Labconco 3970202, 3970205, 3970222, 3970225, 3970305 User Manual
...
Purifier®Filtered PCR Enclosures
User’s Manual
Labconco’s Mascot,
Labby the LABster
Models
3970202, 3970205, 3970222, 3970225,
3970302, 3970305, 3970322, 3970325,
3970402, 3970405, 3970422, 3970425
Labconco Filtered PCR Enclosure Manual
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.
The warranty for all Labconco products will expire one year from date of installation or two years from
date of shipment from Labconco, whichever is sooner, except the following:
• Purifier® Delta® Series Biological Safety Cabinets and PuriCare® Animal Laboratory Research
Enclosures carry a three-year warranty from date of installation or four years from date of
shipment from Labconco, whichever is sooner.
• Carts carry a lifetime warranty.
• SteamScrubber and FlaskScrubber Glassware Washers carry a two-year warranty from date of
installation or three years from date of shipment from Labconco, whichever is sooner.
• Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the event of a
warranty claim, contact Labconco Corporation or the dealer who sold you the product. If the cause is
determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or replace all
defective parts to restore the unit to operation. Under no circumstances shall Labconco Corporation be
liable for indirect, consequential, or special damages of any kind. This statement may be altered by a
specific published amendment. No individual has authorization to alter the provisions of this warranty
policy or its amendments. Lamps and filters are not covered by this warranty. Damage due to corrosion or
accidental breakage is also not covered.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.
.
Part #3938200, Rev. -
ECO B731
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CHAPTER 1: INTRODUCTION 1
ISO Class 5 Definition 2
Polymerase Chain Reaction Definition 2
About This Manual 3
Typographical Conventions 4
CHAPTER 2: PREREQUISITES 5
Support, Vibration & Movement Requirements 6
Location and Air Current Requirements 6
Airflow Specifications 6
Electrical Requirements 7
Space Requirements 7
CHAPTER 3: GETTING STARTED 8
Unpacking Your Enclosure 9
Installing the Filtered PCR Enclosure on a Supporting
Structure and Work Surface 9
Verifying HEPA Filter Integrity 12
Connecting the Electrical Supply Source to the Filtered PCR
Enclosures 12
Setting the Downflow Velocity with Speed Control 13
Validating the Filtered PCR Enclosure 13
Sealing the Filtered PCR Enclosure to the Work Surface 14
CHAPTER 4: PERFORMANCE FEATURES AND
Components 16
Safety Precautions 20
CHAPTER 5: APPROPRIATE APPLICATIONS FOR YOUR
Routine Daily Work Procedures 23
Suitable Applications 24
HEPA Filter Applications, Suitability & Guidelines 25
CHAPTER 6 MAINTAINING YOUR FILTERED PCR
Routine Maintenance Schedule 27
When to Replace HEPA Filters 27
How to Install a New HEPA Filter 28
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SAFETY PRECAUTIONS 15
FILTERED PCRT ENCLOSURE 23
ENCLOSURE 26
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HEPA Filter Leak Test 30
Setting the Downflow Velocity with the Speed Control Adjustment 32
Calibrate and Operate the Airflow Monitor 32
Initial Certification 36
Re-Certification 36
Fluorescent Light Replacement 36
UV Light Replacement 37
Motorized Impeller Replacement 37
Speed Control Replacement 39
CHAPTER 7 ACCESSORIZING & MODIFYING YOUR
FILTERED PCR ENCLOSURE 40
CHAPTER 8 TROUBLESHOOTING & SERVICER
OPERATING LOG 43
APPENDIX A: FILTERED PCR ENCLOSURE COMPONENTS
AND REPLACEMENT PARTS 47
APPENDIX B: DIMENSIONS 52
APPENDIX C: FILTERED PCR ENCLOSURE
SPECIFICATIONS 54
APPENDIX D: REFERENCES 59
DECLARATION OF CONFORMITY 60
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Congratulations on your purchase of a Labconco Purifier® Filtered
PCR Enclosure. Your enclosure provides a particulate free
environment for Polymerase Chain Reaction experiments. It is the
result of Labconco’s more than 30 years experience in
manufacturing filtered enclosures.
