sensor, the zero point should be correctly set
from the factory (see Zero Point Calibration later in this section). Once proper airflows
have been set by a trained and qualified certifier, the nominal set point should be
calibrated after setting the Filter Life Gauge in the Calibration menu. For a Type B2
biosafety cabinet, both the nominal and alarm set point will be calibrated.

Type A2 and Type C1

Note: If installing an airflow sensor on an A2 or C1 biosafety cabinet in the field, which
did not previously have this accessory installed, first enable the Airflow Sensor in

Configuration, as described in Change Configuration in Section 8: Configuration.

To access the Airflow Sensor Calibration screen(s), follow the steps in Adjusting the

Blower Speed and Setting the Filter Life Gauge previously shown in this section, until on

the Filter Life Selection screen. Then follow these instructions:

1. On the Filter Life Selection screen, press [OK/MUTE].

2. Using the average inflow velocity measured during airflow certification, use [UP]
or [DOWN] to adjust the Nominal Inflow Value to match this measured velocity.
The Nominal Inflow Value will flash, and it will be displayed in feet per minute
(FPM) or meters per second (M/S) based on the desired units selected in the

Settings submenu. Once set, press [OK/MUTE].

NEW FILTER

SAME FILTER

SET FILTER LIFE

[OK/MUTE]

INFLOW = 105 FPM

INFLOW

CALIBRATION

‘OK’ TO CONTINUE

Nominal

Inflow
Value

48

3. Using the average downflow velocity measured during airflow certification, use

[UP] or [DOWN] to adjust the Nominal Downflow Value to match this measured

velocity. The Nominal Downflow Value will flash, and it will be displayed in feet
per minute (FPM) or meters per second (M/S) based on the desired units
selected in the Settings submenu. Once set, press [OK/MUTE].

Note: Type C1 models with an airflow sensor will only display Inflow on the Home Screen.

Type B2

To access the Airflow Sensor Calibration screen(s), follow the steps in Adjusting the

Blower Speed and Setting the Filter Life Gauge previously shown in this section, until on

the Filter Life Selection screen. Then follow these instructions:

1. On the Filter Life Selection screen, press [OK/MUTE].

2. Using the average inflow velocity measured during airflow certification, use [UP]
or [DOWN] to adjust the Nominal Inflow Value to match this measured velocity.
The Nominal Inflow Value will flash, and it will be displayed in feet per minute
(FPM) or meters per second (M/S) based on the desired units selected in the

Settings submenu. Once set, press [OK/MUTE].

DOWNFLOW = 56 FPM

DOWNFLOW

CALIBRATION

‘OK’ TO CONTINUE

NEW FILTER

SAME FILTER

SET FILTER LIFE

[OK/MUTE]

INFLOW = 105 FPM

INFLOW

CALIBRATION

‘OK’ TO CONTINUE

Nominal

Inflow
Value

[OK/MUTE]

A2

C1

Returns to Main Menu

Proceeds to Vacuum Start

Switch Calibration

Nominal

Downflow

Value

49

3. The nominal set point for Inflow has been set in the previous step. Now the
alarm set point must be established. The screen below is displayed. While on
this screen, reduce the remote or building exhaust system until the TOTAL
volume of air drawn by the remote exhaust system is reduced to 90% of nominal.
For a B2 biosafety cabinet, the remote exhaust system pulls both the downflow
air volume AND inflow air volume from the cabinet and exhausts it.

4. Once the total exhaust airflow has been reduced to 90% of nominal, look at the

Current Sensor Value. It should be at least 4 or 5 points less than the Nominal
Sensor Value. These values are not velocity or flow values, they are the raw

digital output values from the airflow sensor. To avoid nuisance alarms in
operation, these two values should be at least 4 or 5 points apart. Press

[OK/MUTE].

5. Restore the remote exhaust system to nominal airflow. Press [OK/MUTE].

REDUCE EXHAUST FLOW

TO 90%, PRESS OK

AIR SENSOR: 219

ZERO / NOMINAL: 95 / 220

Logic+ Type B2 Model

Inflow @ 90%

Alarm Set Point

ft3/min

m3/hr

4-foot, 8" Sash

216

367

6-foot, 8" Sash

322

547

Note: To set alarm point airflow, if unable to
measure airflow in the exhaust duct above the
biosafety cabinet, slowly close the Air-Tight
Damper until the Inflow into the cabinet equals
the values in Table 9-1.

Table 9-1

Nominal

Sensor

Value

Current

Sensor

Value

RESTORE EXHAUST FLOW

TO 100%, PRESS OK

AIR SENSOR: 219

ZERO / NOMINAL: 95 / 220

50

Zero Point Calibration

If a new airflow sensor is being calibrated (field installation or replacement), a zero point
calibration will be performed. To gain access to the Zero Point Calibration screens, a
different password must be entered on the Password Screen. This password is:

[LIGHT] [UV LIGHT] [TIMER] [OUTLET] [OK/MUTE]

Upon entering this password successfully, select Calibration on the first screen shown.
Press [OK/MUTE]. Follow these instructions:

1. Before entering the Blower Speed screen(s), the Zero Point Warning screen is

shown (below). The biosafety cabinet’s internal blower(s) will automatically shut

off. If the biosafety cabinet is connected to a remote exhaust system, stop all
exhaust airflow before proceeding. Once all airflow is removed, press

[OK/MUTE].

2. The biosafety cabinet will display Wait for approximately 15 seconds while it
samples the airflow sensor readings. When complete, the screen below is
shown. At this time, if the biosafety cabinet is connected to a remote exhaust
system, restart the remote exhaust now. Press [OK/MUTE].

3. Zero point calibration is now complete. The follow screens will proceed through
setting the blower speed(s) as would normally be done if the standard password
had been entered.

AFS ZERO POINT

INTERNAL BLOWER WILL

SHUT OFF NOW. IF

CONNECTED TO EXHAUST

SYSTEM, STOP ALL

EXHAUST AIR FOR PROPER

ZERO POINT CALIBRATION

‘OK’ ONCE EXHAUST AIR REMOVED

[OK/MUTE]

AFS ZERO POINT

WAIT

IF CABINET CONNECTED TO

EXHAUST SYSTEM,

RESTART EXHAUST

SYSTEM NOW

‘OK’ WHEN EXHAUST AIR ON

AFS ZERO POINT

51

Vacuum Start Switch Calibration (Type C1 only)

A Type C1 biosafety cabinet operating in B-mode must sense sufficient remote exhaust
volume before starting the internal blowers. This is accomplished with the Vacuum
Start Switch (VSS) located in the exhaust housing of the biosafety cabinet. After setting
the Filter Life Gauge, or calibrating the Airflow Sensor (if present), a Type C1 biosafety
cabinet will ask if the VSS needs to be calibrated.

The VSS is factory calibrated, and should not need recalibration. If the biosafety
cabinet fails to start its internal blowers on demand, and an Exhaust Failure Alarm is
displayed after the Exhaust Airflow Check fails, the VSS may need to be recalibrated if it
has been determined the remote exhaust system is pulling the correct airflow volume.

To reach the VSS calibration screen, access the Calibration submenu as described in

Adjusting the Blower Speed earlier in this section. Pass through the Blower Speed,

Filter Life, and (if present) Airflow Sensor Calibration screens using [OK/MUTE].

If not recalibrating the VSS, select No on the screen below and press [OK/MUTE]. To
recalibrate the VSS, select Yes on the screen below and press [OK/MUTE]. Follow
these instructions:

1. Identify the VSS and Inlet Relief Valve atop the cabinet, see Figure 9-3. The Inlet
Relief Valve is a clear flap; the VSS is a black cube with clear hose protruding.

Figure 9-3

VSS

INLET RELIEF

VALVE

AIRFLOW

ADJUSTMENT

HANDLE

DUCT COLLAR ON

BIOSAFETY CABINET

AIR-TIGHT DAMPER

DUCT COLLAR ON

AIR-TIGHT DAMPER

EXHAUST DUCT

(NOT SUPPLIED)

52

1. After selecting Yes on the screen to the left below and pressing [OK/MUTE],
watch the Inlet Relief Valve and slowly reduce the remote exhaust system flow
until the Inlet Relief Valve begins to flutter, but is not completely closed. Hold the
remote exhaust system flow at this point. The state of the Inlet Relief Valve can
be seen on the display screen. The Inlet Relief Valve should still be Open. In
this state, press [OK/MUTE].

2. When the screen below is shown, the biosafety cabinet’s internal blowers will
shut off. Using a small, flat blade screwdriver, if the VSS status shown is Open,
adjust the VSS set screw slowly clockwise until the VSS status changes to

Closed. See Figure 9-4 for reference. Then slowly turn the set screw

counterclockwise until the status changes to Open. Because the screen is not
readily visible when adjusting the set screw, the biosafety cabinet will play an
audible alarm tone whenever the VSS status is Closed. Stop turning the set
screw counterclockwise when this tone stops. The VSS Calibration is complete.

3. Restore the remote exhaust system to nominal airflow. Press [OK/MUTE].

YES NO

CALIBRATE VACUUM

START SWITCH?

INLET VALVE: OPEN

REDUCE EXHAUST UNTIL

INLET VALVE ALMOST CLOSED

‘OK’ TO CONTINUE

[OK/MUTE]

SWITCH: OPEN

ADJUST VSS SCREW SLOWLY

UNTIL SWITCH OPENS

‘OK’ TO CONTINUE

Figure 9-4

SET SCREW

INLET RELIEF

VALVE

VSS

53

10: Certification

This section provides instructions to certify the biosafety cabinet. Use this section in
conjunction with Section 9: Calibration when performing initial or annual certification.

Never enter the password-protected area, or change settings of the biosafety cabinet if
you are not a trained and qualified certifier or technician. Changing parameters in the
password-protected area may impair the product’s performance and result in loss of
protection and/or harm or death to personnel in the laboratory.

Certifier Password

The certifier password is: [LIGHT] [UV LIGHT] [TIMER] [TIMER] [OK/MUTE].
Use this password for all normal calibration and certification activities.
If installing or replacing an Airflow Sensor, the sensor will need a zero point calibration.

In order to access the zero-point calibration screen, a different password is required.
This password is: [LIGHT] [UV LIGHT] [TIMER] [OUTLET] [OK/MUTE].

Type A2 HEPA Filter Leak Test Preparation

All biosafety cabinet models were tested to the HEPA Filter Leak Test as described in
the current NSF/ANSI Standard 49. This section describes the methods, specifications,
and Pass/Fail criteria to challenge each HEPA filter in the biosafety cabinet.

