Labconco 1300 Series A2, eq_manual_thermo_1300_series_class_ii__a2 Sistemas para archivo y comunicación de imágenes (PACS)

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User Manual

1300 Series A2

Class II, Type A2 Biological Safety Cabinet

Operating Manual 7021355 Rev. 13

Thermo Scientific

MANUAL NUMBER 7021355

13 41335 12/12/16 Added note to Drain Valve installation on page 2-8 ccs

12 40525/HD-1941 9/1/16 Noted SmartPort as option ccs

11 31809 4/6/16 Updated operating hours of the device to filter life meter (pg 1-5) ccs

10 31745 2/25/15 Added GFCI outlet reset instructions ccs

Preface

1300 Series A2 i

This manual covers the models shown below:

Dear User,

Congratulations on your purchase of a Thermo Scientific 1300 Series Class II, Type A2 biological safety cabinet! Your 1300 Series A2 biological safety

cabinet has been tested and certified in accordance to NSF/ANSI 49, and is designed to protect the user, the environment, and your research from

harmful substances and cross-contamination. This user’s manual provides instructions on how to use the 1300 Series A2 most effectively and safely. A

printed copy of this user’s manual is available from Technical Services, if required.

Containment * Comfort * Convenience™

The 1300 Series A2 offers a unique range of product features that will enhance your safety and improve overall operating efficiency. Should you have

any questions on using this product or need further explanation of any of it’s features, please contact Technical Services (Page iv), or your local

distributor.

Stainless Steel Interior Coated Interior Size Voltage

10 inch opening 8 inch opening 10 inch opening 8 inch opening

1322 1341 1320 1339 3 ft 120V

1323* 1342* 1321* 1340* 3 ft 120V

1345 1305 1355 1325 4 ft 120V

1346 1306 1356 1326 4 ft 230V

1354 4 ft 230V, China

1384* 4 ft 230V, China

1370 1352 1368 1350 5 ft 120V

1371* 1353* 1369* 1351* 5 ft 120V

1347 1307 1357 1327 6 ft 120V

1348 1308 1358 1328 6 ft 230V

1359 6 ft 230V, China

1389* 6 ft 230V China

1332 1392 1330 1390 3 ft 230V

1333* 1393* 1331* 1391* 3 ft 230V

1375* 1335* 1385* 1395* 4 ft 120V

1376* 1336* 1386* 1396* 4 ft 230V

1382 1366 1380 1372 5 ft 230V

1383* 1367* 1381* 1373* 5 ft 230V

1377* 1337* 1387* 1397* 6 ft 120V

1378* 1338* 1388* 1398* 6 ft 230V

* Includes cabinet, adjustable height stand, UV light and armrest.

Thermo Scientificii 1300 Series A2

Preface

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance.

s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo Fisher Scientific be held liable for any damages, direct or incidental, arising
out of or related to the use of this manual.

©2011 Thermo Fisher Scientific. All rights reserved.

CAUTION

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

Thermo Scientific 1300 Series A2 iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.

WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal in
each EU Member State. For further information, send an email to [e-mail].

4 Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4 Always dissipate extreme cold or heat and wear protective clothing.

4 Always follow good hygiene practices.

4 Each individual is responsible for his or her own safety.

Thermo Scientificiv 1300 Series A2

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermofisher.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address

Certified Service Web Pagewww.unitylabservices.com

Our staff can provide information on pricing and give you quotations. We canSales Support

take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.

When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:

Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road, Box 649
Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

1300 Series A2 vThermo Scientific

Table of Contents

Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Safety Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Paper Catch Grids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

HEPA Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5

Use of the Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6

Unit Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8

Chamber Lighting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9

UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10

Work Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10

Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Optional Exhaust Transition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Exhaust System Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Moving the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4

Service Valve Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5

Universal Piping Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7

SmartPort . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8

Drain Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8

Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Assemble the Stand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Level the Cabinet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3

Unlock Counterweight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3

Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4

Installation Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5

Locating a Certifier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6

Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Unit Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3

Operating Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3

Loading the Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4

Working Recommendations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5

Error Codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5

Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7

Unit Shut-down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7

Procedure Interrupt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7

Section 1

Section 2

Section 3

Section 4

vi 1300 Series A2 Thermo Scientific

Cleaning / Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Care and Cleaning of Stainless Steel . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Cleaning and Caring for Coated Surfaces . . . . . . . . . . . . . . . . . . . . .5-2

Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

Rinsing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

UV Disinfection After Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

Change the UV Disinfection Time . . . . . . . . . . . . . . . . . . . . . . . . .5-4

Microbiological Space Decontamination . . . . . . . . . . . . . . . . . . . . . .5-4

Clean Exterior Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5

Clean Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5

Clean the Drain Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5

Clean the Paper Catch Grid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-6

Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Optional UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Sample Chamber Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3

Replacements and Repairs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3

Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Certification Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Classification of the Safety Cabinet . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Test Terms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2

Testing Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3

Test Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3

HEPA Filter Leak Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-9

Filters that cannot be Accessed or Scanned . . . . . . . . . . . . . . . . . . .8-9

Filters that can be Accessed and Scanned . . . . . . . . . . . . . . . . . . . .8-9

Site Installation Assessment Tests . . . . . . . . . . . . . . . . . . . . . . . . .8-10

Elect. Leakage , Ground Resistance, Polarity Tests . . . . . . . . . . . .8-10

Airflow Pattern Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-10

Data Log/Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Table of Contents

Section 5

Section 6

Section 8

Section 7

Section 9

1300 Series A2 1-1Thermo Scientific

Section 1 Description

The Thermo Scientific 1300 Series A2 offers innovative SmartFlow™
technology; an automatic airflow compensation system that adjusts motor
speed as filters load, without the use of a manual damper. The
SmartFlow™ system ensures safe working conditions, even between annual
certifications.

The plenum assembly consists of a plenum for the downflow blower and a
plenum for the exhaust air blower. Each blower includes a filter. The
exhaust air is discharged through an opening in the top of the cabinet.

Chamber lighting includes one fluorescent bulb for 4 foot models and two
for 3, 5 & 6 foot models.

The optional, factory-installed UV light is installed in the ceiling at the
front of the sample chamber.

Figure 1-1. Unit Components

RS-232

Connection

Fuseholders (2)

Exhaust opening

Power cord

Exhaust blower

Downflow blower

Plenum

access panel

Accessory

outlets

Smart Port

Supply

test hose

Armrest

Work
tray

Exhaust
test hose

Service
valve

Electrical enclosure

Plenum

Light dome

Control panel
Handle

Front window

Service valve

access ports

Stand (optional)

1-2 1300 Series A2 Thermo Scientific

Section 1

Description

Safety Systems

Three service valve access ports are located on each side panel. When
installing service valves, remove the center of the port plugs at the
perforations.

The optional stand may be ordered in two configurations; a manually
adjustable from 30” to 38” working height, and a fixed 34” working
height with attached casters.

A stainless steel one-piece work surface is standard. Optional armrests
ensure a comfortable working position for the user and minimize
blockage of airflow at the front grille.

Internal outlets, provided as the power source for accessories, are provided
standard in the following configuration:

1305, 1307, 1320, 1321, 1322, 1323, 1325, 1327, 1335, 1337, 1339,
1340, 1341, 1342, 1345, 1347, 1350, 1351, 1352, 1353, 1355, 1357,
1368, 1369, 1370, 1371, 1375, 1377, 1385, 1387, 1395, 1397: two
duplex right and left side
1306, 1308, 1326, 1328, 1330, 1331, 1332, 1333, 1336, 1338, 1346,
1348, 1358, 1359, 1366, 1367, 1372, 1373, 1376, 1378, 1380, 1381,
1382, 1383, 1388, 1389, 1390, 1391, 1392, 1393, 1396, 1398: two
single right and left side

Test hoses (aerosol challenge ports) are located under the work tray. The
supply plenum hose is located on the left and the exhaust plenum hose on
the right.

Note Do not remove the caps of the two test hoses to check for supply
and exhaust airflow.

s

Negative pressure air system

A negative pressure air system combines with HEPA filters in the sup­ply and exhaust airflow for personnel and product protection.

Personnel protection

Inflow air along the entire working opening at a constant high velocity
prevents leakage from the work opening of the chamber.

Exterior air pressure being higher than the internal air pressure creates
negative pressure, ensuring containment in case of cabinet leakage.

Product protection

Steady airflow within the air system ensures constant downflow, allow­ing the HEPA filters to remove contaminants so that the samples are
always surrounded by ultra-pure air. Harmful particles are not carried
over the sample chamber (protection from cross-contamination).

1300 Series A2 1-3Thermo Scientific

Section 1

Description

HEPA filters

The downflow air within the chamber and the exhaust air are cleaned by
HEPA filters.

Safety lockout

To protect from UV radiation, the optional UV light will not turn on if
the front window is open. While the UV light is on, the front window
should remain closed. The closed window blocks out the UV rays.
Opening the window will cause the UV light to turn off.

Digital Airflow Verification (DAVe)

Independent monitoring of inflow and downflow air velocities guaran­tees that product and personnel protection remain uncompromised.
Airflow monitoring determines the velocity of the airflow in the sample
chamber as well as the inflow velocity of air through the exhaust open­ing. As soon as airflow velocities rise above or fall below a specified safe­ty value, an audible and a visual alarm is activated.

Window position monitoring

The position sensors detect the size of the front window opening and
indicate whether the window is open to the specified work position,
closed (energy saving) or in an unsafe intermediate position.

SmartFlow Indicator

The SmartFlow Indicator provides assurance that the inflow is balanced
properly. SmartFlow monitors the advanced motor technology adjusting
airflows even as the filter resistances change due to loading or other
influences. The SmartFlow Indicator visually demonstrates the cabinet’s
ability to compensate to maintain personnel protection. Green indicators
show the fan and filters have power and capacity in reserve to respond to
additional demands. Yellow or red indicators show that while the cabinet
is providing personnel and product protection, maintenance should be
scheduled to restore the compensation reserve.

Safety Systems

(continued)

The filter system consists of two HEPA filters for downflow (supply) and
exhaust air.

Room air is drawn into the sample chamber through the work position
window opening. The room air is drawn into the negative pressure drain
pan air duct, then
pulled into the air
plenum in the top
portion of the cabinet.
The room air is mixed
with recirculating
HEPA filtered air from
the chamber, and
filtered proportionally
by the downflow and
exhaust filters. The
filtered air is then
supplied as ultra-pure
air into the sample
chamber of the unit,
and exhausted as ultra­pure air into the room
environment, or into
an external exhaust
system via an exhaust
transition.

Sectional paper catch grids are installed beneath the work tray in the air
duct between the inner and outer back wall of the unit. These grids
prevent large items such as paper towels and tissues from entering the
plenum where they may impair the function of the blowers or the filters.
The grids can be easily removed for cleaning.

