Microbiological Safety Cabinet class II EN 12469 certified by TÜV
USER MANUAL
Serial number:
Date of delivery:
Customer:
Kojair Tech Oy Tel. +358 3 471 7000 info@kojair.com
Teollisuustie 3 Fax +358 3 471 7077 www.kojair.com
35700 Vilppula
FINLAND
The definition of the Microbiological Safety Cabinet is as follows:
A ventilated enclosure intended to offer protection to the user and environment from the aerosols
arising from the handling of potentially hazardous and hazardous micro-organisms, with discharge to
the atmosphere being filtered.
You are owner of one of the most modern and silent Class II Microbiological Safety Cabinets
available. The Golden Line –series is microprocessor-controlled and therewith widely customizable.
All the standard-configurations of Golden Line series are type-tested and approved by TÜV
Rheinland according to current EN –standard 12469:2000 (the standard for Biosafety cabinets)
NOTICE! Only use this product for its intended purpose.
rev.3.01
1. FEATURES AND SAFETY ........................................................................................ 4
1.1. Introduction to the cabinet .................................................................................. 4
1.2. Construction materials ....................................................................................... 5
1.3. Positioning the cabinet and safety instructions .................................................. 5
2. USING THE CABINET ............................................................................................... 7
2.1. Control panel ...................................................................................................... 7
2.2. What t o do at first start-up .................................................................................. 9
2.3. Control panel functions .................................................................................... 12
2.3.1 User settings ..................................................................................................... 13
2.4. Daily maintenance and cleaning ...................................................................... 14
2.5. UV-light ............................................................................................................ 16
2.5.1. instruction for UV-light ...................................................................................... 17
2.5.1.1. introduction ...................................................................................................... 17
2.5.1.2 UV-light button .................................................................................................. 17
2.5.1.3 timer in the menu .............................................................................................. 18
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rev.3.01
NOTE! Only Class III Microbiological Safety Cabinets can be used for working
1. FEATURES AND SAFETY
1.1. INTRODUCTION TO THE CABINET
Microbiological safety cabinets are intended to reduce the risk to the operator when handling hazardous
or potentially hazardous micro-organisms. They do not necessarily protect the operator from all the
hazards involved. Some types of cabinet can also protect the materials being handled in them from the
environmental contamination.
Kojair Golden Line is a Class II Microbiological Safety Cabinet operating on the recirculation principle. It
protects the products handled on the work surface from contamination and safeguards the operator
against substances hazardous to health.
Picture 1. Kojair Golden Line Class II Safety Cabinet
Examples of the uses of the cabinet are cellular culture, handling pathogenic micro-organisms, isotopes,
sterile work for operations, handling medical substances in hospitals and pharmacies as well as the
production and maintenance of electronic items in which particle-free air is a necessity.
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with Group 4 pathogens. The Kojair BW3 is a class III safety cabinet which
protects the user and the environment from Group 4 pathogens.
Please contact your local Kojair representative for more information.
rev.3.01
NOTE! The Cabinet may crash. Use special attention when moving and
transportation equipment.
The basic principle of the cabinet is that the replacement air moves through the grid at the front edge of
the work surface to the bottom basin and then again to the recirculation system. Simultaneously it forms
an air curtain in the work opening, which prevents air from flowing outwards.
The recirculated air inside the cabinet is purified as it flows through the HEPA-filter and it flows laminarly
through the workspace. (See picture 2).
Picture 2. Principle of the airflow
1.2. CONSTRUCTION MATERIALS
The main construction material of the cabinet is painted mild steel. The work surface and the collecting
pan located below it are made of stainless steel (AISI 304).
The front and side walls are made of 6 mm thick laminated safety glass.
1.3. POSITIONING THE CABINET AND SAFETY INSTRUCTIONS
installing the cabinet. Take into account the center of gravity when placing
For more information about the cabinets safe handling and moving see the service manual part
2.2
The cabinet should be positioned so that there are no external or conflicting airflow disturbances when it
is operating such as open windows or personnel passing in front of it.
The cabinet should be switched on at least 15 minutes before starting the work so there is time to clean
the workspace. Before starting the actual work it is recommended that the work is carefully prepared so
that equipment and material are moved in and out of the cabinet as little as possible.
A continuous in-and-out movement disturbs the airflow and weakens the protection given by the cabinet.
For the cabinet to give the best possible protection, you should try to minimize rapid hand and arm
movements as much as possible.
