Labconco CentriVa DNA Systems User Manual
CentriVap®DNA Systems
Models
7970010
7970011
7970030
7970035
7970037
Uss
U
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Labconco Corporation
8811 Prospect Avenue
Kansas City, MO 64132-2696
800-821-5525, 816-333-8811
FAX 816-363-0130
E-MAIL labconco@labconco.com
HOME PAGE www.labconco.com
Please read the User’s Manual before operating equipment.
Copyright © 2009, 2012, 2013 Labconco Corporation. All rights reserved.
The information contained in this manual and the accompanying products are copyrighted and all rights
reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design
changes without obligation to notify any person or entity of such change.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.
The warranty for all Labconco products will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner, except the following;
• Purifier® Logic® Biological Safety Cabinets and PuriCare® Lab Animal Research Stations
carry a three-year warranty from date of installation or four years from date of shipment from
Labconco, whichever is sooner.
• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from date
of installation or three years from date of shipment from Labconco, whichever is sooner.
• Blood Drawing Chairs carry a ten year warranty.
• Carts carry a lifetime warranty.
• Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or
replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement may
be altered by a specific published amendment. No individual has authorization to alter the provisions
of this warranty policy or its amendments. Lamps and filters are not covered by this warranty.
Damage due to corrosion or accidental breakage is not covered.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier.
Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525
or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #7397609, Rev. B
ECO H435
T
AABBLLEE
T
CHAPTER 1: INTRODUCTION 1
Safety Symbols 2
CHAPTER 2: PREREQUISITES 3
Electrical Requirements 3
Location and Exhaust Requirements 4
Space Requirements 4
CHAPTER 3: GETTING STARTED 5
Unpacking Your CentriVap 6
CentriVap Components 6
Setting Up Your CentriVap 7
Emergency Access Into the Chamber 7
Exhaust Port 7
Electrical Connection 7
Rotor Installation 7
Chemical Resistance of CentriVap Components 8
Solvent Safety Precautions 9
CHAPTER 4: USING YOUR CENTRIVAP 11
Planning 12
Glassware Selection 12
Loading Glassware Into the CentriVap 12
Temperature Setting Guidelines 12
Time Setting Guidelines 12
CentriVap Controls 13
Operating the CentriVap Controls 14
Operating the CentriVap 15
Operational Notes 16
Interrupting a Cycle After it Has Begun 16
Gas Ballast 16
Safety Precautions 17
CHAPTER 5: MAINTAINING YOUR CENTRIVAP 18
O
O
FF
C
C
OONNTTEENNTTSS
CHAPTER 6: ACCESSORIES FOR YOUR CENTRIVAP 20
Installing a Glass Lid 22
Installing a Secondary Chemical Trap 22
Installing a Vacuum Gauge 23
Installing a CentriZap Strobe Light 24
CHAPTER 7: TROUBLESHOOTING 25
APPENDIX A: CENTRIVAP COMPONENTS 27
APPENDIX B: CENTRIVAP DIMENSIONS 29
APPENDIX C: CENTRIVAP SPECIFICATIONS 30
Electrical Specifications 30
Environmental Conditions 30
Evaporation Rates 31
DECLARATION OF CONFORMITY 32
CChhaapptteerr 11::
IInnttrroodduuccttiioonn
Congratulations on your purchase of a Labconco CentriVap DNA Centrifugal
Concentrator . Models are available for operation on 115V or 230V.
The CentriVap Concentrator uses centrifugal force with heat and vacuum to
rapidly dry precipitates of DNA and RNA samples. Centrifugation eliminates
bumping and foaming as vacuum is applied and also concentrates the solute in the
bottom of the vial. This allows recovery of solutes from sample volumes as small
as a few microliters. The concentrator is equipped with a 300 watt heater to speed
evaporation by warming the chamber during processing. A timed outlet for the
vacuum pump connection delays the start of the pump until the rotor reaches
operating speed. A safety switch prevents the concentrator from starting when the
lid is in the open position and a latch prevents the lid from being opened while the
rotor is turning. The compact, low profile design allows the concentrator to be
placed on a bench top or other laboratory work surface.
All wetted parts within the pump are PTFE, or other chemical resistant
fluoropolymers. A glass trap is provided to remove liquid from the system before
it enters the pump and a second glass trap is provided on the outlet side of the
pump to trap liquid expelled from the pump.
The microprocessor, which controls the operation of the heater and the motor,
provides excellent regulation and reproducibility of protocols. A manually preset
timer can signal the end of the run. Nine different protocols can be stored in
memory so exact test parameters can be easily repeated.
Product Service 1-800-522-7658
1
Chapter 1: Introduction
Safety Symbols
Your CentriVap Concentrator was designed with safety in mind, however
conditions may exist that could be hazardous.
Throughout this manual potentially hazardous conditions are identified using the
following words and symbols.
It is important that you understand the warnings listed throughout this manual
before you operate the CentriVap Concentrator.
2
Product Service 1-800-522-7658
CChhaapptteerr 22::
PPrreerreeqquuiissiitteess
Before you install your CentriVap, you need to prepare your site for installation.
Carefully examine the location where you intend to install your CentriVap. You
must be certain that the area is level and of solid construction. In addition, an
exhaust means must be provided. An electrical source must be located near the
installation site.
Carefully read this chapter to learn:
• The electrical supply requirements.
• The exhaust requirements.
Refer to Appendix C: CentriVap Specifications for complete CentriVap electrical
and environmental conditions, specifications and requirements.
Electrical Requirements
The CentriVap Concentrator requires a dedicated grounded electrical outlet. This
outlet requires a 15 Amp circuit breaker or fuse for models rated at 115V (60 Hz).
An 8 Amp circuit breaker or fuse is required for models rated at 230V (50/60 Hz).
