Labconco 7315021, 7315022, 7315020, 7315023 Instruction Manual

Original instructions
CentriVap Complete™
73150 Series
Original instructions
Warranty
Labconco Corporation provides a warranty to the original buyer for the repair or replacement of parts and reasonable labor as a result of normal and proper use of the equipment with compatible chemicals. Broken glassware and maintenance items, such as filters, gaskets, light bulbs, finishes and lubrication are not warranted. Excluded from warranty are products with improper installation, erratic electrical or utility supply, unauthorized repair and products used with incompatible chemicals.
The warranty for the CentriVap® Complete™ will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner. Warranty is non-transferable and only applies to the owner (organization) of record.
Buyer is exclusively responsible for the set-up, installation, verification, decontamination or calibration of equipment. This limited warranty covers parts and labor, but not transportation and insurance charges. If the failure is determined to be covered under this warranty, the dealer or Labconco Corporation will authorize repair or replacement of all defective parts to restore the unit to operation. Repairs may be completed by 3rd party service agents approved by Labconco Corporation. Labconco Corporation reserves the rights to limit this
warranty based on a service agent’s travel, working hours, the site’s entry restrictions and unobstructed access
to serviceable components of the product.
Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This warranty is exclusive and in lieu of all other warranties whether oral, or implied.
Copyright © 2017 Labconco Corporation. The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of this equipment are required to become familiar with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525 or 1-816-333-8811, between the hours of 7:30 a.m. and 5:30 p.m., Central Standard Time.
Part #7314000, Rev. C
ECO K940
Original instructions
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CHAPTER 1: INTRODUCTION 1
Safety Symbols 2
CHAPTER 2: PREREQUISITES 3
Electrical Requirements 3 Location and Exhaust Requirements 4 Space Requirements 4
CHAPTER 3: GETTING STARTED 5
Unpacking Your CentriVap 6 CentriVap Components 6
Setting Up Your CentriVap 7 Emergency Access Into the Chamber 7 Exhaust Port 8 Electrical Connection 8 Rotor Installation 8 Chemical Resistance of CentriVap Components 8 Solvent Safety Precautions 10
CHAPTER 4: USING YOUR CENTRIVAP COMPLETE 11
Planning 12
Glassware Selection 12
Loading Glassware Into the CentriVap 12
Temperature Setting Guidelines 12
Time Setting Guidelines 12
Heat Boost Operation 13
CentriVap Controls 13
Operating the CentriVap Controls 14
Operating The CentriVap 15
CentriZap Strobe Light 16
Operational Notes 16
Interrupting a Cycle After it Has Begun 17
Gas Ballast 17
Safety Precautions 17
Original instructions
CHAPTER 5: MAINTAINING YOUR CENTRIVAP COMPLETE 18
CHAPTER 6: ACCESSORIES FOR YOUR CENTRIVAP
COMPLETE 20
Installing a Vacuum Gauge 22 Installing a Secondary Chemical Trap 23
CHAPTER 7: TROUBLESHOOTING 24
APPENDIX A: CENTRIVAP COMPONENTS 27
APPENDIX B: CENTRIVAP DIMENSIONS 29
APPENDIX C: CENTRIVAP SPECIFICATIONS 30
Electrical Specifications 30 Environmental Conditions 31 Evaporation Rates 32
1
Original instructions
CChhaapptteerr 11:: IInnttrroodduuccttiioonn
Congratulations on your purchase of a Labconco CentriVap Complete. Models are available for operation on 115V or 230V.
The CentriVap Complete combines a concentrator, cold trap, and vacuum pump in one cabinet. The concentrator uses centrifugal force with heat and vacuum to rapidly evaporate and condense solvents from biological and analytical samples. Centrifugation eliminates bumping and foaming as vacuum is applied and also concentrates the solute in the bottom of the vial. This allows recovery of solutes from sample volumes as small as a few microliters. The concentrator is equipped with a 300 watt heater to speed evaporation by warming the chamber during processing. The microprocessor delays the start of the vacuum pump until the rotor reaches operating speed. A safety switch prevents the concentrator from starting when the lid is in the open position and a latch prevents the lid from being opened while the rotor is turning.
The microprocessor, which controls the operation of the heater and the motor, provides excellent regulation and reproducibility of protocols. The end of the run can be signaled by a manually preset timer. Nine different protocols can be stored in memory so exact test parameters can be easily repeated.
As an integral part at the CentriVap Complete, the cold trap can be used to trap moisture, vapors and corrosive fumes as they evaporate from the samples. Standard models can be used for aqueous and organic applications. Acid resistant models are recommended for corrosive applications. The 230V models comply with CE regulations.
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Chapter 1: Introduction
2
Original instructions
Safety Symbols
Your CentriVap Complete was designed with safety in mind, however conditions may exist that could be hazardous.
Throughout this manual potentially hazardous conditions are identified using the following words and symbols.
It is important that you understand the warnings listed throughout this manual before you operate the CentriVap Complete.
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3
Original instructions
CChhaapptteerr 22:: PPrreerreeqquuiissiitteess
Before you install your CentriVap, you need to prepare your site for installation. Carefully examine the location where you intend to install your CentriVap. You must be certain that the area is level and of solid construction. In addition, an exhaust means must be provided. An electrical source must be located near the installation site.
Carefully read this chapter to learn:
The electrical supply requirements.
The exhaust requirements.
The vacuum pump requirements.