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rev.3.01
However, it is better if the cabinet can always be in the normal operating
NOTE! Before starting work, check that HEPA-filter on the roof of the
workspace is intact.
NOTE! Beware that you do not obstruct the airflow by obstructing the grid on
the worktable. The safe working area is between the grids (see picture 3.).
Picture 3. Safe working area.
While you are working, the front glass of the cabinet should be in the working position. If the front glass
is not in the correct position, the user will hear immediate warning from the alarm. If you intend to switch
off the cabinet when you have finished working, it is better to close the front window before shutting
down the cabinet.
mode. When you are not working in the cabinet, close the front glass and
device automatically switches to half speed mode. See chapter, 2.1 Control
panel.
It is strongly recommended that gas flames are used on the left side of the work space as short time as
possible. That is because the flame can disturb the airflow and it will warm up the recirculated air, which
can cause an alarm.
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rev.3.01
Red led is used for indicating alarms. When the fan has been
during power failure.
If the exhaust air speed is not correct the general alarm –LED and
If the recirculated air speed is not correct the general alarm –LED
The front glass of the cabinet should be in the working position
Press this button to start the cabinet. (Press for few seconds to
start)
4.
Lights
You can switch on workspace lights on using this button.
2. USING THE CABINET
2.1. CONTROL PANEL
CONTOL PANEL
CONTOL BUTTON / ALARM LED FUNCTIONAL DESCRIPTION
switched on the general alarm -LED lights up. The general alarm LED lights stays on until the air speed is sufficient. If sufficient air
speed is not reached in 5 minutes, the cabinet gives an acoustic
alarm.
1. Alarm indicators
1. A Exhaust air alarm
1. B Recirculated air alarm
1. C The front window wrong position
2. Alarm resetting button Not in use in this cabinet model
Working in the cabinet is not allowed while the visual and/or
acoustic alarm is activated. If al arm go es on st op wo rk ing a nd
contact the person in charge of the laboratory.
If the power failure occurs the general alarm LED –lights up and the
acoustic alarm will sound. Working in the cabinet is not allowed
the exhaust air alarm –LED will light up and the acoustic alarm will
sound.
and the recirculated air alarm –LED will light up and the acoustic
alarm will sound.
during the work. If the front window is not in the correct position
both the general alarm –LED and the front window alarm –LED will
light up and the acoustic alarm will sound.
3. Start button
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rev.3.01
With this button you can lift up the front window. When you lift up
higher excluding while cleaning or service.
9.
+
With this button you can browse through menus and set values
Red led is used for indicating alarms. When the fan has been
contact the person in charge of the laboratory.
You can switch cabinet to half speed mode using this button. The
The UV-light socket is in the workspace back wall; if you wish to use
Used with optional gas valve. If you use liquid gas during the
The gas valve works only when the cabinet is at 1/1 speed mode.
16.
-
With this button you can browse through menus and set values
5. Socket This button activates the socket.
6. Front window up
7. Lcd –display
8. Mode With this button you can go to the menu.
10. General alarm
11. Start fan or switch fan to ½ speed
12. Uv –light (option)
the front glass from its low est posit ion , it sto ps auto mat ic to
operating position. It is not allowed to lift the front glass any
switched on the general alarm -LED lights up. The general alarm LED lights stays on until the air speed is sufficient. If sufficient air
speed is not reached in 5 minutes, the cabinet gives an acoustic
alarm.
Working in the cabinet is not allowed while the visual and/or
acoustic alarm is activated. If alarm goes on, sto p working and
workspace lighting will shut down and the cabinet will give an
acoustic and visual alarm. The acoustic alarm signal lasts for only a
few seconds.
the UV-light, push the UV-light button on the control panel.
There are two modes for UV-light, button or timer.
For more information about using UV-light, see part 2.5.
If button is selected, the UV-light will stay on until UV-button is
pressed again. If timer is selected, the cabinet asks for shutdown
time, which is selectable between 20 ... 120 min with 10 min
interval. Time can be changed with + and - buttons and time is
selected with SET button. UV-light is switched off after selected
time.
13 Magnetic valve
14. Front window down You can move down the front window using this button
15. Set With this button you can confirm selection.
procedure, first you have to push the magnetic valve button from
the control panel. This enables the gas to flow.
