kruss P3000 User Manual
Polarimeter
P3000
User Manual
innovation since 1796
www.kruess.com
Table of Contents
Chapter - 1 Introduction ................................................................................... 5
1.1 Application Examples ..................................................................... 5
1.2 Reading Information ....................................................................... 6
Chapter - 2 General Safety Instructions ............................................................ 7
Chapter - 3 Instrument Description ................................................................... 9
3.1 Scope of Delivery ............................................................................ 9
3.2 Optional Accessories ...................................................................... 9
3.3 Required Reagents ......................................................................... 9
Calibration .................................................................................. 9
Accuracy Check ........................................................................... 9
Sample Dilution ........................................................................... 9
3.4 Instrument Images ........................................................................ 10
Instrument Front View of P3000 .................................................. 10
Instrument Back View of P3000 ................................................... 10
Polarimeter Tubes ...................................................................... 10
3.5 Screen Descriptions ...................................................................... 11
Main Menu ............................................................................... 11
Explanation of Buttons ................................................................ 11
Explanation of Display Window ................................................... 12
3.6 Measurement Principle .................................................................. 13
Chapter - 4 Basic Operation ............................................................................. 14
4.1 Buttons ......................................................................................... 14
4.2 Defining the System Settings ........................................................16
4.3 Defining the Instrument Settings ................................................... 17
4.4 Performing the Calibrations .......................................................... 18
Chapter - 5 Initial Startup ................................................................................ 19
5.1 Installation .................................................................................... 19
Choosing a Suitable Location ..................................................... 19
Connecting a Printer .................................................................. 19
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Connecting the Polarimeter to the Power Supply ........................... 19
5.2 Preparing the Instrument after Installation ................................... 20
Switching on the Instrument ........................................................ 20
Switching on the Printer .............................................................. 20
Connecting the Temperature Sensor ............................................ 21
Calibrating the Temperature Sensor ............................................ 21
Checking (Validating) the Instrument ............................................ 22
Chapter - 6 Routine Operation ........................................................................ 26
6.1 Preparing the Instrument .............................................................. 26
Switching on the Instrument ........................................................ 26
Changing the Instrument Parameters ........................................... 27
6.2 Executing a Measurement ............................................................. 29
Determining the Blank ................................................................ 29
Resetting the Blank ..................................................................... 30
Supplying a Sample ................................................................... 30
Connecting a Temperature Sensor .............................................. 30
Starting the Measurement ........................................................... 31
Measurement ............................................................................ 31
After Measurement ..................................................................... 31
6.3 Measurement Results ................................................................... 32
Measurement Result Display ....................................................... 32
Printing the Displayed Measurement Result ................................... 32
Switching off the Instrument ........................................................ 32
Chapter - 7 Settings ....................................................................................... 33
7.1 System Settings ........................................................................... 33
Opening the Menu .................................................................... 33
Explanations .............................................................................. 34
Switching the Printer on and off ................................................... 35
Selecting the Language .............................................................. 36
Input of Date and Time .............................................................. 37
7.2 Instrument Settings ...................................................................... 38
Opening the Menu .................................................................... 38
Explanations .............................................................................. 39
Selecting the Scale ..................................................................... 40
Selecting the Weighted Sugar Sample .......................................... 40
Selecting the Polarimeter Tube .................................................... 42
Selecting the Temperature .......................................................... 43
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7.3 Calibrating the Measurement Range ............................................. 44
Opening the Menu .................................................................... 44
Canceling the Calibration ........................................................... 45
Resetting the Calibration ............................................................. 46
Performing the Calibration .......................................................... 46
Blank Measurement ................................................................... 46
Input of the Quartz Standard ....................................................... 46
Input of the Quartz Temperature ................................................. 48
Measuring the Quartz Standard .................................................. 49
Accepting the Calibration ........................................................... 49
Rejecting the Calibration ............................................................ 50
7.4 Calibrating the Temperature Sensor .............................................. 51
Prerequisites .............................................................................. 51
Calling the Function ................................................................... 51
Measuring the Temperature ........................................................ 52
Input of the Thermometer Temperature ........................................ 53
7.5 Blank ............................................................................................ 56
Calling the Blank Function .......................................................... 56
Resetting the Blank ..................................................................... 57
Measuring the Blank .................................................................. 57
Saving the New Blank ................................................................. 58
Rejecting the New Blank ............................................................. 58
Chapter - 8 Maintenance, Care & Inspection ................................................... 59
8.1 Regular Maintenance and Care ..................................................... 59
Daily ......................................................................................... 59
Weekly ...................................................................................... 59
Yearly ....................................................................................... 59
When necessary ......................................................................... 59
8.2 Changing the Fuse ........................................................................ 60
8.3 Maintenance by Service ................................................................ 60
Chapter - 9 Troubleshooting Table ................................................................. 61
Chapter - 10 Transport, Storage and Disposal .................................................. 62
Transport .................................................................................. 62
Storage ..................................................................................... 62
Disposal .................................................................................... 62
Chapter - 11 Appendix ...................................................................................... 63
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11.1 Technical Specifications ............................................................... 63
11.2 Ordering Information .................................................................... 65
11.3 Consumables ................................................................................ 66
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Chapter - 1 Introduction / 1.1 Application Examples
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Chapter - 1 Introduction
The Polarimeter P3000 is very easy to operate and is designed for the fully automated
measurement of optical rotation.