These enclosures will effectively provide product protection from
airborne particulate matter. During operation, room air is drawn
through the prefilter on top, forced through the blower and then
through the HEPA filter and diffuser. The clean air in the work
area in the enclosure meets or exceeds ISO Standard Class 5
conditions defined per ISO 14644-1 and Class 100 conditions as
defined by Federal Standard 209E. The enclosure is set to deliver
nominal downward airflow of 45-65 feet per minute. Each
enclosure uses a single HEPA filter, which is rated at least 99.99%
efficient for 0.3-micron particles. The Purifier Filtered PCR
enclosures offer many unique features to enhance performance. A
variable solid-state digital timer provides a UV light to destroy
contaminating DNA and RNA in the work area. The UV light
automatically turns off to prepare for the next experiment. To take
full advantage of all features, please acquaint yourself with this
manual and keep it handy for future reference. If you are
unfamiliar with how Filtered PCR Enclosures operate, please
review Chapter 4: Performance Features and Safety Precautions
before you begin working. Even if you are an experienced user,
please review Chapter 5: Using Your Filtered PCR Enclosure,
which describes the enclosure features.
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Chapter 1: Introduction
Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones.
classification
number (N)
ISO Class 1 10 2
ISO Class 2 100 24 10 4
ISO Class 5 Definition
Airborne particulate cleanliness inside any PCR Enclosure is
designated by ISO Class 5, which is equivalent to 3520 particles
0.5 µm or larger per cubic meter of air per ISO Standard 14644-1.
ISO Class 5 cleanliness is illustrated in the table to follow and is
equivalent to Class 100 air conditions as defined by Federal
Standard 209E. Class 100 is equal to 100 particles 0.5 µm or
larger per cubic foot of air.
ISO
Maximum concentration limits (particles/m3 of air) for particles equal to and
larger than the considered sizes shown below (concentration limits are
calculated in accordance with 3.2 of Standard 14644-1)
0.1 µm 0.2 µm 0.3 µm 0.5 µm 1 µm 5 µm
ISO Class 3 1 000 237 102 35 8
ISO Class 4 10 000 2 370 1 020 352 83
ISO Class 5 100 000 23 700 10 200 3 520 832 29
ISO Class 6 1 000 000 237 000 102 000 35 200 8 320 293
ISO Class 7 352 000 83 200 2 930
ISO Class 8 3 520 000 832 000 29 300
ISO Class 9 35 200 000 8 320 000 293 000
Table 1-1 ISO Classification Number (N)
Polymerase Chain Reaction
Definition (PCR)
Polymerase Chain Reaction (PCR), is a laboratory process in
which a particular DNA segment from a mixture of DNA chains is
rapidly replicated, producing a large, readily analyzed sample of a
piece of DNA. In PCR, DNA is immersed in a solution containing
the enzyme DNA polymerase, unattached nucleotide bases (the
subunits that DNA is composed of), and “primers”, short
sequences of nucleotides designed to bind with an end of the
desired DNA segment. Two primers are used: one primer binds at
one end of the desired segment on one of the two paired DNA
strands and the other primer binds at the other end but on the other
strand. The solution is heated to break the bonds between the
strands of the DNA. When the solution cools, the primers bind to
the separated strands, and DNA polymerase quickly builds a new
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Chapter 1: Introduction
strand by joining the free nucleotide bases to the primers. When
this process is repeated, a strand that was formed with one primer
binds to the other primer, resulting in a new strand that is restricted
solely to the desired segment. Thus, the region of DNA between
the primers is selectively replicated. Further repetitions of the
process can produce billions of copies of a small piece of DNA in
several hours. PCR was developed in 1985 by Kary B. Mullis,
who was awarded the 1993 Nobel Prize in chemistry for his work.
It is used in a broad range of applications from DNA fingerprinting
to medical tests to identify diseases from the infectious agent’s
DNA. See also nucleic acid. The Concise Columbia Encyclopedia
is licensed from Columbia University Press. Copyright © 1995 by
Columbia University Press. All rights reserved.
About This Manual
This manual is designed to help you learn how to install, use, and
maintain your Filtered (PCR) Enclosure.
Chapter 1: Introduction provides a brief overview of the Filtered
PCR Enclosure, explains the organization of the manual, and
defines the typographical conventions used in the manual.
Chapter 2: Prerequisites explains what you need to do to prepare
your site before you install the Filtered PCR Enclosure. Electrical
and service requirements are discussed.
Chapter 3: Getting Started contains the information you need to
properly unpack, inspect, install, and certify the Filtered PCR
Enclosure.
Chapter 4: Performance Features and Safety Precautions explains
how the Purifier Filtered PCR Enclosure operates and the
appropriate precautions you should take when using it.
Chapter 5: Using Your Filtered Enclosure discusses the basic
operation of how to prepare, use and shut down your Filtered PCR
Enclosure.
