Page 1
Original instructions
User’s Manual
CentriVap®Complete™
Models
73150 Series
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Labconco Corporation
8811 Prospect Avenue
Kansas City, MO 64132-2696
800-821-5525, 816-333-8811
Fax 816-363-0130
E-MAIL labconco@labconco.com
HOME PAGE www.labconco.com
Please read the User’s Manual before operating the equipment.
Page 2
Original instructions
Copyright © 2012, 2013 Labconco Corporation. All rights reserved.
The information contained in this manual and the accompanying products are copyrighted and all rights
reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design
changes without obligation to notify any person or entity of such change.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.
The warranty for all Labconco products will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner, except the following;
• Purifier® Logic® Biological Safety Cabinets and PuriCare® Lab Animal Research Stations
carry a three-year warranty from date of installation or four years from date of shipment from
Labconco, whichever is sooner.
• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from date
of installation or three years from date of shipment from Labconco, whichever is sooner.
• Blood Drawing Chairs carry a ten year warranty.
• Carts carry a lifetime warranty.
• Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or
replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement may
be altered by a specific published amendment. No individual has authorization to alter the provisions
of this warranty policy or its amendments. Lamps and filters are not covered by this warranty.
Damage due to corrosion or accidental breakage is not covered.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier.
Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525
or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #7314000, Rev. B
ECO H435
Page 3
Original instructions
T
AABBLLEE
T
CHAPTER 1: INTRODUCTION 1
Safety Symbols 2
CHAPTER 2: PREREQUISITES 3
Electrical Requirements 3
Location and Exhaust Requirements 4
Space Requirements 4
CHAPTER 3: GETTING STARTED 5
Unpacking Your CentriVap 6
CentriVap Components 6
Setting Up Your CentriVap 7
Emergency Access Into the Chamber 7
Exhaust Port 8
Electrical Connection 8
Rotor Installation 8
Chemical Resistance of CentriVap Components 8
Solvent Safety Precautions 9
CHAPTER 4: USING YOUR CENTRIVAP COMPLETE 11
Planning 12
Glassware Selection 12
Loading Glassware Into the CentriVap 12
Temperature Setting Guidelines 12
Time Setting Guidelines 12
Heat Boost Operation 13
CentriVap Controls 13
Operating the CentriVap Controls 14
Operating The CentriVap 15
CentriZap Strobe Light 16
Operational Notes 16
Interrupting a Cycle After it Has Begun 17
Gas Ballast 17
Safety Precautions 17
O
O
FF
C
C
OONNTTEENNTTSS
Page 4
Original instructions
CHAPTER 5: MAINTAINING YOUR CENTRIVAP COMPLETE 18
CHAPTER 6: ACCESSORIES FOR YOUR CENTRIVAP
COMPLETE 20
Installing a Vacuum Gauge 22
Installing a Secondary Chemical Trap 23
CHAPTER 7: TROUBLESHOOTING 24
APPENDIX A: CENTRIVAP COMPONENTS 27
APPENDIX B: CENTRIVAP DIMENSIONS 29
APPENDIX C: CENTRIVAP SPECIFICATIONS 30
Electrical Specifications 30
Environmental Conditions 31
Evaporation Rates 32
DECLARATION OF CONFORMITY 34
Page 5
Original instructions
CChhaapptteerr 11::
IInnttrroodduuccttiioonn
Congratulations on your purchase of a Labconco CentriVap Complete. Models
are available for operation on 115V or 230V.
The CentriVap Complete combines a concentrator, cold trap, and vacuum pump
in one cabinet. The concentrator uses centrifugal force with heat and vacuum to
rapidly evaporate and condense solvents from biological and analytical samples.
Centrifugation eliminates bumping and foaming as vacuum is applied and also
concentrates the solute in the bottom of the vial. This allows recovery of solutes
from sample volumes as small as a few microliters. The concentrator is equipped
with a 300 watt heater to speed evaporation by warming the chamber during
processing. The microprocessor delays the start of the vacuum pump until the
rotor reaches operating speed. A safety switch prevents the concentrator from
starting when the lid is in the open position and a latch prevents the lid from being
opened while the rotor is turning.
The microprocessor, which controls the operation of the heater and the motor,
provides excellent regulation and reproducibility of protocols. The end of the run
can be signaled by a manually preset timer. Nine different protocols can be stored
in memory so exact test parameters can be easily repeated.
As an integral part at the CentriVap Complete, the cold trap can be used to trap
moisture, vapors and corrosive fumes as they evaporate from the samples.
Standard models can be used for aqueous and organic applications. Acid resistant
models are recommended for corrosive applications. The 230V models comply
with CE regulations.
Product Service 1-800-522-7658
1
Page 6
Original instructions
Chapter 1: Introduction
Safety Symbols
Your CentriVap Complete was designed with safety in mind, however conditions
may exist that could be hazardous.
Throughout this manual potentially hazardous conditions are identified using the
following words and symbols.
It is important that you understand the warnings listed throughout this manual
before you operate the CentriVap Complete.
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Page 7
Original instructions
CChhaapptteerr 22::
PPrreerreeqquuiissiitteess
Before you install your CentriVap, you need to prepare your site for installation.
Carefully examine the location where you intend to install your CentriVap. You
must be certain that the area is level and of solid construction. In addition, an
exhaust means must be provided. An electrical source must be located near the
installation site.
Carefully read this chapter to learn:
• The electrical supply requirements.
