On the front of the device, you find the flow cell and a lamp
cover for replacing the lamp.
Fig. 1Front view (example DAD 6.1L)
Rear View
On the back of the detector, the connectors for LAN and power
plug are located as well as the serial number of the device and
the power switch (on/off).
RS-232, service interface Please note that the service interface is solely used for repair and
maintenance tasks performed by service technicians.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Legend
2345
6
71
1
1 Device information
2 Integrator outlets
3 LAN connector
4 Service Interface
5 Pin header
6 Fan
7 Connection and
power switch
7Intended Use
Fig. 2Rear view (example DAD 6.1L)
Side View
DAD 6.1LOn the side of the detector, you find the connector for the oper-
Legend
1 USB port for the
Control Unit
ating panel Control Unit (see See “Control with Mobile Control”
on page 32.).
Note The USB ports of the DAD 2.1L and the MWD 2.1L are not
active. These detectors cannot be operated with the Control
Unit.
Fig. 3USB port for the Control Unit
Features
In an HPLC system, the detector serves to detect substances in
liquids and show their concentration. The sensitivity of the
detector depends on the installed flow cell.
Optical systemThe detectors measure the light absorption of the sample in the
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
ultraviolet and visual spectra. The entire spectrum permeates the
sample and afterwards is split up by a grate. The split light falls
onto a geometric array of 1024 (DAD 6.1L) or 256 (DAD 2.1L,
MWD 2.1L) separate photodiodes – the photodiode array.
Legend
3
5
1
2
4
6
1 Deuterium lamp
2 Halogen lamp
3 Flow cell
4 Diode array sensor
5 Diffraction grating
6 Mirror
8Intended Use
The optical system of the AZURA DAD 6.1L detector is depicted
in figure 4. An active temperature control with 0.5°C sensitivity
ensures baseline stability for minimal baseline drift.
Fig. 4Optical system DAD 6.1L
LampsThe lamps can be exchanged quickly and easily without the
need of a service technician.
DAD 6.1LTwo light sources, a deuterium lamp and a halogen lamp, cover
the entire UV-Vis wavelength range of remove space
190–1020 nm.
DAD 2.1L, MWD 2.1LA deuterium lamp covers the UV wavelength range of 190–500
nm.
Lamp mirror
DAD 6.1L
Flow cellVarious types of flow cells are available to the user and allow a
KNAUER LightGuide
flow cell cartridge
Data rateThe detector measures with a maximum data rate of 100 Hz.
Maximum light intensity is provided by effectively mixing the
light sources from the deuterium and halogen lamps with a mirror using Polka Dot technology.
variety of applications. Flow cell cartridges with standard and
KNAUER LightGuide technology are available.
KNAUER LightGuide flow cell cartridges combine a maximum
light transmission (using total reflection) with a minimum cell
volume to offer an ideal signal-to-noise ratio.
Adapter for fiber optics are additionally available. Fiber optics
allow separating the flow cell from the detector. This way, working with dangerous, explosive, or toxic materials becomes safer.
During the measurement, approx. 100 spectra per second are
recorded.
Leak ManagementIntegrated leak management drains liquids resulting from leaks.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
9Intended Use
GLP dataThe Mobile Control and the different software products (e.g.
ClarityChrom
®
or OpenLAB®) can be used to display or read out
the GLP data (e.g. operating hours, lamp operating hours, number of lamp ignitions, etc.) of the detector. For the DAD 6.1L,
the Control Unit is available for reading out the GLP data. You
find a detailed description on how to display or read out GLP
data in the respective user manuals.
Optional accessoriesThe following optional accessories are available:
The Mobile Control app for control on Android and
Windows 8
The Control Unit for operating the DAD 6.1L
Solvents
Even small quantities of other substances, such as additives,
modifiers, or salts can influence the durability of the materials.
If there is any doubt, contact the Technical Support of the manufacturer.
Suitable solvents
Acetate buffer solutions
Acetone at 4 °C–25 °C (39.2 °F–77.0 °F)
Acetonitrile
Perfluorinated solvents, e.g. Fluorinert
Perfluorinated polyether, e.g. Fomblin
®
FC-75, FC-40
®
Solvents containing particles
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
11Scope of Delivery
Scope of Delivery
Note Only use spare parts and accessories made by KNAUER or a
company authorized by KNAUER.
DAD 6.1LDetector
Power cable
User manual English/German
Installation Qualification documents
Accessory kitDAD 6.1L
AZURA
DAD 2.1L Detector
Power cable
User manual English/German
Installation Qualification documents
Accessory kitDAD 2.1L
AZURA
MWD 2.1LDetector
Power cable
User manual English/German
Installation Qualification documents
Accessory kitMWD 2.1L
AZURA
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
12Safety for Users
Safety for Users
Professional groupThe user manual addresses persons who are qualified as chemi-
cal laboratory technicians or have completed comparable vocational training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by
If you do not belong to this or a comparable professional group,
you may not perform the work described in this user manual
under any circumstances. In this case, please contact your superior.
Safety equipmentWhen working with the device, take measures according to lab
regulations and wear protective clothing:
limited extent for use in liquid chromatography
the company KNAUER or an authorized company.
What must be taken into
account?
Safety glasses with side protection
Protective gloves
Lab coat
All safety instructions in the user manual
The environmental, installation, and connection specifica-
tions in the user manual
National and international regulations pertaining to labora-
tory work
Original spare parts, tools, and solvents made or recom-
mended by KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident
insurance companies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once they have been used, never re-use capillaries in other
areas of the HPLC system.
Only use a given PEEK fitting for one specific port and never
re-use it for other ports. Always install new PEEK fittings on
each separate port.
Follow KNAUER or manufacturer's instructions on caring for
the columns
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
13Safety for Users
More safety-relevant information is listed in alphabetical order in
the following table:
TopicExplanations
FlammabilityOrganic solvents are highly flammable.
Since capillaries can detach from their
screw fittings and allow solvent to escape,
it is prohibited to have any open flames
near the analytical system.
Solvent trayRisk of electrical shock or short circuit if
liquids get into the device's interior. For
this reason, place all bottles in a solvent
tray.
Solvent linesInstall capillaries and tubing in such a way
that liquids cannot get into the interior in
case of a leak.
LeaksRegularly check if any system compo-
nents are leaking.
Where is use of the
device prohibited?
Power cableDefective power cables are not to be used
to connect the device and the power supply system.
Self-ignition
point
Only use eluents that have a self-ignition
point higher than 150 °C under normal
ambient conditions.
Power stripIf several devices are connected to one
power strip, always consider the maximum power consumption of each device.
Power supplyOnly connect devices to voltage sources,
whose voltage equals the device's voltage.
ToxicityOrganic eluents are toxic above a certain
concentration. Ensure that work areas are
always well-ventilated! Wear protective
gloves and safety glasses when working
on the device!
Never use the system in potentially explosive atmospheres without appropriate protective equipment. For further information,
contact the Technical Support of KNAUER.
Taking the device out of
operation
At any time, take the device completely out of operation by
either switching off the power switch or by pulling the power
plug.
Opening the deviceThe device may be opened by the KNAUER Technical Support or
any company authorized by KNAUER only.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
14Safety for Users
Definition of Personal and Material
Damage
Possible dangers related to the device are divided into personal
and material damage in this user manual.
CategoryExplanations
DANGER!Lethal or very serious injuries can occur.
WARNING!Serious or moderate injuries can occur.
CAUTION!Moderate injuries can occur. Device defects
can occur.
Decontamination
Contamination of devices with toxic, infectious or radioactive
substances poses a hazard for all persons during operation,
repair, sale, and disposal of a device.
DANGER!
Danger if getting in contact with toxic, infectious
or radio-active substances.
Before disposing off or sending away contaminated devices, commission an expert with the
decontamination.
All contaminated devices must be properly decontaminated by a
specialist company or the operating company before they can
be recommissioned, repaired, sold, or disposed of.
All materials or fluids used for decontamination must be collected separately and disposed of properly.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
15Symbols and Signs
Symbols and Signs
The following symbols and signs can be found on the device, in
the chromatography software or in the user manual:
SymbolMeaning
Warning signsHigh-voltage hazard
Electric shock hazard
Electrostatic discharge hazard, damages
to system, device, or components may
occur.
General warning sign, moderate injuries
may occur and also damages to system,
device, or components.
UV-light hazard, eye injuries may occur.
Burn hazard, hot surface
Hazardous substances.
Obey maximum load for leak tray during
transportation, installation and operation.
Mandatory signsWear protective gloves to avoid skin irrita-
tions.
CE markA device or system marked with CE fulfills
the product specific requirements of European directives. This is confirmed in a
Declaration of Conformity.
Testing seals in Canada and the USA at
nationally recognized testing centers
(NRTL). The certified device or system has
successfully passed the quality and security tests.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
16Installation
Installation
This chapter describes all preparatory steps prior to the start-up.
If you encounter difficulties during installation, contact the
Technical Support.
Contact Data
Technical SupportPhone+49 30 809727-111
Fax+49 30 8015010
E-mailsupport@knauer.net
Transport
Carefully prepare the device for transport or storage. If you want
to return your device to KNAUER for repairs, enclose the Service
Request Form which can be downloaded from our website.
Device dataFor a secure transport, note the weight and dimensions of the
device (see Technical Data).
CAUTION!
Damage to the device by lifting on protruding
housing parts!
Lift the device on the side of the housing only.
LifitingClasp the device at its side panels and lift it out of the packag-
ing. Do not hold onto front cover or leak tray.
Only if the requirements for ambient conditions of the operating
environment are met, can the intended use be ensured. You will
find the ambient conditions under Technical Data.
CAUTION!
Defect of the device due to overheating possible!
Protect the device against exposure to direct sunlight.
Keep clear at least 15 cm at the rear and 5–10 cm
at each side for air circulation.
Installation Site and Space Requirements
Position the device on a level and even surface.
Sunlight: Protect the device against direct exposure to sun-
light.
AC system: Set up the device at a location not exposed to air
drafts.
Vibration: Do not set up the device in the vicinity of other
machines that cause floor vibrations.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
17Installation
1
Lateral distance to other devices:
At least 5 cm if another device is set up on one side
At least 10 cm if further devices are set up on both sides
Leave the power plug on the rear of the device accessible
to be able to disconnect the device from the mains.
Avoid sources of high frequencies near the device. High-fre-
quency sources may compromise measuring values.
Operating Environment
Earth quake regionsIf the device is set up in a region where earth quakes are com-
mon, secure the device at the two fixing points
points are situated on both sides of the device.
1 . The fixing
Fig. 5Fixing points on the device
Space Requirements
Side clearance to other devices:
At least 5 cm, if there is another device on one side.
At least 10 cm, if there are devices set up on both sides.
At least 15 cm on the rear panel for the fan.
Setup
Prior to setting up the device, find a suitable place according to
the requirements and remove the packaging. The requirements
and a description can be found in the following section.
Unpacking
PrerequisiteCheck packaging for damage caused during transportation.
ToolUtility knife
CAUTION!
Damage to the device by lifting on protruding
housing parts!
Lift the device on the side of the housing only.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
18Installation
Process
1. Set-up the package in such a way that you can read the
label. Using the utility knife, cut the adhesive tape and
open the packaging.
2. Remove the foam insert. Take out the accessory kit and the
manual.
3. Open the accessory kit and check the scope of delivery. In
case any parts are missing, contact the Technical Support.
4. Clasp the device from below, lift it out of the packaging
and place it on its feet. Do not hold onto the front cover.
5. Check the device for signs of damage that occurred during
transport. In case you notice any damage, contact the
Technical Support.
6. Place the device in its site of operation and remove protective foil.
Next stepsStore packaging and keep the included packing list for repeat
orders.
Note The test cell is used during operation qualifications and must be
stored.
Inserting the Flow Cell
Prerequisite No flow cell or test cell is installed.
The device is switched off.
Procedure Insert the flow cell.
Note Pay attention to the compatibility of the flow cells.
WARNING!
Irritation of retina through UV light! Concentrated UV light can leak out from the flow cell or
the fiber optic connectors.
Switch off the device or the lamps.
ProcedureProcessFigure
1. Place the flow cell into
the opening and slide
to the back until it
clicks into place inside
the device.
Fig. 6Inserting the Flow Cell
Next stepsConnect the capillaries.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
19Installation
1
1
1
3
Inserting the Flow Cell with Fiber Optics
You may also connect the flow cell with fiber optic cables. The
fiber optics are connected via an adapter.
UV light will cause the fiber optics to become blind with time
(solarization), making them no longer suitable for use.
Observe the following regarding the use of UV fiber optics:
Do not touch the ends of the fiber optics with your fingers, as
this could falsify the measurement.
Handle the fiber optics with care, avoid impacts or hard
actions.
Move the fiber optics carefully without using pressure or
bending it.
PrerequisitesNo flow cell or test cell is installed.
Auxiliary materialScrew-type cap fittings for the fiber optics
WARNING!
Irritation of retina through UV light! Concentrated UV light can leak out from the flow cell or
the fiber optic connectors.
Switch off the device or the lamps.
ProcedureProcessFigure
1. Remove the cap fittings
1 from the fiber optics
adapter.
2. Place the fiber optics
adapter into the opening and slide to the
back until it clicks into
place inside the device.
