Knauer Azura P 6.1L User Manual

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Pump P 6.1L User Manual

V6890

Note: For your own safety, read the manual and always observe the warnings and

safety information on the device and in the manual

Manuel en français: Si jamais vous préfériez un manuel en français pour ce produit, veuillez vous

contacter le support technique (Technical Support) par email ou par fax avec le no. de série. Merci beaucoup.

Technical Support: Phone: +49 30 809727-111 (9-17h, Central European Time)

Fax: +49 30 8015010 E-Mail: support@knauer.net Languages: German, English

Publisher: KNAUER Wissenschaftliche Geräte GmbH

Hegauer Weg 38 D-14163 Berlin Phone: +49 30 809727-0 Fax: +49 30 8015010 Internet: www.knauer.net E-Mail: info@knauer.net

Version information: Article number: V6890

Version number: 2.2 Last update: 2017-02-16 Original edition

The information in this document is subject to change without prior notice.

For latest version of the manual, check our website:

http://www.knauer.net/en/downloads.html

KNAUER Wissenschaftliche Geräte GmbH.

3Table of Contents

Product Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Operating Ranges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Device Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Views . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Rear View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Performance Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Safety for Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Unpacking the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Capillaries and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Connecting the Solvent Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Pre-Installed Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Integrating the Pump into an HPLC Flow System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Isocratic pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Binary pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Connecting the Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Connecting the Solvent Selection Valve to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . 18

Quaternary Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Connecting the Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Connecting the 4-Channel Degasser to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Connecting the Valve Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Connecting the Piston Backflushing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Connecting the Leak Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Computer Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Setting a Static IP Address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

P 6.1L user manual V6890

Table of Contents 4

Pin Header Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Remote Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Events Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Connecting Cables to the Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Analog Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Initial Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Bleeding the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Switch-On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Operating with Chromatography Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Operating with Mobile Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Meaning of the LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Functional Tests IQ and OQ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Possible Problems and Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Maintenance and Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Maintenance Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Checking the Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Flushing the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Putting the Pump Out of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Disconnecting the Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Preparing the Pump Head for Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Dismounting the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Removing the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Cleaning the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Installing the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Inline Filter on the Pressure Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Removing the Inline Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Inserting the New Inline Filter Cartridge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Replacing the Mixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Removing a Leak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

P 6.1L General Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Main Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

P 6.1L user manual V6890

5Table of Contents

Device Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

P 6.1L isocratic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

P 6.1L binary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

P 6.1L quaternary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Pump Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Repeat Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Accessories and Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Legal Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

Warranty Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

HPLC Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

P 6.1L user manual V6890

Product Information

Note: Only use the device for applications that fall within the range of the intended use. Otherwise, the protective and safety equipment of the device could fail.

Description The pump P 6.1L is suitable for analytical and semi-preparative applications.

Operating Ranges

Operating ranges The device can be used in the following areas:

 biochemical analysis  chemical analysis  food analysis  pharmaceutical analysis  environmental analysis

Device Variants

The P 6.1L is available in three different options:

 Isocratic pump  Binary pump (HDG pump)  Quaternary Pump (NDG pump)

All variants are available as a standard version made of stainless steel or as a bio-inert version made of ceramics with PEEK capillary, PEEK connectors, and PEEK venting screw.

Product Information 6

Isocratic pump The isocratic pump is equipped with a pressure sensor with an integrated

Legend

1 Pressure sensor

2 Pump head

Views

Front View

inline filter and a venting screw..

Fig. 1 Isocratic pump - front view

P 6.1L user manual V6890

7 Product Information

345

12 3 4 5

Binary pump The binary pump consists of two pump drives and a 2-channel degasser

with a Solvent Selection valve. A pressure sensor with an integrated inline filter and a venting screw are also part of the pump.

Legend

1 Pressure sensor

2 Mixer

3 Pump head

4 Degasser

5 Solvent Selection

valve

Fig. 2 Binary pump - front view

Quarternary pump The quaternary pump consists of a pump, a valve block and a 4-channelde-

gasser. A pressure sensor with an integrated inline filter, a venting screw,and a mixer are also part of the pump.

Legend

1 Pressure sensor

2 mixing chamber

3 Pump head

4 Valve block

5 Degasser

Fig. 3 Binary pump - front view

P 6.1L user manual V6890

Service Interface Please note that the service interface is solely used for repair and mainte-

Legend

1 serial number

2 service interface

3 Pin header

4 LAN connector

5 Power connection

and power switch

Product Information 8

Rear View

On the back of pump, the connectors for LAN and power chord are locatedas well as the serial number of the device an the power switch (on/off).

nance tasks performed by service technicians.

Fig. 4 Rear view (example)

Performance Features

The pump is used to convey liquids, either in an HPLC system or as a standalone device. Liquids are conveyed either with constant flow or with constant pressure. The flow and pressure, which liquids can be conveyed with, depend on the pump head.

Pump heads Pump heads for several areas of application are available for the pump P

6.1L:

Standard Bio-inert

Size 5 ml, 10 ml and 50 ml 10 ml and 50 ml

material Pump head with stainless

steel inlays

Operating Range

Every pump head is equipped with an RFID chip. It is used to monitor and save all important parameters and settings. The RFID technology offers the following advantages:

 Software automatically recognizes the parameters of the pump head  All service-relevant data of the pump head is stored

 HPLC  Dosing applications

with non-aggressive media

Pump head with ceramic inlays

 Purification/FPLC  Ion chromatography

Mixer Volume of the mixer is 100 l. The mixer is exchangeable and can be

replaced by mixers with a volume of 50 µl or 200 µl . The parameters necessary for the mixer are set via Mobile Control or via

chromatography software.

P 6.1L user manual V6890

9 Product Information

Parameter stainless steel Bio-Inert

Size 50 µl, 100 µl, 200 µl 250 µl

Pressure 1000 bar 400 bar

Piston backflushing The piston backflushing function automatically flushes the rear piston area

of the pump head upon switch-on and in continuous mode.

 Upon switch-on: The rear piston area of the pump head is automatically

flushed for 15 seconds.

 In continuous mode: The rear piston area of the pump head is flushed

automatically every 15 minutes, for 15 seconds.

PMax Mode This mode protects the column from excessive fluctuations in pressure. If

the maximum pressure is reached, the user can decide if the pump should be switched off or continue running with constant pressure. You find a detailed description on chromatography software in the corresponding user manual.

GLP Data The Mobile Control and the different software products (ClarityChrom

OpenLAB

) can be used to display or read GLP data. You find a detailed description on how to display or read out GLP data in the respective user manuals.

Optional accessories The following optional accessories are available:

 Pump head cooler for the pump head  Mobile Control

Eluents

Even small quantities of other substances, such as additives, modifiers, or salts can influence the durability of the materials.

If there is any doubt, contact the Technical Support of the manufacturer.

Unsuitable Eluents

 halogenated hydrocarbons, e.g. Freon®  concentrated mineral and organic acids  concentrated bases  eluents containing particles  perfluorinated eluents, e.g. Fluorinert® FC-75, FC-40  perfluorinated polyether, e.g. Fomblin®

Less Suitable Eluents

 diethylamine (0.1 %) (DEA)  dimethyl sulfoxide (DMSO)  slightly volatile eluents  methylene chloride  tetrahydrofuran (THF)  phosphoric dilute acid  triethylamine (0.1 %) (TEA)  trifluoroacetic acid (0.1 %) (TFA)

1. not recommended in combination with PEEK components or PEEK capillaries

P 6.1L user manual V6890

Product Information 10

Suitable Eluents

 acetate buffer solutions

 acetone at 4 °C-25 °C (39.2 °C-77.0 °F)

 acetonitrile

 benzene  chloroform  ethyl acetate  ethanol  formiate buffer solution  isopropanol  carbon dioxide (liquid 99.999 % CO

)

 methanol  phosphate buffer solutions (0.5 M)  toluol  ammoniated dilute solution  acetic acid (0.1-1 %), at 25 °C  sodium hydroxide (1 M)  water

1. valid for the specified temperature range

2. not recommended in combination with PEEK components or PEEK capillaries

Unsuitable Eluents for degassers

 Azides  benzene  Hydro fluoro solvents  halogenated hydrocarbons, e.g. Freon®  heptane  Hexafluoroisopropanol (HFIP)  hexane  Hydrofluoric acid solutions  Carbon dioxide (liquid 99.999 % CO

)

 concentrated mineral and organic acids  concentrated bases  eluents containing particles  perfluorinated eluents, e.g. Fluorinert® FC-75, FC-40  perfluorinated polyether, e.g. Fomblin®  Hydrochloric acid  dilute sodium hydroxide (1 M)

P 6.1L user manual V6890

11 Scope of Delivery

Scope of Delivery

Note: Only use original parts and accessories made by KNAUER or a com-

pany authorized by KNAUER.

 Pump P 6.1L  Power cable  AZURA accessories kit  P 6.1L accessories kit  User manual DE/EN  Installation Qualification

Safety for Users

Professional Group The user manual addresses persons who are qualified as chemical laboratory

technicians or have completed comparable vocational training. The following knowledge is required:

 Fundamental knowledge of liquid chromatography  Knowledge regarding substances that are suitable only to a limited

extent for use in liquid chromatography

 Knowledge regarding the health risks of chemicals  Participation during an installation of a device or a training by the com-

pany KNAUER or an authorized company.

If you do not belong to this or a comparable professional group, you may not perform the work described in this user manual under any circumstances. In this case, please contact your superior.

Safety Equipment When working with the device, take measures according to lab regulations

What must be taken into

account?

and wear protective clothing:

 Safety glasses with side protection  Protective gloves  Lab coat

 All safety instructions in the user manual  The environmental, installation, and connection specifications in the user

manual

 National and international regulations pertaining to laboratory work  Original spare parts, tools, and solvents made or recommended by

KNAUER

 Good Laboratory Practice (GLP)  Accident prevention regulations published by the accident insurance

companies for laboratory work

 Filtration of substances under analysis  Use of inline filters  Once they have been used, never re-use capillaries in other areas of the

HPLC system.

 Only use a given PEEK fitting for one specific port and never re-use it for

other ports. Always install new PEEK fittings on each separate port.

 Follow KNAUER or manufacturer’s instructions on caring for the colums.