Chapter 6: Maintaining Your Filtered PCR Enclosure explains
how to perform routine maintenance on the PCR Enclosure.
Chapter 7: Accessorizing Your Filtered PCR Enclosure explains
acceptable modifications to the PCR Enclosure or how to add
accessories.
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Chapter 1: Introduction
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2'
3' 4'
Chapter 8: Troubleshooting contains a table of problems you may
encounter while using the Filtered PCR Enclosure including
probable causes and suggested corrective actions.
Appendix A: Components and Replacement Parts contains labeled
diagrams of all of the components of the Filtered PCR Enclosure.
Appendix B: Dimensions contains comprehensive diagrams
showing all of the dimensions for the Filtered PCR Enclosure.
Appendix C: Filtered PCR Enclosure Specifications contains the
electrical requirements for Filtered PCR Enclosures. Wiring
diagrams are also included.
Appendix D: References lists the various resources available
addressing clean work stations and PCR Enclosures.
Typographical Conventions
Recognizing the following typographical conventions will help
you understand and use this manual:
• Book, chapter, and section titles are shown in italic type (e.g.,
Chapter 3: Getting Started).
• Steps required to perform a task are presented in a numbered
format.
• Comments located in the margins provide suggestions,
reminders, and references.
• Critical information is presented in boldface type in paragraphs
that are preceded by the exclamation icon. Failure to comply
with the information following an exclamation icon may result
in injury to the user or permanent damage to the enclosure.
• Critical information is presented in boldface type in paragraphs
that are preceded by the wrench icon. Only a trained certifier
or contractor should only perform these operations. Failure to
comply with the information following a wrench icon may
result in injury to the user or permanent damage to your PCR
Enclosure.
• Important information is presented in capitalized type in
paragraphs that are preceded by the pointer icon. It is
imperative that the information contained in these paragraphs
be thoroughly read and understood by the user.
• A number icon precedes information that is specific to a
particular model of enclosure. The 2' icon indicates the text is
specific to the 2-foot wide model. The 3' icon indicates the text
is specific to the 3-foot model, etc.
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Before you install the Filtered PCR Enclosure, you need to prepare
your site for installation. You must be certain that the area is level
and of solid construction. In addition, a dedicated source of
electrical power should be located near the installation site to
power the Filtered PCR Enclosure, and other apparatus.
Additionally, the enclosure should be strategically placed in the lab
to provide efficient workflow.
Carefully read this chapter to learn the requirements for your
installation site:
• The support, vibration and movement requirements.
• The location and air current requirements.
• The exhaust and blower requirements.
• The electrical power requirements.
• The space requirements.
Refer to Appendix B: Dimensions for complete enclosure
dimensions.
Refer to Appendix C: Filtered PCR Enclosure Specifications for
complete filtered enclosure electrical and environmental
conditions, specifications and requirements.
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Chapter 2: Prerequisites
Support, Vibration and Movement
Requirements
At a minimum, the supporting structure usually consists of a base
cabinet or base stand and chemically-resistant work surface.
Location and Air Current
Requirements
The Purifier Filtered PCR Enclosures have been designed to
provide particulate free air meeting ISO Class 5 conditions by
negating typical cross drafts and turbulence within the opening.
However, as a precautionary safety measure and a higher level of
quality management, it is recommended that the Filtered PCR
Enclosure be placed in an area away from:
• High traffic areas where walking might cause an air
• Overhead or wall HVAC diffusers, fans, radiators or other lab
• Doorways or windows that may be opened.
disturbance or be a nuisance.
equipment producing air currents.
Airflow Specifications
The Purifier Filtered PCR Enclosures use an integral motorized
impeller to draw room air from the top and through the enclosure.
This room air is then pushed down through the HEPA filter. The
enclosure is factory set to deliver 45-65 fpm downflow velocity
measured 6" below the upper diffuser. The Class 5 HEPA-filtered
air flows across the work area and exits out the front and back of
the enclosure. The Filtered PCR Enclosure provides a continuous
downflow of particulate free HEPA filtered air.
Data for the outflow exhaust volume, downflow velocities and outflow face velocities are listed for each Filtered PCR model below.