• The exhaust requirements.
• The vacuum pump requirements.
Refer to Appendix C: CentriVap Specifications for electrical and environmental
conditions, specifications and requirements.
If the unit is not operated as specified in this manual it may impair the protection
provided by the unit.
Electrical Requirements
The CentriVap Complete requires a dedicated grounded electrical outlet. This
outlet requires a 15 Amp circuit breaker or fuse for models rated at 115V (60 Hz).
An 8 Amp circuit breaker or fuse is required for models rated at 230V (50/60 Hz).
Various models are available for use in different locations throughout the world.
Each has a unique plug suitable for the specific location. See CentriVap
Components in Chapter 3 for detailed plug specifications. If the plug supplied
with the CentriVap does not match with the available receptacle, remove this plug
and replace it with an approved plug of the suitable style.
It is recommend that an emergency switch for disconnecting the mains in the case
of a malfunction be located remote from the CentriVap, preferably outside the
room in which the CentriVap is housed, or adjacent to the exit from that room.
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Page 8
Original instructions
Chapter 2: Prerequisites
Do not position the CentriVap so that it is difficult to remove
the power cord from the unit or to operate the power switch.
!
Do not use any detachable power cord that is not adequately
rated for the unit.
Location and Exhaust Requirements
The CentriVap Complete should be located on a surface that is stable, flat and
level.
WARNING: The CentriVap should be located within a fume hood if
hazardous or flammable solvents are used. Heating of materials could lead
to the liberation of hazardous gases. In all cases, regardless of the solvent
used, it is strongly recommended that the vacuum pump is vented in a fume
hood. An accessory secondary trap is available to minimize the exhausting of
solvents into the atmosphere. This does not, however, negate the need to
exhaust the vacuum pump into a fume hood. Failure to properly vent the
CentriVap will expose personnel to potentially harmful chemicals.
The CentriVap has not been evaluated by an approval agency for the use of
biological, radio toxins or flammable liquids or materials.
Space Requirements
Refer to Appendix C: CentriVap Specifications for dimensional drawings of the
CentriVap.
No person or any hazardous material should be within 12 inches of the CentriVap
while it is operating.
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Page 9
Original instructions
CChhaapptteerr 33::
GGeettttiinngg SSttaarrtteedd
Now that the site for your CentriVap Complete is properly prepared, you are
ready to unpack, inspect, install, and test your CentriVap Complete. Read this
chapter to learn how to:
• Unpack and move your CentriVap Complete.
• Set up your CentriVap Complete.
• Connect the electrical supply source to your CentriVap Complete.
• Properly exhaust your CentriVap Complete.
• Safely use solvents with your CentriVap Complete.
CAUTION: The CentriVap Complete weighs 145 lbs. (66 Kg). The carton
allows for lifting with a mechanical lift truck or hand truck. If you must lift
the CentriVap manually, use at least two (2) persons and follow safe lifting
guidelines.
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Page 10
Original instructions
Chapter 3: Getting Started
Unpacking Your CentriVap
Carefully unpack your CentriVap and inspect it for damage that may have
occurred in transit. If your CentriVap is damaged, notify the delivery carrier
immediately and retain the entire shipment intact for inspection by the carrier.
The United States Interstate Commerce Commission rules require that claims be
filed with the delivery carrier within fifteen (15) days of delivery.
NOTE: Do not return goods without the prior authorization of Labconco.
Unauthorized returns will not be accepted. If your CentriVap was damaged
in transit, you must file a claim directly with the freight carrier. Labconco
Corporation and its dealers are not responsible for shipping damage.
Do not discard the carton or packing material for your CentriVap until you have
checked all of the components and installed and tested the CentriVap.
CentriVap Components
As previously mentioned, the CentriVap Complete is available in 115V or 230V.
Locate the model of CentriVap you received in the following table. Verify that
the components listed are present and undamaged.
Power C ords
1334500
1336100
Catalog # Description Volts Hz
7315020 CENTRIVAP COMPLETE (US) 115 60 X
7315021 CENTRIVAP COMPLETE ACID RESISTANT (US) 115 60 X
7315022 CENTRIVAP COMPLETE W/ HEAT BOOST (US) 115 60 X
7315023 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (US) 115 60 X
7315030 CENTRIVAP COMPLETE (EU) 230 50 X
7315031 CENTRIVAP COMPLETE ACID RESISTANT (EU) 230 50 X
73150 32 CENTRIVAP COMPLETE W/ HEAT BOOST (EU) 230 50 X
7315033 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (EU) 230 50 X
7315040 CENTRIVAP COMPLETE (US) 230 60 X
7315041 CENTRIVAP COMPLETE ACID RESISTANT (US) 230 60 X
7315042 CENTRIVAP COMPLETE W/ HEAT BOOST (US) 230 60 X
7315043 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (US) 230 60 X
7315060 CENTRIVAP COMPLETE UK 230 50 X
7315061 CENTRIVAP COMPLETE ACID RESISTANT UK 230 50 X
7315062 CENTRIVAP COMPLETE W/ HEAT BOOST UK 230 50 X
7315063 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (UK) 230 50 X
73150 70 CENTRIVAP COMPLETE (CHINA) 230 50 X
7315071 CENTRIVAP COMPLETE ACID RESISTANT (CHINA) 230 50 X
73150 72 CENTRIVAP COMPLETE W/ HEAT BOOST (CHI NA) 230 50 X
7315073 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (CHINA) 230 50 X
1338000
Power Cord Part # Plug Type
1334500 NEMA 5-15P
1336100 CEE 7/7
1338000 NEMA 6-15P
1332600 BS 1363
1332700 CH1-10P
1332600
1332700
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Page 11
Original instructions
Plus the following:
Part # Component Description
7314000 User’s Manual
7396206 Coupling Insert
7455100 Rotor, 12-13 mm (Standard Models)
7455101 Rotor, 12-13 mm PTFE (Acid Resistant Models)
7450200 Glass Jar
If you do not receive one or more of the components listed for your CentriVap, or
if any of the components are damaged, contact Labconco Corporation
immediately for further instructions.