P 6.1L user manual V6890

Safety for Users 12

More safety-relevant information is listed below:

 flammability: Organic solvents are highly flammable. Since capillaries can

detach from their screw fittings and allow solvent to escape, it is prohibited to have any open flames near the analytical system.

 solvent tray: Risk of electrical shock or short circuit if liquids get into the

device's interior. For this reason, place all bottles in a solvent tray.

 solvent lines: Install capillaries and tubing in such a way that liquids can-

not get into the interior in case of a leak.

 leaks: Regularly check if any system components are leaking.  power cable: Defective power cables are not to be used to connect the

device and the power supply system.

 self-ignition point: Only use eluents that have a self-ignition point higher

than 150 °C under normal ambient conditions.

 power strip: If several devices are connected to one power strip, always

consider the maximum power consumption of each device.

 power supply: Only connect devices to voltage sources, whose voltage

equals the device's voltage.

 toxicity: Organic eluents are toxic above a certain concentration. Ensure

that work areas are always well-ventilated! Wear protective gloves and safety glasses when working on the device!

Where is use of the device

prohibited?

Secure decommissioning At any time, take the device completely out of operation by either switching

Opening the Device The device may be opened by the KNAUER Technical Support or any com-

Never use the system in potentially explosive atmospheres without appropriate protective equipment. For further information, contact the Technical Support of KNAUER.

off the power switch or by pulling the power plug.

pany authorized by KNAUER only.

Signal Words

Possible dangers related to the device are divided into personal and material damage in this user manual.

Lethal injuries will occur.

Serious or moderate injuries can occur.

Minor injuries can occur.

Device defects can occur.

Decontamination

Contamination of devices with toxic, infectious or radioactive substances poses a hazard for all persons during operation, repair, sale, and disposal of a device.

Life-threatening injuries

Health danger if getting in contact with toxic, infectious or radio-active substances.

 Before disposing of the device or sending it away for repair, you are

required to decontaminate the device in a technically correct manner.

All contaminated devices must be properly decontaminated by a specialist company or the operating company before they can be recommissioned, repaired, sold, or disposed of. All materials or fluids used for decontamination must be collected separately and disposed of properly.

P 6.1L user manual V6890

13 Symbols and Signs

Decontamination Report Devices without a completed Decontamination Report will not be repaired.

If you would like to return a device to KNAUER, make sure to enclose a completed Decontamination Report with the device: http://www.knauer.net/

en/downloads/service.html

Symbols and Signs

The following symbols and signs can be found on the device, in the chromatography software or in the user manual:

Symbol Meaning

Electric shock hazard

Note maximum weight-loading of the leak tray during transport, installation and operation.

A device or system marked with CE fulfills the product specific requirements of European directives. This is confirmed in a Declaration of Conformity.

Installation

contact Phone +49 30 809727-111

Device Data For a secure transport, note the weight and dimensions of the device (see

Testing seals in Canada and the USA at nationally recognized testing centers (NRTL). The certified device or system has successfully passed the quality and security tests.

This chapter describes all preparatory steps prior to start-up. If you encounter difficulties during the installation, contact the Technical Support.

Fax +49 30 8015010

Mail support@knauer.net

Transport

Carefully prepare the device for transport or storage. If you want to return your device to KNAUER for repairs, enclose the Service Request Form which can be downloaded from our website.

Technical Data).

Bruising danger

Damage to the device by carrying or lifting it on protruding housing parts. The device may fall and thus cause injuries.

 Lift the device only centrally on the side of the housing.

Lifting Clasp the device at its side panels and lift it out of the packaging. Do not

hold onto front cover or leak tray.

P 6.1L user manual V6890

Installation 14

Location Requirements

Only if the requirements for ambient conditions of the operating environment are met, can the intended use be ensured. Details on the operating conditions can be found in the Technical Data section.

Device defect

The device overheats at exposure to sunlight and insufficient air circulation. Device failures are very likely.

 Set up the device in such a way that it is protected against exposure to

direct sunlight.

 Keep at least 15 cm clear at the rear and 5–10 cm at each side for air cir-

culation.

Space Requirements  At least 5 cm if another device is set up on one side

 At least 10 cm if further devices are set up on both sides  At least 15 cm on the rear panel for the fan.  Leave the power plug on the rear of the device accessible to be able to

disconnect the device from the mains.

General requirements  Position the device on a level and even surface.

 Protect the device against direct exposure to sunlight.  Set up the device at a location not exposed to air drafts (A/C systems).  Do not set up the device in the vicinity of other machines that cause floor

vibrations.

Cooling room It is possible to operate the pump in a cooling compartment when the fol-

lowingrequirements are fulfilled:

 The pump is switched on.  The pump is on standby modus.

Device defect

Changes of the environmental temperature cause condensation inside the device.

 Allow device to acclimate for 3 h, before connecting to power supply and

taking into operation.

Earthquake Areas If you are located in an earthquake area, use the bore holes

panels to secure the device. The bore holes are located on either right or left side panel.

1 in the side

Fig. 5 bore holes of device

Unpacking the Device

Prerequisite Check packaging for damage caused during transportation. If necessary, put

forward any claim for damages to the carrier.

P 6.1L user manual V6890

15 Installation

123

Tools Utility knife

Bruising danger

Damage to the device by carrying or lifting it on protruding housing parts. The device may fall and thus cause injuries.

 Lift the device only centrally on the side of the housing.

Process 1. Set up the package in such a way that you can read the label. Using the

utility knife, cut the adhesive tape and open the packaging.

2. Remove the foam insert. Take out the accessory kit and the manual.

3. Open the accessory kit and check the scope of delivery. In case any parts

are missing, contact the Technical Support.

4. Clasp the device from below, lift it out of the packaging and place it on

its feet. Do not hold onto the front cover.

5. Check the device for signs of damage that occurred during transport. In

case you notice any damage, contact the Technical Support.

6. Place the device in its site of operation and remove protective foil.

Next steps Store packaging and keep the included packing list for repeat orders.

Capillaries and Fittings

All tubing and capillary, which connect the components of the pump, are pre-installed. Only the solvent bottles have to be connected and the pump has to be integrated into the flow of the HPLC system.

Connecting the Solvent Bottles

To connect the solvent bottles, tubing with pre-installed solvent filters is used. The tubing is connected to the device with flangeless fittings.

Note: Do not use any tools to tighten the fittings.

Procedure Process Figure

1. Slide the flangeless fitting 1 over the tubing.

2. Slide the lock ring tubing, cap with the sealing ring

2 over the

3. Note the direction of the

lock ring, otherwise the sealing ring could be damaged. The thicker end of the lock ring must point into the direction of the fitting.

3. Manually fasten the flangeless fitting

4 to the device.

Fig. 6 Set-up of a flangeless fitting

Pre-Installed Capillaries

Pre-installed stainless steel and PEEK capillaries are color-coded according to their inner diameter.

Fig. 7 Fully assembled flangeless fit-

ting

P 6.1L user manual V6890

Installation 16

Color Material Inner Diameter

red marker stainless steel 0.1 mm

blue marker stainless steel 0.25 mm

black marker stainless steel 0.45 mm

red stripe PEEK 0.1 mm

blue stripes PEEK 0.25 mm

orange stripes PEEK 0.5 mm

Note: PEEK capillaries are not suitable for use with pure acetonitrile. Acetonitrile can cause capillaries to crack or rupture.

Integrating the Pump into an HPLC Flow System

The pump can be integrated into an HPLC flow system by connecting the pressure sensor (isocratic version) or the mixer (binary or quaternary version) and the HPLC system with capillary.

Note: To integrate the pump into a system, note the ambient conditionsfound in the sections Operating Environment and Technical Data as well asthe ambient conditions of other devices to be integrated into that system.

Tools Torque wrench

Capillary Layout

Device defect

Damage to the pump head caused by overtightened capillary fittings. Note the torque of the fittings.

 Use 5 Nm torque for stainless-steel fittings.  Use 1 Nm torque for PEEK fittings.

Isocratic pump

The figure shows the installation plan for capillary and tubing on the isocratic version of the pump. If you own a different version of the pump, see the corresponding chapter for installation instructions.

The gray lines represent the connection of the solvents to the pump and the black lines represent the connection of the piston backflushing.

Fig. 8 Installation plan for the isocratic pump

Piston backflushing The flushing solution is re-used. Since the flow path is circular, only one bot-

tle is used for the flushing solution.

Solvent flow path The pump head takes the liquid in from the bottle and conveys it to the

pressure sensor.

P 6.1L user manual V6890

17 Installation

Connecting the Pump Head to the Solvent

Prerequisites  The device has been switched off.

 The power plug has been pulled.  The front cover has been removed.

material flangeless fitting

Device defect

The pump head can get damaged when the inlet and outlet are blocked.

 Remove the cap fittings from the inlet and outlet of the pump head prior

to use.

Procedure Process Figure

1. Slide the flangeless fitting over the tubing.

2. Insert the tubing into the free inlet

1 on the bottom of the

pump head.

3. Tighten the fitting by hand.

Next Steps Integrate the pump into the HPLC flow system.

Capillary Layout

Fig. 9 Solvent line on pump head

Binary pump

The figure shows the installation plan for capillary and tubing on the binary version of the pump. If you own a different version of the pump, see the corresponding chapter for installation instructions.

The gray lines represent the connection of the solvents to the pump and the black lines represent the connection of the piston backflushing.

Fig. 10 Installation plan for the binary pump

Piston backflushing The flushing solution is re-used. Since the flow path is circular, only one bot-

tle is used for the flushing solution.

P 6.1L user manual V6890

Installation 18

Every pump head can operate with two different solvents. Both solvents are connected with the Solvent Selectionvalve into one pump head each and combined in the pressure sensor. The pres-

sure sensor is connected to the mixer. The mixer is connected to the HPLC system.

1. The solvents are conveyed

Connecting the Degasser

The degasser inlet 2 is readily connected with the solvent selectionvalve 1. The degasser outlet

10).

3 is readily connected with the pump heads (see Fig.

Solvent flow path Both solvents are connected with the

conveyed from the degasser into the pump head. From the pump head it is taken via the pressure sensor to the mixer. The mixer is connected to the HPLC system.

Note: The described flow path is not suitable for Normal Phases.

Connecting the Pump Head to the Solvent

Prerequisites  The device has been switched off.

 The power plug has been pulled.  The front cover has been removed.

material flangeless fitting

Device defect

The pump head can get damaged when the inlet and outlet are blocked.

 Remove the cap fittings from the inlet and outlet of the pump head prior

to use.