6
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Downflow
Enclosure
Width Model Description
2'
3'
4'
2' Filtered PCR Enclosure
3' Filtered PCR Enclosure
4' Filtered PCR Enclosure
Velocity
(fpm)
Electrical Requirements
30
40
50
60
30
40
50
60
30
40
50
60
OutFlow
Exhaust
Volume
(CFM)
120
165
210
250
180
250
310
370
240
330
420
500
Chapter 2: Prerequisite
Average
Outflow
Noise
Pressure
db(A)
53-57
58-60
60-61
62-63
58-60
60-62
61-63
63-54
60-62
62-64
66-69
68-70
Face
Velocity
(fpm)
85
115
145
170
85
115
145
170
85
115
145
170
Standard duplex electrical receptacles should be nearby for
connecting the Filtered PCR Enclosure, or other equipment. The
enclosure includes iris pass-throughs to allow electrical cords
through the back of the enclosure without leaving a large hole.
Space Requirements
The dimensions for all the different models are shown in Appendix
B: Dimensions.
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Now that the site for your Filtered PCR Enclosure is properly
prepared, you are ready to unpack, inspect, install, and validate
your enclosure. Read this chapter to learn how to:
• Unpack and move the enclosure.
• Set up the enclosure with the proper supporting structure
and work surface.
• Verify HEPA filters integrity.
• Connect the electrical supply.
• Set the downflow velocity with the speed control
adjustment.
• Validate enclosure airflow.
• Seal the enclosure to the work surface.
Depending upon which model you are installing, you may need
common mechanical and electrical installation tools in addition to
wrenches, ratchets, sockets, a nut driver set, a flat-blade
screwdriver, a Phillips screwdriver, and a carpenter level to
complete the instructions in the chapter.
Each enclosure model weighs between 125 to 195
lbs. each (55 to 85 kg). The shipping container
!
allows for lifting with a mechanical lift truck or
floor jack. If you must lift the enclosure
manually, follow safe-lifting guidelines. Do not
lift by the lower air foil.
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Unpacking the Enclosure
Chapter 3: Getting Starte
Carefully remove the shrink-wrap or carton on the enclosure and
inspect it for damage that may have occurred in transit. If
damaged, notify the delivery carrier immediately and retain the
entire shipment intact for inspection by the carrier.
DO NOT RETURN GOODS WITHOUT THE
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Do not discard the packing material until you have checked all of
the components and tested the enclosure.
We recommend that you do not remove the enclosure from its
shipping container until it is ready to be placed into its final
location. Move the unit by placing a flat, low dolly under the
shipping skid, or by using a floor jack.
!
PRIOR AUTHORIZATION OF LABCONCO.
UNAUTHORIZED RETURNS WILL NOT BE
ACCEPTED.
IF ENCLOSURE WAS DAMAGED IN TRANSIT,
YOU MUST FILE A CLAIM DIRECTLY WITH
THE FREIGHT CARRIER. LABCONCO
CORPORATION AND ITS DEALERS ARE NOT
RESPONSIBLE FOR SHIPPING DAMAGES.
Do not move the enclosure by tilting it onto a
hand truck.
The United States
nterstate Commerce
Commission rules
require that claims be
iled with the delivery
carrier within fifteen (15)
days of delivery.
Installing the Filtered PCR
Enclosure on a Supporting
Structure and Work Surface
Use caution when lifting or moving the enclosure.
When installing the enclosure onto a chemical-resistant work
surface or benchtop, ensure that the structure can safely support the
combined weight of the enclosure and any related equipment. The
work surface should be at least as wide and deep as the enclosure
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Chapter 3: Getting Started
to properly support it. The front of the enclosure should be aligned
within 0.30" (8mm) of the front of the work surface. Mounting
holes are provided in the Labconco accessory work surfaces to
secure the enclosure.
Work Surface Specifications
The work surface should be smooth, rigid and durable, such as a
chemical-resistant epoxy resin. The surface should be non-porous
and resistant to the materials used in conjunction with the Purifier
Filtered PCR Enclosure. The work surface should also contain a
dished recessed area for containing primary spills.
Filtered PCR Enclosure Work Surface Installation
1. Level the base cabinet or stand and the work surface. See
Figure 3-1.
2. Position the work surface in its intended location and with
the front of the work surface towards you. (Rear mounting
holes are located close to the rear edge.)
3. Secure the work surface to the base cabinet or stand with a
structural adhesive or silicone sealant.
4. Insert the supplied mounting screws in the four holes.
Allow a minimum of 1/8" clearance under the head of the
screw for positioning the enclosure.
5. Place the enclosure on the work surface and slide the rear
flange and front air foil flanges under the mounting screw
heads.