The measurement sample is filled into the polarimeter tube, inserted into the sample
chamber and measured. The results are shown on the display and, if connected, printed
out on a printer.
The operator dialog is available in German and English.
1.1 Application Examples
Application areas for the polarimeter are, amongst
others:
Pharmaceutical Industry
Monitoring of chemical processes
Purity control and determination of
concentrations
Testing of substances listed in the German
Pharmacopoeia (DAB)
Chemical Industry
Purity control and determination of
concentrations
Analysis of optically active components
(qualitative und quantitative)
Determination of configuration changes
Monitoring of chemical processes
Food Industry
Determination of concentrations
Purity control
Quality control
Sugar and Sweetener-Industry
Quality control of raw materials and end
products
Determination of fructose and glucose
Cosmetic Industry
Beverage Industry / Breweries
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Chapter - 1 Introduction / 1.2 Reading Information
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1.2 Reading Information
Although instrument operation of the P3000 is easy, we recommend attentive reading of
this manual to ensure correct and optimal use of all functions.
Symbols This manual uses the following symbols:
Listings
Instructions to do something
Text in italics refers to text in dialog windows.
Reference to further information in this manual.
Finding Information
Look up in the table of contents.
Should a problem occur, refer to Chapter - 9
Troubleshooting Table
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Chapter - 2 General Safety Instructions
Chapter - 2 General Safety Instructions
Hazards during normal specified operation:
Danger!
Electric shock by damaged instrument or power cable.
Before switching on the instrument, make sure that
the instrument and the power cable are
undamaged.
Only operate instruments that have been properly
installed and maintained.
Danger!
Electric shock by entering liquids.
Before cleaning, switch off the instrument and
disconnect the power supply.
Do not allow liquids to enter the instrument.
Do not spray the instrument for cleaning.
Make sure the instrument is completely dry before
connecting to the power supply.
Danger!
Electric shock
Before changing the fuse, switch off the instrument
and disconnect the power cable.
Warning!
Damage to the retina by the LED light source.
Do not look directly into the LED light source.
Careful!
Danger of pinching your hand.
Close the sample chamber lid carefully.
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Chapter - 2 General Safety Instructions
Careful!
Careful!
Attention!
Damage to electrical components by condensate
formation.
Wait at least one hour after transporting the
instrument in a cooler environment (e.g. cold room
or outdoors) before connecting to the power
supply.
Overheating damage.
Do not place the instrument near a heat source
(e.g. heating).
Do not expose to direct sunlight.
Allow for unhindered air circulation.
Aggressive chemicals may damage the instrument's
paint or the plastic housing!
Clean the instrument with neutral cleaning agents
only.
DO NOT use aggressive chemicals for cleaning,
such as strong or weak acids or bases, acetone,
formaldehyde, chlorinated hydrocarbons or
phenols.
If the instrument comes into contact with aggressive
chemicals, clean immediately with a neutral
cleaning agent.
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Chapter - 3 Instrument Description / 3.1 Scope of Delivery
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Chapter - 3 Instrument Description
This chapter describes the following topics:
Scope of delivery
Optional accessories
Instrument images
Required reagents
Screen description
Measurement principle
3.1 Scope of Delivery
The following items are included in the delivery of the polarimeters P3000:
Polarimeter P3000
Power cable
Touchscreen pen
Polarimeter tubes:
1 x 100 mm glass tube with middle funnel
1 x 200 mm glass tube with middle funnel
User manual
3.2 Optional Accessories
Printer
various polarimeter tubes
3.3 Required Reagents
Calibration For calibration of the instrument, quartz standards are
used.