Setting Up Your CentriVap
After you verify receipt of the proper components, move your CentriVap to the
level and sturdy location where you want to install it. There is no need to secure
the CentriVap to the work surface. Then, follow the steps listed below.
Chapter 3: Getting Started
Emergency Access Into the Chamber
The CentriVap is designed to prevent access to the chamber in the event of a
power disruption. If it is necessary to open the lid when there is no electrical
power connected to the CentriVap, insert a small screwdriver or similar
instrument into the small round hole on the left side of the case near the top
behind the control panel. This will unlock the lid latch mechanism. While
holding the screwdriver in place, raise the lid with the other hand.
CAUTION: Never attempt to defeat the latch or open the lid while the
CentriVap is running. Personnel injury can result from moving parts and
chemicals.
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Original instructions
Chapter 3: Getting Started
Exhaust Port
If the CentriVap is not located in a fume hood, attach one end of a user-supplied
3/8" ID hose to the exhaust port on the back of the unit. Clamp securely. Route
the other end to a fume hood or other laboratory ventilation device.
Electrical Connection
Plug the power cord into the receptacle on the back of the CentriVap Concentrator
and plug the other end into a suitable power receptacle.
Rotor Installation
Place the rotor onto the shaft of the Concentrator. Rotate the rotor slightly to
engage the drive pin in the shaft with the slots in the rotor hub. The top of the
shaft should be in line with the top of the rotor hub.
IMPORTANT: Do not use a rotor if it shows any signs of damage.
Chemical Resistance of CentriVap Components
Your CentriVap Complete is designed to be chemical resistant to most
compounds that are commonly used in the concentration processes that are
performed in it. However, by necessity, the CentriVap is comprised of a number
of different materials, some of which may be attacked and degraded by certain
chemicals. The degree of degradation is obviously dependent on the concentration
and duration of exposure. Some major components of the CentriVap that are
susceptible to degradation are as follows:
Acids Bases Solvents
Acetic Acid 20%
Boric Acid
Formic Acid
Hydrobromic Acid 20%
Hydrochloric Acid 20%
Nitric Acid 20%
Sulfuric Acid 10%
Trifluoroacetic Acid (TFA)
Ammonium Hydroxide
Acetone
Acetonitrile
Chloroform
Dimethyl Formamide
Dimethyl Sulfoxide (DMSO)
Ethanol
Ethyl Acetate
Hexanes
Isoproponal
Methanol
Methylene Chloride
Methyl t-Butyl Ether (MTBE)
Toluene
COMPONENT MATERIAL
Rotor Hub Polypropylene C C
Chamber
Tubing PVC D C D D D D D D C D D D D
Lid Gasket EPDM D D C D
Cold Trap
Gasket
Rotor
Rotor Shaft &
Cold Trap
Bearings
Epoxy coated
Aluminum
Neoprene D D C D D C D D D D D C D
Anodized
Aluminum
Stainless
Steel
High Carbon
Steel
C D C D
D D
D D D
D D D D D D D D D
Water
C-Moderate Degradation-Questionable use
D-Severe Degradation-Infrequent use recommended-immediate thorough cleaning required
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Page 13
Original instructions
When using compounds in the CentriVap that are hostile to the materials of
construction, it is imperative that the equipment is appropriately maintained.
• After each run, clean up all residues, spills and materials that might have
splashed in the chamber using agents suitable for the substance involved.
• Drain the cold trap immediately after the collected ice is melted to prevent
corrosive liquids from residing in the trap. Flush out the trap with water after
draining.
DO NOT chip ice off the cold trap walls as damage may occur.
Solvent Safety Precautions
CAUTION: The CentriVap is not classified as “explosion proof.” It has been
designed with safety as a primary consideration and should be used in a
prudent manner using “good laboratory practices.” It has been designed for
use with compounds as described in the United States National Electrical
Code Class I, Group D. The heater may be programmed to run as hot as
100°C, however, the heater element may normally run at 110°C. A thermal
fuse limits the heater to a maximum temperature of 120°C. It is important
that the solvents used are compatible with these temperatures. Do not
evaporate solvents that have an autoignition temperature below 180°C. Do
not evaporate solvents that are classified as Group A, B, or C by the National
Electrical Code. Evaporate only non-flammable or Group D solvents with
autoignition temperatures 180°C or above. Use of other compounds could
cause an explosion.
Chapter 3: Getting Started
CAUTION: Solvents used in the CentriVap may be flammable or hazardous.
Use extreme caution and keep sources of ignition away from the solvents.
When using flammable or hazardous solvents, the CentriVap should be
operated inside a fume hood.
If a sample is spilled in the chamber it must immediately be cleaned up.