You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.

1. Remove the work surface by lifting the handles on either end. Pull the work
surface straight out of the biosafety cabinet.

2. Turn the blower(s) of the biosafety cabinet on.

3. Place the aerosol generator in the left, rear corner of the biosafety cabinet’s drip
pan, see Figure 10-2. Ensure that the generator is level, and the oil level is
within 1/8 inch (3 mm) of the level line.

54

4. If the model has a pre-filter installed on the towel catch, remove it for testing.

5. If the aerosol generator requires pressurized air, connect the air line. Turn on the
appropriate number of Laskin nozzles for the model under test based on Table
10-1 to ensure they are each working properly.

6. Verify the generator’s air pressure is 23 psi.

7. Turn on the photometer and allow it to operate for a minimum of 5 minutes.
Leave the valve in the “CLEAR” setting.

Theoretical Upstream Concentration

If the biosafety cabinet has been used with biohazards, toxic chemicals, or
radioisotopes, the Upstream Sample Tube cannot be used (Figure 10-1). Testing at
Labconco has shown the actual concentration varies from the theoretical value. Table
10-1 shows the actual vs. calculated concentrations for Type A2 models. Establish the
100% and 0% concentration levels for the photometer using the actual values provided
in Table 10-1 for the model being tested.

Upstream Concentration Sampling

If the biosafety cabinet has NOT been used with biohazards, toxic chemicals, or
radioisotopes, the Upstream Sample Tube can be used (Figure 10-1).

You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.

1. Locate the Upstream Sample Tube on the right side, protruding from the rear
baffle. See Figure 10-1. On some models this tube is clear.

2. Pull the Upstream Sample Tube forward. Do not remove the cap yet.

3. When prompted by the photometer, turn on the appropriate number of Laskin
nozzles on the aerosol generator, uncap the Upstream Sampling Tube and
sample the upstream concentration to establish the 100% concentration level.

4. Establish the 0% concentration level according to your photometer’s instructions.

Figure 10-1

Figure 10-2

55

Type A2

Cabinet Width

Sash

Height

(inches)

Air

Displacement

(CFM)

Laskin

Nozzles

Required

Calculated

Concentration

(ug/L)1

Actual

Concentration

(ug/L)2

3-foot

8

550

1

25

14

9

570

1

24

12

10

600

1

23

12

4-foot

8

725

2

37

21

9

760

2

36

18

10

800

2

34

18

12

900

3

30

15

5-foot

8

900

2

30

17

9

950

2

28

14

10

1000

2

27

14

12

1130

3

24

13

6-foot

8

1100

2

25

17

9

1140

2

24

15

10

1200

2

23

15

12

1350

3

20

10

1. The calculated concentration was established using the formula:
Concentration (ug/l) = (# Laskin nozzles @ 23 PSIG x 13,500) / Volume of air displaced

2. Based on Mineral oil

Table 10-1

56

Type A2 Supply HEPA Filter Leak Test

During this test the supply HEPA filter will be challenged and scanned to check for
leaks. This section describes how to access and test the supply HEPA filter on a Type
A2 biosafety cabinet. The aerosol generator and photometer should be prepared at this
point as described in the preparation steps listed in the previous section.

1. Raise the glass sash as high as it will go.

2. Using a 7/16 inch nut driver or socket and ratchet, remove the two acorn nuts
along the front of the diffuser. See Figure 10-3. Note: Dress Panel and LED
lamps removed for clarity.

3. Lower the front edge of the diffuser until it clears the bottom of the sash, then pull
the diffuser straight out of the biosafety cabinet. See Figure 10-4.

4. The supply HEPA filter is now ready to be scanned. Set the photometer sampling
valve to “DOWNSTREAM”. Ensure proper vacuum at the sampling nozzle.

Figure 10-3

Acorn Nuts (2)

Note: The diffuser acorn nuts
are secured to the studs with a
removable thread locking
compound to prevent them from
vibrating loose during shipment.
There may be some resistance
the first time the nuts are
removed; this is normal. No
additional thread locking
compound need be applied to
these acorn nuts unless the unit
is being prepared for shipment.

Figure 10-4

Diffuser

HEPA Filter

57

5. Open the correct number of Laskin Nozzles on the aerosol generator (refer to
Table 10-1).

6. Scan the downstream side of the supply HEPA filter by passing the sampling
nozzle in slightly overlapping strokes over the entire surface of the filter. The
sampling nozzle must be no more than 1 inch from the surface of the filter media.
Scan at a traverse rate of not more than 2 inches per second.

7. Scan the entire periphery of the supply HEPA filter, including the gasket between
the filter frame and the biosafety cabinet structure.

Note: When scanning the front edge of the supply HEPA filter, photometer operation
may become erratic due to the aspiration of room air into the front of the work area.
This problem can be minimized or eliminated by placing the edge of a sheet of rigid
plastic or metal just outside the edge of the HEPA filter when scanning the front edge.
See Figure 10-5 as reference.

Acceptance Criteria

Aerosol penetration shall not exceed 0.01%.
Reinstall the diffuser, ensuring the two holes in the back of the diffuser frame engage

the posts on the rear wall of the cabinet. See Figure 10-6 for reference.

Figure 10-5

Figure 10-6

Diffuser Pins

engaged

correctly

58

Type A2 Exhaust HEPA Filter Leak Test

During this test the exhaust HEPA filter will be challenged and scanned to check for
leaks. This section describes how to access and test the exhaust HEPA filter on a Type
A2 biosafety cabinet. The aerosol generator and photometer should be prepared at this
point as described in the preparation steps listed in the previous section.

Before starting, examine the clearance between the top of the biosafety cabinet and the
ceiling or any overhead obstructions. If there is 18 inches (46 cm) or more of overhead
clearance, follow the procedure below to hinge the exhaust cover open. If there is not
enough clearance, remove the exhaust cover altogether.

1. Loosen the two (2) Phillips screws on the front sides of the exhaust filter cover,
see Figure 10-7. Tilt the cover back to access the downstream side of the filter.

The exhaust HEPA filter media is now exposed. Never touch the media, it is easily
damaged.

2. The exhaust HEPA filter is now ready to be scanned. Set the photometer

sampling valve to “DOWNSTREAM”. Ensure proper vacuum at the sampling

nozzle.

3. Open the correct number of Laskin Nozzles on the aerosol generator (refer to
Table 10-1).

4. Scan the downstream side of the exhaust HEPA filter by passing the sampling
nozzle in slightly overlapping strokes over the entire surface of the filter. The
sampling nozzle must be no more than 1 inch from the surface of the filter media.
Scan at a traverse rate of not more than 2 inches per second.

5. Scan the entire periphery of the exhaust HEPA filter, including the gasket
between the filter frame and the biosafety cabinet structure.

Figure 10-7

Loosen these screws

If removing the cover,

remove these screws

59

Note: When scanning the edges of the exhaust HEPA filter, photometer operation may

become erratic due to the aspiration of room air into the exhaust air stream. This
problem can be minimized or eliminated by placing the edge of a sheet of rigid plastic or
metal just outside the edge of the HEPA filter when scanning the filter edges. See
Figure 10-8 as reference.

Acceptance Criteria

Aerosol penetration shall not exceed 0.01%.

Replace and secure the exhaust cover and its screws.

Figure 10-8

60

Type B2 Supply HEPA Filter Leak Test Preparation

All biosafety cabinet models were tested to the HEPA Filter Leak Test as described in
the current NSF/ANSI Standard 49. This section describes the methods, specifications,
and Pass/Fail criteria to challenge the supply HEPA filter in the biosafety cabinet.

You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.

1. Remove the work surface by lifting the handles on either end. Pull the work
surface straight out of the biosafety cabinet.

2. Turn the blower of the biosafety cabinet on.

3. Check the oil level of the aerosol generator is within 1/8 inch (3 mm) of the level
line.

4. If the aerosol generator requires pressurized air, connect the air line.

5. Verify the generator’s air pressure is 23 psi.

6. Turn on the photometer and allow it to operate for a minimum of 5 minutes.
Leave the valve in the “CLEAR” setting.

Theoretical Upstream Concentration

If you do not wish to connect the Upstream Sampling Tube to the photometer to
establish the actual 100% concentration value, the theoretical value will need to be used
instead. Testing at Labconco has shown the actual concentration varies from the
theoretical value. Table 10-2 shows the actual vs. calculated concentrations for Type B2
models. Establish the 100% and 0% concentration levels for the photometer using the
actual values provided in Table 10-2 for the model being tested.

Upstream Concentration Sampling

You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.

1. Locate the Upstream Sample Tube on the right side, protruding from the rear
baffle. See Figure 10-9. On some models this tube is clear.

2. Pull the Upstream Sample Tube forward. Do not remove the cap yet.

3. Place the aerosol generator on top of the biosafety cabinet, see Figure 10-10.

4. Remove the inlet pre-filter (Figure 10-10).

5. When prompted by the photometer, turn on the appropriate number of Laskin
nozzles on the aerosol generator (see Table 10-2), uncap the Upstream
Sampling Tube and sample the upstream concentration to establish the 100%
concentration level.

6. Establish the 0% concentration level according to your photometer’s instructions.

61

Figure 10-9

Figure 10-10

Type B2

Cabinet Width

Sash

Height

(inches)

Supply Air

Displacement

(CFM)

Laskin

Nozzles

Required

Calculated

Concentration

(ug/L)1

Actual

Concentration

(ug/L)2

4-foot

8

445

1

30

15

6-foot

8

665

1

20

12

1. The calculated concentration was established using the formula:
Concentration (ug/l) = (# Laskin nozzles @ 23 PSIG x 13,500) / Volume of air displaced

2. Based on Mineral oil

Table 10-2

Pre-Filter

Thumbnuts (2)

Place aerosol

generator here

62

Type B2 Supply HEPA Filter Leak Test

During this test the supply HEPA filter will be challenged and scanned to check for
leaks. This section describes how to access and test the supply HEPA filter on a Type
B2 biosafety cabinet. The aerosol generator and photometer should be prepared at this
point as described in the preparation steps listed in the previous section.

1. Raise the glass sash as high as it will go.

2. Using a 7/16 inch nut driver or socket and ratchet, remove the two acorn nuts
along the front of the diffuser. See Figure 10-11. Note: Dress Panel and LED
lamps removed for clarity.