Procedure Process Figure

1. Slide the flangeless fitting over the tubing.

2. Insert the tubing into the free inlet

1 on the bottom of the

pump head.

3. Tighten the fitting by hand.

2 degasser inlet. The solvents are

Fig. 11 Solvent line on pump head

Next Steps Integrate the pump into the HPLC flow system.

Connecting the Solvent Selection Valve to the Solvent

Note: The Solvent Selection valve is readily connected to the 2-channel

degasser. The Solvent Selection valves allows selecting from two different solvents for

each solvent channel, without having to re-install the tubing. For both solvent channels A and B, one of two solvents can be selected. Solvent A is connected to inlets A1 and A2, solvent B is connected to inlets B1 and B2.

P 6.1L user manual V6890

19 Installation

2 3

Prerequisites  The device has been switched off.

 The power plug has been pulled.  The front cover has been removed.

material Flangeless fitting

Procedure

Next Steps Integrate the pump into the HPLC flow system.

Capillary Layout

Process Figure

1. Connect the tubing from the four solvent bottles to the inlets A1, A2 and B1, B2.

2. Seal inlets not in use with cap fittings.

Fig. 12 Solvent Selection valve with

cap fitting

Quaternary Pump

The figure shows the installation plan for capillary and tubing on the quaternary version of the pump. If you own a different version of the pump, see the corresponding chapter for installation instructions.

The gray lines represent the connection of the solvents to the pump and the black lines represent the connection of the piston backflushing.

Fig. 13 Installation plan for the quaternary pump

Piston backflushing The flushing solution is re-used. Since the flow path is circular, only one bot-

tle is used for the flushing solution.

Connecting the Degasser

The degasser outlet 2 is readily connected to the valve block 1 (see fig.

13).

Solvent flow path The four solvents are connected with the

ture is conveyed from the degasser via the valve block into the pump head. From the pump head it is taken to the mixer. The mixer is connected to the HPLC system.

Note: The described flow path is not suitable for Normal Phases.

P 6.1L user manual V6890

3 degasser inlet. The solvent mix-

Installation 20

Connecting the Pump Head to the Solvent

Prerequisites  The device has been switched off.

 The power plug has been pulled.  The front cover has been removed.

Material Flangeless fitting

Device defect

The pump head can get damaged when the inlet and outlet are blocked.

 Remove the cap fittings from the inlet and outlet of the pump head prior

to use.

Procedure Process Figure

1. Slide the flangeless fitting over the tubing.

2. Insert the tubing into the free inlet

1 on the bottom of the

pump head.

3. Tighten the fitting by hand.

Fig. 14 Solvent line on pump head

Next Steps Integrate the pump into the HPLC flow system.

Connecting the 4-Channel Degasser to the Solvent

The 4-channel degasser contains four degassing chambers. Each degassing chamber has an inlet and an outlet on the front of the pump.

Device defect

Very high pressures can damage the degasser membrane. The membrane can withstand a maximum pressure of 7 bar.

 Never connect the degasser to the pump outlet.

Prerequisites  The device has been switched off.

 The power plug has been pulled.  The front cover has been removed.

Material Flangeless fitting

P 6.1L user manual V6890

21 Installation

Procedure

Next Steps Integrate the pump into the HPLC flow system.

Process Figure

1. Connect the tubing from the four solvent bottles to the inlets A, B, C, and D of the degasser

(see

2).

2. Seal inlets not in use with cap fittings.

Fig. 15 4-channel degasser with

Connecting the Valve Block

Note: Note the instructions on how to install the flangeless fitting (see

“Capillaries and Fittings” on page 15). The valve block inlets

has to be changed, e.g. you want to install different capillary, note that at least two of the outer flangeless fittings on the valve block are loosened in order to be able to install connection in the center by hand.

1 are pre-installed. If the central outlet connection

valve block

Connecting the Piston Backflushing

The piston backflushing removes salts and other substances from the area behind the pump head seals. To do this, connect a bottle with flushing solution to the flush pump and the piston backflushing. The silicone tubing between the piston backflushing and the flush pump are pre-installed.

Designation Inlet and outlet of the flush pump are located on the front of the device. The

flush pump is inside of the device and not visible from the outside.

Fig. 16 Inlet symbol Fig. 17 Outlet symbol

Prerequisite  The pump has been set-up at the site of operation.

 The pump has been switched off.

Material Silicone tube

P 6.1L user manual V6890

Installation 22

Procedure

Process Figure

1. Plug one silicone tube the inlet

and connect to the flushing solution bottle.

2. Plug a second silicone tube onto a vacant capillary connector of the flush pump and connect

to the flushing solution bottle.

3 of the flush pump

2 onto

Connecting the Leak Management

The leak management consists of the leak sensor and the drainage system (funnels, hoses, nozzles). The drainage system ensures that escaping liquids flow into a waste bottle. When leaks are registered by the leak sensor, the LED flashes red. Both the device and the data acquisition via chromatography software are stopped.

Prerequisite The front cover has been removed.

Process Process Figure

1. Carefully push the funnel the center opening of the capil-

lary guide

2. Push the long ending of the first nozzle

3. Connect the nozzle and the funnel.

4. Push the other end of the hose onto the nozzle tray.

4 into the hose 3.

5 of the leak

1 into

Fig. 18 Funnel and capillary guide

Fig. 19 Hose and nozzle

Fig. 20 Hose connected to device

P 6.1L user manual V6890

23 Installation

Process Process Figure

5. For the bottom device, push the short end of the nozzle the opening in the collection

point of the leak tray.

6. Connect the hose to the nozzle and lead the second ending to the waste bottle.

7. Place the waste bottle below the bottom device.

Next steps Attach the front cover.

Control

The pump can be operated in two ways:

 Via the pin head  as part of a LAN, via the LAN connector of the router

All connectors for external control are located on the rear side of the pump.

Legend

1 Service interface

6 into

Fig. 21 Leak tray with nozzle

2 Events connector

3 LAN connector

4 Remote connector

Process 1. On the computer, go to the control panel and check the LAN properties.

Fig. 22 Connectors on rear side

Computer Control

This section describes how to set up an HPLC system in a local area network (LAN) and how a network administrator can integrate this LAN into your

company network. The description applies to the operating system Windows and all conventional routers.

Note: To set up a LAN, we recommend to use a router. That means the following steps are required:

2. Hook up the router to the devices and the computer.

3. On the computer, configure the router to set up the network.

4. Install the chromatography software from the data storage device.

5. Switch on the device and run the chromatography software.

Configuring the LAN Settings

The LAN uses only one server (which is normally the router) from that the devices automatically receive their IP address.

P 6.1L user manual V6890

Installation 24

Prerequisite  In Windows, power saving, hibernation, standby, and screen saver must

be deactived.

 In case you use an USB-to-COM box, the option "Allow the computer to

turn off ths device to save power" in the devicemanager must be deactivated for all USB hosts.

 Only for Windows 7: For the network adapter, the option "Allow the

computer to turn off this device to save power" in the Device Manager must be deactivated.

Process 1. In Windows 7 choose Start

ter.

2. Double-click on LAN Connection.

3. Click on the button Properties.

4. Select Internet Protocol version 4 (TCP/IPv4).

5. Click on the button Properties.

6. Check the settings in the tab General. The correct settings for the DHCP client are: a) Obtain IP address automatically b) Obtain DNS server address automatically

7. Click on the button OK.

Connecting the Cables

A router 3 has several LAN ports 2 and one WAN port 4 that can be used to integrate the LAN into a wide area network (WAN), e.g. a company net-

work or the Internet. In contrast, the LAN ports serve to set up a network from devices operating the HPLC system separately from the company network.

1 and a computer 5. To avoid interference, we recommend



Control Panel  Network and Sharing Cen-

Prerequisite  The computer has been switched off.

You will find patch cables for each device and the router in the accessories kit. To connect the router to a WAN, an additional patch cable is required, which is not supplied within the scope of delivery.

 There is a patch cable for each device and the computer.

Process 1. Use the patch cable to connect the router and the computer. Repeat this

step to connect all devices.

2. Use the power supply to connect the router to the mains power system.

Configuring the Router

The router is preset at the factory. You will find a label at the bottom side of

Process 1. To open the router configuration, start your Internet browser and enter

the router, on which IP address, user name, and password are printed. These information help to open the router configuration.

the IP address (not for all routers).

2. Enter user name and password.

3. Configure the router as DHCP server.

P 6.1L user manual V6890

25 Installation

4. In the router configuration, check the IP address range and make changes if necessary.

Result Once the router has assigned IP addresses to all devices, the chromatogra-

phy software can be used to remotely control the system.

Integrating the LAN into a Company Network

A network administrator can integrate the LAN into your company network. In this case you use the WAN port of the router.

Prerequisite There is a patch cable for the connection.

Process 1. Check that the IP address range of the router and of the company net-

work do not overlap.

2. In case of an overlap, change the IP address range of the router.

3. Use the patch cable to connect the router WAN port to the company network.

4. Restart all devices, including the computer.

Controlling Several Systems Separately in a LAN

Devices connected to a LAN communicate through ports, which are part of the IP address. If more than one HPLC system is connected to the same LAN and you plan on controlling them separately, you can use different ports to avoid interference. Therefore, the port number for each device must be changed and this same number must be entered into the device configuration of the chromatography software. We recommend to use the same port number for all devices in the same system.

Note: The port is set to 10001 at the factory. You must use the same numbers in the device configuration of the chromatography software as in the device, otherwise the connection fails.

Process 1. Find out port number and change it on the device.

2. Enter the port number in the chromatography software.

Result The connection is established.

Setting a Static IP Address

Note: Please inform yourself about the IT safety standards valid for your lab-

oratory, before changing the LAN settings. The device is factory set to a dynamic IP address (DHCP). To ensure a per-

manent LAN connection between the chromatography software and the device, we recommend to set a static IP address for certain applications.

Prerequisites  The device has been switched on.

 Mobile Control has been installed and started.  The connection between the Mobile Control and the device has been

established.

Note: You find further information on LAN settings in the chapter Device Settings of the Mobile Control User Manual.

Procedure 1. In the Mobile Control, choose Settings .

2. On the General tab, choose the device name.

3. Under Network Settings, choose the setting Static

P 6.1L user manual V6890

Installation 26

4. Enter the IP address into the text box IP Address 2.

Next steps In case necessary, go back to the original setting with the [Reset] button that

Legend

1 display

2 Remote terminal strip

5. If necessary, change the subnet mask and the gateway

6. Click in the top right corner.

7. Restart the device.

can be found under Settings > General > Network Settings > LAN Settings.