3. Remove the screw-type
cap fittings
fiber optics.
4. Manually, screw the
fiber optics
fiber optics adapter.
5. Remove the cap fittings
from the flow cell.
6. Manually, screw the fittings
3 of the fiber
optics to the flow cell.
1 from the
2 to the
Fig. 7Fiber optics adapter
Fig. 8Screw-type cap fitting
on the fiber optics
Fig. 9Fitting of the fiber
optics on the flow cell
Next stepsConnect the capillaries.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
20Installation
132
4
13
2
4
4
4
Connecting the Capillaries
Capillaries connect the detector with the column and guides liquids. For the flow cell connectors, we recommend PEEK capillaries and PEEK connectors.
CAUTION!
Damage to the PEEK threads of the flow cell
through metal fittings possible.
Attach capillaries with PEEK fittings only.
Observe torque of 0.5 Nm maximum.
Prerequisite The flow cell is inserted.
Screw-type cap fittings and cap fittings are removed.
ProcedureAttach the capillaries with screw fitting.
ProcedureProcessFigure
1. Push the capillary
through the fitting
2. Slide the lock ring
over the tubing. Note
that the tapered end of
the lock ring has to
point to the seal
ring
4.
3. Attach the seal ring
4. Fasten the capillary at
the flow cell
tight.
Next stepsBring the device into operation.
1
2.
3
4.
4 finger-
Fig. 10Capillary connectors
Fig. 11Flow cell with capillary
Using PEEK Fittings
PEEK fittings withstand a maximum pressure of 400 bar. Note
the torque of 0.5 Nm.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
21Installation
1
2
3
4
5
Connecting the Leak Management
The leak management consists of the leak sensor and the drainage system. The drainage system ensures that escaping liquids
flow into a waste bottle. If there is too much liquid, the red LED
starts flashing. Both the device and the data acquisition via chromatography software are stopped.
Prerequisite The front cover has been removed.
Procedure
ProcessFigure
1. Carefully push the fun-
1 into the center
nel
opening of the capillary
guide 2.
Fig. 12Funnel and capillary
2. Push the long ending of
the first nozzle
the hose
3. Connect the nozzle and
the funnel.
4. Push the other end of
the hose onto the noz-
zle
5 of the leak tray.
4 into
3.
Fig. 13Hose and nozzle
guide
Fig. 14Hose connected to
device
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
22Installation
6
1423
ProcedureProcessFigure
5. For the bottom device,
push the short end of
the nozzle
opening in the collection point of the leak
tray.
6. Connect the hose to
the nozzle and lead the
second ending to the
waste bottle.
7. Place the waste bottle
below the bottom
device.
Next stepsAttach the front cover.
6 into the
Fig. 15Leak tray with nozzle
Legend
1 Integrator outlets
2 LAN connector
3 Service interface
4 Pin header
Control
The detector can be controlled externally in two ways:
Via pin header
As part of a LAN, via the LAN connector of the router
All connectors for external control are located on the rear side of
the detector
The DAD 6.1L can also be controlled via the Control Unit. The
Control Unit connector is located on the side of the device.
.
Fig. 16Connectors on rear side
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
DAD 6.1L
5
1 USB port for the
Control Unit
23Installation
Fig. 17Control Unit port on DAD 6.1L
Connecting the Device to the Computer
This section describes how to set up an HPLC system in a local
area network (LAN) and how a network administrator can integrate this LAN into your company network. The description
applies to the operating system Windows® and all conventional
routers.
Note To set up a LAN, we recommend to use a router. That means
the following steps are required:
Process1. On the computer, go to the control panel and check the LAN
properties.
2. Hook up the router to the devices and the computer.
3. On the computer, configure the router to set up the network.
4. Install the chromatography software from the data storage
device.
5. Switch on the device and run the chromatography software.
Configuring the LAN Settings
The LAN uses only one server (which is normally the router)
from that the devices automatically receive their IP address.
Prerequisite In Windows
screen saver must be deactived.
In case you use an USB-to-COM box, the option "Allow the
computer to turn off ths device to save power" in the devicemanager must be deactivated for all USB hosts.
Only for Windows 7: For the network adapter, the option
"Allow the computer to turn off this device to save power" in
the Device Manager must be deactivated.
®
, power saving, hibernation, standby, and
Procedure1. In Windows 7 choose Start
Sharing Center.
2. Double-click on LAN Connection.
3. Click on the button Properties.
4. Select Internet Protocol version 4 (TCP/IPv4).
5. Click on the button Properties.
6. Check the settings in the tab General. The correct settings for
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
the DHCP client are:
Control Panel Network and
24Installation
12
3
4
5
a) Obtain IP address automatically
b) Obtain DNS server address automatically
7. Click on the button OK.
Connecting the Cables
A router has several LAN ports and one WAN port that can be
used to integrate the LAN into a wide area network (WAN), e.g.
a company network or the Internet. In contrast, the LAN ports
serve to set up a network from devices and a computer. To
avoid interference, we recommend operating the HPLC system
separately from the company network.
You will find patch cables for each device and the router in the
accessories kit. To connect the router to a WAN, an additional
patch cable is required, which is not supplied within the scope
of delivery.
Legend
1 Modules
2 Router
4 WAN port
5 Workstation
3 LAN ports
Fig. 18Cabling system LAN
Prerequisite The computer has been switched off.
There is a patch cable for each device and the computer.
Procedure1. Use the patch cable to connect the router and the computer.
Repeat this step to connect all devices.
2. Use the power supply to connect the router to the mains
power system.
Configuring the Router
The router is preset at the factory. You will find a label at the
bottom side of the router, on which IP address, user name, and
password are printed. These information help to open the router
configuration.
Procedure1. To open the router configuration, start your Internet browser
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
and enter the IP address (not for all routers).
2. Enter user name and password.
25Installation
3. Configure the router as DHCP server.
4. In the router configuration, check the IP address range and
make changes if necessary.
ResultOnce the router has assigned IP addresses to all devices, the
chromatography software can be used to remotely control the
system.
Integrating the LAN into a Company Network
A network administrator can integrate the LAN into your company network. In this case you use the WAN port of the router.
PrerequisiteThere is a patch cable for the connection.
Procedure1. Check that the IP address range of the router and of the com-
pany network do not overlap.
2. In case of an overlap, change the IP address range of the
router.
3. Use the patch cable to connect the router WAN port to the
company network.
4. Restart all devices, including the computer.
Controlling Several Systems Separately in a LAN
Devices connected to a LAN communicate through ports, which
are part of the IP address. If more than one HPLC system is connected to the same LAN and you plan on controlling them separately, you can use different ports to avoid interference.
Therefore, the port number for each device must be changed
and this same number must be entered into the device configuration of the chromatography software. We recommend to use
the same port number for all devices in the same system.
Note The port is set to 10001 at the factory. You must use the same
numbers in the device configuration of the chromatography
software as in the device, otherwise the connection fails.
Procedure1. Find out port number and change it on the device.
2. Enter the port number in the chromatography software.
ResultThe connection is established.
Pin Header Connectors
For test purposes or in some other cases, it can make sense to
manually enter these signals.
Sending control signals (Events) to external devices
Opening and closing contacts
Activating 500 ms pulses
The following remote signals can be received and sent:
For receiving start, control, and error signals from external
devices
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
26Installation
For sending start, control and error signals to external devices
Fig. 19Relation between display and Events terminal strip
Explanations for Pin Header Connector
ConnectionFunction
Relay contact
EV 1
(Event 1)
The contact is on a floating basis. Its
setting depends on the settings in the
Control Unit, the Mobile Control, or
software.
Steady-rate signal:
passive = open relay contact
active = closed relay contact
Pulse:
Closed relay contact for at least
1000 ms
Permissible load of the relay contact:
1 A/ 24 V DC
EV 2 (Event 2)TTL output
Levels:
passive 5 V
active 0 V
Pulse:
0 V for at least 1000 ms
Error OUTTTL output
Levels:
passive 5 V
active 0 V
Output is active until the Error condition has been eliminated.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
27Installation
ConnectionFunction
Error INTTL input
Start INTTL input
Low active
Secure switching threshold at least
10 mA
After receiving a signal (short-circuit to
ground) from an external device, an
error message appears and the device
stops.
Low active
Secure switching threshold at least
10 mA
After receiving a signal (short-circuit to
ground) from an external device, the
device starts. If controlled with software, an electronic trigger is send
through the LAN.
AutozeroTTL input
Low active
Secure switching threshold at least
10 mA
A signal (short-circuit to ground) sets
the measuring signal to zero.
+5 VProvides a voltage of 5 V with respect
to GND. This makes it possible to supply a load that is switched by an
EVENT.
Protection: 5 V-50 mA
GNDReference point of the voltage at the
signal inputs.
+24 V ValveEvent-controlled switching of 24 V
against GND
Protection: 24 V-200 mA
Valve OUTOutput is active until the valve condi-
tion has been eliminated.
GNDReference point of the voltage at the
signal inputs.
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28Installation
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2
3
Wiring the Pin Header
To control one device through another, you use the pin header.
The single ports are used to exchange control signals.
To use remote control, you have to connect cables to the pin
header. Therefore, connect the cables firstly to the spring strip
and push the spring contacts of the spring strip secondly onto
the pins of the pin header.
Prerequisites The device has been turned off.
The power plug has been pulled.
ToolsDepressor tool
CAUTION!
Short-circuit hazard.
Turn off the device before connecting it to the
pin header. Pull the power plug.
CAUTION!
Electrostatic discharge can destroy the electronics!
Wear protective bracelet against electrostatic discharge and ground.
ProcedureProcessFigure
1. Place the spring strip
on a suitable surface.
2. Push the depressor tool
into the opening on the
spring strip.
3. Continue pushing the
depressor tool down and
lead the cable
front end of the spring
strip.
4. Remove the depressor
tool.
5. Check whether the cables
are tightly attached.
6. Plug the spring strip onto
the pin header.
2 into the
3
1
Fig. 20Spring strip
Next stepsFinish the installation and perform the initial startup.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
29Installation
Integrator Connector
The integrator connector sends measuring signals from the
detector.
non-bipolar
4 channel
0 to 5 V
DAC 16 bit
scalable
Adjustable to offset
Power Supply
Note The nominal capacity of the connected devices must be maxi-
mum 50 % of the power supply to account for larger inrush currents when switching on the modules.
The maximum power input is 75 watts.
Prerequisites The electrical power supply at the installation site must be
connected directly to the nearest main power line.
The power must be free from ripple, residual current, voltage
peaks and electromagnetic interference.
The connectors for the mains voltage are grounded accord-
ingly.
The device receives sufficient power with reserve capacity.
The device is intended for use with AC power networks of 100 –
240 V.
Power cableUse only the enclosed power cable to connect the device to the
mains. Replace defective power cables only with accessories
from KNAUER. Only use power cables admitted for use in your
country.
In case of queries, contact the Technical Support.
CAUTION!
Electric hazard if the power adapter is turned on
while connecting or interrupting the power connection.
Switch off the power adapter beforehand.
Power plugMake sure that the power plug on the rear of the device is
always accessible, so that the device can be disconnected from
the power supply.
Next stepsFinish the installation and connect the device to the mains.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
ProcedureStainless steel fittings are tightened with 5 Nm, PEEK fittings are
Capillary layout
30Installation
Integrating the Detector into a System
To integrate the pump into a system, note the ambient conditions found in the sections Operating Environment and Technical Data as well as the ambient conditions of other devices to be
integrated into that system.
The detector is integrated into the HPLC flow system by connecting the capillary to the flow cell and the HPCL system.
tightened with 0.5 Nm.
Fig. 21Installation of the detector
Pre-Installed Capillaries
Pre-installed stainless steel and PEEK capillaries are color-coded
according to their inner diameter.
ColorMaterialInner diameter
red markerStainless steel0.1 mm
blue markerStainless steel0.25 mm
black markerStainless steel0.4 mm
red stripesPEEK0.1 mm
blue stripes PEEK0.25 mm
orange stripes PEEK0.5 mm
Note PEEK capillaries are not suitable for use with pure Acetonitrile.
Acetonitrile can cause capillaries to crack or rupture.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Operation
1
2
31Operation
In this chapter, you find information relevant for operating the
detector.
Initial Start-Up
Use this checklist to determine whether the detector is ready for
the initial start-up:
Devices is positioned in the correct location.
The power plug has been pulled.
If the device is part of an HPLC system, you should also note the
following:
The network connection to the router is established.
The chromatography software has been installed by KNAUER
or a company authorized by KNAUER.
A flow cell has been installed.
The capillaries have been connected.
Switch-On
Prerequisite The installation has been completed.
The flow cell or test cell has been inserted.
CAUTION!
Possible damage to the device caused by condensed water!
Allow device to acclimate for 3 h, before it is connected to power supply and taken into operation.
ProcedureProcessFigure
1. On the rear of the
device, plug the power
cable into the power
connector
2. Plug in the power supply.
3. On the rear of the
device, switch on the
detector using the
power switch
2.
Fig. 22Power switch with
1.
female connector at the
rear panel
ResultThe detector starts its self-validation. If the self-validation has
been successfully completed, the LED on the right and in the
center light up green.
Next stepsBring the detector into service.