Hazardous materials such as strong acids or bases, radioactive substances
and volatile organics, must be handled carefully and promptly cleaned up if
spilled.
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Page 14
Original instructions
Chapter 3: Getting Started
Do not store flammable or hazardous solvents within 12 inches (300 mm) of
the CentriVap.
IMPORTANT: Various Federal, State or local regulations may govern the
disposal of substances used in connection with this equipment. All users of
this equipment are urged to become familiar with any regulations that apply
in the user’s area concerning the dumping of waste materials in or upon
water, land or air and to comply with such regulations.
10
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Page 15
Original instructions
CChhaapptteerr 44::
UUssiinngg YYoouurr CCeennttrriiVVaapp CCoom
After your CentriVap Complete has been installed as detailed in Chapter 3:
Getting Started, you are ready to begin using your CentriVap. Read this chapter
to learn how to:
• Set operating parameters.
• Operate the controls.
• Properly select and position glassware inside your CentriVap.
• Understand the display.
• Interrupt a cycle after it has begun.
NOTE: See Appendix C: CentriVap Specifications for electrical
requirements.
NOTE: Do not store or stack supplies or equipment on top of the CentriVap.
mpplleettee
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Page 16
Original instructions
Chapter 4: Using Your CentriVap Complete
Planning
Thoroughly understand procedures and the equipment operation prior to
beginning work. The unique performance of the CentriVap is dependent upon the
proper balance of heat, vacuum and centrifugal force. If the proper balance is not
established, it is possible to damage or lose a portion of the sample. Therefore, if
you are unfamiliar with the CentriVap or are attempting a new protocol, it may be
helpful to make a trial run that is void of the sample you are attempting to
concentrate.
Glassware Selection
Sample tubes should be filled no more than half full with substances having a
density no greater than 1400 kg/m3. Select the size of the sample tube so it is
compatible with the rotor and the desired sample size. Tubes should not be loose
in the rotor. Rotors are available with holes for various size tubes. Refer to
Chapter 6: Accessories for your CentriVap Complete for available rotor sizes.
Loading Glassware Into the CentriVap
Smooth operation of the CentriVap is dependent upon proper balance of the
machine. Therefore, if less than a full load of samples is run, it is important to
load samples into the CentriVap in a fairly symmetrical manner distributing the
weight of the samples evenly in the sample rotor.
Temperature Setting Guidelines
The evaporation rate achieved by the CentriVap is dependent upon a variety of
factors. These include the nature of the solvent, the temperature and the pressure
in the vacuum system.
As a general guideline, to speed the evaporation process, the CentriVap
temperature should be set as high as possible as long as the temperature will not
damage the sample or cause the sample to bump.
Time Setting Guidelines
The CentriVap Complete has two timers. The “RUN TIME” turns the entire
concentrator off after the user set period of time. This stops the rotation, turns off
the heater and vacuum pump and bleeds vacuum from the system. The heater
may be turned off prior to the concentrator turning off so heat-sensitive samples
may be protected from exposure to excessive heat after the solvent has
evaporated. To turn the heater off prior to stopping the entire system, enter a set
time into the memory at the “HEATER TIME.” When the programmed time
expires, the heater will turn off but the rotation and vacuum will continue until the
run time expires.
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Page 17
Original instructions
The tables in Appendix C: CentriVap Specifications indicate approximate times
required to evaporate various common solvents. Actual times must be determined
by the user. The CentriVap can be set to alarm after a preset period of operation.
When the time expires, the CentriVap will give an audible alarm and turn itself
off.
Heat Boost Operation
Some CentriVap models are equipped with a secondary “Heat Boost” heater,
which is positioned on the sidewall of the chamber near the top. It provides
additional heat to the samples to speed evaporation. The “Heat Boost” heater is
controlled by the microprocessor to maintain the set point temperature at the
bottom of the chamber to prevent excessive overheating of the samples.
CentriVap Controls
The control panel for the CentriVap is shown below with a description about its
function.
Chapter 4: Using Your CentriVap Complete
1. Display – The liquid crystal display (LCD) shows set point parameters and
actual measured conditions.
2. Program Buttons – Used to initiate the start of a run with the use of just one
button.
3. Run/Stop Button – Used to start or stop a run.
4. Preheat Button – Used to turn on the heater to preheat the chamber prior to
loading samples.
5. Increase Button – When pressed, the last selected set point will increase.
6. Decrease Button – When pressed, the last selected set point will decrease.
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Original instructions
Chapter 4: Using Your CentriVap Complete
7. Set Point Select Button – To select a parameter to change, press the select
button. Arrows on the display will point to the parameter that may be altered.
8. Heat Boost Button – If the model is so equipped, pressing the button will
activate or deactivate the auxiliary Heat Boost heater.
9. Cold Trap Button – Used to start or stop the refrigeration system for the cold
trap.
Operating the CentriVap Controls
Preheat:
To preheat the chamber, press the “PREHEAT” button. The display will show:
SP: XXX ACT: XXX
Press the increase or decrease button until the desired set point (SP) is displayed.
The actual chamber temperature, (ACT) is displayed to the right. When power is
being supplied to the heaters, a bar under the actual temperature will illuminate.
The chamber will continue to preheat until either the “PREHEAT” button is
pressed again or the “RUN” button is pressed.
Select existing program:
Operating parameters can be stored in memory so protocols can be repeated.
