Labconco Class I Enclosure User Manual

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User’s Manual

Purifier® HEPA Filtered Enclosures & Purifier® Class I Filtered Enclosures

Models

3980200, 3980201, 3980202, 3980203, 3980220, 3980221, 3980222, 3980223, 3980300, 3980301, 3980302, 3980303, 3980320, 3980321, 3980322, 3980323, 3980400, 3980401, 3980402, 3980403, 3980420, 3980421, 3980422, 3980423

Please read the User’s Manual before operating the equipment.

Warranty

Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of defective material and workmanship, provided such defect results from normal and proper use of the equipment. Glassware is not warranted from breakage when dropped or mishandled.

The warranty for Purifier® HEPA Filtered Enclosures and Purifier® Class I Filtered Enclosures will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner. Warranty is non-transferable and only applies to the owner (organization) of record.

This limited warranty covers parts and labor, but not transportation and insurance charges. In the event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This statement may be altered by a specific published amendment. No individual has authorization to alter the provisions of this warranty policy or its amendments. Lamps and filters are not covered by this warranty. Damage due to corrosion or accidental breakage is not covered.

The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change.

Returned or Damaged Goods

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damages.

The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Limitation of Liability

The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of this equipment are required to become familiar with

any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,

land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.

Contacting Labconco Corporation

If you have questions that are not addressed in this manual, or if you need technical assistance, contact Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.

Part #3905503, Rev. C

ECO J457

ORIGINAL INSTRUCTIONS

TABLE OF CONTENTS

CHAPTER 1: INTRODUCTION 1

About This Manual 2 Typographical Conventions 3

CHAPTER 2: PREREQUISITES 5

Support, Vibration & Movement Requirements 6 Location and Air Current Requirements 6 Exhaust & Blower Requirements 6 Electrical Requirements 7 Space Requirements 7

CHAPTER 3: GETTING STARTED 8

Unpacking Your Enclosure 9 Installing the Filtered Enclosure on a Supporting

Structure and Work Surface 9

Connecting to the Exhaust System (Optional) 12 Installation of HEPA Filters and Accessory Odor

Control Carbon Filters 14

Connect the Electrical Supply Source to Filtered Enclosure 15 Set the Face Velocity with Speed Control Adjustment 16 Validating the Vented Enclosure 16 Sealing the Filtered Enclosure to the Work Surface 17

CHAPTER 4: HIGH PERFORMANCE FEATURES AND

SAFETY PRECAUTIONS 18

High Performance Features 18

Components 19

Safety Precautions 24

CHAPTER 5: APPROPRIATE APPLICATIONS FOR YOUR

FILTERED ENCLOSURE 27

Routine Daily Work Procedures 27

Suitable Applications 29

HEPA Filter Applications, Suitability & Guidelines 29

Odor Control Carbon Filter Applications 30

Definition of Terms 30

Appropriate Chemicals for Odor Control Carbon Filters 31

Hazardous Misapplications for Odor Control Carbon

Filters with Volatile Chemicals 32

Chemical Carcinogen Use with Odor Control Carbon Filters 32

Prohibited Acid Use 32

CHAPTER 6: MAINTAINING YOUR FILTERED ENCLOSURE 33

Routine Maintenance Schedule 34 Decontamination 35 Determination of When to Replace HEPA Filters 35 How to Install a New HEPA Filter 35 HEPA Filter Leak Test 38

Setting the Inflow Face Velocity with the Speed Control

Adjustment 39

Calibrate and Operate the Airflow Monitor 40 Determination of When to Replace Odor Control Carbon Filters

and How to Replace 43

Calculating Odor Control Carbon Filter Life 44 Initial Certification 46 Re-Certification 46 Fluorescent Light Replacement 46 UV Light Replacement 46 Motorized Impeller Replacement 47 Speed Control Replacement 49 Trace Odor Carbon Filter Installation 49

CHAPTER 7: ACCESSORIZING & MODIFYING YOUR

FILTERED ENCLOSURE 50

CHAPTER 8: TROUBLESHOOTING & SERVICER OPERATING LOG 56

APPENDIX A: FILTERED ENCLOSURE COMPONENTS AND REPLACEMENT PARTS 60

APPENDIX B: DIMENSIONS AND EXHAUST OPTIONS 65

APPENDIX C: FILTERED ENCLOSURE SPECIFICATIONS 68

APPENDIX D: QUICK CHART FOR THE FILTERED

ENCLOSURES 69

APPENDIX E: REFERENCES ON VENTILAITON, SAFETY,

OCCUPATIONAL HAZARDS, BIOSAFETY AND DECONTAMINATION 70

CHAPTER 1

INTRODUCTION

Congratulations on your purchase of a Labconco Purifier® HEPA Filtered Enclosure or Purifier® Class I Filtered Enclosure. Your enclosure provides

personnel protection through superior containment. It is the result of Labconco’s

more than 50 years of experience in manufacturing fume hoods and more than 30 years of experience in manufacturing filtered enclosures.

These enclosures will effectively contain toxic, noxious, or biohazardous particulates when properly installed and operated. Each enclosure uses a single HEPA filter, which is rated at least 99.99% efficient for 0.3-micron particles. Additionally, an accessory Odor Control carbon filter may be added to adsorb nuisance odors from organic vapors, formaldehyde or ammonia and amines. The Purifier enclosures offer many unique features to enhance safety, performance, and energy savings. To take full advantage of them, please acquaint yourself with this manual and keep it handy for future reference. If you are unfamiliar with how high performance HEPA filtered enclosures operate, please review Chapter 4: High Performance Features and Safety Precautions before you begin working in the enclosure. Even if you are an experienced user, please review Chapter 5: Using Your HEPA Filtered Enclosure, which describes the Purifier features so that you can use the filtered enclosure efficiently. For hazardous biological applications requiring user protection but not product protection, Labconco recommends the purchase of a Class I enclosure, which includes a UV light for general surface decontamination. For general hazardous biological applications, particulates, and nuisance powders, Labconco recommends the use of the Purifier HEPA Filtered Enclosure. See chart below and contact Labconco for additional ordering information.

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Chapter 1: Introduction

No.

Application

Product

Filters

Bag-in/Bag-out

HEPA

Light

Nuisance Powders, Biohazardous Materials

Purifier HEPA Filtered Enclosure

HEPA

Decontamination

Required

Nuisance Powders, Biohazardous & Volatile Chemicals

Purifier HEPA Filtered Enclosure

HEPA

and

Carbon*

Decontamination

Required

Biohazardous Materials & Nuisance Powders

Purifier Class I Enclosure

HEPA

Decontamination

Required

Included for

Surface

Decontamination

Biohazardous Materials, Nuisance Powders & Volatile Chemicals

Purifier Class I Enclosure

HEPA

and

Carbon*

Decontamination

Required

Included for

Surface

Decontamination

* It is recommended that a Labconco product specialist review the chemical application to determine if it is suitable. Consult Chapter 5 and Chapter 6.

About This Manual

This manual is designed to help you learn how to install, use, and maintain your filtered enclosure. Instructions for installing optional equipment or accessory carbon filters on your filtered enclosure are also included.

If the unit is not operated as specified in this manual it may impair the protection provided by the unit.

Si l'unité n'est pas utilisée comme spécifié dans ce manuel il peut diminuer la protection fournie par l'unité.

Chapter 1: Introduction provides a brief overview of the filtered enclosure, explains the organization of the manual, and defines the typographical conventions used in the manual.

Chapter 2: Prerequisites explains what you need to do to prepare your site before you install the filtered enclosure. Electrical and service requirements are discussed.

Chapter 3: Getting Started contains the information you need to properly unpack, inspect, install, and certify the filtered enclosure.

Chapter 4: High Performance Features and Safety Precautions explains how the Purifier filtered enclosure operates and the appropriate precautions you should take when using it.

Chapter 5: Using Your Filtered Enclosure discusses the basic operation of how to prepare, use and shut down your filtered enclosure.

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Chapter 1: Introduction



Chapter 6: Maintaining Your Filtered Enclosure explains how to perform routine maintenance on the filtered enclosure.

Chapter 7: Accessorizing Your Filtered Enclosure explains acceptable modifications to the filtered enclosure or how to add accessories.

Chapter 8: Troubleshooting contains a table of problems you may encounter while using the filtered enclosure including the probable causes of the problems and suggested corrective actions.

Appendix A: Components and Replacement Parts contains labeled diagrams of all of the components of the filtered enclosures.

Appendix B: Dimensions and Exhaust Options contains comprehensive diagrams showing all of the dimensions for the filtered enclosures.

Appendix C: Filtered Enclosure Specifications contains the electrical requirements for filtered enclosures. Wiring diagrams are also included.

Appendix D: Quick Chart provides a table of airflow and test data for the filtered enclosures.

Appendix E: References lists the various resources available that address laboratory ventilation and biosafety.

Typographical Conventions

Recognizing the following typographical conventions will help you understand and use this manual:

 Book, chapter, and section titles are shown in italic type (e.g., Chapter 3:

Getting Started).

 Steps required to perform a task are presented in a numbered format.  Comments located in the margins provide suggestions, reminders, and

references.

 Critical information is presented in boldface type in paragraphs that are

preceded by the exclamation icon. Failure to comply with the information following an exclamation icon may result in injury to the user or permanent damage to the enclosure.

 Critical information is presented in boldface type in paragraphs that are

preceded by the wrench icon. A trained certifier or contractor should only perform these operations. Failure to comply with the information following a wrench icon may result in injury to the user or permanent damage to your hood.

 Important information is presented in capitalized type in paragraphs that are

preceded by the pointer icon. It is imperative that the information contained in these paragraphs be thoroughly read and understood by the user.

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Chapter 1: Introduction

 A number icon precedes information that is specific to a particular model of

enclosure. The 2' icon indicates the text is specific to the 2-foot wide model. The 3' icon indicates the text is specific to the 3-foot model, etc.

 CAUTION – See Manual. When this symbol is on the unit it indicates a

caution that is detailed in this manual.

 ATTENTION - Voir manuel. Lorsque ce symbole est sur l'unité, il indique une

mise en garde qui est indiqué dans ce manuel.

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CHAPTER 2

PREREQUISITES

Before you install the filtered enclosure, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power should be located near the installation site to power the filtered enclosure, and other apparatus. Additionally, the enclosure should be strategically placed in the lab to provide efficient workflow.

Carefully read this chapter to learn the requirements for your installation site:

 The support, vibration and movement requirements.  The location and air current requirements.  The exhaust and blower requirements.  The electrical power requirements.  The space requirements.

Refer to Appendix B: Purifier Dimensions for complete enclosure dimensions.

Refer to Appendix C: Purifier Specifications for complete filtered enclosure electrical and environmental conditions, specifications and requirements.

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Chapter 2: Prerequisites

Support, Vibration and Movement Requirements

At a minimum, the supporting structure usually consists of a base cabinet and chemically-resistant work surface.

 A stand or mobile bench is not recommended if the enclosure will be used for

precise weighing of powders.

Location and Air Current Requirements

The Purifier Filtered Enclosures have been designed to contain hazards by negating typical cross drafts and turbulence within the opening. However, as a precautionary safety measure and a higher level of quality management, it is recommended that the enclosure be placed in an area to avoid:

 High traffic areas where walking might cause an air disturbance or be a

nuisance.

 Overhead or wall HVAC diffusers, fans, radiators or other lab equipment

producing air currents.

 Next to doorways or windows that may be opened.

Do not position the unit so that it is difficult to operate the main disconnect device.

Ne placez pas l'appareil de sorte qu'il est difficile de faire fonctionner le dispositif principal de déconnexion.

Exhaust and Blower Requirements

The Purifier Class I Enclosure and Purifier Filtered Enclosure use an integral motorized impeller to draw room air past the operator and through the enclosure. This contaminated air is then pushed through the HEPA filter. The HEPA-filtered exhaust air is then forced out the top of the enclosure. An optional carbon filter may be installed on the downstream side of the HEPA filter to protect against nuisance odors.

The HEPA-filtered exhaust air can be recirculated into the laboratory or exhausted outside with the addition of the thimble exhaust connection and remote blower listed in Chapter 7. Data for the exhaust volume and enclosure static pressure loss are listed for each filtered enclosure model at face velocities of 60, 75, 80, 90, 100, and 105 fpm.

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Chapter 2: Prerequisites

Enclosure

Width

Model Description

Face

Velocity

(fpm)

Exhaust Volume

(CFM)

Noise

Pressure

db(A)

Max. Equivalent Resistance

of 6" Duct

(Ft)

Max.