Refer to Appendix C: CentriVap Specifications for electrical and environmental conditions, specifications and requirements.
If the unit is not operated as specified in this manual it may impair the protection provided by the unit.
Electrical Requirements
The CentriVap Complete requires a dedicated grounded electrical outlet. This outlet requires a 15 Amp circuit breaker or fuse for models rated at 115V (60 Hz). An 8 Amp circuit breaker or fuse is required for models rated at 230V (50/60 Hz). Various models are available for use in different locations throughout the world. Each has a unique plug suitable for the specific location. See CentriVap Components in Chapter 3 for detailed plug specifications. If the plug supplied with the CentriVap does not match with the available receptacle, remove this plug and replace it with an approved plug of the suitable style.
It is recommend that an emergency switch for disconnecting the mains in the case of a malfunction be located remote from the CentriVap, preferably outside the room in which the CentriVap is housed, or adjacent to the exit from that room.
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Chapter 2: Prerequisites
4
Original instructions
!
Do not position the CentriVap so that it is difficult to remove the power cord from the unit or to operate the power switch.
Do not use any detachable power cord that is not adequately rated for the unit.
Location and Exhaust Requirements
The CentriVap Complete should be located on a surface that is stable, flat and level.
WARNING: The CentriVap should be located within a fume hood if hazardous or flammable solvents are used. Heating of materials could lead to the liberation of hazardous gases. In all cases, regardless of the solvent used, it is strongly recommended that the vacuum pump is vented in a fume hood. An accessory secondary trap is available to minimize the exhausting of solvents into the atmosphere. This does not, however, negate the need to exhaust the vacuum pump into a fume hood. Failure to properly vent the CentriVap will expose personnel to potentially harmful chemicals.
The CentriVap has not been evaluated by an approval agency for the use of biological, radio toxins or flammable liquids or materials.
Space Requirements
Refer to Appendix C: CentriVap Specifications for dimensional drawings of the CentriVap.
No person or any hazardous material should be within 12 inches of the CentriVap while it is operating.
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5
Original instructions
CChhaapptteerr 33:: GGeettttiinngg SSttaarrtteedd
Now that the site for your CentriVap Complete is properly prepared, you are ready to unpack, inspect, install, and test your CentriVap Complete. Read this chapter to learn how to:
Unpack and move your CentriVap Complete. Set up your CentriVap Complete. Connect the electrical supply source to your CentriVap Complete. Properly exhaust your CentriVap Complete. Safely use solvents with your CentriVap Complete.
CAUTION: The CentriVap Complete weighs 145 lbs. (66 Kg). The carton allows for lifting with a mechanical lift truck or hand truck. If you must lift the CentriVap manually, use at least two (2) persons and follow safe lifting guidelines.
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Chapter 3: Getting Started
6
Original instructions
Catalog # Description Volts Hz
1334500
1336100
1338000
1332600
1332700
7315020 CENTRIVAP COMPLETE (US) 115 60 X 7315021 CENTRIVAP COMPLETE ACID RESISTANT (US) 115 60 X 7315022 CENTRIVAP COMPLETE W/ HEAT BOOST (US) 115 60 X 7315023 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (US) 115 60 X 7315030 CENTRIVAP COMPLETE (EU) 230 50 X 7315031 CENTRIVAP COMPLETE ACID RESISTANT (EU) 230 50 X 7315032 CENTRIVAP COMPLETE W/ HEAT BOOST (EU) 230 50 X 7315033 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (EU) 230 50 X 7315040 CENTRIVAP COMPLETE (US) 230 60 X 7315041 CENTRIVAP COMPLETE ACID RESISTANT (US) 230 60 X 7315042 CENTRIVAP COMPLETE W/ HEAT BOOST (US) 230 60 X 7315043 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (US) 230 60 X 7315060 CENTRIVAP COMPLETE UK 230 50 X 7315061 CENTRIVAP COMPLETE ACID RESISTANT UK 230 50 X 7315062 CENTRIVAP COMPLETE W/ HEAT BOOST UK 230 50 X 7315063 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (UK) 230 50 X 7315070 CENTRIVAP COMPLETE (CHINA) 230 50 X 7315071 CENTRIVAP COMPLETE ACID RESISTANT (CHINA) 230 50 X 7315072 CENTRIVAP COMPLETE W/ HEAT BOOST (CHINA) 230 50 X 7315073 CENTRIVAP COMPLETE ACID RESISTANT W/ HEAT BOOST (CHINA) 230 50 X
Power Cords
Power Cord Part #
Plug Type
1334500
NEMA 5-15P
1336100
CEE 7/7
1338000
NEMA 6-15P
1332600
BS 1363
1332700
CH1-10P
Unpacking Your CentriVap
Carefully unpack your CentriVap and inspect it for damage that may have occurred in transit. If your CentriVap is damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.
NOTE: Do not return goods without the prior authorization of Labconco. Unauthorized returns will not be accepted. If your CentriVap was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damage.
Do not discard the carton or packing material for your CentriVap until you have checked all of the components and installed and tested the CentriVap.
CentriVap Components
As previously mentioned, the CentriVap Complete is available in 115V or 230V. Locate the model of CentriVap you received in the following table. Verify that the components listed are present and undamaged.