Pin Header Connectors

Remote Terminal Strip

 For receiving start, control, and error signals from external devices  For sending start, control and error signals to external devices

Fig. 23 Remote terminal strip

Signal Explanation

Analog GND Reference point of the voltage at the signal inputs.

Analog out Voltage range 0 – 5 V, scalable

Analog in Voltage range 0 – 10 V

10 V according to maximum flow rate

Start GND Reference point of the voltage at the signal inputs.

P 6.1L user manual V6890

27 Installation

Signal Explanation

Start OUT TTL Output

Levels:

 passive 5 V  active 0 V

Start IN TTL Input

 Low active

Secure switching threshold at least 10 mA After receiving a signal (short-circuit to ground) from an external device, the device starts. If controlled with software, an electronic trigger is send through the LAN.

Error OUT TTL Output

Levels:

 passive 5 V  active 0 V

Error IN TTL Input

 Low active

Secure switching threshold at least 10 mA After receiving a signal (short-circuit to ground) from an external device, an error message appears and the device stops.

Error GND Reference point of the voltage at the signal inputs.

+24V Event-controlled switching of 24 V

against GND Protection: 24 V-200 mA

+5V Provides a voltage of 5 V with respect to GND.

This makes it possible to supply a consumer that is switched by an EVENT. Protection: 5 V–50 mA

GND Reference point of the voltage at the signal inputs.

Events Connector

For test purposes or in some other cases, it can make sense to manually enter these signals:

 sending control signals (Events) to external devices  opening and closing contacts  Activating 500 ms pulses

P 6.1L user manual V6890

Legend

1 Display

2 Events terminal strip

Installation 28

Fig. 24 Events Remote Connector

Assignment connection Function

1TTL TTL Output

Levels:

 passive 0 V  active 5 V

Pulse:

 5 V for at least 1000 ms

2TTL TTL Output

Levels:

 passive 0 V  active 5 V

Pulse:

 5 V for at least 1000 ms

3OC TTL-compatible output

Levels:

 passive 5 V  active 0 V

Pulse:

 5 V for at least 1000 ms

GND Reference point of the voltage at the signal inputs.

4TTL TTL Output

Levels:

 passive 0 V  active 5 V

Pulse:

 5 V for at least 1000 ms

P 6.1L user manual V6890

29 Installation

Assignment

connection Function

5TTL TTL Output

Levels:

 passive 0 V  active 5 V

Pulse:

 5 V for at least 1000 ms

6OC TTL-compatible output

Levels:

 passive 5 V  active 0 V

Pulse:

 5 V for at least 1000 ms

GND Reference point of the voltage at the signal inputs.

Relay Contact

The contact is on a floating basis. Its setting depends on the settings in the software. Steady-rate signal:

 passive = open relay contact  active = closed relay contact

Pulse:

 Closed relay contact for at least 1000 ms

Permissible load of the relay contact: 1 A/ 24 V DC

Relay Contact

The contact is on a floating basis. Its setting depends on the settings in the software. Steady-rate signal:

 passive = open relay contact  active = closed relay contact

Pulse:

 Closed relay contact for at least 1000 ms

Permissible load of the relay contact: 1 A/ 24 V DC

Connecting Cables to the Terminal Strip

To control one device through another, you use the multi-pin connector. To use remote control, you have to connect cables to the terminal strip (both included with delivery). The single ports are used to exchange control signals.

Prerequisite  The device has been turned off.

 The power plug has been pulled.

Tools Depressor tool

Electronic defect

Connecting cables to the multi-pin connector of a switched on device causes a short circuit.

 Turn off the device before connecting cables.  Pull the power plug.

P 6.1L user manual V6890

Initial Start-Up 30

Electronic defect

Electrostatic discharge can destroy the electronics.

 Wear a protective bracelet against electrostatic discharge and ground.

Process 1. Push the depressor tool

upper small opening on the front of the terminal strip

2. Lead the cable into the opening

1into an

2 below the inserted depressor

tool.

3. Remove the depressor tool.

Next steps Check if the cables are firmly attached. Push the terminal strip onto the

multi-pin connector. Finish the installation. Then put the device into operation.

Analog Control

Analog ports serve for exchanging analog control signals. Reference point for the signals is the connector GND.

 OUT: Device sends signal.  IN: Device receives signal.

Power Connection

Note: The nominal capacity of the connected devices must be maximum

50 % of the power supply to account for larger inrush currents when switching on the modules.

The maximum power input is 100 W.

Prerequisites  The electrical power supply at the installation site must be connected

 The power must be free from ripple, residual current, voltage peaks and

 The connectors for the mains voltage are grounded accordingly.  The device receives sufficient power with reserve capacity

The device is intended for use with AC power networks of 100-240 V.

Power Cable Only the supplied power cable is to be used to connect the device to the

power supply. Replace defective power cables only with accessories from KNAUER. Only use power cables with a permission for use in your country.

Power Plug Make sure that the power plug on rear of the device is always accessible, so

that the device can be disconnected from the power supply.

Next Steps Finish the installation and connect the device to the mains.

Initial Start-Up

directly to the nearest main power line.

electromagnetic interference.

Note: Prior to switching on the pump, you should bleed it to remove air from capillary and tubing.

Start-Up

Use this checklist to check if the detector is ready for initial start-up:

 Device is positioned in the correct location.  The power plug has been connected.

P 6.1L user manual V6890

31 Initial Start-Up

If the device is part of an HPLC system, you should also note the following:

 The network connection to the router is established  The chromatography software has been installed by KNAUER or a com-

pany authorized by KNAUER.

 The capillaries have been connected.

Pump Head

To bring a newly installed pump head into service securely, it should run in prior to its first operation. For this purpose, set the following parameters:

Note: If pump heads are used for normal phase applications, only use isopropanol as solvent.

Pump head 5 ml, 10 ml Pump head 50 ml

Eluent H

Back pressure 30 MPa (min > 15 MPa) 12 MPa (min > 8 MPa)

Flow rate 2-5 ml/min 5-20 ml/min

Run time 30 min 90 min

Drag Capillary with inner diameter of

O (degassed) or ethanol H2O (degassed) or ethanol

Capillary with inner diameter of

0.1 mm length 2 m

Back pressures higher than 8 MPa are generated by back pressure regulators.

0.1 mm length 1 m

Bleeding the Pump

Before the pump can be used, it must be bleeded.

Mode “Flow on” The pump can only be bled when switched on because the syringe can only

take in liquid in "Flow on" mode. Only then are the valves of a binary pump opened.

Quick suction process For the binary pump, the bleeding process may take a while during initial

start-up because the solvent tubes are filled with liquid for the first time.

Prerequisites  The installation has been completed.

 The capillaries and tubings have been connected.  The pump has been switched on.

Tools Syringe with Luer lock

Column defect

Damage to the column due to bleeding.

 Open the venting screw.  Remove the column.

P 6.1L user manual V6890

Operation 32

Procedure

Next Steps Start the pump using the software (option: Purge) with a medium flow rate

Result After bleeding, excess air is removed from the pump head and the capillary.

Process Figure

1. Open the venting screw pressure sensor.

2. With the syringe, extract fluid through the bleed port

(50 % of the maximum flow). If the extracted fluid flows continuously, stop suction and close the venting screw.

1of the

Fig. 25 Venting screw of the pressure

sensor

Switch-On

Note: Prior to switching on the pump, you should bleed it to remove air

from capillary and tubing. After switching on the pump, the piston backflushing automatically flushes

for 15 seconds.

Prerequisites The installation has been completed.

Procedure 1. Connect the power cable of the device to the power supply.

Next Steps Operate the pump.

Operation

Device defect

Changes of the environmental temperature cause condensation inside the device.

 Allow device to acclimate for 3 h, before connecting to power supply and

taking into operation.

Component defect

Damage to the pump head in case it runs dry.

 Ensure that liquid runs through pump head and piston backflushing.

2. Switch the power supply on.

3. Wait until the pump has completed the self-test.

4. Start the pump at a medium flow rate.

There are several options for controlling the device:

 with chromatography software  with Mobile Control

Note: It is not possible to use 2 control methods simultaneously. If the device is connected to the software, it cannot be controlled via Mobile Control.

Operating with Chromatography Software

To operate the device with software, you have to establish a connection between the LAN port and a computer.

P 6.1L user manual V6890

33 Operation

Devices can be controlled with e. g. OpenLAB EZChrom edition version A.04.05 or higher or ClarityChrom version 5.0.2 or higher. You find a detailed description on chromatography software in a corresponding user manual.

Operating with Mobile Control

The Mobile Control is an app which can be installed on your computer or tablet. To control the device using the Mobile Control, connect the computer or tablet with operating system Windows 8 to a wireless LAN router. The firmware version of your P 6.1L must be V01.02 or higher. You find a detailed description on the Mobile Control in its accompanying user manual.

Meaning of the LEDs

On the LED panel there are three LEDs 1 and a standby button 2. The figure shows the LED panel when the device is switched off.

The LEDs can have different colors depending on the operating conditions.

Color Operating condition Operation

Left LED red flashing Error status Check the system.

red Fatal error Restart the device.

green Program or sequence

is running/was loaded.

Center LED does not light Not ready

green flashing Equilibration Wait until the device is

green Ready

Right LED, Power Status green Power on

Shortly press the switch to deactivate the error message.

If the fatal error status remains, contact Service.

ready.

Standby To activate the standby, keep the switch pressed for 5 seconds.

blue Standby Press the switch to end

the standby.

Note: Malfunctioning system after repeated standby possible. After repeatedly using the standby, switch off the power switch and back on again, to reset the data storage.

P 6.1L user manual V6890

Functional Tests IQ and OQ 34

Functional Tests IQ and OQ

Installation Qualification

(IQ)

Operation Qualification

(OQ)

Test Intervals To make sure that the device operates within the specified range, you

The customer may request the Installation Qualification, which is free of charge. In case of a request, the Technical Support of KNAUER or from a provider authorized by KNAUER performs this functionality test during the installation.

The Installation Qualification is a standardized document that comes as part of the delivery and includes the following:

 confirmation of flawless condition at delivery  check if the delivery is complete  certification on the functionality of the device

The Operation Qualification includes an extensive functionality test according to KNAUER standard OQ documents. The Operation Qualification is a standardized document and free of charge. It is not part of the delivery, please contact the Technical Support in case of request.