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32Operation
Control
There are several options for controlling the device:
with chromatography software
with the Control Unit
with an app (Mobile Control)
NoteIt is not possible to use to control methods simultaneously. If the
device is connected to the software, it cannot be controlled via
Control Unit or Mobile Control.
Operation with Chromatography Software
To control the device with software, you have to establish a connection between the LAN port and a computer.
DAD 6.1L
Devices can be controlled with e.g. OpenLAB
version A.04.05 or higher, or with ClarityChrom version
or higher.
You find a detailed description on chromatography software in
the corresponding user manual.
®
EZChrom edition
®
5.0.3
Control with Control Unit
The Control Unit is an optional accessory. To control the DAD
6.1L using the Control Unit, connect the cable of the Control
Unit to the device. You find a detailed description on the Control Unit in its accompanying user manual.
Control with Mobile Control
The Mobile Control is an app which can be installed on your
computer or tablet computer. To control the device using the
Mobile Control, connect the computer or tablet computer to a
wireless LAN router. Data transfer between the device and the
app is actualized through wireless LAN. You find a detailed
description on the Mobile Control in its accompanying user
manual.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Legend
1234
1 Left LED
2 Center LED
3 Right LED
4 Switch/Standby but-
ton
33Operation
Meaning of the LEDs
There are three LEDs and a switch on the front of the device.
Fig. 23LEDs and switch/standby button on the front of the
device
The LEDs can have different colors depending on the operating
conditions.
StandbyTo start the standby, keep the standby button pressed for
5 seconds.
Note Malfunctioning system after repeated standby possible. After
repeatedly using standby, restart the device using the power
switch to reset the device's data storage.
ColorStatusOperation
Left LEDredError Message Check the sys-
tem
Shortly press
the switch to
deactivate the
error message.
green3D data are
acquired.
Center LEDdoes not lightThe lamp has
been switched
off.
Right LEDgreenThe device has
flashes greenThe lamp/lamps
are initializing or
the validation is
progressing.
greenThe deuterium
lamp is active.
been switched on.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Wait until the
lamp is running
or the validation is finished.
34Operation
ColorStatusOperation
flashes greenThe device not
ready for operation.
blueThe device is in
standby
Wait until the
device is ready
for operation.
Press the
standby button to end the
standby.
Re-Setting the Device
The DAD 6.1L can be reset to default settings using the Control
Unit.
ParameterSetting
NetworkLAN DHCP, port 10001
LampsD2 ON, Halogen OFF
Time constant2 s
ChannelsChannel 1: WL = 254 nm, BW = 8 nm
Reference correctionReference channel enabled for channel
1, WL = 360 nm, BW = 30 nm
Analog outOffset 1 = 0.00 mV, Scale 1 = 1 AU/V
Flow cellTest cell
Event checkAll events deactivated (o)
Date/TimeCurrent date/time
Wake-upCurrent date/time
Leak sensorON, Sensitivity = low
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
35Optimizing the Detector
Optimizing the Detector
In this chapter, you find information on how to optimize your
detector.
Location
In order to ensure thermo stability and to prevent drift effects,
note the following aspects:
Protect the detector against strong ventilation
Protect the detector against direct sunlight
Note the space requirements
Avoid vibrations
Warm-Up Time
The recommended warm-up time for the detectorsis 30 minutes. This recommendation is applicable after turning the detector on as well as and after turning the lamp/s on.
Fig. 24Cold start temperature profile
Selecting the Flow Cell
Several different flow cells are available for the detectors (see
“Flow Cells” on page 68). Note that the detector is only shipped
with a test cell. A flow cell must be ordered separately.
Signal sensitivity, peak broadening and response can all be
affected by the choice of flow cell. Important variables to take
into account are the volume and path length as well as the flow
cell material, required working pressure range, flow cell connection, and remote operation.
Flow Cell Volume
Depending on your instrument setup, column and sample(s),
one flow cell volume may be more appropriate than another. If
the flow cell is too large, two adjacent peaks may get mixed in
the cell. If the flow cell is too small, the noise may be higher and
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
36Optimizing the Detector
the signal may be too small due to less light reaching the photo
diodes.
Ideal flow cell volume therefore is a compromise between peak
broadening and sensitivity (see figure 25).
Fig. 25Volume comparison
A good rule of thumb is that flow cell volume should not be
more than 1/3 of the peak volume of your separated sample. To
determine the volume of your peaks, take the peak width as
reported in the integration results, multiply it by the flow rate
and divide it by 3.
Volumes of 2 μl, 6 μl and 10 μl are available for the detectors.
Narrow-bore columns (~ 2,1 mm ID) are suitable for flow cells
with smaller volumes. Columns with a larger inner diameter
( 3,0 mm ID) are less affected by the volume of the flow cell.
The flow rate should also be taken into consideration. A lower
flow rate increases the axial and longitudinal diffusion and adds
to a broadened flow profile which may lead to a peak broadening.
Path Length
As described by the Beer-Lambert law (see figure 26), the path
length of a flow cell affects the intensity of light that is detected.
Fig. 26Beer-Lambert law
A:Measured absorption at a wavelength
T:Transmittance, defined as the quotient of the light
intensity (I) after passing through the sample and the
initial light intensity (I
) before passing through the
0
sample.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
37Optimizing the Detector
:Molar absorptivity coefficient (wavelength- and tem-
perature-dependent)
b:Path length
c:Analyte concentration (temperature-dependent)
For the same volume of sample, the apparent concentration of
the sample will appear to be higher if the path length is longer.
Optical path lengths of 3 mm, 10 mm, and 50 mm are available
for the detectors.A longer path length increases the signal
intensity as more light is absorbed and thereby may enhance
the sensitivity of a method (see figure 27).
Fig. 27Path length comparison
Material
The flow cell materials must be chemically compatible with the
solvents and sample you are working with. Stainless steel and
metal-free flow cells are available. The metal-free variants are
bio-inert.
Pressure Stability
The different flow cells can withstand various maximum pressures. The upper pressure limits of the flow cells are 30 bar,
100 bar, or 300 bar. The flow cell should not be subjected to the
maximum pressure for a long period of time.
Connection
Undesired effects, like the loss of resolution in the chromatogram, may be prevented by ensuring a correct connection to
the flow cell and the removal of any dead volume.
Remote Operation (Fiber Optics)
The detectors can be operated with a spatially separated flow
cell which is connected with fiber optics. This provides
enhanced security for hazardous, explosive, or toxic work processes. Sensitivity is typically reduced to half that of an equivalent standard cell when working with standard length fiber optic
cables (750 mm). Overall, sensitivity is inversely proportional to
the length of the fiber optic cables.
Temperature changes around the cables may cause drift.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
38Optimizing the Detector
Selecting the Wavelength
Signal wavelengthWavelength selection can influence the sensitivity, selectivity
and linearity of a measurement. The best wavelength for a given
measurement (signal wavelength) is that which gives the maximum absorbtion above the UV cutoff of the mobile phase. In
cases where there are multiple components with different absorbance maxima, a compromise wavelength must be chosen
where all components absorb.
Reference wavelengthIn order to minimize baseline drift due to refractive index effects,
a reference wavelength can be set (see figure 28). The reference
should be set in the same spectral region as the signal wavelength (UV or VIS) but at a wavelength at which the analyte has
no absorbance.
Default reference wave-
length
By default, the reference wavelength 360 nm is activated (see
“Re-Setting the Device” on page 34). This is a suitable value for
most applications.
Fig. 28Comparison with and without reference wavelength
When selecting the signal and reference wavelengths, the
respective bandwidths must also be selected (see the following
section).
Bandwidth
The bandwidth defines the total number of wavelengths actually
registered by the photodiode when a specific wavelength is set.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
39Optimizing the Detector
1
10
------
For example, a wavelength set at 254 nm with a bandwidth of
4 nm results in an average absorbtion of 252–256 nm.
Fig. 29Bandwidth
The selection of the bandwidth is a balance between sensitivity
and selectivity. Narrow bandwidths increase selectivity, whereas
broad bandwidths increase sensitivity.
Default bandwidthBy default (see “Re-Setting the Device” on page 34), the band-
width for the signal wavelength is set to 8 nm and for the reference wavelength the bandwidth is set to 30 nm.
Spectral Range
When measuring with chromatography software, the spectral
range selected for a given measurement influences the integration time and consequently the sensitivity of the measurement,
as well as the disk space. The disk space is necessary to save generated data.
A narrow spectral range increases sensitivity and reduces disk
space. The range, however, should be wide enough to ensure
the detection of all components. Also, the spectral range always
must include the signal wavelength and the reference wavelength.
Time Constant and Data Rate
Response timeThe time constant influences the response time of the detector.
The response time determines how quickly the detector
responds to a change in signal. A good rule of thumb for selection of the detector time constant is that it should be no larger
than of the baseline peak width of the first peak of interest
(in seconds). Increasing the time constant allows more averag-
ing of the signal (also known as digital filtering) and results in
less baseline noise (see figure 30). However, increasing the time
constant too much may result in broad peaks, reduced peak
heights and asymmetric peak shapes. Therefore, a compromise
has to be found.
Time constantIn general, the best time constant is the reciprocal of the data
rate. If increased sensitivity is desired, or if the baseline noise is
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
40Optimizing the Detector
interfering with integration, the time constant should be
increased. If resolution is compromised, it should be decreased.
It is recommended to set the time constant and data rate in relation to peak width.
Peak widthTime constantData rate
[min][s][Hz]
<0.0030.01100
>0.0070.0250
>0.0170.0520
>0.0330.110
>0.0670.25
>0.1670.52
>0.33311
Data rateThe data rate (or sampling rate) is the number of data points per
second (Hz) at which the detector transmits data to the computer.
Default data rateThe default data rate setting for the detectors is 1 Hz (see “Re-
Setting the Device” on page 34). The maximum data rate (digital signal) is 100 Hz. Slower data rates store average data points.
A 50 Hz data rate averages 2 points. A 10 Hz data rate averages
10 points. The analog data rate is fixed at 12.5 Hz.
Optimizing the
data rate
The optimal data rate depends on your application. Too few
points across a peak (short data rate) decrease detail and compromise reproducibility. Too many points (high data rate) introduce noise into the system and the resulting files can become
very large. Some general considerations are listed below:
Each peak should be defined by 20–30 data points. For chro-
matograms with co-eluting peaks or low signal-to-noise
ratios, 40–50 data points per peak are recommended.
If all peaks are relatively wide, select a slower data rate.
If any peaks of interest are less than a few seconds, select a
faster data rate.
If the data rate is too slow, the start and end points of the peaks
are not accurately determined. If the data rate is too fast, data
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
41Optimizing the Detector
files may occupy excessive disk space and post-run analyses may
require more processing time.
Fig. 30Noise with different data rates
Integration time
Signal levelThe integration time influences the intensity of the signal and
therefore the sensitivity of the measurement. The larger the integration time, the higher the intensity of the signal until the maximum sensor counts are reached. The integration time is
automatically calculated by the software before the start of a
measurement. The calculations are in relation to the spectral
range (see section “Spectral Range” on page 39).
When a narrower spectral range is selected, the signal intensity
will be increased. This increase is limited however by the data
rate.
Subtraction of the Baseline Chromatogram
The baseline subtraction can eliminate the effects of drift that
result from solvent, gradient, or flow programming. The baseline profile is subtracted from the measured chromatogram. This
results in a mathematically reprocessed chromatogram with an
ideally flat baseline.
General
The performance of the detector is largely dependent on the
performance of the HPLC system.
Noise can be related to pump stability, the detector cell
cleanliness, lamp quality, mobile phase composition and
other factors.
Drift is usually related to long-term changes in the environ-
ment, such as detector warm-up or fluctuations in temperature and mobile phase composition.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
42Functionality Tests
Functionality Tests
Installation Qualification (IQ)
Installation reportCertification on the functionality of the device. During installa-
tion of the device, an installation report (IQ document) is created upon request in coordination with the Technical Support of
KNAUER.
This installation report needs to be completed in full and signed
by both parties. It serves as proof of the properly executed
installation and functionality of the device.
Operation Qualification (OQ)
Extensive
functionality test
Test IntervalsRun the extensive functionality test at the following time inter-
ExecutionThe test can carried out either by KNAUER's Technical Support
Extensive test of the device’s functionality. A successfully executed OQ ensures that the device functions properly.
vals:
Average useDevice test
1 to 5 days/week:Every 6
months
More than 5 days/week or 24 hours/day:Every 3
months
Operation with buffer solutions or other salt
solutions:
or by a technical service authorized by KNAUER.
The OQ documentation required for executing the OQ is with
costs (once) and can be ordered separately from KNAUER.
Every 3
months
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
43Troubleshooting
Troubleshooting
First Measures1. Check all cabling.
2. Check all screw fittings.
3. Check whether air has gotten into the supply lines.
4. Check device for leaks.
5. Pay attention to system messages.
LAN
In case no LAN connection between the computer and the
devices can be established, go through the following steps.
Check after each step if the problem is solved. If the problem
cannot be located, call the Technical Support.
1. Check the status of the LAN connection in the Windows task bar:
Connected
Connection not established
If no connection was established, test the following:
Is the router switched on?
Is the patch cable connected correctly to the router
and the computer?
2. Check the router settings:
Is the router set to DCHP server?