3. Lower the front edge of the diffuser until it clears the bottom of the sash, then pull
the diffuser straight out of the biosafety cabinet. See Figure 10-12.

4. The supply HEPA filter is now ready to be scanned. Set the photometer sampling
valve to “DOWNSTREAM”. Ensure proper vacuum at the sampling nozzle.

Figure 10-11

Acorn Nuts (2)

Note: The diffuser acorn nuts
are secured to the studs with a
removable thread locking
compound to prevent them from
vibrating loose during shipment.
There may be some resistance
the first time the nuts are
removed; this is normal. No
additional thread locking
compound need be applied to
these acorn nuts unless the unit
is being prepared for shipment.

Figure 10-12

Diffuser

HEPA Filter

63

5. Open the correct number of Laskin Nozzles on the aerosol generator (refer to
Table 10-2).

6. Scan the downstream side of the supply HEPA filter by passing the sampling
nozzle in slightly overlapping strokes over the entire surface of the filter. The
sampling nozzle must be no more than 1 inch from the surface of the filter media.
Scan at a traverse rate of not more than 2 inches per second.

7. Scan the entire periphery of the supply HEPA filter, including the gasket between
the filter frame and the biosafety cabinet structure.

Note: When scanning the front edge of the supply HEPA filter, photometer operation
may become erratic due to the aspiration of room air into the front of the work area.
This problem can be minimized or eliminated by placing the edge of a sheet of rigid
plastic or metal just outside the edge of the HEPA filter when scanning the front edge.
See Figure 10-13 as reference.

Acceptance Criteria

Aerosol penetration shall not exceed 0.01%.
Reinstall the inlet pre-filter and diffuser, ensuring the two holes in the back of the

diffuser frame engage the posts on the rear wall of the cabinet. See Figure 10-14 for
reference.

Figure 10-13

Figure 10-14

Diffuser Pins

engaged

correctly

64

Type B2 Exhaust HEPA Filter Leak Test Preparation

All biosafety cabinet models were tested to the HEPA Filter Leak Test as described in
the current NSF/ANSI Standard 49. This section describes the methods, specifications,
and Pass/Fail criteria to challenge the exhaust HEPA filter in the biosafety cabinet.

You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.

1. Remove the work surface by lifting the handles on either end. Pull the work
surface straight out of the biosafety cabinet.

2. Turn the blower of the biosafety cabinet on.

3. Place the aerosol generator in the left, rear corner of the biosafety cabinet’s drip
pan, see Figure 10-15. Ensure that the generator is level, and the oil level is
within 1/8 inch (3 mm) of the level line.

4. If the aerosol generator requires pressurized air, connect the air line.

5. Verify the generator’s air pressure is 23 psi.

6. Turn on the photometer and allow it to operate for a minimum of 5 minutes.
Leave the valve in the “CLEAR” setting.

Theoretical Upstream Concentration

Actual upstream sampling is not possible on a Type B2 exhaust filter, so the theoretical
concentration will be used. Testing at Labconco has shown the actual concentration
varies from the theoretical value. Table 10-3 shows the actual vs. calculated
concentrations for Type B2 models. Establish the 100% and 0% concentration levels for
the photometer using the theoretical values provided in Table 10-3 for the model being
tested.

Figure 10-15

65

Type B2 Exhaust HEPA Filter Leak Test

During this test the exhaust HEPA filter will be challenged and a downstream average
utilized to check for leaks. This section describes how to test the exhaust HEPA filter
on a Type B2 biosafety cabinet. The aerosol generator and photometer should be
prepared at this point as described in the preparation steps listed in the previous
section.

1. Locate a sampling port downstream of the exhaust HEPA filter. The sampling
port is not part of the biosafety cabinet. It must be facility supplied as part of the

facility’s exhaust system ducting. The sampling port should ideally be at least 10

duct diameters downstream of the exhaust HEPA filter, and the same distance
from any elbows in the duct work.

2. Connect the sampling port to the photometer.

3. Set the photometer sampling valve to “DOWNSTREAM”. Ensure proper vacuum
at the sampling tube.

4. Open the appropriate number of Laskin nozzles on the aerosol generator (see
Table 10-3).

5. Observe the average concentration of aerosol downstream.

Acceptance Criteria

Aerosol penetration shall not exceed 0.005%.
Reinstall the work surface.

Type B2

Cabinet Width

Sash

Height

(inches)

Exhaust Air

Displacement

(CFM)

Laskin

Nozzles

Required

Calculated

Concentration

(ug/L)1

Actual

Concentration

(ug/L)2

4-foot

8

723

2

38

21

6-foot

8

1083

2

25

17

1. The calculated concentration was established using the formula:
Concentration (ug/l) = (# Laskin nozzles @ 23 PSIG x 13,500) / Volume of air displaced

2. Based on Mineral oil

Table 10-3

66

Type C1 Supply HEPA Filter Leak Test Preparation

All biosafety cabinet models were tested to the HEPA Filter Leak Test as described in
the current NSF/ANSI Standard 49. This section describes the methods, specifications,
and Pass/Fail criteria to challenge the supply HEPA filter in the biosafety cabinet.

You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.

1. Remove the center work surface by lifting the handles on either end. Pull the
work surface straight out of the biosafety cabinet. Remove the two work surface
wings.

2. Turn the blower of the biosafety cabinet on.

3. Place the aerosol generator on the center drip tray exposed when the center
work surface was removed, see Figure 10-16.

4. Use an extension tube (see Figure 10-16) on the aerosol generator to ensure the
challenge aerosol is delivered to the left most part of the rear baffle. The
challenge must not be delivered to the center portion of the rear baffle (defined
by the center drip tray the generator is resting on).

5. Check the oil level of the aerosol generator is within 1/8 inch (3 mm) of the level
line.

6. If the aerosol generator requires pressurized air, connect the air line.

7. Verify the generator’s air pressure is 23 psi.

8. Turn on the photometer and allow it to operate for a minimum of 5 minutes.
Leave the valve in the “CLEAR” setting.

Theoretical Upstream Concentration

If you do not wish to connect the Upstream Sampling Tube to the photometer to
establish the actual 100% concentration value, the theoretical value will need to be used
instead. Testing at Labconco has shown the actual concentration varies from the
theoretical value. Table 10-4 shows the actual vs. calculated concentrations for Type C1
models. Establish the 100% and 0% concentration levels for the photometer using the
actual values provided in Table 10-4 for the model being tested.

Upstream Concentration Sampling

You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.

1. Locate the Upstream Sample Tube on the right side, protruding from the rear
baffle. See Figure 10-17. On some models this tube is clear.

2. Pull the Upstream Sample Tube forward. Do not remove the cap yet.

3. When prompted by the photometer, turn on the appropriate number of Laskin
nozzles on the aerosol generator (see Table 10-4), uncap the Upstream

67

Sampling Tube and sample the upstream concentration to establish the 100%
concentration level.

4. Establish the 0% concentration level according to your photometer’s instructions.

Figure 10-16

Figure 10-17

Type C1

Cabinet Width

Sash

Height

(inches)

Supply Air

Displacement

(CFM)

Laskin

Nozzles

Required

Calculated

Concentration

(ug/L)1

Actual

Concentration

(ug/L)2

4-foot

8

520

1

26

13

10

520

1

26

13

6-foot

8

665

1

20

12

10

665

1

20

12

1. The calculated concentration was established using the formula:
Concentration (ug/l) = (# Laskin nozzles @ 23 PSIG x 13,500) / Volume of air displaced

2. Based on Mineral oil

Table 10-4

Center Drip Tray

Extension Tube

68

Type C1 Supply HEPA Filter Leak Test

During this test the supply HEPA filter will be challenged and scanned to check for
leaks. This section describes how to access and test the supply HEPA filter on a Type
C1 biosafety cabinet. The aerosol generator and photometer should be prepared at this
point as described in the preparation steps listed in the previous section.

1. Raise the glass sash as high as it will go.

2. Using a 7/16 inch nut driver or socket and ratchet, remove the two acorn nuts
along the front of the diffuser. See Figure 10-18. Note: Dress Panel and LED
lamps removed for clarity.

3. Lower the front edge of the diffuser until it clears the bottom of the sash, then pull
the diffuser straight out of the biosafety cabinet. See Figure 10-19.

4. The supply HEPA filter is now ready to be scanned. Set the photometer sampling
valve to “DOWNSTREAM”. Ensure proper vacuum at the sampling nozzle.

Figure 10-18

Acorn Nuts (2)

Note: The diffuser acorn nuts
are secured to the studs with a
removable thread locking
compound to prevent them from
vibrating loose during shipment.
There may be some resistance
the first time the nuts are
removed; this is normal. No
additional thread locking
compound need be applied to
these acorn nuts unless the unit
is being prepared for shipment.

Figure 10-19

Diffuser

HEPA Filter

69

5. Open the correct number of Laskin Nozzles on the aerosol generator (refer to
Table 10-4).

6. Scan the downstream side of the supply HEPA filter by passing the sampling
nozzle in slightly overlapping strokes over the entire surface of the filter. The
sampling nozzle must be no more than 1 inch from the surface of the filter media.
Scan at a traverse rate of not more than 2 inches per second.

7. Scan the entire periphery of the supply HEPA filter, including the gasket between
the filter frame and the biosafety cabinet structure.

Note: When scanning the front edge of the supply HEPA filter, photometer operation
may become erratic due to the aspiration of room air into the front of the work area.
This problem can be minimized or eliminated by placing the edge of a sheet of rigid
plastic or metal just outside the edge of the HEPA filter when scanning the front edge.
See Figure 10-20 as reference.

Acceptance Criteria

Aerosol penetration shall not exceed 0.01%.
Reinstall the diffuser, ensuring the two holes in the back of the diffuser frame engage

the posts on the rear wall of the cabinet. See Figure 10-21 for reference.

Figure 10-20

Figure 10-21

Diffuser Pins

engaged

correctly

70

Type C1 Exhaust HEPA Filter Leak Test Preparation

All biosafety cabinet models were tested to the HEPA Filter Leak Test as described in
the current NSF/ANSI Standard 49. This section describes the methods, specifications,
and Pass/Fail criteria to challenge the exhaust HEPA filter in the biosafety cabinet.

You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.

1. Remove the center work surface by lifting the handles on either end. Pull the
work surface straight out of the biosafety cabinet. Remove the two work surface
wings.

2. Turn the blower of the biosafety cabinet on.

3. Place the aerosol generator on the center drip tray exposed when the center
work surface was removed, see Figure 10-22.