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Chapter 3: Getting Started
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Original instructions
Part #
Component Description
7314000
User’s Manual
7396206
Coupling Insert
7455100
Rotor, 12-13 mm (Standard Models)
7455101
Rotor, 12-13 mm PTFE (Acid Resistant Models)
7450200
Glass Jar
Plus the following:
If you do not receive one or more of the components listed for your CentriVap, or if any of the components are damaged, contact Labconco Corporation immediately for further instructions.
Setting Up Your CentriVap
After you verify receipt of the proper components, move your CentriVap to the level and sturdy location where you want to install it. There is no need to secure the CentriVap to the work surface. Then, follow the steps listed below.
Emergency Access Into the Chamber
The CentriVap is designed to prevent access to the chamber in the event of a power disruption. If it is necessary to open the lid when there is no electrical power connected to the CentriVap, insert a small screwdriver or similar instrument into the small round hole on the left side of the case near the top behind the control panel. This will unlock the lid latch mechanism. While holding the screwdriver in place, raise the lid with the other hand.
CAUTION: Never attempt to defeat the latch or open the lid while the CentriVap is running. Personnel injury can result from moving parts and chemicals.
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Chapter 3: Getting Started
8
Original instructions
Exhaust Port
If the CentriVap is not located in a fume hood, attach one end of a user-supplied 3/8" ID hose to the exhaust port on the back of the unit. Clamp securely. Route the other end to a fume hood or other laboratory ventilation device.
Electrical Connection
Plug the power cord into the receptacle on the back of the CentriVap Concentrator and plug the other end into a suitable power receptacle.
Rotor Installation
Place the rotor onto the shaft of the Concentrator. Rotate the rotor slightly to engage the drive pin in the shaft with the slots in the rotor hub. The top of the shaft should be in line with the top of the rotor hub.
IMPORTANT: Do not use a rotor if it shows any signs of damage.
Chemical Resistance of CentriVap Components
Your CentriVap Complete is designed to be chemical resistant to most compounds that are commonly used in the concentration processes that are performed in it. However, by necessity, the CentriVap is comprised of a number of different materials, some of which may be attacked and degraded by certain chemicals. The degree of degradation is obviously dependent on the concentration and duration of exposure. Some major components of the CentriVap that are susceptible to degradation are as follows:
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Chapter 3: Getting Started
9
Original instructions
Acids
Acids
Bases Solvents
Bases Solvents
COMPONENT MATERIAL
Acetic Acid 20%
Boric Acid
Formic Acid
Hydrobromic Acid 20%
Hydrochloric Acid 20%
Nitric Acid 20%
Sulfuric Acid 10%
Trifluoroacetic Acid (TFA)
Ammonium Hydroxide
Acetone
Acetonitrile
Chloroform
Dimethyl Formamide
Dimethyl Sulfoxide (DMSO)
Ethanol
Ethyl Acetate
Hexanes
Isoproponal
Methanol
Methylene Chloride
Methyl t-Butyl Ether (MTBE)
Toluene
Water
Standard CentriVap
Lid Acrylic C D C D D D D C D D C D D
Bearings High Carbon Steel D D D D D D D D D D D
Lid Gasket EPDM D D D D D C C D
Rotor Shaft Stainless Steel D D D
Valve Stainless Steel D D D
O Rings Viton (Fluorocarbon) C C C D C
Fittings Polypropylene D C
Rotor Anodized Aluminum D D
Rotor Hub Acetal (Delrin) C C C D D D C D C
Tubing PVC D C D D D D D D D C D D D D
Acid Resistant CentriVap
Chamber Teflon coated Aluminum
Lid Glass
Bearings Stainless Steel D D D
Lid Gasket EPDM D D D D D C C D
Rotor Shaft Hastelloy
Valve Teflon D
O Rings Viton (Fluorocarbon) C C C D C
Fittings Polypropylene D C
Rotor Teflon coated Aluminum
Rotor Hub Polypropylene D C
Tubing PVC D C D D D D D D D C D D D D
Cold Trap -50°C
Chamber Stainless Steel D D D
Lid Acrylic C D C D D D D C D D C D D
Cold Trap Gasket Neoprene D D C D D C D D D D D C D
Cold Trap -84°C & -105°C
Chamber Stainless Steel D D D
Lid Stainless Steel D D D
Cold Trap Gasket Neoprene D D C D D C D D D D D C D
C- Moderate Degradation- Questionable use
D- Severe Degradation- Infrequent use recommended- immediate thorough cleaning required
Epoxy coated Aluminum
Chamber
CDC
D
When using compounds in the CentriVap that are hostile to the materials of construction, it is imperative that the equipment is appropriately maintained.
After each run, clean up all residues, spills and materials that might have
splashed in the chamber using agents suitable for the substance involved.
Drain the cold trap immediately after the collected ice is melted to prevent
corrosive liquids from residing in the trap. Flush out the trap with water after draining.
DO NOT chip ice off the cold trap walls as damage may occur.
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Chapter 3: Getting Started
10
Original instructions
Solvent Safety Precautions
CAUTION: The CentriVap is not classified as “explosion proof.” It has been designed with safety as a primary consideration and should be used in a
prudent manner using “good laboratory practices.” It has been designed for
use with compounds as described in the United States National Electrical Code Class I, Group D. The heater may be programmed to run as hot as 100°C, however, the heater element may normally run at 110°C. A thermal fuse limits the heater to a maximum temperature of 120°C. It is important that the solvents used are compatible with these temperatures. Do not evaporate solvents that have an autoignition temperature below 180°C. Do not evaporate solvents that are classified as Group A, B, or C by the National Electrical Code. Evaporate only non-flammable or Group D solvents with autoignition temperatures 180°C or above. Use of other compounds could cause an explosion.