Is the IP address range sufficient for all the connected
devices?
3. Check all connections:
Are the patch cables connected to the LAN ports and
not the WAN port?
Are all cable connections between devices and router
correct?
Are the cables plugged in tightly?
4. If the router is integrated into a company network,
pull out the patch cable from the WAN port.
Can the devices communicate with the computer,
even though the router is disconnected from the company network?
5. In case you own a Control Unit, check the settings in
the menu Setup > Network.
Is LAN-DHCP set for controlling?
Did the device receive an IP address?
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
44Troubleshooting
6. Turn off all devices, router, and computer. Firstly, turn
on the router and secondly turn on the devices and
the computer.
Has this been successful?
7. Replace the patch cable to the device that no connection could be established with.
Has this been successful?
8. Ensure that the IP port of the device corresponds to
the IP port set in the chromatography software.
Possible Problems and Solutions
ProblemSolution
Baseline driftMaintain constant temperature condi-
tions during the measurement.
Device cannot be
switched on
Device cannot be
calibrated
Baseline noise1. Inspect the flow-cell assembly.
The relationship of
the signal to the
light path reference
is very low
Further Measures Install the maintenance software (service tool).
Save device information and send to KNAUER.
Inform the KNAUER Technical Support.
Inspect the power cable to ensure that
it is plugged into the power supply.
1. Insert the test cell.
2. Inspect the calibration with a weak
absorbing solvent.
2. Exchange the defective flow cell.
3. Inspect the service life of the lamp
on the display.
4. Reduce the air in the flow cell by
using a degasser.
1. Flush the flow cell.
2. Replace the lamps
System Messages
If other system messages are displayed besides those listed
below, please turn the device off and then on. Inform the Technical Support of the manufacturer in case the system message
repeats itself.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
45Troubleshooting
The system messages are in alphabetical order:
System messageError List and Solutions
A“Ambient tempera-
ture sensor failed”
C“Cannot initialize
LAN”
“Instrument not validated”
“Cannot proceed:
D2 lamp heating”
“Cannot proceed:
D2 lamp off”
“Cannot proceed:
Low light”
Main board temperature sensor not
detected or does not respond.
Check cables and connections in local
area network.
Data aquisition or single scan cannot
be executed when the instrument is
not validated.
Manual validation cannot be executed
during lamp ignition. Wait for the
deuterium lamp to finish the initialization process, then continue.
Manual validation can not be executed while deuterium lamp is off.
Switch on the lamp. If the error occurs
again, restart the device.
If the error message re-appears,
replace the lamp.
Validation failed due to integration
time exeeding limits. If the recommended operating time of the lamp
has been exceeded. Replace the deuterium lamp.
If the flow cell is dirty. Clean the flow
cell.
Finally, if the optical system is faulty.
Inform the KNAUER Technical Support.
D“D2 lamp failed”Manual validation cannot be executed
when no deuterium lamp is present.
“Communication
RS-232 communication disrupted.
buffer overflow”
“Communication
timeout”
Timeout: RS-232 (5 s), Leak sensor
(0.5 s) or hardware component (lamp
power supply, EEPROM, I2C with GUI)
communication failure
“CRC failure”Correct command, disruption during
communication (not currently active).
”D2 lamp operation
failed”
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Restart the device. If the error occurs
again, replace the lamp.
46Troubleshooting
System messageError List and Solutions
”D2 lamp does not
start”
Switch off lamp on touchscreen and
restart. Inform the KNAUER Technical
Support in case the system message
repeats itself. Replace the lamp unit.
Upon device start up or manual validation attempt, lamp does not ignite.
”Data acquisition
active”
No entries are possible. First stop
acquiring measurement data, afterwards you can make a new entry.
E"Error input acti-
vated"
Error outside of the device. Check the
external devices and cable connections.
Check the system to localize and
resolve the source of the external
error.
H”HAL lamp does not
start” (DAD 6.1L
only)
Switch on the lamp. If the error occurs
again, restart the device.
If the lamp is off after restarting,
replace the lamp.
I“Instrument busy”Attempt to scan while 3D data acqui-
sition is active, leak sensor or shutter is
busy processing previous command.
Wait until the device has complete
processing.
“Instrument in
standby mode”
“I2C lamp(s) operation failed”
“I2C operation
failed”
Command not allowed for standby
mode.
Lamp power supply I2C communication failure.
Leak sensor or lamp power supply
firmware upload or EEPROM I2C communication failure.
“Instrument in
standalone mode”
”Instrument remote
controlled”
“Insufficient access
for operation”
Command cannot be executed in
local modus (only in remote modus)
This entry is not executable. Quit software.
Lamp power supply data cannot be
edited (service mode should be activated)
”Invalid command”Check the cable connections. Change
the entry.
Wrong command sent to device.
Check the validity of the command.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
47Troubleshooting
System messageError List and Solutions
“Invalid parameter(s)”
Correct command, incorrect parameter. Check the validity of the parameters
L“Lamp cover open”Protective lamp cover not correctly
attached or micro switch malfunction.
Attach the lamp cover.
“Lamp not installed”GLP data and lamp operation cannot
be performed as this lamp (D2 or halogen) is not installed.
“Lamps off”Command cannot be carried out
while the lamps are turned off.
“Lamp supply is not
available”
“Lamp supply temperature limit
Lamp supply not installed or does not
respond.
Upper temperature limit for lamp
power supply has been reached.
exceeded”
“Lamp unit temperature limit
Upper temperature limit for lamp unit
has been reached.
exceeded”
“Lamp unit temperature limit
exceeded”
“Leak sensor failed”Leak sensor not detected or does not
"Leak was detected"Switch off the device. Remove the leak
N“No D2 lamp
detected”
“Not available for
this communication
mode”
R“Recommended D2
lamp life exceeded”
“Recommended
HAL lamp life
exceeded”
Main board temperature sensor not
detected or does not respond.
respond. Restart the device.
If the leak sensor is still not present,
contact the Technical Support of the
manufacturer.
and restart the device.
Make sure that the deuterium lamp
has been installed correctly.
3D data aquisition or single scan is not
available over RS-232.
The recommended 2000 hours for the
deuterium lamp has been exceeded.
Replace the lamp.
The recommended 1000 hours for the
halogen lamp has been exceeded.
Replace the lamp.
S“Shutter position
Shutter filter motor malfunction.
failed”
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
48Troubleshooting
System messageError List and Solutions
“Spectrum buffer
overflow”
“Spectrum output
busy or not ready”
T“Temperature con-
trol failed”
”Time already
exists”
W“Wake up time
already passed”
“WL/BW out of
spectral range”
”WL validation
failed”
Internal 3D data buffer is exhausted
due to LAN connection failure.
3D data acquisition cannot be started.
Wait until the data transfer is complete before continuing.
Maximum temperature on lamp unit
exceeded.
Correct the time entry.
The wake up entry is set in the past.
Enter a new time.
Selected wavelength and bandwidth
exceed the spectral range. Extend the
selected spectral (scan) range or
change the selected wavelength and /
or bandwidth.
The wavelength precision validation
has failed. Restart the validation. Holmium oxide and/or H
, H lines out of
specification.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
49Maintenance and Care
Maintenance and Care
In this chapter, you find all steps necessary for maintenance,
care, and storage. Additionally, you find instructions for maintenance tasks that you may perform yourself. If maintenance tasks
are necessary that are not described here, contact your dealer or
the Technical Support.
Organic solvents are toxic above a certain concentration. Ensure
that work areas are always well-ventilated! When performing
maintenance tasks on the device, always wear safety glasses with
side protection, protective gloves, and a lab coat.
All wetter components of a device, e.g. flow cells of detectors or
pump heads and pressure sensors for pumps, have to be flushed
with isopropanol first and water afterwards before being maintained, disassembled or disposed.
Opening the deviceThe device may only be opened by the KNAUER Technical Sup-
port or any company authorized by KNAUER.
WARNING!
Irritation of retina through UV light! Concentrated UV light can leak out from the flow cell or
the fiber optic connectors.
Switch off the device or the lamps.
CAUTION!
Performing maintenance tasks on a switched on
device can cause damage to the device.
Switch off the device and pull the power plug.
DANGER!
Danger of electric shock! Voltage-carrying parts
inside the device.
Before opening the housing, be sure to switch off
the device and pull the power plug.
Users may perform the following maintenance tasks themselves:
Regularly checking the operating hours of the lamp.
Checking the flow cell installation.
Replacing the flow cell
Replacing the lamp
Proper maintenance of your HPLC device will ensure successful
analyses and reproducible results.
Find the order numbers of the required spare parts in the chapter Accessories and Spare Parts.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
50Maintenance and Care
Contact the Technical Support
If you have any technical questions regarding KNAUER hardware
or software, please use one of the contact options below:
Technical Support hotline:
European hotlineLanguages: Available by telephone
in German or English:8 am to 5 pm (CET)
Phone:+49 30 809727-111
Telefax:+49 30 8015010
E-mail contact:support@knauer.net
Maintenance Contract
The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is
covered by a separate maintenance contract:
• Opening the device or removing housing parts.
Maintenance Intervals
Operating hoursThe Control Unit, the Mobile Control and the different software
®
products (e.g. ClarityChrom
play or read out the operating time of the detector. You find a
detailed description on how to display or read out GLP data in
the respective user manuals.
Operating hoursMeasures
1000 Replace halogen lamp (DAD 6.1L
2000 Replace deuterium lamp
or OpenLAB®) enable you to dis-
only)
Cleaning and Caring for the Device
CAUTION!
Intruding liquids can cause damage to the device!
Place solvent bottles next to the device or in a
solvent tray.
Moisten the cleaning cloth only slightly.
All smooth surfaces of the device can be cleaned with a mild,
commercially available cleaning solution, or with isopropanol.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
51Maintenance and Care
Checking the Fittings
Check if all fittings are tight. If fittings are not tight, re-tighten
fittings.
Capillary fittingsTorque
Stainless steel fittings5 Nm
PEEK fittings0.5 Nm
Putting the Device out of Operation
The detector is designed for use with different solvents. If the
detector is out of operation for several weeks, residual solvent
can cause damage. Therefore, it is recommended to:
flush the flow cell, the capillary, and the detector
remove the used solvent completely
fill the flow cell, the capillary, and the detector with isopropa-
nol
Seal the open connectors of the flow cell with cap fittings.
PrerequisiteThe detector head has been purged.
ToolsCap fittings and/or screw-type cap fittings
ProcedureProcess
1. Remove the solvent tubing and seal all open connectors
with cap fittings.
2. Disconnect the detector from the HPLC flow system and
seal the open connectors of the flow cell with cap fittings.
Next stepsChoose a storage location according to the ambient conditions
listed in the respective chapter of this user manual.
Storage
Note that prior to storing all tubes and capillary should be emptied or filled with an appropriate flushing solution (e.g. isopropanol). To prevent algae formation, do not use pure water. Seal
all inlets and outlets with cap fittings.
Note the ambient conditions for storage (See “Ambient conditions” on page 58, 59, 60).
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
52Maintenance and Care
Disconnect from Power Supply
PrerequisiteThe device has been switched off.
Procedure
Next stepsDisconnect all power connectors. Remove the flow cell and pack
Process
1. Pull the power plug from the socket and afterwards
remove from the device.
2. Pack the power cable with the device.
the device for transport or storage.
Cleaning the Flow Cell
Increased noise of the baseline and reduced sensitivity can be a
result of a dirty flow cell. Often it is sufficient to rinse the flow
cell to restore optimal sensitivity.
Rinsing the Flow Cell
CAUTION!
Contamination of the flow cell caused by oil
drops! Do not use compressed air for drying.
The following solvents are recommended for rinsing:
2. Inject it into the inlet of the flow cell and allow it to act for
5 minutes.
3. Fill the syringe with water and flush again.
4. Remove the flow cell from the detector and use a nitrogen
stream to dry it.
Next stepsCheck if the baseline noise has disappeared.
Replacing the Flow Cell
UV light will cause the flow cells to become blind with time
(solarization), making them no longer suitable for use. The manufacturer recommends replacing the flow cell after about
6000 operating hours.
Prerequisite The capillaries are disconnected.
The device is switched off.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
53Maintenance and Care
1
4
3
1
2
WARNING!
Irritation of retina through UV light! Concentrated UV light can leak out from the flow cell or
the fiber optic connectors.
Switch off the device or the lamps.
ProcedureStepsFigure
1. Press the release
1 down.
lever
2. Remove the flow
cell.
3. Insert the new flow
cell until it locks into
place.
Next stepsConnect the capillaries.
Fig. 31Releasing the flow cell
DAD 6.1L, DAD 2.1L,
MWD 2.1L
DAD 6.1L The recommended operating time of the lamp is 1000 hours.
Legend
1 Deuterium lamp con-
nector
2 Halogen lamp connec-
tor (DAD 6.1L only)
3 Halogen lamp (DAD
6.1L only)
4 Deuterium lamp
Replacing the Lamp
Replace the lamp if it malfunctions or its intensity is low.
The recommended operating time of the lamp is 2000 hours.
Fig. 32Overview deuterium and halogen lamp
Removing the Deuterium or Halogen Lamp
Prerequisites The device is switched off.
The lamp has cooled down.