4. Use an extension tube (see Figure 10-22) on the aerosol generator to ensure the
challenge aerosol is delivered to the center part of the rear baffle (defined by the
center drip tray the generator is resting on). The challenge must not be delivered
to the far left or right portion of the rear baffle.

5. Check the oil level of the aerosol generator is within 1/8 inch (3 mm) of the level
line.

6. If the aerosol generator requires pressurized air, connect the air line.

7. Verify the generator’s air pressure is 23 psi.

8. Turn on the photometer and allow it to operate for a minimum of 5 minutes.
Leave the valve in the “CLEAR” setting.

Theoretical Upstream Concentration

Actual upstream sampling is not possible on a Type C1 exhaust filter, so the theoretical
concentration will be used. Testing at Labconco has shown the actual concentration
varies from the theoretical value. Table 10-5 shows the actual vs. calculated
concentrations for Type C1 models. Establish the 100% and 0% concentration levels for
the photometer using the theoretical values provided in Table 10-5 for the model being
tested.

71

Figure 10-22

Type C1

Cabinet Width

Sash

Height

(inches)

Exhaust Air

Displacement

(CFM)

Laskin

Nozzles

Required

Calculated

Concentration

(ug/L)1

Actual

Concentration

(ug/L)2

4-foot

8

283

1

48

28

10

354

1

38

22

6-foot

8

423

1

32

19

10

529

1

26

15

1. The calculated concentration was established using the formula:
Concentration (ug/l) = (# Laskin nozzles @ 23 PSIG x 13,500) / Volume of air displaced

2. Based on Mineral oil

Table 10-5

72

Type C1 Exhaust HEPA Filter Leak Test

During this test the exhaust HEPA filter will be challenged and a downstream average
utilized to check for leaks. This section describes how to test the exhaust HEPA filter
on a Type C1 biosafety cabinet. The aerosol generator and photometer should be
prepared at this point as described in the preparation steps listed in the previous
section.

1. If the biosafety cabinet is connected to an exhaust system, secure the Inlet Relief

Valve in an open position (to keep the cabinet’s blowers running during the test).

Then remove the perimeter screws that secure the front plate of the exhaust
housing. Set the front plate aside. See Figure 10-23 for reference.

2. Set the photometer sampling valve to “DOWNSTREAM”. Ensure proper vacuum
at the sampling tube.

3. Open the appropriate number of Laskin nozzles on the aerosol generator (see
Table 10-5).

4. Using a rigid tube (18 inch long x 3/8 inch OD) connected to the photometer
probe pickup (see Figure 10-24), scan the downstream side of the filter, resting
the tube in the ridges located on the top of the opening (see Figures 10-24 &10-

25). By resting the tubing in successive ridges, and alternatively pushing the
probe in on the first ridge, then shifting the tube to the next ridge, and pulling it
out, you can quickly scan the filter surface in overlapping strokes. By resting your
index finger under the tube during scanning, you can accurately control the probe
position and height.

Figure 10-23

Exhaust
Housing

Front Plate

Inlet Relief Valve

Figure 10-24

Figure 10-25

Ridge cutouts

Rigid extension tube

Sampling Nozzle

73

5. Observe the average concentration of aerosol downstream.

Acceptance Criteria

Aerosol penetration shall not exceed 0.01%.
Reinstall the exhaust housing front plate, unsecure temporary Inlet Relief Valve hold

open, reinstall the work surface pieces.

74

Downflow Velocity

All models of Labconco biosafety cabinets are classified as uniform downflow, as tested
per the current NSF Standard 49. This section describes how to prepare and test the
downflow velocity on all Types of biosafety cabinets.

Before setting up the downflow velocity test, if any accessories are installed inside the
biosafety cabinet (UV lamp, IV Bar, etc.), remove them for this test. Ensure sash is at
the correct operating height, and remote (building) exhaust is on (if exhaust connected).

On all biosafety cabinet types and models, the downflow test points are to be taken at a
height of 4 inches (10 cm) above the bottom edge of the glass sash.

The thermal anemometer probe should always point toward the nearest side wall
or the sash, whichever is closer to the probe. Therefore, on the two rear rows, the

probe should point to the left side wall when taking the left half of test points, and the
right side wall when taking the right half of test points. And, on the front row, the probe
should always point forward towards the glass sash. See Figures 10-26 and 10-27 for
reference.

The thermal anemometer probe stand shown in the above figures is available from
Labconco. The catalog number for the stand is 3858410.

Set the thermal anemometer’s time constant to 10 or 15 seconds for greater data
stability.

1. For the Type and sash height biosafety cabinet under test, set up the test grid
based on the dimensions provided in Table 10-6. Start the blower(s).

Figure 10-26

Figure 10-27

75

2. Take the downflow velocity reading at each test point location of the test grid.

3. Average all individual test point velocity readings.

4. If the average is not within the acceptance criteria range, adjust the biosafety
cabinet according to Adjusting the Blower Speed and/or Adjusting the Internal
Damper found in Section 9: Calibration.

Acceptance Criteria

Average downflow shall be as specified in Table 10-6 column “Downflow Average” +/-5
FPM (+/-0.03 m/s), and all individual test points within +/-16 FPM (+/-0.081 m/s) from
the average.

Type
A2 & B2
Cabinet

Width

Sash

Height

Downflow

Average1

Test Grid

Distance

in inches (cm)

inches

FPM (m/s)

Total Test

Points

Rows x

Columns

From Back

& Sides

Between

Rows

Between

Columns

3-foot

8, 9

55 (.28)

21

3 x 7

6.0 (15)

5.75 (14.6)

4.08 (10.4)

10

55 (.28)

21

3 x 7

6.0 (15)

5.55 (14.1)

4.08 (10.4)

4-foot

8, 9

55 (.28)

24

3 x 8

6.0 (15)

5.75 (14.6)

5.21 (13.2)

10

55 (.28)

24

3 x 8

6.0 (15)

5.55 (14.1)

5.21 (13.2)

12

60 (.30)

24

3 x 8

6.0 (15)

5.44 (13.8)

5.21 (13.2)

5-foot

8, 9

55 (.28)

30

3 x 10

6.0 (15)

5.75 (14.6)

5.39 (13.7)

10

55 (.28)

30

3 x 10

6.0 (15)

5.55 (14.1)

5.39 (13.7)

12

60 (.30)

30

3 x 10

6.0 (15)

5.44 (13.8)

5.39 (13.7)

6-foot

8, 9

55 (.28)

36

3 x 12

6.0 (15)

5.75 (14.6)

5.50 (14.0)

10

55 (.28)

36

3 x 12

6.0 (15)

5.55 (14.1)

5.50 (14.0)

12

60 (.30)

36

3 x 12

6.0 (15)

5.44 (13.8)

5.50 (14.0)

Type C1

Cabinet

Width

Sash

Height

Downflow

Average1

Test Grid

Distance

in inches (cm)

inches

FPM (m/s)

Total Test

Points

Rows x

Columns

From Back

& Sides

Between

Rows

Between

Columns

4-foot

8

65 (.33)

24

3 x 8

6.0 (15)

5.75 (14.6)

5.21 (13.2)

10

65 (.33)

24

3 x 8

6.0 (15)

5.55 (14.1)

5.21 (13.2)

6-foot

8

55 (.28)

36

3 x 12

6.0 (15)

5.75 (14.6)

5.50 (14.0)

10

55 (.28)

36

3 x 12

6.0 (15)

5.55 (14.1)

5.50 (14.0)

1. Tolerance for Average Downflow Velocity = +/- 5 FPM (+/- 0.03 m/s)

Table 10-6

76

Inflow Velocity

All models of Labconco biosafety cabinets are tested per the current NSF/ANSI
Standard 49. This section describes how to prepare and test the inflow velocity on all
Types of biosafety cabinets.

The front Dress Panel must be installed on the biosafety cabinet to obtain accurate
inflow results. If the biosafety cabinet is connected to a remote (building) exhaust
system, ensure the remote exhaust system is on.

Primary Method

NSF/ANSI Standard 49 now requires the Primary Method for determining inflow be
utilized unless there is less than 41 inches (104 cm) clearance between the front of the
biosafety cabinet and the nearest physical, permanent obstruction.

The Primary Method requires the following:

 DIM (Direct Inflow Meter), such as a ShortridgeTM
 Labconco Holder Bracket Assembly (Catalog Number 3836405), shown below in

Figure 10-28.

 Thin, rigid plastic (or similar material) blocking plates, to close off front work

opening on either side of the DIM so all inflow air is directed through the DIM.

 Removable tape to secure the plastic blocking plates.

1. Start the blower(s), ensure the sash is at the correct operating height.

2. Attach the Labconco Holder Bracket Assembly to the front grille centered left-to­right on the biosafety cabinet. Loosen the two Thumbnuts to create a gap
between the two plates.

3. Place the DIM’s skirt frame into the gap between the two plates on the Bracket
Assembly.

Figure 10-28

Two pins insert into front-most
row of holes on the front grille

Thumbnuts

77

4. Lower the sash until the skirt frame rests on the inside of the glass and remains
in place. A tall stand may be used to support the opposite end of the DIM if
desired.

5. If a sash alarm occurs, press [OK/MUTE].

6. Install a blocking plate on either side of the DIM, and tape them securely on all
four sides. All air should enter through the DIM, seal gaps around blocking
plates completely.

7. Turn on the DIM, set it to read airflow volume continuously.

8. Take 5 readings over a 1 minute period. Average the results.

9. Compare results with column “Inflow Volume Range” based on Type and model
shown in Table 10-7.

10. If the Inflow Volume is not within the acceptance criteria range, adjust the
biosafety cabinet according to Adjusting the Blower Speed and/or Adjusting the

Internal Damper found in Section 9: Calibration.

78

Acceptance Criteria

Inflow Volume shall be in the range specified in Table 10-7 column “Inflow Volume
Range”.