CAUTION: Solvents used in the CentriVap may be flammable or hazardous. Use extreme caution and keep sources of ignition away from the solvents. When using flammable or hazardous solvents, the CentriVap should be operated inside a fume hood.
If a sample is spilled in the chamber it must immediately be cleaned up. Hazardous materials such as strong acids or bases, radioactive substances and volatile organics, must be handled carefully and promptly cleaned up if spilled.
Do not store flammable or hazardous solvents within 12 inches (300 mm) of the CentriVap.
IMPORTANT: Various Federal, State or local regulations may govern the disposal of substances used in connection with this equipment. All users of this equipment are urged to become familiar with any regulations that apply
in the user’s area concerning the dumping of waste materials in or upon
water, land or air and to comply with such regulations.
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11
Original instructions
CChhaapptteerr 44:: UUssiinngg YYoouurr CCeennttrriiVVaapp CCoom
mpplleettee
After your CentriVap Complete has been installed as detailed in Chapter 3: Getting Started, you are ready to begin using your CentriVap. Read this chapter
to learn how to:
Set operating parameters. Operate the controls. Properly select and position glassware inside your CentriVap. Understand the display. Interrupt a cycle after it has begun.
NOTE: See Appendix C: CentriVap Specifications for electrical requirements.
NOTE: Do not store or stack supplies or equipment on top of the CentriVap.
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Chapter 4: Using Your CentriVap Complete
12
Original instructions
Planning
Thoroughly understand procedures and the equipment operation prior to beginning work. The unique performance of the CentriVap is dependent upon the proper balance of heat, vacuum and centrifugal force. If the proper balance is not established, it is possible to damage or lose a portion of the sample. Therefore, if you are unfamiliar with the CentriVap or are attempting a new protocol, it may be helpful to make a trial run that is void of the sample you are attempting to concentrate.
Glassware Selection
Sample tubes should be filled no more than half full with substances having a density no greater than 1400 kg/m3. Select the size of the sample tube so it is compatible with the rotor and the desired sample size. Tubes should not be loose in the rotor. Rotors are available with holes for various size tubes. Refer to Chapter 6: Accessories for your CentriVap Complete for available rotor sizes.
Loading Glassware Into the CentriVap
Smooth operation of the CentriVap is dependent upon proper balance of the machine. Therefore, if less than a full load of samples is run, it is important to load samples into the CentriVap in a fairly symmetrical manner distributing the weight of the samples evenly in the sample rotor.
Temperature Setting Guidelines
The evaporation rate achieved by the CentriVap is dependent upon a variety of factors. These include the nature of the solvent, the temperature and the pressure in the vacuum system.
As a general guideline, to speed the evaporation process, the CentriVap temperature should be set as high as possible as long as the temperature will not damage the sample or cause the sample to bump.
Time Setting Guidelines
The CentriVap Complete has two timers. The “RUN TIME” turns the entire concentrator off after the user set period of time. This stops the rotation, turns off the heater and vacuum pump and bleeds vacuum from the system. The heater may be turned off prior to the concentrator turning off so heat-sensitive samples may be protected from exposure to excessive heat after the solvent has evaporated. To turn the heater off prior to stopping the entire system, enter a set
time into the memory at the “HEATER TIME.” When the programmed time
expires, the heater will turn off but the rotation and vacuum will continue until the run time expires.
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Chapter 4: Using Your CentriVap Complete
13
Original instructions
The tables in Appendix C: CentriVap Specifications indicate approximate times required to evaporate various common solvents. Actual times must be determined by the user. The CentriVap can be set to alarm after a preset period of operation. When the time expires, the CentriVap will give an audible alarm and turn itself off.
Heat Boost Operation
Some CentriVap models are equipped with a secondary “Heat Boost” heater,
which is positioned on the sidewall of the chamber near the top. It provides additional heat to the samples to speed evaporation. The “Heat Boost” heater is controlled by the microprocessor to maintain the set point temperature at the bottom of the chamber to prevent excessive overheating of the samples.
CentriVap Controls
The control panel for the CentriVap is shown below with a description about its function.
1. Display – The liquid crystal display (LCD) shows set point parameters and
actual measured conditions.
2. Program Buttons – Used to initiate the start of a run with the use of just one
button.
3. Run/Stop Button – Used to start or stop a run.
4. Preheat Button – Used to turn on the heater to preheat the chamber prior to
loading samples.
5. Increase Button – When pressed, the last selected set point will increase.
6. Decrease Button – When pressed, the last selected set point will decrease.
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Chapter 4: Using Your CentriVap Complete
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Original instructions
7. Set Point Select Button – To select a parameter to change, press the select
button. Arrows on the display will point to the parameter that may be altered.
8. Heat Boost Button – If the model is so equipped, pressing the button will
activate or deactivate the auxiliary Heat Boost heater.
9. Cold Trap Button – Used to start or stop the refrigeration system for the cold trap.
Operating the CentriVap Controls
Preheat:
To preheat the chamber, press the “PREHEAT” button. The display will show:
SP: XXX ACT: XXX
Press the increase or decrease button until the desired set point (SP) is displayed. The actual chamber temperature, (ACT) is displayed to the right. When power is being supplied to the heaters, a bar under the actual temperature will illuminate.
The chamber will continue to preheat until either the “PREHEAT” button is
pressed again or the “RUN” button is pressed.