External

Static

Pressure

2' Purifier Class I Enclosure 2' Purifier HEPA Filtered Enclosure

60 75 80

90 100 105

85 110 115 130 145 155

48-53 49-55 50-56 51-57 53-58 54-59

400 220 200 140

80 70

.20" .19" .18" .16" .13" .12"

3' Purifier Class I Enclosure 3' Purifier HEPA Filtered Enclosure

60 75 80

90 100 105

130 165 175 200 220 230

48-53 52-56 53-57 55-58 58-61 60-64

130

65 50 35 25 20

.15" .12" .11" .09" .08" .07"

4' Purifier Class I Enclosure 4' Purifier HEPA Filtered Enclosure

90 100 105

175 220 235 265 295 310

49-54 54-58 58-61 60-63 62-66 64-67

100

60 50 35 25 20

.20" .19" .18" .16" .13" .12"

Proper blower selection can be determined from these exhaust requirements and the total system static pressure loss. For outside exhaust, the enclosure must be connected to either a dedicated blower or a house exhaust system.

Labconco offers accessory remote blowers listed in Chapter 7. Contact Labconco for blower sizing assistance.

If the enclosure is connected directly to a house exhaust system, an adjustable damper (or valve) must be installed to control the airflow properly. This is equally important when a house exhaust system is controlling multiple filtered enclosures. See Chapter 7 for accessory adjustable damper ordering information.

Electrical Requirements

Standard duplex electrical receptacles should be nearby for connecting the filtered enclosure, or other equipment. The enclosures include iris pass-throughs to allow electrical cords through the back of the enclosure without leaving a large hole for contaminants to escape.

Space Requirements

The dimensions for the different models are shown in Appendix B: Dimensions.

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CHAPTER 3

GETTING STARTED

Now that the site for your filtered enclosure is properly prepared, you are ready to unpack, inspect, install, and validate your system. Read this chapter to learn how to:

 Unpack and move the enclosure.  Set up the enclosure with the proper supporting structure and work surface.  Connect to an exhaust system if applicable.  Installation of HEPA and accessory Odor Control carbon filters.  Connect the electrical supply.  Set the face velocity with the speed control adjustment.  Arrange validation for the enclosure.  Seal the enclosure to the work surface.

Depending upon which model you are installing, you may need common mechanical and electrical installation tools in addition to 5/16", 3/8", 7/16", and 1/2" wrenches, ratchets, sockets, a nut driver set, a flat-blade screwdriver, a Phillips screwdriver, and a carpenter level to complete the instructions in the chapter.

Each enclosure model weighs between 125 to 195 lbs. each (55 to 85 kg). The shipping container allows for lifting with a mechanical lift truck or floor jack. If you must lift the

enclosure manually, follow safe-lifting guidelines. Do not lift

by the front air foil.

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Chapter 3: Getting Started



The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Unpacking the Enclosure

Carefully remove the shrink-wrap or carton on the enclosure and inspect it for damage that may have occurred in transit. If damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier.

DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO. UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED.

IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER. LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES.

Do not discard the packing material until you have checked all of the components and tested the enclosure. We recommend that you do not remove the enclosure from its shipping container until it is ready to be placed into its final location. Move the unit by placing a flat, low dolly under the shipping skid, or by using a floor jack.

Do not move the enclosure by tilting it onto a hand truck.

Installing the Filtered Enclosure on a Supporting Structure and Work Surface

Use caution when lifting or moving the enclosure. When installing the enclosure onto a chemical-resistant work surface or benchtop, ensure that the structure can safely support the combined weight of the enclosure and any related equipment. The work surface should be at least as wide as the enclosure to properly support it. The front of the enclosure should be aligned within 0.36" of the front of the work surface. Mounting holes are provided in the Labconco accessory work surfaces to secure the enclosure.

Work Surface Specifications

The work surface should be smooth, rigid, and durable, such as a chemicalresistant epoxy resin. The surface should be non-porous and resistant to the biohazards, powders, solvents and chemicals used in conjunction with the Purifier Filtered Enclosure. The work surface should also contain a dished recessed area for containing primary spills.

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Chapter 3: Getting Started

Work Surface and HEPA Filtered Enclosure Installation

1. Level the base cabinets and the work surface. Work surface should be

placed flush with the front of the base cabinet as shown in Figure 3-1.

2. Position the work surface in its intended location and with the front of the

work surface towards you. (Rear mounting holes are located close to the rear edge.)

3. Secure the work surface to the base cabinet with a structural adhesive or

silicone sealant.

4. Insert the supplied mounting screws in the four holes. Allow a minimum

of 1/8" clearance under the head of the screw for positioning the enclosure.

5. Place the enclosure on the work surface and slide the rear flange and front

air foil flanges under the mounting screw heads.

6. Tighten the four screws to complete the installation.

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Filtered Enclosure Installation

Chapter 3: Getting Started

Figure 3-1

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Chapter 3: Getting Started

Connecting to the Exhaust System (Optional)

ATTENTION: The weight of the exhaust ductwork system must be supported independently of the enclosure superstructure or damage may occur.

The exhaust system should be installed by a qualified HVAC contractor.

Thimble exhaust connection aids in the removal of chemicals or applications where a higher degree of biohazard and particulate removal is required. See Chapter 7 for ordering thimble exhaust for the Purifier HEPA Filtered Enclosure or Purifier Class I Enclosure. Both 6" for house exhaust and 5" for connection to a FilterMate Portable Exhauster are available. Review Chapter 2 for exhaust prerequisites and review Chapter 7 for ordering blower exhaust equipment. See figure 3-2 and figure B-2 for thimble exhaust on Purifier filtered enclosures.

Consult Labconco Customer Service should you require help sizing your blower for the exhaust volume and system static pressure loss.

To ensure compatibility, the selected exhaust duct material should match the enclosure, procedures and chemical applications.

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Chapter 3: Getting Started

Figure 3-2

Thimble Exhaust Connection shown installed on

Purifier Models (Optional)

Part #3924400 (2'), 3924401 (3'), and 3924402 (4')

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Chapter 3: Getting Started

HEPA

ACCESSORY

CARBON

Installation of HEPA Filters and Accessory Odor Control Carbon Filters

HEPA Filters

HEPA filters are shipped installed with the gasket on the downstream side. The HEPA filters are leak checked at Labconco. A second leak check should be performed before using the enclosure and at least annually thereafter. Consult your Safety Officer and Chapter 6 for the HEPA Filter Leak Test. See Figure 4-2 for HEPA filter location, HEPA filter gasket, and filter clamp bolts. The Purifier enclosures require decontamination of biohazardous materials or are used with non-biohazardous materials or nuisance powders. All seams upstream of the HEPA filter are jacketed by negative pressure. This intrinsically safe design ensures containment.

Carbon Filters (Accessory for Odor Control only)

For carbon filter installation, first remove the upper perforated cover, secured by Phillips head screws, on the top of the enclosure. See Figures 4-1 and 4-2 for location of the upper perforated cover. See Chapter 7 for a list of available carbon filters. All carbon filters are simply gravity installed with the gasket side down and down stream on the exhaust side of the HEPA filter. Install the correct carbon filter for the application.

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Chapter 3: Getting Started

Filter Type

Appropriate Use

HEPA 2' 3707900 3' 3707901 4' 3707902

HEPA filters are high-efficiency particulate air filters having a particulate removable efficiency of 99.99% for particles with a diameter of 0.3 micron.

Organic Vapor

Activated Carbon

2' 3937200 3' 3937300 4' 3937400

Adsorbs organic compounds designated by NIOSH guidelines as acceptable for use with chemical cartridge-type respirators. Concentrations in the

enclosure’s work area must not exceed the IDLH for

the chemical and the exhaust from the enclosure must not exceed the TWA.

Formaldehyde

(Formalin) 2' 3937201 3' 3937301 4' 3937401

Formaldehyde requires the use of an impregnated carbon for the treatment of formaldehyde.

Concentrations in the enclosure’s work area must not

exceed the IDLH for the chemical and the exhaust from the enclosure must not exceed the TWA.

Ammonia and

Amines 2' 3937202 3' 3937302 4' 3937402

Treats ammonia, low molecular weight amines and other bases designated by NIOSH as acceptable for use with ammonia cartridge type respirators. Requires the use of an impregnated carbon.

Concentrations in the enclosure’s work area must not

exceed the IDLH for the chemical and the exhaust from the enclosure must not exceed the TWA.

CARBON

HEPA

CARBON

Important: Carbon filters do not provide any particulate protection, but provide odor control.

Connecting the Electrical Supply Source to the Filtered Enclosure

115V Models

Simply connect the 115V power cord supplied to the IEC electrical supply plug on the back of the enclosure. If using at 50 Hz operations, blower performance maximum airflow will be reduced by 17%.

230V Models

The above procedure applies for the 230V except it is shipped without a plug. Install the appropriate plug for your electrical specifications per local codes.

Do not use any detachable power cord that is not adequately rated

for the unit.

Ne pas utliser un fil électrique amovible qui n’est pas du tension

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nominale de l’appareil.

Chapter 3: Getting Started

Set the Face Velocity with the Speed Control Adjustment

Adjustment of the speed control gives the correct face velocity and is located behind the front panel. The face velocity should be from 75 to 105 fpm for biohazardous operations. (Consult your Safety Officer for airflow recommendations for your application). Containment is maximized at a setting within this range. Working at the lowest face velocity appropriate for the application will give the quietest operation. Face velocity measurements are made using an anemometer. An electric anemometer can be obtained from your laboratory supply dealer. Face velocity measurements should be taken in accordance with the Industrial Ventilation Manual. Using a small Phillips screwdriver, adjust the speed control to give the required face velocity. See Figures 6-1 and 6-2 in Chapter 6 to locate the speed control. The face velocity is increased by turning the speed control counter clockwise and clockwise to decrease face velocity.

Validating the Vented Enclosure

To determine the actual face velocity at the sash opening, airflow velocity readings are taken. This should be done across the sash opening of the enclosure in accordance with the Industrial Ventilation Manual. (See Appendix E) The

“average face velocity” is achieved by taking readings in two rows across the

enclosure with the readings 6" from the ends and evenly spaced every 12"; the first row is 3" down from the upper sash foil and the second row is 3" up from the work surface. A total of four readings will be taken for the 2' enclosure, six readings taken for the 3' enclosure and eight readings taken for the 4' enclosure and then averaged. Refer to Chapter 2 for proper airflow volumes for your particular model.

The Purifier enclosures have been tested at Labconco’s airflow test facility per ASHRAE 110-1995. All enclosures achieve an “as manufactured rating” of less than 0.05 part per million (ppm) at 4 liters per minute (lpm); AM <0.05 (Consult

Labconco for individual ratings). For “field use” ASHRAE testing contact

Labconco for a certified on-site contractor. Labconco also performed extensive ASHRAE testing to validate the enclosures for gas containment. For copies of these validation reports, contact Labconco Customer Service.

The Purifier Enclosures were also subjected to Biological Containment testing, using a modified Personnel Protection Test, as described in NSF/ANSI Standard Number 49. An aerosol challenge of approximately 5 x 108 spores of Bacillus subtilus var Niger were released in the enclosure and appropriate air sampling equipment established the number of spores that escaped through the sash opening. Each size enclosure was tested three times. All three sizes of the enclosure were tested at 75 FPM inflow and typically 1 or less than one spore escaped from the enclosure during each of the tests.

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Chapter 3: Getting Started

The Purifier Enclosures were also subjected to an industrial hygiene air-monitoring study to confirm the performance of the enclosure for powders. The Purifier Enclosures demonstrated excellent containment when used by an operator using excellent technique and good containment when used by an operator using marginal technique. While no enclosure can compensate for improper technique, these tests confirm the Purifier Enclosures provide a safe working environment.

NOTE: Face velocity profiles and smoke testing should be

performed frequently per your organization’s quality system to

ensure safe performance.

Sealing the Filtered Enclosure to the Work Surface

When the filtered enclosure has been set in place, ducted, (if necessary), and wired, it may be sealed at the work surface to prevent spilled materials from collecting under the walls. A bead of silicone sealant is recommended to seal the filtered enclosure to the work surface.

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CHAPTER 4 HIGH PERFORMANCE FEATURES AND SAFETY PRECAUTIONS

High Performance Features

The patented1 Purifier HEPA Filtered Enclosure and Purifier Class I Enclosure are designed to meet the needs of the laboratory scientist, and provide superior containment while operating at velocities between 75-105 feet per minute. The filtered enclosures have been tested to effectively contain toxic, noxious, and biohazardous materials when properly installed and operated. What makes the filtered enclosures so unique is the revolutionary way they direct air into and through the contaminated air chamber. Labconco engineered the filtered enclosures to minimize the effects of turbulence. The containment-enhancing and aerodynamic designs of the upper sash foil, side air foils, lower air foil, upper dilution air supply, and rear perforated baffle all work in concert to produce horizontal airflow patterns that significantly reduce powder, chemical and biological material concentrations through the work area.

These concentrations of materials are predominantly removed on the “first pass” of

airflow through the chamber resulting in high performance containment. The plenum and the HEPA filter are jacketed by negative pressure. Should a leak occur in the filter gasket or the plenum, the contaminated air is recaptured and refiltered.