The Operation Qualification includes the following:

 definition of customer requirements and acceptance terms  documentation on device specifications  device functionality check at installation site

should test the device regularly. The test intervals are dependent on the usage of the device.

Execution The test can be carried out either by the Technical Support of KNAUER or

from a provider authorized by KNAUER (for a fee).

P 6.1L user manual V6890

35 Troubleshooting

Troubleshooting

First Measures 1. Check all cabling.

2. Check all screw fittings.

3. Check whether air has gotten into the supply lines.

4. Check device for leaks.

5. Pay attention to system messages.

LAN

Go through the following steps, in case no connection between the computer and the devices can be established. Check after each step if the problem is solved. If the problem cannot be located, call the Technical Support.

1. Check the status of the LAN connection in the Windows task bar:

 Connected

 Connection not established

If no connection was established, test the following:

 Is the router switched on?  Is the patch cable connected correctly to the router and the com-

puter?

2. Check the router settings:

 Is the router set to DCHP server?  Is the IP address range sufficient for all the connected devices?

3. Check all connections:

 Are the patch cable connected to the LAN ports and not the WAN

port?

 Are all cable connections between devices and router correct?  Are the cables plugged in tightly?

4. If the router is integrated into a company network, pull out the patch cable from the WAN port.

 Can the devices communicate with the computer, even though the

router is disconnected from the company network?

5. Turn off all devices, router, and computer. Firstly, turn on the router and secondly turn on the devices and the computer.

 Has this been successful?

6. Replace the patch cable to the device with that no connection could be established.

 Has this been successful?

7. Make sure that the IP port of the device matches the port in the chromatography software.

Possible Problems and Solutions

Error Solution

Device cannot be switched on

When purging, the pump switches off.

P 6.1L user manual V6890

Inspect the power cable to ensure that it is plugged into the power supply.

Check if the venting screw on the pressure sensor is turned up.

Troubleshooting 36

Pump does not transport solvent

Pressure and flow rate variations

Pump head leaks  Inspect the inlet and outlet screw fittings of the

 Purge the pump head to remove the air bub-

bles.

 Clean the check valves.  Exchange the check valves.  If the pump head seals are defective, solvent

enters the piston backflushing; inform the technical support.

 Exchange the pump head

 Purge the pump head to remove the air bub-

bles.

 Always tighten the inlet screw fittings and out-

let screw fittings on the pump head with a torque wrench (see ’Checking the Fittings’ auf Seite 41.).

 Clean the check valves.  Exchange the check valves.  Exchange the pump head  Inform technical support.

pump head.

 If the seals are defective, eluent enters the pis-

ton backflushing; inform the technical support.

 Exchange the pump head

Flow rate is not correct

System error Switch off the device to reset the device's data

Check the following options:

 Check the data for the solvent compressibility  Clean the check valves.  Exchange the check valves.

storage and restart it afterwards.

System Messages

If other system messages are displayed besides those listed below, please turn the device off and then on. Inform the technical support in case the system message repeats.

The system messages are in alphabetical order:

System message

A "A line with this time

already exists: edit the time please"

C "Cannot delete

active program/link"

Correct the time entry.

Pause the program/link. Only then can the link and, subsequently, the program used in the link be deleted.

"Cannot edit program from the running link"

“Cannot initialize LAN”

P 6.1L user manual V6890

Pause the link. Afterwards, the program can be changed.

Check cables and connections in local area network.

37 Troubleshooting

System message

"Cannot operate with an empty link"

"Cannot purge during the run"

"Cannot read data from FRAM"

"Cannot start time table"

"Cannot use nonexisting component"

"Cannot write data on FRAM"

"Component settings not compatible with gradient setup"

E ”Error input acti-

vated”

Create a link.

End method and start purging.

Switch the device off and on. Inform the technical support in case the system message repeats.

Check the data on the device's display or in the opened program.

Check if the channels to be mixed are set correctly in the Setup section of the device or in the Instru- ment Setup of the chromatography software.

Switch the device off and on. Inform the Technical Support in case the system message repeats itself.

Change the setup settings or change the gradient in the program or in setup.

Eliminate the short circuit connected to ‘Error In’.

F "Flow max in the

program is not compatible with the current pump head"

G "GUI communica-

tion failed"

H "HPG B component

not present"

"HPG B: Command timeout”

"HPG B: incompatible pump head type"

"HPG B: Service active"

I ”Instrument remote

controlled”

"Invalid index in time table"

When entering the flow, note the maximum flow of the pump head and only enter values inside this range.

Switch the device off and on. Inform the technical support in case the system message repeats itself.

This entry is not executable. Quit software.

Change the entry in the program line.

"Invalid line number"

"Invalid link" Create a link or use an existing link.

L "Leak sensor not

present"

Switch the device off and then on. If the leak sensor cannot be found, contact the Technical Support.

P 6.1L user manual V6890

Troubleshooting 38

System message

“Leak was detected” Switch off the device. Remove the leak and start

the device afterwards.

"Line in time table is

Edit the program line.

empty"

"Link is running" Wait until the link has been completed, then

change the link or delete it.

M "Maximum pressure:

System stopped"

 Check whether the connected capillary and

connectors are clogged.

 Adjust the maximum pressure limit.  Restart the system.

"Minimum pressure: System stopped"

 Increase the pressure or adjust the lower pres-

sure limit.

 Restart the system.

"Motor failure: max current"

Switch the device off and on. Inform the technical support in case the system message repeats itself.

"Motor failure: position error"

"Motor failure:

N "No link available.

Create a link and edit it.

Pls edit link first"

"No link available"

"No valid pump head type detected; 50 ml pump head is set."

 Switch the device off and on.  Check whether a pump head with RFID recog-

nition has been installed.

 Repeat the automatic configuration step in the

chromatography software.

 Remove pump head, clean it and install it

again.

"Non-existing component is set to non-

Switch on the channel or edit the data using the chromatography software.

0 value"

"Not enough space to store link"

 Check the pump  Check the number of program lines - a maxi-

mum of 100 program lines are possible.

"Not enough space to store program"

 Check the pump  Check the number of program lines - a maxi-

mum of 100 program lines are possible.

"Not in HPG mode" Select HPG mode.

P "Pressure max in the

program is not compatible with the current pump head"

P 6.1L user manual V6890

Note the maximum pressure of the pump head.

39 Troubleshooting

System message

"Program does not

Create and edit a program. exist, please edit the program number"

"Program is running" Quit program or wait until program has been

completed.

"Pump head type: head data uninitialized"

 Switch the device off and on

 Check whether a pump head with RFID recog-

nition has been installed

 Repeat the automatic configuration step in the

chromatography software

 Remove pump head, clean it and install it again

"Pump head type: read failed"

 Switch the device off and on

 Repeat the automatic configuration step in the

chromatography software

 Remove pump head, clean it and install it again

 Inform the Technical Support in case the sys-

tem message repeats

"Pump head type: RFID hardware not

Pump head without RFID detection: If necessary,

replace pump head. present or failed"

"Pump head type: write failed"

S "SetPoint in the pro-

gram is not compatible with the current PH PMax"

T "This link is used in

WAKEUP"

"This program is used in a link"

"This program is used in WAKEUP"

"Too many lines in program"

 Switch the device off and on

 Repeat the automatic configuration step in the

chromatography software

 Remove pump head, clean it and install it again

 Inform the Technical Support in case the sys-

tem message repeats

Note the maximum pressure of the pump head.

First quit or delete wakeup program (wu = Wake

Up), then edit or delete link.

First pause or delete the link, then edit or delete

data by means of the chromatography software.

First quit or delete wakeup program (wu = Wake

Up), then edit or delete data by means of the

chromatography software.

Check the number of program lines.

A maximum of 100 program lines are possible.

U "Unable to attain

min. flow setpoint"

"Unable to attain pressure setpoint"

P 6.1L user manual V6890

Confirm, pump continues running

The entered pressure cannot be achieved with the

maximum flow set in the Constant Pressure mode.

 Check for leaks.

 Increase the upper flow level.

 Reduce the working pressure.

Maintenance and Care 40

System message

"Unknown pump head type"

W "Wake up time

already passed"

Maintenance and Care

Organic eluents are toxic above a certain concentration. Ensure that work areas are always well-ventilated! When performing maintenance tasks on the device, always wear safety glasses with side protection, protective gloves, and an overall.

All wetter components of a device, e. g. flow cells of detectors or pump heads and pressure sensors for pumps, have to be flushed with a suitable flushing solution before being maintained, disassembled or disposed.

Opening the Module The device may only be opened by the KNAUER Technical Support or any

company authorized by KNAUER.

Electronic defect

Performing maintenance tasks on a switched on device can cause damage to the device.

 Switch off the device  Pull the power plug.

 Check the pump head.

 Check whether a pump head with RFID recog-

nition has been installed

 Inform the Technical Support in case the sys-

tem message repeats

Correct the entry for date or otherwise time.

Device defect

Leaks can damage the device.

 If leaks occur after maintenance or assembly, replace the capillary con-

nections with new ones.

Users may perform the following maintenance tasks themselves:

 Replacing the pump head  Replacing the check valves of the pumps  Replacing the inline filter of the pressure sensor  Replacing the mixer

Proper maintenance of your HPLC device will ensure successful analyses and reproducible results.

Maintenance Contract

The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract:

 Opening the device or removing housing parts

Maintenance Intervals

Operating Hours The Control Unit, the Mobile Control and the different software products

(e.g. ClarityChrom® or OpenLAB®) enable you to display or read out the operating time of the pump. You find a detailed description on how to display or read out GLP data in the respective user manuals.

P 6.1L user manual V6890

41 Maintenance and Care

Operating Hours Measures

1000  Check the torque of the screw fittings

 Clean the pistons of the pump

 Check the check valves of the pump head

5000  Replace all seals

 Clean the check valves of the pump head

10000  Replace the spare parts in the pump head

 Replace the check valves of the pump head

Cleaning and Caring for the Device

Device defect

Intruding liquids can cause damage to the device.

 Place solvent bottles next to the device or in a solvent tray.  Moisten the cleaning cloth only slightly.

All smooth surfaces of the device can be cleaned with a mild, commercially available cleaning solution, or with isopropanol.