Select existing program:
Operating parameters can be stored in memory so protocols can be repeated. Nine programs can be stored. To select a program, press the set point “SELECT” button until arrows point to the program number indicating that this set point can be run or altered. To change the program number, press the increase or decrease button until the desired program number is displayed. When the program number is changed, all its set points change also to indicate the last entered parameters for that program.
Store frequently run protocols in program 1, 2, or 3. Then, by pressing “PROG 1,” “PROG 2” or “PROG 3,” the stored program will be initiated without having
to press any other button. Pressing just the one button starts the rotor, the heater, the timers and the vacuum pump.
Change “Temperature” Set Point:
To change the “Temperature” set point, press the set point “SELECT” button until
arrows point to the “Temperature” set point, which can be changed from - (OFF)
up to 99° by pressing the increase or decrease buttons. The set point is 100°C if the display shows “HI.” The last entered set point is stored in memory.
Change “Run Time” or “Heater Time” set point:
To change the time set point, press the set point “SELECT” button until arrows point to the “Heater Time” or “Run Time” set point which can be changed from 1
to 999 minutes by pressing the increase or decrease buttons. If it is desired to have the CentriVap run continuously without alarming at the end of a time period,
press the increase button until the ”Run Time” display says “ON.” The last
entered set point is stored in memory.
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Chapter 4: Using Your CentriVap Complete
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Original instructions
If during a run the “STOP” button is pressed, the timers remember the time at
which stop occurred. If “RUN” is then pressed, the timers continue to count
down from the time at which they were stopped. If you are running program 1,
program 2 or program 3, pressing “PROG 1,” “PROG 2” or “PROG 3” resets the
timers to the original set point time and the CentriVap starts a new run. To reset the timers to the original set point time when running programs other
than program 1, program 2 or program 3, press the start button and hold it for five seconds. The display will indicate that the timer is reset.
Operating the CentriVap
CAUTION: To avoid personnel injury; Do not operate the CentriVap if the lid is scratched or nicked, or shows signs of damage. A damaged lid could fail under vacuum.
While the CentriVap is operating, do not lean on the lid, do not stand near it longer than necessary and do not place hazardous materials within 12 inches.
1. Turn “ON” the CentriVap power switch.
2. Press the Cold Trap “ON” switch. The “Y” in the display under the cold trap
will flash. When the trap reaches operating temperature the “Y” will
illuminate steady.
3. Select a program or set the set point parameters.
4. Preheat the chamber if desired.
5. Turn on Heat Boost if the CentriVap is so equipped and additional heat is
desired.
6. Place samples in vials. Normally the vials should be no more than half full.
Place vials in a rotor.
7. Load the rotor with samples into the chamber.
8. Close the lid.
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Chapter 4: Using Your CentriVap Complete
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Original instructions
9. Press “RUN.” If the display was showing set point parameters, it will change
to show actual parameters. Press “RUN” again. The “S” (STOP) in the display changes to “R” (RUN). The program on the display alternately displays “R” or the number of the program that is running. If you intend to
run program 1, 2 or 3, simply press “PROG 1,” “PROG 2” or “PROG 3” to quick start the CentriVap. The rotor will start, the vacuum break valve will close and the vacuum pump will start after the rotor reaches operating speed.
10. Set point parameters can be altered at any time during a run by first selecting
the parameter using the set point “SELECT” button and then pressing the
“INCREASE” or “DECREASE” switch.
11. If the run time set point is used, at the end of the set time an alarm sounds. All
functions cease.
12. Press “STOP” to terminate operation if the CentriVap has not already stopped
itself.
13. When the evaporation is complete, allow the rotor to stop moving, lift the lid
and remove the samples.
CentriZap Strobe Light
The CentriZap will enable you to see the samples as they are rotating in the rotor. To use the strobe light while the CentriVap is operating, remove the light from its holder on the front of the unit, press the trigger and shine the light on the samples in the rotor.
Operational Notes
As solvent is evaporated in the Concentrator and then condensed in the Cold Trap, it is normal for the Cold Trap temperature to rise as its load increases.
Depending on which solvent is used, the volume of the sample and the system operating parameters, the Cold Trap may warm up sufficiently to cause the
“READY” indicator to turn off. As the sample reaches completion, the load on the Cold Trap will decrease and its temperature will decrease. The “READY”
indicator will once again illuminate. When the CentriVap is turned on, the CentriVap returns to the same mode
(“RUN” or “STOP”) that it was in when the power was turned off. If the CentriVap was in the “RUN” mode when the power was turned off, when the
power is turned on, the CentriVap attempts to return to the programmed set points and continue the run.
To reset the timers to the original set point when running programs other than program 1, program 2 or program 3, press the start button and hold it for five seconds. The display will indicate that the timer is reset.
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Chapter 4: Using Your CentriVap Complete
17
Original instructions
Interrupting a Cycle After it Has Begun
At any time during a run, the cycle may be stopped by pressing the “STOP”
button. This shuts off all operating functions. After the rotor stops, the lid may be opened. If it is necessary to re-start the CentriVap, close the lid and press “RUN.” The CentriVap resumes operation at the same set point parameters and the timer continues to count down from the time at which the CentriVap was stopped.
Gas Ballast
The vacuum pump has a gas ballast that can be used to reduce condensation of vapors in the pump and exhaust line. This is most likely to occur if the Cold Trap is not running in conjunction with the evaporation process. The gas ballast is
OPEN if the arrow on the gas ballast cap is pointing “IN” towards the CentriVap.