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rev.3.01
NOTE! Before using the cabinet, perform the installation tests (listed in
NOTE! Despite the automatic anti-collisio n sy st e m, while dr i v ing stand up or
2.2. WHAT TO DO AT FIRST START-UP
chapter –Testing in service manual) and clean the cabinet carefully. See the
cleaning instructions in section 2.4 Daily maintenance and cleaning
Check if the cabinet’s power cable is in the socket outlet (230 VAC, 50 Hz) and the other lead is in the
electric plug on the top of the cabinet. Check also that other lead of the electrical base stands own
power cable is in the socket outlet (230 VAC, 50 Hz).
Adjust the correct table top height with the electrical base stand. Adjustment can be done with control
panel of the base stand. Control panel is located in the front edge of the electrical base stand (see
picture 4.)
Picture 4. Control panel of the electrical base stand.
Using the control panel:
For safety reasons control panel has a locking function.
For safety reasons electrical base stand has been equipped with an anti-collision system. If the base
stand hits an obstacle while driving stand up or down, base stand drives automatically stand 4cm in the
opposite direction.
If the panel is locked, only a bar is lightning in the centre of the display. To unlock the control press “S”
for 1 sec, When the control is unlocked, the height will be shown in the display. Now the panel is active
for 2,5 seconds. When pressing on of the keys you can do your adjustment. Hereafter the panel will be
active for 5 seconds. When there are no activations made within the mentioned time slot, the panel will
go in to locking mode again. Also if you press “S” for unlocking too long, the panel will unlock but
immediately lock again.
The panel is divided into parallel and memory drive. The two arrow buttons are used for parallel drive
and the four buttons for memory drive (see picture 5.)
down, do not keep your feet under the stand, and make sure that there are no
obstructions on top of the cabinet and under the electrical base stand.
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rev.3.01
Picture 5, Control buttons of the control panel
Normal operation:
To run the base stand up and down, press the “desk up” and desk down” arrow button, keep it pressed
until the base stand reaches the desired height. The display will count the height as the base stand is
moving and will continually show the height of the desk after stopping.
Storing a memory position:
Touch the “S” button the display will flash “S” for 3 sec. While the “S” is displayed, touch the memory
button 1, 2 or 3. The display will acknowledge by showing S1, S2 or S3 for 1sec. To abort a store
sequence press the “desk up” or desk down” arrow button while the “S” is flashing, or wait the 3 seconds
until the display automatically returns to show the height of the base stand.
Drive to a stored position:
Press memory button 1, 2 or 3 and the system will start driving to the desired memory position. Keep the
button activated until the position is reached. The display will count the height as it is driving to the
memory position.
Customizing the digital display to show actual table top height:
The panel will show 68cm as the height of the base stand as the default setting. To adjust the dis play
touch the “S” button and the “desk up” or “desk down” button at the same time until the display shows
the actual height of the table top.
Changing between cm and inches:
Touch the “S” button for approximately 4 seconds and the unit of measurement will change on the
display. The default setting depends on the type chosen.
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rev.3.01
Turn the mains on by the key-switch (located on the right side of the front panel, see picture 6.). The
self-test can take 1 to 5 minutes and during the self-test the cabinet does not take any orders. During
self-test the red general alarm -LED lights up, other led’s blink and cabinet gives an acoustic alarm
(acoustic alarm lasts only a few seconds). Do not touch the controls during the self-test.
Picture 6. Key switch connects cabinets main power on or off.
The fan and the lights can be switched on by the buttons located in the control panel. Before switching
the fan on you should check that the outlet on the roof of the cabinet is not blocked so that the filtered
exhaust air can be freely exhausted out of the cabinet.
TO START UP CABINET PUSH BUTTON 3. (Press for few seconds to start)
If sufficient air speed is not reached in 5 minutes, the cabinet gives an acoustic alarm.
The key lock is activated by pressing SET -button and START -button in sequence. When the key lock
is activated the panel can be safely wiped, etc. without risking unwanted commands. Green led’s in the
front window up and front window down -buttons are blinking to indicate lock mode. The setting can be
deactivated by pressing the SET -button and START -button in sequence.
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rev.3.01
2.3. CONTROL PANEL FUNCTIONS
The diagram below represents the hierarchy on menu options available in Kojair Golden Line cabinet.
Press MODE button to enter menu. At first there is a selection user/service with - / + buttons. Service
level requires password. Only Kojair Tech Oy authorized service technician is allowed to access service
level.