1-4 1300 Series A2 Thermo Scientific

Section 1

Description

HEPA Filters

Figure 1-2. Airflow Filtering System

Paper Catch Grids

Ultrapure air

Exhaust air filter

Exhaust blower

Room air

Supply blower

Supply filter

Ultrapure air

Downflow
in chamber

Blended air

Paper catch
screens

1300 Series A2 1-5Thermo Scientific

Section 1

Description

The control panel is located on the front of the cabinet and consists of
keys, status indicators and a numerical display.

The display with its 5-digit panel shows the following information,
regardless of the activated operating function:

• filter life meter (reset on filter replacement or attaining 10,000 hours)

• downflow and inflow air velocities

• remaining time of the UV disinfection routine

The keys turn functions on or off.

Status indicators show:

• window work position

• airflow

• reduced blower speed

• SmartFlow Indicator

Control Panel

Figure 1-3. Control Panel

Keys

Display

ft/min

SMARTFLOW INDICATOR

Status Indicators

The sample chamber is accessible through various positions of the front
window. The working position of the window is a 8 or 10 inch opening,
+0.25”, -0.75” (depending on model - check data label and first page of
this manual). For loading the chamber, the window opens fully. For
decontamination and energy saving, enter into Night-Set-Back mode by
closing the window. When the window is closed, the blower speed is
reduced, minimizing energy consumption and noise emittance without
compromising particulate containment inside the work chamber.

Note The working and loading window positions are indicated on the user
interface, as controlled by micro switches and displayed by status
indicators on the control panel.

s

Use of the Window

1-6 1300 Series A2 Thermo Scientific

Section 1

Description

Figure 1-4. Window Openings

Window

Max opening for
loading samples

1300SERIES A2

Work
position

Closed position

Window

Opening height

for cleaning window

Pillar dress

panel

1300SERIES A2

Pillar dress

panel

To place the window at the work position, move it to the area of the top
‘dimple’ (Figure 1-5) and watch for the GREEN sash position height
indicator to illuminate (Figure 1-6). If the window is moved from the the
work position, the sash height indicator will turn RED.

To place the window in the closed or reduced mode position, move it to
the area of the lower ‘dimple’ (Figure 1-7) and watch for the BLUE
reduced mode indicator to illuminate (Figure1-8). If the window is not
positioned correctly, or is moved from the closed position, the BLUE
indicator will be extinguished. When the BLUE reduced mode indicator
is not illuminated, the unit will not operate in reduced mode, and the
optional UV light will not function.

1300 Series A2 1-7Thermo Scientific

Section 1

Description

Use of the Window

(continued)

Figure 1-5. Work Position Figure 1-6. Lighted Green LED

Figure 1-7. Fully Closed Position (UV, if applicable) Figure 1-8. Lighted Blue LED

dimple

dimple

1-8 1300 Series A2 Thermo Scientific

Section 1

Description

Our patented-pending SmartClean™ window design easily lowers below
the work position for replacing the sample chamber light bulbs.

Caution If the front window jams or is sluggish to move, do not use force!
Contact Technical Services immediately. Do not attempt to repair this
problem.

s

The standard unit includes accessory outlets, service valve access ports, an
RS-232 connection and a remote alarm connection.

The power cord to be connected to the power source is located on the top,
near the front of the cabinet.

The accessory outlets (max 5A) are located on the interior chamber back
wall.

On top of the cabinet are the two fuseholders for the 5A accessory outlet
fuses and an RS-232 connection (signal source, not customer interface).

Service valve access ports are located on each side of the cabinet. To use
these ports, remove the center perforation. Service valves are available as
optional accessories.

The remote alarm accessory can be used for notification of window
movement or access.

Warning If a gas burner is to be operated in the sample chamber, an
appropriate shut-off valve for the gas supply must be installed. Use only
laboratory safe burners in the sample chamber.

s

Use of the Window

(continued)

Unit Interface

1300 Series A2 1-9Thermo Scientific

Section 1

Description

The Thermo Scientific 1300 Series A2 offers a bright workspace for a
more comfortable working environment. Models with coated rear and
side walls resist glare and make it easier for the user to work safely.

Lighting for the sample chamber includes one fluorescent bulb for 4 foot
units, and two bulbs for 3, 5 & 6 foot units. The bulb(s) is installed
behind the light canopy.

Figure 1-9. Interfaces (2 of 4 access ports are shown)

Chamber Lighting

Unit Interface

(continued)

L

Fuses

N

RS-232

Power cord

Accessory outlet
(1 of 2 shown)

Access
ports

Service
valve

1-10 1300 Series A2 Thermo Scientific

Section 1

Description

The optional, factory­installed UV light is
installed at the top of the
front section of the sample
chamber.

The operating time of the
UV light is preset for 1
hour. The UV disinfection
procedure can be started by
pressing the “UV” key on
the control panel with the
window in the closed
position. If the window is
opened, the procedure is
immediately cancelled.

Note To protect from UV radiation, the UV light can only be turned on
when the window is completely closed.

s

The single piece work tray is provided with the cabinet. It is placed onto
the frame above the sample chamber drain pan. It can be removed by
lifting up, using the large holes in the work tray.

The working area for optimal product protection extends over the entire
width and depth of the work tray. The two optional armrests are
positioned in the center of the working area 8 inches (20cm) from each
other. To attach the armrests, insert into the first perforated track of the
work tray.

Warning Work safety is assured only if the armrests are used correctly!

s

Work Area

UV Lights

Figure 1-10. Lights

Figure 1-11. Work tray

UV light

Chamber light

Work tray

Perforation

track

Armrest

Separation
distance

Armrest

Width

Depth

Perforation
track

1300 Series A2 2-1Thermo Scientific

Section 2 Installation

The operational safety and proper function of the unit depend on the
location where it is to be operated. The cabinet should be operated only in
the ambient conditions listed below.

• Up to 6562 ft. (2000m) above sea level.

• Power outlet accessible to authorized personnel only. Ideally, the outlet

should be installed above the biological safety cabinet. The power cord
is the mains disconnect.

• Location capable of supporting the weight of the device and non-

flammable.

• If the factory-approved stand is not purchased, the holding device must

be rated for twice the cabinet weight.

• Adequate room height. For devices that are not connected to an

exhaust system, the distance between the exhaust air opening at the
device and the room ceiling must be at least 8 inches (200mm).

• Equipped with an appropriate ventilation system.

• Room temperature between 10°C and 40°C (50°F and 104°F).

• Relative humidity must not exceed 80%, up to 31°C and decreasing

linearly to 50% at 40°C.

• For indoor use only.

Room ventilation should preferably be a ventilation system that complies
with the national requirements for the application.

The inlet air and exhaust air openings of the room ventilation must be
located so that drafts do not impair the function of the biological safety
cabinet air system.

When positioning the cabinet, make sure the counterweight on the back of
the unit can move freely. The minimal distance to the wall or adjacent
objects should be 3 inches (7.6cm), unless upper wall brackets are used.

The part numbers for the optional thimble exhaust connection are
1911316 for three, four & five foot units, and 1911317 for six foot units.

The test conditions according to NSF/ANSI 49 were performed with the
standard version of the safety cabinet, without exhaust system accessories.

An available accessory for the biological safety cabinet exhaust system is an
exhaust thimble connection for an on-site exhaust system with blower.
According to NSF/ANSI Standard 49, an exhaust alarm is required with
this connection.

The exhaust system components are installed to the exhaust opening on
top of the cabinet. The threaded inserts for the retaining screws are pre­installed in the unit.

Installation on 3, 4 and 5 ft. models:

1. Turn the unit off and disconnect
it from the power source.

2. Thoroughly clean the surfaces
around the exhaust opening and
of the accessory to be installed, to
ensure that they are free of debris.

3. Secure the accessory by tightening
the supplied retaining screws
(M5) wrench-tight.

4. Using the adjustment nuts on the
sliding panel, set the gap height
to an opening of 2 inches.

2-2 1300 Series A2 Thermo Scientific

Section 2

Installation

Optional Exhaust

Transition

Exhaust System

Accessories

Figure 2-1. 4 ft Thimble

Thimble

Retaining
screws

Exhaust
opening

Threaded
inserts

The exhaust thimble connection is installed to the top of the cabinet using
an additional adapter. The threaded inserts for the retaining screws of the
adapter are pre-installed to the top of the cabinet.

Installation on a 6 ft. model:

1. Turn the unit off and disconnect it from the power source.

2. Clean the contact surfaces around the exhaust opening, and the upper
and lower contact surfaces of the adapter thoroughly to ensure that all
grease and dust residue are removed.

3. Secure the adapter to the pre-installed threaded inserts on top of the
cabinet using the supplied four retaining screws. Tighten the screws
hand-tight.

4. Secure the accessory by
tightening the supplied
retaining screws (M 5)
wrench-tight.

5. Using the adjustment nuts
on the sliding panel, set
the gap height to an
opening of 2 inches.

1. Place the connecting
sleeve onto the connecting
opening of the accessory
and secure it with the
screws.

2. Fit the connecting pipe
(200mm [8 in]) of the on­site exhaust air system to
the connecting sleeve.

3. Seal the unused
connecting opening with
the cover. Place the cover
onto the connecting
opening at the housing
and secure it with the
screws.

1300 Series A2 2-3Thermo Scientific

Section 2

Installation

Connecting Sleeve

Exhaust System

Accessories (continued)

Figure 2-2. 6 ft Thimble and Sleeve

Thimble

Retaining
screws

Exhaust
opening

Threaded
inserts

Adapter

Pipe

Sleeve

Cover

Sleeve

Opening

2-4 1300 Series A2 Thermo Scientific

Section 2

Installation

Moving the Unit

To prevent tilting, always transport the cabinet separate from the stand,
using equipment capable of supporting the weight, even for a move within
a building.

Caution When moving the unit, lift only at the lift points shown in Figure
2-3.

s

Caution Do not allow the weight of the cabinet to rest on the drain pan! s

Warning The weight of the window is balanced by the counterweight on

the back of the cabinet. Do not move the unit unless the counterweight
has been locked in place with the shipping screws (see Figure 3-2). s

Figure 2-3. Lift points

Install the service valve(s) by
removing the centers of the plug
(brass) or piercing it at the pre­determined location (plastic).

Installation spacing on side of
unit is indicated in Figure 2-4.

Note The plugs must only be
used for the installation of service
valves in accordance with the
applicable national regulations.

s

At each side panel, service valves can be installed through the three service
valve port plugs (P/N 249095-exterior port plug, 249096-interior port
plug).

1. Move the front window to the fully open position.

2. Turn the unit off and disconnect it from the power source.

3. Score the plug circularly at the pre-determined point (inside and
outside). Remove the centers.

4. Referring to Figure 2-5 on the following page, slide the bezel onto the
threaded pipe of the service valve. Apply a thin bead of silicon to the
inner flange of the service valve.

5. From inside the sample chamber, slide the service valve through the
desired plug.

6. From the outside, slide the washer onto the service valve threaded pipe
and apply a thin bead of silicon to the outer flange of the service valve.