The gas ballast is CLOSED if the cap is rotated 180°, so that the arrow is pointing “OUT” of the CentriVap.
Safety Precautions
Special precautions must be observed if the materials used in the CentriVap Concentrator are known to be hazardous, toxic, radioactive, or contaminated with pathogenic micro organisms. These actions should include but are not limited to the following:
Refer to the World Health Organization Laboratory Biosafety Manual,
paying special attention to information about centrifuges and the handling
of hazardous materials.
Operate or vent the CentriVap Concentrator inside a suitable fume hood or
ventilation device. Load rotors in a ventilation device.
Periodically inspect all parts of the CentriVap Concentrator including the
lid, gasket, chamber, plumbing components and rotors.
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18
Original instructions
CChhaapptteerr 55:: MMaaiinnttaaiinniinngg YYoouurr CCeennttrriiVVaapp CCoom
mpplleettee
Under normal operation, the CentriVap Complete requires little maintenance. The following maintenance schedule is recommended. Before servicing the CentriVap, disconnect electrical power. Special precautions must be observed if materials used in the CentriVap are known to be hazardous, toxic, radioactive or contaminated with pathogenic micro organisms. Before servicing, the CentriVap must be suitably decontaminated. Wear appropriate eyewear, gloves and other safety apparel.
As needed:
Before using any cleaning or decontamination method except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage equipment.
1. Clean up all spills; remove liquids from the chamber. Clean or decontaminate
2. Clean lid and gasket using soft cloth, sponge or chamois and a mild, non-
all surfaces using agents suitable for the substance spilled.
abrasive soap or detergent.
3. Utilization of acids requires immediate cleaning and neutralization after a run
or physical damage to the stainless steel collection chamber will result.
4. Check the Cold Trap for condensed or frozen solvents and dispose of
appropriately. Completely empty the trap before the next run.
5. If the media in the optional clear canister has changed color, discard and
replace the insert with a new insert. For the radiochemical trap insert, no indicator exists; therefore, it should be discarded after each use. In radioactive applications, the system should be monitored with a Geiger counter.
6. Check rotors for loose or missing parts. Tighten or replace as required. Do
not use defective rotors.
7. Inspect the chamber to insure that there are not cracks or structural damage.
Call Labconco if defects exist.
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Chapter 5: Maintaining Your CentriVap Complete
19
Original instructions
8. Check continuity of the protective earth between the ground terminal on the
power inlet and a bare metal housing panel. Contact Labconco if there is no continuity.
9. Repair any defects to the surface where the CentriVap is installed.
Monthly:
1. The rubber components on the CentriVap may eventually deteriorate and
require replacement. The effective life of rubber parts depends upon both their usage and the surrounding environment. Check all rubber hoses and gaskets and replace any that show signs of hardening, permanent set or deterioration.
2. Using a soft cloth, sponge or chamois and a mild, non-abrasive soap or
detergent, clean the glass lid.
3. Using a soft cloth, sponge, or chamois and a mild, non-abrasive soap or
detergent, clean the exterior surfaces of the unit. Liquid spray cleaners and polishes may be used on the exterior surfaces. Do not use solvents to remove stains from the exterior surfaces as they may damage the finish.
Annually:
1. Every 12 months, or more often, if the Cold Trap is operated in a dusty
environment, the refrigeration system condenser of the Cold Trap should be cleaned. Using a vacuum cleaner with brush attachment, clean the condenser to ensure proper airflow for peak performance. Disconnect power before removing covers.
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20
Original instructions
CChhaapptteerr 66:: AAcccceessssoorriieess ffoorr YYoouurr CCeennttrriiVVaapp CCoom
mpplleettee
The configuration of your CentriVap Complete can be changed to accommodate your needs. Read this chapter to learn how to:
Install a vacuum gauge.
Install a secondary chemical trap.
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Chapter 6: Accessories for Your CentriVap Complete
21
Original instructions
PART #
DESCRIPTION
7462900
7462901*
Rotor (DNA) Holds (72) 0.5 ml microcentrifuge tubes and (60) 1.5 ml microcentrifuge tubes or (60) 2.0 ml microcentrifuge tubes
7450700
7450701*
Rotor (1.5 ml) Holds (132) 1.5 ml microcentrifuge tubes or (132) 2.0 ml microcentrifuge tubes
7455000
7455001*
Rotor (15 ml) Holds (44) 12 x 55 mm tubes or (36) 12 x 75 mm tubes or (36) 12 x 95 mm tubes or (36) 13 x 75 mm tubes or (36) 13 x 100 mm tubes and (24) 16 x 100 mm tubes or (18) 16 x 120 mm conical tubes or (18) 17 x 95 mm tubes or (18) 17 x 100 mm centrifuge tubes or (18) 17 x 120 mm tubes
7455100
7455101*
Rotor (12-13 mm)
Holds (40) 1.5 ml microcentrifuge tubes or (40) 2.0 ml microcentrifuge tubes and (16) 12 x 55 mm tubes or (100) 12 x 75 mm tubes or (64) 12 x 95 mm tubes or (100) 13 x 75 mm tubes or (64) 13 x 100 mm tubes
7455200
7455201*
Rotor (50 ml) Holds (32) 1.5 ml microcentrifuge tubes or (32) 2.0 ml microcentrifuge tubes and (12) 28 x 115 mm conical tubes or (12) 28 x 135 mm conical tubes or (12) 28 x 140 mm conical tubes
7553100
7553101*
Rotor (100 ml pear shaped flask) Holds (8) 100 ml flasks
7553000
7553001*
Rotor (6 microtiter) Holds (6) Standard 96 well plates or (3) Deep well plates
The following accessories are available for the CentriVap Complete.