Checking the Fittings

Check if all fittings are tight. In case you find fittings that are not tight, tighten them up. Not the torque applicable for each fitting in order to not damage any components.

Material & Size Pump head inlet Pump head outlet

Stainless steel 5 ml 7.5 Nm 10 Nm

Stainless steel 10 ml, 50 ml

Inline Filter Fittings Torque

Stainless steel fittings 7.5 Nm

PEEK fittings 3.5 Nm

Capillary Screw Fittings Torque

Stainless-steel fittings 5 Nm

PEEK fittings 1 Nm

7.5 Nm 7.5 Nm

Flushing the Pump

Generally, the pump and all its components (valves, degasser) should be flushed after each operation. Also, flush the pump to clean the tubing before changing the solvent and to remove air bubbles in the capillary and tubing. If buffer solution has been employed, note that the buffer solution and the flushing solution are compatible.

 When buffers have been used, flush with water.  When aggressive solvents have been used, flush with isopropanol.

Note: For applications wirth Normal Phase, only use Isopropanol as flushing solution.

P 6.1L user manual V6890

Maintenance and Care 42

Device defect

Residuals chemicals can damage the pump head if they are not being removed before storage.

 For reuse, flush the pump head and fill it with isopropanol.  Before storage, seal the inlets and outlets with cap fittings.

Auxiliary Material flushing solution, silicone tubing

Procedure Process Figure

1. Immerse the solvent tubing into

the flushing solution.

2. Plug a silicone tube onto the

venting nozzle sensor.

3. Open the venting screw

4. Start the pump at a medium

flow rate.

2 of the pressure

Next Steps Bring the pump into operation again.

Putting the Pump Out of Operation

The pump is designed for the use of different solvents. In case the pump has not been used for several weeks, solvent residues may cause damage. It is therefore recommended that all components of the pump be flushed, the solvent used be removed completely and that all components and tubes of the pump be filled with isopropanol. All open connectors should be sealed. Capillary and tubing which connects single components of the pump should not be removed.

Prerequisites  The pump has been rinsed.

 The pump has been switched off and disconnected from the power sup-

ply.

Tools Open-end wrench, size 10

Procedure Process

1. Remove the solvent tubing and seal all open connectors with cap fit-

tings.

2. Remove the pump from the HPLC flow system and seal the open con-

nector of the pressure sensor (isocratic version) or the mixer (binary or quaternary version) with cap fittings.

Fig. 26 Pressure sensor

Next Steps Select a storage location according to the requirements, which are listed in

the according chapter of this manual.

Storage

Pay attention that all hoses and capillaries have been emptied or filled with flushing solution (e. g. isopropanol) before storage. To prevent algae formation, do not use pure water. Close all inputs and outputs with cap fittings.

P 6.1L user manual V6890

43 Maintenance and Care

The device can be stored under the following ambient conditions:

 temperature range 4-40 °C (39.2-104 °F)

 air humidity below 90 %, non-condensing

Disconnecting the Power Supply

Prerequisites The device has been switched off.

Procedure

Next Steps Disconnect all remaining electrical connections, remove the pump head and

Process

1. Pull the power plug out of the socket and afterwards out of the device.

2. Pack the power cable together with the device.

pack the device for transport or storage.

Preparing the Pump Head for Storage

Prerequisites The power plug has been pulled.

Auxiliary Material  Syringe

 Flushing solution

Device defect

Residuals chemicals can damage the pump head if they are not being removed before storage.

 For reuse, flush the pump head and fill it with isopropanol.  Before storage, seal the inlets and outlets with cap fittings.

Procedure Process

1. Fill the flushing solution into a syringe and inject into the capillary at

the pump head inlet. Leave it for 5 minutes.

2. Flush with suitable flushing solution.

3. Flush the pump head with isopropanol.

4. Seal the inlets and outles with cap fittings.

Next Steps Dismount the pump head.

Dismounting the Pump Head

Prerequisites  The pump head has been purged.

 The tubes at the inlet and outlet have been removed.  The pump head's inlet and outlet have been sealed with cap fittings.

Tools  Allen wrench 3 mm

 Open-end wrench, size 1/4"  Open-end wrench, size 13

Chemical burns

Aggressive or toxic solvent residue can irritate the skin.

 Wear protective gloves.  Flush the pump head before exchanging it.

P 6.1L user manual V6890

Maintenance and Care 44

1 2

Device defect

Residuals chemicals can damage the pump head if they are not being removed before storage.

 For reuse, flush the pump head and fill it with isopropanol.  Before storage, seal the inlets and outlets with cap fittings.

Component defect

Damage to the pistons if they tilt.

 Undo or tighten diagonally opposite screws evenly by one turn each at a

time.

Procedure Process Figure

1. Loosen the capillary connectors

1 at the outlet. Remove the

capillary.

1. Fixate the outlet fitting

an open-end wrench (size 13).

2. Loosen the fitting

open-end wrench (size 1/4").

3. Remove the solvent lines 2

from the solvent inlets.

4. Disconnect the hoses of the pis-

ton backflushing flush pump and the pump head.

1 from the

1 with

2 with an

Fig. 27 Outlet screw fitting

5. Using the Allen wrench, unscrew

the 4 screws one turn.

6. Fixate the pump head with your

hand and remove the screws.

7. Lift off the pump head.

1 subsequently by

P 6.1L user manual V6890

Fig. 28 Tubings piston backflushing

Fig. 29 Screws

45 Maintenance and Care

Next Steps Maintain the check valves or install a new pump head.

Check Valves

Clogged check valves do not open and close properly. They cause pressure fluctuations and irregular flow. If it is impossible to clean the check valves, replace the whole unit.

Procedure  Removing the Check Valves

 Cleaning the Check valve  Installing the Check Valve

Removing the Check Valves

The pump head is equipped with two check valves.

Prerequisites  The pump head has been purged.

 The capillaries and tubings have been removed.  The pump head has been removed.

Tools  Open-end wrench, size 13

Procedure

Next Steps Clean the check valves.

Process Figure

1. Unscrew and remove the capil-

lary connector

2. Loosen the outlet fitting

the open-end wrench..

3. Remove the first check valve.

4. Loosen the inlet fitting

the open-end wrench..

5. Remove the second check valve.

Cleaning the Check Valve

The check valves are not disassembled for cleaning but they are cleaned as a unit.

2 with

3 with

Fig. 30 Check valve in pump head

Prerequisites Both check valves have been removed.

Procedure Process

1. Place each check valve into a beaker with solvent, e.g. isopropanol.

2. Put the beaker with the check valve in an ultrasonic bath for at least 10

minutes.

3. Let the check valves dry afterwards.

Next Steps Insert both cleaned check valves.

Installing the Check Valve

Insert the check valves in the direction of the flow. The notch of the check valve points downward.

P 6.1L user manual V6890

Maintenance and Care 46

Normal phase Insert the check valves in the direction of the flow. The arrow on the check

valve points upward.

Prerequisites The check valves have dried.

Component defect

Damaging the threads of components caused by overtightened fittings. Pay attention to the torque values.

 Use 5 Nm for stainless steel fittings.  Use 1 Nm for PEEK fittings.

Procedure Process Figure

1. Insert the check valves

such a way that the notch of the check valve

ward.

2. Manually screw in inlet and out-

let fittings and tighten them with a torque wrench and the respective torque.

Next Steps Re-install the pump head.

2 points down-

1 in

Fig. 31 Check valve

Inline Filter on the Pressure Sensor

Clogged inline filters inside of the pressure sensor can cause pressure fluctuations and irregular flow. Inline filters are not cleaned but exchanged as an assembly.

Procedure 1. Remove the capillary below the inline filter of the pressure sensor.

2. Loosen the fitting of the inline filter and remove manually.

3. Insert a new inline filter cartridge.

Removing the Inline Filter

Below the pressure sensor, you find the fitting of the inline filter.

Prerequisites The pump has been rinsed.

Tools  Open-end wrench, size 1/4"

 Open-end wrench, size 13

Procedure Process Figure

1. Fixate the outlet fitting

an open-end wrench (size 13).

2. Loosen the fitting

inline filter sensor

wrench.

2 of the pressure

1 with the open-end

2 with

3 below the

Fig. 32 Loosen the capillary.

P 6.1L user manual V6890

47 Maintenance and Care

Procedure Process Figure

3. Loosen the fitting of the inline

filter

1 with the open-end

wrench (size 13) and removemanually.

4. Remove clogged inline filter car-

tridge.

Next Steps Insert the new inline filter cartridge.

Inserting the New Inline Filter Cartridge.

Fig. 33 Loosen the screw fitting of

the inline filter cartridge

The flow direction is designated on inline filter cartridges. The inline filter and fitting are inserted into the pressure sensor in such a way that the designating notch always points upwards.

Tools  Torque wrench

Component defect

Damaging the threads of components caused by overtightened fittings. Pay attention to the torque values.

 Use 5 Nm for stainless steel fittings.  Use 1 Nm for PEEK fittings.

Procedure Process Figure

1. Insert the inline filter cartridge

with the designating notch pointing upwards

fitting

2. Manually, screw the fitting with

the inline filter cartridge in the pressure sensor.

3. Using the torque wrench,

tighten the fittings with the appropriate torque.

2 .

1 into the

Fig. 34 Inserting the inline filter car-

tridge into the fitting

Next Steps Reinstall the capillary at the pressure sensor below the fitting of the inline fil-

ter.

P 6.1L user manual V6890

Maintenance and Care 48

Replacing the Mixer

A clogged mixer can cause pressure fluctuations and irregular flow. The mixer is replaced completely as an assembly.

Voraussetzung  The mixer has been rinsed with isopropanol.

 The sealing plugs are on site.

Werkzeuge  Open-end wrench, size 1/4”

 Allen wrench, size 2 mm

Ablauf Abbildung

1. Remove capillary

and outlet of the mixer by hand or with the open-end wrench.

1. Seal the inlet and the outlet with

the sealing plug.

2. Remove the screws

Allen wrench.

3. Detach the mixer and set aside.

4. Install new mixer.

5. Manually, screw the fittings of

the capillary in the mixer.

6. Tighten with a torque wrench

using the appropriate torque.

Nächste Schritte  Bring the device into operation again.

1at the inlet

2 with the

Fig. 35 HPLC Mixer

Fig. 36 Bio Mixer

Removing a Leak

Prerequisites If liquid enters the inside the device, switch off the device. If this is not the

case, it is not necessary to switch off the device.