The Purifier Class I Enclosure and Purifier HEPA Filtered Enclosure have an access port located behind the front panel. For HEPA filter removal in biohazardous applications on Purifier enclosures, the HEPA filter will require decontamination before removal. Users of all equipment are encouraged to routinely check airflow or purchase a model with an airflow monitor.

U.S. Patent No. 6,461,233

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Chapter 4: High Performance Features and Safety Precautions

3 8 18

29 5 6

Not Shown 7, 9, 10, 11, 12, 13, 20, 21, 22, 23, 24, 25, 28 (See Figure 4-2)

Figure 4-1

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Chapter 4: High Performance Features and Safety Precautions

HEPA Filtered Enclosure Airflow Diagram

Figure 4-2

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Chapter 4: High Performance Features and Safety Precautions

Figure 4-3

Figure 4-4

1. Aerodynamic Clean-Sweep™ Air Foil has a unique shape that allows air

to sweep the work surface for maximum containment. The Clean-Sweep™ openings create a constant protective barrier from contaminants. Should the operator inadvertently block the airflow entering the air foil, air continues to pass under the air foil and through the Clean-Sweep openings. See Figures 4-1 and 4-3.

2. Containment-Enhancing Upper Sash Foil includes an open air passage

directly atop the sash foil to bleed air into the hood chamber and direct chemical, powder and particulate materials away from the sash opening. The radiused sash foil sweeps airflow into the hood with minimal turbulence. See Figures 4-1 and 4-4.

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Chapter 4: High Performance Features and Safety Precautions

3. Upper Dilution Air Supply provides bypass air from above the work area.

This feature constantly bathes the inside of the sash with clean air and reduces powders, particulate materials and chemical fumes along the sash plane, near the critical breathing zone. Five to seven percent of the required air volume is introduced through the upper dilution air supply. The upper dilution air supply also reduces stagnant pockets of air in the upper interior. See Figure 4-2.

4. Zoned Rear Perforated Baffle directs horizontal laminar air streams to

the three-zoned sections of the perforated baffle. The three-zoned sections have increasingly more open area at the bottom that help form laminar airflow. This minimizes the potential for air to roll forward preventing contaminants from moving toward the sash opening. The majority of contaminants are highly diluted, captured and removed on the first pass through the enclosure. See Figures 4-1 and 4-2.

5. Side-Entry Air Foils allow turbulence-free air to enter the enclosure from

the sides and allow clean air to sweep the interior walls of the enclosure. See Figures 4-1 and 4-2.

6. Ergonomic Slope of 10 degrees provides maximum visibility, and comfort

reduces glare, thereby minimizing operator fatigue. See Figure 4-1.

7. Internal Depth of 23" provides necessary depth to support modern

balances and other auxiliary equipment without extending outside the enclosure or resting on the lower air foil. See Figure 4-1

8. Safety Glass Sash with Spring-Loaded Latch has a wiping seal to

contain contaminants and features a spring-loaded latch to secure sash open for loading and cleaning. The sash must be down for normal operation. See Figure 4-1.

9. Utility Ports with Iris allows electrical cords and data cords to pass

through the back of enclosure without leaving a large hole for contaminants to escape. The enclosure ships with solid plugs. Iris plugs are included with the User’s Manual. See Figure 4-1 and 4-2.

10. Accessory Guardian™ Airflow Monitor continuously monitors airflow.

An audio/visual alarm alerts the user to low airflow conditions. The Guardian™ Airflow Monitor is an available option on all Purifier models. See Figure 4-1 and 4-2.

11. Inherently Safe Impeller has a negative pressure plenum that surrounds

the positive pressure impeller so that if a leak should occur, the unfiltered air is captured and refiltered. See Figure 4-2.

12. Vibration-Isolated Motorized Impeller has vibration isolation supports,

which eliminates transfer of vibration to the work surface. This is extremely useful in weighing operations with precision balances. The impeller wheel is also dynamically balanced. See Figure 4-2.

13. Speed Control regulates the speed of the motorized impeller and is used

by the certifier to validate and adjust the inflow velocity. Located behind the front panel. See Figure 4-2.

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14. Space-Saving Design increases effective laboratory workspace, because

the impeller and HEPA filter are contained within the enclosure, a separate filtered blower module is not required. See Figure 4-1

15. Fluorescent Lamp is located above the work area, out of contact with

contaminated air. A safety glass window beneath the lamp distributes the light evenly across the work surface. See Figure 4-1.

16. Filter System Pressure Gauge indicates the total system pressure across

the HEPA filter and pressurized plenum to help predict HEPA filter loading. See Figure 4-1.

17. Filter Clamping Bolt evenly seals the HEPA filter to the frame of the

enclosure. See Figure 4-1 and 4-2.

18. Sash. The sash is constructed of 1/4 inch tempered safety glass. It pivots

up for loading and cleaning. See Figure 4-1

19. Control Panel. The control panel, which is located above the sash,

contains the filter system pressure gauge, control switches and the electronics. See Figure 4-1.

20. HEPA (High Efficiency Particulate Air) Filter is rated to remove

99.99% of all particles 0.3 micron in size. See Figure 4-2.

21. Lamp Ballast (Not Shown) for the fluorescent lamp is located behind the

control panel.

22. UV (Ultraviolet) Lamp (Not Shown), found only on Purifier Class I

Enclosure, allows the operator to surface disinfect the work area of the enclosure when it is not in use. The UV will not penetrate the plane of the sash and lower airfoil (see item 29).

23. Accessory Work Surface (Not Shown) is dished and contoured to fit the

dimensions of the enclosures to contain spills.

24. Accessory Odor-Control Carbon Filters are available for nuisance odor

control. Accessory carbon filters are designed to remove trace amounts of noxious fumes; capacity is not adequate to remove continuous fume generation. The carbon filters are supported above the HEPA filter and the gasket side is down and compressed by the weight of the carbon filter. Carbon filters are listed in Chapter 7. See Figure 4-2.

25. Accessory Thimble Exhaust Connections are available to duct the

Purifier Class I Enclosure and Purifier HEPA Filtered Enclosure to the outside. The Thimble Exhaust Connection allows the operator additional protection from hazardous fumes or vapors and should be used if the carbon filter capacity is not adequate. Each Thimble Exhaust Connection includes an epoxy-coated steel exhaust transition adapter designed for 6" diameter nominal duct. See Figure 3-2 in Chapter 3 and figure B-2 in appendix B.

26. Upper Diffuser Screen provides protection for the HEPA Filter and (LED)

airflow sensor. See Figure 4-1 and 4-2.

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27. HEPA Filter Access Port to access HEPA filter during filter changing

procedures. For harmful powders and particulates, the true bag-in/bag-out procedure is used to properly dispose of the contaminated HEPA filter. See Figure 4-2.

28. Utility Shelves (Not Shown) allows the user to hold items on shelves

inside the enclosure. See Chapter 7.

29. UV Closure Panel provides protection when the UV lamp is on. The panel

must be in place for the UV lamp to work.

Safety Precautions

1. Although the enclosure has been engineered to maintain optimum operator

safety, caution should always be used while working. Prior to using the enclosure, check to make sure that the exhaust blower is operating and that air is entering the enclosure at its specified face velocity. The use of an airflow monitor is recommended to alert the user to a problem with airflow.

2. Use good housekeeping in the enclosure at all times. Clean up spills

immediately. Periodically clean enclosure interior.

3. Do not overload the work surface with apparatus or work material. The

safe operation of the enclosure is based upon having proper airflow through the structure. Do not place large objects directly on the work surface. Instead, elevate the object 3/4" on blocks to allow a flow of air under the object and into the rear baffle exhaust slots. Ensure blocks are level and secured in place.

4. Blocking large portions of the rear baffle will change the airflow pattern in

the enclosure causing turbulence. (Do not store containers or supplies against the rear baffle, as this will affect airflow).

5. Always work with your hands as far back into the enclosure as possible.

Keep all biohazardous materials, chemicals and apparatus inside the lower air foil of the enclosure.

6. Do not work in this enclosure without the exhaust system running.

7. Perchloric acid use in this enclosure is prohibited.

8. High-level radioisotope materials are prohibited in this enclosure. Consult

your Safety Officer.

9. Avoid cross drafts and limit traffic in front of the enclosure. Air

disturbances created may draw contaminants out of the enclosure.

10. A qualified certification technician should test the enclosure before it is

initially used. The enclosure should be validated whenever it is relocated.

11. The use of safety goggles, protective clothing, gloves and any other

personal protective equipment recommended by your safety officer should be used.

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Chapter 4: High Performance Features and Safety Precautions

12. The sash must remain in the down position while using the enclosure.

13. Proper performance of the enclosure depends largely upon its location and

the operator’s work habits. Consult the references in Appendix D.

14. The enclosure should be recertified whenever it is serviced or at least

annually thereafter.

15. The HEPA filter provides personnel and environmental protection from

particulate matter. Because room air is drawn over the work surface during operation, this enclosure should not be used for operations requiring product protection from environmental contamination.

16. Avoid the use of flammable gases or solvents in the enclosure if possible.

Care must be taken to ensure against the concentration of flammable or explosive gases or vapors. Use of an open flame should be avoided in the enclosure. Open flames may disrupt the airflow patterns in the cabinet,

burn the HEPA filter and damage the filter’s adhesive. Gases under high

pressure should not be used in the enclosure as they may disrupt the airflow patterns of the cabinet.

17. HEPA filters are only effective for entrapment of particulate matter.

Manipulations that generate gases or vapors from toxic chemicals or radionuclides, must be evaluated carefully from the standpoint of buildup to dangerous levels, the decontamination of the enclosure and compliance with applicable regulations.

18. The surface of the HEPA filter is fragile and should not be touched. Care

must be taken to avoid puncturing the HEPA filter during installation or normal operation. If you suspect that a HEPA filter has been damaged DO NOT use the enclosure; contact a local certification agency or Labconco.

19. The HEPA filter in the enclosure will gradually accumulate airborne

particulate matter from the room and from work performed in the enclosure. The rate of accumulation will depend upon the cleanliness of the room air, the amount of time the enclosure is operating and the nature of work being done in the enclosure. With normal usage, the HEPA filters will last two to five years before requiring replacement.

20. Avoid direct exposure to ultraviolet (UV) radiation; NEVER work in the

Purifier Class I Enclosure when the UV light is on. Verify the sash is closed and the UV closure panel is in place before turning on the UV light

21. Ensure that the filtered enclosure is connected to an electrical service in

accordance with local and national electrical codes. Failure to do so may create a fire or electrical hazard. Do not remove or service any electrical components without first disconnecting the filtered enclosure from electrical service.

22. Use of an open flame must be avoided with the enclosure. Open flames

may disrupt the airflow patterns in the enclosure and cause a fire hazard with volatile solvents.

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23. Increase the face velocity by adjusting the speed control of the motorized

impeller or change the HEPA filter when the face velocity falls below acceptable limits established by your Safety Officer.

24. Exhaust carbon filters and HEPA filters are disposed as hazardous waste.

The user is responsible for recording the chemicals adsorbed or treated by the filters and disposing properly. HEPA filters should be decontaminated before disposal on Purifier enclosures.

25. Ensure only trained operators use the enclosure. New users should review

the User’s Manual and become familiar with the operation of the enclosure.

26. If the filtered enclosure is to be used in a confined space, make sure the

space is well ventilated and the concentration of toxic contaminants cannot accumulate greater than the TWA.

27. Proper operation of the enclosure depends largely upon the enclosure

location and the operator’s work habits. The enclosure should be located

away from traffic patterns, doors, fans, ventilation registers, fume hoods, and any other air-handling device that could disrupt its airflow patterns. Consult Chapter 2: Prerequisites and Chapter 3: Getting Started sections of this manual for further details.

28. Only nuisance powders, biohazardous and particulates removed by HEPA

filters and trace chemicals which can be safely adsorbed and treated with specific carbon based filters are appropriate for use in this enclosure.

29. The warning properties (i.e., odor, taste) of the volatile organic compounds

or other material being used in the enclosure must be adequate to provide an early indication that the carbon filter may be saturated or inadequate. Contact Labconco for help with chemical assessment.

30. Use the smallest possible quantity of chemical(s) within the enclosure and

never exceed the amount, which can be effectively adsorbed by the Odor Control carbon filter before breakthrough.

31. Adjust face velocity to fall between 75 and 105 FPM for biological

procedures.

32. Leave the blower on for at least one minute after work in the enclosure has

been completed.

33. If a chemical, powder or particulates are spilled on the work surface, DO

NOT switch off the blower until all traces have been removed.

34. Tag enclosures with appropriate warning, if filters have been removed for

service.

35. If the blower fails during use, processes should cease and the area should

be vacated and ventilated.

36. Always refer to the NIOSH Pocket Guide to Chemical Hazards before

proceeding. For additional help with filter and chemical selection contact Labconco at 1-800-821-5525 or 1-816-333-8811.