Nine programs can be stored. To select a program, press the set point “SELECT”
button until arrows point to the program number indicating that this set point can
be run or altered. To change the program number, press the increase or decrease
button until the desired program number is displayed. When the program number
is changed, all its set points change also to indicate the last entered parameters for
that program.
Store frequently run protocols in program 1, 2, or 3. Then, by pressing “PROG
1,” “PROG 2” or “PROG 3,” the stored program will be initiated without having
to press any other button. Pressing just the one button starts the rotor, the heater,
the timers and the vacuum pump.
Change “Temperature” Set Point:
To change the “Temperature” set point, press the set point “SELECT” button until
arrows point to the “Temperature” set point, which can be changed from - (OFF)
up to 99° by pressing the increase or decrease buttons. The set point is 100°C if
the display shows “HI.” The last entered set point is stored in memory.
Change “Run Time” or “Heater Time” set point:
To change the time set point, press the set point “SELECT” button until arrows
point to the “Heater Time” or “Run Time” set point which can be changed from 1
to 999 minutes by pressing the increase or decrease buttons. If it is desired to
have the CentriVap run continuously without alarming at the end of a time period,
press the increase button until the ”Run Time” display says “ON.” The last
entered set point is stored in memory.
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Page 19
Original instructions
If during a run the “STOP” button is pressed, the timers remember the time at
which stop occurred. If “RUN” is then pressed, the timers continue to count
down from the time at which they were stopped. If you are running program 1,
program 2 or program 3, pressing “PROG 1,” “PROG 2” or “PROG 3” resets the
timers to the original set point time and the CentriVap starts a new run.
To reset the timers to the original set point time when running programs other
than program 1, program 2 or program 3, press the start button and hold it for five
seconds. The display will indicate that the timer is reset.
Operating the CentriVap
CAUTION: To avoid personnel injury; Do not operate the CentriVap if the
lid is scratched or nicked, or shows signs of damage. A damaged lid could fail
under vacuum.
Chapter 4: Using Your CentriVap Complete
While the CentriVap is operating, do not lean on the lid, do not stand near it
longer than necessary and do not place hazardous materials within 12 inches.
1. Turn “ON” the CentriVap power switch.
2. Press the Cold Trap “ON” switch. The “Y” in the display under the cold trap
will flash. When the trap reaches operating temperature the “Y” will
illuminate steady.
3. Select a program or set the set point parameters.
4. Preheat the chamber if desired.
5. Turn on Heat Boost if the CentriVap is so equipped and additional heat is
desired.
6. Place samples in vials. Normally the vials should be no more than half full.
Place vials in a rotor.
7. Load the rotor with samples into the chamber.
8. Close the lid.
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Original instructions
Chapter 4: Using Your CentriVap Complete
9. Press “RUN.” If the display was showing set point parameters, it will change
to show actual parameters. Press “RUN” again. The “S” (STOP) in the
display changes to “R” (RUN). The program on the display alternately
displays “R” or the number of the program that is running. If you intend to
run program 1, 2 or 3, simply press “PROG 1,” “PROG 2” or “PROG 3” to
quick start the CentriVap. The rotor will start, the vacuum break valve will
close and the vacuum pump will start after the rotor reaches operating speed.
10. Set point parameters can be altered at any time during a run by first selecting
the parameter using the set point “SELECT” button and then pressing the
“INCREASE” or “DECREASE” switch.
11. If the run time set point is used, at the end of the set time an alarm sounds. All
functions cease.
12. Press “STOP” to terminate operation if the CentriVap has not already stopped
itself.
13. When the evaporation is complete, allow the rotor to stop moving, lift the lid
and remove the samples.
CentriZap™ Strobe Light
The CentriZap will enable you to see the samples as they are rotating in the rotor.
To use the strobe light while the CentriVap is operating, remove the light from its
holder on the front of the unit, press the trigger and shine the light on the samples
in the rotor.
Operational Notes
As solvent is evaporated in the Concentrator and then condensed in the Cold Trap,
it is normal for the Cold Trap temperature to rise as its load increases.
Depending on which solvent is used, the volume of the sample and the system
operating parameters, the Cold Trap may warm up sufficiently to cause the
“READY” indicator to turn off. As the sample reaches completion, the load on
the Cold Trap will decrease and its temperature will decrease. The “READY”
indicator will once again illuminate.
When the CentriVap is turned on, the CentriVap returns to the same mode
(“RUN” or “STOP”) that it was in when the power was turned off. If the
CentriVap was in the “RUN” mode when the power was turned off, when the
power is turned on, the CentriVap attempts to return to the programmed set points
and continue the run.
To reset the timers to the original set point when running programs other than
program 1, program 2 or program 3, press the start button and hold it for five
seconds. The display will indicate that the timer is reset.
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Original instructions
Interrupting a Cycle After it Has Begun
At any time during a run, the cycle may be stopped by pressing the “STOP”
button. This shuts off all operating functions. After the rotor stops, the lid may
be opened. If it is necessary to re-start the CentriVap, close the lid and press
“RUN.” The CentriVap resumes operation at the same set point parameters and
the timer continues to count down from the time at which the CentriVap was
stopped.
Gas Ballast
The vacuum pump has a gas ballast that can be used to reduce condensation of
vapors in the pump and exhaust line. This is most likely to occur if the Cold Trap
is not running in conjunction with the evaporation process. The gas ballast is
OPEN if the arrow on the gas ballast cap is pointing “IN” towards the CentriVap.