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rev.3.01
For more information about using UV-light, see part 2.5.
timer settings.
Set the alarm limits to the fan and UV-light. The limit can be selected between
5000h.
2.3.1 USER SETTINGS
Functions under the user settings are for “daily use” and using these functions
Doesn’t harm protection level of the cabinet, that ´s why you do not have to set any password to get
access to them. All the text in LCD-display is shown with capital letters and texts can be shown with six
different languages (see table below )
Start the cabinet by the main key switch. The LCD-display shows the current software version of
controls and the cabinet also begins a self-test.
MODE – opens the menu and you can browse it by using + and - buttons
SET – allows you to select a function from the menu and / or confirm selection.
Press button 8. MODE
Display shows: + =SERVICE - =USER
Select USER level with [-]
By pressing – and + buttons the user can scroll through the functions. A function is selected by pressing
button SET button.
User setting functions are described in the table below
USER SETTING
FUNCTION DESCRIPTION
Regulates the intensity in the working area between 60 % and 100 %. By pressing the
SET LIGHT LEVEL
SET UV TIMER
SET SOCKET TIMER
SHOW UV LIGHT AGE
[+] or [-] -buttons the display shows the value in 1% intervals. To confirm the desired
value press SET –button. The light intensity changes when the SET –button is
pressed.
Select first the function set or browse.
In set mode you can select daily or weekly mode. In daily mode you select start and
end times with [+/-] buttons. The function is selected by pressing SET. In weekly
mode you select first the day with [+/-] buttons, accept it with SET button and select
finally the start and end times like in daily mode. Exit with MODE button. If you want
to delete timer settings, see below.
In read mode you can check or reset the timer settings. The display shows first the
weekly settings, with next step down [-] you can move in settings, mode button reset
the timing. The display shows “Deleted” after every reseted timing and when all
timings have been reseted, the display shows “Empty”. After that you can make the
same with daily settings. With SET –button you can go back if do not want to delete
The display shows how long time the UV-light has been on.
RESET UV COUNTER Resets the hour counter for the UV-light.
FAN RUNNING TIME This function shows the time the fan has been running.
SET LIMIT VALUES
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2000 - 10000h for fan and 1000 – 10000 for UV-light. Factory setting for UV-light is
rev.3.01
NOTE! Handle the front window with extra care. See picture 7.
NOTE! Before maintenance and cleaning it is mandatory always to check
be issued by the person in charge of the laboratory.
FAN 1/1 SPEED
FAN 1/ 2 SPEED
SET TIME
SET LANGUAGES Selects the language: English, Swedish, German, Finnish, French, Dutch
Shows the rotation speed of the fan to normal operation speed or ½ speed. The
current speed, which is between 0-100 % for 1/1 speed and 0 – 50% for ½ speed, is
seen on the display.
NOTE! The values can’t be changed.
Set the clock to real-time. Change the value with +/- buttons, with SET button to the
next field/ok.
HH:MM YYYY MO/DD WD
HH=hours MM=minutes YYYY=year
MO=month DD=day WD=day of the week
2.4. DAILY MAINTENANCE AND CLEANING
To ensure the safety when cleaning the front glass, confirm the operation together with at least
one person.
Turn the key switch on. The switch is located on the right bottom edge of the front panel.
Drive the front window to highest position. In order to clean the back of the front window you have to lift
it up carefully. Do not lift the glass from it ends because the glass may bend and crack.
See the correct lifting points from the picture 7.
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Picture 7.
what kind of substances have been handled in the cabinet. If cytostatics or
other hazardous substances have been handled in the cabinet special care
has to be taken. The person who is cleaning and servicing the cabinet should
use a mask, protective gloves and an overall. More detailed instructions will
rev.3.01
NOTE! The use of dissolvents for cleaning is not permitted.
NOTE! Do not clean front glass with detergents or disinfectants around
glass plate (see the picture 8)
Picture 8. Do not clean front glass with detergents or disinfectants around glass plate. Area is ma rked
with red in the picture.
Clean the workspace of the cabinet with mild detergents and disinfectants with a 70 % alcohol solution.
You can wipe off the alcohol that has been sprayed on or you can simply let it evaporate.
The cabinet’s outer surfaces should be cleaned by wiping with neutral or mild base detergent solutions.
Clean the work surface inside the cabinet. The lower surface and collecting pan should always be wiped
during cleaning (Picture 9).