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CHAPTER 5

APPROPRIATE APPLICATIONS

FOR YOUR FILTERED

ENCLOSURE

Now that the installation of your filtered enclosure is completed, you are ready to use your filtered enclosure. Read this chapter to learn about:

1. Routine Daily Work Procedures.

2. Suitable Applications.

3. Appropriate HEPA Filter Applications, Suitability and Guidelines.

4. Odor Control Carbon Filter Applications.

5. Definition of Terms.

6. Appropriate Chemicals for Odor Control Carbon Filters.

7. Hazardous Misapplications for Odor Control Carbon Filters.

8. Chemical Carcinogen use with Odor Control Carbon Filters.

9. Prohibited Acid Use.

Routine Daily Work Procedures

Planning

 Thoroughly understand procedures and equipment required before

beginning work.

 Arrange for minimal disruptions, such as room traffic or entry into the

room while the enclosure is in use.

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Chapter 5: Appropriate Applications for Your Filtered Enclosure

Start-up

 Turn on exhaust system and accessory light. Turn off the UV light, if

equipped.

 Only raise the sash for loading and cleaning.  Check the baffle air slots for obstructions.  Allow the enclosure to operate unobstructed for 1 minute.  Wear a long sleeved lab coat and rubber gloves. Use protective eyewear.

Wear a protective mask if appropriate. Consult your Safety Officer for additional personal protective equipment recommendations.

Loading Materials and Equipment

 Load only the materials required for the procedure. Do not overload the

enclosure.

 Do not obstruct the air foil, or rear baffle slots.  Large objects should not be placed close together and should be elevated

above the work surface to permit airflow to sweep under the equipment.

 After loading, wait one minute to purge airborne contaminants from the

work area.

Work Techniques

 Keep all materials inside the lower air foil, and perform all contaminated

operations as far to the rear of the work area as possible.

 Segregate all clean and contaminated materials in the work area.  Avoid using techniques or procedures that disrupt the airflow patterns of

the enclosure.

Final Purging

 Upon completion of work, the enclosure should be allowed to operate for

two to three minutes undisturbed, to purge airborne contaminants from the work area before shutting down the blower.

 If equipped, install the UV closure panel and turn on the UV light to

decontaminate the work area as determined by your Safety Officer.

Unloading Materials and Equipment

 Objects in contact with contaminated material should be surface

decontaminated before removal from the enclosure.

 All open trays, weigh vessels or containers should be covered before being

removed from the enclosure.

Shutdown

 Turn off the exhaust system and light.  Turn off the UV light, if applicable.

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Chapter 5: Appropriate Applications for Your Filtered Enclosure

Suitable Applications

NIOSH (National Institute for Occupational Safety and Health, USA) has established guidelines for chemical cartridge and HEPA filtered respirators. Suitable applications for the filtered enclosures are based on these guidelines. As with respirators, chemical contaminants are adsorbed or treated by carbon; particulates, powders and biohazardous materials are filtered by the HEPA filter before air is returned to the room. Biohazardous materials or other hazardous solids can be used with the HEPA filtered enclosure as long as appropriate decontamination occurs before filter changing operations. See Chapter 6 for decontamination procedures.

Listed below are suitable applications for HEPA filters and accessory Odor Control Carbon Filters.

HEPA Filter Applications, Suitability and Guidelines

 Biohazardous materials and hazardous solids to protect only the operator and

the surrounding environment.

 Weighing of powders.  Release of particulates using a HEPA filter.  Powder or particulate procedures traditionally performed on an open bench.  The HEPA filtered enclosure provides personal and environmental protection

from particulate matter, hazardous powders and biohazardous materials. Because room air is drawn through the enclosure during operation, this enclosure should not be used for operations requiring sample protection from environmental contamination.

 HEPA filters are only effective for entrapment of particulate matter, hazardous

powders and biohazardous materials. Manipulations that generate gases or vapors, i.e., toxic chemicals or radionuclides, require the use of a HEPA and carbon filter in combination. Otherwise the enclosure should be ducted to the outside. The surface of the HEPA filter is fragile and should not be touched. Care must be taken to avoid puncturing the HEPA filter during installation. If you suspect that a HEPA filter has been damaged, DO NOT use the enclosure. See Chapter 6 for Replacing the HEPA Filter.

 The HEPA filter will gradually accumulate airborne particulate matter from

the room and from work performed in the enclosure. The rate of accumulation will depend upon the cleanliness of the room air, the amount of time the enclosure is operating, and the nature of work being done. With normal usage, the HEPA filters will last two to five years before requiring replacement.

 Each filtered enclosure is provided with a HEPA filter. For biohazardous

materials, follow the Decontamination Procedure in Chapter 6 for disposal instructions.

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Chapter 5: Appropriate Applications for Your Filtered Enclosure



Odor Control Carbon Filter Applications (sold as an accessory)

 Release of low concentrations of vapors effectively adsorbed or treated in

carbon based filters.

 Treatment of low-level carcinogens or suspected carcinogens. (See Chemical

Carcinogens in Chapter 5)

 Procedures that may have traditionally been done on the open bench (low

levels only).

 Odoriferous chemicals that are an unpleasant nuisance.

Other applications, not fitting the above guidelines, would be better suited with the exhaust ducted to the outside. The exhaust connection kit (See Chapter 3 and Chapter 7) can be added to the filtered enclosures for ducting to the outside. Labconco also manufactures fume hoods and Class II Biological Safety Cabinets for exhausting to the outside. Consult Labconco product specialist for additional information.

REFERENCES TO NIOSH OR OSHA GUIDELINES AND REGULATIONS APPLY TO ANY WORK PLACE UNDER THE JURISDICTION OF THE U.S. DEPARTMENT OF LABOR. OTHER COUNTRIES OUTSIDE THE U.S. HAVE ESTABLISHED STANDARDS, WHICH MAY DIFFER SLIGHTLY FROM THOSE USED AS GUIDELINES FOR THIS

PRODUCT. IT IS THE USER’S RESPONSIBILITY TO

BECOME AWARE OF LOCAL REGULATIONS GOVERNING THE SAFE USE AND DISPOSAL OF CHEMICALS, CARBON AND HEPA FILTERS. KNOWLEDGE OF ESTABLISHED SAFE EXPOSURE LEVELS IS IMPERATIVE TO THE PROPER USE OF FILTERED ENCLOSURES.

Definition of Terms

NIOSH – National Institute for Occupational Safety and Health/Mine Safety and Health Administration. (U.S.A.)

TWA – Recommended Exposure Limits expressed as a Time Weighted Average. The exposure limit for that chemical for up to a 10-hour workday, 40 hours a week. Expressed in units of parts per million or milligrams per cubic meter.

Odor Threshold – The value in parts per million or milligrams per cubic meter for which one might expect to smell a chemical’s presence in the air. This value is

very subjective and detection will vary with the sensitivity of one’s nose. The

period of time until the odor threshold is reached in the exhaust stream can be estimated from Labconco exclusive computerized filter modeling program. Contact Labconco on carbon filter life for specific applications. See Chapter 6.

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Chapter 5: Appropriate Applications for Your Filtered Enclosure

Saturation Level or Time – There is a limit to the amount of chemical that can be adsorbed by activated carbon, or neutralized by chemically-treated carbon. Once the capacity of the carbon is reached, it is considered to be saturated and will adsorb (or neutralize) no further material; the outlet concentration of the chemical will equal the inlet concentration from that point until the filter is replaced. (Note that the capacity of activated carbon is not a constant, but varies with the inlet concentration). Labconco Technical specialists can determine with the computerized carbon-modeling program the estimated time saturation for a particular chemical. When using a HEPA filter alone in all filtered enclosures or in combination with an Odor Control carbon filter, the speed control will need to be increased to allow for HEPA filter loading. Measure filtered enclosure face velocity with an anemometer.

IDLH (Immediately Dangerous to Life and Health) – An atmosphere that poses an immediate hazard to life or produces immediate irreversible health effects. IDLH concentrations should not be approached in the enclosure.

Appropriate Chemicals for Odor Control Carbon Filters

Below is a general set of rules to determine appropriateness of chemical usage.

Selected organic chemicals considered to be occupational carcinogens by NIOSH can be used in the filtered enclosure with carbon filters under rigid restrictions. See separate discussion on carcinogens for special instructions.

Organics must have time weighted exposure limits (TWA) of 1 PPM or greater. Chemicals must have a detectable odor at concentrations below the TWA for the

chemical. Chemicals must be designated by NIOSH guidelines as acceptable for use with

chemical cartridge-type respirators (the exception is formaldehyde and ammonia/amines, which used impregnated carbon). Chemicals not listed by NIOSH in the Pocket Guide must be approved by Labconco Product Specialist (or Engineering).

Inlet concentration must never exceed the IDLH (Immediately Dangerous to Life and Health) concentrations.

Chemicals having a recommendation by NIOSH of at least “Escape GMFOV”

(Gas Mask Full-Face Respirator). When evaporating a mixture of chemicals, the chemical having the lowest TWA

will be used to determine if the mixture meets the guidelines. Call a Labconco Product Specialist at 1-800-821-5525 for assistance in chemical

appropriateness.

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Chapter 5: Appropriate Applications for Your Filtered Enclosure

Hazardous Misapplications for Odor Control Carbon Filters with Volatile Chemicals

There is one scenario where the accessory carbon filter misapplication would be a part of a hazardous condition. If the user continues to operate the enclosure with any of the following conditions present a potentially hazardous condition will exist:

1. The inlet concentration of vapors is greater than the TWA.

2. The carbon filter becomes saturated.

3. The ventilation of the room is insufficient to dilute the exhaust of the

enclosure to below the TWA for the chemical.

When the inlet concentration is greater than the TWA, extra measures must be taken to monitor the filter and number of room air exchanges.

Chemical Carcinogen Use with Odor Control Carbon Filters

Selected carcinogens may be used safely with Odor Control carbon filters under the following restrictions.

The use of a vented fume hood or ventilated enclosure with ducting to the outside is always the preferred method when working with carcinogens. The Odor Control carbon filters should only be used, as a last resort when venting to the outside is not an option.

The potential carcinogens are listed in the NIOSH Pocket Guide to Chemical

Hazards as “Ca.” Each potential carcinogen must have a TWA of 1 or greater;

have minimum respirator recommendation of Escape GMFOV, and an odor threshold significantly lower than the TWA for the chemical. The inlet concentration or the evaporation rate of the chemical must never exceed the TWA for the chemical.

Consult a Labconco Technical Specialist for estimated saturation life. See Chapter 6 for an example of estimating saturation life. Another source is the Labconco chemical guide for carbon filtered enclosures.

Prohibited Acid Use

The Purifier HEPA and Purifier Class I filtered enclosures motorized impeller cannot be exposed to acids. Where applications require the use of acids, a separate ventilated enclosure or vented fume hood should be used with a remote blower ducted to the outside. No exceptions are permitted, as the impeller life span will be limited with acid use.

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CHAPTER 6 MAINTAINING YOUR FILTERED ENCLOSURE

Monitoring airflow and changing the filters is the primary maintenance required. Decontamination may be required and is reviewed in Chapter 6. Certification and recertification is reviewed in Chapter 6.

Review this chapter on maintenance for the following:

1. Routine Maintenance.

2. Decontamination.

3. Determination of when to replace the HEPA filters.

4. How to install a new HEPA filter.

5. HEPA filter leak test.

6. Speed control adjustment and setting the inflow face velocity.

7. Operating and calibrating the airflow monitors.

8. Determination of when to replace Odor Control carbon filters and how to

replace.

9. Calculating Odor Control carbon filter life.

10. Initial certification.

11. Re-certification.

12. Fluorescent light replacement.

13. UV light replacement on Purifier Class I.

14. Motorized impeller replacement.

15. Speed control replacement.

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Chapter 6: Maintaining Your Filtered Enclosure

HEPA

CARBON

Routine Maintenance Schedule

Weekly

 Wipe down the interior surfaces of the enclosure with a disinfectant or

cleaner, depending upon the usage of the unit and allow to dry.

 Using a damp cloth, clean the exterior surfaces of the enclosure,

particularly the front and top to remove any accumulated dust.

 Operate the exhaust system, noting the airflow velocity through the

enclosure using a source of visible smoke. Airflow monitors are recommended for constant monitoring.

Monthly (or more often as required)

 Determine the actual face velocity through the sash opening of the

enclosure where the average reading should be at the specified velocity. (Use calibrated thermal anemometer or other approved apparatus). Airflow alarms are recommended for constant monitoring.

 The enclosure rear baffle should be checked for any blockage to ensure that

the enclosure is maintaining proper airflow.

 All weekly activities.  Check face velocity. Increase speed control or change HEPA filter when

face velocity of the enclosure drops below the recommended speed for your facility or if the airflow alarm monitor alerts you. Airflow monitors are recommended.

 While the enclosure is filled with the contaminant, test filter condition on

Odor Control carbon filters using the appropriate gas detector tube at intervals of 20% of the total estimated time. The exception to the 20% recommendation is formaldehyde or any carcinogen or suspected carcinogen. These hazardous chemicals must be checked at least every 10% of the total estimated time. Gas detector tubes for the specific chemicals that are being used in the enclosure can be obtained from your laboratory supply dealer.