ToolScrewdriver Allen wrench, 2.5 mm
NoteIf the device is still in operation, the integrated security switch
turns off the lamp automatically on opening the lamp cover. An
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
54Maintenance and Care
1
2
3
4
5
6
error message is displayed. In addition, the red LED lights up
and the center LED does not light.
DANGER! Risk of death due to high voltages.
Do not change lamps if the lamp area is not voltage-free.
Check the status of the lamps in the software and
on the LEDs.
WARNING! Burn hazard from hot lamp!
Switch off the lamp meanwhile the device
remains switched on for further 15 minutes, otherwise the lamp can not cool down.
Afterwards, switch off the device and pull the
power plug.
ProcedureProcessFigure
Deuterium lamp
1. Loosen the screws
2 of the lamp
and
cover.
2. Remove the lamp
cover.
3. Loosen the lock ring
3 or 4 of the lamp
plug and pull out
the plug.
4. Using the screwdriver, loosen the
two screws
5 or 6.
1
Fig. 33Lamp cover
Fig. 34Lock ring of the lamp plug
5. Remove the lamp.
Fig. 35Screws on the lamp socket
Next stepsInsert a new lamp.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
55Maintenance and Care
1
2
3
7
6
4
5
Installing the Deuterium or Halogen Lamp
Removing the lamp cover switches off the device.
Prerequisites The device is switched off.
The lamp cover has been removed.
The old lamp has been removed.
ToolScrewdriver Allen wrench, 2.5 mm
CAUTION!
Damage to the lamp and inaccurate measuring
results due to residue possible!
Do not touch the glass body with your hands!
Wear gloves.
Use a clean, soft cloth.
ProcedureProcessFigure
1. Hold the lamp at the
lamp socket and
guide the glass body
into the lamp pod
1.
2. The bolt
deuterium lamp sits
in a notch in the
lamp socket.
Note
Insert the halogen lamp
2 of the
3 into the lamp pod at
a slight angle.
3. Using the screwdriver, tighten the
two screws
on the lamp socket.
4. Connect the plug
or 6 and tighten
the lock ring.
4 or 7
5
Fig. 36Lamp pod of the D2 lamp
Fig. 37Lamp pod of the halogen
lamp
Fig. 38Installing the deuterium
lamp
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
56Maintenance and Care
8
9
ProcedureProcessFigure
5. Attach the lamp
cover and fix with
the screws
8 and
9.
Fig. 39Lamp cover
Next stepsBring the device into operation.
Removing a Leak
PrerequisiteIf the leak tray is filled with liquid, the leak has to be removed.
Auxiliary materialCloth for drying the leak sensor
ProcedureProcess
1. Remove the leak
2. Dry the leak tray.
3. Acknowledge the error message via Mobile Control, Control Unit, the software of the standby switch (without software control).
Next stepsBring the device into operation.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Technical parametersGLP functionDetailed report including lamp recog-
nition, operating hours, lamp operating hours, number of lamp ignitions.
DisplayMobile Control
Ambient conditions
Temperature range4–40 °C, 39.2–104 °F
Humiditybelow 90 %, non-condensing
GeneralPower supplyInput 100–240 V
Output 50–60 Hz
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
61Technical Data
Maximum power
consumption
Dimensions361 mm x 158 mm x 523 mm
Weight13.8 kg
Leak sensorYes
Degree of protectionIP 20
Height above sea
level
75 Watt
(Width × Height × Depth)
maximum 2000 meters
Conditions for Specifications
The technical data has been determined in accordance with the
ASTM standard E1657-94.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Disposal
62Disposal
Hand in old devices or disassembled old components at a certified waste facility, where they will be disposed of properly.
AVV marking in Ger-
many
WEEE registrationKNAUER as a company is registered by the WEEE number DE
Solvents and other oper-
ating materials
According to the German "Abfallverzeichnisverordnung" (AVV)
(January, 2001), old devices manufactured by KNAUER are
marked as waste electrical and electronic equipment: 160214
34642789 in the German "Elektroaltgeräteregister" (EAR). The
number belongs to category 8.
All distributors and importers are responsible for the disposal of
old devices, as defined by the WEEE directive . End-users can
send their old devices manufactured by KNAUER back to the distributor, the importer, or the company free of charge, but
would be charged for the disposal.
All solvents and other operating materials must be collected separately and disposed of properly.
All wetted components of a device, e. g. flow cells of detectors
or pump heads and pressure sensors for pumps, have to be
flushed with isopropanol first and water afterwards before being
maintained, disassembled or disposed.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
63Legal Information
Legal Information
Warranty Conditions
The factory warranty for the device is valid for 12 months after
the date of dispatch. All warranty claims shall expire in the event
that any unauthorized changes are made to the device.
During the warranty period, any components with material or
design-related defects will be replaced or repaired by the manufacturer free of charge.
This warranty excludes the following:
Accidental or willful damage
Damage or errors caused by third parties that are not con-
tractually related to the manufacturer at the time the damage
occurs
Damage caused by negligence or improper operation of the
device and damage caused by clogged capillary
Packaging and transport damage
In the event of device malfunctions, directly contact the manufacturer.
Wissenschaftliche Gerätebau
Dr. Ing. Herbert Knauer GmbH
Hegauer Weg 38
14163 Berlin, Germany
Phone:+49 30 809727-111
Telefax:+49 30 8015010
e-mail:info@knauer.net
Internet:www.knauer.net
Transport Damage
The packaging of our devices provides the best possible protection against transport damage. Check the devices for signs of
transport damage. In case you notice any damage, contact the
Technical Support and the forwarder company within three
workdays.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
64HPLC Glossary
HPLC Glossary
In the following chapter you find abbreviations and terminology
that is used in HPLC.
TermDefinition
AbsorptionA substance retaining light of certain wave-
lengths when being shone at.
AdsorptionThe stationary phase attracting the molecules
of separated substances.
AnalyticalQualitative analysis of samples in HPLC (see:
preparative)
Backflushing
CalibrationProcess for correcting measuring values by the
CapillaryThin metal or PEEK pipe that connects the
Carrier
material
Chromatogram
Control
Unit
ColumnThe pipe with two end fittings which is perme-
Correction
factor
Flushing of columns and pre-columns by
reversing the flow direction. This enables
strongly retarding substances to be separated.
value that a measuring device deviates from
the standard.
components and devices inside of an HPLC system.
Solid particles which carry the stationary phase
(packing material).
The record of a detector signal, depending on
the flow volume of the mobile phase or time.
Hand-held device with a touchscreen to drive
single AZURA devices locally.
able for solvent. The pipe is filled with packing
material.
Factor that arithmetically corrects devicerelated deviations from measuring values.
Dead volume
DegasserDegasser module for fluids
DetectorDevice measuring the composition or the
Flow cellMeasuring cell
GLPQuality assurance system for laboratories
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Volume of capillary and system components
between mixer, injector and column as well as
between column and detector.
quantity of a substance
(Good Laboratory Practice)
65HPLC Glossary
TermDefinition
GradientTime-dependent composition of solvent
GroundProtective measure for electro-conductive
HPLCHigh Pressure Liquid Chromatography (HPLC)
isocraticMode of sample separation where the compo-
(mobile phase) on low-pressure or high-pressure side of system
housing parts
sition of a solvent remains constant
KNAUER
LightGuide
KNAUER LightGuide flow cell cartridges combine a maximum light transmission (using total
reflection) with a minimum cell volume to offer
an ideal signal-to-noise ratio.
Luer-Lock
cannula
Mobile
Control
Mobile
phase
A standardized connector between syringes
and cannulas
Mobile application to control and program the
whole HPLC system via wireless LAN.
Flowing agent that transports the substances
to be separated or isolated through the column
(solvent).
Packing
material
Solid particles which carry the stationary phase
(carrier material).
PeakThe deflection of a detector for an analyte in a
chromatogram.
pHMeasure for the acidic or alkaline character of
an aqueous solution.
pH elec-
pH sensor
trode
PreparativeIsolating the maximum amount of a substance
in a short amount of time in a required purity.
Response
time
The time required, after a stepwise change in
the composition in the detector cell, for the
output signal to reach a predetermined percentage (67%) of the new equilibration value.
For example, if the time constant is 1 s, it will
take 1 s to attain 67% if the value of the new
signal.
Retention
time
The time required from injection of a substance
until the maximum concentration of a substance becomes visible.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
66HPLC Glossary
TermDefinition
SampleA mixture of different components which are
to be separated via chromatography. The components are moved by the mobile phase and
dissolved from the column.
Sample
loop
A loop which is separate from a chromatographic system and which the sample is
injected to. After a switch is actuated, the solvent flow passes the loop and the sample is
flushed onto the column.
SolventFlowing agent that transports the substances
to be separated or isolated through the column
(mobile phase)
Stationary
phase
The stationary substance in a chromatographic
system on which the substance to be separated
settles. It is the liquid or the liquid film on the
surface of the packing material.
Temperature compensation
The settings under Temperature compensation
equalize the changing measuring values with
the ambient temperature.
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
67Repeat Orders
Repeat Orders
Note For repeat orders of spare parts use the enclosed packing list.
Contact the Technical Support in case there are any questions
on spare parts or accessories.
Further InformationFind further information regarding spare parts and accessories at
www.knauer.net.
Modules
NameOrder number
DAD 6.1LDiode Array Detector DAD 6.1L without
flow cell
Test cellAMLX8
DAD 2.1LDiode Array Detector DAD 2.1L without
flow cell
Test cellAMLX8
MWD 2.1LDiode Array Detector MWD 2.1L without
flow cell
Test cellAMLX8
ADC11
ADC01
ADB01
Accessories and Spare Parts
NameOrder number
User ManualUser Manual EN/DEV6700
Operation
Qualification
DAD 6.1L
DAD 2.1L
MWD 2.1L
VOQDAD61L
VOQDAD21L
VOQMWD21L
Installation
Qualification
LampsHigh-intensity deuterium lampAZL01
Drainage system Corrugated hose 16 cm, PE gray A9846-1
Capillary guide Capillary guide top P6424
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
DAD 6.1L
DAD 2.1L
MWD 2.1L
Halogen lampAZL02
Funnel P6431
Exhaust P6432
Capillary guide side P6425
VIQDAD61L
VIQDAD21L
VIQMWD21L
68Repeat Orders
NameOrder number
Control Unit Control Unit CU 2.1, incl. user manual (for
Accessories kit AZURA accessories kit FZA02
ToolsTool Kit AZURAA1033
KNAUER LightGuide
Flow Cell Cartridge
KNAUER LightGuide
High Sensitivity
Flow Cell Cartridge
AZD00
DAD 6.1L only)
DAD 6.1L, DAD 2.1L, MWD 2.1L accessories kit
FDC
Flow Cells
Technical DataOrder number
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
10 mm
1/16“
2 μl (0.8 μl dispersion vol.)
PEEK, quartz, teflon
5 ml/min
100 bar
50 mm
1/16“
6 μl (2 μl dispersion vol.)
PEEK, quartz, teflon
5 ml/min
100 bar
AMC19
AMD59
Flow Cells with Fiber Optics Connectors
Technical DataOrder number
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
10 mm
1/16“
10 μl
stainless steel, quartz,
PEEK
20 ml/min
300 bar
3 mm
1/16“
2 μl
stainless steel, quartz,
PEEK
50 ml/min
300 bar
A4130
A4131
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
69Repeat Orders
Technical DataOrder number
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
3 mm
1/16“
2 μl
PEEK, quartz, PTFE
50 ml/min
30 bar
0.5 /1.25 /2 mm
1/8“
1.7/4.3/6.8 μl
stainless steel, quartz,
PTFE
1000 ml/min
200 bar
0.5 /1.25 /2 mm
1/8“
1.7/4.3/6.8 μl
stainless steel, quartz,
PTFE
1000 ml/min
100 bar
0.5 /1.25 /2 mm
1/4“
1.7/4.3/6 μl
stainless steel, quartz,
PTFE
10000 ml/min
200 bar
A4132
A4133
A4134
A4135
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
Path length
Connection
Volume
Material
Max. flow rate
Max. pressure
Fiber Optics AdapterNecessary for connecting the fiber opticsAMKX8
0.5 mm
1/16“
1.7 μl
stainless steel, quartz,
PEEK
250 ml/min
200 bar
0.5 mm
1/16“
1.7 μl
PEEK, quartz, PTFE
250 ml/min
100 bar
A4136
A4137
Fiber Optics
NameOrder number
2 x fiber optic cables 750 mmA0740
2 x fiber optic cables, customizedA0743
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
flow cells 68
Re-Setting 34
Response time 65
Retention time 65
Router (LAN) 24
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
73Index
S
Safety 12
Safety equipment 12
Salts 9
Sample 66
Sample loop 66
Selectivity 39
Self-calibration 31
Sensitivity 37, 39
Service 50
Setup 17
Side View 7
Signal sensitivity 35
Signal wavelength 38, 39
Software 50
Solvent 66
flammability 13
line 13
self-ignition point 13
toxicity 13
tray 13
Solvents 9
suitable 9
Space Requirements 17
Space requirements 16
Spare parts 11, 67
Standby 33
Standby button 33
Start IN, see pin header 27
Stationary phase 66
Storage 49, 51
Subtraction of the baseline 41
Switch-On 31
Symbols and Signs 15
System integration 30
System Messages 44
T
Technical Data 57
DAD 2.1L 58
DAD 6.1L 57
MWD 2.1L 59
Technical data
detector
Technical Support 50
Temperature compensation 66
Tests
Intervals
Time constant 39
Transport damage 63
Troubleshooting 43
LAN 43
system messages 44
57
42
U
Unpacking 17
USB port 7
W
Warm-Up Time 35
Warranty 63
Wavelength
cut-off
select 38
38
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Declaration of Conformity
Manufacturer Wissenschaftliche Gerätebau
Dr. Ing. Herbert Knauer GmbH
Hegauer Weg 38
14163 Berlin, Germany
Product: Detector DAD 6.1L, DAD 2.1L,
MWD 2.1L
Order numberEDC11EDC01EDB01
Designation 1
Designation 2
The product complies with the following requirements and
product specifications:
Europe
DAD 6.1LDAD 2.1LMWD 2.1L
Diode array detector DAD 6.1LDiode array detector DAD 2.1LVariable multi wavelength
DIRECTIVE 2006/42/EC OF THE EUROPEAN PARLIAMENT
AND OF THE COUNCIL of 17 May 2006 on machinery, and
amending Directive 95/16/EC (recast)
for electrical equipment for measurement, control and
laboratory use - Part 2-081: Particular requirements for
automatic and semi-automatic laboratory equipment for
analysis and other purposes
UV/VIS detector MWD 2.1L
RoHS directives 2002/95/EC (2003) and 2011/65/EU on
the restriction of the use of certain hazardous substances
in electrical and electronic equipment
WEEE directive 2002/96/EC (2003) and 2012/19/EU on
waste electrical and electronic equipment
Canada
UL 61010 - 1: 2012 (2012-05-11) Safety requirements for
electrical equipment for measurement, control, and laboratory use - Part 1: General requirements
Auf der Vorderseite des Geräts befindet sich die Durchflusszelle
und die Lampenklappe zum Austausch der Lampen.
Abb. 1Vorderansicht (Beispiel DAD 6.1L)
Rückansicht
RS-232, Service-Schnitt-
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
stelle
Auf der Rückseite des Detektors finden Sie neben den Anschlüssen für die LAN-Verbindung und den Netzstecker auch die Seriennummer sowie den Netzschalter zum Ein- und Ausschalten
des Gerätes.
Die Service-Schnittstelle ist ausschließlich für Wartungs- und
Reparaturarbeiten durch einen Service-Techniker zu verwenden.
Legende
2345
6
71
1
1 Geräteinformatio-
nen
2 Integratorausgänge
3 Anschluss LAN
4 Schnittstelle für den
Service
5 Stiftleiste
6 Lüfter
7 Netzanschluss mit
Netzschalter
7Bestimmungsgemäße Verwendung
Abb. 2Rückansicht (Beispiel DAD 6.1L)
Seitenansicht
DAD 6.1LAn der Seite des Detektors befindet sich der Anschluss, mit dem
Hinweis Der USB-Anschluss für den DAD 2.1L und den MWD 2.1L ist
Legende
1 USB-Anschluss für
die Control Unit
die Bedieneinheit Control Unit (siehe ’Bedienung mit Mobile
Control’ auf Seite 33.) verbunden wird.
nicht aktiv. Die Detektoren können nicht mit der Control Unit
gesteuert werden.
Abb. 3USB-Anschluss für die Control Unit
Leistungsübersicht
Der Detektor dient in einem HPLC-System dazu, Stoffe in Flüssigkeiten nachzuweisen und ihre Konzentration anzugeben. Die
Empfindlichkeit des Detektors hängt von der eingebauten
Durchflusszelle ab.
Optisches SystemDie Detektoren messen eine Lichtabsorption durch die Probe im
ultravioletten und visuellen Bereich. Das gesamte Spektrum wird
durch die Probe gesendet und danach durch ein Gitter aufgespalten. Das aufgespaltene Licht fällt auf eine geometrische
Anordnung von 1024 (DAD 6.1L) oder 256 (DAD 2.1L, MWD
2.1L) einzelnen Photodioden, dem Photodiodenarray.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
Legende
3
5
1
2
4
6
1 Deuteriumlampe
2 Halogenlampe
3 Durchflusszelle
4 Diodenarray-Sensor
5 Diffraktionsgitter
6 Spiegel
8Bestimmungsgemäße Verwendung
Das optisches System des AZURA DAD 6.1L Detektors ist in
Abbildung 4 dargestellt. Eine aktive Temperaturkontrolle mit
0,5°C Empfindlichkeit sichert gute Basislinienstabilität für eine
minimale Basisliniendrift.
Abb. 4Optisches System DAD 6.1L
LampenBeide Lampen können ohne Service Techniker schnell und ein-
fach von vorne gewechselt werden.
DAD 6.1LZwei Lichtquellen, eine Deuterium- und eine Halogenlampe
decken den gesamten UV-Vis Wellenlängenbereich von
190–1020 nm ab.
DAD 2.1L, MWD 2.1LEine Deuteriumlampe deckt den UV Wellenlängenbereich von
190–500 nm ab.
Lampenspiegel
DAD 6.1L
DurchflusszelleEine Vielzahl unterschiedlicher Durchflusszellen für verschiedene
KNAUER LightGuide
Messzellkartuschen
Eine effektive Lichtmischung der Deuterium- und Halogenlampen durch einen Spiegel mit Polka Dot Technologie ermöglicht
eine maximale Lichtintensität über den gesamten Wellenlängenbereich.
Anwendungen stehen zur Verfügung. Messzellkartuschen mit
Standard und KNAUER LightGuide Technologie sind erhältlich.
KNAUER LightGuide Messzellkartuschen kombinieren eine maximierte Lichtausbeutel (durch Totalreflexion) mit einem minimalen Zellvolumina um ein optimales S/N-Verhältnis zu
gewährleisten.
Adapter für Lichtwellenleiter sind zusätzlich erhältlich. Lichtwellenleiter erlaubten die räumliche Trennung von Messzelle und
Detektor. Dies erhöht die Sicherheit bei Arbeitsprozessen mit
gefährlichen, explosiven oder toxischen Materialien.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
9Bestimmungsgemäße Verwendung
DatenrateDer Detektor misst mit einer maximalen Datenrate von 100 Hz.
Dabei werden pro Sekunde ca.100 Spektren aufgenommen.
LeckmanagementEingebautes Leckmanagement zum Ableiten von austretenden
Flüssigkeiten.
GLP-DatenMit Hilfe der Mobile Control sowie der verschiedenen Software-
pakete (ClarityChrom
®
oder OpenLAB
®
) ist es möglich, die GLPDaten wie Betriebsstunden, Lampen-Betriebsstunden, Anzahl
der Lampenzündungen, etc. aus dem Detektor anzuzeigen oder
auszulesen. Dem DAD 6.1L steht zusätzlich die Control Unit zum
Auslesen der GLP-Daten zur Verfügung. Eine detaillierte
Beschreibung zum Anzeigen oder Auslesen der GLP-Daten ist im
jeweiligen Benutzerhandbuch zu finden.
Weiteres ZubehörAls weiteres Zubehör sind folgende Artikel erhältlich:
Die Mobile Control App, für Android und Windows 8, zur
Bedienung
Die Control Unit zur Bedienung des DAD 6.1L
Eluenten
Schon die Zugabe kleiner Mengen anderer Substanzen wie
Additive, Modifier oder Salze können die Beständigkeit der
Materialien beeinflussen.
In Zweifelsfällen kontaktieren Sie die technische Kundenbetreuung.
Methanol
Phosphatpufferlösungen (0,5 M)
Toluol
verdünnte ammoniakalische Lösung
verdünnte Essigsäure (z. B. 0,1–1 %) bei 25 °C
verdünnte Natronlauge (1 M)
Wasser
1
)
2
1) gültig im angegebenen Temperaturbereich
2) nicht in Kombination mit PEEK-Kleinteilen oder PEEK-Kapillaren zu empfehlen
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
Perfluorierte Eluenten, z. B. Fluorinert
Perfluorierte Polyether, z. B. Fomblin
FC-75, FC-40
®
Salzsäure
verdünnte Natronlauge (1 M)
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
11Lieferumfang
Lieferumfang
Hinweis Ausschließlich Ersatzteile und Zubehör vom Hersteller oder einer
DAD 6.1LDetektor
BeipackDAD 6.1L
DAD 2.1LDetektor
vom Hersteller autorisierten Firma verwenden.
Netzkabel
Benutzerhandbuch deutsch und englisch
Installation Qualification-Dokument
AZURA
Netzkabel
Benutzerhandbuch deutsch und englisch
Installation Qualification-Dokument
BeipackDAD 2.1L
AZURA
MWD 2.1LDetektor
Netzkabel
Benutzerhandbuch deutsch und englisch
Installation Qualification-Dokument
BeipackMWD 2.1L
AZURA
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
12Sicherheit für Anwender
Sicherheit für Anwender
BerufsgruppeDas Benutzerhandbuch richtet sich an Personen, die mindestens
eine Ausbildung zum Chemielaboranten oder einen vergleichbaren Ausbildungsweg abgeschlossen haben.
Folgende Kenntnisse werden vorausgesetzt:
Grundlagenkenntnisse der Flüssigchromatografie
Kenntnisse über Substanzen, die nur bedingt in der Flüs-
sigchromatografie eingesetzt werden dürfen
Kenntnisse über die gesundheitlichen Risiken beim Umgang
mit Chemikalien
Teilnahme an der Installation eines Geräts oder einer Schu-
lung durch die Firma KNAUER oder einer von KNAUER autorisierten Firma
Gehören Sie nicht zu dieser oder einer vergleichbaren Berufsgruppe, dürfen Sie die in diesem Benutzerhandbuch beschriebenen Arbeiten auf keinen Fall ausführen. Informieren Sie in
diesem Fall Ihre Vorgesetzte oder Ihren Vorgesetzten.
SchutzausrüstungBei allen Arbeiten an dem Gerät sind die im Labor notwendigen
Schutzmaßnahmen zu beachten und folgende Schutzkleidung
zu tragen:
Schutzbrille mit zusätzlichem Seitenschutz
Schutzhandschuhe
Laborkittel
Was ist zu beachten? Alle Sicherheitshinweise im Benutzerhandbuch
Die Umgebungs-, Aufstell- und Anschlussbestimmungen im
Benutzerhandbuch
Bei der Arbeit mit Lösungsmitteln den Raum immer gut lüf-
ten
Nationale und internationale Vorschriften für das Arbeiten im
Labor
Vom Hersteller empfohlene oder vorgeschriebene Originaler-
satzteile, Werkzeuge und Eluenten
Good Laboratory Practice (GLP)
Unfallverhütungsvorschriften der Unfallkrankenkassen für
Labortätigkeiten
Aufreinigung der zu analysierenden Substanzen
Einsatz von Inline-Filtern
Keine gebrauchten Kapillaren an anderer Stelle im HPLC-Sys-
tem einsetzen
PEEK-Verschraubungen nur für ein- und denselben Port ver-
wenden oder grundsätzlich neue PEEK-Verschraubungen einsetzen
Hinweise von KNAUER oder anderer Hersteller zur Säulen-
pflege beachten
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
13Sicherheit für Anwender
Weitere für Ihre Sicherheit wichtige Themen sind in der folgenden Tabelle alphabetisch sortiert:
ThemaErläuterungen
EntflammbarkeitOrganische Eluenten sind leicht ent-
flammbar. Keine offenen Flammen in der
Nähe des Geräts betreiben, da Kapillaren
sich aus der Verschraubung lösen können, und dann eventuell leicht entflammbarer Eluent austritt.
FlaschenwanneEs besteht die Gefahr eines Stromschlags,
falls Eluenten oder andere Flüssigkeiten in
das Innere des Geräts gelangen. Deshalb
immer eine Flaschenwanne verwenden.
Flüssigkeits-
leitungen
Kapillare und Schläuche so verlegen, dass
beim Auftreten von Lecks austretende
Flüssigkeiten nicht in darunter angeordnete Geräte eindringen können.
LecksRegelmäßige Sichtkontrolle des Anwen-
ders auf Undichtigkeit im System wird
empfohlen.
NetzkabelBeschädigte Netzkabel dürfen nicht für
den Anschluss der Geräte an das Stromnetz benutzt werden.
Selbstent-
zündungs-
temperatur
Ausschließlich Eluenten verwenden, die
unter normalen Raumbedingungen eine
Selbstentzündungstemperatur höher als
150 °C haben.
SteckdosenleisteBeim Anschluss von mehreren Geräten an
eine einzige Steckdosenleiste immer die
maximal zulässige Stromaufnahme der
Geräte beachten.
StromversorgungGeräte dürfen nur an zugelassene Span-
nungsquellen angeschlossen werden,
deren Spannung mit der zulässigen Spannung des Geräts übereinstimmt.
ToxizitätOrganische Eluenten sind ab einer
bestimmten Konzentration toxisch.
Arbeitsraum immer gut belüften! Beim
Arbeiten am Gerät Schutzhandschuhe
und Schutzbrille tragen!
Wo darf das Gerät nicht
eingesetzt werden?
Das Gerät darf ohne besonderen und zusätzlichen Explosionsschutz nicht in explosionsgefährdeten Bereichen betrieben werden. Weitere Informationen erhalten Sie von der technischen
Kundenbetreuung von KNAUER.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
14Sicherheit für Anwender
Gerät sicher außer
Betrieb nehmen
Gerät öffnenGerät ausschließlich von der technischen Kundenbetreuung von
Das Gerät lässt sich jederzeit durch Ausschalten am Netzschalter
oder durch Lösen des Netzanschlusses vollständig außer Betrieb
nehmen.
KNAUER oder einer von KNAUER autorisierten Firma öffnen lassen.
Definition möglicher Personen- oder
Sachschäden
Möglichen Gefahren, die von einem Gerät ausgehen können,
werden in dem vorliegenden Benutzerhandbuch in Personenoder Sachschäden unterschieden.
KategorieErläuterungen
GEFAHR!Lebensgefahr oder sehr schwere Verletzun-
gen sind möglich.
WARNUNG!Schwere bis mittlere Verletzungen sind
möglich.
VORSICHT!Leichte bis sehr leichte Verletzungen sind
möglich. Ein Defekt des Geräts ist möglich.
Dekontamination
Die Kontamination von Geräten mit toxischen, infektiösen oder
radioaktiven Substanzen sind sowohl in Betrieb, bei der Reparatur, beim Verkauf als auch bei der Entsorgung eines Gerätes eine
Gefahr für alle Personen.
GEFAHR!
Gefahr durch den Kontakt mit toxischen, infektiösen oder radioaktiven Substanzen.
Bevor Geräte entsorgt oder zur Reparatur verschickt werden, müssen sie fachgerecht dekontaminiert werden.
Alle kontaminierten Geräte müssen von einer Fachfirma oder
selbständig fachgerecht dekontaminiert werden, bevor diese
wieder in Betrieb genommen, zur Reparatur, zum Verkauf oder
in die Entsorgung gegeben werden.
Alle zur Dekontamination verwendeten Materialien oder Flüssigkeiten müssen getrennt gesammelt und fachgerecht entsorgt
werden.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
15Symbole und Kennzeichen
Symbole und Kennzeichen
Die folgenden Symbole und Kennzeichen befinden sich am
Gerät, in der Chromatografie-Software oder im Benutzerhandbuch.
SymbolBedeutung
WarnzeichenGefährdung durch Hochspannung.
Gefährdung durch Stromschlag.
Gefährdung durch potentiell toxische
Substanzen.
Mögliche Gefährdung der Augen durch
UV-Licht.
Mögliche Verbrennungen beim Berühren
von heißen Bauteilen.
Hinweis auf eine Situation, die, wenn sie
nicht vermieden wird, zu leichten Verletzungen führen kann.
Hinweis auf eine Situation, die, wenn sie
nicht vermieden wird, zu Sachschäden
führen kann.
Möglicher Sachschaden durch elektrostatische Entladung am System, am Gerät
oder an bestimmten Bauteilen.
Maximale Gewichtsbelastung der Leckwanne während des Transports, der
Installation und im Betrieb beachten.
GebotszeichenSchutzhandschuhe schützen vor Haut-
schäden.
KonformitätszeichenDas mit dem CE-Zeichen gekennzeich-
nete Gerät oder System erfüllt die produktspezifisch geltenden europäischen
Richtlinien. Dies wird in der Konformitätserklärung bestätigt.
Das Prüfsiegel einer in Kanada und den
USA national anerkannten Prüfstelle
(NRTL). Das zertifizierte Gerät oder System hat die Prüfungen auf Qualität und
Sicherheit erfolgreich bestanden.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
Installation
16Installation
Das Kapitel Installation beschreibt diejenigen Handlungen, die
Sie vor der Inbetriebnahme beachten sollen. Wenn bei Ihnen
während der Installation Schwierigkeiten auftreten, setzen Sie
sich mit der Technischen Kundenbetreuung in Verbindung
Kontakt
Technischen Kunden-
betreuung
GerätedatenBerücksichtigen Sie für einen sicheren Transport das Gewicht
HebenDas Gerät seitlich am Gehäuse umfassen und aus der Verpa-
Telefon+49 30 809727-111
Fax+49 30 8015010
E-Mailsupport@knauer.net
Transport
Bereiten Sie das Gerät sorgfältig auf den Transport oder die
Lagerung vor. Wenn Sie das Gerät zur Reparatur an KNAUER
verschicken wollen, legen Sie den Servicebegleitschein bei, der
zum Download auf der Website bereitsteht.
und die Maße des Geräts (siehe Technische Daten).
VORSICHT!
Schäden am Gerät durch Heben an hervorstehenden Geräteteilen möglich!
Gerät ausschließlich seitlich am Gehäuse hochheben.
ckung heben.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
17Installation
1
Betriebsumgebung
Der bestimmungsgemäße Betrieb ist nur gewährleistet, wenn
Sie sich an die Vorgaben für die Umgebungsbedingungen des
Einsatzorts halten. Die Umgebungsbedingungen finden Sie im
Kapitel Technische Daten.
VORSICHT!
Gerätedefekt durch Überhitzung möglich!
Gerät vor Sonneneinstrahlung schützen.
Auf der Rückseite des Geräts mindestens 15 cm
und auf der Seite der Lüftungsschlitze mindestens 5–10 cm Platz für die Luftzirkulation lassen.
Aufstellort und Platzbedarf
Das Gerät auf eine ebene und gerade Fläche stellen.
Sonneneinstrahlung: Das Gerät vor direkter Sonneneinstrah-
lung schützen.
Klimaanlage: Das Gerät frei von Zugluft aufstellen.
Vibration: Das Gerät nicht neben Maschinen platzieren, die
Bodenvibrationen verursachen.
Seitlicher Abstand zu weiteren Geräten:
Mindestens 5 cm, wenn auf einer Seite ein weiteres Gerät
aufgestellt wird
Mindestens 10 cm, wenn auf beiden Seiten ein weiteres
Gerät aufgestellt wird
Netzstecker auf der Geräte-Rückseite frei zugänglich hal-
ten, damit das Gerät vom Stromnetz getrennt werden
kann.
Gerät von Hochfrequenzquellen fern halten. Hochfrequenz-
quellen können die Messwerte beeinflussen.
ErdbebengebietIn Erdbebengebieten das Gerät an den Fixierungspunkten
befestigt. Die Fixierungspunkte befinden sich rechts oder links
am Gerät.
Abb. 5Fixierungspunkte am Gerät
1
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
18Installation
Platzbedarf
Seitlicher Abstand zu weiteren Geräten:
mindestens 5 cm, wenn auf einer Seite ein weiteres Gerät
aufgestellt ist.
mindestens 10 cm, wenn auf beiden Seiten ein weiteres
Gerät aufgestellt ist.
Mindestabstand 15 cm zum Lüfter auf der Geräterückseite.
Aufbau
Bevor Sie das Gerät aufbauen, sollen Sie einen geeigneten Einsatzort gemäß der Vorgaben auswählen und die Verpackung
entfernen. Die Vorgaben und eine Handlungsbeschreibung finden Sie im nachfolgenden Abschnitt.
Auspacken
VoraussetzungDer Karton wurde auf Transportschäden geprüft.
WerkzeugCuttermesser
Ablauf
1. Die Verpackung so aufstellen, dass die Schrift am Etikett
richtig herum steht. Das Klebeband mit einem Cuttermesser durchtrennen und die Verpackung öffnen.
2. Die Schaumstoffauflage abheben. Die Zubehörteile und
das Benutzerhandbuch herausnehmen.
3. Die Zubehörteile aus der Tüte nehmen und den Lieferumfang prüfen. Im Fall einer unvollständigen Lieferung die
Technische Kundenbetreuung kontaktieren.
4. Das Gerät von unten umfassen, aus der Verpackung heben
und auf den Füßen abstellen. Dabei nicht an der vorderen
Abdeckung festhalten.
5. Das Gerät auf Transportschäden prüfen. Im Fall einer
Beschädigung die Technische Kundenbetreuung kontaktieren.
6. Das Gerät am Einsatzort platzieren und die Schutzfolien
abziehen.
VORSICHT!
Schäden am Gerät durch Heben an hervorstehenden Geräteteilen möglich!
Gerät ausschließlich seitlich am Gehäuse hochheben.
Nächste SchritteLagern Sie Karton und Verpackung und bewahren Sie die beilie-
gende Packliste für spätere Nachbestellungen auf.
Hinweis Die Testzelle wird für Funktionstests benötigt und muss sorgfäl-
tig aufbewahrt werden.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
19Installation
Durchflusszelle einsetzen
Voraussetzung Es ist keine Durchflusszelle oder eine Testzelle eingesetzt.
Das Gerät ist ausgeschaltet.
Vorgehensweise Die Durchflusszelle einsetzen
Hinweis Achten Sie darauf, dass kompatible Durchflusszellen eingesetzt
werden.
WARNUNG!
Reizung der menschlichen Netzhaut durch UVLicht. Gebündeltes UV-Licht kann an der Durchflusszelle oder am Lichtwellenleiter austreten.
Das Gerät oder die Lampen ausschalten!
VorgehensweiseAblaufAbbildung
1. Die Durchflusszelle in
die Öffnung setzen und
nach hinten schieben
bis sie einrastet.
Nächste SchritteSchließen Sie die Kapillaren an.
Durchflusszelle mit Lichtwellenleiter einbauen
Sie können die Durchflusszelle auch mit Lichtwellenleitern verbinden. Die Lichtwellenleiter mit der Durchflusszelle werden an
einen Adapter angeschlossen.
UV-Licht lässt Lichtwellenleiter im Laufe der Zeit solarisieren,
sodass diese für den Einsatz nicht mehr geeignet sind.
Beim Benutzen von Lichtwellenleitern folgende Punkte beachten:
Die Enden des Lichtwellenleiters nicht mit den Fingern berüh-
ren, da dies zu fehlerhaften Messungen führen könnte.
Stöße und hartes Aufschlagen des Lichtwellenleiters vermei-
den.
Lichtwellenleiter vorsichtig ohne Druck und ohne Verbiegen
bewegen.
Abb. 6Durchflusszelle einset-
zen
VoraussetzungenEs ist keine Durchflusszelle oder Testzelle eingesetzt.
HilfsmittelBlindverschraubungen für die Lichtwellenleiter.
WARNUNG!
Reizung der menschlichen Netzhaut durch UVLicht. Gebündeltes UV-Licht kann an der Durchflusszelle oder am Lichtwellenleiter austreten.
Das Gerät oder die Lampen ausschalten!
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
20Installation
1
1
1
3
Vorgehensweise
AblaufAbbildung
1. Die Endstücke
LWL-Adapter abziehen.
2. Den LWL-Adapter in die
Öffnung setzen und
nach hinten schieben
bis er einrastet.
3. Die Blindverschraubungen
1 von den Enden
des Lichtwellenleiter
abziehen.
4. Die Verschraubungen
der Lichtwellenleiter
von Hand am LWL-
Adapter
ben.
5. Die Endstücke der
Durchflusszelle abziehen.
6. Die Verschraubungen
1 am
Abb. 7LWL-Adapter
Abb. 8Blindverschraubung der
Lichtwellenleiter
2 anschrau-
3 der Lichtwellenleiter
an der Durchflusszelle
von Hand anschrauben.
Abb. 9Verschraubungen der
Lichtwellenleiter an der
Durchflusszelle
Nächste SchritteSchließen Sie die Kapillaren an.
Kapillare anbringen
Die Kapillaren verbinden den Detektor mit der Säule und leiten
die Flüssigkeiten. Für die Anschlüsse der Durchflusszelle empfehlen wir PEEK-Kapillare und PEEK-Verschraubungen.
VORSICHT!
Schäden an den PEEK-Gewinden der Durchflusszelle durch Metall-Verschraubungen möglich.
Kapillaren ausschließlich mit PEEK-Verschraubungen anbringen.
Drehmoment von maximal 0,5 Nm beachten.
Voraussetzung Die Durchflusszelle ist eingesetzt.
Blindverschraubungen und Endstücke sind entfernt.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
21Installation
132
4
13
2
4
4
4
VorgehensweiseDie Kapillare mit Verschraubung anbringen.
Vorgehensweise
AblaufAbbildung
1. Kapillare
Verschraubung
schieben.
2. Den Klemmring
den Schlauch schie-
ben. Bei der Ausrichtung des Klemmrings
darauf achten, dass die
angeschrägte Seite in
Richtung Dichtring
zeigt.
3. Den Dichtring
cken.
4. Kapillare an der Durchflusszelle
verschrauben.
1 durch die
2
3 über
4
4aufste-
4 handfest
Abb. 10 Kapillarverschraubun-
gen
Abb. 11 Durchflusszelle mit
Nächste SchritteNehmen Sie das Gerät in Betrieb.
Einsatz von PEEK-Verschraubungen
PEEK-Verschraubungen können bis zu einem Druck von 400 bar
verwendet werden. Beachten Sie den Drehmoment von
0,5 Nm.
Leckmanagement anschließen
Das Leckmanagement setzt sich aus einem Lecksensor und
einem Drainagesystem zusammen. Das Drainagesystem sorgt
dafür, dass ausgetretene Flüssigkeiten automatisch in einen
Abfallbehälter fließen. Wenn sich zu viel Flüssigkeit ansammelt,
blinkt die rote LED am Gerät. Das Gerät und die Datenaufnahme
über die Chromatograpfiesoftware werden gestoppt.
Kapillare
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
22Installation
12
3
4
5
6
Voraussetzung Die Frontabdeckung wurde abgenommen.
Vorgehensweise
AblaufAbbildung
1. Den Trichter
mittlere Öffnung der
Kapillarführung
cken.
2. Die Schlauchstutzen 4
an der langen Seite in
den Drainageschlauch
1 in die
2 ste-
Abb. 12 Trichter mit Kapillarfüh-
rung
3 stecken.
Abb. 13 Drainageschlauch mit
Schlauchstutzen
3. Die Schlauchstutzen an
den Trichter stecken.
4. Den Drainageschlauch
mit dem Schlauchstut-
zen
5 an der Leck-
wanne verbinden.
Abb. 14 Drainageschlauch am
Gerät befestigen
5. Am untersten Gerät
den Abfallstutzen
befestigen.
6. Den Abfallschlauch am
Abfallstutzen befestigen
und mit dem Abfallbehälter verbinden.
7. Den Abfallbehälter
unterhalb der Geräte
aufstellen.
Nächste SchritteBefestigen Sie die Frontabdeckung.
6
Abb. 15 Leckwanne mit Abfall-
stutzen
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
Legende
1423
5
1 Integratorausgänge
2 Anschluss LAN
3 Schnittstelle für den
Service
23Installation
Steuerung
Der Detektors wird auf 2 Arten extern gesteuert:
Über die Stiftleiste
Innerhalb eines lokalen Netzwerks über den LAN-Anschluss
an den Router.
Sämtliche Anschlüsse für die externe Steuerung befinden sich
auf der Rückseite des Detektors
Eine lokale Ansteuerung des DAD 6.1L über die Control Unit ist
ebenfalls möglich. Der Anschluss für die Control Unit befindet
sich an der Seite des Geräts.
.
4 Stiftleiste
DAD 6.1L
1 USB-Anschluss für
die Control Unit
Abb. 16 Anschlüsse Rückseite
Abb. 17 Anschluss Control Unit am DAD 6.1L
Gerät an den Computer anschließen
Dieses Kapitel beschreibt, wie ein HPLC-System zu einem lokalen
Netzwerk (LAN) aufgebaut wird und wie das LAN durch einen
Netzwerkadministrator zum Datenaustausch an ein Firmennetzwerk angeschlossen werden kann. Die Beschreibung gilt für das
Betriebssystem Windows® und alle gängigen Router.
Hinweis Um ein LAN aufzubauen, wird die Verwendung eines Routers
empfohlen. Das heißt, dass folgende Schritte erforderlich sind:
Ablauf1. Am Computer in der Systemsteuerung die LAN-Eigenschaften
prüfen.
2. Den Router mit den Geräten und dem PC verkabeln.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
3. Am Computer den Router für das Netzwerk einrichten.
24Installation
4. Die Chromatografiesoftware vom entsprechenden Datenträger installieren.
5. Die Geräte einschalten und Chromatografiesoftware starten.
LAN-Eigenschaften einstellen
Im LAN wird ausschließlich ein Server (im Regelfall der Router)
verwendet, von dem die Geräte automatisch ihre IP-Adresse im
Netzwerk beziehen.
Voraussetzung In Windows
Standby-Funktion und Bildschirmschoner ausgeschaltet.
Wenn eine “USB to COM”-Box verwendet wird, muss im
Gerätemanager die Einstellung “Computer kann das Gerät
ausschalten, um Energie zu sparen” für alle USB-Hosts deaktiviert werden.
Nur Windows 7: Für den Netzwerkadapter im Gerätemana-
ger die Einstellung “Computer kann das Gerät ausschalten,
um Energie zu sparen” deaktivieren.
®
sind Energiesparfunktionen, Ruhezustand,
Vorgehensweise1. In Windows 7 Start
gabecenter auswählen.
2. Auf LAN-Verbindung doppelklicken.
3. Die Schaltfläche Eigenschaften anklicken.
4. Internetprotokoll Version 4 (TCP/IPv4) auswählen.
5. Die Schaltfläche Eigenschaften anklicken.
6. In der Registerkarte Allgemein die Einstellungen prüfen. Die
korrekten Einstellungen des DHCP-Clients sind:
a) IP-Adresse automatisch beziehen
b) DNS-Serveradresse automatisch beziehen
7. Die Schaltfläche OK anklicken.
Geräte zum LAN verkabeln
Der Router hat mehrere LAN-Anschlüsse und einen WAN-/Internetanschluss, über den der Router an ein Wide Area Network
(WAN) angeschlossen werden kann, wie z. B. ein Firmennetzwerk oder das Internet. Die LAN-Anschlüsse dagegen dienen
zum Aufbau eines Netzwerks aus Geräten und Computer. Um
Störungen zu vermeiden wird empfohlen, das HPLC-System
außerhalb des Firmennetzwerks zu betreiben.
Für jedes Gerät und für den Router wird ein Patch-Kabel mitgeliefert. Um den Router an das Netzwerk anzuschließen, wird ein
Systemsteuerung Netzwerk- und Frei-
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
25Installation
12
3
4
5
zusätzliches Patch-Kabel benötigt, das nicht im Lieferumfang
enthalten ist.
Legende
1 Geräte
2 Router
4 WAN-/Internetanschluss
5 Workstation
3 LAN-Anschlüsse
Abb. 18 Kabelplan LAN
Voraussetzung Der Computer wurde ausgeschaltet.
Für die Geräte und den Computer ist je ein Patch-Kabel vor-
handen.
Vorgehensweise1. Mit dem Patch-Kabel den Router und den Computer verbin-
den. Diesen Schritt wiederholen, um die Geräte anzuschließen.
2. Mit dem Netzteil den Router an das Stromnetz anschließen.
Vorgehensweise1. Um die Routerkonfiguration zu öffnen, im Browser die IP-
Router einstellen
Der Router wird mit werkseitigen Voreinstellungen ausgeliefert.
Auf der Unterseite des Routers ist ein Aufkleber angebracht, auf
dem die IP-Adresse, Benutzername und Passwort zu finden sind,
mit denen man die Routerkonfiguration öffnen kann.
Adresse des Routers eingeben (gilt nicht für alle Router).
2. Den Benutzernamen und das Passwort eingeben.
3. Den Router als DHCP-Server einstellen.
4. In der Routerkonfiguration den IP-Adressbereich prüfen und
ggf. ändern.
ErgebnisSobald der Router allen Geräten eine IP-Adresse zugewiesen hat,
übernimmt die Chromatografiesoftware die Steuerung des
HPLC-Systems.
LAN in das Firmennetzwerk integrieren
Der Router kann durch den Netzwerkadministrator an das Firmennetzwerk angeschlossen werden. Dazu wird der WAN-/
Internetanschluss des Routers verwendet.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
26Installation
VoraussetzungDas Patch-Kabel ist vorhanden.
Vorgehensweise1. Prüfen, dass es keine Überschneidung zwischen den IP-Adres-
sen des Routers und des Firmennetzwerks gibt.
2. Im Fall einer Überschneidung in der Routerkonfiguration den
IP-Adressbereich ändern.
3. Mit dem Patch-Kabel den WAN-/Internetanschluss des Routers mit dem Firmennetzwerk verbinden.
4. Alle Geräte einschließlich des Computers neu starten.
Mehrere Systeme in einem LAN separat steuern
Die Kommunikation in LANs läuft über sogenannte Ports, die
Teil der Netzwerkadresse sind. Wenn in einem LAN mehrere
HPLC-Systeme vernetzt sind, die separat gesteuert werden sollen, können dafür unterschiedliche Ports verwendet werden, um
Störungen zu vermeiden. Dafür muss die Portnummer an jedem
Gerät geändert und die gleiche Portnummer in der Gerätekonfiguration der Chromatografiesoftware eingegeben werden. Es
empfiehlt sich, für alle Geräte eines Systems dieselbe Portnummer zu verwenden.
Hinweis Der Port ist bei allen Geräten werkseitig auf 10001 eingestellt.
Die Portnummern in der Konfiguration der Geräte in der Chromatografiesoftware und am Gerät müssen identisch sein,
ansonsten kann keine Verbindung hergestellt werden.
Vorgehensweise1. Die Portnummer bestimmen und am Gerät ändern.
2. Die Portnummer in der Chromatografiesoftware eingeben.
ErgebnisDie Verbindung wird hergestellt.
Anschlüsse der Stiftleiste
Bei bestimmten Anlässen oder zu Testzwecken kann die manuelle Eingabe von Signalen zweckmäßig sein:
Senden von Steuersignalen (Events) an externe Geräte
Öffnen und Schließen von Kontakten
Aktivierung von 500 ms-Impulsen
Folgende Remote Signale können empfangen und gesendet
werden:
Empfang von Start- und Error-Signalen externer Geräte
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
27Installation
Senden von Steuer- und Error-Signalen an externe Geräte
Abb. 19 Beziehung zwischen Anzeige und Anschlussleiste
Erläuterungen zur Stiftleiste
AnschlussFunktion
Relaiskontakt
EV 1
(Event 1)
Der Kontakt ist potentialfrei. Die Einstellung ist abhängig von den Einstelungen in der Control Unit, der Mobile
Control oder der Software.
Dauersignal:
passiv = Relaiskontakt geöffnet
aktiv = Relaiskontakt geschlossen
Impuls:
Relaiskontakt geschlossen für
min. 1000 ms
Zulässige Belastung des Relaiskontakts:
1 A/ 24 V DC
EV 2 (Event 2)TTL-Ausgang
Pegel:
passiv 5 V
aktiv 0 V
Impuls:
0 V für min. 1000 ms
Error OUTTTL-Ausgang
Pegel:
passiv 5 V
aktiv 0 V
Der Ausgang ist aktiv, bis die ErrorBedingung entfällt.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
28Installation
AnschlussFunktion
Error INTTL-Eingang
Start INTTL-Eingang
Low-aktiv
Sichere Schaltschwelle min. 10 mA
Bei einem Signal (Kurzschluss nach
GND) von einem externen Gerät,
erscheint eine Fehlermeldung.Das
Gerät stoppt.
Low-aktiv
Sichere Schaltschwelle min. 10 mA
Bei einem Signal (Kurzschluss nach
GND) von einem externen Gerät startet das Gerät. Bei Softwarekontrolle
wird ein elektronischer Trigger per
LAN verschickt.
AutozeroTTL-Eingang
Low-aktiv
Sichere Schaltschwelle min. 10 mA
Ein Signal (Kurzschluss nach GND)
stellt das aktuelle Messsignal auf Null.
+5 VStellt eine Spannung von 5 V gegen
GND zur Verfügung. Damit kann ein
mit Event geschalteter Verbraucher
versorgt werden.
Absicherung: 5 V–50 mA
GNDBezugspunkt der Spannung an den
Signaleingängen.
+24V ValveEvent-gesteuerte Spannung von 24 V
gegen GND.
Absicherung: 24 V–200 mA
Valve OUTAusgang ist aktiv, bis die Valve-Bedin-
gung entfällt.
GNDBezugspunkt der Spannung an den
Signaleingängen.
Stiftleiste verkabeln
Um ein Gerät durch ein anderes Gerät anzusteuern, wird ein
Industriesteckverbinder verwendet. Über die einzelnen
Anschlüsse werden Steuersignale übertragen.
Um Geräte fernzusteuern, müssen Sie die Kabel an die Stiftleiste
anschließen. Die Stifte der Stiftleiste stecken Sie abschließend
auf die Federkontakte der Federleiste, über die eine Verbindung
zur Platine hergestellt wird.
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
29Installation
1
2
3
Voraussetzung Das Gerät wurde ausgeschaltet.
Der Netzstecker wurde gezogen.
WerkzeugHebeldrücker
VORSICHT!
Beschädigung des Geräts durch Kurzschluss möglich!
Gerät vor dem Anschließen an die Stiftleiste ausschalten. Netzstecker ziehen.
VORSICHT!
Zerstörung der Elektronik durch elektrostatische
Entladung!
VorgehensweiseAblaufAbbildung
Geerdetes Armband tragen.
1. Die Stiftleiste
Unterlage legen.
2. Den Hebeldrücker
die Öffnung auf der Oberseite stecken und nach
unten drücken.
3. Den Hebeldrücker
gedrückt halten und die
Kabel
2 in die Vorder-
seite einführen. Danach
den Hebeldrücker herausziehen.
4. Prüfen, ob die Kabel fest
verbunden sind.
5. Die Stiftleiste auf die
Federleiste drücken.
Nächste SchritteBeenden Sie die Installation. Nehmen Sie danach das Gerät in
Betrieb.
3 auf eine
1 in
Abb. 20 Stiftleiste
Integrator-Anschluss
Der Integrator-Anschluss gibt Messsignale des Detektors aus.
nicht bipolar
4 Kanal
0 bis 5 V
DAC 16 bit
skalierbar
Offset einstellbar
KNAUERDAD 6.1L, DAD 2.1L, MWD 2.1L Benutzerhandbuch V6700, Version 1.0
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