Various models are available for use in different locations throughout the world.
Each has a unique plug suitable for the specific location. See CentriVap
Components in Chapter 3 for detail plug specifications. If the plug supplied with
the CentriVap Does not match with the available receptacle, remove this plug and
replace it with an approved plug of the suitable style.
It is recommended that an emergency switch for disconnecting the mains in the
case of a malfunction be located remote from the CentriVap, preferably outside
the room in which the CentriVap is housed, or adjacent to the exit from that room.
Product Service 1-800-522-7658
3
Chapter 2: Prerequisites
Location and Exhaust Requirements
The CentriVap Concentrator should be located on a surface that is stable, flat and
level.
WARNING: The CentriVap System should be located within a fume hood if
hazardous or flammable solvents are used. Heating of materials could lead
to the liberation of hazardous gases. In all cases, regardless of the solvent
used, it is strongly recommended that the vacuum pump is vented in a fume
hood. An accessory secondary trap is available to minimize the exhausting of
solvents into the atmosphere. This does not, however, negate the need to
exhaust the vacuum pump into a fume hood. Failure to properly vent the
CentriVap will expose personnel to potentially harmful chemicals.
The CentriVap has not been evaluated by an approval agency for the use of
biological, radio toxins or flammable liquids or materials.
Space Requirements
Refer to Appendix B: CentriVap Dimensions for dimensional drawings of the
CentriVap DNA System.
No person or any hazardous material should be within 12 inches of the CentriVap
Concentrator while it is operating.
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Product Service 1-800-522-7658
CChhaapptteerr 33::
GGeettttiinngg SSttaarrtteedd
Now that the site for your CentriVap is properly prepared, you are ready to
unpack, inspect, install, and test your CentriVap. Read this chapter to learn how
to:
• Unpack and move your CentriVap.
• Set up your CentriVap.
• Connect the electrical supply source to your CentriVap.
• Properly exhaust your CentriVap.
• Safely use solvents with your CentriVap.
CAUTION: The CentriVap Concentrator weighs over 80 lbs. (36 Kg). The
carton allows for lifting with a mechanical lift truck or hand truck. If you
must lift the CentriVap manually, use at least two (2) persons and follow safe
lifting guidelines.
Product Service 1-800-522-7658
5
Chapter 3: Getting Started
Unpacking Your CentriVap
Carefully unpack your CentriVap and inspect it for damage that may have
occurred in transit. If your CentriVap is damaged, notify the delivery carrier
immediately and retain the entire shipment intact for inspection by the carrier.
The United States Interstate Commerce Commission rules require that claims be
filed with the delivery carrier within fifteen (15) days of delivery.
NOTE: Do not return goods without the prior authorization of Labconco.
Unauthorized returns will not be accepted. If your CentriVap was damaged
in transit, you must file a claim directly with the freight carrier. Labconco
Corporation and its dealers are not responsible for shipping damage.
Do not discard the carton or packing material for your CentriVap until you have
checked all of the components and installed and tested the CentriVap.
CentriVap Components
As previously mentioned, the CentriVap DNA System is available in 115V or
230V. Locate the model of CentriVap you received in the following table. Verify
that the components listed are present and undamaged.
Catalog # Product Description
7970010 CentriVap DNA Concentrator – 115V – USA
1334500 Power Cord with NEMA 5-15P Plug
7462900 Rotor
7397609 User’s Manual
7970011 CentriVap DNA Concentrator – 230V – USA
1338000 Power Cord with NEMA 6-15P Plug
7462900 Rotor
7397609 User’s Manual
7970030 CentriVap DNA Concentrator – 230V – EU
1336100 Power Cord with CEE 7/7 Plug
7462900 Rotor
7397609 User’s Manual
7970035 CentriVap DNA Concentrator – 230V – UK
1332600 Power Cord with BS 1363 Plug
7462900 Rotor
7397609 User’s Manual
7970037 CentriVap DNA Concentrator – 230V – China
1332700 Power Cord with CHI-10P Plug
7462900 Rotor
7397609 User’s Manual
If you do not receive one or more of the components listed for your CentriVap, or
if any of the components are damaged, contact Labconco Corporation
immediately for further instructions.
6
Product Service 1-800-522-7658
Chapter 3: Getting Started
Setting Up Your CentriVap
After you verify receipt of the proper components, move your CentriVap to the
level and sturdy location where you want to install it. There is no need to secure
the CentriVap DNA Concentrator to the work surface. Then, follow the steps
listed below.
Emergency Access Into the Chamber
The CentriVap is designed to prevent access to the chamber in the event of a
power disruption. If it is necessary to open the lid when there is no electrical
power connected to the CentriVap, insert a small screwdriver or similar
instrument into the small round hole on the left side of the case near the top
behind the control panel. This will unlock the lid latch mechanism. While
holding the screwdriver in place, raise the lid with the other hand.
CAUTION: Never attempt to defeat the latch or open the lid while the
CentriVap is running. Personnel injury can result from moving parts and
chemicals.
Exhaust Port
It the CentriVap is not located in a fume hood, attach one end of a .50 inch ID
hose to the exhaust port on the left side on the vacuum pump glass trap exhaust
port. Clamp securely. Route the other end to a fume hood or other laboratory
ventilation device.
Electrical Connection
Plug the power cord into the receptacle on the back of the CentriVap Concentrator
and plug the other end into a suitable power receptacle
Rotor Installation
Place the rotor onto the shaft of the Concentrator. Rotate the rotor slightly to
engage the drive pin in the shaft with the slots in the rotor hub. The top of the
shaft should be in line with the top of the rotor hub.
IMPORTANT: Do not use a rotor if it shows any signs of damage.
Product Service 1-800-522-7658
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