6. Tighten the four screws to complete the installation.
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Chapter 3: Getting Starte
Figure 3-1
Filtered PCR Enclosure Installation
Product Service 1-800-522-765
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Chapter 3: Getting Started
Verifying HEPA Filter Integrity
HEPA
HEPA
The HEPA filter is shipped installed with the gasket on the
downstream side. The HEPA filter is leak checked at Labconco.
A second leak check is recommended before using the enclosure
and at least annually thereafter. Consult your Safety Officer and
Chapter 6 for the HEPA Filter Leak Test. See Figure 4-2 for
HEPA filter location, HEPA filter gasket, and filter clamp bolts.
All seams downstream of the HEPA filter are jacketed by positive
pressure. This intrinsically safe design ensures Class 5 conditions
and a particulate free environment for PCR experiments.
HEPA Filter Appropriate Use
Filter Size
2'
3'
4'
Part No.
3707900
3707901
3707902
HEPA filters are high-efficiency particulate
air filters having a particulate removable
efficiency of 99.99% for particles with a
diameter of 0.3 micron.
Connecting the Electrical Supply
Source to the Filtered PCR
Enclosure
115V Models
Simply connect the 115V power cord supplied to the IEC electrical
supply plug on the back of the enclosure. If using at 50 Hz
operations, blower performance maximum airflow will be reduced
by 17%.
230V Models
The 230V is shipped without a plug. Install the appropriate plug
for your electrical specifications per local codes.
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Chapter 3: Getting Starte
Setting the Downflow Velocity
with the Speed Control
Adjustment of the speed control gives the correct downflow
velocity and is located behind the front panel. The downflow
velocity should be from 30-65 fpm for Class 100 (ISO Class 5)
conditions. ISO Class 5 clean air conditions are maximized at a
setting within this range. The Filtered PCR Enclosure is factory
set at 55 ± 10 fpm downflow velocity. (Consult your Safety
Officer for airflow recommendations for your application).
Working at the lowest downflow velocity appropriate for the
application will give the quietest operation. Downflow velocity
measurements are made using an anemometer. An electric
anemometer can be obtained from your laboratory supply dealer.
Downflow velocity measurements should be taken 6" below the
interior upper diffuser. Using a small Phillips screwdriver, adjust
the speed control to give the required downflow velocity. See
Figures 6-1 and 6-2 in Chapter 6 to locate the speed control. The
downflow velocity is increased by turning the speed control
counterclockwise and clockwise to decrease downflow velocity.
Validating the Filtered PCR
Enclosure
To determine the actual downflow velocity, airflow velocity
readings are taken. This should be done 6" down from the upper
diffuser located inside the top of the enclosure. The “average
downflow velocity” is achieved by taking readings in three rows
across the enclosure with the readings 6" from the ends and evenly
spaced every 6". Refer to the exhaust specifications table in
Chapter 2 for proper airflow volumes and downflow velocities for
your particular model. Validation should be completed by a
certified technician.
The Purifier Filtered PCR Enclosures have been tested at
Labconco before shipment and provide ISO Class 5 clean air
conditions inside the enclosure. Labconco also performed
extensive performance testing to validate the Filtered PCR’s for
product protection under ISO Class 5 conditions. For copies of
these validation reports, contact Labconco Customer Service.
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Chapter 3: Getting Started
Sealing the Filtered PCR
Enclosure to the Work Surface
When the Filtered PCR Enclosure has been set in place and wired,
it may be sealed at the work surface to prevent materials from
collecting under the walls. A bead of silicone sealant is
recommended to seal the Filtered PCR Enclosure to the work
surface.
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The Purifier Filtered PCR Enclosure is designed to provide
particulate free protection from airborne matter. During
operation, room air is drawn from the top and filtered by the
HEPA filter providing Class 5 conditions inside the enclosure.
The Purifier Filtered PCR Enclosure delivers Class 5 air
conditions when operating at downflow velocities of 30 to 65
feet per minute. The HEPA filter is jacketed by negative
pressure preventing contaminated air inside the enclosure and
ensuring Class 5 air conditions inside the enclosure. After each
Polymerase Chain Reaction (PCR) experiment, the variable
timed UV light can be run to decontaminate the work area from
DNA and RNA.
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The Purifier Filtered PCR Enclosure HEPA filter is located
behind the front panel. Users are encouraged to routinely
check airflow to satisfy Class 5 air conditions inside the
enclosure or purchase a model with an airflow monitor. For
additional surface decontamination inside the enclosure, users
are encouraged to use the variable digital timed UV light and
UV sash closure.
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