Auxiliary Material cloth for drying the leak sensor

Procedure Process

1. Remove the leak.

2. Dry the leak tray.

3. Acknowledge the system error via Mobile Control, or the standby but-

ton (only in standalone mode).

Next Steps Afterwards take the device into operation.

P 6.1L user manual V6890

49 Technical Data

Technical Data

P 6.1L General Data

Main Features

Solvent conveyance Variants Isocratic HPLC pump

Delivery system Dual-piston pump

Pulsation compensation Active pressure and pulsation compensation

Pulsation < 2 % Amplitude (typically: < 1,3 %) or

Flow rate range 0,001–10 ml/min

Quaternary low-pressure gradient pump Binary high-pressure gradient pump

< 3 bar (0,3 MPa), whatever is greater, at 1 ml/min ethanol, at all pressures > 10 bar (1 MPa, 147 psi)

0,02–10 ml/min (recommended) 0,01–50 ml/min 0,1–40 ml/min (recommended)

Flow rate increment 0.001 ml/min

Flow rate accuracy < 1 % (measured at 5-80 % of flow range,

Flow rate precision 0.1 % RSD (based on the retention time at

Flushing piston seal Standard

System protection Soft start, P

Wetted materials Stainless steel, carbon-fiber-reinforced PTFE,

Degasser module Degasser channels

Degasser max. flow rate 10 ml/min

Degasser method

Degasser efficiency < 0.5 ppm dissolved O

Degassing chamber volume

using ethanol)

constant room temperature)

und P

min

FKM, PEEK, saphire, aluminum oxide

2O3

)

(Al

2 channels, Teflon

Gas permeation using Teflon phous fluoropolymer membrane

480 µl volume per channel

are programmable

max

AF amor-

at 1 ml/min

eluents Limitations: hydrochloric acid and haloge-

nated hydrocarbons, in particular hexafluoroisopropanol (HFIP)

Wetted materials

Vacuum chamber Polypropylene and stainless steel

Vacuum pump Low hysteresis

P 6.1L user manual V6890

PEEK, Tefzel

, Teflon® AF

Technical Data 50

Communication

Interfaces  LAN

 Pin header connectors (Analog IN, Start

IN, Error IN)

Control  LAN

 Analog and event control  Mobile Control

Analog input 0-10 V

Analog control input Flow rate

Level / event outputs 8 event outputs (TTL, OC, Relais) and 24 V

programming 19 programs, 9 program links, 1 WAKE UP

program

GLP RFID pump head detection, detailed report

display 3 LEDs

leak sensor Yes

protection type IP-20

General

Power supply Power input: 100-240 V

Output: 50-60 Hz Maximum power consumption: 100 Watt

dimensions (W × H × D) 361 mm x 208.2 mm x 523 mm

weight (see ’Device Variants’ auf Seite 50.)

Leak sensor yes

temperature range 4-40 °C (39.2-104 °F)

air humidity below 90 %, non-condensing

Device Variants

P 6.1L isocratic

Setup Pump type Isocratic analytical HPLC pump

Pump Head Versions 5 ml/min stainless steel

10 ml/min stainless steel 50 ml/min stainless steel 10 ml/min ceramic 50 ml/min ceramic

Delay volume 60 µl (at 10 ml/min)

Weight weight 11.5 kg

P 6.1L user manual V6890

51 Technical Data

P 6.1L binary

Setup Pump type Binary analytical HPLC pump with degasser

Pump Head Versions 5 ml/min stainless steel

degasser 2 channels

Solvent selection valve 2 x 2 channels

Weight weight 14.1 kg

Gradient formation Gradient type High-pressure gradient

Gradient range 0–100 %

Minimum increment 0.1 %.

10 ml/min stainless steel 10 ml/min ceramic 10 ml/min for stainless steel

for Normal Phase applications

5–95 % (recommended)

Gradient precision ± 0,3 % (measured at 1 ml/min, 150 bar,

tracer: ethanol/caffeine) ± 1 % (5–95 %, measured at 0.1-10 ml/

min, tracer: water/caffeine)

Gradient repeat accuracy < 0.1 % RSD (measured at 1 ml/min, 0.3 %

RSD overall, based on retention time at constantroom temperature)

Mixer Mixing volume 50, 100, 200 µl;

250 µl (metal-free)

Delay volume 110 l (dependent of mixer),

410 l (metal-free)

P 6.1L quaternary

Setup Pump type Quaternary analytical HPLC pump with

degasser

Pump Head Versions 5 ml/min stainless steel

10 ml/min stainless steel 10 ml/min ceramic

degasser

Special feature Automatic adaption of LPG cycle time

Weight weight 12.7 kg

Gradient formation Gradient type Low-pressure gradient

Gradient range 0–100 %

Minimum increment 0.1 %.

P 6.1L user manual V6890

4 channels, Teflon

1-99 % (recommended)

Repeat Orders 52

Gradient precision ± 0,3 % (measured at 1 ml/min, 150 bar,

tracer: ethanol/caffeine) ± 2 % (1-99 %, measured at 5-50% of the flow range, tracer: water/caffeine)

Gradient repeat accuracy < 0.1 % RSD (measured at 1 ml/min, 0.5 %

RSD overall, based on retention time at constantroom temperature)

Mixer Mixing volume 50, 100, 200 µl;

250 µl (metal-free)

Delay volume 210 l (dependent of mixer),

410 l (metal-free)

Pump Heads

Flow rate range 5 ml pump head 0.001 ml/min-2 ml/min

10 ml pump head 0.001 ml/min-10 ml/min

0.02-10 ml/min (recommended).

Maximum pressure 5 ml pump head  1000 bar (100 MPa, 14504 psi) up to

Repeat Orders

Further Information Further information on spare parts and accessories can be found online:

50 ml pump head 0.001 ml/min-50 ml/min

0.01-40 ml/min (recommended).

2 ml/min

 700 bar (70 MPa, 10150 psi)

10 ml pump head stainless steel

 700 bar (70 MPa, 10150 psi) up to 5 ml/

min

 400 bar (40 MPa, 5800 psi)

Ceramic

 400 bar (40 MPa, 5800 psi)

50 ml pump head 200 bar (20 MPa, 2900 psi)

This list for repeat orders is valid for the time the document has been published. Deviations afterwards are possible. Note: For repeat orders of spare parts use the enclosed packing list. Contact

the Technical Support in case there are any questions on spare parts or accessories.

www.knauer.net

Devices

Name Order num-

ber

P 6.1L isocratic with 10 ml stainless steel pump head APH30EA

P 6.1L isocratic with 10 ml stainless steel pump head for Normal Phase

P 6.1L isocratic with 50 ml stainless steel pump head APH30FA

P 6.1L user manual V6890

APH30ED

53 Repeat Orders

Name Order num-

ber

P 6.1L isocratic with 50 ml stainless steel pump head for Normal Phase

P 6.1L isocratic with 10 ml ceramic pump head APH60EB

P 6.1L isocratic with 50 ml ceramic pump head APH60FB

P 6.1L binary with 10 ml stainless steel pump head, degasser and mixer (100 µl)

P 6.1L binary with 10 ml stainless steel pump head for Normal Phase, degasser and mixer (100 µl)

P 6.1L binary with 5 ml stainless steel pump head and mixer (100 µl)

P 6.1L binary with 10 ml stainless steel pump head and mixer (100 µl)

P 6.1L binary with 10 ml stainless steel pump head for Normal Phase and mixer (100 µl)

P 6.1L binary with 50 ml stainless steel pump head and mixer (200 µl)

P 6.1L binary with 10 ml ceramic pump head, degasser and mixer (250 µl)

APH30FD

APH35EA

APH35ED

APH35GA

APH38EA

APH38ED

APH38FA

APH65EB

P 6.1L binary with 10 ml ceramic pump head, degasser and mixer (250 µl)

P 6.1L binary with 50 ml ceramic pump head, degasser and mixer (250 µl)

P 6.1L quaternary with 10 ml stainless steel pump head, degasser and mixer (100 µl)

P 6.1L quaternary with 5 ml stainless steel pump head, degasser and mixer (100 µl)

P 6.1L quaternary with 10 ml stainless steel pump head and mixer (100 µl)

P 6.1L quaternary with 10 ml ceramic pump head, degasser and mixer (250 µl)

P 6.1L quaternary with 50 ml ceramic pump head, degasser and mixer (250 µl)

APH68EB

APH68FB

APH34EA

APH34GA

APH39EA

APH64EB

APH69EB

Accessories and Spare Parts

Name Order num-

ber

Pump head Pump head, 5 ml, stainless steel AHA60

Pump head, 10 ml, stainless steel AHB40

Pump head, 10 ml, ceramic AHB32

Pump head 10 ml, stainless steel for Normal Phase AHB40BA

P 6.1L user manual V6890

Repeat Orders 54

Name Order num-

ber

Pump head, 50 ml, stainless steel AHC20

Pump head 50 ml, stainless steel for Normal Phase AHC20BA

Pump head, 50 ml, ceramic AHC22

Check valves Check valve unit for AZURA 5, 10 ml pump head, ruby

G0924B

sapphire

Check valve unit for AZURA 5, 10 ml pump head, nor-

G0563-5

mal phase, spring-supported, ruby sapphire

Check valve unit for AZURA 50 ml pump head, ruby

G0924C

sapphire

Inline filter cartridge Filter cartridge, stainless steel, 2 m (20 l volume) A96601

Filter cartridge, titanium, 2 m (60 l volume) A9661

Mixer AZURA mixer 50 l AZZ00MB

AZURA mixer 100 µl AZZ00MC

AZURA mixer 200 µl AZZ00MD

AZURA mixer 250 µl, bio AZZ10ME

Solvent tray AZURA solvent tray E 2.1L AZC00

Drainage System corrugated hose, 16 cm, PE grey A9846-1

corrugated hose, 150 cm, PE grey A9846-3

funnel P6431

exhaust P6432

Capillary guide capillary guide top P6424

Capillary guide side P6425

Mobile Control Mobile Control license with 10” touchscreen A9607

Mobile Control Chrom license with 10” touchscreen A9608

Mobile Control license A9610

Mobile Control Chrom license A9612

Accessories Kit AZURA accessories kit FZA02

Accessories kit P 6.1L isocratic FPH30

Accessories kit P 6.1L quaternary FPH34

Accessories kit P 6.1L binary FPH35

Tools AZURA tool kit A1033

Capillary cleaning kit A0137

Metal capillary cutter A0681

Product riser AZURA product riser (28 mm) A9860

P 6.1L user manual V6890

55 Legal Information

Name Order num-

Power cable USA M1651

UK M1278

Switzerland M1597

Europe M1642

Documents User Manual Pump P 6.1L V6890

Installation Qualification Document VIQ_INST

Operation Qualification Document VOQ_PUMPS

Legal Information

Transport Damage

The packaging of our devices provides the best possible protection against transport damage. Check the devices for signs of transport damage. In case you notice damages, contact the Technical Support and the forwarder company within three workdays.

ber

Warranty Conditions

The factory warranty for the device is stipulated by contract. During the warranty period, any components with material or design-related defects will be replaced or repaired by the manufacturer free of charge. Please connect to our website for further information on terms and conditions.