7. Secure the service valve to the side panel using the nut.

8. Establish the connection to the supply line using a union nut.

Note If a service valve is removed, the service valve port plug should be
re-installed.

s

1300 Series A2 2-5Thermo Scientific

Section 2

Installation

Service Valve

Connections

Figure 2-4. Valve Location

SmartPort

2-6 1300 Series A2 Thermo Scientific

Section 2

Installation

Valve Connections

(continued)

Figure 2-5. Valve Assembly and Installation

If SmartPorts are installed, replace the SmartPort grommet before starting
a decontamination procedure. Two replacements (P/N 114111) are
shipped with each unit, or the 1910112 kit containing 4 grommets can be
ordered.

SmartPort locations are indicated above (one on each side).

Replace the SmartPort

™

Before Decontamination

Smart Port

Service valve

Service
valve

Bezel

Threaded

pipe

(3/8”MPT)

Washer

Service valve
port plugs

Union

nut

Nut

Universal piping is a factory-installed option. The piping can be installed
on either, or both, sides. Access points, depending on selected installation,
are available on the top, side or bottom of the cabinet. Pipe threads are
1/4” FPT. Refer to illustration below.

Note The top left access point is not available on 3 cu ft units, due to the
proximity of the exhaust filter. On 3 cu ft units, the right side access
points are next to the right side rope channel. The left side access points
are to the left of these.

s

1300 Series A2 2-7Thermo Scientific

Section 2

Installation

Universal Piping

Connections

Figure 2-6. Possible Universal Piping Access Points

top access points

3 cu ft units only

left side

access points

bottom of cabinet
access points

right side
access points

bottom of cabinet
access points

The SmartPort is an optional feature on the 1300 Series A2, designed to
improve work area organization inside the biological safety cabinet. It is
designed to accommodate the routing of vacuum line tubing, line cords
and data cables from the interior of the cabinet to the exterior working
area, freeing up space inside the work area.

A 3 inch access port with an inner and outer solid rubber grommet is
located on both side walls of the cabinet. Soap bubble leak test should be
performed while the grommet is closed. Using a surgical scalpel, the user
may cut the inner and outer grommet along the pre-cut indentations,
cutting only what it needed to accommodate the tubing or cable. The
negative pressure air channel within the side walls ensures that no particles
escape into the lab.

At any time, new solid rubber grommets may be installed into the
SmartPort to provide a complete seal in the side walls. It is recommended
that new solid grommets be installed on the exterior side walls, prior to
decontaminating the cabinet. Two new solid grommets arre supplied with
the cabinet for this purpose, or kit # 1910112 (four grommets) can be
ordered.

After the unit has been installed, either on a stand or a workstation, the
drain valve should be installed. The drain valve itself is shipped in the
manual bag taped to the inside of the unit.

Locate the drain piping underneath the unit on the left side. Apply thread
sealant sparingly to the threads.

Install the drain valve. Ensure the valve is in the closed position.

Be aware of the valve when moving the unit from the stand or
workstation.

2-8 Model 1300 Series A2 Thermo Scientific

Section 2

Installation

SmartPort

Drain Valve

1300 Series A2 3-1Thermo Scientific

Section 3 Start-Up

Caution Correct assembly and installation is essential for proper start-up

and operation. s

For a unit without a stand, place the cabinet onto a stable structure
capable of supporting two times the unit weight. Make sure the weight is
not resting on the drain valve or nipple.

For a unit with a stand, first assemble the stand per the instructions
included with the stand. Then fit the cabinet onto the stand. See steps
below.

Caution Make sure each leveler is turned all the way in, to avoid bending
an edge during installation.

s

1. Locate the four telescoping legs in the side panel packaging. Install
adjustable leveler from the hardware bag into the swaged end of the
telescoping legs (if not already installed). NOTE: Make sure leveler is
screwed all the way in, before installation. Using a hammer, tap the
leveler into place.

2. Locate the left and right side panels. Install the telescoping leg
assemblies into the side panels as shown in Figure 3-1.

3. The height-adjustable stand can be set to a height range of 26.8 - 34.7
in. (680 - 880mm ). To set the stand to the desired height, pull the
legs downward out of the channels to the desired height. Lock the
height adjustment in place by installing two lockwashers/screws per
channel to the leg.

Note All lockwashers are a wedgelock type. The wedges fit together with
the smoother sides toward the unit and the screw. See Detail.

s

4. Locate the two crossmembers from the packaging. Note they are
packaged separately from the side panels. With the label on the
crossmember to the outside (Figure 3-1), install the crossmembers onto
the retaining tabs of the side panels. Place lockwashers on screws, then
install all screws by hand, before tightening to secure together.

5. To install the cabinet frame to the stand, insert four flatwashers and
Allen screws loosely into the threaded holes at the bottom of the
cabinet.

Assemble the Stand

3-2 1300 Series A2 Thermo Scientific

Section 3

Start-Up

Figure 3-1. Stand Assembly

Assemble the Stand

(continued)

6. Place the safety cabinet onto the stand so that the Allen screws are
routed through the holes of the keyhole tabs.

7. Slide the cabinet frame to the keyhole stop (Fig. 3-1).

8. Tighten the four Allen screws.

9. Level the cabinet (instructions follow in this section).

Detail. Order of Assembly

Channel

Leg

Lockwasher

Lockwasher

Screw

Screw

Cabinet

Side
panel

Crossmembers

Crossmember

Keyhole

Stand

Retaining tab

Side

Screws

Back

Lockwashers

Telescoping leg

Leveler

Screws

Cabinet

front

Screw with

flatwasher

Keyhole

Keyhole

tab

Foot

Keyhole

stop

1300 Series A2 3-3Thermo Scientific

Section 3

Start-Up

Unlock

Counterweight

The window counterweight is secured to the back of the cabinet to protect
the window from damage during shipping.

Caution The counterweight on
the cabinet back compensates for
the weight of the front window.
Do not try to move the front
window before the counterweight
has been unlocked.

s

Warning After unlocking, keep
hands and fingers away from the
motion range of the
counterweight!

s

To unlock the counterweight,
remove the shipping screws (see
location arrows in Figure 3-2)
and retain them for future
shipping needs. A Torx wrench is shipped in the manual bag for this
purpose.

Warning One person should hold the counterweight while a second
person removes the screws. This facilitates screw removal and avoids the
possibility of stripping the screws.

s

Note The cabinet should be leveled only after it has been located in the
desired working location.

s

For cabinets without a stand, place a bubble level onto the work tray
surface and adjust the cabinet until the level indicates an absolutely
horizontal position in all directions.

For cabinets with an optional stand, place a bubble level onto the work
tray and adjust the four levelers of the stand using an open end or
adjustable wrench until the bubble level indicates an absolutely horizontal
position in all directions. Check from right to left, and from back to front.

Level the Cabinet

Figure 3-2. Unlocking the Counterweight

Counterweight

Warning Contact with electrical components can cause a lethal shock.

Warning Before connecting the unit to a power source, check plug and

power cord for possible damage. Do not use damaged components to
connect the unit to the power source!

s

1. Before connecting the unit to the power source, verify that the voltage
of the outlet matches the specifications on the cabinet nameplate. If the
ratings given for voltage (V) and maximum current (A) do not match,
do not connect the unit to the power source.

2. Connect the power cord to a properly grounded and fused outlet.
The outlet must be fused separately, using a fusible link T15A or using
a circuit breaker B15. If the configuration of the receptacle does not
match the power plug, consult a qualified electrician to replace or
install the correct receptacle.

3. Make sure that the power cord is routed away from the counterweight
and cable guide. The left side of the cabinet has additional fastening
points that can be used for routing the power cord.

4. Make sure that the power cord is not stretched or pinched.

To protect from accidental disconnection, the power source outlets should
be located out of casual reach and be accessible only to authorized persons.
Ideally, the outlet should be located above the cabinet to avoid accidental
shut-off.

The power supply cord is the mains disconnect. Maintain access at all
times.

If the unit includes service valves (gas, water), the valves must be installed
properly to ground, to the unit’s main ground.

On connection to the power source, place the window at the working
position. Press and hold the On button. A green LED in the display
indicates power is being supplied to the cabinet. The cabinet is now ready
for operation and can be operated using the control panel.

3-4 1300 Series A2 Thermo Scientific

Section 3

Start-Up

Power Connection

Warning Do not operate the unit before initial operation, installation and

certification tests have been performed.

s

The installation test must be performed in accordance with the
specifications of NSF/ANSI 49. The cabinet may be operated as a Class II
biological safety cabinet, in accordance with NSF/ANSI 49, if the unit
functions listed below were verified and if the test results are within the
safety value tolerances as specified in NSF/ANSI 49, Annex F.

• Inflow velocity profile test

• Downflow velocity profile test

• HEPA filter leakage test

• Airflow smoke pattern test

• Site installation assessment tests

• A repeat test must also be performed annually, after repairs to the unit
or after location changes.

• The operator should request a written test report from the authorized
service technician.

Warning The operational safety of the unit, particularly the personnel and
product protection, are guaranteed only if all safety functions of the unit
have been tested and approved.

s

Caution Thermo will not warrant operational safety if the unit is operated
without the required installation and certification test, or if these tests and
repeat test are not performed by adequately trained and authorized
personnel.

s

Caution The initial operation with subsequent installation test does not
include any decontamination measures. The sample chamber and any
accessories required must be disinfected and cleaned in accordance with
the hygiene guidelines set forth for the desired application. s

Caution Alarms on this unit are not factory-set. Set the alarms to avoid
product loss and ensure personnel safety. Refer to Certification section.

s

1300 Series A2 3-5Thermo Scientific

Section 3

Start-Up

Installation Tests

Caution Service and certification must be performed by qualified

personnel. s

Biological safety cabinet certification consists of a series of tests designed to
verify that the cabinet is performing within operating parameters
established by the manufacturer. To assure that a biological safety cabinet
is operating as intended, each cabinet should be field-tested at the time of
installation and at least annually thereafter. Cabinets should be re-certified
whenever HEPA filters are changed, internal maintenance is performed, or
the unit is relocated.

Three industry-related organizations maintain lists of companies and
individuals who are active in the certification industry. You may contact
these organizations at the addresses listed below.

NSF International (NSF) and International Air Filtration Certifiers
Association (IAFCA) sponsor certifier accreditation programs. Accredited
certifiers have demonstrated proficiency at testing biological safety cabinets
by successfully completing written and/or practical examinations.

Biohazard Cabinet Field Certifier Program

NSF International

PO Box 130140

789 N. Dixboro Rd

Ann Arbor, MI 48113-0140

Telephone [phone] Or (800) NSF-MARK

Fax [phone]

http://www.nsf.org/Certified/Biohazard-Certifier

IAFCA

PO Box 12155

Columbus, OH 43212

Telephone [phone]

Fax [phone]

http://www.iafca.com/certifier.html

3-6 1300 Series A2 Thermo Scientific

Section 3

Start-Up

Locating a Certifier

The Controlled Environment Testing Association (CETA) is a trade
association devoted to promoting and developing quality assurance within
the controlled environment testing industry. A list of active members is
available by contacting the organization.