The gas ballast is CLOSED if the cap is rotated 180°, so that the arrow is pointing
“OUT” of the CentriVap.
Chapter 4: Using Your CentriVap Complete
Safety Precautions
Special precautions must be observed if the materials used in the CentriVap
Concentrator are known to be hazardous, toxic, radioactive, or contaminated with
pathogenic micro organisms. These actions should include but are not limited to
the following:
• Refer to the World Health Organization Laboratory Biosafety Manual,
paying special attention to information about centrifuges and the handling
of hazardous materials.
• Operate or vent the CentriVap Concentrator inside a suitable fume hood or
ventilation device. Load rotors in a ventilation device.
• Periodically inspect all parts of the CentriVap Concentrator including the
lid, gasket, chamber, plumbing components and rotors.
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Original instructions
CChhaapptteerr 55::
MMaaiinnttaaiinniinngg YYoouurr CCeennttrriiVVaapp
CCoom
Under normal operation, the CentriVap Complete requires little maintenance.
The following maintenance schedule is recommended. Before servicing the
CentriVap, disconnect electrical power. Special precautions must be observed if
materials used in the CentriVap are known to be hazardous, toxic, radioactive or
contaminated with pathogenic micro organisms. Before servicing, the CentriVap
must be suitably decontaminated. Wear appropriate eyewear, gloves and other
safety apparel.
As needed:
Before using any cleaning or decontamination method except those recommended
by the manufacturer, users should check with the manufacturer that the proposed
method will not damage equipment.
1. Clean up all spills; remove liquids from the chamber. Clean or decontaminate
2. Clean lid and gasket using soft cloth, sponge or chamois and a mild, non-
mpplleettee
all surfaces using agents suitable for the substance spilled.
abrasive soap or detergent.
3. Utilization of acids requires immediate cleaning and neutralization after a run
or physical damage to the stainless steel collection chamber will result.
4. Check the Cold Trap for condensed or frozen solvents and dispose of
appropriately. Completely empty the trap before the next run.
5. If the media in the optional clear canister has changed color, discard and
replace the insert with a new insert. For the radiochemical trap insert, no
indicator exists; therefore, it should be discarded after each use. In radioactive
applications, the system should be monitored with a Geiger counter.
6. Check rotors for loose or missing parts. Tighten or replace as required. Do
not use defective rotors.
7. Inspect the chamber to insure that there are not cracks or structural damage.
Call Labconco if defects exist.
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Original instructions
8. Check continuity of the protective earth between the ground terminal on the
power inlet and a bare metal housing panel. Contact Labconco if there is no
continuity.
9. Repair any defects to the surface where the CentriVap is installed.
Monthly:
1. The rubber components on the CentriVap may eventually deteriorate and
require replacement. The effective life of rubber parts depends upon both
their usage and the surrounding environment. Check all rubber hoses and
gaskets and replace any that show signs of hardening, permanent set or
deterioration.
2. Using a soft cloth, sponge or chamois and a mild, non-abrasive soap or
detergent, clean the glass lid.
3. Using a soft cloth, sponge, or chamois and a mild, non-abrasive soap or
detergent, clean the exterior surfaces of the unit. Liquid spray cleaners and
polishes may be used on the exterior surfaces. Do not use solvents to remove
stains from the exterior surfaces as they may damage the finish.
Chapter 5: Maintaining Your CentriVap Complete
Annually:
1. Every 12 months, or more often, if the Cold Trap is operated in a dusty
environment, the refrigeration system condenser of the Cold Trap should be
cleaned. Using a vacuum cleaner with brush attachment, clean the condenser
to ensure proper airflow for peak performance. Disconnect power before
removing covers.
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Original instructions
CChhaapptteerr 66::
AAcccceessssoorriieess ffoorr YYoouurr CCeennttrriiVVaapp
CCoom
The configuration of your CentriVap Complete can be changed to accommodate
your needs. Read this chapter to learn how to:
mpplleettee
• Install a vacuum gauge.
• Install a secondary chemical trap.
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Original instructions
The following accessories are available for the CentriVap Complete.