Type
A2 & B2
Cabinet

Width

Sash

Height

Inflow

Average1

Sash Open Area

Inflow

Volume

Inflow Volume Range

inches

FPM (m/s)

ft2

m

2

CFM

CFM

m3/hr

3-foot

8

105

2.03

0.19

213

203-223

345-379

9

105

2.28

0.21

240

228-251

387-426

10

105

2.53

0.24

266

253-278

430-472

4-foot

8

105

2.69

0.25

283

269-296

457-503

9

105

3.03

0.28

318

303-333

515-566

10

105

3.37

0.31

354

337-371

573-630

12

105

4.04

0.38

424

404-444

686-754

5-foot

8

105

3.36

0.31

353

336-370

571-629

9

105

3.78

0.35

397

378-416

642-707

10

105

4.20

0.39

441

420-462

714-785

12

105

5.05

0.47

530

505-555

858-943

6-foot

8

105

4.03

0.37

423

403-443

685-753

9

105

4.53

0.42

476

453-498

770-846

10

105

5.03

0.47

529

503-553

855-940

12

105

6.06

0.56

636

606-666

1030-1132

Type C1

Cabinet

Width

Sash

Height

Inflow

Average1

Sash Open Area

Inflow

Volume

Inflow Volume Range

inches

FPM (m/s)

ft2

m

2

CFM

CFM

m3/hr

4-foot

8

105

2.69

0.25

283

269-296

457-503

10

105

3.37

0.31

354

337-371

573-630

6-foot

8

105

4.03

0.37

423

403-443

685-753

10

105

5.03

0.47

529

503-553

855-940

1. Tolerance for Average Inflow Velocity = +/- 5 FPM (+/- 0.03 m/s)

Table 10-7

79

Secondary Method

NSF/ANSI Standard 49 now requires the Primary Method for determining inflow be
utilized unless there is less than 41 inches (104 cm) clearance between the front of the
biosafety cabinet and the nearest physical, permanent obstruction. If there is less than
41 inches (104 cm) between the front of the biosafety cabinet and the nearest physical,
permanent obstruction, the Secondary Method for measuring inflow may be used.

The Secondary Method requires the following:

 Thermal Anemometer
 Labconco Certifier Kit (Catalog Number 3858400), which contains:

o Labconco Holder Bracket Assembly (Catalog Number 3836405)
o Left and Right Sash Stops, to position sash at correct restricted height

 3-ft through 6-ft (Logic Generation)
 3-ft through 6-ft (Logic+ Generation)

1. Based on the width of biosafety cabinet under test, identify the correct set of
Sash Stops (1 right and 1 left). Find the set of Second Generation Sash Stops,

which have a ‘+’ engraved under the width number. For example, if a 4-ft model
is under test, select the two Sash Stops engraved with a ‘4 +’.

2. Place the thermal anemometer probe into the Labconco Holder Bracket
Assembly. For convenience, the 6-ft Sash Stops have two lines to position the tip
of the thermal anemometer correctly in the Holder. The thermal anemometer
should be positioned such that the distance from the bottom of the Holder
Bracket to the center of the anemometer element equals the value in column
“Probe Distance” in Table 10-8 based on model width. 3-ft and 6-ft model widths
require the 3.25 inch (83 mm) position; 4-ft and 5-ft model widths require the 4.00
inch (102 mm) position. See Figures 10-29 (Sash Stop) and 10-30 (direct
measurement with a ruler) below. Tighten thumbnuts on Holder to secure probe.

Figure 10-29

Figure 10-30

Anemometer

Element Center

6-ft Sash

Stop

Labconco

Holder

(3836405)

Ruler

measure

80

3. Insert the appropriate left and right stop into the recess in each corner post, as
shown in Figure 10-31. Lower the sash until it contacts the Sash Stops.

4. Locate the single row of holes at the front-most edge of the grille, see Figure 10-

31.

5. Mark the 6th hole from the left side wall and subsequently mark every 9th hole
until the number of test points marked equals the width of the biosafety cabinet in
feet. For example, a 5-ft width model should have 5 test points marked.

6. Mark the 6th hole from the right side wall and subsequently mark every 9th hole
until the number of test points marked equals the width of the biosafety cabinet in
feet. For example, a 5-ft width model should have 5 test points marked. All test
points should be marked on the grille. See Figure 10-32 for reference.

Figure 10-31

Sash Stop

(Same opposite end)

Row of Holes

Figure 10-32

Test Point marks

81

7. Place the thermal anemometer, now secured to the Labconco Holder, onto the
grille. The two pins in the Holder will drop into two holes on the grille. The
thermal anemometer probe should line up with the test point mark made earlier.
Start by placing the anemometer and Holder at the far left test point mark. See
Figure 10-33 for reference.

11. Start the blower(s), and let the cabinet operate for at least 5 minutes. After the
warm-up period expires, the biosafety cabinet will display a sash alarm. This is
because the sash is at the restricted height required for this test. Press

[OK/MUTE] to silence the alarm for 5 minutes.

12. This test requires the anemometer to provide highly accurate results. Establish
the necessary correction factor to the thermal anemometer from its calibration
performance test in a calibrated wind tunnel. This should be available from the
calibration institution or company used to calibrate the thermal anemometer.

Calculate the thermal anemometer manufacturer’s recommended correction

factor(s) for conditions of temperature, humidity, barometric pressure, and
altitude.

13. Set the anemometer’s time constant to 10-15 seconds for greater data stability.

14. Take one velocity reading at each of the marked test points.

Figure 10-33

Thermal anemometer

probe in Holder

82

15. Average all the readings. Apply the wind tunnel correction and local condition
corrections factors to the average velocity reading.

16. Multiply the average inflow velocity by the column “Correction Factor” based on
Type and model in Table 10-8.

Type
A2 & B2
Cabinet

Width

Sash

Height

Sash

Stop

Template

Probe

Distance

# of Test

Points

Correction

Factor

Corrected Inflow

Volume Range

inches

inches

(mm)

CFM

m3/hr

3-foot

8

3+

4 (102)

6

0.95

203-223

345-379

9

3+

4 (102) 6 1

228-251

387-426

10

3+

4 (102)

6

0.95

253-278

430-472

4-foot

8

4+

3.25 (83)

8

1

269-296

457-503

9

4+

3.25 (83)

8

1

303-333

515-566

10

4+

3.25 (83)

8

1

337-371

573-630

12

4+

3.25 (83)

8

1.08

404-444

686-754

5-foot

8

5+

3.25 (83)

10

1

336-370

571-629

9

5+

3.25 (83)

10

1

378-416

642-707

10

5+

3.25 (83)

10

1

420-462

714-785

12

5+

3.25 (83)

10

1

505-555

858-943

6-foot

8

6+

4 (102)

12

1.83

403-443

685-753

9

6+

4 (102)

12

1.83

453-498

770-846

10

6+

4 (102)

12

1.83

503-553

855-940

12

6+

4 (102)

12

2.0

606-666

1030-1132

Type C1

Cabinet

Width

Sash

Height

Sash

Stop

Template

Probe

Distance

# of Test

Points

Correction

Factor

Corrected Inflow

Volume Range

inches

inches

(mm)

CFM

inches

4-foot

8

4+

3.25 (83)

8

1.08

269-296

457-503

10

4+

3.25 (83)

8

1.08

337-371

573-630

6-foot

8

6+

4 (102)

12

2

403-443

685-753

10

6+

4 (102)

12

2

503-553

855-940

Table 10-8

83

17. Compare the inflow volume in CFM calculated in step #15 with the value in
column “Corrected Inflow Volume Range” based on Type and model shown in
Table 10-8.

18. If the Inflow Volume is not within the acceptance criteria range, adjust the
biosafety cabinet according to Adjusting the Blower Speed and/or Adjusting the
Internal Damper found in Section 9: Calibration.

Acceptance Criteria

Inflow Volume shall be in the range specified in Table 10-8 column “Corrected Inflow
Volume Range”.

84

Work Area Air Cleanliness Test (optional)

Air cleanliness is a measurement of all particles (greater than or equal to 0.5 micron in
size) in the air. This is not an NSF Standard 49 test. However, if the end user would
like to verify air cleanliness in the work area, follow these instructions:

1. Mark test points at the geometric center of the work surface, and midway
between the center test point and each side wall.

2. Place a particle counter pickup at a height of 16 inches (41 cm) above the work
surface, pointing up into the airflow. Place the particle counter pickup at each of
the three marked test points, and take one sample at each test point of 1.0 ft3 of
air. See Figure 10-34 for reference.

Note: if sampling 1.0 m3 instead of 1.0 ft3, see Acceptance Criteria below for
passing threshold.

3. Average the particle count results from each of the three test points.

Acceptance Criteria

The average particle count is less than 100 particles 0.5 micron and larger per cubic
foot, or less than 3,520 particles 0.5 micron and larger per cubic meter.

Figure 10-34

Particle

Counter

Pickup

85

Lighting Test (optional)

The light intensity should be measured as described in the current NSF/ANSI Standard

49 Annex F. To test the light intensity at the biosafety cabinet’s work surface, follow

these instructions:

Note: A light meter that is color- and cosign-corrected must be used for accurate
results.

1. Mark test points on the work surface as follows:

a. Mark the front-to-back centerline of the work surface
b. Starting 6 inches (15.2 cm) from the left side wall, mark a test point on the

front-to-back centerline.

c. Continue marking additional test points along the centerline at 12 inch

(30.5 cm) increments until the left-to-right center of the work surface is

reached.
d. Repeat steps b and c starting from the right side wall.
e. All test points are now marked.

2. With the biosafety cabinet lights off, take a background reading with the light
meter. It should be 15 foot-candles (161 lux) or less.

3. Turn on the lights of the biosafety cabinet. Wait 1 minute.

4. Place the light meter sensor at each of the marked test points and take a
reading.

5. Average all test point readings.

Acceptance Criteria

The average light level shall be no less than 45 foot-candles (484 lux) greater than the
background light level reading.

86

Noise Test (optional)

The noise (sound pressure) should be measured as described in the current NSF/ANSI
Standard 49 Annex F. To test the biosafety cabinet’s noise level, follow these
instructions:

1. Establish the sound meter location, as follows:

a. On the left-to-right centerline of the biosafety cabinet
b. 14 inches (35.6 cm) above the work surface
c. 12 inches (30.5 cm) from the front of the biosafety cabinet

2. With the biosafety cabinet’s blower(s) off, take a background reading with the
sound meter. It should be less than 55 dbA.

3. Start the blower(s) on the biosafety cabinet.

4. Place the sound meter at the location defined in Step 1, take a sound reading.

Acceptance Criteria

The noise (sound pressure) shall be no more than 67 dbA when the biosafety cabinet is
new, and no more than 73 dbA when the filters are loaded with particulate.

Vibration Test (optional)

The vibration level at the work surface should be measured as described in the current
NSF/ANSI Standard 49 Annex F. To test the biosafety cabinet’s work surface vibration
level, follow these instructions:

Note: The vibration meter must have a range of 20-20,000 Hz and provide an output in
displacement to proceed with this test.

1. Mark the geometric center of the work surface.

2. Place the vibration meter probe at this point, and with the biosafety cabinet’s
blower(s) off, take a background reading.

3. Start the biosafety cabinet’s blower(s). Wait 1 minute.

4. Take a reading at the same work surface location.

5. Subtract the reading with the blower(s) on from the background reading.

Acceptance Criteria

Net vibration shall be less than 0.0002 inches (5 x 10-6 m) when the biosafety cabinet is
new.

87

UV Light Intensity Test (optional)

To test the biosafety cabinet’s UV light intensity level at the work surface, follow these
instructions:

Note: The UV radiometer must measure light at a wavelength of 254 nm to proceed
with this test.

1. Mark the geometric center of the work surface.

2. Place the radiometer probe at this point, and with the biosafety cabinet’s
blower(s), light, and UV light off, take a background reading.

3. Close the sash. Turn on the biosafety cabinet’s UV Light. Wait 5 minutes.

4. Take a reading at the same work surface location.

5. Subtract the reading with the UV Light on from the background reading.

Acceptance Criteria

Net UV light intensity shall be at least 100 uW-cm2 or greater when the biosafety
cabinet’s UV light lamp is new.

88

Ground Fault Circuit Interrupter (GFCI) Test

To test the biosafety cabinet’s internal GFCI outlets, follow these instructions:
This test may only be applied on 100-115v models. 208-230v models do not contain

GFCI outlets internal to the biosafety cabinet.

Note: The GFCI tester must be capable of simulating a fault of 3mA.

1. Place the tester into any of the biosafety cabinet’s outlets. The left side outlet is
a standard (non-GFCI) outlet, but it is wired to the LOAD side of the GFCI outlet
on the right side wall. It will respond the same; however, to reset power to the
outlets after the test, the Reset Button on the right side outlet needs to be
pressed.

2. Press the test button on the GFCI tester. The indicator lights on the tester should

indicate the outlet is inactive, and the GFCI outlet’s power indicator LED should

be off. See Figure 10-35 for reference.

3. Reset the GCI by pressing the RESET button on the right side outlet. The tester

should indicate power is correctly present at the outlet, and the GFCI outlet’s

power indicator LED should be on.

Acceptance Criteria

The GFCI tester and GFCI outlet in the biosafety cabinet respond as indicated in steps
2 and 3.

Figure 10-35

GFCI Reset Button

GFCI Test Button

GFCI Power

Indicator LED

The GFCI outlet’s
RESET and TEST
buttons may be white,
or red/black (as shown
in this figure)

89

11: QuickCharts

The QuickCharts section provides a central location for all critical data and
specifications of all biosafety cabinet types and models. The data included on these
charts includes: downflow, inflow, building exhaust system requirements, HEPA filter
specifications and leak test parameters.

The charts are provided in imperial (English) units only. Much of the data is located in
other areas of this document as they relate to specific calibration and certification
procedures and is provided in metric units in those locations. The charts are separated
by biosafety cabinet type: A2, B2, and C1.

90

QuickChart – Type A2 3-ft & 4-ft

Type A2 Cabinet Width

3-ft

4-ft

Sash Opening (inches)

8 9 10 8 9

10

12

Starting Serial #

1905_

1905_

1905_

1905_

1905_

1905_

2006_

Downflow Data

Nominal Avg. Downflow (FPM)

55+/-5

55+/-5

55+/-5

55+/-5

55+/-5

55+/-5

60+/-5

Grid points (rows x columns)

21 (3x7)

21 (3x7)

21 (3x7)

24 (3x8)

24 (3x8)

24 (3x8)

24 (3x8)

Grid distance from back & sides

6.0

6.0

6.0

6.0

6.0

6.0

6.0

Distance between rows

5.75

5.75

5.55

5.75

5.75

5.55

5.44

Distance between columns

4.08

4.08

4.08

5.21

5.21

5.21

5.21

Inflow/Exhaust Data

Nominal Average Inflow (FPM)

105+/-5

105+/-5

105+/-5

105+/-5

105+/-5

105+/-5

105+/-5

Sash Open Area (Sq. Ft)

2.03

2.28

2.53

2.69

3.03

3.37

4.04

Nominal Avg. Inflow Vol. (CFM)

213

240

266

283

318

354

424

Avg. Inflow Vol. Range (CFM)

203-223

228-251

253-278

269-296

303-333

337-371

404-444

Secondary Inflow Data

Sash Opening Template

1

3+

3+

3+

4+

4+

4+

4+

Sensor distance (inches)2

4 4 4

3.25

3.25

3.25

3.25

# of Test points

6 6 6 8 8 8 8

Avg. Inflow Vel. Range (FPM)

214-235

228-251

266-293

269-296

303-333

337-371

374-411

Correction Factor (CF)

0.95 1 0.95 1 1 1 1.08

Corrected Inflow Volume (CIV)

(Avg. velocity x CF)

203-223

228-251

253-278

269-296

303-333

337-371

404-444

HEPA Filter Leak Test Data

Air Displacement (CFM)

550

570

600

725

760

800

900

Laskin Nozzles needed

1 1 1 2 2 2 3

Theoretical aerosol conc. (ug/l)3

25

24

23

37

36

34

30

Actual aerosol conc. (ug/l)4

14

12

12

21

18

18

15

Supply HEPA Data

Labconco P/N

3838400

3838400

3838400

3838401

3838401

3838401

3838401

Width x Depth x Height (in.)5

36x18x3.06

36x18x3.06

36x18x3.06

48x18x3.06

48x18x3.06

48x18x3.06

48x18x3.06

Performance (CFM)

335

335

335

445

445

445

445

Pressure Drop (in. H2O)

.62+/-.1”

.62+/-.1”

.62+/-.1”

.5+/-.1”

.5+/-.1”

.5+/-.1”

.5+/-.1”

Exhaust HEPA Data

Labconco P/N

3838500

3838500

3838500

3838501

3838501

3838501

3838501

Width x Depth x Height (in.) 5

18x18x5.88

18x18x5.88

18x18x5.88

26x18x5.88

26x18x5.88

26x18x5.88

26x18x5.88

Performance (CFM)

266

266

266

354

354

354

354

Performance (in. H2O)

.25-.35”

.25-.35”

.25-.35”

.2-.37”

.2-.37”

.2-.37”

.2-.37”

LED Lamp Data (2 each)7

Labconco P/N

1297503

1297503

1297503

1297504

1297504

1297504

1297504

LED Lamps (Direct Drive)

12T8-36

12T8-36

12T8-36

15T8-48

15T8-48

15T8-48

15T8-48

Color (oK)

4000

4000

4000

4000

4000

4000

4000

Lumens

1200

1200

1200

1850

1850

1850

1850

Glass Type

Frosted

Frosted

Frosted

Frosted

Frosted

Frosted

Frosted

Ventus Canopy Data

Labconco Canopy P/N

3889200

3889200

3889200

3889201

3889201

3889201

3889201

Canopy Slot Area (ft2)

.12

.12

.12

.12

.12

.12

.12

Nominal Canopy Inflow (CFM)

50

50

50

50

50

50

50

Nominal Canopy Slot Velocity (FPM)

240-260

240-260

240-260

240-260

240-260

240-260

240-260

Canopy Vacuum (in. H2O)

0.15

0.15

0.15

0.15

0.15

0.15

0.15

DIM Total Exhaust Volume

Cabinet + Canopy (CFM)8

263

290

316

333

369

404

474

Traverse Total Exhaust Volume

Cabinet + Canopy (CFM)9

289

319

348

366

405

444

521

Volume of Hull

Cubic Feet

29

29

29

39

39

39

39

91

QuickChart – Type A2 5-ft & 6-ft

Type A2 Cabinet Width

5-ft

6-ft

Sash Opening (inches)

8 9 10

12 8 9

10

12

Starting Serial #

1905_

1905_

1905_

2006_

1905_

1905_

1905_

2006_

Downflow Data

Nominal Avg. Downflow (FPM)

55+/-5

55+/-5

55+/-5

60+/-5

55+/-5

55+/-5

55+/-5

60+/-5

Grid points (rows x columns)

30(3x10)

30(3x10)

30(3x10)

30(3x10)

36(3x12)

36(3x12)

36(3x12)

36(3x12)

Grid distance from back & sides

6.0

6.0

6.0

6.0

6.0

6.0

6.0

6.0

Distance between rows

5.75

5.75

5.55

5.44

5.75

5.75

5.55

5.44

Distance between columns

5.39

5.39

5.39

5.39

5.50

5.50

5.50

5.50

Inflow/Exhaust Data

Nominal Average Inflow (FPM)

105+/-5

105+/-5

105+/-5

105+/-5

105+/-5

105+/-5

105+/-5

105+/-5

Sash Open Area (Sq. Ft)

3.36

3.78

4.20

5.05

4.03

4.53

5.03

6.06

Nominal Avg. Inflow Vol. (CFM)

353

397

441

530

423

476

529

636

Avg. Inflow Vol. Range (CFM)

336-370

378-416

420-462

505-555

403-443

453-498

503-553

606-666

Secondary Inflow Data

Sash Opening Template

1

5+

5+

5+

5+

6+

6+

6+

6+

Sensor distance (inches)2

3.25

3.25

3.25

3.25 4 4 4 4

# of Test points

10

10

10

10

12

12

12

12

Avg. Inflow Vel. Range (FPM)

336-370

378-416

420-462

505-556

220-242

248-272

275-302

303-333

Correction Factor (CF)

1 1 1 1 1.83

1.83

1.83

2.0

Corrected Inflow Volume (CIV)

(Avg. velocity x CF)

336-370

378-416

420-462

505-555

403-443

453-498

503-553

606-666

HEPA Filter Leak Test Data

Air Displacement (CFM)

900

950

1000

1135

1100

1140

1200

1380

Laskin Nozzles needed

2 2 2 3 2 2 2 3 Theoretical aerosol conc. (ug/l)3

30

28

27

24

25

24

23

20

Actual aerosol conc. (ug/l)4

17

14

14

13

17

15

15

10

Supply HEPA Data

Labconco P/N

3838402

3838402

3838402

3838402

3838403

3838403

3838403

3838403

Width x Depth x Height (in.)5

60x18x3.06

60x18x3.06

60x18x3.06

60x18x3.06

72x18x3.06

72x18x3.06

72x18x3.06

72x18x3.06

Performance (CFM)

555

555

555

555

665

665

665

665

Pressure Drop (in. H2O)

.5+/-.1”

.5+/-.1”

.5+/-.1”

.5+/-.1”

.38-.5”

.38-.5”

.38-.5”

.38-.5”

Exhaust HEPA Data

Labconco P/N

3838502

3838502

3838502

3838502

3838503

3838503

3838503

3838503

Width x Depth x Height (in.) 5

36x18x5.88

36x18x5.88

36x18x5.88

36x18x5.88

48x18x5.88

48x18x5.88

48x18x5.88

48x18x5.88

Performance (CFM)

441

441

441

441

529

529

529

529

Performance (in. H2O)

.2-.37”

.2-.37”

.2-.37”

.2-.37”

.2-.37”

.2-.37”

.2-.37”

.2-.37”

LED Lamp Data (2 each)7

Labconco P/N

1297505

1297505

1297505

1297505

1297506

1297506

1297506

1297506

LED Lamps (Direct Drive)

24T8-60

24T8-60

24T8-60

24T8-60

32T8-72

32T8-72

32T8-72

32T8-72

Color (oK)

4000

4000

4000

4000

4000

4000

4000

4000

Lumens

2400

2400

2400

2400

3200

3200

3200

3200

Glass Type

Frosted

Frosted

Frosted

Frosted

Frosted

Frosted

Frosted

Frosted

Ventus Canopy Data

Labconco Canopy P/N

3889202

3889202

3889202

3889202

3889203

3889203

3889203

3889203

Canopy Slot Area (ft2)

.12

.12

.12

.12

.12

.12

.12

.12

Nominal Canopy Inflow (CFM)

50

50

50

50

50

50

50

50

Nominal Canopy Slot Velocity (FPM)

240-260

240-260

240-260

240-260

240-260

240-260

240-260

240-260

Canopy Vacuum (in. H2O)10

0.15

0.15

0.15

0.15

0.15

0.15

0.15

0.15

DIM Total Exhaust Volume

Cabinet + Canopy (CFM)8

403

447

491

580

473

526

579

686

Traverse Total Exhaust Volume

Cabinet + Canopy (CFM)9

443

492

540

638

520

579

637

755

Volume of Hull

Cubic Feet

49

49

49

49

58

58

58

58

See footnotes on following page

92

Type A2 Footnotes

Type and Model Identification

 The primary serial tag is on the lower right edge of the front dress panel.
 The secondary serial tag is on the front of the electronics module on the cabinet’s top right side.
 The first two digits of the serial number are the year of production; the next two are the month. The next 5

digits are the sequence of production, and the letter following the serial number is the revision level of the
cabinet.

 See Section 3:Catalog Number Configurator to discern Type, Model, and Sash Height from Catalog Number

found on the serial tag.

Downflow Test Specifications

 All models are classified as uniform downflow.
 All tests performed as described in the current NSF/ANSI Standard 49.
 UV Lamp, IV bar and all other accessories must be removed before measuring downflow.

Inflow Test Specifications

 All tests performed as described in the current NSF/ANSI Standard 49.

Secondary Inflow Test Specifications

 Must use Labconco holder P/N 3836405 to perform this test properly.

1. Use the appropriate template included in Certifier Kit# 3858400.

2. Measured from bottom edge of the probe holder to center of the thermal anemometer element.

 Locate the single row of holes at the front of the grille. Mark the 6th hole from the side wall and subsequent

test points every 9 holes until the number of test points marked equals the width of the cabinet in feet (i.e. 3­foot cabinet, mark the first 3 points). Repeat for the opposite side.

HEPA Filter Leak Test Specifications

3. Based on mineral oil.

4. The actual aerosol concentration is what was observed during testing.

 Aerosol generator should be placed in the left rear corner of the work area, pointing at the rear grille.
 For uncontaminated units, the upstream concentration can be sampled from the tube located under the work

surface.

Supply and Exhaust HEPA Filter Specifications

5. Without gasket

Motor / Blower Specifications

 Each motor must be programmed by Labconco for the appropriate width cabinet. The PWM setting will

fluctuate depending on local temperature and pressure.

LED Lamp Specifications

7. THIS PRODUCT USES DIRECT DRIVE T8 LED LAMPS INSTEAD OF FLUORESCENT LAMPS. THERE
IS NO BALLAST; LINE VOLTAGE IS SUPPLIED TO THE LAMP SOCKETS. DO NOT INSTALL
FLUORESCENT LAMPS! FOR REPLACEMENT LED LAMPS, CONTACT LABCONCO PRODUCT
SERVICE DEPARTMENT.

Canopy & Remote Exhaust System Data

8. DIM = Direct Inflow Meter

9. Measured as per ASHRAE methodology for measuring air volume in round ducts. These values are
approximations, as the calculated traverse volume value may vary depending on sampling location, exhaust
system, and methodology.

10. Measured at the exhaust transition sampling point, relative to atmosphere.

UV lamp Specifications

 For all models, the UV lamp model is G30T8. Labconco p/n is 1271300.
 For all 115/230 VAC models, the ballast assembly is Labconco p/n 3829901.
 For all 100 VAC models, the ballast assembly is Labconco p/n 3830600.

93

QuickChart – Type B2

Type B2 Cabinet Width

4-ft

6-ft

Sash Opening (inches)

8 8 Starting Serial #

1905_

1905_

Downflow Data

Nominal Avg. Downflow (FPM)

55+/-5

55+/-5

Grid points (rows x columns)

24 (3x8)

36(3x12)

Grid distance from back & sides

6.0

6.0

Distance between rows

5.75

5.75

Distance between columns

5.21

5.50

Inflow/Exhaust Data

Nominal Average Inflow (FPM)

105+/-5

105+/-5

Sash Open Area (Sq. Ft)

2.69

4.03

Nominal Avg. Inflow Vol. (CFM)

283

423

Avg. Inflow Vol. Range (CFM)

269-296

403-443

Secondary Inflow Data

Sash Opening Template

1

4+

6+

Sensor distance (inches)2

3.25 4 # of Test points

8

12

Avg. Inflow Vel. Range (FPM)

269-296

220-242

Correction Factor (CF)

1

1.83

Corrected Inflow Volume (CIV)

(Avg. velocity x CF)

269-296

403-443

Supply HEPA Filter Leak Test Data

Air Displacement (CFM)

445

665

Laskin Nozzles needed

1 1 Theoretical aerosol conc. (ug/l)3

30

15

Actual aerosol conc. (ug/l)4

20

12

Supply HEPA Data

Labconco P/N

3838401

3838403

Width x Depth x Height (in.)5

48x18x3.06

72x18x3.06

Performance (CFM)

445

665

Pressure Drop (in. H2O)

.5+/-.1”

.38-.5”

Exhaust HEPA Filter Leak Test Data

Air Displacement (CFM)

723

1083

Laskin Nozzles needed

2 2 Theoretical aerosol conc. (ug/l)3

38

25

Actual aerosol conc. (ug/l)4

21

17

Exhaust HEPA Data

Labconco P/N

3438501

3438503

Width x Depth x Height (in.)5

26x18x8.08

48x18x8.08

Performance (CFM)

720

1100

Performance (in. H2O)

.48+/-.07”

.37+/-.07”

LED Lamp Data (2 each)7

Labconco P/N

1297504

1297506

LED Lamps (Direct Drive)

15T8-48

32T8-72

Color (oK)

4000

4000

Lumens

1850

3200

Glass Type

Frosted

Frosted

Remote Exhaust System Data

DIM8 Total Exhaust Airflow (CFM)9

723

1083

Traverse Total Exhaust Airflow (CFM)10

852

1265

Recommended Duct Vacuum (in. H

2

O)

11

1.8

2.2

Exhaust (Inflow) Alarm Data

Inflow Alarm Set Point (CFM)

210 +/-5

315 +/-5

Volume of Hull

Cubic Feet

39

58

See footnotes on following page

94

Type B2 Footnotes

Type and Model Identification

 The primary serial tag is on the lower right edge of the front dress panel.
 The secondary serial tag is on the front of the electronics module on the cabinet’s top right side.
 The first two digits of the serial number are the year of production; the next two are the month. The next 5

digits are the sequence of production, and the letter following the serial number is the revision level of the
cabinet.

 See Section 3:Catalog Number Configurator to discern Type, Model, and Sash Height from Catalog Number

found on the serial tag.

Downflow Test Specifications

 All models are classified as uniform downflow.
 All tests performed as described in the current NSF/ANSI Standard 49.
 UV Lamp, IV bar and all other accessories must be removed before measuring downflow.

Inflow Test Specifications

 All tests performed as described in the current NSF/ANSI Standard 49.

Secondary Inflow Test Specifications

 Must use Labconco holder P/N 3836405 to perform this test properly.

1. Use the appropriate template included in Certifier Kit# 3858400.

2. Measured from bottom edge of the probe holder to center of the thermal anemometer element.

 Locate the single row of holes at the front of the grille. Mark the 6th hole from the side wall and subsequent

test points every 9 holes until the number of test points marked equals the width of the cabinet in feet (i.e. 4­foot cabinet, mark the first 4 points). Repeat for the opposite side.

HEPA Filter Leak Test Specifications

3. Based on mineral oil.

4. The actual aerosol concentration is what was observed during testing.

 Aerosol generator should be placed in the left rear corner of the work area, pointing at the rear grille.
 For uncontaminated units, the upstream concentration for the Supply HEPA only can be sampled from the

tube located under the work surface.

Supply and Exhaust HEPA Filter Specifications

5. Without gasket

Motor / Blower Specifications

 Each motor must be programmed by Labconco for the appropriate width cabinet. The PWM setting will

fluctuate depending on local temperature and pressure.

LED Lamp Specifications

7. THIS PRODUCT USES DIRECT DRIVE T8 LED LAMPS INSTEAD OF FLUORESCENT LAMPS. THERE
IS NO BALLAST; LINE VOLTAGE IS SUPPLIED TO THE LAMP SOCKETS. DO NOT INSTALL
FLUORESCENT LAMPS! FOR REPLACEMENT LED LAMPS, CONTACT LABCONCO PRODUCT
SERVICE DEPARTMENT.

Remote Exhaust System Data

8. DIM = Direct Inflow Meter

9. Total Exhaust Volume required from remote (building) exhaust system.

10. Measured as per ASHRAE methodology for measuring air volume in round ducts. These values are
approximations, as the calculated traverse volume value may vary depending on sampling location, exhaust
system, and methodology.

11. Measured at the exhaust transition sampling point, relative to atmosphere.

UV lamp Specifications

 For all models, the UV lamp model is G30T8. Labconco p/n is 1271300.
 For all 115/230 VAC models, the ballast assembly is Labconco p/n 3829901.
 For all 100 VAC models, the ballast assembly is Labconco p/n 3830600.

95

QuickChart – Type C1

Type C1 Cabinet Width

4-ft

6-ft

Sash Opening (inches)

8

10 8 10

Starting Serial #

2006_

2006_

2006_

2006_

Downflow Data

Nominal Avg. Downflow (FPM)

65+/-5

65+/-5

55+/-5

55+/-5

Grid points (rows x columns)

24 (3x8)

24 (3x8)

36(3x12)

36(3x12)

Grid distance from back & sides

6.0

6.0

6.0

6.0

Distance between rows

5.75

5.55

5.75

5.55

Distance between columns

5.21

5.21

5.50

5.50

Inflow/Exhaust Data

Nominal Average Inflow (FPM)

105+/-5

105+/-5

105+/-5

105+/-5

Sash Open Area (Sq. Ft)

2.69

3.37

4.03

5.03

Nominal Avg. Inflow Vol. (CFM)

283

354

423

529

Avg. Inflow Vol. Range (CFM)

269-296

337-371

403-443

503-553

Secondary Inflow Data

Sash Opening Template

1

4+

4+

6+

6+

Sensor distance (inches)2

3.25

3.25 4 4

# of Test points

8 8 12

12

Avg. Inflow Vel. Range (FPM)

249-274

312-344

202-222

252-277

Correction Factor (CF)

1.08

1.08 2 2

Corrected Inflow Volume (CIV)

(Avg. velocity x CF)

269-296

337-371

403-443

503-553

Supply HEPA Filter Leak Test Data

Air Displacement (CFM)

520

520

665

665

Laskin Nozzles needed

1 1 1 1 Theoretical aerosol conc. (ug/l)3

26

26

20

20

Actual aerosol conc. (ug/l)4

13

13

12

12

Supply HEPA Data

Labconco P/N

3838401

3838401

3838403

3838403

Width x Depth x Height (in.)5

48x18x3.06

48x18x3.06

72x18x3.06

72x18x3.06

Performance (CFM)

445

445

665

665

Pressure Drop (in. H2O)

.5+/-.1”

.5+/-.1”

.38-.5”

.38-.5”

Exhaust HEPA Filter Leak Test Data

Air Displacement (CFM)

283

354

423

529

Laskin Nozzles needed

1 1 1 1 Theoretical aerosol conc. (ug/l)3

48

38

32

26

Actual aerosol conc. (ug/l)4

28

22

19

15

Exhaust HEPA Data

Labconco P/N

3838501

3838501

3838503

3838503

Width x Depth x Height (in.) 5

26x18x5.88

26x18x5.88

48x18x5.88

48x18x5.88

Performance (CFM)

353

353

529

529

Performance (in. H2O)

.2-.35”

.2-.35”

.2-.35”

.2-.35”

LED Lamp Data (2 each)7

Labconco P/N

1297504

1297504

1297506

1297506

LED Lamps (Direct Drive)

15T8-48

15T8-48

32T8-72

32T8-72

Color (oK)

4000

4000

4000

4000

Lumens

1850

1850

3200

3200

Glass Type

Frosted

Frosted

Frosted

Frosted

Remote Exhaust System Data12

DIM Total Exhaust Volume

Cabinet + Canopy (CFM)8

323

404

473

579

Traverse Total Exhaust Volume Cabinet

+ Canopy (CFM)9

366

444

520

637

IRV11 Slot Area (ft2)

.12

.12

.12

.12

Nominal IRV Inflow (CFM)

50

50

50

50

Nominal IRV Slot Velocity (FPM)

240-260

240-260

240-260

240-260

Recommended Duct Vacuum (in. H2O)10

0.3

0.3

0.3

0.3

Volume of Hull

Cubic Feet

39

39

58

58

See footnotes on following page

96

Type C1 Footnotes

Type and Model Identification

 The primary serial tag is on the lower right edge of the front dress panel.
 The secondary serial tag is on the front of the electronics module on the cabinet’s top right side.
 The first two digits of the serial number are the year of production; the next two are the month. The next 5

digits are the sequence of production, and the letter following the serial number is the revision level of the
cabinet.

 See Section 3:Catalog Number Configurator to discern Type, Model, and Sash Height from Catalog Number

found on the serial tag.

Downflow Test Specifications

 All models are classified as uniform downflow.
 All tests performed as described in the current NSF/ANSI Standard 49.
 UV Lamp, IV bar and all other accessories must be removed before measuring downflow.

Inflow Test Specifications

 All tests performed as described in the current NSF/ANSI Standard 49.

Secondary Inflow Test Specifications

 Must use Labconco holder P/N 3836405 to perform this test properly.

1. Use the appropriate template included in Certifier Kit# 3858400.

2. Measured from bottom edge of the probe holder to center of the thermal anemometer element.

 Locate the single row of holes at the front of the grille. Mark the 6th hole from the side wall and subsequent

test points every 9 holes until the number of test points marked equals the width of the cabinet in feet (i.e. 3­foot cabinet, mark the first 3 points). Repeat for the opposite side.

HEPA Filter Leak Test Specifications

3. Based on mineral oil.

4. The actual aerosol concentration is what was observed during testing.

 Aerosol generator should be placed in the left rear corner of the work area, pointing at the rear grille.
 For uncontaminated units, the upstream concentration can be sampled from the tube located under the work

surface.

Supply and Exhaust HEPA Filter Specifications

5. Without gasket

Motor / Blower Specifications

 Each motor must be programmed by Labconco for the appropriate width cabinet. The PWM setting will

fluctuate depending on local temperature and pressure.

LED Lamp Specifications

7. THIS PRODUCT USES DIRECT DRIVE T8 LED LAMPS INSTEAD OF FLUORESCENT LAMPS. THERE
IS NO BALLAST; LINE VOLTAGE IS SUPPLIED TO THE LAMP SOCKETS. DO NOT INSTALL
FLUORESCENT LAMPS! FOR REPLACEMENT LED LAMPS, CONTACT LABCONCO PRODUCT
SERVICE DEPARTMENT.

Remote Exhaust System Data

8. DIM = Direct Inflow Meter

9. Measured as per ASHRAE methodology for measuring air volume in round ducts. These values are
approximations, as the calculated traverse volume value may vary depending on sampling location, exhaust
system, and methodology.

10. Measured at the exhaust transition sampling point, relative to atmosphere.

11. IRV = Inlet Relief Valve. Located on the front face of the Type C1 Exhaust Housing.

12. This section is only applicable to Type C1 biosafety cabinets operating in B-mode.

UV lamp Specifications

 For all models, the UV lamp model is G30T8. Labconco p/n is 1271300.
 For all 115/230 VAC models, the ballast assembly is Labconco p/n 3829901.
 For all 100 VAC models, the ballast assembly is Labconco p/n 3830600.

97

12: Advanced Service Procedures

This section provides instructions to perform advanced service procedures on the
biosafety cabinet. Such procedures include changing HEPA filters, adjusting the sash or
sash position sensors, replacing LED or UV lamps, and more.

Tools

Required

 #2 Phillips screwdriver or #2 Phillips bit (4 inches long minimum)
 Ratchet with extension
 Sockets

o 5/16-inch
o 3/8-inch
o 7/16-inch
o 1/2-inch

 3/8-inch nut driver
 1/2-inch nut driver

Optional

 Cordless screwdriver/drill
 Cordless ratchet (low profile right angle impact driver)
 Pliers (standard and needle nose)
 #10-24 Tap
 5/16-18 Tap

If a cordless screwdriver or drill is used to drive screws, reduce the torque setting to a
minimum to avoid stripping the screw head.

98

Removal of External Dress Panels

To access the HEPA filters, LED lamps, sash sensors, the LCD display, and other
service areas, the front panel and side panels will be removed. This section describes
removal of the external dress panels.

Dress Panel

1. Locate and remove the two Phillips screws that secure the front dress Panel as
shown in Figure 12-1.

2. Swing the bottom edge of the dress panel out (away from biosafety cabinet) to
clear the LED lamps underneath. Then lift the entire dress panel up to clear the
two white posts on the top right and left corners of the dress panel. The two
posts protrude down from the top flange of the dress panel.

The dress panel is heavy, and awkward to maneuver, use two persons to lift the
dress panel.

Reinstallation Notes:

1. Make sure the two white posts are fully engaged into the top of each corner post
before replacing the two screws at the bottom corners of the dress panel.

Figure 12-1

Dress Panel

Screws

Post

Post

99

Side Panels

1. Locate and remove the two Phillips screws along the front edge of each side
panel.

2. The side panels will hinge open once the screws are removed.

3. If side panel needs to be removed, position the panel so that it is almost closed.
Grasp the panel by the front and rear edges. Lift the panel up approximately 2
inches (5 cm), then pull it straight away from the end of the biosafety cabinet.

Product illustration

may not accurately

represent all models

Figure 12-2

Left

Side

Panel

Right Side Panel

Screw Location

Screw Location

100

Front Sash

The glass sash utilizes a cable and pulley system with counter weights located in the
right and left end service compartments. This section describes adjustments or repairs
to the sash or sash sensors.

Sash Level Adjustment

The sash and its two counter weights are joined together with two sash cables, one per
counter weight. The sash cables run on pulleys, which are joined together by a
common axle. Each pulley is locked to this axle with a cotter pin. This prevents the
sash from racking when raised from only one end of the sash glass. The sash should
have approximately 1/8 inch (3 mm) of lateral play or movement. Under normal
operation, the sash should remain level. Occasionally, during transport of the biosafety
cabinet, the sash may become unlevel. Follow the instructions below to resolve this
issue.

1. Lower the sash until it is fully closed. Identify which end of the sash is high.

2. Hinge open or remove the side panel on the high end of the sash. Side panel
access and removal is described earlier in this section under Removal of

External Dress Panels.

3. Using pliers, grasp the S-Hook atop the counterweight. Pull up on the
counterweight to allow some slack in the sash cable. See Figure 12-3.

4. The high end of the sash should lower against the sash stop, and the sash is
now level. Carefully lower the counterweight and sash cable back onto the
pulley.

Figure 12-3

S-Hook

Pliers

Counter
weight