 Replace Odor Control carbon filters when chemical breakthrough is

indicated by odor, time, detector tube, or for some chemicals, analytical

instrumentation. See “Replacing Odor Control Carbon Filters” section of

this manual in Chapter 6.

Annually

 Replace the fluorescent lamps. Replace UV lamps if equipped.  Verify proper operation of the UV closure panel safety switch.  Have the enclosure validated by a qualified certification technician. See

Certification and Recertification in Chapter 6.

 All monthly activities.

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Chapter 6: Maintaining Your Filtered Enclosure

Decontamination

When used in conjunction with biohazards, the Purifier Class I Enclosure and Purifier HEPA Filtered Enclosure should be decontaminated with formaldehyde gas before:

 maintenance work in contaminated areas  HEPA filter changes  moving the cabinet to a new location  changing research programs  after a gross spill of biohazardous material

The procedures for performing a gaseous decontamination are thoroughly outlined in the U.S. Department of Health, Education and Welfare booklet entitled Formaldehyde Decontamination of Laminar Flow Biological Safety Cabinets, available from NIH, Division of Safety, Bethesda, MD 20892, call 301-496-2801, or from Labconco Corporation.

Determination of When to Replace HEPA Filters

The HEPA filters in the filtered enclosures gradually accumulate airborne particulate matter and powders from the enclosure and room. The rate of accumulation will depend upon the cleanliness of the room air, the amount of time the enclosure is operating, and the nature of work being done in the enclosure. In typical installations and usage, the HEPA filters will last two to five years before requiring replacement. Replace HEPA filters when face velocity drops below the recommended 75-105 fpm velocity, and the speed control is adjusted to full speed. Replace HEPA filters if it fails the HEPA Filter Leak Test in Chapter 6.

How to Install a New HEPA Filter

NOTE: The enclosure must be properly decontaminated before servicing the

HEPA filter. Only a qualified certifier should service the HEPA filter. After the HEPA filter is replaced, the enclosure MUST be certified. See Figure 6-1.

1. Unplug the enclosure.

2. Remove the front panel by loosening the two screws that secure it, and then

remove the filter access cover.

3. Using a 9/16" deep socket, loosen the filter clamp bolts located on top.

Refer to Figures 4-1 and 4-2.

4. With the clamp bolts loosened, the HEPA filter-clamping frame should be

clear of filter. Carefully pull the filter straight out of the enclosure and discard properly.

5. With the filter removed, inspect the clamping frame and the frame of the

cabinet for damage.

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Chapter 6: Maintaining Your Filtered Enclosure

ATTENTION: The filter clamp bolts should only be tightened enough to ensure a proper seal at maximum tightness. The filter gasket should be compressed 50% or less.

6. Cover the surface of the new HEPA filter gasket with a light coating of

silicone grease, if desired.

7. Install the new HEPA filter by pushing it straight into the cabinet, ensuring

that it is correctly oriented with the gasket facing up on the exhaust side of the enclosure. The filter gasket is surrounded by a negative pressure plenum and the gasket will not leak. Be sure the filter fits properly.

8. Tighten the clamp bolts uniformly until the filter gasket is properly

compressed against the frame. Inspect the seal thoroughly before proceeding.

9. Reinstall the filter access cover and the front panel.

10. Plug the enclosure in and have it recertified before use.

Do NOT contact blower wheel while still in motion.

NE PAS être en contact avec la roué du ventilateur tant qu’il est

en marche.

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Chapter 6: Maintaining Your Filtered Enclosure

HEPA Filter Changing Diagram & Filter Leak Test Diagram

Figure 6-1

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Chapter 6: Maintaining Your Filtered Enclosure

HEPA

HEPA Filter Leak Test

Purpose

After installing the new HEPA filter, the HEPA filter should be leak checked. This test is performed to determine the integrity of the HEPA filter, the filter housing, and the filter mounting frames. Leak testing is to be done by a qualified technician with calibrated equipment. Remove the top perforated exhaust cover by using a Phillips screwdriver to unfasten the (2) screws used to secure (2) clips. See Figure 6-1. The filter passes the leak test at .01% or better. Reference Leak Testing and Photometer scanning from the Institute of Environmental Services (IES-RP-CC001.3)

Apparatus

1. An aerosol photometer ATI model 2D, 2E, 2G or equivalent. Air

Techniques Hamilton Associates inc. 11403 Cron Ridge Dr. Owings Mills, MD 21117

2. One aerosol generator of the Laskin nozzle(s) type. An aerosol of mineral

oil or suitable liquid shall be created by flowing air through it. The compressed air supplied to the generator should be adjusted to a pressure of 10± 1 psig. during operation. Air Techniques Inc. Model TDA-4A or equal. One nozzle at 10 psig is (67.5 cfm x 100ug/l)/(Vol. of air), For the 2' at 90 fpm or 130 cfm, one nozzle @10 psig is 13,500/130 cfm = 52 ug/l. For the 3' at 90 fpm or 200 cfm, one nozzle @ 10 psig is 34 ug/l. For the 4' at 90 fpm or 265 cfm, one nozzle at 10 psig is 26 ug/l.

3. Mineral oil (Catalog #1491400).

4. Sampling Nozzle, Rectangular 1/2" x 3-1/4", Air Techniques, Inc.

Procedure

For the ATI 2G Photometer

1. Turn on the photometer and allow it to operate for a minimum of 5 minutes.

Leave the valve in the “CLEAR” setting.

2. Press the “ENTER” keypad. Press the “REF” keypad.

The display will display “P1” for approximately 1 second, and then display

a numerical value.

3. The display will display “P1” for approximately 1 second, and then display a

numerical value.

4. Using the “^” or “ν” keypads, respectively, increase or decrease the numerical

value until it equals 52 (2'), 34 (3'). 26 (4') for the enclosure at 90 fpm inflow velocity.

5. Press the “ENTER” Keypad. The photometer will scan for 15 seconds, and

then the “0” keypad will flash. Press the “Enter” keypad. The unit will scan for 5 seconds, the display will read “0000,” and the unit will sound a confirming

tone.

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Chapter 6: Maintaining Your Filtered Enclosure

6. Set the valve to “DOWNSTREAM.” Place the palm of your hand over the

sampling port of the pistol. There should be a strong vacuum at this port. If the vacuum is weak, contact Air Techniques Hamilton Associates.

7. Turn the enclosure on and let it operate for a minimum of 5 minutes.

8. If necessary, adjust the speed control of the enclosure to maintain the following

airflows at 90 fpm; 2' (130cfm), 3' (200 cfm, 4' (265 cfm.)

9. Position the aerosol generator discharge in the intake of the baffle inside the

enclosure.

10. Start the aerosol generator (Pressure to be +/- 1 PSIG). Ensure that one Laskin

nozzle is in the “open” position.

11. Allow the generator to operate for a minimum of 15 seconds. For all integral

motorized impeller models, scan the downstream exhaust side of the HEPA filter by passing the sampling nozzle of the gun in slightly overlapping strokes over the entire surface the filter, with the sampling port not more than 1 inch from the surface of the filter media. Scan the entire periphery of the filter and the gasket between the filter frame and the enclosure frame. Scanning shall be done at a traverse rate of not more than 2 inches per second.

NOTE: For the Purifier Enclosures ducted to the outside, place the

sampling nozzle in the center of the remote blower exhaust.

Acceptance

Aerosol penetration shall not exceed 0.01 percent measured by the photometer.

Setting the Inflow Face Velocity with the Speed Control Adjustment

1. Remove the front panel by loosening the (2) Phillips screws on top that

secure the front panel.

2. The speed control is located on the electrical subassembly located behind

the switched control panel and below the front panel. See Figure 6-1.

3. Adjust the speed control with a small Phillips screwdriver by turning the

screw counterclockwise to increase blower speed or clockwise to decrease the blower speed. The speed control is very sensitive, so proceed with caution.

4. Measure the inflow velocity per the averaging technique outlined in

Chapter 3 and adjust the speed control slowly for the desired speed. Allow the speed to stabilize and re-measure the inflow velocity to confirm.

5. Replace the front panel and tighten the screws.

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Chapter 6: Maintaining Your Filtered Enclosure

Opt ions

Calibrate and Operate the Airflow Monitor

Guardian Airflow Monitor (LED Monitor)

Refer to Figure 6-2 for operation and calibration.

Labconco Airflow Monitor / Airflow Switch Operation

The Guardian Airflow Monitor (LED) consists of a circuit board and an airflow switch. This switch indicates airflow as safe or low. It does not provide an actual face velocity, but a small setscrew in the back of the sensor can adjust the airflow level that it classifies as “good/safe” or “low/alert.”

The circuit board provides power to the sensor and also contains a “safe (green)” and “alert (red)” airflow LED indicators, as well as a “SILENCE ALARM” button

to quiet the audio alarm. When first powered up, the PCB will light both red and green LED indicators and sound the alarm to indicate it is working. After 5 seconds, the air monitor will indicate either good or bad airflow based on what the connected airflow switch detects. For low airflow, the unit will wait for 10

seconds of bad indications before it sounds both the audio alarm and the red “alert”

LED indicator. If the “SILENCE ALARM” button is pressed, the audio alarm will

be silenced, but the red “alert” LED will remain on. The alarm is silenced

indefinitely unless an airflow change is detected. If safe airflow is later detected

for 10 seconds, the green “safe” LED will be lit and the “alert” (red) LED will be

shut off. At any time the airflow is safe/good, one can press the SILENCE ALARM test button and the audio alarm and the red LED will turn on as long as this button is held down. The PCB has also a two-pin connector for use as an external output with isolated relay contacts that close when the red/alert LED is lit (low airflow). These relay contacts are not affected by the “SILENCE ALARM” button.

The PCB is mounted behind the front panel using standoffs and an appropriate label

is used to highlight the “SILENCE ALARM” button with clear areas for the red and

green LED’s. No holes to allow sound to be broadcast louder are necessary.

The PCB can be prepared as a factory special with an additional connector for the following external inputs, and having the following possible functions:

 External Alarm allows an external signal to sound the alarm, such as a sash

open switch, or a “fail” signal from the building airflow system.

 Alarm Disable allows an external signal to prevent a “low” airflow alarm from

occurring.

 Night Setback allows an external signal to prevent a “low” airflow alarm from

occurring (not any different from Alarm Disable above other than the terminology.)

 Contact Labconco for ordering information on this special PCB.

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Chapter 6: Maintaining Your Filtered Enclosure

Enclosure Operating In-Flow

Speed

Alarm Condition Set Point

Speed

100 ± 10 fpm

70-90 fpm

90 ± 10 fpm

60-80 fpm

80 ± 10 fpm

50-70 fpm

70 ± 10 fpm

50-60 fpm

60 ± 10 fpm

50 fpm

Calibration

1. Ensure the flow switch and alarm circuit board are installed and

operational.

2. Allow the enclosure to operate for at least two minutes.

3. If factory installed, the monitor will alarm at 60±10 fpm with the inflow

velocity set at 90±10 fpm.

4. To change the factory setting, set the inflow velocity required by your

Safety Officer to the desired alarm condition using the speed control adjustment procedure outlined in Chapter 6. Once the alarm condition is set, use a small screwdriver to turn the adjustment screw on the airflow

switch counterclockwise (facing the screw) until the “low” airflow red

LED lights and the audible flow alarm sounds.

5. Adjust the inflow velocity to the nominal operating point required by your

Safety Officer.

6. Over time the HEPA filter will load and eventually slow the inflow

velocity. Once the alarm condition is met, simply increase the speed control outlined in Chapter 6 or replace the HEPA filter if the speed control is maximized.

7. The table below lists typical alarm conditions based on normal operating

conditions. Typical alarm conditions are set at face velocities of 10 to 30 feet per minute below the normal operating conditions due to supply air and exhaust air fluctuations, as well as room air cross drafts. Consult your Safety Officer for proper operating speeds.

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Chapter 6: Maintaining Your Filtered Enclosure

Figure 6-2

Guardian Airflow Monitor (LED) with Airflow Switch

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Chapter 6: Maintaining Your Filtered Enclosure

Determination of When to Replace Odor Control Carbon Filters and How to Replace

The carbon filters MUST be replaced when any one of the following two conditions are met:

1. The filtered enclosure outlet (exhaust) concentration approaches the inlet

concentration, indicating filter saturation.

2. The odor in the work area becomes intolerable or the concentration of the

chemical in the work area is greater than the TWA.

There are four means of determining when it is time to change the carbon filters (not shown in the order of preference).

Odor - A person’s sensitivity to odor, tolerance of odor and their comfort level under odoriferous conditions vary with the individual. While odor is an indicator that chemicals are passing through the carbon filter, several points need to be understood:

 Odor within the room is not necessarily an indication of saturation or

hazardous exposure concentrations.