Picture 9.
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rev.3.01
NOTE! UV-light is not recommended for decontamination by the EN 12469.
NOTE! When cleaning the workspace of the cabinet you should be careful
NOTE! The operating time of UV-light’s tube is about 5000 hours. Within that
about the filter located above the workspace and also the sensor of the down
flow indicator (picture 10.) in the back wall of the workspace. They can be
easily damaged when wiping the cabinet and therefore should not be touched.
Picture 10. Down flow indicator
2.5. UV-LIGHT
You can also use the UV-light (option) for decontaminating the workspace; it takes 30 to 60 minutes.
You can use the timer for timing the decontamination. See instruction below.
UV-light must not be switched on when working in the same room. UV-radiation can harm eyes
and skin of operators and cause damages to other materials.
Picture 11. UV-light is switched on.
time interval of the radiation power decreases to 85%. For max. efficiency it is
recommended the UV-tube to be changed after 5000 hours.
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rev.3.01
2.5.1. INSTRUCTION FOR UV-LIGHT
This instruction serves Kojair SL- GL- and XL-cabinets with UV-light selection.
2.5.1.1. INTRODUCTION
UV-light fixture itself is a separate unit which is to be placed on the back wall of the cabinet’s working
chamber with magnet fastenings and plugged in the UV-outlet at the back wall of the working chamber
next to the 230VAC outlet (Picture 12.).
The UV-light can be operated from user interface by pushing the UV-light butto n (Picture 13.) or by
selecting timer and programming function in use.
Picture 12.
Picture 13.
2.5.1.2 UV-LIGHT BUTTON
When pushing the UV-light button the display will show two alternatives:
[+] = Button [-] = Timer
If [+] (immediate usage) is chosen the display asks to close the front window.
UV-light will then lit after closing the front window and stays on until the UV-light button is pushed again
or if the front window is opened.
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rev.3.01
NOTE ! When using timer function, the UV-light is turned on at the
NOTE! All selected weekly and daily times are active. If only one
deleted.
If you choose [-], the display will show time selection. You can select disinfection time with [+] or [-]
buttons to 20-120 min with 10 min intervals. Confirm selection by pushing “SET”. The display then asks
to close the front window. UV-light will lit after closing the front window and stays on until the set time
has elapsed, UV-light button is pushed again or if the front window is opened.
2.5.1.3 TIMER IN THE MENU
Go to the timer functions by pushing "MODE" and select user level by pushing [-].
Go to “Set UV timer” with [+] or [-] buttons and press “SET”.
The display will show now [+] = Browse and [-] = Set.
By pushing [+] (Browse) all weekly and daily settings can be browsed through and also deleted one by
one by pushing “MODE” = delete.
By pushing [-] (Set) the display will ask to select [+] = Weekly or [-] = Daily.
If you choose “Daily”, there will be in the display ON 00:00 and OFF 00:00.
The On-time means the exact clock time when the UV-light will turn on and it turns off at the Off-time.
Select hours and minutes by pushing [+] or [-] buttons and confirm each selection by pushing “SET”.
If you choose “Weekly”, you can choose exact days of the week, when you want to use the UV-light
timing.
Choose a day with buttons [+] or [-] and press “SET” to confirm.
Set the time, when you want the UV-light go on and off. Confirm by pressing “SET”
You can repeat this as many times you need.
When you are done, press “MODE”.
Use [+] or [-] buttons to find “Exit” and press “MODE”.
Close the window.
Example of timer’s daily setting:
UV-light is wanted to be turned on every day of the week at 22:00 – 23:00
-Push “MODE”
-Push “-“
-Push “-“ until there is “Set UV timer” in the display
-Push “SET”
-Push “-“
-Push “-“
-Push “-“ until the hours are at 22
-Push “SET” (to confirm hours”
-Push “SET” (to confirm minutes)
-Push “-“ until the hours are at 23
-Push “SET” (to confirm hours)
-Push “SET” (to confirm minutes)
-Push “+” until there is “Exit” in the display
-Push “MODE”
-Push “window down”-button until the window is closed
selected times only if the front window is closed totally.
certain selection is supposed to be used, all others must be
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rev.3.01
NOTE! In general for daily maintenance the cabinet requires only
inspections.
cleaning. Technicians trained by Kojair Tech Oy will determine
needs for other maintenance, including filter change, in yearly
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