Controlled Environment Testing Association

1500 Sunday Drive

Suite 102

Raleigh, NC 27607

Telephone [phone]

Fax [phone]

http://www.cetainternational.org/members/corp_indiv.htm

Note Unless certification was expressly called for in the specification,
quotes and/or purchase order, the cost for this on-site testing is to be paid
for by the customer.

s

1300 Series A2 3-7Thermo Scientific

Section 2

Start-Up

Locating a Certifier

(continued)

1300 Series A2 4-1Thermo Scientific

Section 4 Operation

The control panel has been divided into three functions; display, keys,
status indicators.

The display shows the information below during each operation.

• during normal operation, the operating hours of the unit,

• during safe work mode, the downflow and inflow*,

• with UV light (optional) timer on, the remaining disinfection time.

*Press the Hours/Velocities key to switch between the operating hours,
and the downflow and inflow in safe work mode (with window in the
open, safe operating work position).

Figure 4-1. Control Panel Functions

Display

Power

Indicator

Green

Hours/
Air velocities

On/Off

SMARTFLOW INDICATOR

Green

Yellow

ow

Yell

ft/min

Red

Light
On/Off

Power
LED

Red

Window
in work

position
(green)

Outlets
On/Off

LED

Window

NOT in

work

position

(red)

UV Light
On/Off

UV

Airflow

steady
green)

Silence

Airflow

NOT

steady

(red)

Reduced
blower speed/
window closed/
UV interlock
indicator

4-2 1300 Series A2 Thermo Scientific

Section 4

Operation

To enable (I) / disable (0) alarm ringback function, follow the steps below.

In OFF mode, press and hold the Hours/Air Velocities key for
approximately five seconds. The display momentarily shows the operating
hours, then the switching state: I = alarm acknowledge function enabled,

0 = alarm acknowledge function disabled.

Light key turns the sample chamber light on and off.

The On/Off key turns power to the unit on or off.

Outlet On/Off key turns power to the internal outlets on and off (the blue
LED indicates that voltage is present).

Note Many of our units are equipped with a Ground Fault Circuit
Interrupter (GFCI) on the left side of the cabinet. This device monitors

the amount of current flowing from hot to neutral. If there is an
imbalance, it trips the circuit. This single GFCI protects both outlets

within the cabinet.

If the outlets do not operate when the power button is turned on, press
the Reset button in the center of the GFCI outlet to ensure the GFCI is
set and power is flowing through it. If this does not restore power, contact
Technical Services for additional troubleshooting.

UV Light key turns the optional UV light on and off. The yellow LED
indicates the UV light has been activated. If the UV light is not installed,
this key has no function.

This key also activates the UV disinfection procedure. During the
procedure, the display alternates between dIS and the remaining preset
time (see Section 5 to change this preset).

Silence key for muting the audible alarm.

Figure 4-2. Reset Button on GFCI Outlet

1300 Series A2 4-3Thermo Scientific

Section 4

Operation

1. To turn the unit on, press and hold the ON key until the blowers start
(audible blower operation), status indicators (LEDs) illuminate, and an
audible tone sounds. Note A delay of several seconds may occur
between key actuation and unit response.

s

2. Move the window to the work position (8 or 10 inch opening, model
dependent - see data label and first page of this manual). The correct
position is reached when the green LED “front window is in work
position” illuminates. The side guide rails have markings that define
the lower edge of the front window. These markings can be used as an
additional orientation aid.

3. Wait until the green LED “airflow is steady” illuminates.

4. The unit is ready for operation.

The following are the operating modes for this unit.

• OFF mode • Window open mode

• Working mode • Standby mode

• UV mode

Off mode means the unit is in an idle state. The unit is turned off
(blowers are off). The sample chamber light is enabled. The unit is
connected to a power source. The power supply for the internal outlets is
enabled when the blue LED “internal power supply on” is lighted.

Window open mode is the state in which the window is open and
positioned outside of the working position (8 or 10 inches, model
dependent - see data label and first page of this manual). To install or
remove accessories, the front window can be moved to the maximum open
position. For cleaning, the front window can be lowered beyond the closed
position (see Use of the Window, Section 1).

The air system blowers are switched on with the red LED “airflow not
steady” lighted. The front window is not in the work position, with the
red LED “front window not in work position” is lighted. The sample
chamber light is enabled. The power supply for the internal outlets is
enabled when the blue LED “internal power supply on” is lighted.

Working mode is active when the front window is in the working position
and the air system is operating steadily. The green LEDs “front window is
in work position” and “airflow steady” are lighted.

The markings on the side guide rails and the lower edge of the front
window are at the same height with no audible alarm signal.

Unit Start-Up

Operating Modes

The sample chamber light is enabled and power for the internal outlets is
enabled when the blue LED “internal power supply on” is lighted.

Standby mode is the state in which the window is closed with the blower
velocity reduced. The red LED “front window not in work position”, red
LED “airflow not steady” and the blue LED “airflow reduced” are lighted.

The sample chamber illumination is enabled. The internal power supply in
the sample chamber is enabled when the blue LED “internal power supply
on” is lighted.

The blowers on the biological safety cabinet can be turned off (OFF mode)
by pressing and holding the ON key for five seconds until all LEDs are off.

UV mode (lighted yellow LED) means the UV light is on with a set timer
running. The window must be in the closed position to protect against UV
radiation. The light will not turn on with the window open.

The yellow LED “UV disinfection routine activated” is lighted until the
preset time has elapsed. Then, the UV lights turn off automatically, and
the status indicator turns off.

The sample chamber illumination is disabled in this mode, as well as the
internal power source in the sample chamber.

1. Move the front window to the maximum opening position. This
automatically switches the blowers to full speed.

2. Install needed work materials within the sample chamber work area.
Avoid blocking the front air intake grille.

3. Move the front window to the work position (the green status
indicator Front Window in Work position is illuminated) and wait
until the airflow has stabilized (the green status indicator Airflow
steady is illuminated).

Warning Personal and product protection is ensured only if the airflow
system of the device is working properly. If the alarm system issues a
failure message for more than a few minutes while the front window is in
work position, stop all applications that may jeopardize worker safety.

s

4. Load the work tray with samples.

5. For work breaks or for extended experimental phases without manual
intervention, switch the device to standby mode by closing the
window.

4-4 1300 Series A2 Thermo Scientific

Section 4

Operation

Operating Modes

(continued)

Loading the Chamber

Failure messages are shown on the display as error codes ER 3 to ER 7. If
one of these codes appears on the display, contact Technical Services
immediately.

To isolate the cause of the failure, perform the following tests.

• Check to see if the exhaust opening on top of the cabinet is blocked.

• Ensure that the in-house exhaust air system is activated, if applicable .

• Close doors and windows in the laboratory to prevent drafts.

• Turn off devices in the vicinity of the biological safety cabinet that
cause air turbulence, or emit excessive heat.

• Open flames in the sample chamber may impair airflow conditions.

1300 Series A2 4-5Thermo Scientific

Section 4

Operation

Error Codes

The observance of work rules ensures a minimum of operational safety
when handling the biological safety cabinet.

Before starting a procedure, take off all jewelry, put on required personal
protective equipment (gloves, goggles, apron), and clean and disinfect the
sample chamber surfaces at regular intervals.

During operation:

• Place samples only within the defined work area of the work tray.

• Do not place unnecessary items into the sample chamber.

• Use only disinfected and cleaned accessories for the work process.

• Do not cause air turbulence by quick hand, arm or body movements
in the sample chamber or in front of the work opening.

• Do not place accessories into the sample chamber that cause air
turbulence or emit excessive heat.

• Do not block air circulation at the ventilation slots of the work tray.

Working

Recommendations

• A height-adjustable working chair with an adjustable seat back should
be used during extended work periods at the cabinet.

• When the forearm rests on the armrest, is should be in a nearly
horizontal position.

• When the upper leg is in a horizontal position, the angle between
upper and lower leg should not exceed 90°.

• To compensate between the floor and sitting height, a footrest should
be used. The minimal effective size of the footrest should be 18 x 14 in
(45 x 35cm). The slope should be adjustable to within a range from 5°
to 15°. The adjustable height should extend to a minimum of 4 in
(11cm) above the floor.

After completing a procedure,

1. Remove samples from the sample chamber and store them properly.

2. Clean and disinfect the sample chamber surfaces, including the work
tray and the drain pan.

3. Clean and disinfect all accessories.

Working (continued )

4-6 1300 Series A2 Thermo Scientific

Section 4

Operation

Figure 4-3. Sitting Posture

Forearm
on armrest
horizontal

Upper leg horizontal

90° between upper

and lower leg

Slope 5° to 15°

Minimum 4 inches

off floor

15°

1300 Series A2 4-7Thermo Scientific

Section 4

Operation

Procedure Interrupt

To interrupt a work process:

1. Remove all samples from the chamber and store them properly.

2. Remove any accessories from the chamber, and clean and disinfect
them.

3. Clean and disinfect the chamber surfaces, work tray, and drain pan.

4. Turn the unit to standby mode by first closing the window. Then press
and hold the ON key until the indicators are off (the right indicator
segment of the display shows a dot to indicate that voltage is present).

Caution For safety reasons, the blowers can be switched off only when the
front window is closed.

s

If the device is not to be used or is to be stored for an extended period of
time, it must be completely decontaminated.

Warning To shut the device down, the sample chamber must be
disinfected completely and the plenum, including the filters, must be
decontaminated using the appropriate and authorized procedures.

s

1. After the device has been decontaminated, close the window
completely.

2. Disconnect the unit from the power source.

All components with the exception of the HEPA filters can be discarded
after having been thoroughly cleaned and decontaminated. The HEPA
filters must be discarded in accordance with the applicable national, state
and local regulations for special solid waste.

Warning As this unit can be used for processing and treating infectious
substances, it must be decontaminated prior to disposal, in accordance
with acceptable standards and procedures.

Unit Shut-down

Unit Disposal

1300 Series A2 5-1Thermo Scientific

Section 5 Cleaning / Decontamination

Several procedures can be used for decontaminating the biological safety
cabinet. Which procedure is selected, depends on the potential risk
imminent in the agents used, and/or the degree of cleanliness required by
an experiment or work process.

One possible decontamination procedure is to clean, rinse and dry with a
disinfection liquid or spray, and sterile distilled water.

UV disinfection can also be used. It is particularly suited as a more intense
disinfection after the above procedure.

Autoclaving (sterilization with steam) can be used for treating the
removable stainless steel components. The work tray(s), armrests and paper
catch grids are autoclavable components.

Disinfection with formaldehyde can be performed if a sterile sample
chamber is required for the work procedure. This sterilization procedure is
mandatory before filters are replaced, or before the unit is discarded.

Stainless steel is a combination of many different metals including iron and
chromium. Iron, the primary element in stainless steel, tends to corrode
(rust) when in its natural state. The chromium content in stainless steel
prevents corrosion.

Stainless steel is not corrosion or rust proof, just resistant to stains, or
stains “less”. The chromium, in the presence of oxygen, forms a tough,
invisible, passive layer of chromium oxide film on the steel surface. If
damaged mechanically or chemically, this film is self-healing as long as it
has enough oxygen. The presence of any liquid or solid that remains in
contact with the stainless steel for a prolonged time can prevent oxygen
contact and promote corrosion, as can prolonged contact with cleaners or
disinfectants containing chlorine, ammonia, iodine or other caustic agents.

Care and Cleaning of

Stainless Steel

5-2 1300 Series A2 Thermo Scientific

Section 5

Cleaning and Decontamination

Cleaning/Caring for

Stainless Steel (cont.)

Cleaning and Caring

for Coated Surfaces

1. To properly care for stainless steel, use cleaners and disinfectants free of
caustic agents such as chlorine, iodine and ammonia.

2. Always follow the application of any cleaner or disinfectant with a
minimum of two clean distilled water rinses, then a thorough drying
with a clean soft cloth. By rinsing with distilled water and drying, any
remaining residue is removed from contact with the stainless steel.

Caution Never use abrasive cleaners, scouring pads or steel wool when
caring for stainless steel.

s

If the stainless steel does become stained, corroded or rusted, the iron

deposits left on the surface can be removed by neutralizing them with
the passivation process. This process uses an acid to neutralize the
“free iron” deposits left on the steel where there has been deprivation
of oxygen. This stops the spread of the corrosion or rust. It will not
return the stainless steel to its original finish.

All the coated surfaces of this equipment are powder coated. Powder
coating is a method of applying a dry powder to electro-statically charged
metal, then baked in an oven where the dry powder molecules are melted
and fused together. This is by far the most durable finish available today
and, if cared for properly, will last for many years. The coated surfaces
should be cleaned with a neutral detergent and rinsed twice with clean
distilled water, then dried thoroughly with a clean soft cloth. Cleaning or
disinfecting coated surfaces without rinsing with clean distilled water and
drying thoroughly will result in smearing, streaking and dulling of the
coated surfaces. Never use abrasive cleaners, scouring pads or steel wool.
If the coated surfaces do become dull, streaked, smeared or marred in some
other way, there is no known method to restore the finish.

1. Remove all samples from the chamber and store them properly.

2. Remove accessories from the cabinet and disinfect them using the
disinfection procedure recommended by the manufacturer of the
accessory.

3. The work tray(s) and stainless steel components can be removed from
the chamber and disinfected separately.

4. Clean all chamber surfaces with disinfectant.

5. Do not remove the optional UV lights from the sockets. Clean
thoroughly with a damp cloth. Do not saturate. Be sure to dry
completely.

1. Rinse all surfaces twice with clean distilled water.

2. Discard any liquid from the drain pan. Rinse pan.

3. Dry all chamber surfaces completely.

UV disinfection can be performed by using the optional factory installed
UV light.

To start the UV disinfection procedure,

1. Close the front window, the air system operates in the reduced mode
(blue LED illuminated).

2. Press the UV key on the control panel. The display alternates between
dIS and the remaining disinfection time.

To interrupt or cancel the UV disinfection procedure, just press the UV
key (the display shows the operating hours) and slide the window up.

Rinsing

Disinfection

1300 Series A2 5-3Thermo Scientific

Section 5

Cleaning and Decontamination

UV Disinfection After

Cleaning

Microbiological Space

Decontamination

5-4 1300 Series A2 Thermo Scientific

Section 5

Cleaning and Decontamination

The UV disinfection time is preset. However, this setting can be changed,
if required.

1. Turn the unit on and move the window to the work position.

2. Press and hold the UV key until the preset time is displayed.

3. To increase the time, press the internal power supply key. Each time
the key is pressed, the time increases by 30 minutes.

4. To reduce the time, press the sample chamber light key. Each time the
key is pressed, reduces the time by 30 minutes.

5. To save the value, press the UV key. The display shows the operating
hours again.

Note The UV disinfection time is adjustable from 0:00 (disabled) to 24:00
hours.

s

NSF/ANSI 49, Annex G recommends a microbiological space
decontamination when maintenance work, filter changes, and performance
tests require access to any contaminated portion of the cabinet and before
the BSC is moved to another location. A microbiological space
decontamination may also be required by the BSC owner or user before
certification or as a part of their work procedures. NSF/ANSI 49 provides
recommended procedures for microbiological space decontamination using
formaldehyde and chlorine dioxide. NSF/ANSI 49 also recognizes that
alternative methods including the use of vaporized hydrogen peroxide are
sometimes validated and approved by BSC owners and users. The
appropriate method and procedure should be reviewed and approved by
interested parties including the BSC user, institution biological safety
officer, and BSC certification and service personnel as appropriate.

Warning Decontamination with formaldehyde and chlorine dioxide must
be performed in accordance with the specifications of NSF/ANSI 49,
Annex G. As this procedure has considerable risks, it must only be
performed by specially trained and authorized service personnel! Before
Decontamination with chlorine dioxide, the BSC must be sealed at the
exhaust opening and front opening to eliminate exposure to chlorine
dioxide, of the power supplies, PCBs and other components under the
front canopy as they are susceptible to damage from the process.

s

Thermo Fisher Scientific has developed validated procedures for the
decontamination of the Thermo Scientific 1300 Series A2 using the Steris
VHP (Vaporized Hydrogen Peroxide) system. These procedures are
available on request.

Change the UV

Disinfection Time

1300 Series A2 5-5Thermo Scientific

Section 5

Cleaning and Decontamination

Clean the exterior surfaces of the cabinet using a solution of tepid water
and commercially available mild dishwashing agent. Then, dry all surfaces
well, using a soft, clean cloth.

For cleaning, the window can be lowered beyond the closing position (Use
of the Window, Section 1).

Creating a gap at the window’s upper edge ensures that the upper portion
of the window can be cleaned and/or disinfected. Use a commercially
available window cleaner to clean the window.

Clean the drain pan, using a solution of tepid water and commercially
available mild dishwashing agent.

1. Remove the work tray(s) from the chamber.

2. Clean thoroughly to remove any residues and/or deposits.

3. Wipe the drain pan, using a clean cloth and plenty of clean water.

4. Discard any liquid in the drain pan. Rinse and dry thoroughly.

Note After cleaning, make sure that all cleaning product has been removed
completely from the drain pan.

s

5. Re-install the work tray.

Clean Exterior

Surfaces

Clean Window

Clean the Drain Pan

The protective paper catch grids are
retained in the airduct wall by their
own tension.

1. To remove a grid section, push
the retaining tab down until the
section can be moved out from
under the inner back wall.

2. To install the grid section, first
place it on the lip of the back
wall, then push the retaining tab
down and towards the back until
the locking tabs are secured
behind the inner back wall.

Caution Do not operate the unit
without the paper catch grids
installed!

s

5-6 1300 Series A2 Thermo Scientific

Section 5

Cleaning and Decontamination

Figure 5-1. Protective Paper Catch Grid

Clean the Paper

Catch Grid

Retaining
tab

Grid
section

Grid section

Inner
back wall

Retaining
tab

Section 6 Maintenance

The SmartFlow Performance Indicator displays the status of the
compensation reserve.

Green indicators show the fan and filters have power and capacity in
reserve to respond to additional demands. Yellow or red indicators show
that while the cabinet is providing personnel and product protection,
maintenance should be scheduled to restore the compensation reserve.

Regardless of the display on the SmartFlow Indicator, the biological safety
cabinet should be certified annually.

The annual certification consists of the following checks:

• Electrical safety in accordance with national regulations.

• Functional test of the device.

• Checking all components for possible damage.

• Checking the filter state.

• Verifying device air flows

• Checking the airflow conditions.

• Repeat test in accordance with NSF/ANSI 49.

Warning The diffuser plate on the chamber ceiling serves as protection for
the downflow filter and prevents refluxing of downflow air. When
scanning the filter surface for a leak test, the perforated plate must be
removed.

s

1300 Series A2 6-1Thermo Scientific

As filter replacement is an interference with the safety system of the unit,
filters must only be replaced by adequately trained and authorized service
personnel.

Warning For any service work in a potentially contaminated section of the
biological safety cabinet, the unit must first be decontaminated.

s

The sample chamber is illuminated by one (4 ft units) or two (3, 5 & 6 ft
units) fluorescent bulbs. The fluorescent bulbs are installed in the light
mounting frame in the sample chamber (Fig. 6-1).

1. Switch the unit off and disconnect it from the power source.

2. Move the window to the cleaning position (below closed position) to
ensure a sufficient gap between the window’s upper edge and the light
dome.

3. The fluorescent bulbs are installed in rotating sockets. To remove,
rotate the bulb counterclockwise to disengage the latch and remove it
from the sockets.

4. To install, slide the bulb contact pins into the rotating socket grooves
and rotate the bulb clockwise to latch the sockets.

The optional, factory installed UV light is installed in the chamber ceiling
immediately behind the window. The UV light should be replaced after
1500 operating hours. See Figure 6-1.

1. Turn the unit off and disconnect it from the power source.

2. Move the window to the maximum open position.

3. Wear protective gloves to prevent skin oils from burning into the bulb.

The UV bulb is installed in rotating sockets. To remove, rotate the
bulb counterclockwise to disengage the latch and remove it from the
sockets.

4. To install, slide the bulb contact pins into the rotating socket grooves
and rotate the bulb clockwise to latch the sockets.

6-2 1300 Series A2 Thermo Scientific

Section 6

Maintenance

Service

Sample Chamber Lights

Optional UV Lights

1300 Series A2 6-3Thermo Scientific

Section 6

Maintenance

Caution Remote alarm systems can be retrofitted and integrated into the
unit controls. Alarm contacts change state based on window position.
Airflow changes from window position could impair personnel and
product protection. Installation of these systems should only be performed
by authorized service personnel.

s

The entire cabinet with the exception of the HEPA filters can be discarded
after having been thoroughly cleaned and decontaminated. The HEPA
filters must be discarded in accordance with the applicable national, state
and local regulations for special solid waste.

Warning As this unit can be used for processing and treating infectious
substances, it may become contaminated. Prior to disposal, the entire unit
with filters must be decontaminated in accordance with acceptable
standards and procedures.

s

Replacements and

Repairs

UV light

Figure 6-1. Light Replacement

Unit Disposal

Chamber light

Section 7 Specifications

1300 Series A2 7-1Thermo Scientific

Dimensions

Description

4ft Models (10” window opening work position)
1345, 1346, 1354, 1355, 1356, 1375, 1376, 1384,
1385, 1386

4ft Models (8” window opening work position)

1305, 1306, 1325, 1326, 1335, 1336, 1395, 1396

6ft Models (10” window opening work position)
1347, 1348, 1357, 1358, 1359, 1377, 1378, 1387,
1388, 1389

6ft Models (8” window opening work position)

1307, 1308, 1327, 1328, 1337, 1338, 1397, 1398

Exterior dimensions

Width mm / in 1300 / 51.2 1900 / 74.8

Depth mm / in 800 / 31.5

Height mm / in 1568 / 61.8

Interior dimensions

Width mm / in 1200 / 47.2 1800 / 70.9

Depth mm / in 630 / 24.8

Height mm / in 780 / 30.7

Front window

Working position mm / in

254 / 10 - pertains to all 4’ & 6‘ models listed

above with 10” window work position

203 / 8 - pertains to all 4’ & 6’ models listed above
with 8” window work position

Opening max. mm / in 535 / 21.1

Working area height with adjustable height stand

Height Range mm / in 750 / 29.5 to 950 / 37.4

SmartPort - Diameter mm / in 76.0 / 3.0

Remaining side wall

access ports -Diameter

mm / in 23 / 0.91

SmartPort - Distance

lower edge

mm / in 365.8 / 14.4

Remaining upper ports -

Distance edge

mm / in 226.1 / 8.9

Remaining lower ports -

Distance lower edge

mm / in 353.1 / 13.9

Distance from back panel

SmartPort mm / in 182.9 / 7.2

First access port mm / in 259.0 / 10.2

Second access port mm / in 309.9 / 12.2

Third access port mm / in 360.7 / 14.2

7-2 Model 1300 Series A2 Thermo Scientific

Section 7

Specifications

Dimensions

Description

3ft Models (10” window opening work position) -

1320, 1321, 1322, 1323, 1330, 1331, 1332, 1333

3ft Models (8” window opening work position) -

1339, 1340, 1341, 1342, 1390, 1391, 1392, 1393

5ft Models (10” window opening work position) -

1368, 1369, 1370, 1371, 1380, 1381, 1382, 1383

5ft Models (8” window opening work position) -

1350, 1351, 1352, 1353, 1366, 1367, 1372, 1373

Exterior dimensions

Width mm / in 1000 / 39.4 1600 / 63.0

Depth mm / in 800 / 31.5

Height mm / in 1568 / 61.8

Interior dimensions

Width mm / in 900 / 35.4 1500 / 59.1

Depth mm / in 630 / 24.8

Height mm / in 780 / 30.7

Front window

Working position mm / in

254 / 10 - pertains to all 3’ & 5‘ models listed

above with 10” window work position

203 / 8 - pertains to all 3’ & 5’ models listed above

with 8” window work position

Opening max. mm / in 535 / 21.1

Working area height with adjustable height stand

Height range mm / in 750 / 29.5 to 950 / 37.4

SmartPort - Diameter mm / in 76.0 / 3.0

Remaining side wall

access ports -Diameter

mm / in 23 / 0.91

SmartPort - Distance

lower edge

mm / in 365.8 / 14.4

Remaining upper ports -

Distance edge

mm / in 226.1 / 8.9

Remaining lower ports -

Distance lower edge

mm / in 353.1 / 13.9

Declaration of Conformity available from the factory, on request.

1300 Series A2 7-3Thermo Scientific

Section 7

Specifications

Ambient conditions (indoor use only)

Temperature

Max. ambient temp during operation °C / °F 40 / 104

Min. ambient temp during operation °C / °F 10 / 50

Humidity

Max. humidity / operation % r.H. 90, non-condensing

Max. humidity / storage % r.H. 95

Heat dissipation to environment

Room temperature 20°C kJ/s / BTU/s

4ft models 10": 0.2 / 0.19

4ft. models 8": 0.18 / 0.17

6ft. models 10": 0.4 / 0.38
6ft. models 8": 0.36 / 0.34

Room temperature rise

Above room temp with window closed °K < 2 < 2

Ergonomics

Noise level* dB(A)

4ft. models 10”: 63, ±2

4ft. models 8": 62, ±2

6ft models 10”: 65, ±2

6ft. models 8": 64, ±2

*The noise level was determined in accordance with NSF/ANSI 49. The measurement uncertainty is within a range of ± 2 dB.

Volume, weights, and loads

Description

4ft Models (10” window opening work
position) 1345, 1346, 1354, 1355, 1356,
1375, 1376, 1384, 1385, 1386

4ft Models (8” window opening work
position) 1305, 1306, 1325, 1326, 1335,
1336, 1395, 1396

6ft Models (10” window opening work
position) 1347, 1348, 1357, 1358, 1359,
1377, 1378, 1387, 1388, 1389

6ft Models (8” window opening work
position) 1307, 1308, 1327, 1328, 1337,
1338, 1397, 1398

Volume

Drain pan l / gal 20 / 5.3 30 / 7.9

Weights

Cabinet kg / lb 200 / 441 280 / 617

Stand kg / lb 26.3 / 58.0 30.0 / 66.1

Loads

Maximum load on work surface kg / lb 25 / 55

Maximum load on overall working area kg / lb 50 / 110 75 / 165

7-4 1300 Series A2 Thermo Scientific

Section 7

Specifications

Ambient conditions (indoor use only)

Temperature

Max. ambient temp during operation °C / °F 40 / 104

Min. ambient temp during operation °C / °F 10 / 50

Humidity

Max. humidity / operation % r.H. 90, non-condensing

Max. humidity / storage % r.H. 95

Heat dissipation to environment

Room temperature 20°C kJ/s / BTU/s

3ft models 10": 0.17 / 0.16

3ft. models 8": 0.15 / 0.14

5ft. models 10": 0.31 / 0.29

5ft. models 8": 0.28 / 0.27

Room temperature rise

Above room temp with window closed °K < 2 < 2

Ergonomics

Noise level* dB(A)

3ft. models 10”: 63, ±2

3ft. models 8": 62, ±2

5ft models 10”: 65, ±2

5ft. models 8": 64, ±2

*The noise level was determined in accordance with NSF/ANSI 49. The measurement uncertainty is within a range of ± 2 dB.

Volume, weights, and loads

Description

3ft Models (10” window opening work
position) - 1320, 1321, 1322, 1323, 1330,
1331, 1332, 1333

3ft Models (8” window opening work
position) - 1339, 1340, 1341, 1342, 1390,
1391, 1392, 1393

5ft Models (10” window opening work
position) - 1368, 1369, 1370, 1371, 1380,
1381, 1382, 1383

5ft Models (8” window opening work
position) - 1350, 1351, 1352, 1353, 1366,
1367, 1372, 1373

Volume

Drain pan l / gal 15 / 4.0 25 / 6.6

Weights

Cabinet kg / lb 170 / 375 230 / 507

Stand kg / lb 24.4 / 53.8 28.1 / 62.0

Loads

Max. load on single work surface module kg / lb 25 / 55

Maximum load on overall working area kg / lb 50 / 110 75 / 165

1300 Series A2 7-5Thermo Scientific

Section 7

Specifications

Electrical data

Models
(Nominal voltages ±10%)

120V Models - 4 ft / 6 ft

1305, 1307, 1325, 1327, 1335, 1337, 1345,

1355, 1375, 1385 / 1347, 1357, 1377, 1387,

1395, 1397

230V Models - 4 ft / 6 ft

1306, 1308, 1326, 1328, 1336, 1338, 1346,

1354, 1356, 1376, 1384, 1386 / 1348, 1358,

1359, 1378, 1388, 1389, 1396, 1398

120V Models - 3 ft / 5 ft

1320, 1321, 1322, 1323, 1339, 1340, 1341,

1342, 1350, 1351, 1352, 1353, 1368, 1369,

1370, 1371

230V Models - 3 ft / 5 ft

1330, 1331, 1332, 1333, 1366, 1367, 1372,
1373, 1380, 1381, 1382, 1383, 1390, 1391,

1392, 1393

Voltage

Rated voltage V 1/N/PE AC, 120V, 50/60 Hz 1/N/PE AC, 230V, 50/60 Hz

Blower voltage V 48 V DC

Current

Power consumption maximum A 8 / 10 7 / 8

Leakage current IEC 61010-1,

UL61010-1

mA < 3.5

Motherboard fusing A 2 x T 15 A

Outlet fusing A 2 x T 5 A

On-site fusing A Circuit breaker 15 A / Fuse T 15 A

Power

Power input maximum * W 1130 / 1700 1440 / 2000

Protection

Protection class I

Protection type IP 20

Overvoltage category (IEC 61010-1,

UL61010-1)

II

Contamination degree (IEC 61010-1,

UL61010-1)

2

Connecting lines

Mains connection

Power Cord (>3 m / 10 ft)

with NEMA 5-15 plug

CEE 7/7 Plug

* With additional 5A load on cabinet receptacles, blowers at 100% and cabinet lighting switched on.

7-6 1300 Series A2 Thermo Scientific

Section 7

Specifications

Airflow system

Description

4ft Models (10” / 8” window opening work
position) 1305, 1306, 1325, 1326, 1335,
1336, 1345, 1346, 1354, 1355, 1356, 1375,
1376, 1384, 1385, 1386, 1395, 1396

6ft Models (10” / 8” window opening work
position) 1307, 1308, 1327, 1328, 1337,
1338,1347, 1348, 1357, 1358, 1359, 1377,
1378, 1387, 1388, 1389, 1397, 1398

Airflows / Airflow velocities

Inflow m/s 0.53

ft/min 105

Downflow m/s 0.32

Downflow ft/min 63

Air volume

Overall volume flow

m³/h

(10”/8”)

1415 / 1298 2122 / 1947

ft³/min

(10”/8”)

833 / 764 1249 / 1146

Downflow volume flow

m³/h

(10”/8”)

829 1244

ft³/min

(10”/8”)

488 732

Exhaust air volume flow

m³/h

(10”/8”)

585 / 468 878 / 702

ft³/min

(10”/8”)

344 / 276 517 / 413

Filters

Type HEPA

Material Fiberglass

Separability in MPPS % 99.995

Separability at 0,3 μm particle size % 99.999

Downflow

Width mm / in 1220 / 48 1830 / 72

Depth mm / in 457 / 18

Height mm / in 94 / 3.7

Exhaust air

Width mm / in 610 / 24 915 / 36

Depth mm / in 457 / 18 457 / 18

Height mm / in 117 / 4.6 117 / 4.6

1300 Series A2 7-7Thermo Scientific

Section 7

Specifications

Airflow system

Description

3ft Models (10” / 8” window opening work
position) - 1320, 1321, 1322, 1323, 1330,
1331, 1332, 1333, 1339, 1340, 1341, 1342,
1390, 1391, 1392, 1393

5ft Models (10” / 8” window opening work
position) - 1350, 1351, 1352, 1353, 1366,
1367, 1368, 1369, 1370, 1371, 1372, 1373,
1380, 1381, 1382, 1383

Airflows / Airflow velocities

Inflow m/s 0.53

ft/min 105

Downflow m/s 0.32

Downflow ft/min 63

Air volume

Overall volume flow

m³/h

(10”/8”)

1061 / 973 1768 / 1622

ft³/min

(10”/8”)

625 / 573 1041 / 955

Downflow volume flow

m³/h

(10”/8”)

622 1037

ft³/min

(10”/8”)

366 610

Exhaust air volume flow

m³/h

(10”/8”)

439 / 351 732 / 585

ft³/min

(10”/8”)

258 / 207 431 / 344

Filters

Type HEPA

Material Fiberglass

Separability in MPPS % 99.995

Separability at 0,3 μm particle size % 99.999

Downflow

Width mm / in 915 / 36 1525 / 60

Depth mm / in 457 / 18

Height mm / in 94 / 3.7

Exhaust air

Width mm / in 610 / 24

Depth mm / in 457 / 18

Height mm / in 117 / 4.6

1300 Series A2 8-1Thermo Scientific

Section 8 Certification Testing

For application in the USA, the unit has been rated as a Class II safety
cabinet, Type A2, in accordance with NSF/ANSI 49.

For operation as a unit of said classifications, a certification test and repeat
test in accordance with NSF/ANSI 49, Annex F should be completed at
the time of installation, and at least annually thereafter. As described in
Annex F, recertification should be performed whenever HEPA filters are
changed, maintenance repairs are made to internal parts, or the cabinet is
relocated. More frequent recertification should be considered when
working with safety-critical materials (three to six months).

Nominal value:

Default value as specified by Thermo.

Measured value:

Value measured at the location of the safety cabinet.

Tolerance:

Acceptable deviation from the nominal value.

Average value:

The sum of the measuring values divided by the number of tests. The
average value is compared to the nominal value.

Setpoint:

Acceptable operating value for the inflow and downflow velocities.

Inflow velocity (FPM):

Velocity of the air entering the sample chamber opening.

Downflow velocity (FPM):

Velocity of the displacement flow circulating through the work chamber.

Exhaust velocity (FPM):

Velocity of the airflow discharged through the exhaust filter opening.

Exhaust airflow volume (CFM):

Amount of air discharged at the exhaust filter.

Classification of the

Safety Cabinet

Test Terms

According to NSF/ANSI 49, tests related to the safety of the personnel,
product and environment that must be conducted on-site for Class II,
Type A2 biological safety cabinets, at a minimum frequency of annually,
are;

• downflow velocity profile test

• inflow velocity test

• airflow smoke patterns test

• HEPA filter leak test

• site installation assessment tests

– alarm functions

– exhaust system performance (proper exhaust duct negative pressure

and canopy performance).

As described in Annex F, in addition to the above, the following tests
performed at the request of the customer, or at the discretion of the
certification provider.

• comfort and safety tests

– lighting intensity

– noise level

– vibration

– electrical leakage, ground circuit resistance and polarity tests

Note Service with costs: Unless certification was expressly called for in the
specification, quotes and/or purchase order, the cost for this on-site testing
is to be paid for by the customer.

s

8-2 1300 Series A2 Thermo Scientific

Section 8

Certification Testing

Testing

For conducting these tests, Thermo recommends testing equipment of the
manufacturers listed below.

Inflow Velocity – DIM Method (Primary)

Description: Inflow velocity using the direct inflow measurement
method (DIM).

Equipment: . . .Shortridge ADM-870, or equivalent

. . . . . . . . .Flow hood Series 8400, or equivalent

Method:

1. Move the front window to the work position (25.4cm/10 in. or

20.3cm/8 in., depending on model).

2. Attach the flow hood [1] in the work opening [2] and seal the
remaining open areas.

3. Operate the system blowers for approx 20 min.

4. Record at least 5 measurements of the inflow air volume.

5. Average those readings and calculate the inflow velocity (V1) as
described below.

1300 Series A2 8-3Thermo Scientific

Section 8

Certification Testing

Test Equipment

Testing equipment Manufacturer Application

Smoke tubes

MSA
Pittsburgh, Pennsylvania, 15230

Airflow pattern test

Thermoanemometer

TSI
Shoreview, Minnesota 55126

Downflow velocity test and alternate method for inflow

velocity using constricted access

Digital Safety Inspector

Ohmic Instruments Co.
Easton, Maryland 21601

Electrical leakage and ground resistance tests

GFI Circuit Tester

Leviton Manufacturing
Little Neck, New York, 11362

Electrical leakage, ground resistance, and polarity tests

Photometer

Air Techniques
Owing Mills, Maryland, 21117

Filter leak test

Aerosol Generator

Air Techniques
Owing Mills, Maryland, 21117

Generation of aerosol mist

Vibration Meter

Quest Technologies
Oconomowoc, Wisconsin, 53066

Vibration test

Flow Hood Air Data Multimeter

Shortridge Instruments. Inc.
Scottsdale, Arizona, 85260

Inflow velocity test

Testing Information

Inflow Area:

• 3ft Models (10” window opening work position) 1320, 1321, 1322,
1323, 1330, 1331, 1332, 1333: Front opening 2.46 sq ft

3ft Models (8” window opening work position) 1339, 1340, 1341,
1342, 1390, 1391, 1392, 1393: Inflow area for 8” models: 1.97 sq ft

• 5ft Models (10” window opening work position) 1368, 1369, 1370,
1371, 1380, 1381, 1382, 1383: Front opening 4.10 sq ft

5ft Models (8” window opening work position) 1350, 1351, 1352,
1353, 1366, 1367, 1372, 1373: Inflow area for 8” models: 3.28 sq ft

• 4ft Models (10” window opening work position) 1345, 1346, 1354,
1355, 1356, 1375, 1376, 1384, 1385, 1386: Front opening 3.28 sq ft

4ft Models (8” window opening work position) 1305, 1306, 1325,
1326, 1335, 1336, 1395, 1396: Inflow area for 8” models: 2.62 sq ft

• 6ft Models (10” window opening work position) 1347, 1348, 1357,
1358, 1359, 1377, 1378, 1387, 1388, 1389: Front opening 4.92 sq ft

6ft Models (8” window opening work position) 1307, 1308, 1327,
1328, 1337, 1338, 1397, 1398: Inflow are for 8” models: 3.93 sq ft

Calculation:

• Inflow Velocity (V1) = Inflow Air Volume (V2)/Actual Inflow Area (A2)

Acceptance:

• 100 – 110 FPM

Correction measures:

• Enter the service level and activate S2, change S2 until the desired veloci­ty is reached and save the new value.

• If the velocity can't be adjusted due to clogging of the filters, change the
exhaust filter.

• If the filter is in order, change the exhaust blower.

Note Check of alarm limits: If S1 or S2 are changed, the alarm limits S3
and S4 must be checked and saved again.

s

8-4 1300 Series A2 Thermo Scientific

Section 8

Certification Testing

Testing Information

(continued)

Inflow Velocity – Constricted Window Method (Secondary)

Description:

• Inflow velocity measured at several points through a constricted window.

Equipment:

• Thermal Anemometer, or equivalent (hotwire)

• Anemometer Probe Holder, part number 1911325

Method:

1. Lower the front window to a
height of 3 in. (7.6cm).

2. Operate the system blowers for
approximately 20 minutes.

3. Insert the thermal anemometer
probe into the probe holder.
Place the probe holder at the
window opening work surface,
ensuring it is seated properly.
See Figure 8-1. Adjust the
probe so the air passing through
probe is centered in the window
opening (1.5 in. [3.8cm]).

Note The thermoanemometer
probe must be positioned at a 10°
angle from front vertical and
located on the same plane as the
inside of the window glass.

4. Beginning 5.9 inches in from
the left inside wall, record
successive readings every 5.9
inches (5 readings for 3 ft, 7
readings for 4 ft, 9 readings for
5 ft, and 11 readings for 6 ft
models).

5. Average those readings and
correct result for true velocity.

6. Calculate the inflow velocity as
described below.

1300 Series A2 8-5Thermo Scientific

Section 8

Certification Testing

Testing Information

(continued)

Figure 8-1. Constricted Window Method

5.9”

5.9”

3”

1.5”

3”

Calculation:

• Inflow velocity (V1) = average of actual inflow velocity * K factor * 0.3
for 10” window opening work position, 0.375 for 8” window opening
work position

K factor = 1.0219 for 3 ft w/ 10” opening, 0.9935 for 3 ft w/ 8” opening.

K factor = 0.9918 for 4 ft w/ 10” opening, 1.047 for 4 ft w/ 8” opening.

K factor = 0.9942 for 5 ft w/ 10” opening, 1.0012 for 5 ft w/ 8” opening.

K factor = 0.9918 for 6 ft w/ 10” opening, 1.100 for 6 ft w/ 8” opening.

Acceptance:

• 100 – 110 FPM

Refer to the data plate on the lower right corner of the unit face.

s

Inflow Velocity – Calculating Exhaust Flow (Alternative)

Description:

• Exhaust velocity measurements to determine inflow velocity.

Equipment:

• Thermal Anemometer, or equivalent (hotwire)

• Freestanding Fixture, or equivalent

Method:

1. Operate the unit in the work position for approximately 20 minutes.

2. Insert the anemometer probe into the probe stand, adjusting the height
to 4 inches (10cm) above the exhaust filter.

3. Record the readings on a horizontal plane 4 inches (10cm) above the
effective exhaust filter area, in a grid pattern not to exceed 4 inches
(10cm) x 4 inches (10cm).

4. Average the readings and calculate the inflow velocity.

Grid Pattern:

•

3, 4 & 5 ft Models 1305, 1306, 1320, 1321, 1322, 1323, 1325, 1326, 1330,
1331, 1332, 1333, 1335, 1336, 1339, 1340, 1341, 1342, 1345, 1346, 1350,
1351, 1352, 1353, 1354, 1355, 1356, 1366, 1367, 1369, 1370, 1371, 1372,
1373, 1375, 1376, 1380, 1381, 1382, 1383, 1384, 1385, 1386, 1390, 1391,
1392, 1393, 1395, 1396

20 points

8-6 1300 Series A2 Thermo Scientific

Section 8

Certification Testing

Testing Information

(continued)

3.94

2.98

2.98

2.98

3.94

3.94 3.74 3.74 3.74 3.74 3.94 inches

• 6ft Models 1307, 1308, 1327, 1328, 1337, 1338,1347, 1348, 1357, 1358,
1359, 1377, 1378, 1387, 1388, 1389, 1397, 1398

32 points

Effective Exhaust Filter Area:

• 3, 4 & 5 ft Models 1305, 1306, 1320, 1321, 1322, 1323, 1325, 1326, 1330,
1331, 1332, 1333, 1335, 1336, 1339, 1340, 1341, 1342, 1345, 1346, 1350,
1351, 1352, 1353, 1354, 1355, 1356, 1366, 1367, 1369, 1370, 1371, 1372,
1373, 1375, 1376, 1380, 1381, 1382, 1383, 1384, 1385, 1386, 1390, 1391,
1392, 1393, 1395, 1396: 16.8 x 22.8”, 2.66 sq ft

• 6ft Models 1307, 1308, 1327, 1328, 1337, 1338,1347, 1348, 1357, 1358,
1359, 1377, 1378, 1387, 1388, 1389, 1397, 1398: 16.8” x 34.8”, 4.06 sq ft

Inflow Area:

• 3ft Models (10” window opening work position) 1320, 1321, 1322, 1323,
1330, 1331, 1332, 1333: Front opening 2.46 sq ft

3ft Models (8” window opening work position) 1339, 1340, 1341, 1342,
1390, 1391, 1392, 1393: Inflow area for 8” models: 1.97 sq ft

• 5ft Models (10” window opening work position) 1368, 1369, 1370, 1371,
1380, 1381, 1382, 1383: Front opening 4.10 sq ft

5ft Models (8” window opening work position) 1350, 1351, 1352, 1353,
1366, 1367, 1372, 1373: Inflow area for 8” models: 3.28 sq ft

• 4ft Models (10” window opening work position) 1345, 1346, 1354, 1355,
1356, 1375, 1376, 1384, 1385, 1386: Front opening 3.28 sq ft

4ft Models (8” window opening work position) 1305, 1306, 1325, 1326,
1335, 1336, 1395, 1396: Inflow area for 8” models: 2.62 sq ft

• 6ft Models (10” window opening work position) 1347, 1348, 1357, 1358,
1359, 1377, 1378, 1387, 1388, 1389: Front opening 4.92 sq ft

6ft Models (8” window opening work position) 1307, 1308, 1327, 1328,
1337, 1338, 1397, 1398: Inflow are for 8” models: 3.93 sq ft

Calculation:

• Average exhaust velocity (V2) = Sum of measurements / Number of
readings

• Exhaust Volume (V3) = Average velocity (V2) x Effective filter area (A3)

• Inflow velocity (V1) = Exhaust volume (V3) / Work opening area (A2)

Acceptance:

• 100 – 110 FPM

1300 Series A2 8-7Thermo Scientific

Section 8

Certification Testing

3.94

2.98

2.98

2.98

3.94

3.94 3.85 3.85 3.85 3.85 3.85 3.85 3.85 3.94 inches

Testing Information

(continued)

Downflow Velocity – Uniform Cabinet

Description:

• Measures the velocity of air flow onto the work surface through the sup­ply filter.

Equipment:

• Thermal Anemometer, or equivalent (hotwire)

• Freestanding Fixture, or equivalent

Method:

1. Operate the unit in the work position for approximately 20 minutes.

2. Insert the anemometer probe into the probe stand, adjusting for a
height of 4 inches (10 cm) above the bottom edge of the window
opening while in the work position.

3. Record the readings on a horizontal plane 4 inches (10 cm) above the
bottom edge of the window opening while in the work position, in a
grid pattern not to exceed 6 inches (15 cm) x 6 inches (15 cm).

Grid Pattern:

• 3 ft Models - 1320, 1321, 1322, 1323, 1330, 1331, 1332, 1333, 1339,
1340, 1341, 1342, 1390, 1391, 1392, 1393

• 4 ft Models 1307, 1308, 1327, 1328, 1337, 1338,1347, 1348, 1357,
1358, 1359, 1377, 1378, 1387, 1388, 1389, 1397, 1398

8-8 1300 Series A2 Thermo Scientific

Section 8

Certification Testing

Testing Information

(continued)

6

5.5

5.5

6

6 3.9 3.9 3.9 3.9 3.9 3.9 6 inches

5.7 for
8 in
opening
models

21 points

6

5.5

5.5

6

6 5.88 5.88 5.88 5.88 5.88 5.88 6 inches

5.7 for
8 in
opening
models

21 points

Grid Pattern (continued):

• 5 ft Models - 1350, 1351, 1352, 1353, 1366, 1367, 1368, 1369, 1370,
1371, 1372, 1373, 1380, 1381, 1382, 1383

• 6 ft Models 1307, 1308, 1327, 1328, 1337, 1338,1347, 1348, 1357,
1358, 1359, 1377, 1378, 1387, 1388, 1389, 1397, 1398

Calculation:

• Average Downflow Velocity = Sum of all measurements / Number of
readings

Acceptance:

• 58-68 FPM

Correction measures:

• Enter the service level and activate S1, change S1 until the desired veloc­ity is reached and save the new value.

• If the velocity can’t be adjusted due to clogging of the filters, change the
supply filter.

• If the filter is in order, change the supply blower.

Note Check of alarm limits: If S1 or S2 are changed, the alarm limits S3
and S4 have to be checked and saved again.

s

1300 Series A2 8-9Thermo Scientific

Section 8

Certification Testing

Testing Information

(continued)

6

5.5

5.5

6

6 5.88 5.88 5.88 5.88 5.88 5.88 5.88 5.88 5.88 5.88 6 inches

5.7 for
8 in
opening
models

33 points

6

5.5

5.5

6

6 5.87 5.87 5.87 5.87 5.87 5.87 5.87 5.87 6 inches

5.7 for
8 in
opening
models

27 points

8-10 Model 1300 Series A2 Thermo Scientific

Section 8

Certification Testing

Description:

• Determines the integrity of the downflow and exhaust filters.

Equipment:

• Aerosol Photometer, or equivalent

• Aerosol Generator, or equivalent

Method:

1. Allow the cabinet to operate for approximately 20 minutes.

2. Remove the work tray and protective covers, as appropriate.

3. Introduce the aerosol from the generator in the center rear of the work
area, using a tee fitting to evenly distribute the aerosol.

4. If desired, and if the cabinet has not been used with hazardous
materials since an effective decontamination, the upstream
concentration can be measured. If desired, connect the photometer
sample hose to the appropriate (supply or exhaust) test hose connection
(exhaust test hose - right side / supply test hose - left side) from under
the work tray.

5. Using either the appropriate upstream challenge measurement or
calculated challenge value, set the photometer accordingly.

6. Scan the downstream side and perimeter of the filter as prescribed in
NSF/ANSI 49, Annex F.

Acceptance:

• Filters scanned – 0.01% of upstream concentration at any point

HEPA Filter Leak Test

Filters that can be

Accessed and Scanned

1300 Series A2 8-11Thermo Scientific

Section 8

Certification Testing

Method:

1. Set up as in Steps 1 through 5 above

2. In the duct, downstream of the filter, sample in accordance with
NSF/ANSI 49.

Acceptance:

• Filters not scanned – 0.005% of upstream concentration

Description:

• Determines the internal and external behavior of the air movement.

Equipment:

• Cold smoke (titanium tetrachloride)

Method:

• Downflow:

Pass smoke across the centerline of the work surface, 4 inches (10 cm)
above the work opening.

• View Screen Retention:

Pass smoke 1 inch (2.5 cm) behind the window opening, 6 inches (15
cm) above the work opening.

• Work Opening Edge Retention:

Pass smoke around the entire outside work opening, approximately 1.5
inches (3.8 cm) away from the cabinet.

• Window Seal Test:

Pass smoke up both sides and across the top of the window opening
from inside the work area, approximately 2 inches (5 cm) from the
edges.

Acceptance:

• No smoke shall have dead spots, reflux or escape the cabinet once drawn
in.

Airflow Pattern Test

Filters that cannot be

Accessed or Scanned

Description:

• Refer to UL 61010-1

Description:

• Verify the unit is integrated properly into the facility.

Description:

• Verify the airflow alarms will activate within 15 seconds when there is a
20% loss.

Equipment:

• Thermal Anemometer or equivalent (hotwire)

• Anemometer probe holder, part 1911325

• Shortridge ADM-870 or equivalent

• Flowhood series 8400 or equivalent

Method:

• Downflow:

Enter the service level and activate S03.

Verify that the average downflow velocity is 20% lower than the rated
velocity by following the method described previously. Change and save
S03 if needed and leave the service level. Enter the service level again
and set S01 to the value of S03 -0.5%. The LED "AIRFLOW STA­BLE" must change from green to red. Leave the service level without
saving this value by pressing the "acknowledge alarm" key.

•Inflow:

Enter the service level and activate S04.

Verify that the inflow velocity is 20% lower than the rated velocity by
following one method described previously. Change and save S04 if
needed and leave the service level.

Enter the service level again and set S02 to the value of S04 -0.5%.

The LED "AIRFLOW STABLE" must change from green to red. Leave
the service level without saving this value by pressing the "acknowledge
alarm" key.

Airflow Alarms

8-12 1300 Series A2 Thermo Scientific

Section 8

Certification Testing

Elect. Leakage , Ground

Resistance, Polarity Tests

Site Installation

Assessment Tests

1300 Series A2 8-13Thermo Scientific

Section 8

Certification Testing

Airflow Alarms (continued)

Acceptance:

• Unit alarm activates within 15 seconds when the downflow and inflow
velocity is set to the correct values and the LED „Airflow STABLE“
changes from green to red (as described above).

Description:

• Verify the window alarms are operating properly

Equipment:

• Operating manual

Method:

• Raise the window 1 inch (2.5 cm) above work position

Acceptance:

• Window alarm activates

Description:

• Verify the exhaust system is functioning properly

Canopy Connections

Equipment:

• Cold smoke (titanium tetrachloride)

Method:

• Pass smoke around the air gap, to ensure negative pressure exists

Acceptance:

• No smoke refluxes back into the room once drawn into the canopy.

Sash Alarms

Exhaust System Performance

1300 Series A2 9-1Thermo Scientific

Device type: Part number:

Serial number: Service number:

Location: Operator's note:

Technician / Company Notes Date Signature

THERMO FISHER SCIENTIFIC LAMINAR FLOW EQUIPMENT WARRANTY USA

The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows shipping time
so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection
extends to any subsequent owner.

During the first thirty-six (36) months, component parts proven to be non-conforming in material or workmanship will be
repaired or replaced at Thermo’s expense, including labor. Installation, calibration and certification is not covered by this
warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior
to performance of any repairs. Expendable items, glass, filters and gasket are covered by this warranty to the extent that
Thermo Fisher Scientific determines that they were defective at the time of delivery to the carrier.

Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give
prior approval for return of any component or equipment. At Thermo’s option, all non-conforming parts must be returned to
Thermo postage paid and replacement parts are shipped FOB destination.

THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages to lost profits or
loss of products.

Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.

If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or
1-740-373-4763. We’re ready to answer your questions on equipment warranty, operation, maintenance, service, and
special applications. Outside the USA, contract your local distributor for warranty information.

Rev. 0 4/12

ISO

9001

REGISTERED

THERMO FISHER SCIENTIFIC LAMINAR FLOW EQUIPMENT WARRANTY INTERNATIONAL

The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows shipping
time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protec­tion extends to any subsequent owner.

During the first thirty six (36) months, component parts proven to be non-conforming in material or workmanship will be
repaired or replaced at Thermo’s expense, excepting labor. Installation, calibration and certification is not covered by this
warranty agreement. The Technical Services Department must be contacted for warranty determination and direction
prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.

Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give
prior approval for return of any component or equipment. At Thermo’s option, all non-conforming parts must be returned
to Thermo postage paid and replacement parts are shipped FOB destination.

THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages to lost profits
or loss of products.

Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.

If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA or Canada) or
1-740-373-4763. We’re ready to answer your questions on equipment warranty, operation, maintenance, service, and
special applications. Outside the USA, contract your local distributor for warranty information.

Rev. 5 4/09

ISO

9001

REGISTERED

Thermo Fisher Scientific
401 Millcreek Road
Marietta, Ohio 45750
United States

www.thermofisher.com