All warranty claims shall expire in the event that any unauthorized changes are made to the device. This warranty also excludes the following:

 accidental or willful damage  damage or errors caused by third parties that are not contractually

related to the manufacturer at the time the damage occurs

 wear parts, fuses, glass parts, columns, light sources, cuvettes and other

optical components

 damage caused by negligence or improper operation of the device and

damage caused by clogged capillary

 packaging and transport damage

In the event of device malfunctions, directly contact the manufacturer. KNAUER Wissenschaftliche Geräte GmbH

Hegauer Weg 38 14163 Berlin, Germany Phone: +49 30 809727-111 Telefax: +49 30 8015010 e-mail: info@knauer.net Internet: www.knauer.net

Declaration of Conformity

The Declaration of Conformity is part of the delivery and accompanies the product as a separate document.

P 6.1L user manual V6890

HPLC Glossary 56

Warranty Seal

The warranty seal is color-coded. A blue seal is used by the assembly or technical support of KNAUER for devices to be sold. After repair, service technicians affix an orange seal in identical position. If unauthorized persons interfere with the device or the seal is damaged, the warranty claim will forfeit.

Disposal

Hand in old devices or disassembled old components at a certified waste facility, where they will be disposed of properly.

AVV Marking in Germany According to the German "Abfallverzeichnisverordnung" (AVV) (January,

2001), old devices manufactured by KNAUER are marked as waste electrical and electronic equipment: 160214.

WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in

the German "Elektroaltgeräteregister" (EAR). The number belongs to category 8 and 9, which, among others, comprise laboratory equipment.

All distributors and importers are responsible for the disposal of old devices, as defined by the WEEE directive. End-users can send their old devices manufactured by KNAUER back to the distributor, the importer, or the company free of charge, but would be charged for the disposal.

Solvents and Other Operat-

ing Materials

All solvents and other operating materials must be collected separately and disposed of properly.

All wetted components of a device, e. g. flow cells of detectors or pump heads and pressure sensors for pumps, have to be flushed first with isopropanol and then with water before being maintained, disassembled or disposed.

HPLC Glossary

Here you find definitions for abbreviations and technical terms, which are used in liquid chromatography.

Term Definition

Binary pump Pump configured to generate a gradient from two solvents.

Chromatogram Record of a detector signal, depending on output volume of mobile

phase and time

Degasser Degasser module for fluids

Detector device measuring the composition or the quantity of a substance.

Pressure sensor Component for measuring the system pressure

Eluent mobile phase transporting substances to be separated or isolated through

the column

Flangeless fitting Fitting without a protruding edge, which can be fastened manually.

GLP Good Laboratory Practice, quality assurance system for laboratories

P 6.1L user manual V6890

57 HPLC Glossary

Term Definition

Gradient Time-dependent composition of solvent (mobile phase) on low-pressure

or high-pressure side of system

LPG pump Pump configured to generate a gradient on the high-pressure side. Usu-

ally a binary pump with two separate pump units which each convey one solvent. The gradient is generated behind the pump.

High-pressure side Devices and capillary which are under high pressure in an HPLC system

HPLC High-Pressure Liquid Chromatography (HPLC) High pressure liquid chro-

matography

Isocratic Mode of sample separation where the composition of a solvent remains

constant

Capillary thin metal or PEEK pipe that connects components and devices within the

chromatography system

Check valve Valve unit built into the pump head which guides the flow of liquid from

the inlet to the outlet.

LAN Local area network (LAN). Network in which HPLC devices and a com-

puter are connected, in order to control the devices.

LED Light-emitting diode

Solvent mobile solvent transporting substances to be separated or isolated

through the column

Ground Protective measure for electroconductive housing parts

Mixer Component for the homogenous mixing of solvents for the generation of

a gradient

Mobile Control Mobile application to control and program the whole HPLC system via

wireless LAN.

NPG pump Pump configured to generate a gradient on the low-pressure side. Only

one pump unit is used which contains small doses of the different solvents. The solvents are mixed in small mixing cycles by a valve block which is connected upstream. The gradient is generated behind the pump.

Low-pressure side Devices and capillary which are under high pressure in an HPLC system

pH Measure for the acidic or alkaline character of an aqueous solution.

Pump Device which conveys solvents in controlled quantities into a system.

Quaternary pump Pump configured to generate a gradient from four solvents.

Column The column is filled with a matrix which separates the single components

of a substance spatially by interacting with an analyte transported with the solvent to different degrees.

Solvent selection valve Component of the pump that allows selecting from two different solvents

for every solvent channel. With this mechanism the solvent can be changed without having to manually change the system.

Dead volume Volume of capillaries and system components between mixing chamber,

injector and column as well as between column and detector.

Valve device to diversify the eluent flow

P 6.1L user manual V6890

Index

Index 58

additives 9 ambient conditions 14, 43 Analog port 30 AVV marking 56

Binary pump 6, 7, 16, 17

Cap fitting 17, 18, 20 capillary 57

fitting 15 pre-installed 15

Check valve 45

cleaning 45 installing 45

removing 45 chromatogram 56 clean 41 Computer

control contamination 12 Control 23

dead volume 57 decontamination 12 Degasser

2-channel

4-channel 7, 20

degassing chamber 20

Eluent list 10 dimensions 50 disposal 12

Electrical connections

Remote terminal strip eluents 9 error 33

Fitting

flangeless fitting 15 Fittings

checking FPLC 8 front cover 15

15, 56

GLP data 9

High-pressure side 57 HPLC 8

Inline Filter

Removing

Inline filter cartridge

inserting. reorder 54

installation

site

IQ 34 Isocratic pump 6, 16

label 15 LAN 23

port 25 problems 35 router 24 settings 23 setup 24

troubleshooting 35 leak 12 leak management 22 leak sensor 22 LED 33 Low-pressure side 57

maintenance

contract

intervals 40 Mixer

bio-inert

replacing 48 Mixing chamber

reorder mixing chamber 8 modifiers 9

operating environment 14 Operating Hours 40 operating ranges 6 OQ 34

packing list 15 pin header 26 Piston backflushing 9, 44

Connecting 21

P 6.1L user manual V6890

59 Index

PMax Mode 9 port (LAN) 25 power

disconnect

plug 14 power cable 12 power strip 12 power supply 12

connect 30 Pressure sensor 6, 7 professional group 11 Pump

bio-inert

bleeding 31

Flushing 41

Integrating into System 16 Pump head

bio-inert

connecting 20

dismounting 43

storing 43 pump head

reorder Putting the Pump Out of Operation 42

Quarternary pump 7, 16 Quaternary Pump 19 Quaternary pump 6, 57

remote connector

pin header repair 12, 13 RFID chip 8 router (LAN) 24

safety equipment 11 salts 9 scope of delivery 15

Screw fittings

torque

Service Interface 8 software 8, 22 solvent

flammability line 12 self-ignition point 12 tray 12

Solvent bottles

connecting Solvent selection valve 18 space requirements 14 standby 33

malfunction 33 switch on 32

Technical Data

gradient information binary

gradient information quaternary 51 technical data

general data

pump head 52

solvent conveyance 49 test

Installation Qualification

Operation Qualification 34 Torque 46, 47 transport damage 55 Troubleshooting

First Measures troubleshooting

LAN

Valve block 21 Venting screw 7

warranty 55

P 6.1L user manual V6890

Latest manuals online:

www.knauer.net/downloads

HPLC · SMB · Osmometry

KNAUER Wissenschaftliche Geräte GmbH Hegauer Weg 38 14163 Berlin

Phone: +49 30 809727-0 Fax: +49 30 8015010 e-Mail: info@knauer.net Internet: www.knauer.net

www.knauer.net

Knauer Azura P 6.1L User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Table of Contents

Product Information

Operating Ranges

Device Variants

Views

Front View

Rear View

Performance Features

Eluents

Scope of Delivery

Safety for Users

Signal Words

Decontamination

Symbols and Signs

Installation

Transport

Location Requirements

Unpacking the Device

Capillaries and Fittings

Connecting the Solvent Bottles

Pre-Installed Capillaries

Integrating the Pump into an HPLC Flow System

Isocratic pump

Connecting the Pump Head to the Solvent

Binary pump

Connecting the Degasser

Connecting the Pump Head to the Solvent

Connecting the Solvent Selection Valve to the Solvent

Quaternary Pump

Connecting the Degasser

Connecting the Pump Head to the Solvent

Connecting the 4-Channel Degasser to the Solvent

Connecting the Valve Block

Connecting the Piston Backflushing

Connecting the Leak Management

Control

Computer Control

Configuring the LAN Settings

Connecting the Cables

Configuring the Router

Integrating the LAN into a Company Network

Controlling Several Systems Separately in a LAN

Setting a Static IP Address

Pin Header Connectors

Remote Terminal Strip

Events Connector

Connecting Cables to the Terminal Strip

Analog Control

Power Connection

Initial Start-Up

Start-Up

Pump Head

Bleeding the Pump

Switch-On

Operation

Operating with Chromatography Software

Operating with Mobile Control

Meaning of the LEDs

Functional Tests IQ and OQ

Troubleshooting

Possible Problems and Solutions

System Messages

Maintenance and Care

Maintenance Contract

Maintenance Intervals

Cleaning and Caring for the Device

Checking the Fittings

Flushing the Pump

Putting the Pump Out of Operation

Storage

Disconnecting the Power Supply

Preparing the Pump Head for Storage

Dismounting the Pump Head

Check Valves

Removing the Check Valves

Cleaning the Check Valve