*PTFE Coated
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Chapter 6: Accessories for Your CentriVap Complete
22
Original instructions
PART #
DESCRIPTION
7461900
7461901*
Rotor (96 well plate) Holds (4) Standard 96 well plates or (2) Deep well 96 well plates
7460900
Clear Canister – Accommodates inserts listed below
7814800
Acid Trap Insert
7814900
Moisture Trap Insert
7995600
Ammonia Trap Insert
7815000
Radiochemical Trap Insert
7815200
Solvent Trap Insert
USER SUPPLIED VACUUM TUBING
Installing a Vacuum Gauge
A user-supplied vacuum gauge may be attached to the CentriVap to monitor the vacuum level. Attach the vacuum gauge to the barb end of the Quick Disconnect Coupling fitting that was supplied with the CentriVap using a length of suitable rubber hose. The Quick Disconnect Coupling fitting can then be pushed into the mating connector on the rear left side of the CentriVap. The Quick Disconnect Coupling fitting can be removed from the mating connector by pressing on the tab on the connector and then pulling out the Quick Disconnect Coupling fitting. Always remove the Quick Disconnect Coupling from the CentriVap when a vacuum gauge is not attached to it.
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Chapter 6: Accessories for Your CentriVap Complete
23
Original instructions
Installing a Secondary Chemical Trap
An accessory secondary chemical trap is available to minimize the exhausting of solvents into the atmosphere. Attach the bracket to the right hand side of the housing using the screws provided. Attach the hose from the outlet on the vacuum pump to the “out” connector of the canister housing. Unscrew the clear blow of the canister housing from the head. Remove both the upper and lower caps from the filter cartridge and insert the small end of the cartridge into the hole in the center of the head. Reinstall the clear bowl.
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24
Original instructions
CChhaapptteerr 77:: TTrroouubblleesshhoooottiinngg
DISPLAY ERROR CODE
CAUSE
CORRECTIVE ACTION
Heat Sensor
Sensor failure
Replace sensor assembly.
Connection failure
Repair connection.
Close Lid
Lid open
Close lid.
Latch Fail
Solenoid failure
Check component.
Switch or sensor failure
Check connections.
Mem Fail P1
Memory failure
Push program button #1.
New memory IC chip
Push program button #1.
Bad memory IC chip
Call Labconco – Replace IC chip or control PCB.
MOTOR ERROR
Defective motor
Replace motor.
Hall effect sensor failure
Replace sensor. Wire failure
Replace wire.
Refer to the following if your CentriVap fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance. The following failure codes may appear on the display when problems are sensed by the internal self check routine.
CAUTION: Disconnect power before corrective action is taken.
Product Service 1-800-522-7658
Chapter 7: Troubleshooting
25
Original instructions
PROBLEM
CAUSE
CORRECTIVE ACTION
Unit will not operate
Unit not connected to electrical power
Connect unit to proper electrical receptacle.
Circuit breaker blown
Correct electrical problem and reset circuit breaker by pressing button.
Lid open
Close lid.
Excessive vibration
Sample tubes not located symmetrically in rotor
Reposition sample tubes.
Sample odor in lab
Vent hose exhausting into lab area
Redirect hose to fume hood.
Evaporation rate is reduced
Heater inoperable
Contact Labconco. Vacuum pump failure
Check pump.
Obstruction in hose
Remove obstruction or replace hose.
Lack of adequate vacuum
See below.
No vacuum/poor vacuum
Pump not on
Turn on pump.
Control valve open
Check control valve.
Leaks in lines or connectors or gasket
Locate and repair.
Foreign material on lid gasket
Clean gasket and lid.
Pump is not functioning properly
Check pump by locating vacuum gauge closer to pump and close off rest of system.
If pump is faulty, seek authorized service or replace pump.
Cold Trap or Concentrator gasket is not sealing properly
Check gasket for cleanliness. Adjust gasket as needed. Cold Trap lid not seated
Hold lid down until vacuum is initiated.
Ice formed on Cold Trap lid sealing surface
Defrost and wipe dry.
Other corrective actions for potential problems are as follows:
Product Service 1-800-522-7658
Chapter 7: Troubleshooting
26
Original instructions
PROBLEM
CAUSE
CORRECTIVE ACTION
Recovery of condensate in Cold Trap is less than normally expected
Cold Trap is not ON
Check to make sure switch is ON and condensing unit fan is moving air out of the sides of the cabinet.
Cold Trap does not cool down
Turn vacuum pump OFF and allow Cold Trap to cool for at least 30 min. to reach temperature.
-50°C temperature can be checked with a solvent thermometer or digital thermometer.
Unit starts and shuts off
Latch optical sensor improperly calibrated
Unplug power cord. Wait 10 seconds. Plug in power cord.
Product Service 1-800-522-7658
27
Original instructions
AAppppeennddiixx AA:: CCeennttrriiVVaapp CCoom
mppoonneennttss
The following pages list components that are available for your CentriVap Complete. The parts shown are the most common replacement parts. If other parts are required, contact Product Service.