 Odor can be used as a prompt to sample the chemical concentration on the exit

side of the carbon filter.

 Organic chemicals approved for use in the filtered enclosure have odors that

are detectable before reaching the time weighted exposure limits.

Detection Tubes - Color change indicators can be used to measure the concentration of the chemical at the exit side of the carbon filter or in the outlet exhaust. A kit including syringe pump and flexible tubing can be purchased as an accessory from Labconco (Catalog # 6924900). Labconco Customer Service Representatives are supplied with detector tube catalog numbers, as well as telephone numbers to direct you to where to purchase these items.

For Organic, Formaldehyde and Ammonia, chemical specific detector tubes should be purchased when installing fresh filters. Each kit contains instructions on how many strokes of the syringe are required to obtain the stated sensitivity. The sampling syringe is connected to the filtered enclosure exhaust. Connect the syringe to the detector tube while the system is running and pull the air through the tube with the syringe. Each stroke of the syringe represents a 100-ml sample and corresponds to the number of strokes necessary to give the indicated color changes.

Due to the wide variety of organics and varying TWA’s, it is recommended that

specific detector tubes be purchased directly from Sensidyne, Draeger or your laboratory supply dealer. Alternate detector pumps can also be purchased from your laboratory supply dealers. The vast majority of detector tubes available start measuring at the TWA. When a user observes a color change in the tube, they should replace the filter immediately. If no detector tube for your specific chemical is available, other means of detection must be used.

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Chapter 6: Maintaining Your Filtered Enclosure

Time - For applications that have very consistent inlet concentrations and

operating time, “Time” can be used to anticipate saturation or TWA levels based on prior experience. However, this does not replace the need for sampling. Consult Labconco technical specialist for an estimate of carbon filter life based on chemical usage. Detector tubes, or analytical instrumentation should always be used to determine concentrations in the carbon filter. It is recommended that the carbon filters be checked with detector tubes or other means at intervals of 20% of the total estimated filter life. The exception to the 20% recommendation is formaldehyde and any carcinogen or suspected carcinogen. These more hazardous chemicals must be checked at least every 10% of the total estimated time.

Analytical Instrumentation - This is the most accurate means of measuring concentrations of any chemical. It is the method of choice when no detector tubes are available or the tubes are not sensitive enough to measure at the TWA concentration for the chemical. This method is also to be used to determine saturation when the chemical concentration is below the measurement range of detector tubes.

Odor Control Carbon Filter Replacement Procedure – See Figures 4-1 and 4-2 for Carbon Filter Replacement.

1. The carbon filters are replaced by first removing the two screws, which

hold the clips to secure the upper diffuser screen. Remove the screws, clips and upper diffuser screen.

2. Remove the carbon filter in a careful way to avoid the flow switch and

HEPA filter.

3. Re-install the new carbon filter with the gasket down. Replace the upper

diffuser screen, clips and two screws.

4. The weight of the carbon filter with the gasket down will compress the

gasket.

Calculating Odor Control Carbon Filter Life

Labconco developed a modeling program to estimate the filter life for typical carbon filters. Since filter life is dependent on the chemical used, the airflow, filter size, and the dwell time, refer to the Chemical Guide for the Paramount® Filtered Enclosure. The estimated life for Odor Control carbon filters for the filtered enclosures is conservatively calculated at 50% or half of the published values for the Paramount in the Chemical Guide. For example, if you use isopropyl alcohol to disinfect and use approximately 100 ml per week during 2 hours of use per day then follow these steps to calculate the concentration in parts per million (ppm).

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Chapter 6: Maintaining Your Filtered Enclosure

Model

Size

Filter Size

Pounds of

Formasorb

Carbon

Adsorbed weight of

Formaldehyde

Adsorption

Volume of

Formalin

18 x 18 x 1

7.0

0.7 lbs./318g

796 ml

30 x 18 x 1

12.0

1.2 lbs./545g

1364 ml

42 x 18 x 1

16.8

1.68 lbs./763g

1910 ml

Model

Size

Filter Size

Pounds of

Ammonasorb

II Carbon

Adsorbed weight of

Ammonia

Adsorbed

Volume of 50%

Ammonia

Solution

18 x 18 x 1

9.2

0.92 lbs./418g

836 ml

30 x 18 x 1

13.5

1.35 lbs./613g

1226 ml

42 x 18 x 1

18.9

1.89 lbs./859g

1718 ml

=7.8 weeks before filter saturation

=.17 ml/min

Steps for Calculating PPM and Filter Life

1. Determine the amount of the proposed chemical lost to evaporation over a

given amount of time. For example, if you use isopropyl alcohol and lose approximately 100 ml per week during 2 hours of use per day.

2. Convert the amount lost into ml/min. For this example:

100 ml X 1 week X 10 hours = 100 ml lost week 10 hours use 600 minutes 600 minutes

3. Convert ml/min to PPM by multiplying ml/min by the conversion factor

found in the second to the last column on the right. For isopropyl alcohol .17 x 41 = 7.0 PPM.

4. Find the PPM value on the chart that comes closest to the value you just

calculated in step #3. In this example, round up to 10 PPM, which is close to the calculated 7.0. We may approximate the filter life to be around 155 hours of actual use, but use 50% of this for the Odor Control filters or 78 hours.

5. Insert the estimated filter life into the estimated usage to determine how

long filters will last. 78 hours filter life

10 hours per week use

Formaldehyde only

For formaldehyde, use 10% of the impregnated carbon weight. Formalin is 37% formaldehyde by weight. The density of formalin is 1.08 g/ml.

Ammonia only

For ammonia, use 10% of the impregnated carbon weight. Assume use of a 50% solution of ammonia for these calculations.

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Chapter 6: Maintaining Your Filtered Enclosure

Initial Certification

The filtered enclosure has been certified at the factory for an inflow velocity of 90±10 fpm along with the HEPA Filter Leak Test. The filtered enclosure should be certified for the proper inflow velocity. A HEPA filter leak test should be performed once installed to verify there has not been any damage incurred during transportation.

Re-Certification

Under normal operating conditions, the enclosure should be recertified at least annually if serviced. The certifier should perform the following tests.

 Inflow Velocity Test  HEPA Filter Leak Test

In addition, the following tests should also be performed at the user’s discretion:

 Measure of Line Voltage and Current  Smoke Test to determine proper airflow patterns  Lighting Intensity Test (when appropriate)  Noise Level Test (when appropriate)  Vibration Test (when appropriate)

Fluorescent Light Replacement

1. Disconnect the power.

2. Locate the small light reflector located under the control panel shown in

Figure 6-2.

3. Remove the light reflector support by removing two Phillips screws on the

bottom of the light reflector.

4. Rotate and remove the old fluorescent lamp.

5. Reinstall the new fluorescent lamp and light reflector in reverse order.

6. Power the unit up and try the new fluorescent lamp.

UV Light Replacement

1. Disconnect the power.

2. Locate the UV lamp located inside the enclosure.

3. Surface decontaminate the old UV light before handling it.

4. Rotate and remove the old UV lamp.

5. Reinstall the new UV lamp.

6. Power the unit up and try the new UV lamp (the UV closure panel must be

installed).

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Chapter 6: Maintaining Your Filtered Enclosure

Motorized Impeller Replacement

The motorized impeller must be replaced as a complete unit. When the motorized impeller is replaced, the capacitor may also be replaced. See Appendix A for Replacement Parts Diagram. See Figure 6-5 for an isometric view of the motorized impeller plenum assembly. The HEPA filter rests on top of the motorized impeller assembly.

Do NOT contact blower wheel while still in motion.

NE PAS être en contact avec la roué du ventilateur tant qu’il est en marche.

1. Wear appropriate personal protective equipment to decontaminate the filtered

enclosure and then unplug from the electrical outlet.

2. Remove HEPA filter per the HEPA filter removal procedure outlined in this

chapter.

3. Consult the wiring diagram in Appendix C of the manual and disconnect all the

wires of the motorized impeller. Be sure to connect wires on the new motor in the same way the old motor was wired.

4. Remove four screws in the motor bracket that hold motor bracket to the

supports with the vibration isolation mounts. Remove the motor and bracket.

ATTENTION: High-speed blower. Never operate impeller with housing off.

5. Replace the capacitor with a new one of equal voltage and capacity.

6. Reassemble the new motorized impeller by reversing the assembly steps.

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Chapter 6: Maintaining Your Filtered Enclosure

Motorized Impeller Replacement

Figure 6-3

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Chapter 6: Maintaining Your Filtered Enclosure

Upper Diffuser

Trace Odor Airflow Sensor

Speed Control Replacement

1. Remove the bracket that the speed control is attached to. See Figures 6-1, and

6-2.

2. Remove the two screws holding the speed control using a Phillips screwdriver.

Refer to Appendix A for Replacement Parts Diagram.

3. Disconnect all wires leading to the speed control. Connect wires on new speed

control in the same position as the old speed control.

4. Reassemble to the system in the same position and with the same screws that

were removed earlier.

Trace Odor Carbon Filter Installation

In the event that low volumes of solvents are required, a Trace Odor Carbon Filter can be installed.

1. Remove the screws (2) located on each side of the upper diffuser.

2. Lift and remove the upper diffuser.

3. Place the Trace Odor Carbon Filter on top of the HEPA filter with the gasket-

side DOWN. Use care when maneuvering past the airflow sensor. Be careful not to harm the HEPA filter media that is exposed.

4. Replace the upper diffuser

5. Replace all screws.

Carbon Filter

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Catalog #

Description

Dimensions (W x D x H)

3909900

Black, 2-foot wide

24" x 29" x 1"

3909901

Black, 3-foot wide

36" x 29" x 1"

3909902

Black, 4-foot wide

48" x 29" x 1"

3909903

Gray, 2-foot wide

24" x 29" x 1"

3909904

Gray, 3-foot wide

36" x 29" x 1"

3909905

Gray, 4-foot wide

48" x 29" x 1"

CHAPTER 7

ACCESSORIZING AND

MODIFYING YOUR FILTERED ENCLOSURE

There are several ways to accessorize and modify the filtered enclosure for your individual requirements. These include the addition of accessory work surfaces, airflow monitors; exhaust transition kits, remote blowers, exhaust dampers, filters, storage cabinets and utility shelf kit.

1. Work Surfaces

An optional dished work surface is available to attach to the filtered enclosure.

Dished work surfaces are contoured to fit the dimensions of the filtered enclosures to contain spills. Epoxy is chemical resistant. See Figure 3-1 for installation.

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Chapter 7: Accessorizing & Modifying Your Filtered Enclosure

Description

Order Part #’s

Individual Parts Required

Guardian Airflow Monitor 115V or 230V

1 ea.

3811500

Airflow Monitor Printed Circuit Board

1 ea.

3910700

Airflow Sensor w/ Connector

2 ea.

1885405

#6-32 x .31" Phillips machine screw to mount Airflow Monitor Circuit Board

2 ea.

1891808

#6-32 x .50" Phillips thread cutting screw to mount Airflow Sensor

Part

Description

3924400

2' Thimble Exhaust Connection

3924401

3' Thimble Exhaust Connection

3924402

4' Thimble Exhaust Connection

3924405

2' Canopy Exhaust Connection (5") – to FilterMate

3924406

3' Canopy Exhaust Connection (5") – to FilterMate

3924407

4' Canopy Exhaust Connection (5") – to FilterMate

CFM @ Static Pressure-Inches of H2O

S.P.

0.0"

0.125"

0.25"

0.50"

0.75"

0.87"

CFM

595

560

515

420

300

167

2. Guardian™ LED Airflow Monitor

The Guardian LED Airflow Monitor allows you to continuously monitor airflow through the enclosure. The Guardian LED monitor can be placed on any Purifier enclosure.

3. Thimble Exhaust Connections for Ducting to Outside

Thimble Exhaust connects to the enclosures with integral motorized impellers from the top so the duct can be routed up and outside the building. The transition is available for 6" nominal duct. The Thimble Exhaust Connection should be sealed to the top of the enclosure with silicone sealant to prevent leaks. See figure 3-2 and figure B-2.

Order the following:

4. Remote Blowers

For Purifier Class I Enclosure

The remote blower has 1/4 hp direct drive motor and corrosion-resistant epoxy-coated steel housing and wheel with blower inlet of 5.94" OD. sized to accept 6" nominal PVC duct. Includes a blower transition adapter outlet for 6" PVC duct with integral back draft damper.

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Chapter 7: Accessorizing and Modifying Your Filtered Enclosure

Used With

Catalog #

Description

Shipping Wt. (lbs./kg.)

Purifier Class I 3716000

Remote Blower, 115V, 60 Hz. 4.4 amps

35/16

3716001

Remote Blower, 115/230V, 50 Hz,

5.6/2.8 amps

35/15

CFM @ Static Pressure-Inches of H2O

S.P.

0.0"

0.125"

0.25"

0.50"

0.75"

0.87"

CFM

595

560

515

420

300

167

Catalog #

Description

Shipping Wt.