See Ordering Information.
Accuracy Check For checking the accuracy of the instrument's
measurements you may use:
Quartz standards
Standard solutions with known concentrations
Sample Dilution The solution used for sample dilution depends on the
sample material to be measured and should not itself
be optically active.
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Chapter - 3 Instrument Description / 3.4 Instrument Images
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3.4 Instrument Images
Instrument Front View of
P3000
Instrument Back View of
The P3000 provides the following connections:
P3000
Sample chamber port
Touchscreen user interface
Printer connection (for direct result output)
Power supply connection with power switch and
fuse
Power switch & fuse &
power supply connection
Printer connection
(serial interface)
Polarimeter Tubes The polarimeter tube is available in two different
lengths:
100 mm
200 mm
various other types optionally available
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Chapter - 3 Instrument Description / 3.5 Screen Descriptions
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3.5 Screen Descriptions
This chapter briefly describes the following screens of the polarimeter P3000:
Main Menu In the main menu the measurements are started and
the results are displayed. The buttons lead to further
submenus.
Display window for results, unit, blank as well as
instrument and system settings.
Operation of buttons via touchscreen.
Explanation of Buttons
Opens the menus for :
System settings
Instrument settings
Calibration (measurement range,
temperature sensor)
Blank
Provides the following information:
Instrument and firmware settings
Date of last calibration
Version of user interface and data
base
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Chapter - 3 Instrument Description / 3.5 Screen Descriptions
11.000 °Z
sensor is connected to the
Prints the result shown on the display.
Starts the measurement.
Explanation of Display
Window
Measurement result of the sample with
unit.
Blank Blank used for calculation of the sample
result.
Connected printer is switched on.
The motor is switched on.
No display: motor is switched off. It
switches off automatically a few seconds
after the measurement.
The temperature
sample polarimeter tube and the
measured temperature during sample
measurement is 20.0 °C in this example.
Explanations: see Chapter 7.4 Calibrating the
Temperature Sensor.
No temperature display: no temperature
sensor connected.
Size of the employed polarimeter tube.
Is selected in the instrument settings.
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Selected wavelength (fixed setting).
Chapter - 3 Instrument Description / 3.6 Measurement Principle
3.6 Measurement Principle
The polarimeter measures the optical rotation of the orientation of plane-polarized light
caused by optically active substances. In principle, it consists of two polarization filters,
the polarizer and the analyzer, each of which allows only a certain orientation of planepolarized light to pass through.
The polarimeter measures the optical activity or the optical rotation of a chemical
substance.
The following equation defines the relation between the optical rotation, the specific
rotation and the sample concentration:
c = concentration [g/l]
α = measured optical rotation [°]
l = tube length [dm]
T
[α]
= specific rotation, dependent on temperature (°C) and wavelength
The optical rotation is, amongst others, dependent on the following parameters:
• Type of sample
• Concentration of the optically active components
• Light wavelength
• Temperature of sample
• Tube length
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Chapter - 4 Basic Operation / 4.1 Buttons
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Chapter - 4 Basic Operation
The instrument is operated by a touchscreen display using four touchscreen buttons.
4.1 Buttons
Operation of the polarimeter is performed using these
four buttons:
Opens the submenus for :
System settings (printer, language, date/time)
Instrument settings (scale, polarimeter tube,
temperature)
Calibration (measurement range, temperature
sensor)
Blank
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Returns to the main menu.
Selection of desired settings (system settings,
instrument settings, instrument calibration,
blank).
Opens the menu, e.g. for system settings.
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Chapter - 4 Basic Operation / 4.1 Buttons
Opens the wiindow with the following information
about the P3000:
Instrument and firmware settings.
Date of last calibration.
Version of the user interface and data base.
Prints the result shown in the display.
Starts the measurement.
Returns to the main menu.
Page up and page down in the
information pages.
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Chapter - 4 Basic Operation / 4.2 Defining the System Settings
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4.2 Defining the System Settings
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Switching between the parameters:
Printer,
Language,
Day, Month, Year, Hour, Min, Sec.
Selection of the desired setting:
on/off (printer)
German/English (language)
Date/time:
Counts up the respective selected
parameter (Day, Month, Year, Hour,
Min, Sec).
Accepts the set values and returns to the
main menu.
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Chapter - 4 Basic Operation / 4.3 Defining the Instrument Settings
4.3 Defining the Instrument Settings
Selection of parameters (scale, polarimeter
tube, temperature).
Selection of parameter setting.
Accepts the set values and returns to the
main menu.
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