Product Service 1-800-522-7658
Appendix A: CentriVap Components
28
Original instructions
Product Service 1-800-522-7658
29
Original instructions
AAppppeennddiixx BB:: CCeennttrriiVVaapp DDiim
meennssiioonnss
Product Service 1-800-522-7658
30
Original instructions
AAppppeennddiixx CC CCeennttrriiVVaapp SSppeecciiffiiccaattiioonnss
This Appendix contains technical information about the CentriVap including specifications, environmental operating conditions and evaporation rates.
Mechanical Specifications
Weight: 145 lbs. (66 kg) Dimensions: See Appendix B
Electrical Specifications
Nominal amperage for 115V/60 Hz CentriVap: 12A. Nominal amperage for 230V/50Hz CentriVap: 6.5A. Nominal amperage for 230V/60Hz CentriVap: 6.5A. Phase: Single Heater Power: 300 watts – Main heater Heater Power: 300 watts – Heat boost Rotor Speed: Up to 1,725 RPM
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Appendix C: CentriVap Specifications
31
Original instructions
Environmental Conditions
Indoor use only. Maximum altitude: 6562 feet (2000 meters). Ambient temperature range: 41° to 104°F (5° to 40°C). Maximum relative humidity: 80% for temperatures up to 88°F (31°C),
decreasing linearly to 50% relative humidity at 104°F (40°C).
Main supply voltage fluctuations not to exceed ±10% of the nominal
voltage.
Transient overvoltages according to Installation Categories II
(Overvoltage Categories per IEC 1010). Temporary voltage spikes on the
AC input line that may be as high as 1500V for 115V models and 2500V
for 230V models are allowed.
Used in an environment of Pollution degrees 2 (i.e., where normally only
non-conductive atmospheres are present). Occasionally, however, a
temporary conductivity caused by condensation must be expected, in
accordance with IEC 664.
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Appendix C: CentriVap Specifications
32
Original instructions
Methylene Chloride
bp 40
Tube Size
(ml)
Number
of
Samples
Sample
Size (ml)
Heater
Temp
(C)
Heat
Boost
Vacuum
Pump
Time to
Dry
(min)
Cold Trap
Rate
Overall
(ml/min)
50
12
25
45
off
Diaphragm
93
yes
3.23
50
12
25
45
on
Diaphragm
89
yes
3.37
15
18
10
45
off
Diaphragm
57
yes
3.16
15
18
10
45
on
Diaphragm
55
yes
3.27
Toluene
bp 111
50
12
25
45
off
Diaphragm
213
yes
1.41
50
12
25
45
on
Diaphragm
151
yes
1.99
50
12
25
100
off
Diaphragm
63
yes
4.76
50
12
25
100
on
Diaphragm
57
yes
5.26
15
18
10
45
off
Diaphragm
93
yes
1.94
15
18
10
45
on
Diaphragm
85
yes
2.12
15
18
10
100
off
Diaphragm
42
yes
4.29
15
18
10
100
on
Diaphragm
37
yes
4.86
Acetonitrile
bp 82
50
12
25
45
off
Diaphragm
182
yes
1.65
50
12
25
45
on
Diaphragm
178
yes
1.69
50
12
25
100
off
Diaphragm
97
yes
3.09
50
12
25
100
on
Diaphragm
89
yes
3.37
15
18
10
45
off
Diaphragm
117
yes
1.54
15
18
10
100
off
Diaphragm
63
yes
2.86
15
18
10
100
on
Diaphragm
53
yes
3.40
1.5
132 1 45
off
Diaphragm
57
yes
2.32
1.5
132 1 60
off
Diaphragm
44
yes
3.00
1.5
132 1 75
off
Diaphragm
37
yes
3.57
1.5
132
1
100
off
Diaphragm
27
yes
4.89
1.5
132
1
100
on
Diaphragm
25
yes
5.28
Methanol
bp 65 50
12
25
45
off
Diaphragm
245
yes
1.22
15
18
10
45
off
Diaphragm
145
yes
1.24
1.5
132 1 45
off
Diaphragm
75
yes
1.76
1.5
132 1 60
off
Diaphragm
58
yes
2.28
1.5
132 1 75
off
Diaphragm
51
yes
2.59
1.5
132
1
100
off
Diaphragm
38
yes
3.47
1.5
132
1
100
on
Diaphragm
34
yes
3.88
Evaporation Rates
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Appendix C: CentriVap Specifications
33
Original instructions
Water
bp 100
Tube Size
(ml)
Number
of
Samples
Sample
Size (ml)
Heater
Temp
(C)
Heat
Boost
Vacuum
Pump
Time to
Dry
(min)
Cold Trap
Rate
Overall
(ml/min)
50
12
25
45
off
Diaphragm
1120
yes
0.27 50
12
25
100
off
Diaphragm
416
yes
0.72 50
12
25
100
on
Diaphragm
383
yes
0.78 15
18
10
45
off
Diaphragm
618
yes
0.29 1.5
132 1 45
off
Diaphragm
358
yes
0.37
1.5
132 1 60
off
Diaphragm
251
yes
0.53
1.5
132 1 75
off
Diaphragm
193
yes
0.68 1.5
132
1
100
off
Diaphragm
137
yes
0.96 1.5
132
1
100
on
Diaphragm
122
yes
1.08
Vacuum Pump – Rated at 7 mbar ultimate vacuum and 38 L/min displacement
Chamber preheated prior to each run to run temp.
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