(lbs./kg.)

Used with Purifier

HEPA Filtered

Enclosure

4863500

Remote Blower, 115 V, 60 Hz. 4.4 amps

35/16

4863501

Remote Blower, 115/230 V, 50 Hz, 5.6/2.8 amps

35/16

7053501

Explosion-Proof Remote Blower, 115 V, 60 Hz,

4.4 amps

40/18

Figure 7-1

For Purifier HEPA Filtered Enclosure

Has a 1/4 hp direct drive motor and corrosion-resistant epoxy-coated steel housing and wheel with blower inlet of 6.00" ID. Outlet dimensions are 4.25" x 7.38" OD.

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Chapter 7: Accessorizing & Modifying Your Filtered Enclosure

Catalog #

Description

3924000

6" Epoxy Coated Steel In-Line adjustable damper

4724200

6" PVC In-line adjustable damper

Width

Catalog #

Size

3707900

18" x 18" x 3.31"

3707901

30" x 18" x 3.31"

3707902

42" x 18" x 3.31"

Filter Classification and Size

Catalog #

X Pounds

2' Organic 18 x 18 x 1

3937200

5.5 lbs. activated

3' Organic 30 x 18 x 1

3937300

9.2 lbs. activated

4' Organic 42 x 18 x 1

3937400

12.9 lbs. activated

2' Formaldehyde 18 x 18 x 1

3937201

7.0 lbs impregnated

3' Formaldehyde 30 x 18 x 1

3937301

12.0 lbs. impregnated

4' Formaldehyde 42 x 18 x 1

3937401

16.8 lbs. impregnated

2' Ammonia & Amines 18 x 18 x 1

3937202

8.0 lbs. impregnated

3' Ammonia & Amines 30 x 18 x 1

3937302

13.5 lbs. impregnated

4' Ammonia & Amines 42 x 18 x 1

3937402

18.9 lbs. impregnated

5. Exhaust Dampers

Exhaust dampers allow adjustments required to maintain proper airflow for roof-mounted blowers or house exhaust systems.

6. Accessory Filters HEPA Filter

HEPA Filter is 99.99% efficient on particles 0.3 micron.

Odor Control Carbon Filter Provides granular activated carbon or impregnated carbon used for Odor Control applications on integral motorized impeller models exhausted back into the room.

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Chapter 7: Accessorizing and Modifying Your Filtered Enclosure

SOLVENT

ACID

Size/Description

Dual Doors

Right Hinge

Left Hinge

Dual Doors

Right Hinge

Left Hinge

48"

9902000

9901000

36"

9902100

9901100

30"

9902200

9901200

24" - 9902300

9902400

9901300

9901500

18" - - - -

9901400

9901600

12" - - - - - -

48" w/Self-Closing Doors

9903000

- - - - -

36" w/Self-Closing Doors

9903100

- - - - -

30" w/Self-Closing Doors

9903200

- - - - -

24" w/Self-Closing Doors

9903300

9903400

- - -

24" ADA

9906000

9906100

9905000

9905200

24" ADA w/Self-Closing Doors

9906200

9906300

- - -

18" ADA - - - -

9905100

9905300

12" ADA - - - - - -

STANDARD BASE

VACUUM PUMP

Size/Description

Dual Doors

Right Hinge

Left Hinge

Dual Doors

Right Hinge

Left Hinge

48"

9900000

- - - - -

36"

9900100

- - - - -

30"

9900200

- - - - -

24" - 9900300

9900600

- - -

18" - 9900400

9900700

9907000

9907100

12" - 9900500

9900800

- - -

48" w/Self-Closing Doors

- - - - -

36" w/Self-Closing Doors

- - - - -

30" w/Self-Closing Doors

- - - - -

24" w/Self-Closing Doors

- - - - -

24" ADA

9904000

9904300

- - -

24" ADA w/Self-Closing Doors

- - - - -

18" ADA

9904100

9904400

- - -

12" ADA

9904200

9904500

- - -

7. Storage Cabinets

8. Utility Shelf Kit

Labconco offers a utility shelf kit part number 3925000 consisting of three shelves to hold items inside the enclosure. One shelf may be used for the printer, one shelf for spatulas and weigh brushes, and one miscellaneous shelf. The shelves hang from slots in the rear baffle.

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Chapter 7: Accessorizing & Modifying Your Filtered Enclosure

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PROBLEM

CAUSE

CORRECTIVE ACTION

Contaminants outside of enclosure.

Improper user techniques for the enclosure.

See “Certifying the Enclosure” Chapter 3 and “Safety Precautions” Chapter 4 sections

in the manual. (Ref. Appendix D).

Restriction of the baffle air slots or blockage of the exhaust outlet.

Remove obstruction to ensure that all air slots and the exhaust outlet are unobstructed.

External factors are disrupting the enclosure airflow patterns or acting as a source of contamination.

See “Location Requirements” Chapter 2,

“Certifying the Enclosure” Chapter 3, and “Safety Precautions” Chapter 4 sections of

this manual. (Ref. Appendix D).

Enclosure has improper face velocity.

Have enclosure certified and check exhaust system. Check HEPA filters for loading. Adjust enclosure speed control. Enclosure should have an average face velocity of 60100 fpm for weighing procedures and 75105 fpm for biohazardous procedures.

CHAPTER 8 TROUBLESHOOTING AND

SERVICER OPERATING LOG

Refer to the following table if your filtered enclosure fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance.

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Chapter 8: Troubleshooting and Servicer Operating Log

PROBLEM

CAUSE

CORRECTIVE ACTION

Blower won’t

operate.

Unit not plugged into outlet.

Plug the enclosure into appropriate electrical service.

Circuit breaker(s) or Ground Fault Interrupter.

Reset circuit breaker. Blower wiring is disconnected.

Inspect blower wiring.

Blower switch is defective.

Replace switch.

Motorized impeller or blower is defective.

Replace motorized impeller or blower. See Chapter 6.

Low face velocity or poor containment of contaminants.

Enclosure sash not closed.

Close sash to the lowest position.

HEPA filter clogged.

Replace HEPA filter or increase speed.

Carbon filter loaded with chemicals.

Replace carbon filter.

Blower and lights won’t operate.

Unit not plugged into outlet.

Plug enclosure into appropriate electrical service.

Circuit breaker(s) tripped.

Reset or replace circuit breaker.

Light does not work.

Lamp not installed properly.

Inspect lamp installation.

Lamp wiring disconnected.

Inspect lamp wiring. Defective lamp.

Replace lamp.

Light switch is defective.

Replace light switch.

Defective electronic ballast.

Replace ballast.

UV closure panel not installed .

Install UV closure panel (UV lights only).

Magnetic switch for UV closure panel defective.

Replace switch (UV lights only). UV timer circuit board defective.

Replace circuit board (UV lights only).

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PROBLEM

CAUSE

CORRECTIVE ACTION

Airflow Monitor Malfunction

No power. No lights. No display.

Power cable to circuit board is disconnected and needs to be connected. Sensor cable needs to be connected. Verify that all airflow monitor interface cables are connected. Check fuses on enclosure.

No audible alarm.

Alarm has been temporarily silenced using “SILENCE ALARM”.

Wrong alarm set point.

Airflow monitor was not properly adjusted. Repeat calibration steps outlined in this manual in Chapter 6.

Constant audible alarm.

Check airflow and calibration of airflow monitor. See Chapter 6.

Continuous alarm.

Check the face velocity of the enclosure as the airflow of the system may have changed. If incorrect, adjust the speed control to increase face velocity. The HEPA filter may have become loaded. If face velocity is correct, calibrate the airflow monitor outlined in this manual in Chapter 6.

Monitor alarms; air way to airflow monitor sensor is blocked by insects, dust or debris.

Lightly clean the airway with clean air. Be careful not to touch sensitive electrical components.

Audible disable will not stay operational.

An alarm condition must be continuously present before the audible alarm can be silenced. If flow conditions fluctuate near the alarm set point, the airflow monitor will automatically reset it. Action should be taken to bring the enclosure airflow into proper operating parameters or adjust the alarm set point lower.

Chapter 8: Troubleshooting and Servicer Operating Log

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Chapter 8: Troubleshooting and Servicer Operating Log

Date

Comments

Customer Name

Model Number

Date Installed

Serial Number

Unit Location

Service Operating Record Log

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APPENDIX A

FILTERED ENCLOSURE

COMPONENTS AND REPLACEMENT PARTS

The components that are available for your filtered enclosure are listed. The parts shown are the most commonly requested. If other parts are required, please contact Product Service.

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Appendix A: Filtered Enclosure Components and Replacement Parts

Item

Qty.

Part Number

Description

1 1 3911000

Glass, Side

2A 1 3911900

Glass, Sash 2'

2B 1 3911901

Glass, Sash 3'

2C 1 3911902

Glass, Sash 4'

3A 1 3991500

Sash Assembly, 2'

3B 1 3991501

Sash Assembly, 3'

3C 1 3991502

Sash Assembly, 4'

4A 1 3905600

Lower Air Foil, 2'

4B 1 3905601

Lower Air Foil, 3'

4C 1 3905602

Lower Air Foil, 4'

5A 1 3907400

Bracket, Air Foil R.H.

5B 1 3907401

Bracket, Air Foil L.H.

6A 1 3907500

Stop, Sash R.H.

6B 1 3907501

Stop, Sash L.H.

7A 1 6916500

Latch, Sash

7B 1 3906700

Bracket, Latch

7C 1 1927403

Spring, Compression Latch

7D 2 1893206

Screw, 8-32 x .38" Lg. Phillips Pan S.S. Type F

9A 2 1889316

Screw, 10-24 x 1.00 PH Phil S.S.

9B 2 1912108

Washer, Plastic .194 ID. x .380 OD

9C 2 7868402

Bushing, Spacer .31 x .63

1.9 Ft

6913700

Wiper, Sash Seal

11A 2 1936800

Bushing, Heyco closed 1.50 Dia.

11B 2 1934601

Bushing, Heyco w/ Flex Shutter 1.50 Dia.

12A 1 3915401

Side Air Foil

12B 2 1889912

Screw, #6-32 x .75" Oval Hd. Type F (S.S.)

13A 1 3907700

Reflector, Light 2'

13B 1 3907701

Reflector, Light 3'

13C 1 3907702

Reflector, Light 4'

13D 2 1889308

Screw, Mach. 10-24 x .50 PH Phillips

14A 1 3708300

Lamp, Fluorescent 2' Enclosure (F15T8-SP35)

14B 1 9721902

Lamp, Fluorescent 3' Enclosure (F17T8)

14C 1 9721901

Lamp, Fluorescent 4' Enclosure (F25T8)

15A 1 1270100

Lamp, UV 2' or 3' Enclosure (G15T8)

15B 1 1271300

Lamp, UV 4' enclosure (G30T8)

16A 1 3911700

Bracket, Right Reflector Support

16B 1 3911701

Bracket, Left Reflector Support

16C 4 1893108

Screw, #8 x 1/2 AB Hex Washer Head

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Appendix A: Filtered Enclosure Components and Replacement Parts

Item

Qty.

Part Number

Description

17A 1 3918510

Wiring Harness Main, 115V

17B 1 3918610

Wiring Harness Main, 230V

18A 1 3811500

Guardian Airflow Monitor Printed Circuit Board

18B 2 1885405

Screw Mach #6-32 x .31 PH Phil. S.S.

19A 1 3926600

Label, Front Class I

19B 1 3926700

Label, Front HEPA

20A 1 3910700

Airflow Switch

20B 2 1891808

Screw, Thread Cutting #6-32 x .50 PH

21A 1 3906200

Cover Plate for HEPA 2'

21B 1 3906201

Cover Plate for HEPA 3'

21C 1 3906202

Cover Plate for HEPA 4'

21D

Multiple

1905617

Nut, KEPS 10-24

22A

1 or 2

1307000

Switch, Rocker (2 position)

22B 1 1306300

Switch, Rocker (3 position) – UV Light Only

23A

1 or 2

3904800

Front Panel, 2'

23B 1 3904801

Front Panel, 3'

23C 1 3904802

Front Panel, 4'

23D 1 4472400

Screw, Thread cut 10-24 x .50 Type F PH Phillips

24A 1 3916500

Impeller, Motorized 2' or 3' Enclosure, 115V

24B 1 3916501

Impeller, Motorized 2' or 3' Enclosure, 230V

24C 1 3903300

Impeller, Motorized 4', 115V

24D 1 3903301

Impeller, Motorized 4', 230V

24E 4 1601800

Vibration Isolator Mount

25A 1 1306900

Capacitor, 2', 115V 10 MFD

25B 1 1306900

Capacitor, 3', 115V 10 MFD

25C 1 1306600

Capacitor, 4', 115V 15 MFD

25D 2 1306800

Capacitor, 2', 230V 4 MFD (effective 2MFD)

25E 2 1306800

Capacitor, 3', 230V 4 MFD (effective 2MFD)

25F 1 1306800

Capacitor, 4', 230V 4 MFD

26A 1 1295510

Ballast, Fluorescent 2', 115V

26B 1 1295510

Ballast, Fluorescent 2', 230V

26C 1 1294000

Ballast, Fluorescent 3' or 4', 115V or 230V

27A 1 1295510

Ballast, UV 2' or 3', 115V

27B 1 1295510

Ballast, UV 2' or 3', 230V

27C 1 1295700

Ballast, UV 4', 115V

27D 1 1295700

Ballast, UV 4', 230V

28A 1 1325500

Speed Control, 115V

28B 1 1325501

Speed Control, 230V

29A 1 3910800

Upper Diffuser Screen 2'

29B 1 3910801

Upper Diffuser Screen 3'

29C 1 3910802

Upper Diffuser Screen 4'

30A 2 3916700

Clip, Upper Diffuser

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Appendix A: Filtered Enclosure Components and Replacement Parts

Item

Qty.