PART # DESCRIPTION
7462900
7462901*
7450700
7450701*
7455000
7455001*
7455100
7455101*
7455200
7455201*
7553100
7553101*
7553000
7553001*
Chapter 6: Accessories for Your CentriVap Complete
Rotor (DNA)
Holds (72) 0.5 ml microcentrifuge tubes
and
(60) 1.5 ml microcentrifuge tubes or
(60) 2.0 ml microcentrifuge tubes
Rotor (1.5 ml)
Holds (132) 1.5 ml microcentrifuge tubes or
(132) 2.0 ml microcentrifuge tubes
Rotor (15 ml)
Holds (44) 12 x 55 mm tubes or
(36) 12 x 75 mm tubes or
(36) 12 x 95 mm tubes or
(36) 13 x 75 mm tubes or
(36) 13 x 100 mm tubes
and
(24) 16 x 100 mm tubes or
(18) 16 x 120 mm conical tubes or
(18) 17 x 95 mm tubes or
(18) 17 x 100 mm centrifuge tubes or
(18) 17 x 120 mm tubes
Rotor (12-13 mm)
Holds (40) 1.5 ml microcentrifuge tubes or
(40) 2.0 ml microcentrifuge tubes
and
(16) 12 x 55 mm tubes or
(100) 12 x 75 mm tubes or
(64) 12 x 95 mm tubes or
(100) 13 x 75 mm tubes or
(64) 13 x 100 mm tubes
Rotor (50 ml)
Holds (32) 1.5 ml microcentrifuge tubes or
(32) 2.0 ml microcentrifuge tubes
and
(12) 28 x 115 mm conical tubes or
(12) 28 x 135 mm conical tubes or
(12) 28 x 140 mm conical tubes
Rotor (100 ml pear shaped flask)
Holds (8) 100 ml flasks
Rotor (6 microtiter)
Holds (6) Standard 96 well plates or
(3) Deep well plates
*PTFE Coated
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Chapter 6: Accessories for Your CentriVap Complete
PART # DESCRIPTION
Original instructions
7461900
7461901*
7460900 Clear Canister – Accommodates inserts listed below
7814800 Acid Trap Insert
7814900 Moisture Trap Insert
7995600 Ammonia Trap Insert
7815000 Radiochemical Trap Insert
7815200 Solvent Trap Insert
Rotor (96 well plate)
Holds (4) Standard 96 well plates or
(2) Deep well 96 well plates
Installing a Vacuum Gauge
A user-supplied vacuum gauge may be attached to the CentriVap to monitor the
vacuum level. Attach the vacuum gauge to the barb end of the Quick Disconnect
Coupling fitting that was supplied with the CentriVap using a length of suitable
rubber hose. The Quick Disconnect Coupling fitting can then be pushed into the
mating connector on the rear left side of the CentriVap. The Quick Disconnect
Coupling fitting can be removed from the mating connector by pressing on the tab
on the connector and then pulling out the Quick Disconnect Coupling fitting.
Always remove the Quick Disconnect Coupling from the CentriVap when a
vacuum gauge is not attached to it.
22
USER SUPPLIED
VACUUM TUBING
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Original instructions
Installing a Secondary Chemical Trap
An accessory secondary chemical trap is available to minimize the exhausting of
solvents into the atmosphere. Attach the bracket to the right hand side of the
housing using the screws provided. Attach the hose from the outlet on the
vacuum pump to the “out” connector of the canister housing. Unscrew the clear
blow of the canister housing from the head. Remove both the upper and lower
caps from the filter cartridge and insert the small end of the cartridge into the hole
in the center of the head. Reinstall the clear bowl.
Chapter 6: Accessories for Your CentriVap Complete
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Original instructions
CChhaapptteerr 77::
TTrroouubblleesshhoooottiinngg
Refer to the following if your CentriVap fails to operate properly. If the
suggested corrective actions do not solve your problem, contact Labconco for
additional assistance. The following failure codes may appear on the display
when problems are sensed by the internal self check routine.
CAUTION: Disconnect power before corrective action is taken.
DISPLAY ERROR CODE CAUSE CORRECTIVE ACTION
Heat Sensor
Close Lid
Latch Fail
Mem Fail P1
MOTOR ERROR
Sensor failure Replace sensor assembly.
Connection failure Repair connection.
Lid open Close lid.
Solenoid failure Check component.
Switch or sensor
failure
Memory failure Push program button #1.
New memory IC chip Push program button #1.
Bad memory IC chip Call Labconco – Replace IC chip or
Defective motor Replace motor.
Hall effect sensor
failure
Wire failure Replace wire.
Check connections.
control PCB.
Replace sensor.
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Original instructions
Chapter 7: Troubleshooting
Other corrective actions for potential problems are as follows:
PROBLEM CAUSE CORRECTIVE ACTION
Unit will not operate
Excessive vibration
Sample odor in lab
Evaporation rate is
Unit not connected to
electrical power
Circuit breaker blown Correct electrical problem and reset circuit
Lid open Close lid.
Sample tubes not located
symmetrically in rotor
Vent hose exhausting into
lab area
Heater inoperable Contact Labconco.
Connect unit to proper electrical
receptacle.
breaker by pressing button.
Reposition sample tubes.
Redirect hose to fume hood.
reduced
No vacuum/poor vacuum
Vacuum pump failure Check pump.
Obstruction in hose Remove obstruction or replace hose.
Lack of adequate vacuum See below.
Pump not on Turn on pump.
Control valve open Check control valve.
Leaks in lines or
Locate and repair.
connectors or gasket
Foreign material on lid
Clean gasket and lid.
gasket
Pump is not functioning
properly
Check pump by locating vacuum gauge
closer to pump and close off rest of system.
If pump is faulty, seek authorized service
or replace pump.
Cold Trap or
Concentrator gasket is
Check gasket for cleanliness. Adjust
gasket as needed.
not sealing properly
Cold Trap lid not seated Hold lid down until vacuum is initiated.
Ice formed on Cold
Defrost and wipe dry.
Trap lid sealing surface
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Original instructions
Chapter 7: Troubleshooting
PROBLEM CAUSE CORRECTIVE ACTION
Recovery of condensate in
Cold Trap is less than
normally expected
Cold Trap is not ON Check to make sure switch is ON and
condensing unit fan is moving air out
of the sides of the cabinet.
Cold Trap does not cool
down
Turn vacuum pump OFF and allow
Cold Trap to cool for at least 30 min. to
reach temperature.
Unit starts and shuts off
-50°C temperature can be checked with
a solvent thermometer or digital
thermometer.
Latch optical sensor
improperly calibrated
Unplug power cord. Wait 10 seconds.
Plug in power cord.
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Original instructions
AAppppeennddiixx AA::
CCeennttrriiVVaapp CCoom
The following pages list components that are available for your CentriVap
Complete. The parts shown are the most common replacement parts. If other
parts are required, contact Product Service.
mppoonneennttss
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Appendix A: CentriVap Components
Original instructions
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Original instructions
AAppppeennddiixx BB::
CCeennttrriiVVaapp DDiim
meennssiioonnss
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Original instructions
AAppppeennddiixx CC
CCeennttrriiVVaapp SSppeecciiffiiccaattiioonnss
This Appendix contains technical information about the CentriVap including
specifications, environmental operating conditions and evaporation rates.
Mechanical Specifications
• Weight: 145 lbs. (66 kg)
• Dimensions: See Appendix B
Electrical Specifications
• Nominal amperage for 115V/60 Hz CentriVap: 12A.
• Nominal amperage for 230V/50Hz CentriVap: 6.5A.
• Nominal amperage for 230V/60Hz CentriVap: 6.5A.
• Phase: Single
• Heater Power: 300 watts – Main heater
• Heater Power: 300 watts – Heat boost
• Rotor Speed: Up to 1,725 RPM
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Original instructions
Environmental Conditions
• Indoor use only.
• Maximum altitude: 6562 feet (2000 meters).
• Ambient temperature range: 41° to 104°F (5° to 40°C).
• Maximum relative humidity: 80% for temperatures up to 88°F (31°C),
• Main supply voltage fluctuations not to exceed ±10% of the nominal
• Transient overvoltages according to Installation Categories II
Appendix C: CentriVap Specifications
decreasing linearly to 50% relative humidity at 104°F (40°C).
voltage.
(Overvoltage Categories per IEC 1010). Temporary voltage spikes on the
AC input line that may be as high as 1500V for 115V models and 2500V
for 230V models are allowed.
• Used in an environment of Pollution degrees 2 (i.e., where normally only
non-conductive atmospheres are present). Occasionally, however, a
temporary conductivity caused by condensation must be expected, in
accordance with IEC 664.
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Appendix C: CentriVap Specifications
Evaporation Rates
Methylene Chloride
Tube Size
(ml)
50 12 25 45 off Diaphragm 93 yes 3.23
50 12 25 45 on Diaphragm 89 yes 3.37
15 18 10 45 off Diaphragm 57 yes 3.16
15 18 10 45 on Diaphragm 55 yes 3.27
Toluene
50 12 25 45 off Diaphragm 213 yes 1.41
50 12 25 45 on Diaphragm 151 yes 1.99
50 12 25 100 off Diaphragm 63 yes
50 12 25 100 on Diaphragm 57 yes
15 18 10 45 off Diaphragm 93 yes
15 18 10 45 on Diaphragm 85 yes
15 18 10 100 off Diaphragm 42 yes
15 18 10 100 on Diaphragm 37 yes
Acetonitrile
50 12 25 45 off Diaphragm 182 yes 1.65
50 12 25 45 on Diaphragm 178 yes
50 12 25 100 off Diaphragm 97 yes
50 12 25 100 on Diaphragm 89 yes
15 18 10 45 off Diaphragm 117 yes
15 18 10 100 off Diaphragm 63 yes
15 18 10 100 on Diaphragm 53 yes
1.5 132 1 45 off Diaphragm 57 yes
1.5 132 1 60 off Diaphragm 44 yes
1.5 132 1 75 off Diaphragm 37 yes
1.5 132 1 100 off Diaphragm 27 yes
1.5 132 1 100 on Diaphragm 25 yes
Methanol
50 12 25 45 off Diaphragm 245 yes 1.22
15 18 10 45 off Diaphragm 145 yes 1.24
1.5 132 1 45 off Diaphragm 75 yes
1.5 132 1 60 off Diaphragm 58 yes
1.5 132 1 75 off Diaphragm 51 yes
1.5 132 1 100 off Diaphragm 38 yes
1.5 132 1 100 on Diaphragm 34 yes
Number
of
Samples
bp 40
Sample
Size (ml)
bp 111
Heater
Temp
(C)
Heat
Boost
Vacuum
Pump
Time to
Dry
(min)
Cold
Trap
Overall
(ml/min)
4.76
5.26
1.94
2.12
4.29
4.86
bp 82
1.69
3.09
3.37
1.54
2.86
3.40
2.32
3.00
3.57
4.89
5.28
bp 65
1.76
2.28
2.59
3.47
3.88
Rate
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Water
Tube Size
(ml)
50 12 25 45 off Diaphragm 1120 yes 0.27
50 12 25 100 off Diaphragm 416 yes 0.72
50 12 25 100 on Diaphragm 383 yes
15 18 10 45 off Diaphragm 618 yes
1.5 132 1 45 off Diaphragm 358 yes
1.5 132 1 60 off Diaphragm 251 yes
1.5 132 1 75 off Diaphragm 193 yes
1.5 132 1 100 off Diaphragm 137 yes
1.5 132 1 100 on Diaphragm 122 yes
Vacuum Pump – Rated at 7 mbar ultimate vacuum and 38 L/min displacement
Chamber preheated prior to each run to run temp.
Number
of
Samples
Appendix C: CentriVap Specifications
bp 100
Sample
Size (ml)
Heater
Temp
(C)
Heat
Boost
Vacuum
Pump
Time to
Dry
(min)
Cold
Trap
Rate
Overall
(ml/min)
0.78
0.29
0.37
0.53
0.68
0.96
1.08
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Original instructions
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