Part Number

Description

30B 2 1893708

Screw, #10 x .50 PH Phil. Thd. Forming

31A 2 1881196

Capscrew, 3/8 –16 x 6.0 Lg. Hx. Hd.

31B 2 1924403

Sealing Washer 3/8

32A 1 3707900

HEPA Filter (2') 18 x 18 x 3.32 w/ Gasket

32B 1 3707901

HEPA Filter (3') 30 x 18 x 3.32 w/ Gasket

32C 1 3707902

HEPA Filter (4') 42 x 18 x 3.32 w/ Gasket

33A 1 3937200

2' Odor Control Organic Vapor Carbon Filter

33B 1 3937201

2' Odor Control Formaldehyde Carbon Filter

33C 1 3937202

2' Odor Control Ammonia Carbon Filter

33D 1 3937300

3' Odor Control Organic Vapor Carbon Filter

33E 1 3937301

3' Odor Control Formaldehyde Carbon Filter

33F 1 3937302

3' Odor Control Ammonia Carbon Filter

33G 1 3937400

4' Odor Control Organic Vapor Carbon Filter

33H 1 3937401

4' Odor Control Formaldehyde Carbon Filter

33I 1 3937402

4' Odor Control Ammonia Carbon Filter

34 1 7907802

Digital Monitor Outlet, 115V only, 230V connected at laboratory outlet location.

35A 1 1333800

IEC Power Inlet

35B 1 1305800

Power Cord, 115V, Right Angle

35C 1 1334200

Power Cord, 230V, Right Angle

36A

1 - (115V)

1327200

Circuit Breaker, 3 AMP

36B

2 - (230V)

1327205

Circuit Breaker, 2 AMP

40A 1 1952500

Gauge, Pressure Mini Helic II

40B 1 3667400

Bracket, Gauge

40C 2 1905617

Nut, #10-24 KEPS

40D 2 1551700

Tubing, 3/16" ID x 5/16" OD, PBC 7.00" Lg.

40E

2, 1" Long

3788200

Snubber, Filter Pressure Gauge

41A 1 3560510

Panel, UV Closure 2 Ft.

41B 1 3560511

Panel, UV Closure 3 Ft.

41C 1 3560512

Panel, UV Closure 4 Ft.

42 2 1907002

Tape, hook and latch, fastening

43 1 1322800

Switch assembly, magnetic

44 1 3945302

Circuit board, UV Timer

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Appendix A: Filtered Enclosure Components and Replacement Parts

2 3 4 5 12

Placed

on ledge

above

HEPA

filter

Use for Purifier Class I and Purifier HEPA Filtered Enclosures

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APPENDIX B DIMENSIONS AND EXHAUST OPTIONS

See the following dimensions and exhaust options for all the Purifier Filtered Enclosures.

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Appendix B: Dimensions and Exhaust Options

Figure B-1

Purifier Class I and Purifier HEPA Filtered Enclosure

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Appendix B: Dimensions and Exhaust Options

Figure B-2

Thimble Exhaust Connection

(Optional installation for outside ducting)

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APPENDIX C FILTERED ENCLOSURE

SPECIFICATIONS

This Appendix contains technical information about all the Purifier HEPA Filtered Enclosures including electrical specifications and environmental operating conditions.

Electrical Specifications

 3 Amps, 115V, 50/60 Hz  2 Amps, 230V, 50/60 Hz

Environmental Conditions

 Indoor use only.  Maximum altitude: 6562 feet (2000 meters).  Ambient temperature range: 41° to 104°F (5° to 40°C).  Maximum relative humidity: 80% for temperatures up to 88°F (31°C),

decreasing linearly to 50% relative humidity at 104°F (40°C).

 Main supply voltage fluctuations not to exceed ±10% of the nominal

voltage.

 Transient over-voltages according to Installation Categories II (Over-

voltage Categories per IEC 1010). Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed.

 Used in an environment of Pollution degrees 2 (i.e., where normally only

non-conductive atmospheres are present). Occasionally, however, a temporary conductivity caused by condensation must be expected, in accordance with IEC 664.

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Model Size

Sash Height from Work Surface (inches)

9.44

Total Open Area with Bypass (sq. ft.)

1.45

2.20

2.95

Exhaust Airflow Volume at 90fpm (CFM)

130

200

265

Alarm Airflow Volume at 60 fpm (CFM)

130

175

Initial Mag. Gauge Pressure Reading (inches H2O)

0.20 to .045

0.15 to 0.40

0.10 to 0.35

Number of Laskin Nozzles needed at 10 psig

1 1 1

Exhaust HEPA Filter w/ Gasket Dimensions (inches)

18x18x3.31

30x18x3.31

42x18x3.31

*Motor Horsepower (HP)

0.13

0.30

*Capacitor (MFD, Volts)

10MFD, 115V

(2) 4MFD, 230V

10MFD, 115V

(2) 4MFD, 230V

15MFD, 115V

4MFD, 230V

Fluorescent Lights

F15T8-SP35

F17T8

F25T8

UV Light on Class I only

G15T8

G30T8

APPENDIX D QUICK CHART FOR THE FILTERED ENCLOSURES

To determine the actual face velocity at the sash opening, airflow velocity readings will need to be taken. This should be done across the sash opening of the enclosure in accordance with the Industrial Ventilation Manual. (See Appendix E)

The “average face velocity” is achieved by taking readings in two rows across the

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APPENDIX E REFERENCES ON

VENTILATION, SAFETY,

OCCUPATIONAL HAZARDS, BIOSAFETY AND DECONTAMINATION

Many excellent reference texts and booklets are currently available. The following is a brief listing:

Laboratory Ventilation Standards

Federal Register 29 CFR Part 1910

Non-mandatory recommendations from “Prudent Practices.”

 Fume hoods should have a continuous monitoring device  Face velocities should be between 60-100 linear feet per minute (lfpm)

 Average 2.5 linear feet of hood space per person

Occupational Health and Safety U.S. Department of Labor 200 Constitution Avenue N.W. Washington, DC 20210 (202) 523-1452 www.osha.gov

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Appendix C: Filtered Enclosure Specifications

Industrial Ventilation-ACGIH

 Fume hood face velocities between 60-100 lfpm  Maximum of 125 lfpm for radioisotope hoods  Duct velocities of 1000-2000 fpm for vapors, gasses and smoke  Stack discharge height 1.3-2.0 x building height  Well designed fume hood containment loss, <0.10 ppm

Industrial Ventilation, A Manual of Recommended Practice. 24th Edition, 2001 American Conference of Governmental Industrial Hygienists 1330 Kemper Meadow drive Cincinnati, OH 45240-1634 (513) 742-2020 www.acgih.org

ASHRAE 110-1995 Method of Testing Performance of Fume Hoods

Evaluates fume hood’s containment characteristics

 Three part test: Smoke generation, Face velocity profile, Tracer gas release @ 4 liters

per minute

 Rated As Manufactured (AM), As Installed (AI) and As Used (AU)

American Society of Heating, Refrigerating, and Air Conditioning Engineers 1791 Tullie Circle N.E. Atlanta, GA 30329 (404) 636-8400 www.ashrae.org

ANSI Z9.5-1993 Laboratory Standard

Covers entire laboratory ventilation system.

 Vertical stack discharge @ 2000-3000 fpm  New and remodeled hoods shall have a monitoring device  Ductless hoods should only be used with non-hazardous materials  Fume hood face velocities between 80 – 120 fpm

American Industrial Hygiene Association 2700 Prosperity Avenue, Suite 250 Fairfax, VA 22031 (703) 849-8888 www.aiha.org

SEFA 1-2002

 Fume hood face velocities based on toxicity levels of chemicals

Class A – 125 to 150 fpm Class B – 80 to100 fpm Class C – 75-to 80 fpm

 Test method – face velocity profile and smoke generation

Scientific Equipment & Furniture Association 1028 Duchess Drive McLean, VA 22102 (703) 538-6007 www.sefalabs.com

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NFPA 45 – 2002 Fire Protection for Laboratories Using Chemicals

 Laboratory hoods should not be relied on for explosion protection  Exhaust air from fume hoods should not be recirculated  Services should be external to the hood  Canopy hoods only for non-hazardous applications  Materials of construction should have flame spread of 25 or less  80 to 120 fpm to prevent escape

NFPA 30 – 2000 Flammable and Combustible Liquids Code

 Approved cabinets may be metal or wood  Vent location on cabinets are required  Venting of cabinets not a requirement

National Fire Protection Association 1 Batterymarch Park P.O. Box 9101 Quincy, MA 02269-9101 (800) 344-3555 www.nfpa.org

General References

American Conference of Governmental Industrial Hygienists. Industrial Ventilation, A Manual of Recommended Practice, Cincinnati, OH

ASHRAE Standard Committee. ASHRAE Standard Atlanta: ASHRAE Publications Sales Department, 1995

British Standards Institution, Laboratory Fume Cupboards. Parts 1, 2 and 3, London: 1990

Department of Labor, Occupational Safety and Health Administration, 29 CFR Part 1910, Occupational Exposures to Hazardous Chemicals in Laboratories, Final Rule. Vol. 55, No. 21. Washington D.C.:1990

DiBerardinis. L. et al. Guides for Laboratory Design, Health and Safety Considerations. Wiley & Sons, 1987

McDermott, Henry, Handbook of Ventilation for Contaminant Control, 2nd Edition. Butterworth Publishers, 1985.

Miller, Brinton M. et al. Laboratory Safety: Principles and Practices. American Society for Microbiology, Washington, D.C.: 1986

NIH Guidelines for the Laboratory Use of Chemical Carcinogens. NIH Publication No. 81-2385.

Rayburn, Stephen R. The Foundation of Laboratory Safety, A Guide for the Biomedical Laboratory. Springer-Verlag, New York: 1990

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Appendix C: Filtered Enclosure Specifications

Sax, N. Irving and Lewis, JR., Richard J. Rapid Guide to Hazardous Chemicals in the Workplace. Van Nostrand Reinhold, 1987.

Schilt, Alfred A. Perchloric Acid and Perchlorates. The G. Frederick Smith Chemical Company, Columbus, OH: 1979.

Steere, Norman. CRC Handbook of Laboratory Safety, 2nd Edition. CRC Press, 1971.

Collins, C.H., 1988. Laboratory Acquired Infections, History, Incidence, Causes and Prevention, 2nd ed., Butterworths, London.

Kruse, R.H., WH. Puckett and J. H. Richardson. 1991. Biological Safety Cabinetry, Clin. Microbiol. Rev. 4:207-241.

Miller, C.D., D.H.M. Groschel, J. H. Richardson, D. Vesley, J. R. Songer, R. D. Housewright and W. E. Barkley. 1986. Laboratory Safety, Principles and Practices, American Society for Microbiology, Washington, D.D.

National Research Council (U.S.) Committee on Hazardous Biological Substances in the Laboratory. 1989. Biosafety in the Laboratory. Prudent Practices for Handling and Disposal of Infectious Materials. National Academy Press, Washington, D.C.

Rayburn, S.R. 1990. The Foundations of Laboratory Safety, Springer-Verlag, New York.

U. S. Department of Health, Education and Welfare. Formaldehyde Decontamination of Laminar Flow Biological Safety Cabinets, National Institutes of Health, Division of Safety, Bethesda, MD 20892.

U. S. Department of Health, Education and Welfare. Effective use of a Laminar Flow Biological Safe Cabinet. National Audiovisual Center (GSA), Sales Branch, Catalog No., NAC 005133, Washington, D.C.

U. S. Department of Health, Education and Welfare. 1975. Selecting a Biological Safety Cabinet. National Audiovisual Center (GSA), Sales Branch, Catalog No. NAC 000709, Washington, D.C.

U. S. Department of Health and Human Services. 1999. Biosafety in Microbiological and Biomedical Laboratories, 4th ed. U.S. Department of Health and Human Services, Publication No. (CDC) 93-8395. U. S. Government Printing Office, Washington, D.C.

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Labconco Class I Enclosure User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual